超临界CO2萃取竹叶绿素的实验研究 - ICBR

34 1 

2007 

 

JOURNAL OF BEIJ IN G UN IV ERSIT Y OF CHEMICAL TECHNOLO GY 

Vol. 34 , No. 1 

2007 

 

CO 2 

1 

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(11 

, 100029 ; 21 

, 100102) 

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, 

, 

664 

 

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nm 645 nm , Arnon [7 ] 

70 % , 

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a 

a = 1217 A 664 - 2169 A 645 ; 

 

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b = 2219 A 645 - 4168 A 664 ; 

a + b = 

 

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, 

: 2006207218 

E2mail : 2004000069 @grad. buct. edu. cn 

[125 ] 

 

, 

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© 1994-2007 China Academic Journal Electronic Publishing House. All rights reserved. http://www.cnki.net

1 : CO 2 

93 

 

, CO 2 , 

CO 2 ,4 %12 % , 

 

,, 

10 %, 

 

, 

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, 

 

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1 CO 2 

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Fig. 1 Variation in yield of chlorophyll with temperature 4 , CO 2 , 

212 

, 60 g/ h , 

313 318 323 K 

, CO 2 

, 

2 ,9 

, 60 g/ h 

CO 2 

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, 

CO 2 

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, 27 MPa 

3 

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214 CO 2 

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Fig. 4 Variation in yield of chlorophyll with CO 2 flow rate 

215 

 

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Fig. 2 Variation in yield of chlorophyll with pressure 

213 

 

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, 

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80 min ,CO 2 60 g/ h 

, 

Fig. 5 Variation in yield of chlorophyll with particle 

 

3 size of raw material 

5 


94 

 

2007 

 

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: 323 K 

 

CO 2 27 MPa , ( ) 10 % CO 2 

, 

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80 

 

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min , , 

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CO 2 

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27 MPa 323 K 

 

, 6 : 

[1 ] , , . CO 2 

[4 ] . CO 2 

[J ]. 

6 

,2003 ,13 (2) :7 - 9. 

Fig. 6 Variation in yield of chlorophyll with 

[5 ] HE Chunlian , L I Gucai. Supercritical fluid extraction2 

extraction time 

high performance liquid chromatography determin2ation 

 

, 

, of colchicine in lily [J ]. Natural Product Research and 

, 

60 min Development ,2003 ,15(1) :5 - 8. 

, 

80 min , 

[6 ] , , , . 

, 

80 min 

[J ]. 

, 2005 , 25 (3) : 

106 - 110. 

3 

 

,1996 ,15 (2) :145 - 150. 

CO 2 

 

3153 , 

[J ]. ,1987 ,6 (1) :27 - 31. 

[2 ] DUARTE C , MOLD A O2MARTINS M , GOUV EIA A 

F , et al. Supercritical fluids extraction of red pepper[J ]. 

Journal of Supercritical Fluids , 2004 ,30(2) :155 - 161. 

[3 ] , , , . 

 

K 1 [J ]. 

: 

,2005 ,32 (6) :9 - 11. 

[7 ] . 

[J ]. Extraction of chlorophyll from phyllostachys pubescens 

foliage with supercritical carbon dioxide 

WU Hao 1 J IN J unSu 1 ZHAN G Ze Ting 1 WAN G Ge 2 L U Fang 2 L I Xue 1 

(11 College of Chemical Engineering , Beijing University of Chemical Technology , Beijing 100029 ; 

21 International Centre for Bamboo and Rattan , Beijing 100102 , China) 

Abstract : The extraction of chlorophyll from phyllostachys pubescens foliage has been studied using supercritical 

CO 2 extraction trchnology. The influence of extraction pressure , temperature , time , flow rate of CO 2 , amount 

of entrainer (anhydrous ethanol) , and particle size on the yield of chlorophyll were investigated. The results 

showed that the optimum conditions were a particle size of 60 mesh , temperature 323 K , pressure of 27 MPa , 

CO 2 flow rate of 60 g/ h , time of 80 min , with 10 % entrainer. The yield of chlorophyll under these conditions 

was 31535 % , and the method was highly reproducible. 

Key words : supercritical CO 2 extraction ; phyllostachys pubescens foliage ; chlorophyll

超临界CO2萃取竹叶绿素的实验研究 - ICBR

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