DNA, RNA, and protein purification Catalog ... - Macherey Nagel

DNA, RNA, and
protein purification
Catalog supplement 2013
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Contents
MACHEREY-NAGEL · Bioanalysis catalog supplement 2013
This Bioanalysis catalog supplement 2013 is an addition to the main Bioanalysis catalog 2011 / 2012.
Introducing 11 new products and presenting 4 product updates, the catalog supplement 2013 shows all
latest MACHEREY-NAGEL Bioanalysis developments at a glance.
Contents
Plasmid DNA
Page Category
Transfection-grade plasmid DNA
NucleoBond ® Xtra BAC 2 NEW
Transfection-grade, endotoxin-free plasmid DNA
NucleoBond ® 96 Xtra EF 3 NEW
MACHEREY-NAGEL
Clean-up
PCR clean-up and gel extraction
NucleoSpin ® Gel and PCR Clean-up 4 Update
PCR clean-up
NucleoSpin ® 8 PCR Clean-up 5 Update
NucleoSpin ® 96 PCR Clean-up 5 Update
Genomic DNA clean-up
NucleoSpin ® gDNA Clean-up 7 NEW
NucleoSpin ® gDNA Clean-up XS 8 NEW
RNA
MicroRNA
NucleoSpin ® miRNA Plasma 9 NEW
RNA from blood
NucleoSpin ® RNA Blood 10 NEW
NucleoSpin ® RNA Blood Midi 11 NEW
NucleoSpin ® 8 RNA Blood 12 NEW
NucleoSpin ® 96 RNA Blood 12 NEW
Viral RNA and DNA
Viral RNA and DNA from serum and plasma
NucleoSpin ® Dx Virus 14 NEW
Protein
Purification of His-tag proteins
Protino ® 96 Ni-NTA 15 NEW
Auxiliary tools and transfer media
Sterile filtration
MN Sterilizer CA 16 Update
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MACHEREY-NAGEL
Product information
Apart from the Bioanalysis catalog 2011 / 2012 and the new supplement 2013,
more product information is outlined in diverse marketing materials.
• Product selection guide – summary of the main specifications of all Bioanalysis
products at a glance
• Product guides – overview of certain product groups
• Product flyers – presentation of single products
• Lab focus flyers – selection of products relevant for certain laboratory focuses
For additional information material, please contact MN (tech-bio@mn-net.com)
or see www.mn-net.com/bioflyerscatalog.
Constantly updated product information, application data, literature, and product
news are provided at the MN website: www.mn-net.com/Bioanalysis.
NEWS
MACHEREY-NAGEL introduces the new Bioanalysis icons. Each product chapter
is now represented by a new icon. The icons, in addition to the different colors of
the chapters, allow a fast orientation to easily find the products of interest.
The new icons will be shown in product information, product material, and marketing
material.
Plasmid DNA Clean-up RNA
MN quality
Genomic DNA Viral RNA and DNA Protein
High throughput Accessories Auxiliary tools
EN ISO certification
MACHEREY-NAGEL has been certified under the EN ISO 9001 norm since
1996 and for medical devices under the EN ISO 13485 norm since 2001.
Selected products are CE-certified in accordance with EU Directive 98/79/EC.
Sophisticated German manufacturing and strict quality control lay the foundation
for high quality standards that our products consistently meet. This quality is ensured
by the corresponding MACHEREY-NAGEL seal present on our products.
MACHEREY-NAGEL
Made in
Germany
Certified Quality
MACHEREY-NAGEL
Forensic products
Consumables used in forensics need to be specially treated in order to deactivate
any PCR-amplifiable DNA. MACHEREY-NAGEL has a stringent and controlled
production process to avoid any contaminating PCR-detectable background.
MN products carrying the forensic quality seal contain plastic materials that are
ethylene oxide treated to inactivate any DNA.
