- Page 1: IDENTIFICATION OF NEUROPROTECTIVE G
- Page 5 and 6: E3 Ubiquitin ligase ER Endoplasmic
- Page 7 and 8: RT-PCR Reverse transcriptase polyme
- Page 9 and 10: TAG-278 Temporary assigned gene 278
- Page 11 and 12: p38 Mitogen-activated protein kinas
- Page 13 and 14: ACKNOWLEDGMENTS First and foremost,
- Page 15 and 16: CONTENTS ABSTRACT .................
- Page 17 and 18: a. Abstract .......................
- Page 19 and 20: LIST OF FIGURES 1.1. Schematic repr
- Page 21 and 22: Parkinson disease CHAPTER ONE INTRO
- Page 23 and 24: al., 2006) and torsinA (Cao et al.,
- Page 25 and 26: endoplasmic reticulum (ER) to Golgi
- Page 27 and 28: c-Jun N-terminal kinase and caspase
- Page 29 and 30: lysosomal protease promotes α-syn
- Page 31 and 32: ROS, which may oxidize DA (a neurot
- Page 33 and 34: together, although anti-oxidant act
- Page 35 and 36: multicellular eukaryotic model orga
- Page 37 and 38: C. elegans PD models C. elegans off
- Page 39 and 40: Current studies To investigate gene
- Page 41 and 42: Specific outcomes and future direct
- Page 43 and 44: Corti, O., Hampe, C., Koutnikova, H
- Page 45 and 46: Lautier, C., Goldwurm, S., Dürr, A
- Page 47 and 48: Scherzer, C.R., Jensen, R.V., Gulla
- Page 49 and 50: Willcox, B.J., Donlon, T.A., He, Q.
- Page 51 and 52: Table 1.2. Summary of selected inve
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FIGURE LEGENDS Fig. 1.1. Schematic
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ABSTRACT Genomic multiplication of
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et al., 2003). Maintenance of DA ne
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Pdat-1::FLAG-R05D11.6, Punc-54::DsR
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Biochemicals) and 1:10000 horseradi
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was washed with 100 µl RNase-free
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and effectively discerned via RNAi
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protein. The primary RNAi screen of
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expressing a polyglutamine::GFP fus
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stage (Fig. 2.2B-D). Two genes exhi
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with strong expression localized to
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Our data demonstrating that the C.
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disparate datasets, such as those o
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Driscoll, M., Gerstbrein, B. (2003)
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Singleton, A.B., Farrer, M., Johnso
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Table 2.2. Bioinformatic associatio
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Table 2.4. Results of all genes kno
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C09G12.9 Tumor susceptibility gene
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C32B5.13 Cathepsin H precursor C32B
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D1014.3 Alpha-soluble NSF attachmen
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F22B7.5 DNAJA3, mitochondrial precu
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F40F4.5 Tubulin alpha-ubiquitous ch
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F54F7.5 Serine/threonine kinase 24
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K07A9.2 CAMK1 K07C11.9 Hypothetical
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R74.4 DNAJB1 precursor T01B7.4 Pept
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T22D1.9 PSMD2 T22F3.2 Ubiquitin spe
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Y38F2AL.3 Vacuolar ATP synthase sub
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Y73C8C.8 CENPE variant protein (Fra
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Table 2.5. Summary of RNAi knockdow
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Figure 2.2 91
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Figure 2.4 93
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Figure 2.6 95
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FIGURE LEGENDS Figure 2.1. RNAi kno
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came from genes that were co-expres
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ABSTRACT Multiple Parkinson disease
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vacuoles (mammalian equivalent of l
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neuroprotection when the animals we
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To investigate the genetic interact
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illustrated that α-syn overexpress
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main contributor of α-syn phosphor
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Hoozemans, J.J., van Haastert, E.S.
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Figure 3.2 115
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FIGURE LEGENDS Figure 3.1. RAB3A, R
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CHAPTER FOUR RNA INTERFERENCE SCREE
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INTRODUCTION Central to Parkinson d
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thermotolerance (Lithgow et al., 19
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mCherry were transferred onto NGM p
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20°C for 72 hrs. The gravid adults
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pathways downstream of DAF-2 exclud
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were assayed by growing the RNAi-tr
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while DOG treatment enhanced α-syn
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enhance α-syn misfolding in a daf-
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REFERENCES Bar-On, P., Crews, L., K
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Lesage, S., Brice, A. (2009) Hum Mo
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Table 4.1. Summary of genes analyze
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C12D12.2 Excitatory amino acid tran
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C47B2.2 n/a C47C12.6 Amiloride-sens
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F18A1.5 Replication protein A F18E3
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F46E10.10 Malate dehydrogenase F46E
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K08H10.1 Dentin sialophosphoprotein
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T05A10.5 Cysteine-rich secretory pr
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W03G9.6 Platelet-activating factor
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Y58G8A.1 Acetylcholine receptor sub
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Table 4.2. Summary of positive gene
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Figure 4.1 161
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Figure 4.3 163
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Figure 4.5 165
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Figure 4.7 167
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Figure 4.4. Graph illustrating the
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in yeast but not in humans. In yeas
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kinase that has also been shown to
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mir-2/mir-43/mir-250/mir-797 superf
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of djr-1.1 and djr-1.2 enhance α-s
- Page 199 and 200:
expensive, since they require high
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Ambros, V. (2001) Cell 107, 823-826
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Yi, J., Tang, X.M. (1999) Cell Res
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Figure 5.2 185