Maximizing Light Utilization Efficiency and Hydrogen Production in ...

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Cyanobacterial Approach Replacement of the phycocyanin-encoding operon (Go/No-Go Decision) Wild type Synechocystis genomic DNA F1 !"# $"# cpcB cpcA cpcC cpcC cpcD R5 F3 F1 Kanamycin resistance !"# $"# R5 R3 F3 !cpc replacement R3 WT !cpc Expected PCR product size: WT [bp] !cpc [bp] F3R3 4683 2126 F1R5 4973 2416 kb 5 4 3 2 F3 F1 F3 F1 R3 R5 R3 R5
Cyanobacterial Approach Molecular and Physiological Evidence of Transformation (Go/No-Go Decision) Wild type Synechocystis Blue-green phenotype due to PC and Chl pigments Δcpc transformants Green phenotype indicating loss of PC
Page 1 and 2: Maximizing Light Utilization Effici
Page 3 and 4: Relevance The TLA concept (TLA = Tr
Page 5 and 6: Relevance Example: Truncated Chl An
Page 7 and 8: Objectives and Approach Objectives:
Page 9 and 10: Microalgae Accomplishments and Prog
Page 11 and 12: PART 2 Cyanobacteria UCB-Melis 11
Page 13: Cyanobacterial Approach Unit photos
Page 17 and 18: Accomplishments and Progress Eviden
Page 19 and 20: Accomplishments and Progress • Su
Page 21 and 22: Proposed Future Work Compete the an
Page 23 and 24: Technical Backup Slides UCB-Melis 2
Page 25 and 26: WT gDNA 5’ cpcB cpcA cpcC cpcC cp
Page 27 and 28: Chl Antenna Size vs Light Utilizati

Maximizing Light Utilization Efficiency and Hydrogen Production in ...

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