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ASTERICS Software manual (version 2.x, in english)

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MANUAL FOR AQEM EUROPEAN STREAM ASSESSMENT PROGRAM VERSION 2.3<br />

AQEM European stream assessment program<br />

Version 2.3, released on April 2004<br />

English Manual<br />

1


Development<br />

MANUAL FOR AQEM EUROPEAN STREAM ASSESSMENT PROGRAM VERSION 2.3<br />

Version 1.0 of this program (released <strong>in</strong> March 2002) was developed <strong>in</strong> the EU<br />

funded project:<br />

„The Development and Test<strong>in</strong>g of an Integrated Assessment System for the<br />

Ecological Quality of Streams and Rivers throughout Europe us<strong>in</strong>g Benthic<br />

Macro<strong>in</strong>vertebrates“<br />

A project under the 5th European Framework Program<br />

Energy, Environment and Susta<strong>in</strong>able Development<br />

Key Action 1: Susta<strong>in</strong>able Management and Quality of Water<br />

Contract No: EVK1-CT1999-00027<br />

Calculation methods developed by the AQEM Consortium.<br />

<strong>Software</strong> was programmed by:<br />

Wagen<strong>in</strong>gen <strong>Software</strong> Labs<br />

P.O.Box 47<br />

6700 AA Wagen<strong>in</strong>gen<br />

The Netherlands<br />

http://www.wisl.nl<br />

The taxalist used for the calculations was generated by the AQEM consortium. The<br />

autecological <strong>in</strong>formation (e.g. feed<strong>in</strong>g types, habitat preferences) are predom<strong>in</strong>antly<br />

taken from:<br />

1. Moog, O. (Ed.) (1995): Fauna Aquatica Austriaca - a comprehensive species <strong>in</strong>ventory<br />

of Austrian aquatic organisms with ecological data.- First edition,<br />

Wasserwirtschaftskataster, Bundesm<strong>in</strong>isterium für Land- und Forstwirtschaft,<br />

Wien.<br />

2. Schmedtje, U. and M. Coll<strong>in</strong>g (1996): Ökologische Typisierung der aquatischen<br />

Makrofauna.-Informationsberichte des Bayerischen Landesamtes für<br />

Wasserwirtschaft 4/96.<br />

3. Information gathered by the AQEM consortium.<br />

Update to Version 2.0<br />

Enhancements <strong>in</strong> Version 2.0 refer especially to the results of the project<br />

„Leitbildorientierte biologische Fließgewässerbewertung zur Charakterisierung des<br />

Sauerstoffhaushaltes“ (funded by the German Federal Environment Agency<br />

(Umweltbundesamt)), Förderkennzeichen UFOPLAN 200 24 227).<br />

Essential parts of the update are:<br />

• Version 1.0 <strong>in</strong>cluded only five of the at that time specified 20 German stream types. With<br />

this update the rema<strong>in</strong><strong>in</strong>g types have been <strong>in</strong>tegrated and the calculation of a typespecific<br />

saprobic <strong>in</strong>dex was enabled (module “organic pollution”).<br />

• A new metric (Potamon Typie Index) was added, an <strong>in</strong>dex with relevance for future<br />

assessment of rivers <strong>in</strong> Germany.<br />

2


MANUAL FOR AQEM EUROPEAN STREAM ASSESSMENT PROGRAM VERSION 2.3<br />

• In addition to Version 1.0 with only an English user <strong>in</strong>terface this <strong>version</strong> was<br />

programmed with an additional German user <strong>in</strong>terface to elim<strong>in</strong>ate problems with different<br />

formats (e.g. decimal po<strong>in</strong>ts) that lead to malfunction of the program.<br />

• The helpfile was updated and a German helpfile was added.<br />

Update to Version 2.3 (released <strong>in</strong> April 2004)<br />

Enhancements <strong>in</strong> Version 2.3 refer to a large amount to the project<br />

„Weiterentwicklung und Anpassung des nationalen Bewertungssystems für<br />

Makrozoobenthos an neue <strong>in</strong>ternationale Vorgaben“ (funded by the German Federal<br />

Environment Agency (Umweltbundesamt)), Förderkennzeichen UFOPLAN 202 24<br />

223).<br />

Essential parts of the update are:<br />

• Version 2.3 discrim<strong>in</strong>ates two different assessment systems for Germany, the AQEM<br />

orig<strong>in</strong>al system and the German official system.<br />

• With the orig<strong>in</strong>ally developed system ( „Germany, orig<strong>in</strong>al AQEM“) the impairment of<br />

macro<strong>in</strong>vertebrates caused by the stressor „Degradation <strong>in</strong> stream morphology“ can be<br />

detected for five of the German stream types (types 5, 9, 11, 14, 15). This system was<br />

already implemented <strong>in</strong> <strong>version</strong> 1.0.<br />

• The new system (“Germany, official system”) was developed as a multimetric, module<br />

based assessment system for (almost) all stream types <strong>in</strong> Germany. The streamtypes are<br />

specified accord<strong>in</strong>g to “Gewässertypentabelle der Länderarbeitsgeme<strong>in</strong>schaft Wasser<br />

(LAWA) (Sommerhäuser & Pottgiesser 2003, nach Schmedtje et al. 2001)“. Assessment<br />

of miss<strong>in</strong>g types (types 22 and 23) will be available by the end of 2004. For some types<br />

not all modules can be used (e.g. types 4 and 21: only “organic pollution”).<br />

• The “Germany, official system” <strong>in</strong>cludes three modules and was designed stressorspecific.<br />

Start<strong>in</strong>g with a taxa list <strong>in</strong>formation on general degradation (all stream types<br />

except types 4 and 21), saprobic condition (all stream types) and acidification (stream<br />

types 5 and 5.1) can be gathered and a “Leitbild”-based assessment can be made.<br />

• Additional metrics have been added (for assessment of German stream types).<br />

• The taxa database was updated (e.g. new autecological <strong>in</strong>formation was added).<br />

Installation<br />

The AQEM software is available on www.aqem.de or on the website of the<br />

Department of Hydrobiology, University of Duisburg-Essen (http://www.uniessen.de/hydrobiologie/g_frameseite_limnodirect.htm).<br />

