Infectious Pancreatic Necrosis Virus (IPNV) - Fróðskaparsetur Føroya

Content
1 Introduction .................................................................................................................................. 4
1.1 Background .............................................................................................................................................. 4
1.2 Problem Statement .................................................................................................................................. 5
1.3 Experimental Design ............................................................................................................................... 5
1.3.1 Description of the Processing Plant........................................................................................................ 6
1.3.2 Sampling Coverage ................................................................................................................................ 6
2 Theories ......................................................................................................................................... 8
2.1 Listeria monocytogenes and Listeriosis .................................................................................................. 8
2.1.1 Listeria monocytogenes .......................................................................................................................... 8
2.1.2 Listeriosis ............................................................................................................................................... 9
2.1.3 Listeriosis Caused by Food .................................................................................................................. 10
2.1.4 Trade Problems .................................................................................................................................... 11
2.2 Appearance of Listeria monocytogenes in Food, Especially Fish ...................................................... 12
2.3 Occurrence of Listeria monocytogenes in Seawater ............................................................................ 14
2.4 Occurrence and Tracking of Listeria monocytogenes in Production ................................................ 16
2.5 Persistency and Control of Listeria monocytogenes ............................................................................ 16
3 Methods ....................................................................................................................................... 18
3.1 Samples................................................................................................................................................... 18
3.1.1 Materials ............................................................................................................................................... 18
3.1.2 Samples: Production Equipment, Raw Material, Manufactured Goods and Seawater Samples .......... 18
3.2 Listeria monocytogenes Identification .................................................................................................. 19
3.2.1 Listeria monocytogenes Enrichment .................................................................................................... 19
3.2.2 Listeria monocytogenes Isolation ......................................................................................................... 19
3.2.3 Listeria monocytogenes Identification ................................................................................................. 20
3.3 Multilocus Sequence Typing (MLST) of Listeria monocytogenes ..................................................... 20
3.3.1 Phylogenetic Analysis using ClustalX and TreeView.......................................................................... 21
4 Results .......................................................................................................................................... 22
5 Discussion .................................................................................................................................... 26
6 Conclusion ................................................................................................................................... 31
7 Perspective ................................................................................................................................... 32
8 References.................................................................................................................................... 33
9 Appendix ..................................................................................................................................... 38
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