Christoph Oberprieler The Systematics of Anthemis L. - Herbmedit.org

8
Oberprieler: Anthemis in N Africa
P-DESF
P-LA
RNG
SEV
UPS-Burser
Herb. Bayreuth
Herb. Oberp~ieler
Herb. Vogt
Muséum National d'Histoire Naturelle - Laboratoire de
Phanérogamie Paris (Herbarium R. L. Des fontaines)
Muséum National d'Histoire Naturelle - Laboratoire de
Phanérogamie Paris (Herbarium J. B. P. A. de Monet de
Lamarck)
University of Reading, School of Plant Sciences
Departamento de Biologfa Vegetai y Ecologfa, Universidad de
Sevilla
Botanical Museum at Uppsala University (Herbarium 1. Burser;
microfiche)
Universitiit Bayreuth, Lehrstuhl fiir Ptlanzengeographie
private collection of Ch. Oberprieler (Jena)
private collection of R. Vogt (Berlin)
Specimens were exarnined with a stereo-microscope (Wild M5A), measurements being
performed using the camera-lucida device to slot in a millimetric scale in the corresponding
magnification.
The description of taxa is based on measurements made on dried plants. The variation
ranges cited cover the total observed variation exhibited by a particular taxon. Extreme
values have been placed in parentheses, while mai n range values relate to the interval
covered by the mean ± l standard deviation of a particular character distribution.
A list of specimens seen is presented under each species. Localities are given countrywise
from W to E in the following order: Spain (N African part), Marocco, Algeria, Tunisia,
and Libya. Within each country, administrative districts (Morocco: Province; Algeria:
Wilaya; Tunisia: Gouvernorat; Libya: Baladiyah) are listed in alphabetic order. Within
each district, specimens are listed from N to S. Most of the specimens are given with longitude
and latitude of the collecting locality, written as stated by the collector(s), or taken
from various maps [when they are cited in square brackets]. Distribution maps were drawn
using the public domain map projection program MICROCAM by S. A. Loomer (ftp://ftp.
usma.edulg&ene/gsl/microcarn/).
For comparative carpological studies, achenes were taken from herbarium specimens
and soaked for two days in a mixture (6 : l) of lO % aqueous solution of dioctyl sodium
sulfosuccinate and 96 % ethanol (Peterson & al. 1978). Following dehydration through a
l: 1,3: 7, l : 9 incrementai series of sulfosuccinate/ethanol, with changes every 24 hours,
and a final step in pure 96 % ethanol for 45 min, achenes were subsequently soaked for 4-
6 weeks in a pretreatment solution of Kulzer Technovit 7100® and solidifier no. 1 at 4°C.
After final embedding in a l : 15 mixture of the pretreatment solution and Kulzer solidifier
no. 2, median-transverse and median-Iongitudinal sections of achenes were made using a
rotary microtome. 3-5 flm thick sections from the middle half of the achenes were stained
with toluidine blue and mounted in Vitroclud (Langenbrinck).
For scanning electron microscopy (SEM) achenes were taken from herbarium specimens,
mounted on preparation stubs, and coated with a gold-palladium layer 2 nm thick in
a Polaron sputter-coater. The observations and photography were performed with a Philips
SEM 515 at the Botanical Museum Berlin-Dahlem.