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P1 A Study on the possibility to increase the antioxidant status of the plant food G. Tchoupakhina, L. Skrypnik Immanuel Kant State University of Russia 236040 Russia, Kaliningrad, Universitetskaya str., 2, skr_sun@mail.ru Antioxidants are substances that oppose the damaging effects of free radicals that are generated as a result of both the normal byproduct of metabolism as well as those generated through the exposure to environmental factors such as cigarette smoke, sunrays, pollution, etc. Antioxidants are vitamins, minerals and nutrients as well as enzymes created by the organism to counter the effects of oxidation and free radicals. By countering the effects of free radicals, antioxidants play a role in preventing chronic diseases and the aging process. However, the accumulation of antioxidants by plants strongly depends on the conditions of growth as plants sensitively react to changes in conditions such as light intensity, mineral availability, temperature and water availability. The purpose of this work was the study of the action of different factors (intensity of light and mineral nutrition) on the accumulation of some low-molecular antioxidants by barley plants. For analyses were used 10 days after sowing barley plants (Hordeum vulgare L.), grown as the hydroponic culture. Plants were cultivated on Hogland’s nutrient solution with and without selenium. Selenium was added in form of sodium selenate in the concentration of 1 mol L -1 . The barley plants with different selenium nutrition were used by the study the effect of the light intensity (175 μmol m -2 s -1 and 500 μmol m -2 s -1 ) on the accumulation of some low-molecular antioxidants (carotenoids, anthocyans, rutin, glutathione, riboflavin, ascorbic acid) by the plants. The findings show the positive effect of selenium on the accumulation of some low-molecular antioxidants. In the barley plants by the addition of selenium to nutrient solution increased the content of glutathione by 15%, ascorbic acids by 12%, rutin by 6%. The content of carotenoids and riboflavin changed not significantly. A reduction of the content of anthocyans (to 29%) by the addition of selenium to nutrient solution can be also the proof of the positive action of selenium in the barley plants. It is known that the level of anthocyans is increased under the influence on the plants of a whole series of stress factors. Studies of the influence of high light intensity on the accumulation of some antioxidants in the Sedeficient plants showed that the exposure of sprouts on the light with intensity of 500 μmol m -2 s -1 during 12 hours enhanced the content of all analyzed low-molecular antioxidants (carotenoids on 11%, rutin on 17%, glutathione on 17%, anthocyans on 15%, riboflavin on 24%). The increase in the content of ascorbic acid was most significant - it was more than on 50%. Effect of high light intensity on the accumulation of some antioxidants in the Se-sufficient plants was different. The level of glutathione changed not significant in Se-sufficient plants after 12 h exposure on the high light intensity, while the content of ascorbic acid decreased by the same conditions up to 80 %. Thus, the results of investigating the influence of the light intensity and micronutrient selenium on the accumulation of low-molecular antioxidants (carotenoids, rutin, glutathione, anthocyans, ascorbic acid, and riboflavin) by the barley plants prove the possibility of increasing the antioxidant status of plant food through the changing of conditions of plant cultivation.
The antioxidative activity of the most frequently consumed Polish mushrooms Robaszkiewicz A., Bartosz G., Soszyński M. Department of Molecular Biophysics, University of Łódź, Banacha 12/16, 90-231 Łódź, POLAND P2 One of the nutritional benefits of mushrooms is the presence of bioactive secondary metabolites which are reported to reveal therapeutic effects in vivo. There is a lot of evidence that these metabolites display antioxidative properties. Therefore we selected the most frequently consumed species in Poland (Boletus edulis young – “white” and adult – “yellow”, Leccinum spp., Xerocomus bodius, Xerocomus subtomentosus, Cantharellus cibarius, Suillus spp., Suillus variegatus, Suillus bovinus, Pleurotus ostreatus, Agaricus bisporus) and determined the content of polyphenols and flavonoids, β-carotene, lycopene in aqueous and methanolic extracts of dried bodies as well as the reducing power and ability to scavenge ABTS •+ . Aqueous extracts of Boletus edulis “white” and Xerocomus subtomentosus displayed the highest total content of phenolics, however the highest concentration of flavonoids was found for Xerocomus bodius. The quantity of polyphenols and flavonoids in methanolic extracts was lower than in the aqueous extracts except for the case of Suillus bovinus. We observed a strong correlation (r>0.9) between the concentration of polyphenols and reducing power and scavenging effects in both aqueous and methanolic extracts. The weakest effects were noticed for Agaricus bisporus, Pleurotus ostreatus and Cantharellus cibarius. The highest content of β-carotene was found in methanolic extracts of Suillus spp., Suillus variegates and Suillus bovinus, while the amount of lycopene was the highest for Suillus bovinus. Mushrooms selected for our experiments were found to differ in the content of antioxidants and total antioxidative capacity.
