4-METHYLPENTAN-2-OL CAS N°: 108-11-2

OECD SIDS
4-METHYLPENTAN-2-OL
4. ECOTOXICITY ID: 108112
DATE: 4.07.2006
Species
Daphnia magna
Straus
Supplier
Originally from Aquatic Biosystems,
Fort Collins, CO
Test duration
48 hours
Temperature 20 ± 2°C
Light cycle
16-hour light/8-hour dark
Illumination
2050 ± 350 lux (cool-white fluorescent)
Feeding prior to test
Mixture of algae and yeast-ceraphyll trout
Feeding regime
Five times weekly before the test; None
during testing)
Test chamber
250 ml glass beakers
Water volume
200 ml
Age of test organisms Instars; < 24 hours old
Definitive test concentrations 0, 105, 150, 214, 306, 438, and 625 mg/L
No. replicate /concentration 2
Number of daphnia per replicate 10
Dilution water
Adjusted (for hardness) Laboratory
Dilution Water (ALDW)
Original source: Lake Huron
Renewal
Day 0 and ~24 hours
Statistical analyses: An insufficient biological response
(variable, non-linear, and insufficient immobility at the
highest dose level) was observed following 24 hours of
exposure. Therefore the 24-hour EC50 was empirically
determined and conservatively estimated that it would be no
less than the 48-hour EC50 value. The U.S. EPA Trimmed
Spearman-Karber (TSK) Program, Version 1.5 was used to
calculate the 48-hour LC50 values and corresponding percent
trim values. This method was used because of the limited
biological response that did not allow for use of the
preferred probit analyses.
Analytical: Test concentrations were analyzed at 0, 24 and
48 hours using a gas chromatography/flame ionization
detection (GC/FID) method and external standard
quantitation.
Test condition : Species: D. magna
Age: < 24 hours
Test medium: Adjusted (for hardness) Laboratory Dilution
Water
Water quality parameters as measured during the test):
Temperature = 19.9 - 20.9 degrees C
(Days 0, 1, 2);
Dissolved oxygen = 8.2 - 9.9 mg/L
(Days 0, 1, 2);
pH = 7.3 - 7.6 (Days 0, 1, 2);
residual chlorine was