Maximizing Light Utilization Efficiency and Hydrogen Production in ...
Maximizing Light Utilization Efficiency and Hydrogen Production in ...
Maximizing Light Utilization Efficiency and Hydrogen Production in ...
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Cyanobacterial Approach<br />
Replacement of the phycocyan<strong>in</strong>-encod<strong>in</strong>g operon (Go/No-Go Decision)<br />
Wild type Synechocystis genomic DNA<br />
F1<br />
!"# $"#<br />
cpcB cpcA cpcC cpcC cpcD<br />
R5<br />
F3<br />
F1<br />
Kanamyc<strong>in</strong><br />
resistance<br />
!"# $"#<br />
R5<br />
R3<br />
F3<br />
!cpc replacement<br />
R3<br />
WT<br />
!cpc<br />
Expected PCR product size:<br />
WT [bp] !cpc [bp]<br />
F3R3 4683 2126<br />
F1R5 4973 2416<br />
kb<br />
5<br />
4<br />
3<br />
2<br />
F3 F1 F3 F1<br />
R3 R5 R3 R5
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