identification of neuroprotective genes against alpha - acumen - The ...

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CSNK1G3 Casein kinase 1, gamma-3 CHIP C-terminus of HSC70-interacting protein Daxx Death-associated protein 6 DJ-1 Oncogene DJ-1 ERV29 Surfeit 4 FBXW7 F-box and WD40 domain protein 7 GAIP G protein alpha-interacting protein GAPDH Glyceraldehyde-3-phosphate dehydrogenase GBA Glucocerebrosidase GIGYF2 GRB10-interacting GYF protein 2 GIPC GAIP C-terminus-interacting protein GPI Glucose-6-phosphate isomerase HDAC6 Histone deacetylase 6 HTRA2 HTRA serine peptidase 2 HSF1 Heat-shock factor 1 HSP70 Heat-shock protein, 70 kDa HSPC117 Hypothetical protein 117 IgG Immunoglobulin G INSR Insulin receptor LRRK2 Leucine-rich repeat kinase 2 NRB54 Nuclear RNA-binding protein, 54 kDa ix
p38 Mitogen-activated protein kinase p38 Pael receptor Parkin-associated endothelin receptor PDE9A Phosphodiesterase 9A PI3K Phosphoinositide 3-kinase PINK1 PTEN-induced putative kinase 1 PLK2 Polo-like kinase 2 PRKN Parkin PSF Polypyrimidine tract-binding protein-associated splicing factor Q82 Polyglutamine 82 containing protein RAB1A Ras-associated protein 1A RAB3A Ras-associated protein 3A RAB8A Ras-associated protein 8A RGS Regulators of G protein signaling SEC22 Secretion deficient 22 SNCA Synuclein, alpha SYVN1 Synoviolin 1 Ub Ubiquitin Ubch7 Ubiquitin-conjugating enzyme 7 Ubch8 Ubiquitin-conjugating enzyme 8 UCHL Ubiquitin C-terminal hydrolase UCHL1 Ubiquitin C-terminal hydrolase 1 x
Page 1 and 2: IDENTIFICATION OF NEUROPROTECTIVE G
Page 3 and 4: ABSTRACT Recent functional analyses
Page 5 and 6: E3 Ubiquitin ligase ER Endoplasmic
Page 7 and 8: RT-PCR Reverse transcriptase polyme
Page 9: TAG-278 Temporary assigned gene 278
Page 13 and 14: ACKNOWLEDGMENTS First and foremost,
Page 15 and 16: CONTENTS ABSTRACT .................
Page 17 and 18: a. Abstract .......................
Page 19 and 20: LIST OF FIGURES 1.1. Schematic repr
Page 21 and 22: Parkinson disease CHAPTER ONE INTRO
Page 23 and 24: al., 2006) and torsinA (Cao et al.,
Page 25 and 26: endoplasmic reticulum (ER) to Golgi
Page 27 and 28: c-Jun N-terminal kinase and caspase
Page 29 and 30: lysosomal protease promotes α-syn
Page 31 and 32: ROS, which may oxidize DA (a neurot
Page 33 and 34: together, although anti-oxidant act
Page 35 and 36: multicellular eukaryotic model orga
Page 37 and 38: C. elegans PD models C. elegans off
Page 39 and 40: Current studies To investigate gene
Page 41 and 42: Specific outcomes and future direct
Page 43 and 44: Corti, O., Hampe, C., Koutnikova, H
Page 45 and 46: Lautier, C., Goldwurm, S., Dürr, A
Page 47 and 48: Scherzer, C.R., Jensen, R.V., Gulla
Page 49 and 50: Willcox, B.J., Donlon, T.A., He, Q.
Page 51 and 52: Table 1.2. Summary of selected inve
Page 53 and 54: FIGURE LEGENDS Fig. 1.1. Schematic
Page 55 and 56: ABSTRACT Genomic multiplication of
Page 57 and 58: et al., 2003). Maintenance of DA ne
Page 59 and 60: Pdat-1::FLAG-R05D11.6, Punc-54::DsR
Page 61 and 62:
Biochemicals) and 1:10000 horseradi
Page 63 and 64:
was washed with 100 µl RNase-free
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and effectively discerned via RNAi
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protein. The primary RNAi screen of
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expressing a polyglutamine::GFP fus
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stage (Fig. 2.2B-D). Two genes exhi
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with strong expression localized to
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Our data demonstrating that the C.
