CURRICULUM VITAE NAME: Siddharth Balachandran ADDRESS ...

Date: July 15, 2013
Name: Siddharth Balachandran
Address: Fox Chase Cancer Center
333 Cottman Avenue
Philadelphia, PA 19111
Phone: 215-214-1527
Email: Sid.Balachandran@fccc.edu
Citizenship: India (US Permanent Resident)
Education:
CURRICULUM VITAE
B.S., Chemistry, Angelo State University, San Angelo, TX 1995
Ph.D., Immunology & Molecular Pathogenesis, Emory University, Atlanta, GA 2001
Postgraduate Training:
Postdoctoral Research Associate, Department of Microbiology & Immunology,
University of Miami Miller School of Medicine, Miami, FL 2001-2005
Faculty Appointments:
Research Assistant Professor, Department of Microbiology & Immunology, 2006-2007
University of Miami Miller School of Medicine, Miami, FL
Associate Member, Institute for Cancer Research, Fox Chase Cancer Center, 2007-2009
Philadelphia, PA
Assistant Professor, Institute for Cancer Research, Fox Chase Cancer Center, 2009-present
Philadelphia, PA
Adjunct Assistant Professor, Department of Biochemistry, Temple University, 2013-present
Philadelphia, PA
Awards and Honors:
Young Investigator of the Year, International Society for Interferon and Cytokine Research 2000
Best Research Presentation, Eastern Student Research Forum, University of Miami 2000
First Prize, Zubrod Annual Poster Competition, University of Miami 2005
Membership in Professional and Scientific Societies:
International Society for Interferon and Cytokine Research
American Association for Cancer Research
American Society for Microbiology

National and International Committee Service:
Member, Immunology and Virology Study Section, American Heart Association 2008-present
Editorial Positions:
Ad hoc reviewer: Cancer Research, Journal of Biological Chemistry, Journal of
Immunology, Journal of Virology, Molecular and Cellular Biology,
Oncogene, PLoS Pathogens, PLoS One 2010-present
Editorial Board member: Molecular and Cellular Biology 2013-2015
Academic Committees, Fox Chase Cancer Center:
Postdoctoral Fellowship Review Committee Member (Glenn Rall, Chair) 2009-present
Co-organizer, F.M. Kirby Inflammation Signaling Symposium 2011-present
Biostatistics and Bioinformatics Steering Committee (Margie Clapper, Chair) 2012-present
Radiation Safety Advisory Committee (Greg Adams, Chair) 2012-present
Invited Presentations in Conferences:
Keystone Symposium “NF-kappa B in Inflammation & Disease”, Santa Fe, NM. 1/2010
Henry & Elsa Jiler American Cancer Society Professors Meeting,
Atlanta, GA. 1/2011
Keystone Symposium “Cell Death Pathways: Beyond Caspases”, Banff, Canada. 3/2012
American Association of Immunology Annual Meeting, Boston, MA. 5/2012
Invited Seminars:
Bice Perussia Immunobiology Symposium, Thomas Jefferson University, Philadelphia, PA. 5/2008
Department of Biochemistry, Temple University, Philadelphia, PA. 1/2009
Drexel Institute for Biotechnology and Virology Research, Doylestown, PA. 2/2011
F.M. Kirby Inflammation Signaling Symposium, Fox Chase Cancer Center, Philadelphia, PA. 10/2011
Department of Immunology, H. Lee Moffitt Cancer Center, Tampa, FL. 3/2012
Temple Autoimmunity Center, Temple University, Philadelphia, PA. 2/2013
Department of Viral Oncology, Johns Hopkins University, Baltimore, MD 4/2013
Teaching:
University of Miami, Miami, FL
Virology/Immunology Course (Graduate level, 3 credits) 2004-2007
Thomas Jefferson University, Philadelphia, PA
Immunology Course (Graduate level, 3 credits) 2009-present
Arcadia University, Glenside, PA, Immunology Course (Undergraduate level, 3 credits) 2010
Fox Chase Cancer Center, Philadelphia, PA
Scientific Writing Course (Postdoctoral level) 2011-present