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1

Transfection-grade plasmid DNA
transfection-grade
endotoxin-free
high- / low-copy plasmids
large constructs
desalting tool
NEW
NucleoBond ® Xtra BAC
Features
The 2 nd generation of anion exchanger for large construct plasmid DNA
• Optimized column design – one prep in approximately 75 min
• Column filter included – parallel lysate clearing and loading onto the column
• Optimal silica material – yields up to 150 μg
• Transfection-grade plasmid DNA using proven anion-exchange technology
• LyseControl for visualization of completed lysis / neutralization
Product at a glance
Technology
Anion-exchange chromatography
Format
Gravity-flow columns
Lysate clarification
Column filters
Sample material
250–750 mL
Vector size
< 300 kbp
Typical yield
10–150 μg
A 260 /A 280 1.80–1.95
Isopropanol precipitation Centrifugation
Preparation time
75 min/prep
Binding capacity
150 μg
Plasmid DNA
Applications
• Purification of P1, BACs, PACs, and other large constructs from E. coli
• Typical downstream applications: cloning, PCR, restriction analysis, sequencing, and transfection
For detailed product information and application data, see www.mn-net.com/plasmid
Ordering information
Product Preps Specification REF
NucleoBond ® Xtra BAC 10 NucleoBond ® Xtra BAC Columns
740436.10
with inserted NucleoBond ® Xtra Column Filters,
buffers, RNase A
25 as above 740436.25
Product accessories Pack of Specification REF
NucleoBond ® Xtra Combi Rack 1 for use with NucleoBond ® Xtra Midi, Maxi, BAC, 740415
AX 100, 500, 2 000, and 10 000 Columns
NucleoBond ® Xtra BAC Buffer Set 1 additional lysis buffers (1 L each):
740437
Buffers RES-BAC, LYS-BAC, NEU-BAC, and RNase A
For separate kit components, see “Accessories” page 137 of the main catalog Bioanalysis 2011 / 2012.
2
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Transfection-grade, endotoxin-free plasmid DNA
transfection-grade
endotoxin-free
high-copy plasmids
NEW
NucleoBond ® 96 Xtra EF
Features
High-throughput isolation of endotoxin-free plasmid DNA in 96-well format
• Plasmid DNA with less than 0.1 EU/μg for transfection of highly sensitive cells
• Patented endotoxin removal by additional washing step
• Processing of up to 5 mL bacterial culture
• NucleoBond ® Filter Plate for convenient filtration of bacterial lysates
• Processing under vacuum or by centrifugation
• Innovative MN Wash Plate minimizes risk of cross-contamination
• NucleoBond ® Finalizer Plate to speed up DNA precipitation
Product at a glance
Technology
Anion-exchange chromatography
Format
96-well plates
Processing
Manual
Lysate clarification
96-well filter plates
Sample material
1–5 mL E. coli culture
Vector size
< 15 kbp
< 300 kbp (without NucleoBond ® Finalizer Plate)
Typical yield
2–4 μg (1.5 mL LB / TB in 96-well culture plates)
10–50 μg (5 mL LB / TB in glass tubes)
A 260 /A 280 1.80–1.95
Elution volume
100–200 μL
Preparation time
120 min/plate
Binding capacity
50 μg
Applications
• Manual isolation of high-copy plasmids
• Typical downstream applications: transfection, cloning, sequencing, PCR, restriction analysis
Plasmid DNA
For detailed product information and application data, see www.mn-net.com/plasmid
Ordering information
Product Preps Specification REF
NucleoBond ® 96 Xtra EF 1 x 96 NucleoBond ® Xtra EF Plate, NucleoBond ® Finalizer 740430.1
Plate, NucleoBond ® Filter Plate, Culture Plate, MN
Wash Plate, Elution Plate U-bottom, Gas-permeable
Foil, Self-adhering Foil, buffers, RNase A,
4 x 96 as above 740430.4
Product accessories Pack of Specification REF
NucleoVac Vacuum Manifold 1 740681
NucleoVac Vacuum Regulator 1 for controlling of vacuum 740641
For separate kit components, see “Accessories” page 137 of the main catalog Bioanalysis 2011 / 2012.