To <strong>in</strong>stall the program you have to<br />

start “Setup.exe”. The <strong>in</strong>stall shield will automatically lead through the <strong>in</strong>stallation<br />

process. Due to technical reasons a standard <strong>in</strong>stallation path is used. It is essential<br />

to have adm<strong>in</strong>istrator access for your computer because otherwise the <strong>in</strong>stallation<br />

can not be completed or the program will not work <strong>in</strong> the proper way.<br />

3


System requirements<br />

MANUAL FOR AQEM EUROPEAN STREAM ASSESSMENT PROGRAM VERSION 2.3<br />

To be able to run the AQEM program the follow<strong>in</strong>g system requirements have to be<br />

met:<br />

• Microsoft operat<strong>in</strong>g system W<strong>in</strong>dows 98, 2000, NT 4; W<strong>in</strong>dows 2000 or<br />

W<strong>in</strong>dows XP<br />

• Microsoft Office 97, 2000 or XP has to be <strong>in</strong>stalled on the computer for the<br />

read<strong>in</strong>g and writ<strong>in</strong>g of data <strong>in</strong>put and output files (EXCEL) and establish<strong>in</strong>g<br />

connections with the ACCESS databases.<br />

The AQEM software is designed for English and German <strong>version</strong>s of Microsoft<br />

W<strong>in</strong>dows and Microsoft Office. When runn<strong>in</strong>g the AQEM software EXCEL must be<br />

closed.<br />

Support<br />

Technical support and specific <strong>in</strong>formation on the AQEM assessment software may<br />

be addressed to University of Duisburg-Essen (aqem@uni-essen.de).<br />

Questions referr<strong>in</strong>g to the taxa database may be addressed to Arm<strong>in</strong> Lorenz,<br />

Universität Duisburg-Essen (arm<strong>in</strong>.lorenz@uni-essen.de).<br />

4


1 Introduction<br />

MANUAL FOR AQEM EUROPEAN STREAM ASSESSMENT PROGRAM VERSION 2.3<br />

The AQEM European stream assessment program (Versions 2.0 und 2.3) was<br />

designed to assess the ecological quality of streams and rivers throughout Europe <strong>in</strong><br />

compliance with the European Water Framework Directive us<strong>in</strong>g macro<strong>in</strong>vertrebrates.<br />

For a total of 48 European stream types distributed among Germany, Greece, Italy,<br />

the Netherlands, Austria, Portugal, Sweden, and Czech Republic an assessment<br />

based on macro<strong>in</strong>vertebrate taxalists can be made with this program giv<strong>in</strong>g the<br />

follow<strong>in</strong>g <strong>in</strong>formation:<br />

• Ecological Quality Class, derived from a number of stream type-specific metrics<br />

that are strongly correlated with the degradation of a stream (metrics correspond<br />

to a specific stressor, e.g. organic pollution, morphological degradation)<br />

• A large number of additional metrics which are helpful for further <strong>in</strong>terpretation of<br />

the data.<br />

The AQEM software is only a calculation program and not designed for stor<strong>in</strong>g data.<br />

The reason beh<strong>in</strong>d this limitation lies <strong>in</strong> the multitude of databases which are<br />

presently used by different European water authorities. It can be expected and it is<br />

reasonable that the authorities will cont<strong>in</strong>ue to use those databases <strong>in</strong> the future. The<br />

AQEM software is based on EXCEL as common and comparatively compatible<br />

comput<strong>in</strong>g system, to which most databases are able to export data sheets.<br />

The AQEM assessment system was developed under specific conditions that have to<br />

be met when us<strong>in</strong>g this program to guarantee that the results are validated. The<br />

system is based on a certa<strong>in</strong> sampl<strong>in</strong>g procedure for macro<strong>in</strong>vertebrates (multihabitat<br />

sampl<strong>in</strong>g) and the taxonomic adjustment of the result<strong>in</strong>g taxa list. This<br />

procedure is described <strong>in</strong> detail <strong>in</strong> the AQEM <strong>manual</strong> that can be found on the<br />

website of the AQEM project (products) (http://www.aqem.de/).<br />

5


MANUAL FOR AQEM EUROPEAN STREAM ASSESSMENT PROGRAM VERSION 2.3<br />

2 Application of the AQEM program<br />

2.1 Start W<strong>in</strong>dow<br />

By choos<strong>in</strong>g one of the flags the correspond<strong>in</strong>g <strong>in</strong>formation for the selected country is<br />

loaded <strong>in</strong>to the program and the ma<strong>in</strong> program w<strong>in</strong>dow is opened. The country<br />

selection can be changed later <strong>in</strong> the ma<strong>in</strong> program w<strong>in</strong>dow or <strong>in</strong> the sample<br />

characterisation w<strong>in</strong>dow.<br />

FIGURE 1: Start W<strong>in</strong>dow of the AQEM software.<br />

2.2 Ma<strong>in</strong> program W<strong>in</strong>dow<br />

Figure 2 shows the Ma<strong>in</strong> Program W<strong>in</strong>dow of the AQEM software. The country<br />

selected for exemplary display is the Netherlands as shown <strong>in</strong> the drop-down box on<br />

the top left. In this drop-down list other countries can be selected.<br />

6


MANUAL FOR AQEM EUROPEAN STREAM ASSESSMENT PROGRAM VERSION 2.3<br />

There are three buttons <strong>in</strong> the upper right-hand frame of the w<strong>in</strong>dow:<br />

• The “Help” button opens the help function.<br />

• The “Info” button opens a box display<strong>in</strong>g general <strong>in</strong>formation about the program,<br />

<strong>in</strong>formation about the consortium and the sources of autecological <strong>in</strong>formation.<br />

• The “Exit” button closes the program.<br />

FIGURE 2: Ma<strong>in</strong> Program W<strong>in</strong>dow of the AQEM software (before import of a taxa list).<br />

To run the calculations a taxa list generated from the sampl<strong>in</strong>g site to be assessed<br />

must first be imported.<br />

Note! Save the list before import<strong>in</strong>g it <strong>in</strong>to the AQEM software, as the AQEM<br />

software will alter the contents of the file. When import<strong>in</strong>g a taxalist EXCEL must be<br />

closed.<br />

2.2.1 Import<strong>in</strong>g taxa lists<br />

The “Import” button opens a standard w<strong>in</strong>dows dialog box, from which the taxa list to<br />

be imported can be selected.<br />

The import file can either be an Excel or an ASCII table. Tables <strong>in</strong> either format must<br />

be layouted correctly (see section “File layout for import<strong>in</strong>g taxa lists”). Otherwise the<br />