Mentorship History (Postdoctoral Fellows, Graduate Students, Other)
Current Postdoctoral Trainees:
Roshan Thapa, M.D., Postdoctoral Associate 2011-present
M.D., Manipal College of Medical Sciences, Nepal
Shoko Nogusa, Ph.D., Postdoctoral Associate 2010-present
Ph.D., Immunology, Drexel University, Philadelphia, PA
Past Postdoctoral Trainees:
Suresh Basagoudanavar 2007-2010
Ph.D., Animal Biotechnology, Indian Veterinary Research Institute, Izatnagar, India
Current position: Senior Scientist, Indian Center for Agricultural Research, Bangalore, India
Mathilde Poussin 2007-2009
Ph.D., Enzymatic Engineering Bioconversion and Microbiology,
Université de Picardie Jules Verne, Amiens, France
Current position: Lab Manager (Daniel Powell Lab), University of Pennsylvania
Dissertation Committees:
Sarah Cavanaugh, Dept. of Microbiology, Drexel University (Ph.D.) 2010- 2013
Emily Hopewell, Dept. of Immunology, Moffitt Cancer Center, 2012
(Ph.D.; External Examiner)
Jimson D’Souza, Fox Chase Cancer Center (M.S.) 2009-2010
Tatiana Pazina, Fox Chase Cancer Center (M.S.) 2010-2011
Anna Budina, Fox Chase Cancer Center (M.S.) 2010-2011
Maria Shubina, Fox Chase Cancer Center (M.S.) 2011-2012
Galina Gristina, Fox Chase Cancer Center (M.S.)
Mohit Sehgal, Dept. of Microbiology, Drexel University (Ph.D.) 2012-present
Tanu Singh, Dept. of Genetics, Drexel University (Ph.D) 2012-present
Nehal Solanki-Patel, Dept. of Microbiology, Drexel University (Ph.D.) 2013-present
Maria Shubina, Department of Biochemistry, Temple University 2013-present
Bibliography:
Research Publications (peer reviewed):
1. Balachandran, S., Kim, C.N., Yeh, W.-C., Mak, T.W., Bhalla, K., Barber, G.N. Activation of the
dsRNA-dependent protein kinase, PKR, induces apoptosis through FADD-mediated death
signaling. EMBO J. 17:6888-6902, 1998.
2. Balachandran, S., Roberts, P.C., Kipperman, T., Bhalla, K.N., Compans, R.W., Archer, D.R.,
Barber, G.N. Alpha/beta interferons potentiate virus-induced apoptosis through activation of the
FADD/Caspase-8 death signaling pathway. J. Virol. 74:1513-1523, 2000.

3. Balachandran, S., Roberts, P.C., Brown, L.E., Truong, H., Pattnaik, A.K., Archer, D.R., Barber,
G.N. Essential role for the dsRNA-dependent protein kinase PKR in innate immunity to viral
infection. Immunity 13:129-141, 2000.
4. Heylbroeck, C., Balachandran, S., Servant, M., DeLuca, C., Barber, G.N., Lin, R., Hiscott, J. The
IRF-3 transcription factor mediates Sendai virus-induced apoptosis. J. Virol. 74:3781-3792, 2000.
5. Balachandran, S., Barber, G.N. Vesicular Stomatitis Virus (VSV) therapy of tumors. IUBMB Life
50:135-138, 2000.
6. Ezelle, H.J., Balachandran, S., Sicheri, F., Polyak, S.J., Barber, G.N. Analyzing the mechanisms
of interferon-induced apoptosis using CrmA and hepatitis C virus NS5A. Virology 281:124-137,
2001.
7. Balachandran, S., Porosnicu, M., Barber, G.N. Oncolytic activity of vesicular stomatitis virus is
effective against tumors exhibiting aberrant p53, Ras, or myc function and involves the induction of
apoptosis. J. Virol. 75:3474-3479, 2001.
8. Saunders, L.R., Perkins, D.J., Balachandran, S., Michaels, R., Ford, R., Mayeda, A., Barber, G.N.
Characterization of two evolutionarily conserved, alternatively spliced nuclear phosphoproteins,
NFAR-1 and -2, that function in mRNA processing and interact with the double-stranded RNAdependent
protein kinase, PKR. J. Biol. Chem. 276:32300-32312, 2001.
9. Grandvaux, N., Servant, M.J., tenOever, B., Sen, G.C., Balachandran, S., Barber, G.N., Lin, R.,
Hiscott, J. Transcriptional profiling of interferon regulatory factor 3 target genes: direct
involvement in the regulation of interferon-stimulated genes. J. Virol. 76:5532-5539, 2002.
