A Pictorial Guide for the Identification of Mold ... - OAR@ICRISAT
A Pictorial Guide for the Identification of Mold ... - OAR@ICRISAT
A Pictorial Guide for the Identification of Mold ... - OAR@ICRISAT
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Detection Technique<br />
Eight hundred grains from each sample were examined to identify fungi up to <strong>the</strong> species<br />
level. Each grain sample was subjected to four treatments (200 grains treatment 1 ):<br />
1. Grains were surface sterilized in 1 % sodium hypochlorite (NaOCI) [prepared from Clorox®<br />
(Clorox Company, Oakland, CA 94612, USA) containing 5.25% NaOCI] without fungicide<br />
treatment.<br />
2. Grains were sterilized in NaOCI, and treated with benomyl (0.05%) [Benefit ® 50 WP<br />
(benomyl 50% WP), EID Parry (India)].<br />
3. Grains were sterilized in NaOCI and treated with benomyl.<br />
4. Grains were not sterilized and no benomyl treatment.<br />
The grains were transferred to pre-sterilized petri dish humid chambers @ 25 grains dish 1<br />
(Fig. 1 a, b) under aseptic conditions, and were incubated <strong>for</strong> 5 days at 28±1 °C in an incubator<br />
(Percival®) with a 12-h light cycle <strong>for</strong> observation. The fungi mentioned in this bulletin were<br />
encountered across <strong>the</strong> treatments, storage conditions, seasons, and cultivars. The effects <strong>of</strong><br />
all <strong>the</strong>se factors on mean frequency <strong>of</strong> occurrence <strong>of</strong> individual fungi are published separately.<br />
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