AQEM software is unable to import the data correctly.<br />

7


MANUAL FOR AQEM EUROPEAN STREAM ASSESSMENT PROGRAM VERSION 2.3<br />

After the import file is selected the follow<strong>in</strong>g dialog box appears:<br />

FIGURE 3: Dialog box for selection of Key Code for import<strong>in</strong>g taxa lists<br />

When import<strong>in</strong>g taxa lists to computer programs one problem which often occurs is<br />

that taxa are <strong>in</strong>correctly recognised and falsely imported <strong>in</strong>to the program. Different<br />

use of nomenclature, different abbreviations (e.g. “spec.” or “sp.”) or simple spell<strong>in</strong>g<br />

mistakes can lead to errors and confusion. Therefore, most systems use<br />

identification codes to clearly identify taxa.<br />

S<strong>in</strong>ce the AQEM system is designed for application <strong>in</strong> several countries, the AQEM<br />

software offers four possibilities to specify taxa. One common identifier must be<br />

selected <strong>in</strong> the “Import file sett<strong>in</strong>gs” dialog box as the import “Key Value”.<br />

Valid Key Values are:<br />

Column head<strong>in</strong>g <strong>in</strong> file Description<br />

Shortcode Taxa shortcode for <strong>in</strong>ternal use <strong>in</strong> the AQEM project<br />

ID_ART Taxa identification number for <strong>in</strong>ternal use <strong>in</strong> the AQEM<br />

project; coherent to the Austrian standard taxa identification<br />

code, used <strong>in</strong> the Austrian software ECOPROF<br />

DINNo German DV-Code: the German standard taxa<br />

identification code<br />

TAXON_NAME Taxon name<br />

The import file table should conta<strong>in</strong> an extra column for one of the Key Value codes<br />

listed above. If the table does not conta<strong>in</strong> any of the other codes it must be imported<br />

us<strong>in</strong>g “TAXON_NAME” as the Key Value.<br />

Note! Head<strong>in</strong>gs <strong>in</strong> the Key Value columns <strong>in</strong> the import file table must be written <strong>in</strong><br />

exactly the same way as they are written <strong>in</strong> the table above to ensure correct import<br />

of the data.<br />

After select<strong>in</strong>g a Key Value from the “Import file sett<strong>in</strong>gs” dialog box, press OK to<br />

import the selected file. Once the list is imported the program automatically l<strong>in</strong>ks<br />

each imported taxon with the relevant autecological <strong>in</strong>formation from the taxa<br />

database accord<strong>in</strong>g to the Key Value.<br />

8


2.2.1.1 Save file<br />

MANUAL FOR AQEM EUROPEAN STREAM ASSESSMENT PROGRAM VERSION 2.3<br />

To save the imported taxa list, press the “Save file” button. If a file with the same file<br />

name already exists the program prompts a warn<strong>in</strong>g, and gives the possibility to<br />

change the filename and/or file type.<br />

The file can either be saved as an EXCEL datasheet or as an ASCII text file <strong>in</strong> the<br />

format described <strong>in</strong> section “File Layout for import<strong>in</strong>g a taxa list”. The option to save<br />

the file as an EXCEL datasheet is only given, when EXCEL is <strong>in</strong>stalled on the<br />

computer where the AQEM program is be<strong>in</strong>g used.<br />

2.2.1.2 Save file as<br />

The “Save file as” button opens a standard w<strong>in</strong>dows dialog box, where it is possible<br />

to select the name and file type, to which the imported taxa list will be saved.<br />

Two file types are possible, a pla<strong>in</strong>-text type (ASCII) and an Excel datasheet. The<br />

option to save the file as an EXCEL datasheet is only given, when EXCEL is <strong>in</strong>stalled<br />

on the computer where the AQEM program is be<strong>in</strong>g used.<br />

2.2.2 File layout for import<strong>in</strong>g a taxa list<br />

2.2.2.1 EXCEL-file<br />

Figure 4 shows an example how an EXCEL sheet must be formatted and layouted <strong>in</strong><br />

order to be imported successfully.<br />

FIGURE 4: Example of an EXCEL <strong>in</strong>put data sheet for import <strong>in</strong>to the AQEM software.<br />

9


MANUAL FOR AQEM EUROPEAN STREAM ASSESSMENT PROGRAM VERSION 2.3<br />

The first column must conta<strong>in</strong> the Key Value. The second column must conta<strong>in</strong> the<br />

taxa names. The third and follow<strong>in</strong>g columns conta<strong>in</strong> the abundances of the species<br />

<strong>in</strong> each sample; a “0” <strong>in</strong>dicates that this particular taxon is not present <strong>in</strong> the sample.<br />

The abundances should be given <strong>in</strong> either “<strong>in</strong>dividuals/m 2 ” (Czech Republic,<br />

Germany), “<strong>in</strong>dividuals/1.25 m 2 ” (Austria, Greece, Portugal, The Netherlands,<br />

Sweden), “<strong>in</strong>dividuals/0.5 m 2 ” (Italian stream types I02 and I03) or “<strong>in</strong>dividuals/0.8 m 2 ”<br />

(Italian stream type I04).<br />

Note that cells A1 and B1 and the text they conta<strong>in</strong> must be formatted exactly the<br />

way given <strong>in</strong> Figure 4. Instead of “ID_ART”, cell A1 may also conta<strong>in</strong> “DINNo”,<br />

“Shortcode”, “TAXON_NAME”, depend<strong>in</strong>g on which Key Value the user plans to use<br />

for import<strong>in</strong>g the file.<br />

When the imported sample file is saved it will have the same layout as given <strong>in</strong><br />

Figure 4.<br />

2.2.2.2 ASCII-file<br />

The program is also capable of import<strong>in</strong>g a pla<strong>in</strong> ASCII file, which should be<br />

arranged as shown <strong>in</strong> Figure 5. Use “;” to separate the columns <strong>in</strong> the ASCII-file.<br />

