Vibrio cholerae

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• 2 ml of faeses + 20 ml APW ph 8-8.5 are incubated for 5 h. • At the same time the TCBS is heavily inoculated & left overnight. 3. From the TCBS,APW,an another APW are inoculated & left 5 h.
Furtheer identification of V. 1. string test: <strong>cholerae</strong>: • a drop of 0.5% Na-deoxycholate is mixed with a colony after 60 seconds with aloop there will be a thread of tenacious like nature it is +ve test for V.<strong>cholerae</strong>. 2. Poly valent antisera(anti O1,anti O139): agglutination test with specific antisera&emulsified colonies is carried out to .
Page 1 and 2: Gram negative curved bacilli • Pr
Page 3 and 4: General charectarestics: • Gram n
Page 5 and 6: • The most important species are:
Page 7 and 8: • Filippo Pacini (1812- 1883) - 1
Page 9 and 10: Vibrio cholerae • Have 2 imp anti
Page 11 and 12: Differentiation between classical a
Page 13 and 14: Biochemical characteristics of V. c
Page 15 and 16: Culture of V. cholerae 1. Alkaline
Page 17 and 18: Overnight colonies of V. cholerae o
Page 19 and 20: TTGA
Page 21 and 22: 5. Taurocholate pepton broth • Th
Page 23 and 24: MacConkey agar
Page 25: Practical procedure of cultevation
Page 29 and 30: 3. Biochemical test : • including
Page 31 and 32: • 2. Enterotoxine : it has amolec
Page 33: • The A1portion increase the intr

Vibrio cholerae

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