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<strong>EMBO</strong> Plant DNA Repair and Recombination Workshop, Presqu'île de Giens, France 2007 Page 28 sur 56
<strong>EMBO</strong> Plant DNA Repair and Recombination Workshop, Presqu'île de Giens, France 2007 Abstracts of Posters ---------------------------------------------------------------------------------------------------------------------------------- P - 1. Analysis of Meiotic Recombination in Rad51 Paralog Mutants in Arabidopsis. Kiyomi Abe*, Jean-Yves Bleuyard, Charles White.. CNRS UMR6547, Université Blaise Pascal, 63177 Aubière, France. *Plant Genetic Engineering Research Unit, National Institute of Agrobiological Sciences, 305-8602 Tsukuba, Japan Recent studies indicate that two Rad51 paralogs (Xrcc3 and Rad51C) are involved in both DNA repair and meiotic recombination. To determine further details about the role of these proteins in meiotic recombination, we analyzed meiosis in atrad51C and xrcc3 mutants. Cytological observation showed apparently normal figures up to zygotene/pachytene and dramatic abnormalities afterwards through telophase I in both atrad51C and xrcc3 mutants, indicating that pairing does occur at zygotene but that full chromosomal synapsis and synaptonemal complex formation depends upon the presence of the Xrcc3 and Rad51C proteins. Using fluorescence in situ hybridization (FISH) analysis we observe pairing of centromeres but not of the tested euchromatic region at pachytene in both mutants. These results suggest that Xrcc3 and Rad51C proteins play important roles in homologous pairing of the euchromatic regions of chromosomes. ---------------------------------------------------------------------------------------------------------------------------------- P - 2. Genome-wide analysis of rye specific chromosome regions Olena Alkhimova, Marie Kubalakova, Olena Martynenko, Jaroslav Dolezel . National Academy of Sciences of Ukraine, Institute of Molecular Biology and Genetics, 03143 Kyiv, Ukraine Telomeres are specialized chromosome structure, which play important role in chromosome stability and behaviour. Together with subtelomeres, they form terminal regions of chromosomes. These are dynamic regions and might help organisms to adapt to new environmental conditions and promote genomic rearrangements. Large heterochromatic blocks, which are present near the telomeres of rye chromosomes, are notoriously difficult for physical mapping because of enrichment by different types of repetitive DNA sequences. We used wheat-rye addition lines and performed fluorescence in situ hybridization (FISH) on metaphase stretched flow-sorted chromosomes and extended DNA fibers for comparison of the terminal regions of the individual rye chromosomes. Each wheat-rye addition line produced a chromosome-specific large-scale organization of the tandem repeat arrays. The FISH signals on DNA fibers showed the multiple patterns of co-linear monomers arrangement of repetitive families as well as of authentic telomeric probe from Arabidopsis. The primary structure of some spacer sequences revealed the scrambled regions of similarity to various known repetitive elements. Using PCR analysis, we determined the DNA sequences adjacent to the tandem repeats array (spacers). They are enriched of short direct, complementary, inverted and symmetrical repeats which appear to be associated with recombination events. These spacers may be a powerful source of tandem array rearrangements. We mapped some of them on metaphase stretched chromosomes, and the distribution of the signals was in good agreement with the localization of the breakpoints for deletions and for translocations of 1R short arm with different wheat chromosomes. This work was supported by the INTAS grant (03-51-5908). ---------------------------------------------------------------------------------------------------------------------------------- Page 29 sur 56
Page 1: PLANT DNA Repair & Recombination In
Page 4 and 5: EMBO Plant DNA Repair and Recombina
Page 6 and 7: 1400-1700 SESSION V: Recombination
Page 20 and 21: eing propagated. EMBO Plant DNA Rep
Page 54 and 55: Cyril Charbonnel CNRS UMR6547 Unive
Page 56 and 57: Sean Mayes University of Nottingham
Page 58: Wanda Melody Waterworth University

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