FIGURE 5: Example of an ASCII <strong>in</strong>put file.<br />

The first column conta<strong>in</strong>s the Key Value, the second column conta<strong>in</strong>s the taxa<br />

names. The third and follow<strong>in</strong>g columns conta<strong>in</strong> the abundances (Ind/m²) of the<br />

species <strong>in</strong> each sample; a “0” <strong>in</strong>dicates that this particular taxa is not present <strong>in</strong> the<br />

sample.<br />

When the imported sample file is saved it will have the same layout as given <strong>in</strong><br />

Figure 5.<br />

10


MANUAL FOR AQEM EUROPEAN STREAM ASSESSMENT PROGRAM VERSION 2.3<br />

2.3 Automatic replacement of taxa<br />

The taxa list is imported by compar<strong>in</strong>g the selected Key Value with the respective<br />

Key Values from the taxa database, which is part of the program. If the Key<br />

Value/taxon name comb<strong>in</strong>ation <strong>in</strong> the imported taxa list differ from the Key<br />

Value/taxon name comb<strong>in</strong>ation <strong>in</strong> the database, the taxon name will automatically be<br />

replaced.<br />

If taxa have been replaced, a list of these taxa is displayed <strong>in</strong> the Replaced Taxa<br />

Names W<strong>in</strong>dow (Figure 6). It is possible to save this list as an EXCEL file.<br />

Note! If a taxon name does not correspond the Key Value the program will<br />

automatically replace the taxon name by the taxon name belong<strong>in</strong>g to the Key Value.<br />

Check the Replaced Taxa Names W<strong>in</strong>dow always carefully to avoid <strong>in</strong>correct<br />

changes result<strong>in</strong>g from mistyped Key Values <strong>in</strong> the <strong>in</strong>put file.<br />

FIGURE 6: List of automatically replaced taxa<br />

11


MANUAL FOR AQEM EUROPEAN STREAM ASSESSMENT PROGRAM VERSION 2.3<br />

2.4 Replacement of unknown taxa<br />

If a taxa cannot be found or correctly merged with<strong>in</strong> the database, it can be altered<br />

<strong>manual</strong>ly. These “unknown” taxa are displayed <strong>in</strong> the Replace Unknown Taxa<br />

W<strong>in</strong>dow (Figure 7). In this w<strong>in</strong>dow, these taxa can either be replaced by another<br />

taxon or deleted from the imported taxa list.<br />

Figure 7: List of unknown taxa from an imported taxa list. The first column shows the<br />

unknown taxon ID (Key Value) from the import file; the second column shows the<br />

unknown taxon name from the import file; the third column shows the action to be<br />

taken (Note! The default sett<strong>in</strong>g is: removal of the taxon); the fourth column shows<br />

the new taxon name if the taxon is replaced.<br />

The user can select the unknown taxon and search for an alternative name <strong>in</strong> the<br />

drop-down list. The taxon can be replaced by press<strong>in</strong>g the “Replace by” button. In<br />

this case the “Action” column will show “”.<br />

If the user wants to delete the taxon he can select the taxon and press the “Delete”<br />

button. In this case the “Action” column will show “”. Note that default sett<strong>in</strong>g<br />

will delete the taxon; there is no need to specifically delete the taxon by select<strong>in</strong>g it<br />

and press<strong>in</strong>g the “Delete” button.<br />

The “OK” button will confirm the actions selected for all taxa <strong>in</strong> the list, and will start<br />

perform<strong>in</strong>g the correspond<strong>in</strong>g changes <strong>in</strong> the sample taxa lists. Unknown taxa<br />

marked as <strong>in</strong> the ”Action” column, will be removed from the imported taxa<br />

list. Taxa marked as <strong>in</strong> the “Action” column, will be replaced with the<br />

selected taxon name from column “New taxon name” and the correspond<strong>in</strong>g Key<br />

12


MANUAL FOR AQEM EUROPEAN STREAM ASSESSMENT PROGRAM VERSION 2.3<br />

Value from the database will be updated <strong>in</strong> the import taxa list. If the new taxon<br />

already exists <strong>in</strong> the sample list, the abundances will be added to the exist<strong>in</strong>g entry<br />

for the taxon.<br />

The “Cancel” button cancels all the actions and returns to the Ma<strong>in</strong> Program W<strong>in</strong>dow<br />

without import<strong>in</strong>g the taxa list <strong>in</strong>to the program.<br />

Note! Press<strong>in</strong>g the OK button causes all action to be undertaken simultaneously. Be<br />

sure that all the actions <strong>in</strong> the “Action“ column of Replace Unknown Taxa W<strong>in</strong>dow<br />

are set as you wish before press<strong>in</strong>g the OK button.<br />

2.5 Sample Characterisation<br />

Once all the taxa are successfully imported <strong>in</strong>to the program the Sample<br />

Characterisation W<strong>in</strong>dow is automatically shown (Figure 6).<br />

Figure 8: The Sample Characterisation W<strong>in</strong>dow.<br />

In the example given <strong>in</strong> Figure 8, taxa lists from five sampl<strong>in</strong>g sites have been<br />

imported <strong>in</strong>to the sample file. For every list a country, a stream type and a stressor<br />

must be selected. See Section 2.8 “Valid stream type/stressor comb<strong>in</strong>ations” for a<br />

detailed table of valid comb<strong>in</strong>ations.<br />

13


MANUAL FOR AQEM EUROPEAN STREAM ASSESSMENT PROGRAM VERSION 2.3<br />

The country is by default set to the country chosen when the program was started.<br />

The default stream type and stressor are the first stream type/stressor comb<strong>in</strong>ation <strong>in</strong><br />

the list given <strong>in</strong> Table 1 (Chapter 2.8). Other stream types and stressors can be<br />

chosen <strong>in</strong> the drop-down list.<br />

Country, stream type and/or stressor can be changed either for all samples at once<br />

to the same stream type and stressor or <strong>in</strong>dividually for each sample:<br />

1. In the top frame of the w<strong>in</strong>dow there are three drop-down boxes for select<strong>in</strong>g the<br />

appropriate characters. If the check-boxes above the drop-down boxes are<br />

checked then press<strong>in</strong>g the “Apply to all” button will apply the selected characters<br />

to all samples <strong>in</strong> the list.<br />

2. By double-click<strong>in</strong>g on any given cell, which is to be changed, a drop-down list<br />

with the possible characters will appear. See also Figure 9 for an example, where<br />

the list with stream types is shown for an <strong>in</strong>dividual sample.<br />

