ECL Primeの基本プロトコール

NAME
ECL Prime
71-3477-81

MEMO
ECL Prime
1
2
or
3
PBS-T or TBS-T
4
PBS-T or TBS-T
ECL Prime
5
CCD
ImageQuant LAS 4000 CCD
X
X
X
1

ECL Prime 1/5
ECL Prime
MEMO
1
ECL Prime Western Blotting Detection ReagentsRPN2232
HRP ECL
Prime Starter Kit Core DualVue 72-AS01-21
10 8cm 2 2
1.
10
PVDF 100%
SDS-PAGE
Rainbow Molecular Weight Markers ECL DualVue Western Blotting Markers
SDS-PAGE Hybond-P Hybond-ECL
2 8 3
min mA
min mA
2

ECL Prime 2/5
2.
MEMO
5.
2 5 ECL Prime Blocking ReagentRPN418in PBS-T or TBS-T
5 in PBS-T or TBS-T
PBS-T or TBS-T
CCD or
1
PBS-T or TBS-T Tween 20
2 1
4
3 2 2
Check 2 2
1cm 2 4ml8 10 cm 320 ml
3
1 PBS-T or TBS-T
1 mg/ml 11000 150000
2 1
3
2 215 15 3
Check 2 2 15 5 5 5
1cm 2 4ml8 10 cm 320 ml
3

ECL Prime 3/5
4.
MEMO
1 PBS-T or TBS-T HRP
ECL DualVue Western Blotting Markers HRP S-protein
1 mg/ml 150000 1250000
-HRP
1 PBS-T or TBS-T
2 1
3
2 215 15 3
Check 2 2 15 5 5 5
1cm 2 4ml8 10 cm 320 ml
1
4 PBS-T or TBS-T HRP RPN1231-2ML
5 45
45 60
6
2 215 15 3
Check 2 2 15 5 5 5
1cm 2 4ml8 10 cm 320 ml
4

ECL Prime 4/5
5.
ImageQuant LAS 4000 CCD X
CCD
MEMO
1ECL Prime Solution ASolution B
2Solution ASolution B 11
1cm 2 0.1 ml 8 10 cm 4 ml Solution A4 ml
Solution B
2
CCD CCD
ImageQuant LAS 4000 control software CCD
1
2 5
3
4 OHP EPI
CCD
5 EPI ImageQuant LAS 4000
6 Chemiluminescence
60
8 10 cm 2
X High
Increment
7
PBS-T TBS-T 10 2
5. 1
5

ECL Prime 5/5
MEMO
X
5 X
X
X
6 30
5
7 X
PBS-T or TBS-T 2 8 1
PBS-T TBS-T 10 2
5. 1
6

TEL03-5331-9336
FAX03-5331-9370
e-mailTech-JP@ge.com
Web
www.gelifesciences.co.jp>> ECL
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