Page 1 and 2: 3 ème Symposium International Nutr
Page 3 and 4: PROGRAMME SCIENTIFIQUE SCIENTIFIC P
Page 5 and 6: l’exercice Regulation of inflamma
Page 7 and 8: 12h 15 - 13h 15 Modérateurs 13h 15
Page 9 and 10: Oral Presentations
Page 11 and 12: Vina J, Borras C, Gomez-Cabrera MC
Page 13 and 14: PL3 Micronutrients: an update on fl
Page 15 and 16: OP2 Physical exercise and oxidative
Page 17 and 18: OP4 Studying the dietary modulation
Page 19 and 20: OP6 Redox regulation of loss of mus
Page 21 and 22: OP8 The consequences of physical in
Page 23 and 24: OP10 Endurance training failed to s
Page 25 and 26: OP12 Bowman-Birk inhibitor concentr
Page 27 and 28: OP14 Resveratrol regulates DDAH act
Page 29 and 30: OP16 How mitochondria protect again
Page 31 and 32: OP18 Cardioprotection and longevity
Page 33 and 34: N-3 long chain polyunsaturated fatt
Page 35 and 36: OP22 Effects of diabetes and exerci
Page 37 and 38: OP24 The energy-redox axis in aging
Page 39 and 40: OP26 Caloric restriction and exerci
Page 41 and 42: OP27 Oxidized protein degradation a
Page 43 and 44: Shenkin A. Serum Prealbumin: Is It
Page 45: Posters
Page 49 and 50: P4 Bioavailability of QH absorb, a
Page 51 and 52: Tamoxifen and raloxifen in the prot
Page 53 and 54: P8 Oxidant-antioxidant balance in n
Page 55 and 56: The antioxidant status of water ext
Page 57 and 58: P11 Lipoperoxyl radical scavenging
Page 59 and 60: Measurement of the antioxidant enzy
Page 61 and 62: P15 Using of Cyclic Voltammetry in
Page 63 and 64: P17 Oxidized nucleic acid bases as
Page 65 and 66: P19 Vitamin and mineral supplementa
Page 67 and 68: P21 Gestation, training and redox s
Page 69 and 70: P23 Effect of fermented papaya prep
Page 71 and 72: P25 Antioxidant status, hormones an
Page 73 and 74: P27 Palmitic acid stimulates nitric
Page 75 and 76: P29 New in vitro screening tools to
Page 77 and 78: P31 Cardiovascular complications an
Page 79 and 80: P33 The modality of supplementation
Page 81 and 82: P35 Farnesol-mediated chemopreventi
Page 83 and 84: P37 Anticancer activity of dendrime
Page 85 and 86: P39 Pyrroline and pyrrolidine nitro
Page 87 and 88: P41 Changes of blood platelets apop
Page 89 and 90: P43 Apoptotic effects of genistein
Page 91 and 92: P45 Cytotoxicity and apoptosis in r
Page 93 and 94: P47 High molecular weight hyalurona
Page 95 and 96: P49 Importance of the spectrofluori
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P51 Antioxidants, trace elements an
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P53 Saturated fatty acid-induced in
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P55 Chronic treatment with tocotrie
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Tobacco smoke and UV light damage o
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P59 Oxidative stress in pulmonology
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P61 Oxidative stress markers in sen
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P63 Plasma proteins are specificall
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P65 In vitro Anti-inflammatory Acti
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Author index
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Brzuskiewicz J. ...................
Page 117 and 118:
Hartwich J. .......................
Page 119 and 120:
Mili A.............................
Page 121 and 122:
Sultan A. .........................
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Alleva Renata IRCCS Research Hospit
Page 125 and 126:
Brooks George University of Califor
Page 127 and 128:
Davies Kelvin J.A. University of So
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Pediatric Department Neonatal scree
Page 131 and 132:
Fax: 48(42)6354573 kgwozdz@biol.uni
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Istambul TURKEY Tel: +902164144733
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Linares-Vidal Victoria Laboratory o
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Mouithys-Mickalad Ange University o
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Fax: +41 61 815 8150 daniel.raeders
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6-20-2 Shinkawa, Mitaka Tokyo 181-8
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Valacchi Giuseppe University of Sie
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PROGRAMME SCIENTIFIQUE - Oxygen Club of California

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