FIGURE 9: Drop-down list for select<strong>in</strong>g a stream type for an <strong>in</strong>dividual sample.<br />

Any selected sample can also be copied, e.g. <strong>in</strong> case two stressors need to be<br />

calculated for the same sample. This should always be done if two stressors are<br />

“valid” for a certa<strong>in</strong> stream type (chapter 2.8, Table 1). If two stressors have been<br />

selected, the sample will be mentioned twice <strong>in</strong> the Summary W<strong>in</strong>dow (e.g. result 1 =<br />

Quality Class for “organic pollution”; result 2 = Quality Class for “degradation <strong>in</strong><br />

stream morphology”). In the present <strong>version</strong> of the AQEM software the “f<strong>in</strong>al”<br />

Ecological Quality Class needs to be calculated “by hand” us<strong>in</strong>g the worst case out of<br />

the two stressor-specific results.<br />

14


MANUAL FOR AQEM EUROPEAN STREAM ASSESSMENT PROGRAM VERSION 2.3<br />

To be copied a sample has first to be marked and then the “Copy” button has to be<br />

pressed. The name of a newly generated sample by copy<strong>in</strong>g can be edited by double<br />

click<strong>in</strong>g on the sample name. Any selected sample can also be deleted from the list.<br />

• The “Export to Excel” button saves the Sample Characterisation table as an<br />

Excel file.<br />

• The “Cancel” button discards all changes made and returns to the Ma<strong>in</strong> Program<br />

W<strong>in</strong>dow.<br />

• The “OK” button confirms all the changes and returns to the Ma<strong>in</strong> Program<br />

W<strong>in</strong>dow.<br />

• The “Help” button prompts this text.<br />

2.6 Ma<strong>in</strong> program W<strong>in</strong>dow after a successful taxa list import<br />

Once all imported taxa have been recognised and the stream type and stressor for<br />

the samples have been selected the Ma<strong>in</strong> Program W<strong>in</strong>dow appears as shown <strong>in</strong><br />

Figure 8. Note that the buttons “Save”, “Save as”, “Samples Char.”, “Calculate” and<br />

“Autecological Info”, which were grey before the taxa list was imported are now<br />

functional.<br />

FIGURE 10: Ma<strong>in</strong> screen with an imported taxa list.<br />

15


MANUAL FOR AQEM EUROPEAN STREAM ASSESSMENT PROGRAM VERSION 2.3<br />

The first column displays the Key Value applied for import<strong>in</strong>g the data. The second<br />

column conta<strong>in</strong>s the taxon name, the third column the shortcode. The follow<strong>in</strong>g<br />

columns display the abundance of each taxon <strong>in</strong> the <strong>in</strong>dividual samples.<br />

• The “Save” button saves the imported samples with the current file name.<br />

• The “Save as” button saves the imported samples with a user-def<strong>in</strong>ed file name;<br />

also the file type can be chosen (EXCEL or pla<strong>in</strong> ASCII).<br />

• The “Sample char.” button opens the Sample Characterisation W<strong>in</strong>dow where<br />

the stream types and stressors can be selected. Note that only a limited<br />

comb<strong>in</strong>ation of stream types and stressors are valid. See chapter 2.8 “Valid<br />

stream type/stressor comb<strong>in</strong>ations”.<br />

• The “Autecological <strong>in</strong>formation” button opens a large data sheet, where all the<br />

scores are given for each taxon <strong>in</strong> the sample list, which are used for calculat<strong>in</strong>g<br />

the metrics. See section 2.9 “Autecological Information W<strong>in</strong>dow” for detailed<br />

<strong>in</strong>formation.<br />

• The “Calculate” button starts the calculation for the samples depend<strong>in</strong>g on the<br />

selected stream type and stressor. See section 2.7 “Calculation Results” for<br />

more <strong>in</strong>formation.<br />

2.7 Calculation Results<br />

The calculation results are summarised <strong>in</strong> the Sample Score W<strong>in</strong>dow <strong>in</strong> two separate<br />

sheets: “Summary” and “Metrics”. It is possible to save both screens separately as<br />

EXCEL files.<br />

2.7.1 Summary sheet<br />

The “Summary” sheet which automatically appears when the “Sample score” W<strong>in</strong>dow<br />

is opened displays the results of those metrics used to calculate the Ecological<br />

Quality Class for the selected stream type and stressor. The calculated Ecological<br />

Quality Class is also displayed.<br />

Results are only displayed, if a valid stream type/stressor comb<strong>in</strong>ation has been<br />

chosen <strong>in</strong> the “Sample Characterisation” W<strong>in</strong>dow. Otherwise the screen displays no<br />

values.<br />

16


MANUAL FOR AQEM EUROPEAN STREAM ASSESSMENT PROGRAM VERSION 2.3<br />

FIGURE 11: The Sample Scores W<strong>in</strong>dow Summary sheet.<br />

The Ecological Quality Class of the sample is given <strong>in</strong> the row “results” and can be<br />

either:<br />

5 (high) (<strong>in</strong>dicated by a blue colour <strong>in</strong> the “Results/Quality class” l<strong>in</strong>e)<br />

4 (good) (<strong>in</strong>dicated by a green colour)<br />

3 (moderate) (<strong>in</strong>dicated by a yellow colour)<br />

2 (poor) (<strong>in</strong>dicated by a orange colour)<br />

1 (bad) (<strong>in</strong>dicated by a red colour)<br />

The results for all the calculated metrics are given for each sample. The first column<br />

shows the metric name, the second column the result of the metric and the third<br />

column the respective “Quality Class” of the metric rang<strong>in</strong>g from 5 (high) to 1 (bad).<br />