www.mn-net.com 3

PCR clean-up and gel extraction
single prep
manual HTP
automated HTP
mini spin columns
silica matrix
Update
NucleoSpin ® Gel and PCR Clean-up
Formerly known as “NucleoSpin ® Extract II”
Features
PCR clean-up and gel extraction – the two-in-one kit with optimized recovery and elution volume
• Two applications, one kit – one buffer with optimal performance for both applications
• High recoveries for fragments down to 50 bp
• Minimized elution volume: 15 μL – highly concentrated DNA
• Binding buffer with pH indicator
• Separate buffers for single stranded DNA or SDS containing samples available
• Suitable for all gel buffer systems (e.g., TAE, TBE)
Clean-up
Product at a glance
Technology
Silica-membrane technology
Format
Mini spin columns
Sample material
< 400 μL PCR reaction mixture
< 400 mg TAE / TBE agarose gel
Fragment size
50 bp–approx. 20 kbp
Typical recovery 70–95 %
A 260 /A 280 1.8–1.9
Elution volume
15–30 μL
Preparation time
10 min/6 preps
Binding capacity
25 μg
Applications
• Purification of PCR products
• Extraction of DNA from agarose gels
• Support protocol for DNA extraction from polyacrylamide gels
• Support protocol for clean-up of single stranded DNA and support protocol for RNA extraction from agarose
gels (Binding Buffer NTC required, not included in the kit, see “Ordering information”)
• Support protocol for clean-up of SDS-containing samples (Binding Buffer NTB required, not included in the kit,
see “Ordering information”)
• Typical downstream applications: cloning, sequencing, PCR, restriction analysis
For detailed product information and application data, see www.mn-net.com/cleanup
Ordering information
Product Preps Specification REF
NucleoSpin ® Gel and PCR Clean-up 10 NucleoSpin ® Gel and PCR Clean-up Columns, 740609.10
Collection Tubes (2 mL), buffers
50 as above 740609.50
250 as above 740609.250
Product accessories Pack of Specification REF
Buffer NTB 150 mL for clean-up of SDS-containing samples 740595.150
Buffer NTC 100 mL for clean-up of single stranded DNA 740654.100
For separate kit components, see “Accessories” page 137 of the main catalog Bioanalysis 2011 / 2012.
4
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PCR clean-up
Update
single prep
manual HTP
automated HTP
8-well strips
96-well plates
96-well systems
NucleoSpin ® 8 / 96 PCR Clean-up
NucleoSpin ® 8 / 96 PCR Clean-up Core Kit
Formerly known as “NucleoSpin ® 8 / 96 Extract II”
Features
Clean-up of PCR products in flexible 8-well strip format and for high throughput
in convenient 96-well plate format
• Time-saving parallel clean-up of PCR products
• Complete removal of primers and primer-dimers
• Optimal recovery
• Suitable for processing under vacuum and centrifugation
• Innovative MN Wash Plate minimizes risk of cross-contamination
• Suitable for manual or automated use
• NucleoSpin ® 8 / 96 PCR Clean-up Core Kits: kits with basic content focused on automation platforms.
Additional accessories can be combined as needed.
Product at a glance
NucleoSpin ® 8 PCR Clean-up
NucleoSpin ® 8 PCR Clean-up Core Kit
NucleoSpin ® 96 PCR Clean-up
NucleoSpin ® 96 PCR Clean-up Core Kit
Technology
Silica-membrane technology
Format 8-well strips 96-well plates
Processing
Manual or automated, vacuum or centrifugation
Sample material
< 100 μL PCR reaction mixture
Fragment size 50 bp–10 kbp 50 bp–10 kbp
Typical recovery 75–95 % 75–95 %
A 260 /A 280 1.7–1.8 1.7–1.8
Elution volume 75–150 μL 75–150 μL
Preparation time 30 min/6 strips 45 min/plate
Binding capacity 15 μg 15 μg
Clean-up
Applications
• Manual or automated purification of PCR products
For detailed product information and application data, see www.mn-net.com/cleanup
Ordering information, see next page
www.mn-net.com 5

PCR clean-up
single prep
manual HTP
automated HTP
8-well strips
96-well plates
96-well systems
Ordering information
Product Preps Specification REF
NucleoSpin ® 8 PCR Clean-up 12 x 8 NucleoSpin ® PCR Clean-up Binding Strips, MN Wash 740668
Plate, Rack of Tube Strips, buffers
60 x 8 as above 740668.5
NucleoSpin ® 8 PCR Clean-up 48 x 8 NucleoSpin ® PCR Clean-up Binding Strips, buffers 740463.4
Core Kit
NucleoSpin ® 96 PCR Clean-up 1 x 96 NucleoSpin ® PCR Clean-up Binding Plate, MN Wash 740658.1
Plate, Elution Plate U-bottom, Self-adhering Foil, buffers
2 x 96 as above 740658.2
4 x 96 as above 740658.4
24 x 96 as above 740658.24
NucleoSpin ® 96 PCR Clean-up
Core Kit
4 x 96 NucleoSpin ® PCR Clean-up Binding Plates, buffers 740464.4
Product accessories Pack of Specification REF
NucleoVac 96 Vacuum Manifold 1 740681
NucleoVac Vacuum Regulator 1 for controlling of vacuum 740641
Starter Set A 1 for use of NucleoSpin ® 8-well strips on the NucleoVac 96 740682
Vacuum Manifold
Starter Set C 1 for use of NucleoSpin ® 8-well strips under centrifugation 740684
For separate kit components, see “Accessories” page 137 of the main catalog Bioanalysis 2011 / 2012.
