Hot-Plate for Evaluating Thermal Analgesia - Harvard Apparatus

analgesia
Hot-Plate for Evaluating Thermal Analgesia
Hot Plate
Key Features
• Digital set point
• Built-in electronic timer
• Foot switch timing operation
• Computer interface
Parameters Measured
• Time latency to 'paw licking'
• Time latency to 'jumping'
Components Included
• Base with heating plate
• Foot switch
• SeDaCom software
• Cables and connectors
• Certificate of calibration
• Instruction manual
• Set of spare fuses
• 2 year warranty
Options
• LE7000 Thermal printer
Hot-Plate
The LE7406 Hot-Plate performs rapid and precise screening of analgesic
drug properties on small-laboratory animals according to the ‘hot-plate
test’. The animal’s pain sensitivity alterations induced by a specific
experimental context change and/or genetic manipulations can also be
evaluated through this method.
The hot-plate test, initially described by N.B. Eddy and D. Leimbach
(1953), evaluates thermal pain reflexes due to footpad contact with a
heated surface. During the experiments, the animal is confined in a
removable clear acrylic cylinder where the latency time to the first hind
paw or/and jumping responses are measured.
In the LE7406 Hot-Plate, a thick aluminum plate (10 mm) provides a high
temperature stability and even surface distribution. The plate temperature
can be held at a set point between 45 and 62°C (± 0.1ºC) by multiple
proportional feedback circuits that minimize overshoot. A built-in timer
activated by an external foot switch allows precise measurement of
reaction time (0.1 sec precision). A remote foot-switch controls the test
start/stop allowing rapid hands-free experiments. The operator can read
the animal reaction time from the display or from a PC computer using the
SeDaCom software. Trial number, plate temperature and reaction time are
then sent to the PC through a RS-232 port.
Specifications
Base Dimensions
Plate Dimensions
Cylinder Dimensions
Operating Temperature
Reaction Time
Material Composition
Computer Requirements
Maximum Number
of Stations
Power Requirements
Certifications
200 (W) x 300 (D) x 110 (H) mm
200 (D) mm
200 (D) x 250 (H) mm
45 to 62 degrees Celsius; 0.1 steps
3 digits, 0.01 sec increment
Clear methacrylate (animal holder), aluminum (plate)
PC (Windows 95, 98, ME, NT, 2000 and XP)
1 per computer (multiple set-ups also available
under request)
110V or 220V, 50/60Hz
CE compliant
Model Product Order # Price $
LE 7406 Hot-Plate Thermal Analgesia BH1 76-0113 5,070.00
Meter Including SeDaCom Software
Options
LE7000 Thermal Printer BH1 76-0114 1,341.00
Citations
Camarasa J et al. (2006) Association of caffeine to MDMA does not increase antinociception by
potentiates adverse effects of this recreational drug. Brain Res. 1111:72-82. (mice, Spain)
Grillet N et al. (2005) Generation and characterization of Rgs4 mutant mice. Mol. Cell. Biol.
25(10): 4221-4228. (mice, France)
Kim EJ et al. (2005) Safety pharmacology of sibutramine mesylate, an anti-obesity drug. Hum.
Exp. Toxicol. 24(3): 109-119. (Rat, South Korea)
Feria MM et al. (2003) Coexistence of Two Distinct Diurnal Rhythms in Latency of Licking and
Jumping Responses in Mice Hot Plate Tests. Biological Rhythm Res. 29(3):260 – 271 (Mice,
Spain)
Shaheen HM et al. (2000) Effect of Psidium guajava leaves on some aspects of the central
nervous system in mice. Phytotherapy Res. 14(2):107-111 (Mice, United Arab Emirates)
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Harvard Apparatus • phone 508.893.8999 • toll free U.S. 800.272.2775 • fax 508.429.5732 • www.harvardapparatus.com
Panlab | Harvard Apparatus • Spain +34934190709 • international +34834750697 • fax +34934750699 • www.panlab.com

analgesia
Hot/Cold Plate for Testing Animal Sensitivity
Hot/Cold Plate Anagelsia Meter
Key Features
• BSRamp software will allow the user to define
temperature ramps (slope in °C/min, start and
end points) and store results
Parameters Measured
• Animal reaction time to hot or cold stimulus
Components Included
• Stimulation Unit LE7420
• Foot Switch
• USB cable
• BSRamp software
• Instruction manual
• 1 year warranty
Hot/Cold Plate
Panlab/Harvard Apparatus Hot/Cold Plate Analgesia Meter is based on a
metal plate which can be heated to 65°C and cooled to -3°C (with an
ambient temperature between 20°C and 25°C). An electronic thermostat
maintains the plate's temperature and a front panel digital thermometer
displays the current plate temperature.
The animal’s pain sensitivity resulting from exposure to heat or cold is
tested by placing the animal on the surface of the plate and starting a
built-in timer. The operator stops the timer at the instant the animal lifts
its paw from the plate, reacting to the discomfort. The front panel timer
then displays the number of seconds the animal took to react. Animal
reaction time is a measurement of animal resistance to pain and is used
to measure efficacy of analgesics.
The plate is designed to be very simple to use at very fast to reach the set
temperature (as example From ambient to 4°C, the most used threshold
value, it takes less than 10 minutes, and from 4°C to 65°C it takes only 5
minutes). The Hot/Cold Plate, is accurate to less than 0.5°C (EEC metrology
standard) and perfectly constant in the animal holder system. The preset
temperature will not change for more than 0.1°C when a 400g rat is placed
on the plate, and return to the set temperature is almost immediate.
In addition, the instrument can be adjusted to be used for "TEMPERATURE
RAMPS". Predefined by the user, this feature is mainly used for studies
with telemetry implants. In addition to displaying the reaction time, the
Cold/Hot Plate Analgesia Meter is capable of sending the same
information via USB interface to a computer.
The operator can start and stop the timer with the front panel start/stop
switch or with the included footswitch, which allows "hands-free" operation.
Specifications
Temperature Range
Temperature Accuracy
Temperature Uniformity
on Plate
Power Requirements
Dimensions:
Plate
Control Unit
Weight
-3°C to 65°C (in 20°C to 25°C ambient environment,
50% RH)
±0.5°C
±0.5°C
110V/220V automatic, 100W
165 x 165 mm
305 x 280 x 158 mm
6.5 kg
Model Product Order # Price $
LE7420 Hot/Cold Plate Including BH1 76-0112 8,528.00
BSRamp Software
Harvard Apparatus • phone 508.893.8999 • toll free U.S. 800.272.2775 • fax 508.429.5732 • www.harvardapparatus.com
Panlab | Harvard Apparatus • Spain +34934190709 • international +34834750697 • fax +34934750699 • www.panlab.com
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