If the metric does not qualify for calculat<strong>in</strong>g the Ecological Quality Class because the<br />

value is out of range or certa<strong>in</strong> organism groups have not been sampled, which are<br />

needed to calculate a certa<strong>in</strong> metric or no transformation to a quality class was<br />

made “-“ is given <strong>in</strong> the third column.<br />

2.7.2 Metrics sheet<br />

The Metrics sheet shows the results of all metrics calculated by the program. Most of<br />

the metrics are not used to calculate the Ecological Quality Class but are helpful for<br />

data <strong>in</strong>terpretation.<br />

The metrics are expla<strong>in</strong>ed <strong>in</strong> detail <strong>in</strong> Annex 9 of the AQEM <strong>manual</strong> (see<br />

www.aqem.de → products).<br />

17


MANUAL FOR AQEM EUROPEAN STREAM ASSESSMENT PROGRAM VERSION 2.3<br />

FIGURE 12: The Sample scores W<strong>in</strong>dow Metrics sheet. “Not calculated” <strong>in</strong>dicates that a<br />

certa<strong>in</strong> metric has not been calculated s<strong>in</strong>ce taxa relevant for the calculation<br />

have not been recorded.<br />

2.8 Valid stream type/stressor comb<strong>in</strong>ations<br />

Table 1 shows the valid comb<strong>in</strong>ations of stream type and stressors, which can be<br />

used for calculation of data with the orig<strong>in</strong>al AQEM system. These can be chosen <strong>in</strong><br />

the Sample Characterisation W<strong>in</strong>dow.<br />

Table 1: Valid stream type / stressor comb<strong>in</strong>ations for the orig<strong>in</strong>al AQEM system<br />

Country Stream type<br />

Sweden Small lowland streams <strong>in</strong> Northern Sweden<br />

Small mid-altitude streams <strong>in</strong> Northern Sweden<br />

Small mid-altitude streams <strong>in</strong> Boreal highlands<br />

General Degradation<br />

Degradation <strong>in</strong> Stream<br />

Morphology<br />

Stressor<br />

Organic Pollution<br />

Acidification<br />

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MANUAL FOR AQEM EUROPEAN STREAM ASSESSMENT PROGRAM VERSION 2.3<br />

Country Stream type<br />

Germany,<br />

orig<strong>in</strong>al AQEM<br />

Small high-altitude streams <strong>in</strong> Boreal highlands<br />

Mid-sized lowland streams <strong>in</strong> South Swedish<br />

Lowlands<br />

Small sand bottom streams <strong>in</strong> the German<br />

lowlands<br />

Organic type brook <strong>in</strong> the German lowlands<br />

Mid-sized sand bottom streams <strong>in</strong> the German<br />

lowlands<br />

Small streams <strong>in</strong> lower mounta<strong>in</strong>ous areas of<br />

Central Europe<br />

Mid-sized streams <strong>in</strong> lower mounta<strong>in</strong>ous areas<br />

of Central Europe<br />

The Netherlands Small Dutch lowland streams<br />

Small Dutch hill streams<br />

Czech Republic Mid-sized streams <strong>in</strong> eastern lower mounta<strong>in</strong>ous<br />

areas of Central Europe<br />

Small streams <strong>in</strong> lower mounta<strong>in</strong>ous areas of the<br />

Carpathian area<br />

Mid-sized streams <strong>in</strong> lower mounta<strong>in</strong>ous areas<br />

of the Carpathian area<br />

Austria Mid-sized streams <strong>in</strong> the Bohemian Massif<br />

Small non-glaciated crystall<strong>in</strong>e alp<strong>in</strong>e streams<br />

Mid-sized calcareous pre-alp<strong>in</strong>e streams<br />

Mid-sized streams <strong>in</strong> Hungarian Pla<strong>in</strong>s<br />

Italy Small streams <strong>in</strong> the Southern Silicate Alps<br />

Small-sized, calcareous streams, 200-800m<br />

(South Apenn<strong>in</strong>es)<br />

Mid-sized, calcareous streams, 200-800m (North<br />

Apenn<strong>in</strong>es)<br />

Small streams <strong>in</strong> the lowlands of the Po valley<br />

Portugal Small streams <strong>in</strong> lower mounta<strong>in</strong>ous areas of<br />

Southern Portugal<br />

Small lowland streams of Southern Portugal<br />

Medium-sized lowland streams of Southern<br />

Portugal<br />

General Degradation<br />

Degradation <strong>in</strong> Stream<br />

Morphology<br />

Stressor<br />

Organic Pollution<br />

Acidification<br />

19


MANUAL FOR AQEM EUROPEAN STREAM ASSESSMENT PROGRAM VERSION 2.3<br />

Country Stream type<br />

Greece Mid-sized calcareous streams <strong>in</strong> Western<br />

Greece<br />

Mid-sized high-altitude streams <strong>in</strong> North-Eastern<br />

Greece<br />

Mid-sized high-altitude streams <strong>in</strong> Central and<br />

North Greece<br />

Small streams <strong>in</strong> lower mounta<strong>in</strong>ous areas of<br />

Central Europe<br />

General Degradation<br />

Degradation <strong>in</strong> Stream<br />

Morphology<br />

Stressor<br />

Table 2 gives an overview on valid stream type / stressor comb<strong>in</strong>ations for sites to be<br />

calculated with the „Germany, official system“. These can be chosen <strong>in</strong> the Sample<br />

Characterisation W<strong>in</strong>dow.<br />

Table 2: Valid stream type / stressor comb<strong>in</strong>ations for „Germany, official system”<br />

Stream type<br />

Typ 1: Alp<strong>in</strong>e streams<br />

Typ 2: Streams <strong>in</strong> the alp<strong>in</strong>e foothills<br />

Typ 3: Streams <strong>in</strong> the pleistocene sediments of the alp<strong>in</strong>e foothills<br />

Typ 4: Large streams <strong>in</strong> the alp<strong>in</strong>e foothills<br />

Typ 5: Small siliceous cobble-bottom streams <strong>in</strong> lower-mounta<strong>in</strong>ous<br />

areas<br />

Typ 5.1: Small siliceous sandstone streams<br />

Typ 6: Small loam / sand-bottom streams (dom<strong>in</strong>ated by f<strong>in</strong>e<br />

sediments) <strong>in</strong> calcareous lower-mounta<strong>in</strong>ous areas<br />

Typ 7: Small cobble-bottom streams <strong>in</strong> calcareous lower-mounta<strong>in</strong>ous<br />

areas<br />

Typ 9: Mid-sized siliceous cobble / boulder bottom streams <strong>in</strong> lowermounta<strong>in</strong>ous<br />

areas<br />

Typ 9.1: Mid-sized streams <strong>in</strong> calcareous lower-mounta<strong>in</strong>ous areas<br />