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disparate datasets, such as those o
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Driscoll, M., Gerstbrein, B. (2003)
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Singleton, A.B., Farrer, M., Johnso
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Table 2.2. Bioinformatic associatio
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Table 2.4. Results of all genes kno
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C09G12.9 Tumor susceptibility gene
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C32B5.13 Cathepsin H precursor C32B
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D1014.3 Alpha-soluble NSF attachmen
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F22B7.5 DNAJA3, mitochondrial precu
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F40F4.5 Tubulin alpha-ubiquitous ch
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F54F7.5 Serine/threonine kinase 24
Page 99 and 100:
K07A9.2 CAMK1 K07C11.9 Hypothetical
Page 101 and 102:
R74.4 DNAJB1 precursor T01B7.4 Pept
Page 103 and 104:
T22D1.9 PSMD2 T22F3.2 Ubiquitin spe
Page 105 and 106:
Y38F2AL.3 Vacuolar ATP synthase sub
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Y73C8C.8 CENPE variant protein (Fra
Page 109 and 110:
Table 2.5. Summary of RNAi knockdow
Page 111 and 112:
Figure 2.2 91
Page 113 and 114:
Figure 2.4 93
Page 115 and 116:
Figure 2.6 95
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FIGURE LEGENDS Figure 2.1. RNAi kno
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came from genes that were co-expres
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ABSTRACT Multiple Parkinson disease
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vacuoles (mammalian equivalent of l
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neuroprotection when the animals we
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To investigate the genetic interact
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illustrated that α-syn overexpress
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main contributor of α-syn phosphor
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Hoozemans, J.J., van Haastert, E.S.
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Figure 3.2 115
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FIGURE LEGENDS Figure 3.1. RAB3A, R
Page 139 and 140:
CHAPTER FOUR RNA INTERFERENCE SCREE
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INTRODUCTION Central to Parkinson d
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thermotolerance (Lithgow et al., 19
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mCherry were transferred onto NGM p
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20°C for 72 hrs. The gravid adults
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pathways downstream of DAF-2 exclud
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were assayed by growing the RNAi-tr
Page 153 and 154:
while DOG treatment enhanced α-syn
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enhance α-syn misfolding in a daf-
Page 157 and 158:
REFERENCES Bar-On, P., Crews, L., K
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Lesage, S., Brice, A. (2009) Hum Mo
Page 161 and 162:
Table 4.1. Summary of genes analyze
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C12D12.2 Excitatory amino acid tran
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C47B2.2 n/a C47C12.6 Amiloride-sens
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F18A1.5 Replication protein A F18E3
Page 169 and 170:
F46E10.10 Malate dehydrogenase F46E
Page 171 and 172:
K08H10.1 Dentin sialophosphoprotein
Page 173 and 174:
T05A10.5 Cysteine-rich secretory pr
Page 175 and 176:
W03G9.6 Platelet-activating factor
Page 177 and 178:
Y58G8A.1 Acetylcholine receptor sub
Page 179 and 180:
Table 4.2. Summary of positive gene
Page 181 and 182:
Figure 4.1 161
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Figure 4.3 163
Page 185 and 186:
Figure 4.5 165
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Figure 4.7 167
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Figure 4.4. Graph illustrating the
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in yeast but not in humans. In yeas
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kinase that has also been shown to
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mir-2/mir-43/mir-250/mir-797 superf
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of djr-1.1 and djr-1.2 enhance α-s
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expensive, since they require high
Page 201 and 202:
Ambros, V. (2001) Cell 107, 823-826
Page 203 and 204:
Yi, J., Tang, X.M. (1999) Cell Res
Page 205 and 206:
Figure 5.2 185
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