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•Sigma factor changes the DNAbinding properties of RNA polymerase so that its affinity for general DNA is reduced and its affinity for promoters is increased. B-8
• An open complex describes the stage of initiation of transcription when RNA polymerase causes the two strands of DNA to separate to form the "transcription bubble". • Tight binding of RNA polymerase to DNA describes the formation of an open complex (when the strands of DNA have separated). •The ternary complex in initiation of transcription consists of RNA polymerase and DNA and a dinucleotide that represents the first two bases in the RNA product. • Abortive initiation describes a process in which RNA polymerase starts transcription but terminates before it has left the promoter. It then reinitiates. Several cycles may occur before the elongation stage begins. B-9
Page 1 and 2: Transcription Chapter 09 By Rasul C
Page 3 and 4: Bacterial transcription cycle Sigma
Page 5 and 6: Functioning of RNA polymerase. •
Page 7: Bacterial RNA Polymerase •The RNA
Page 11 and 12: An arrested RNA polymerase can rest
Page 13 and 14: Sigma factor controls binding to DN
Page 15 and 16: Promoter efficiencies can be increa
Page 17 and 18: RNA polymerase binds to one face of
Page 19 and 20: Substitution of sigma factors may c
Page 21 and 22: B-21
Page 23 and 24: The 2.4 helix of sigma determines s
Page 25 and 26: Early genes are transcribed before
Page 27 and 28: A cascade is a sequence of events,
Page 29 and 30: σF is inhibited by an anti-sigma f
Page 31 and 32: Terminators Intrinsic terminators a
Page 33 and 34: Action of rho A rho hexamer translo
Page 35 and 36: Immediate early phage genes in phag
Page 37 and 38: Antitermination lies upstream in th
Page 39 and 40: • Antitermination occurs in the r
Page 41 and 42: Typically located at -25 region sub
Page 43 and 44: Large subunit of RNA Pol has 7 amin
Page 45: Prokaryotic RNA Pol= 5 subunits Euk

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