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2. Thiosulfate Citerate Bile salt<br />
Sucrose agar (TCBS)<br />
• TCBS is the medium of choice for the isolation of V.<br />
<strong>cholerae</strong>.<br />
• easy to prepare, requires no autoclaving .<br />
• it has a relatively short shelf life once prepared (3 to 5<br />
days) unless plates are carefully protected against<br />
drying.<br />
• The non –inoculated m.o is olive green.<br />
• bromo thymol blue is the indicator.<br />
• V.<strong>cholerae</strong> is sucrose – fermenter.<br />
• Asid production drop the ph & change colour to yellow.
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