PWD BK W7000-893 Proceedings.CDR - Mountain Lion Foundation
PWD BK W7000-893 Proceedings.CDR - Mountain Lion Foundation
PWD BK W7000-893 Proceedings.CDR - Mountain Lion Foundation
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sixth <strong>Mountain</strong> <strong>Lion</strong> w o r k s h o p 57<br />
A PHYLOGEOGRAPHIC STUDY OF PUMAS<br />
(PUMA CONCOLOR) USINGMITOCHONDRIAL DNA<br />
MARKERS AND MICROSATELLITES<br />
MELANIE CULVER*, Virginia Polytechnic Institute and State University, Department of Fisheries and<br />
Wildlife Sciences, Blacksburg, VA.<br />
WARREN JOHNSON, Genetics Section, Laboratory of Genomic Diversity, National Cancer Institute,<br />
Frederick Cancer Research and Development Center, Frederick, MD.<br />
JILL PECON-SLATTERY, Genetics Section, Laboratory of Genomic Diversity, National Cancer Institute,<br />
Frederick Cancer Research and Development Center, Frederick, MD.<br />
STEPHEN J. O'BRIEN, Genetics Section, Laboratory of Genomic Diversity, National Cancer Institute,<br />
Frederick Cancer Research and Development Center, Frederick, MD.<br />
Abstract: In this research project, several molecular<br />
markers were used to ascertain the level of<br />
genetic differentiation among natural puma<br />
(Puma concolor) populations, and also use this<br />
differentiation to understand genetic structure and<br />
infer natural history and evolution of the puma.<br />
Samples were obtained from throughout the<br />
geographical multi-habitat range of pumas and<br />
encompass all 32 described subspecies. Population<br />
level genetic differentiation in the puma was<br />
assessed using 2 independent molecular markers.<br />
These are several regions of mitochondrial DNA<br />
and 10 feline nuclear microsatellites. Results from<br />
both mirochondrial and nuclear markers indicate a<br />
low level of genetic variation in North American<br />
pumas relative to abundant variation observed in<br />
South American pumas. Regional differences are<br />
observed for Central and South American pumas<br />
with a total of 6 phylogeographic groups identified<br />
using both mitochondrial and nuclear markers.<br />
Furthermore, extant puma lineages appear to have<br />
originated in South America. The North American<br />
genetic lineage is younger than the South<br />
American lineages and younger than the North<br />
American fossil record. This indicates the potential<br />
occurrence of an historic extinction and recolonization<br />
event among North American pumas.<br />
TWO SIMPLE METHODS OF HANDLING RADIO-<br />
LOCATION ERROR FOR WIDE-RANGING ANIMALS IN<br />
LARGE COMPLEX STUDY AREAS<br />
BRETT G. DICKSON*, School of Forestry, Northern Arizona University, Flagstaff, AZ 86001.<br />
PAUL BEIER, School of Forestry, Northern Arizona University, Flagstaff, AZ 86001.<br />
Abstract: The study of second-order habitat<br />
selection (how an animal selects a home range<br />
within a larger area of potential habitat) analyzes<br />
the differences between habitats used by an<br />
animal versus habitats available on the study area.<br />
Most studies of cougar (Puma concolor) habitat<br />
use assigned each radio-location to a single<br />
vegetation polygon, ignoring the fact that radiolocations<br />
are imprecise estimates of the animal's<br />
true location (we refer to this as "naive estimator" of<br />
habitat use). Herein, we describe 2 simpler alterna-<br />
tive procedures to accommodate location error,<br />
readily implemented in a GIS. One alternative<br />
("circular error estimator") is to treat each point as<br />
the center of a circle with radius equal to the<br />
estimated average error, and assign each location<br />
to habitat types with probability proportional to<br />
area of that habitat type within the circle. Another<br />
alternative ("nonpoint estimator") is to use estimated<br />
locations solely to construct a home range<br />
contour and compare the habitat composition of<br />
the home range (rather than of individual points)