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OECD SIDS<br />
4-<strong>METHYLPENTAN</strong>-2-<strong>OL</strong><br />
4. ECOTOXICITY ID: <strong>108</strong><strong>11</strong>2<br />
DATE: 4.07.2006<br />
4.1 ACUTE/PR<strong>OL</strong>ONGED TOXICITY TO FISH<br />
Type : other: static renewal<br />
Species : Oncorhynchus mykiss (Fish, fresh water)<br />
Exposure period : 96 hour(s)<br />
Unit : mg/l<br />
NOEC : = 105<br />
LC50 : = 359 measured/nominal<br />
Limit test : no<br />
Analytical monitoring : yes<br />
Method : other: OECD 203; EEC 92/69/EEC, C.1; TSCA 40 CFR 797.1440<br />
Year : 2003<br />
GLP : yes<br />
Test substance : other TS<br />
Test substance : Methyl isobutyl carbinol; Lot No. AA0155V178 (received from<br />
Union Carbide Corp., Texas City, TX; 99.9%.<br />
Method : A 96-hour static probe study was conducted at nominal<br />
concentrations of 0, 31.3, 62.5, 125, 250, 500 and 1000 mg/L<br />
(10 fish/concentration). All fish died at 500 and 1000<br />
mg/L. No mortality was observed at or below 250 mg/L. Due<br />
to variability in the measured concentrations of the test<br />
substance between days 2 and 4 of the study, the definitive<br />
study was conducted under static renewal conditions at<br />
nominal concentrations of 105, 150, 214, 306, 438, and 625<br />
mg/L.<br />
The definitive test used a total of 10 fish (5 fish per<br />
replicate, in each of two replicates) exposed to each test<br />
concentration, as well as a control (100% dilution water).<br />
Mortality (no response to touching of the caudal peduncle<br />
and no opercular movement) and sublethal effects (e.g.,<br />
erratic swimming) were recorded at 24, 48, 72 and 96 hours,<br />
and any dead fish were removed. Behavioral and gross<br />
pathological effects were recorded. The following<br />
summarizes the test conditions<br />
Parameter<br />
Condition<br />
Test type<br />
Static renewal<br />
Species<br />
Oncorhynchus mykiss<br />
Supplier<br />
Thomas Fish Co., Anderson, CA<br />
Test duration<br />
96 hours<br />
Temperature 15 ± 2°C<br />
Light cycle<br />
16-hour light/8-hour dark<br />
Feeding prior to test<br />
Standard lab diet<br />
(Aquatic Diet Number 1)<br />
Feeding regime<br />
None (during preceding 48 hour<br />
and during testing)<br />
Test chamber<br />
12 L glass beakers<br />
Water volume<br />
10.0 L<br />
Acclimation / Health<br />
14-day acclimation<br />
< 5%<br />
mortality in 7 days prior to test.<br />
Age/wt of test organisms Juvenile (4.3 ± 0.3<br />
cm; 0.694 ± 0.160 g)<br />
Definitive test concentrations 0, 105, 150, 214, 306,<br />
438, and 625 mg/L<br />
No. replicate /concentration 2<br />
Number of fish per replicate 5<br />
UNEP PUBLICATIONS 43
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