Clean-up
6
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Genomic DNA clean-up
single prep
mini spin columns
mini spin columns – XS design
NEW
NucleoSpin ® gDNA Clean-up
Features
Post clean-up and concentration of large DNA fragments
• Effective post clean-up and concentration of large DNA fragments for successful
downstream applications
• High recovery rates up to 90 % for DNA fragments 100 bp–50 kbp
• Concentrate your DNA sample easily and faster compared to microdialysis filtrations units
• 15 min/10 preps – time-saving procedure
Product at a glance
Technology
Silica-membrane technology
Format
Mini spin columns
Sample material
< 150 μL aqueous DNA solution (< 25 μg DNA)
Fragment size
100 bp–approx. 50 kbp
Typical recovery 80–90 %
A 260 /A 280 1.8–1.9
Elution volume
50–100 μL
Preparation time
15 min/10 preps
Binding capacity
50 μg
Applications
• Clean-up and concentration of pre-purified DNA (organic extractions)
• Clean-up and concentration of DNA from enzymatic reactions
• Not recommended for gel or PCR clean-up! For these applications, please use NucleoSpin ® Gel and PCR
Clean-up (see page 4)
• Typical downstream applications: qPCR, enzymatic reactions, Southern blotting, STR amplification
Clean-up
For detailed product information and application data, see www.mn-net.com/cleanup
Ordering information
Product Preps Specification REF
NucleoSpin ® gDNA Clean-up 10 NucleoSpin ® gDNA Clean-up Columns,
740230.10
Collection Tubes (2 mL), buffers
50 as above 740230.50
250 as above 740230.250
For separate kit components, see “Accessories” page 137 of the main catalog Bioanalysis 2011 / 2012.
www.mn-net.com 7

Genomic DNA clean-up
single prep
mini spin columns
mini spin columns – XS design
NEW
NucleoSpin ® gDNA Clean-up XS
Features
Post clean-up and concentration of small amounts of large DNA fragments
• Efficient post clean-up of large DNA fragments for successful downstream applications
• Recommended for small DNA quantities extracted from, e.g., forensic samples
• Unique XS spin column for low elution volume (> 6 μL) – highly concentrated DNA
• Easier and faster than microdialysis filtration units
• 20 min/6 preps – time-saving procedure
Product at a glance
Technology
Silica-membrane technology
Format
Mini spin columns – XS design
Sample material
< 400 μL aqueous DNA solution (< 2 μg DNA)
Fragment size
100 bp–approx. 50 kbp
Typical recovery 60–70 %
A 260 /A 280 1.8–1.9
Elution volume
6–10 μL
Preparation time
20 min/6 preps
Binding capacity
3 μg
Clean-up
Applications
• Clean-up and concentration of pre-purified DNA (organic extractions)
• Clean-up and concentration of DNA from enzymatic reactions
• Not recommended for gel or PCR clean-up! For these applications, please use NucleoSpin ® Gel and PCR
Clean-up (see page 4)
• Typical downstream applications: PCR, enzymatic reactions, Southern blotting, qPCR, STR amplification
For detailed product information and application data, see www.mn-net.com/cleanup
Ordering information
Product Preps Specification REF
NucleoSpin ® gDNA Clean-up XS 10 NucleoSpin ® gDNA Clean-up XS Columns, 740904.10
Collection Tubes (2 mL), buffers
50 as above 740904.50
250 as above 740904.250
For separate kit components, see “Accessories” page 137 of the main catalog Bioanalysis 2011 / 2012.
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MicroRNA
single prep
mini spin columns
NEW
NucleoSpin ® miRNA Plasma
Features
Isolation of small RNA and DNA from plasma and serum
• Efficient and selective isolation of small RNA from plasma or serum samples
• Superior recovery of small RNA > 15 nt
• Optional on-column DNA digest for increased sensitivity in downstream applications
• Simple and fast procedure – without phenol / chloroform
Product at a glance
Technology
Format
Sample material
Fragment size
Elution volume
Preparation time
Binding capacity
Silica-membrane technology
Mini spin columns
300 μL plasma or serum (up to 900 μL with multiple loading)
< 1000 nt / bp
20–50 μL
40 min/10 preps (without rDNase digestion)
70 min/10 preps (with rDNase digestion)
200 μg
Applications*
• Isolation of small RNA from plasma or serum for cancer research (e.g., reliable monitoring of clinical parameters)
• Typical downstream applications: real-time qRT-PCR, chip hybridization
* Patent pending; kits to be used for research purposes only (see page 17)
For detailed product information and application data, see www.mn-net.com/RNA
RNA
Ordering information
Product Preps Specification REF
NucleoSpin ® miRNA Plasma 10 NucleoSpin ® miRNA Columns, Collection Tubes (2 mL), 740981.10
buffers
50 as above 740981.50
250 as above 740981.250
For separate kit components, see “Accessories” page 137 of the main catalog Bioanalysis 2011 / 2012.
