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Maximizing Light Utilization Efficiency and Hydrogen Production in ...

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Accomplishments <strong>and</strong> Progress<br />

Evidence is presented at the genetic <strong>and</strong> molecular levels to<br />

demonstrate successful double homologous recomb<strong>in</strong>ation for<br />

replacement of the cpc DNA operon <strong>in</strong> cyanobacteria.<br />

Further evidence is presented at the biochemical level to show the<br />

ensu<strong>in</strong>g absence of phycocyan<strong>in</strong> light-harvest<strong>in</strong>g pigments from<br />

cyanobacteria (∆cpc stra<strong>in</strong>s), seen by the green coloration of the<br />

latter, as opposed to the blue-green coloration of the wild type <strong>and</strong> by<br />

the absence of phycocyan<strong>in</strong> from the absorbance spectra of the ∆cpc<br />

stra<strong>in</strong>s.<br />

UCB-Melis<br />

17

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