(different substrate)<br />

General<br />

Degradation<br />

Organic Pollution<br />

Stressor<br />

Organic<br />

Pollution<br />

Acidification<br />

Acidification<br />

20


Stream type<br />

MANUAL FOR AQEM EUROPEAN STREAM ASSESSMENT PROGRAM VERSION 2.3<br />

Typ 9.2: Large cobble / boulder bottom streams <strong>in</strong> lower-mounta<strong>in</strong>ous<br />

areas<br />

Typ 10: Large gravel-bottom rivers<br />

Typ 11: Small streams with organic substrates<br />

Typ 12: Mid-sized streams with organic substrates<br />

Typ 14: Small sand-bottom streams <strong>in</strong> the lowlands<br />

Typ 15: Mid-sized sand-bottom streams <strong>in</strong> lowland<br />

Typ 15_groß: large sand-bottom streams <strong>in</strong> lowland, >1.000 km² EZG<br />

Typ 16: Small gravel-bottom streams <strong>in</strong> the lowlands<br />

Typ 17: Mid-sized to large gravel-bottom streams <strong>in</strong> the lowlands<br />

Typ 18: Loess-loam-bottom lowland streams<br />

Typ 19: Small streams <strong>in</strong> floodpla<strong>in</strong>s<br />

Typ 20: Large sand-bottom rivers<br />

Typ 21: : Streams <strong>in</strong>fluenced by lake-outlets<br />

Typ 22: Streams <strong>in</strong> the marshes<br />

Typ 23: Baltic Sea tributaries <strong>in</strong>fluenced by brackish water<br />

2.9 Autecological Information W<strong>in</strong>dow<br />

General<br />

Degradation<br />

Stressor<br />

Organic<br />

Pollution<br />

The data sheet shows all the <strong>in</strong>formation on ecological classification assigned to<br />

each imported species or taxon. This <strong>in</strong>formation is retrieved from the taxa<br />

database.<br />

It is possible to save this data sheet as an EXCEL sheet by press<strong>in</strong>g the “Export to<br />

Excel” button <strong>in</strong> the top frame.<br />

Acidification<br />

21


MANUAL FOR AQEM EUROPEAN STREAM ASSESSMENT PROGRAM VERSION 2.3<br />

FIGURE 13: The Autecological Information W<strong>in</strong>dow.<br />

The first column on the screen shows the taxon name. The follow<strong>in</strong>g columns with<br />

the header “Sample1” to “SampleN” show the abundance of each taxon <strong>in</strong> each<br />

particular sample. The follow<strong>in</strong>g columns display the autecological <strong>in</strong>formation and<br />

ecological classification scores, which have been used to calculate the metrics.<br />

TABLE 3: Explanation of abbreviated column head<strong>in</strong>gs <strong>in</strong> the Autecological <strong>in</strong>formation<br />

W<strong>in</strong>dow.<br />

shortcode Explanation<br />

s<strong>in</strong> German Saprobic Index (new <strong>version</strong>) saprobic score<br />

sgn German Saprobic Index (new <strong>version</strong>) weight<strong>in</strong>g factor<br />

sio German Saprobic Index (old <strong>version</strong>) saprobic score<br />

sgo German Saprobic Index (old <strong>version</strong>) weight<strong>in</strong>g factor<br />

zeu Preference for crenal (spr<strong>in</strong>g) (x out of 10 po<strong>in</strong>ts)<br />

zhy Preference for hypocrenal (spr<strong>in</strong>g-brook) (x out of 10 po<strong>in</strong>ts)<br />

zer Preference for epirhithral (upper-trout region) (x out of 10 po<strong>in</strong>ts)<br />

zmr Preference for metarhithral (lower-trout region) (x out of 10 po<strong>in</strong>ts)<br />

zhr Preference for hyporhithral (greyl<strong>in</strong>g region) (x out of 10 po<strong>in</strong>ts)<br />

zep Preference for epipotamal (barbel region) (x out of 10 po<strong>in</strong>ts)<br />

zmp Preference for metapotamal (brass region) (x out of 10 po<strong>in</strong>ts)<br />

zhp Preference for hypopotamal (brackish water) (x out of 10 po<strong>in</strong>ts)<br />

zli Preference for Littoral (x out of 10 po<strong>in</strong>ts)<br />

zpr Preference for Profundal (x out of 10 po<strong>in</strong>ts)<br />

22


MANUAL FOR AQEM EUROPEAN STREAM ASSESSMENT PROGRAM VERSION 2.3<br />

shortcode Explanation<br />

hpe Preference for microhabitat Pelal (x out of 10 po<strong>in</strong>ts)<br />

har Preference for microhabitat Argyllal (x out of 10 po<strong>in</strong>ts)<br />

hps Preference for microhabitat Psammal (x out of 10 po<strong>in</strong>ts)<br />

hak Preference for microhabitat Akal (x out of 10 po<strong>in</strong>ts)<br />

hli Preference for microhabitat Lithal (x out of 10 po<strong>in</strong>ts)<br />

hph Preference for microhabitat Phytal (x out of 10 po<strong>in</strong>ts)<br />

hpo Preference for microhabitat POM (x out of 10 po<strong>in</strong>ts)<br />

hot Preference for other microhabitats (x out of 10 po<strong>in</strong>ts)<br />

Current preference; 0 = no <strong>in</strong>formation available; 1 = limnobiont; 2 = limnophil;<br />

cup 3 = limno- to rheophil; 4 = rheo- to limnophil; 5 = rheophil; 6 = rheobiont;<br />