www.mn-net.com
9

RNA from blood
single prep
manual HTP
automated HTP
mini spin columns
midi spin columns
NEW
NucleoSpin ® RNA Blood
Direct blood lysis –
no selective erythrocyte lysis
Features
Isolation of RNA from fresh or frozen whole blood (human or animal)
• Direct total blood lysis: very simple and convenient procedure (no selective erythrocyte lysis
at 4 °C – complete processing at room temperature)
• Superior RNA yield and quality from up to 400 μL whole blood
• Efficient on-column DNA removal for increased sensitivity in downstream applications
• Compatible with common blood collection tubes and anticoagulants (e.g., EDTA, citrate, and
heparin)
Product at a glance
Technology
Silica-membrane technology
Format
Mini spin columns
Sample material
< 400 μL whole blood (fresh or frozen)
Fragment size
> 200 nt
Typical yield
1–8 μg (400 μL whole blood)*
A 260 /A 280 1.9–2.1
Elution volume
40–120 μL
Preparation time
55 min/6 preps
Binding capacity
200 μg
* RNA yield strongly depends on the leukocyte number in each individual blood sample.
RNA
Applications**
• Isolation of RNA from fresh or frozen whole blood from common blood collection tubes (EDTA, citrate, heparin)
• Typical downstream applications: qRT-PCR, next generation sequencing, blotting, array technology
** Kits to be used for research purposes only (see page 17)
For detailed product information and application data, see www.mn-net.com/RNA
Ordering information
Product Preps Specification REF
NucleoSpin ® RNA Blood 10 NucleoSpin ® RNA Blood Columns with Collection Tubes, 740200.10
Collection Tubes (2 mL), buffers, RNase-free rDNase,
Liquid Proteinase K
50 as above 740200.50
For separate kit components, see “Accessories” page 137 of the main catalog Bioanalysis 2011 / 2012.
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RNA from blood
single prep
manual HTP
automated HTP
mini spin columns
midi spin columns
NEW
NucleoSpin ® RNA Blood Midi
Direct blood lysis –
no selective erythrocyte lysis
Features
Midi spin kit for the isolation of RNA from fresh or frozen blood (human or animal)
• Direct total blood lysis: very simple and convenient procedure (no selective erythrocyte lysis
at 4 °C – complete processing at room temperature)
• Superior RNA yield and quality from up to 1.3 mL whole blood
• Efficient on-column DNA removal for an increased sensitivity in downstream applications
• Compatible with common blood collection tubes and anticoagulants (e.g., EDTA, citrate, and
heparin)
Product at a glance
Technology
Silica-membrane technology
Format
Midi spin columns
Sample material
400–1300 μL whole blood (fresh or frozen)
Fragment size
> 200 nt
Typical yield
4–26 μg (1300 μL whole blood)*
A 260 /A 280 1.9–2.1
Elution volume
200–400 μL
Preparation time
75 min/6 preps
Binding capacity
700 μg
* RNA yield strongly depends on the leukocyte number in each individual blood sample.
Applications**
• Isolation of RNA from fresh or frozen whole blood from common blood collection tubes (EDTA, citrate, heparin)
• Typical downstream applications: qRT-PCR, next generation sequencing, blotting, array technology
RNA
** Kits to be used for research purposes only (see page 17)
For detailed product information and application data, see www.mn-net.com/RNA
Ordering information
Product Preps Specification REF
NucleoSpin ® RNA Blood Midi 20 NucleoSpin ® RNA Blood Midi Columns with Collection Tubes, 740210.20
Collection Tubes (15 mL), buffers, RNase-free rDNase,
Liquid Proteinase K
For separate kit components, see “Accessories” page 137 of the main catalog Bioanalysis 2011 / 2012.