10. Pataer, A., Vorburger, S.A., Barber, G.N., Chada, S., Mhashilkar, A.M., Zou-Yang, H., Stewart,
A.L., Balachandran, S., Roth, J.A., Hunt, K.K., Swisher, S.G. Adenoviral transfer of the
melanoma differentiation-associated gene 7 (mda7) induces apoptosis of lung cancer cells via upregulation
of the double-stranded RNA-dependent protein kinase (PKR). Cancer Res. 62:2239-
2243, 2002.
11. Balachandran, S., Barber, G.N. Defective translational control facilitates vesicular stomatitis virus
oncolysis. Cancer Cell 5:51-65 2004.
12. Balachandran, S., Thomas, E., Barber, G.N. A FADD-dependent innate immune mechanism in
mammalian cells. Nature 432:401-405, 2004.
13. Pataer, A., Vorburger, S.A., Chada, S., Balachandran, S., Barber, G.N., Roth, J.A., Hunt, K.K.,
Swisher, S.G. Melanoma differentiation-associated gene-7 protein physically associates with the
double-stranded RNA-activated protein kinase PKR. Mol. Ther. 11:717-723, 2005.
14. Balachandran, S., Venkataraman, T., Fisher, P.B., Barber, G.N. Fas-associated death domaincontaining
protein-mediated antiviral innate immune signaling involves the regulation of Irf7. J.
Immunol. 178:2429-2439, 2007.

15. Goodman, A.G., Smith, J.A., Balachandran, S., Perwitasari, O., Proll, S.C., Thomas, M.J., Korth,
M.J., Barber, G.N., Schiff, L.A., Katze, M.G. The cellular protein P58IPK regulates influenza virus
mRNA translation and replication through a PKR-mediated mechanism. J. Virol. 81:2221-2230,
2007.
16. Radtke, A.L., Delbridge, L.M., Balachandran, S., Barber, G.N., O’Riordan, M.X. TBK1 protects
vacuolar integrity during intracellular bacterial infection. PLoS Pathogens 3:e29, 2007.
17. Balachandran, S., Barber, G.N. PKR in innate immunity, cancer, and viral oncolysis. Methods
Mol. Biol. 383:277-301, 2007.
18. Wang, J., Basagoudanavar, S.H., Wang, X., Hopewell, E., Albrecht, R., García-Sastre, A.,
Balachandran, S., Beg, A.A. NF-κB RelA subunit is crucial for early IFN-β expression and
resistance to RNA virus replication. J. Immunol. 185(3):1720-1729, 2010.
19. Basagoudanavar, S.H., Thapa, R.J., Nogusa, S., Wang, J., Beg, A.A., Balachandran, S. Distinct
roles for the NF-κB RelA subunit during antiviral innate immune responses. J. Virol. 85(6):2599-
2610, 2011.
20. Thapa, R.J., Basagoudanavar, S., Nogusa, S., Irrinki, K., Mallilankaraman, K., Beg, A.A., Madesh,
M., Balachandran, S. NF-κB protects cells from gamma interferon-induced RIP1-dependent
necroptosis. Mol. Cell. Biol. 31(14):2934-2946, 2011.
21. Han, Z., Nogusa, S., Nicolas, E., Balachandran, S., Taylor, J.M. Interferon impedes an early step
of Hepatitis Delta Virus infection. PLoS One 6(7):e22415, 2011.
22. Irrinki, K.M., Mallilankaraman, K., Thapa, R.J., Smith, F.J., Jog, N.R., Daswani, V.P., Gandhirajan,
R.K., Kelsen, S.G., Houser, S.R., May, M.J., Balachandran, S., Madesh, M. Requirement of
FADD, NEMO and BAX/BAK for aberrant mitochondrial function in TNFα-induced necrosis.
Mol. Cell. Biol. 31(18):3745-3758, 2011.
23. Balachandran, S., Beg, A.A. Defining emerging roles for NF-κB in antivirus responses: revisiting
the interferon-β enhanceosome paradigm. PLoS Pathog. 7(10):e1002165, 2011.
24. O’Donnell, L.A., Conway, S., Rose, R.W., Nicolas, E. Slifker, M.J., Balachandran, S., Rall, G.F.
STAT1 independent control of a neurotropic measles virus infection in primary neurons and
challenged mice. J. Immunol. 188(4):1915-1923, 2012.