7 = <strong>in</strong>different<br />

fgr Feed<strong>in</strong>g type grazer and scrapers (x out of 10 po<strong>in</strong>ts)<br />

fmi Feed<strong>in</strong>g type m<strong>in</strong>ers (x out of 10 po<strong>in</strong>ts)<br />

fxy Feed<strong>in</strong>g type xylophagous taxa (x out of 10 po<strong>in</strong>ts)<br />

fsh Feed<strong>in</strong>g type shredders (x out of 10 po<strong>in</strong>ts)<br />

fga Feed<strong>in</strong>g type gatherers/collectors (x out of 10 po<strong>in</strong>ts)<br />

faf Feed<strong>in</strong>g type active filter feeders (x out of 10 po<strong>in</strong>ts)<br />

fpf Feed<strong>in</strong>g type passive filter feeders (x out of 10 po<strong>in</strong>ts)<br />

fpr Feed<strong>in</strong>g type predators (x out of 10 po<strong>in</strong>ts)<br />

fpa Feed<strong>in</strong>g type parasites (x out of 10 po<strong>in</strong>ts)<br />

fot Other feed<strong>in</strong>g types (x out of 10 po<strong>in</strong>ts)<br />

acidclass Acid Class accord<strong>in</strong>g to Braukmann & Biss<br />

lss Locomotion type: swimm<strong>in</strong>g/scat<strong>in</strong>g (x out of 10 po<strong>in</strong>ts)<br />

lsd Locomotion type: swimm<strong>in</strong>g/div<strong>in</strong>g (x out of 10 po<strong>in</strong>ts)<br />

lbb Locomotion type: burrow<strong>in</strong>g/bor<strong>in</strong>g (x out of 10 po<strong>in</strong>ts)<br />

lsw Locomotion type: sprawl<strong>in</strong>g/wak<strong>in</strong>g (x out of 10 po<strong>in</strong>ts)<br />

lse Locomotion type: (semi)sessil (x out of 10 po<strong>in</strong>ts)<br />

lot Locomotion type: other (x out of 10 po<strong>in</strong>ts)<br />

szx Saprobic valence Zel<strong>in</strong>ka&Marvan: xenosaprob (x out of 10 po<strong>in</strong>ts)<br />

szo Saprobic valence Zel<strong>in</strong>ka&Marvan: oligosaprob (x out of 10 po<strong>in</strong>ts)<br />

szb Saprobic valence Zel<strong>in</strong>ka&Marvan: beta-mesosaprob (x out of 10 po<strong>in</strong>ts)<br />

sza Saprobic valence Zel<strong>in</strong>ka&Marvan: alpha-mesosaprob (x out of 10 po<strong>in</strong>ts)<br />

szp Saprobic valence Zel<strong>in</strong>ka&Marvan: polysaprob (x out of 10 po<strong>in</strong>ts)<br />

szs Saprobic <strong>in</strong>dex Zel<strong>in</strong>ka&Marvan: sabrobic score<br />

szg saprobic <strong>in</strong>dex Zel<strong>in</strong>ka&Marvan weight<strong>in</strong>g factor<br />

masg MAS Group (MAS = Mayfly Average Score)<br />

mass MAS Score<br />

masl MAS Score (large river)<br />

masgl MAS Group (large river)<br />

NSX Netherland Saprobic valence xenosaprob (x out of 10 po<strong>in</strong>ts)<br />

NSO Netherland Saprobic valence oligosaprob (x out of 10 po<strong>in</strong>ts)<br />

NSB Netherland Saprobic valence beta-mesosaprob (x out of 10 po<strong>in</strong>ts)<br />

NSA Netherland Saprobic valence alpha-mesosaprob (x out of 10 po<strong>in</strong>ts)<br />

NSP Netherland Saprobic valence polysaprob (x out of 10 po<strong>in</strong>ts)<br />

German Fauna Index <strong>in</strong>dicator value D01(Small sand bottom streams <strong>in</strong> the<br />

IVD01 German lowlands)<br />

German Fauna Index <strong>in</strong>dicator value D02 (Organic type brook <strong>in</strong> the German<br />

IVD02 lowlands)<br />

23


MANUAL FOR AQEM EUROPEAN STREAM ASSESSMENT PROGRAM VERSION 2.3<br />

shortcode Explanation<br />

German Fauna Index <strong>in</strong>dicator value D03 (Mid-sized sand bottom streams <strong>in</strong> the<br />

IVD03 German lowlands)<br />

German Fauna Index <strong>in</strong>dicator value D04 (Small streams <strong>in</strong> lower mounta<strong>in</strong>ous<br />

IVD04 areas of Central Europe)<br />

German Fauna Index <strong>in</strong>dicator value D05 (Mid-sized streams <strong>in</strong> lower mounta<strong>in</strong>ous<br />

IVD05 areas of Central Europe)<br />

PTI Potamon Typie Index (Schöll & Haybach)<br />

Mod1 Austrian Sensitive Taxa score<br />

czx Czech Saprobic Index valence xenosaprob<br />

czo Czech Saprobic Index valence oligosaprob<br />

czb Czech Saprobic Index valence beta-mesosaprob<br />

cza Czech Saprobic Index valence alpha-mesosaprob<br />

czp Czech Saprobic Index valence polysaprob<br />

czsi Czech Saprobic Index valence saprobic score<br />

czv Czech Saprobic Index weight<strong>in</strong>g factor<br />

AcidScore Acid Score Hendrikson & Med<strong>in</strong><br />

ECO_P ECO_P score<br />

RTI ECO score<br />

RIB Rheo<strong>in</strong>dex accord<strong>in</strong>g to Bann<strong>in</strong>g<br />

AHT1 Stone dwell<strong>in</strong>g taxa score<br />

rst<br />

acidclass<br />

reproduction strategy (1= r-strategy or 2=K-strategy)<br />

new Acid class<br />

dsfis DSFI Family (DSFI = Danish Stream Fauna Index)<br />

dsfi1 DSFI Indicator group 1<br />

dsfi2 DSFI Indicator group 2<br />

dsfi3 DSFI Indicator group 3<br />

dsfi4 DSFI Indicator group 4<br />

dsfi5 DSFI Indicator group 5<br />

dsfi6 DSFI Indicator group 6<br />

ibef IBE Family (IBE = Indice Biotico Esteso)<br />

ibeg IBE Indicator Group<br />

ibell IBE Limit (low)<br />

ibelh IBE Limit (high)<br />

bbif BBI Family (BBI = Belgian Biotic Index)<br />

bbig BBI Indicator group<br />

ID_FAM ID of the family<br />

Subfamily Subfamily name<br />

ID_GC ID of the order<br />

Family Family name<br />

TaxaGroup Name of the taxonomic group<br />

Port1 Score of the Portuguese Index<br />

bmwp BMWP Score<br />

bmwpf BMWP Family<br />

bmwpe BMWP Score Spa<strong>in</strong><br />

bmwpef BMWP Family Spa<strong>in</strong><br />

24

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