roote-prokop-2013-g3-drosophila-genetics-training-all

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Balancers and stock keeping only heterozygous flies survive and maintain the stock • balancers carry easily identifiable dominant and recessive markers • balancers are homozygous lethal or sterile • the products of recombination involving balancers are lethal • During mating schemes, balancers can be used to prevent unwanted recombination - providing an additional means to the recombination rule. • You can use balancers and their dominant markers strategic<strong>all</strong>y to follow markerless chromosomes through mating schemes.
Rules to be used here: • 'X' indicates the crossing step; female is shown on the left, male on the right • sister chromosomes are separated by a horizontal line, different chromosomes are separated by a semicolon, the 4 th chromosome will be neglected • maternal chromosomes (inherited from mother) are shown above, paternal chromosomes (blue) below separating line • the first chromosome represents the sex chromosome, which is either X or Y - females are X/X, males are X/Y • generations are indicated as P (parental), F1, 2, 3.. (1 st , 2 nd , 3 rd .. filial generation) • to keep it simple: dominant markers start with capital, recessive markers with lower case letters
Page 1 and 2: INVESTIGATION How to Design a Genet
Page 3 and 4: The second section explains why gen
Page 5 and 6: tractable model organisms that can
Page 7 and 8: A. Prokop - A rough guide to Drosop
Page 37 and 38: Assign the number of the correct ge
Page 39 and 40: STEP 1: Remind yourself of the key
Page 41 and 42: STEP2: Remind yourself of the basic
Page 43 and 44: Law of segregation / linkage groups
Page 45 and 46: Complication: recombination in fema
Page 47 and 48: Balancers and stock keeping • let
Page 49: Balancers and stock keeping • bal
Page 53 and 54: Task: To study the potential effect
Page 55 and 56: Task: To study the potential effect
Page 57 and 58: Task: Recombine P(lacZ,w + ) with l
Page 73 and 74: Drosophila genetics training - Cros
Page 75: Drosophila genetics training - Cros

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