Michele Rhodes - Fox Chase Cancer Center

Experience
Michele Rhodes
7/2004 to date Scientific Assistant, Blood Cell Development and Cancer Program,
Fox Chase Cancer Center, Philadelphia, PA 19111
1/96 to 6/2004 Scientific Technician II, Immunobiology Working Group, Basic
Sciences, Fox Chase Cancer Center, Philadelphia, PA 19111
Cloning and subcloning into GFP-expression, retroviral vectors.
Transfection and infection into mammalian cell lines. Analysis of
expression by flow cytometry, Western blots, metabolic labeling and
biotin cell-surface labeling. Cloning into procaryotic GST-expression
systems to generate proteins for antibody production.
6/93 to 12/95 Scientific Technician II, Population Science,
Fox Chase Cancer Center, Philadelphia, PA 19111
Preliminary screening of populations of cancer patients and control
groups for polymorphisms in the gene sequence for detoxifying enzymes,
dt diapharase and epoxide hydroxylase. Included radiolabeled PCR
reactions with products ran on non denaturing polyacrylamide gels,
(SSCP) gels. Scoring and analysis of results and input of data into
computerized database.
7/90 to 1/93 Research Assistant III, Heart Development Group, Department of
Cell Biology & Anatomy, Medical College of Georgia, Augusta, Ga
30912
Managed and maintained a molecular biology lab, and under limited
supervision coordinated and supervised activities of students, postdoctoral
fellows and a lab helper. Gathered and interpreted data.
Participated in project planning, the development of new research
techniques and protocols, and in finding and adapting already existing
research protocols concerning molecular biology, particularly cDNA
libraries.
7/88 to 6/90 Research Assistant II, Heart Development Group, Department of Cell
Biology & Anatomy, Medical College of Georgia, Augusta, Ga 30912
12/87 to 6/88 Research Assistant I, Heart Development Group, Department of Cell
Biology & Anatomy, Medical College of Georgia, Augusta, Ga 30912
7/86 to 11/86 Research Assistant I, Department of Pharmacology & Toxicology,
Medical College of Georgia, Augusta, Ga 30912
Studies of substance P levels in the dorsal horn of the spinal cord of
laboratory animals and changes resulting from pain stimulus. Included
chronic and acute exposures to acrylamide. Animal surgery, sample
fixation and histoprep. Frozen sectioning and antibody staining. Analysis
with densitometry.
9/85 to 6/86 Laboratory Technician II, Department of Pharmacology &
Toxicology, Medical College of Georgia, Augusta, Ga 30912

Education
1981-1985 B.S. Physics, Augusta State University, Augusta, Ga 30904
Skills
Cell culture, flow cytometry, metabolic labeling, cell-surface biotinylation, viral
transfection and infection, cell stimulation and down modulation assays, procaryotic and
eucaryotic expression systems, immunoprecipitaions and recapture assays, Western
blots, cDNA libraries, subtractive hybridizations, Northerns, Southerns, Cloning,
sequencing, PCR, RT-PCR, SSCP gels, PAGE gels, immunohistochemistry, histology.
Publications
Manuscripts
Lauritsen JP, Kurella S, Lee SY, Lefebvre JM, Rhodes M, Alberola-Ila J, Wiest DL.
(2008) Egr2 is required for Bcl-2 induction during positive selection. J Immunol.
181(11):7778-85.
Haks MC, Lefebvre JM, Lauritsen JP, Carleton M, Rhodes M, Miyazaki T, Kappes DJ,
Wiest DL. (2005). Attenuation of gammadeltaTCR signaling efficiently diverts
thymocytes to the alphabeta lineage. Immunity 22(5):595-606.
Lefebvre JM, Haks MC, Carleton MO, Rhodes M, Sinnathamby G, Simon MC, Eisenlohr
LC, Garrett-Sinha LA, Wiest DL. (2005) Enforced expression of Spi-B reverses T
lineage commitment and blocks beta-selection. J Immunol. 174(10):6184-94.
La Gruta NL, Liu H, Dilioglou S, Rhodes M, Wiest DL, Vignali DA. (2004).
Architectural changes in the TCR:CD3 complex induced by MHC:peptide ligation. J
Immunol. 172(6):3662-9.
Haks MC, Belkowski SM, Ciofani M, Rhodes M, Lefebvre JM, Trop S, Hugo P, Zúñiga-
Pflücker JC, Wiest DL. (2003). Low activation threshold as a mechanism for ligandindependent
signaling in pre-T cells. J Immunol. 170(6):2853-61.
Liu H, Rhodes M, Wiest DL, Vignali DA. (2000). On the dynamics of TCR:CD3
complex cell surface expression and downmodulation. Immunity 13(5):665-75.
Trop S, Rhodes M, Wiest DL, Hugo P, Zúñiga-Pflücker JC. 2000). Competitive
displacement of pT alpha by TCR-alpha during TCR assembly prevents surface
coexpression of pre-TCR and alpha beta TCR. J Immunol. 165(10):5566-72.
Berger MA, Carleton M, Rhodes M, Sauder JM, Trop S, Dunbrack RL, Hugo P, Wiest
DL. (2000). Identification of a novel pre-TCR isoform in which the accessibility of the
TCR beta subunit is determined by occupancy of the 'missing' V domain of pre-T alpha.
Int Immunol. 12(11):1579-91.
Carleton M, Ruetsch NR, Berger MA, Rhodes M, Kaptik S, Wiest DL. (1999). Signals
transduced by CD3epsilon, but not by surface pre-TCR complexes, are able to induce
maturation of an early thymic lymphoma in vitro. J Immunol. 163(5):2576-85.

Berger MA, Davé V, Rhodes MR, Bosma GC, Bosma MJ, Kappes DJ, Wiest DL. (1997).
Subunit composition of pre-T cell receptor complexes expressed by primary thymocytes:
CD3 delta is physically associated but not functionally required. J Exp Med. 186(9):1461-
7.
Hutson, M.R. Rhodes, M.R. Kirby, M.L. (1997). Differential expression of a proteasomal
subunit during chick development. Biochem. Biophys. Res. Comm. , 234(1):216-23.