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PWD BK W7000-893 Proceedings.CDR - Mountain Lion Foundation

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sixth <strong>Mountain</strong> <strong>Lion</strong> w o r k s h o p 57<br />

A PHYLOGEOGRAPHIC STUDY OF PUMAS<br />

(PUMA CONCOLOR) USINGMITOCHONDRIAL DNA<br />

MARKERS AND MICROSATELLITES<br />

MELANIE CULVER*, Virginia Polytechnic Institute and State University, Department of Fisheries and<br />

Wildlife Sciences, Blacksburg, VA.<br />

WARREN JOHNSON, Genetics Section, Laboratory of Genomic Diversity, National Cancer Institute,<br />

Frederick Cancer Research and Development Center, Frederick, MD.<br />

JILL PECON-SLATTERY, Genetics Section, Laboratory of Genomic Diversity, National Cancer Institute,<br />

Frederick Cancer Research and Development Center, Frederick, MD.<br />

STEPHEN J. O'BRIEN, Genetics Section, Laboratory of Genomic Diversity, National Cancer Institute,<br />

Frederick Cancer Research and Development Center, Frederick, MD.<br />

Abstract: In this research project, several molecular<br />

markers were used to ascertain the level of<br />

genetic differentiation among natural puma<br />

(Puma concolor) populations, and also use this<br />

differentiation to understand genetic structure and<br />

infer natural history and evolution of the puma.<br />

Samples were obtained from throughout the<br />

geographical multi-habitat range of pumas and<br />

encompass all 32 described subspecies. Population<br />

level genetic differentiation in the puma was<br />

assessed using 2 independent molecular markers.<br />

These are several regions of mitochondrial DNA<br />

and 10 feline nuclear microsatellites. Results from<br />

both mirochondrial and nuclear markers indicate a<br />

low level of genetic variation in North American<br />

pumas relative to abundant variation observed in<br />

South American pumas. Regional differences are<br />

observed for Central and South American pumas<br />

with a total of 6 phylogeographic groups identified<br />

using both mitochondrial and nuclear markers.<br />

Furthermore, extant puma lineages appear to have<br />

originated in South America. The North American<br />

genetic lineage is younger than the South<br />

American lineages and younger than the North<br />

American fossil record. This indicates the potential<br />

occurrence of an historic extinction and recolonization<br />

event among North American pumas.<br />

TWO SIMPLE METHODS OF HANDLING RADIO-<br />

LOCATION ERROR FOR WIDE-RANGING ANIMALS IN<br />

LARGE COMPLEX STUDY AREAS<br />

BRETT G. DICKSON*, School of Forestry, Northern Arizona University, Flagstaff, AZ 86001.<br />

PAUL BEIER, School of Forestry, Northern Arizona University, Flagstaff, AZ 86001.<br />

Abstract: The study of second-order habitat<br />

selection (how an animal selects a home range<br />

within a larger area of potential habitat) analyzes<br />

the differences between habitats used by an<br />

animal versus habitats available on the study area.<br />

Most studies of cougar (Puma concolor) habitat<br />

use assigned each radio-location to a single<br />

vegetation polygon, ignoring the fact that radiolocations<br />

are imprecise estimates of the animal's<br />

true location (we refer to this as "naive estimator" of<br />

habitat use). Herein, we describe 2 simpler alterna-<br />

tive procedures to accommodate location error,<br />

readily implemented in a GIS. One alternative<br />

("circular error estimator") is to treat each point as<br />

the center of a circle with radius equal to the<br />

estimated average error, and assign each location<br />

to habitat types with probability proportional to<br />

area of that habitat type within the circle. Another<br />

alternative ("nonpoint estimator") is to use estimated<br />

locations solely to construct a home range<br />

contour and compare the habitat composition of<br />

the home range (rather than of individual points)

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