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PAGE 15 Quantitative proteomics and in vivo PTM analysis Mode of Action analysis of targeted drugs & biomarker identification • High-end mass spectrometry and software applications facilitate quantitative analyses of the complete proteome or protein modifications such as phosphorylation or acetylation in living cells, tissue or patient samples • Monitoring of global protein expression changes and comprehensive investigation of signaling pathways to determine the influence of drug treatment, age, disease state or mutation status on biological systems • Applications include mode of action analysis of targeted drugs and discovery of biomarkers (protein expression, phosphorylation, acetylation) for patient stratification Non-responder Responder P P P P P gene expression proliferation P P Biomarker P P P P P P gene expression proliferation P P Biomarker P
PAGE 16 Complete Proteome Analysis Large-scale, label-free quantification of proteins Recent advances in sample processing and mass spectrometry enables fast identification of ~7,000 proteins per cell line In a comparative proteomics analysis co-published by <strong>Evotec</strong> <strong>Munich</strong> scientists, more than 10,000 proteins were identified across 11 cell lines (this corresponds to 51% of the human genes) Comparisons with deep sequencing data indicate this covers most of the proteome expressed in human cancer cell lines Proteome profiling has thus reached a level of comprehensiveness similar to mRNA profiling Geiger et al, 2012,, MCP, 11, M111 014050 Schaab et al., 2012, MCP, 11, M111 014068
Page 1 and 2: Evotec Munich Building innovative d
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Page 7 and 8: Kd [µM] 0.01 0.1 1.0 10 PAGE 6 PI3
Page 9 and 10: 0,1 1 10 K d [µM] PAGE 8 Parafibro
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Page 13 and 14: 0,01 0,1 1 10 K d[µM] PAGE 12 MAP4
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Page 23 and 24: Test sample set (cell lines, xenogr
Page 25: Your contact: proteomics@evotec.com

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