www.mn-net.com 11

RNA from blood
NEW
single prep
manual HTP
automated HTP
8-well strips
96-well plates
NucleoSpin ® 8 / 96 RNA Blood
Direct blood lysis –
no selective erythrocyte lysis
Features
Isolation of total RNA from whole blood (human or animal) in flexible 8-well strip format
or proven 96-well format
• Direct total blood lysis: very simple and convenient procedure (no selective erythrocyte
lysis at 4 °C – complete processing at room temperature)
• Compatible with common blood collection tubes and anticoagulants (e.g., EDTA, citrate,
and heparin)
• Suitable for fresh and frozen blood samples
• rDNase included for efficient removal of genomic DNA
• Processing under vacuum or by centrifugation
• Suitable for manual and automated processing
• Innovative MN Wash Plate minimizes risk of cross-contamination
RNA
Product at a glance
NucleoSpin ® 8 RNA Blood NucleoSpin ® 96 RNA Blood
Technology
Silica-membrane technology
Format 8-well strips 96-well plates
Processing
Manual or automated, vacuum or centrifugation
Sample material
< 400 μL whole blood (fresh or frozen)
Fragment size > 200 nt > 200 nt
Typical yield 1–8 μg 400 μL (whole blood)* 1–8 μg 400 μL (whole blood)*
A 260 /A 280 1.9–2.1 1.9–2.1
Elution volume 50–130 μL 50–130 μL
Preparation time 60 min/6 strips 100 min/plate
Binding capacity 100 μg 100 μg
* RNA yield strongly depends on the leukocyte number in each individual blood sample.
Applications**
• Manual or automated isolation of total RNA from fresh or frozen whole blood stabilized with, for example,
EDTA, citrate, or heparin
• Typical downstream applications: qRT-PCR, next generation sequencing, blotting, array technology
** Kits to be used for research purposes only (see page 17)
For detailed product information and application data, see www.mn-net.com/RNA
12
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RNA from blood
single prep
manual HTP
automated HTP
8-well strips
96-well plates
Ordering information
Product Preps Specification REF
NucleoSpin ® 8 RNA Blood 12 x 8 NucleoSpin ® RNA Blood Binding Strips, MN Wash Plates, 740220
Square-well Blocks, Racks of Tube Strips, Elution Plates
U-bottom, Self-adhering Foil, buffers, RNase-free rDNase,
Liquid Proteinase K
60 x 8 as above 740220.5
NucleoSpin ® 96 RNA Blood 2 x 96 NucleoSpin ® RNA Blood Binding Plates, MN Wash Plates, 740225.2
Square-well Blocks, Round-well Blocks Low, Elution
Plates U-bottom, Self-adhering Foil, buffers, RNase-free
rDNase, Liquid Proteinase K
4 x 96 as above 740225.4
Product accessories Pack of Specification REF
NucleoVac 96 Vacuum Manifold 1 740681
NucleoVac Vacuum Regulator 1 for controlling of vacuum 740641
Starter Set A 1 for use of NucleoSpin ® 8-well strips on the NucleoVac 96 740682
Vacuum Manifold
Starter Set C 1 for use of NucleoSpin ® 8-well strips under centrifugation 740684
For separate kit components, see “Accessories” page 137 of the main catalog Bioanalysis 2011 / 2012.
RNA
www.mn-net.com 13

Viral RNA and DNA from serum and plasma
single prep
manual HTP
automated HTP
mini spin columns
mini spin columns, CE-marked
funnel columns
NEW
NucleoSpin ® Dx Virus
CE-IVD marked kit
Features
CE-IVD marked mini spin kit* for the isolation of viral RNA and DNA
• CE-marked in accordance with EU Directive 98/79/EC
• Fits into in-vitro diagnostic workflows
• Compatible with fresh or frozen human serum and plasma treated with EDTA or citrate
• Highly consistent viral RNA and DNA isolation for a broad range of viral titers
• Also suitable for animal samples
* CE-IVD marked kit: not available in all countries, please inquire.
Product at a glance
Technology
Format
Sample material
Fragment size
Elution volume
Preparation time
Binding capacity
Silica-membrane technology
Mini spin columns
150 μL plasma or serum (fresh or frozen, EDTA or citrate treated)
100 bp–approx. 50 kbp
50 μL
30 min/6 preps
40 μg
Viral RNA and DNA
Applications
• Isolation of viral RNA from 150 μL human plasma or serum
• Isolation of viral DNA from 150 μL human plasma or serum
• Isolation of viral RNA and viral DNA from 150 μL human plasma or serum (simultaneous isolation)
• Isolation of viral RNA and / or viral DNA from animal samples, swabs, plasma, or serum
(out of the scope of the IVD Directive 98/79/EC).
