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¥Agronomia vol. 6 n. 2 July

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122 Baldini et al.<br />

Analysis of variance was done on the data, and<br />

when the F test proved significant, Duncan’s<br />

test at P ≤ 0.05 was used to separate the mean<br />

values of the treatments. For the data from the<br />

samples taken during the crop cycle the sources<br />

of variation were water regime and sampling<br />

date in the first year, and water regime, hybrid<br />

and sampling date in the second.<br />

RESULTS AND DISCUSSION<br />

Figure 1 shows achene dry matter accumulation<br />

during maturation. As the statistical analysis<br />

demonstrated that the hybrid effect was not significant,<br />

the values reported are the means of<br />

the two hybrids. It should be pointed out that<br />

at the first sampling (two days after the end of<br />

flowering), the embryo begins to form and<br />

therefore all the values relating to that date refer<br />

principally to the tissues of the seed coat,<br />

which forms and develops independently of fertilisation.<br />

The most significant increase in dry<br />

weight took place between 8 and 14 days after<br />

flowering, while between 20 and 28 days dry<br />

matter accumulation in the achene is more or<br />

less completed. Between treatments, water<br />

stress determined the formation of lighter achenes<br />

at maturity compared to the other treatments,<br />

with a higher growth rate in the earliest<br />

stages (8 days after fertilisation), thus confirming<br />

the findings of Hall et al. (1985). Figure 2<br />

shows a strong increase in oil accumulation in<br />

the achene (the values are the mean of the two<br />

hybrids), between the 8th and 14th days after fertilisation,<br />

in correspondence to the significant<br />

Figure 1. 1998. Time course of dry matter accumulation in<br />

achenes of plants submitted to water table (), ET () and<br />

stress () water regimes. The values reported refer to the<br />

mean of the two cv. The vertical bars represent the standard<br />

error of the mean.<br />

Figure 2. 1998. Time course of oil accumulation in achenes<br />

of plants submitted to water table (), ET () and stress<br />

() water regimes. The values reported refer to the mean<br />

of the two cv. The vertical bars represent the standard error<br />

of the mean.<br />

increase in dry matter in the achene (Figure 1).<br />

Among water regimes, the supply from the water<br />

table determined, at maturity, significantly<br />

higher percentages of oil in the achene than in<br />

the other irrigation regimes. However, all treatments<br />

reached the maximum oil content on the<br />

20 th day after the end of flowering, confirming<br />

what Champolivier and Merrien (1996) found<br />

in a trial conducted in a phytotron at the highest<br />

temperatures (27 °C day and 22 °C night),<br />

which are roughly equivalent to those at the trial<br />

site during post-flowering (Table 1).<br />

Figures 3 and 4 give the accumulation of the<br />

major fatty acids in the oil of the high oleic and<br />

standard hybrids for both years.<br />

In the first year of the trial the saturated fatty<br />

acids content (palmitic and stearic) in the high<br />

oleic hybrid diminished rapidly in the days following<br />

the end of flowering, to then stabilise between<br />

the 17 th and 23 rd day at approx. 2.5 and<br />

4%, respectively (Figure 3). The oleic acid content<br />

at the first sampling (74%) was significantly<br />

lower than that found at full-ripening (85%),<br />

while linoleic acid, on the contrary, had a value<br />

of 10% at the first sampling against 5% at maturity.<br />

Both these fatty acids stabilised between<br />

the 17 th and 23 rd day after the end of flowering<br />

(Figure 3).<br />

In the second year the palmitic acid trend<br />

seemed analogous in the two hybrids, with a<br />

slightly higher final amount in the standard hybrid<br />

(5.5%) than in the high oleic one (3%).<br />

This fatty acid reduced, compared to the initial

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