Maximizing Light Utilization Efficiency and Hydrogen Production in ...

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WT gDNA 5’ cpcB cpcA cpcC cpcC cpcD 3’ bp 1000 850 Δcpc transformant gDNA F4 R3 F4 R4 F4 F5 R5 R3 F5 F4 5’ 3’ Kan F5 F5 R4 R5 positive control R3 R4 R5 Expected product size Primer set Length [bp] F4R3 825 F4R4 894 F4R5 949 F5R3 1008 F5R4 1077 F5R5 1132
Chl Antenna Size vs Light Utilization Efficiency Utilization Efficiency of Absorbed Light Energy • Wild type antenna size = 470 Chl molecules (100%) (PSII=230; PSI=240) Photon use efficiency of WT photosynthesis = ~6-10% Utilization Efficiency of Absorbed Light Energy by WT: ~3-5% • tla1 antenna size = 275 Chl molecules (59% of control) (PSII=115; PSI=160) Photon use efficiency of tla1 photosynthesis = ~20% Utilization Efficiency of Absorbed Light Energy by tla1: ~10% • tla2 antenna size = 195 Chl molecules (42% of control) (PSII=80; PSI=115) Photon use efficiency of tla2 photosynthesis = ~30% Utilization Efficiency of Absorbed Light Energy by tla2: ~15% • Long-term goal: 132 Chl molecules (28% of control) (PSII=37; PSI=95) Photon use efficiency of photosynthesis goal = ~60% Utilization Efficiency of Absorbed Light Energy goal: ~30% UCB-Melis 27
Page 1 and 2: Maximizing Light Utilization Effici
Page 3 and 4: Relevance The TLA concept (TLA = Tr
Page 5 and 6: Relevance Example: Truncated Chl An
Page 7 and 8: Objectives and Approach Objectives:
Page 9 and 10: Microalgae Accomplishments and Prog
Page 11 and 12: PART 2 Cyanobacteria UCB-Melis 11
Page 13 and 14: Cyanobacterial Approach Unit photos
Page 15 and 16: Cyanobacterial Approach Molecular a
Page 17 and 18: Accomplishments and Progress Eviden
Page 19 and 20: Accomplishments and Progress • Su
Page 21 and 22: Proposed Future Work Compete the an
Page 23 and 24: Technical Backup Slides UCB-Melis 2
Page 25: WT gDNA 5’ cpcB cpcA cpcC cpcC cp

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