• Typical downstream applications: enzymatic amplification and detection of viral RNA and / or viral DNA
(e.g., PCR, RT-PCR)
For detailed product information and application data, see www.mn-net.com/virus
Ordering information
Product Preps Specification REF
NucleoSpin ® Dx Virus 50 NucleoSpin ® Dx Virus Columns with Collection Tubes, Lysis Tubes 740895.50
(1.5 mL), Collection Tubes (2 mL), Elution Tubes (1.5 mL), buffers,
Proteinase K, Carrier RNA
For separate kit components, see “Accessories” page 137 of the main catalog Bioanalysis 2011 / 2012.
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Purification of His-tag proteins
NEW
bulk material
single prep
manual HTP
automated HTP
96-well plates
Protino ® 96 Ni-NTA
Features
High-throughput purification of His-tag proteins
• Efficient protein purification using chelating group NTA (nitrilotriacetic acid)
• Unique Protino ® Purification Plate for leak-free incubation during all steps
• Plate outlet and MN Wash Plate prevent cross-contamination
• High well-to-well reproducibility
• High capacity and high affinity
• Purification under native and denaturing conditions
• Processing under vacuum or by centrifugation
Product at a glance
Technology
IMAC (immobilized metal ion affinity chromatography)
Chelating ligand
NTA (nitrilotriacetic acid)
Format
96-well plates (Protino ® Purification Plate with special filter frit)
Matrix 6 % beaded agarose (cross-linked), precharged with Ni 2+ ,
Volume capacity per well
1.4 mL
Recommended sample volume < 750 μL/well, supplied matrix is sufficient for 96 preps with 50 μL
of settled agarose beads per well
Bed volume
Variable (50 μL recommended)
Binding capacity*
< 2 mg per well when using 50 μL of settled agarose
* Binding capacity will vary for each polyhistidine-tagged protein and strongly depends on chromatographic conditions.
Applications
• Purification of polyhistidine-tagged proteins
• Batch binding experiments
• Screening experiments to optimize chromatographic conditions
Protein
For detailed product information and application data, see www.mn-net.com/protein
Ordering information
Product Preps Specification REF
Protino ® 96 Ni-NTA 1 x 96 Protino ® Ni-NTA Agarose, Protino ® Purification Plate, 745425.1
MN Wash Plate
4 x 96 as above 745425.4
Product accessories Pack of Specification REF
Protino ® Purification Plate 1 96-well plate, suitable for centrifugation and vacuum 745426.1
4 as above 745426.4
Receiver Plate 10 μm 4 96-well plate with an inserted filter frit of 10 μm pore size, 740989.4
suitable for centrifugation and vacuum
NucleoVac 96 Vacuum Manifold 1 740681
NucleoVac Vacuum Regulator 1 for controlling of vacuum 740641
MN Shaker Frame 1 shaking frame for Protino ® Purification Plate 740489
For separate kit components, see “Accessories” page 137 of the main catalog Bioanalysis 2011 / 2012.
www.mn-net.com 15

Sterile filtration
Update
auxiliary tools
blotting
blood sample storage
sterile filtration
decontamination of EtBr
surface protection
MN Sterilizer CA
Features
Highest quality syringe filter for fast and efficient sterile filtration of aqueous solutions
• Removal of particulates > 0.2 μm
• Fast flow of liquid through the filter
• Very robust membrane – high stability
• Low protein binding
Auxiliary tools and transfer media
Product at a glance
Format
Syringe filter units
Housing material
Methyl methacrylate-butadiene-styrene polymer (MBS)
Membrane material
Cellulose acetate
Nominal pore size
0.2 μm
Filter diameter
28 mm
Effective filtration area 6.2 cm 2
Hold-up volume (after air purge) 150 μL
Inlet connection
Female luer lock
Outlet connection
Male slip luer
Maximum pressure
4.5 bar
Maximum temperature 50 °C
Sterilization method
Pre-sterilized with ethylene oxide
Applications
• Sterile filtration and removal of particulates from aqueous solutions
For detailed product information, see www.mn-net.com/auxiliary
Ordering information
Product Pack of Specification REF
MN Sterilizer CA 50 Disposable filter units, membrane material CA (cellulose acetate),
pore size 0.2 μm, membrane diameter 28 mm
740401.50
16
www.mn-net.com

Trademarks • Product use restriction
Trademarks used in this supplement
MACHEREY-NAGEL trademarks
NucleoBond
NucleoSpin
Protino
kits and cartridges for nucleic acid purification (anion-exchange chromatography)
kits for purification of DNA and RNA (silica-membrane technology)
kits for purification of recombinant proteins (affinity chromatography)
Trademarks of other companies
Registered trademarks
TRIzol Molecular Research Center, Inc.