25. Rao, S., Lee, S.-Y., Gutierrez, A., Perrigoue, J., Thapa, R.J., Tu, Z., Jeffers, J.R., Rhodes, M.,
Anderson, S., Oravecz, T., Hunger, S.P., Timakhov, R.A., Zhang, R., Balachandran, S., Zambetti,
G.P., Testa, J.R., Look, A.T., Wiest, D.L. Inactivation of ribosomal protein L22 promotes
transformation by induction of the stemness factor, Lin28B. Blood 120(18):3764-3773, 2012.
26. Steen, H.C., Nogusa, S., Thapa, R.J., Basagoudanavar, S.H., Gill, A., Merali, S., Barrero, C.A.,
Balachandran, S., Gamero, A.M. Identification of a potential regulatory role for STAT2 Serine
287 in type I interferon-induced cellular responses. J. Biol. Chem. 288(1):747-58, 2013.
27. Balachandran, S., Adams, G.P. Interferon-γ-induced necrosis: an anti-tumor biotherapeutic
perspective. J. Interferon Cytokine Res. 33(4):171-80, 2013.

28. Chen, P., Nogusa, S., Thapa, R.J., Simmons, H., Shaller, C., Peri, S., Adams, G.R., Balachandran,
S. Anti-CD70 immunocytokines for exploitation of interferon-γ-induced programmed necrosis in
renal cell carcinoma. PLoS One 8(4): e61446, 2013.
29. Goulet, M.-L., Olagnier, D., Xu, Z., Paz, S., Belgnaoui, S.M., Lafferty, E., Janelle, V., Arguello,
M., Paquet, M., Ghneim, K., Richards, S., Smith, A., Wilkinson, P., Cameron, M., Kalinke, U.,
Qureshi, S., Lamarre, A., Haddad, E.K., Sekaly, R.P., Peri, S., Balachandran, S., Lin, R., Hiscott,
J. Systems analysis of a RIG-I agonist inducing broad spectrum inhibition of virus infectivity.
PLoS Pathog. 9(4): e1003298, 2013.
30. Garipov, A., Li, H., Thapa, R., Balachandran, S., Zhang, R. NF-Y underlies EZH2 upregulation
and is essential for proliferation of human epithelial ovarian cancer cells. Mol. Cancer Res.
11(4):360-9, 2013.
31. Rubio, D., Xu, R.-H., Remakus, S., Krouse, T.E., Truckenmiller, M.E., Thapa, R.J., Balachandran,
S., Alcamí, A., Norbury, C.C., Sigal, L.J. Cross-talk between the type I interferon and NF-κB
pathways rescues resistance to a viral disease. Cell Host Microbe 13(6): 701-710, 2013.
32. Chen, P., Balachandran, S. Development of interferon-γ-based immunocytokines targeting renal
cancer. OncoImmunology 2(7): e24964, 2013.
33. Thapa, R.J., Chen, P., Cheung, M., Nogusa, S., Pei, J., Peri, S., Testa, J.R., Balachandran, S. NFκB
inhibition by bortezomib permits interferon-γ-activated RIP1 kinase-dependent necrosis in renal
cell carcinoma. Mol. Cancer Ther. (In press).
34. Thapa, R.J., Nogusa, S., Chen, P., Maki, J., Lerro, A.T., Rall, G.F., Degterev, A., Balachandran, S.
Interferon-induced RIP1/RIP3-mediated necrosis requires PKR and is licensed by FADD and
caspases. Proc. Natl. Acad. Sci. USA (In press).
Editorials, Reviews and Chapters:
Balachandran, S., Barber, G.N. PKR in innate immunity, cancer, and viral oncolysis. Methods Mol.
Biol. 383:277-301, 2007.
Balachandran, S., Beg, A.A. Defining roles for NF-κB in antivirus responses: revisiting the interferonβ
enhanceosome paradigm. PLoS Pathog. 7(10):e1002165, 2011.
Balachandran, S., Adams, G.P. Interferon-γ-induced necrosis: an anti-tumor biotherapeutic
perspective. J. Interferon Cytokine Res. 33(4):171-180, 2013.
Chen, P., Balachandran, S. Development of interferon-γ-based immunocytokines targeting renal
cancer. OncoImmunology 2(7):e24964, 2013.