PCR is patented by Hoffmann La Roche
Disclaimer
All used names and denotations can be brands, trademarks, or registered labels of their respective owner – also if they do not have a
special denotation. To mention products and brands is only a kind of information, i.e., it does not offend against trademarks and brands
and can not be seen as a kind of recommendation or assessment. Regarding these products or services we can not grant any guarantees
regarding selection, efficiency, or operation.
Product use restriction / Warranty
MACHEREY-NAGEL Bioanalysis products and their single components are intended, developed, designed, and sold FOR RESEARCH
PURPOSES ONLY, except, however, any other function of the product being expressly described in original MACHEREY-NAGEL product
leaflets.
MACHEREY-NAGEL products are intended for GENERAL LABORATORY USE ONLY!
MACHEREY-NAGEL products are suited for QUALIFIED PERSONNEL ONLY!
MACHEREY-NAGEL products shall in any event only be used wearing adequate PROTECTIVE CLOTHING.
For detailed information please refer to the respective Material Safety Data Sheet of the product!
MACHEREY-NAGEL products shall exclusively be used in an ADEQUATE TEST ENVIRONMENT.
MACHEREY-NAGEL does not assume any responsibility for damages due to improper application of our products in other fields of application.
Application on the human body is STRICTLY FORBIDDEN. The respective user is liable for any and all damages resulting from such
application.
DNA/RNA/PROTEIN purification products of MACHEREY-NAGEL are suitable for IN-VITRO-USES ONLY! ONLY MACHEREY-NAGEL
products specially labeled as IVD are also suitable for IN-VITRO-diagnostic use. Please pay attention to the package of the product. IN-
VITRO-diagnostic products are expressly marked as IVD on the packaging.
IF THERE IS NO IVD SIGN, THE PRODUCT SHALL NOT BE SUITABLE FOR IN-VITRO-DIAGNOSTIC USE!
ALL OTHER PRODUCTS NOT LABELED AS IVD ARE NOT SUITED FOR ANY CLINICAL USE (INCLUDING, BUT NOT LIMITED TO
DIAGNOSTIC, THERAPEUTIC, AND/OR PROGNOSTIC USE).
No claim or representations are intended for its use to identify any specific organism or for clinical use (included, but not limited to diagnostic,
prognostic, therapeutic, or blood banking). It is rather in the responsibility of the user or – in any case of resale of the products – in
the responsibility of the reseller to inspect and assure the use of the DNA/RNA/protein purification products of MACHEREY-NAGEL for a
well-defined and specific application.
MACHEREY-NAGEL shall only be responsible for the product specifications and the performance range of MN products according to the
specifications of in-house quality control, product documentation, and marketing material.
For detailed information on product use restriction and warranty please refer to the respective user manuals.
Trademarks • Product use restriction
www.mn-net.com
17

DNA, RNA, and protein purification
Catalog supplement 2013
Service Bioanalysis
Technical Support and Customer Service phone +49 (0) 24 21 969-270
e-mail tech-bio@mn-net.com
Product Management phone +49 (0) 24 21 969-271
+49 (0) 24 21 969-275
e-mail pm-bio@mn-net.com
Sales and Marketing phone +49 (0) 24 21 969-272
+49 (0) 24 21 969-273
e-mail sales@mn-net.com
Business Development phone +49 (0) 24 21 969-272
e-mail bdm-bio@mn-net.com
Your local distributor
KATEN300039 Catalog Supplement en2/15/0/09.2012 PD
Printed in Germany
www.mn-net.com
MACHEREY-NAGEL
EN ISO 9001: 2008
CERTIFIED
MACHEREY-NAGEL GmbH & Co. KG · Neumann-Neander-Str. 6–8 · 52355 Düren · Germany
Germany
and international:
Switzerland:
MACHEREY-NAGEL AG
France:
MACHEREY-NAGEL EURL
Tel.: +49 24 21 969-0 Tel.: +41 62 388 55 00 Tel.: +33 388 68 22 68
Fax: +49 24 21 969-199 Fax: +41 62 388 55 05 Fax: +33 388 51 76 88
E-mail: info@mn-net.com E-mail: sales-ch@mn-net.com E-mail: sales-fr@mn-net.com
USA:
MACHEREY-NAGEL Inc.
Tel.: +1 484 821 0984
Fax: +1 484 821 1272
E-mail: sales-us@mn-net.com