a Whole Genome Array Approach - Jacobs University
a Whole Genome Array Approach - Jacobs University
a Whole Genome Array Approach - Jacobs University
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Ecological Aspects<br />
of the Marine Planctomycete Rhodopirellula baltica SH1 T<br />
- a <strong>Whole</strong> <strong>Genome</strong> <strong>Array</strong> <strong>Approach</strong> -<br />
by<br />
Dipl. Biol. Patricia Wecker<br />
A thesis submitted in partial fulfilment<br />
of the requirements for the degree of<br />
Doctor of Philosophy<br />
in Microbiology<br />
Approved, Thesis Committee<br />
Prof. Dr. Frank Oliver Glöckner (Chair)<br />
Prof. Dr. Matthias Ullrich (2 nd Reviewer)<br />
Prof. Dr. Rudolph Amann (3 rd Reviewer, External)<br />
Prof. Dr. Georgi Muskhelisvili (4 th Reviewer)<br />
Date of Defence: May 20, 2009<br />
SCHOOL OF ENGINEERING AND SCIENCE
I looked down. Descent was totally unappetizing……<br />
Too much labour, too many sleepless nights,<br />
and too many dreams had been invested to bring us this far.<br />
We couldn’t come back for another try next weekend.<br />
To go down now, even if we could have,<br />
would be descending to a future marked by one huge question:<br />
what might have been?<br />
Thomas F. Hornbein Everest: The West Ridge<br />
2
Background<br />
3
THESIS ABSTRACT<br />
Since the first microbial genome has been finished in the year 1995 more than 800 sequenced<br />
microorganisms are publicly available and many more are on their way. Sophisticated<br />
bioinformatic (in silico) analysis of the sequence information is required to process this huge<br />
amount of data and to generate sound hypotheses in terms of physiology and ecology of an<br />
organism as well as to efficiently screen for candidate genes for biotechnological relevant<br />
enzymes. Current success to directly obtain sequence information from the environment<br />
without former cultivation (the metagenomic approach) even speeds up the process. The most<br />
prominent in the marine field being the Venter Sargasso Sea expedition in 2004 delivering<br />
more than 1.2 million new genes. The introduction of new sequencing technologies, such as<br />
pyrosequencing will soon enable biologists to perform sequencing on a daily basis.<br />
However, high-throughput lab-methods, implemented by the DNA microarray technology, are<br />
more than ever required for the subsequent verification of initial bioinformatic findings and<br />
hypotheses. Post-genomic approaches like DNA microarrays have revolutionised molecular<br />
biology by enabling a new dimension of parallelism in expression profiling.<br />
Eight years ago, under the light of the up-coming cutting-edge technologies of the genomic<br />
era, the Max Planck Institute for Marine Microbiology in Bremen has initiated a genomics<br />
project for the complete genome sequencing and analysis of environmental relevant marine<br />
bacteria. Rhodopirellula baltica SH 1 T was selected as a marine representative of the globally<br />
distributed and environmentally important bacterial phylum Planctomycetales. It is a model<br />
organism for aerobic carbohydrate degradation in marine systems, where polysaccharides<br />
represent the dominant components of biomass.<br />
The complete genome sequence of R. baltica has been determined and functional annotation<br />
was performed. However, pure in silico predictions left about 55% of the genes without<br />
functional assignment. The availability of the whole genome sequence has already stimulated<br />
a set of follow-up studies ranging from proteomics to protein crystallisation as well as<br />
biotechnological applications.<br />
This work presents the establishment of, and the results gained with, the first whole genome<br />
microarray for a Planctomycete. It shows that massive expression profiling under defined<br />
environmental conditions can take the challenge to bridge the gap between classical studies on<br />
the cell biology and physiology of Planctomycetes and their active genetic repertoire.<br />
4
Background<br />
TABLE OF CONTENT<br />
1 BACKGROUND ............................................................................................................................. 1<br />
1.1 MARINE MICROBIAL ECOLOGY AND THE GENOMIC REVOLUTION............................................ 1<br />
1.2 PLANCTOMYCETES......................................................................................................................... 2<br />
1.2.1 RHODOPIRELLULA BALTICA .......................................................................................................... 4<br />
1.3 FURTHER OUTCOME OF THE GENOME ANNOTATION AND ITS LIMITATIONS............................ 9<br />
1.4 GENE EXPRESSION PROFILING BY DNA MICROARRAY ANALYSIS .......................................... 10<br />
1.5 PRINCIPLE OF DNA MICROARRAY ANALYSIS........................................................................... 11<br />
1.6 MICROARRAY DATA ANALYSIS .................................................................................................. 14<br />
1.7 MICROARRAY DATA MANAGEMENT.......................................................................................... 16<br />
2 RESEARCH AIMS....................................................................................................................... 17<br />
2.1 THE RHODOPIRELLULA BALTICA LIFE-CYCLE: GROWTH-DEPENDENT EXPRESSION PROFILING<br />
OF THE COMPLETE GENOME WITH A CDNA-BASED MICROARRAY .................................................. 17<br />
2.2 EXPRESSION PROFILING OF THE SULPHATASES GENES OF THE PLANCTOMYCETE<br />
RHODOPIRELLULA BALTICA GROWN ON DIFFERENT SULPHATED POLYSACCHARIDES .................... 18<br />
2.3 DESIGN OF THE WHOLE GENOME ARRAY OF RHODOPIRELLULA BALTICA .............................. 20<br />
3 RESULTS AND DISCUSSION ................................................................................................... 21<br />
3.1 TRANSCRIPTIONAL RESPONSE OF THE MODEL PLANCTOMYCETE RHODOPIRELLULA BALTICA<br />
SH1 T TO CHANGING ENVIRONMENTAL CONDITIONS......................................................................... 23<br />
3.2 THE RHODOPIRELLULA BALTICA LIFE-CYCLE: GROWTH-DEPENDENT EXPRESSION PROFILING<br />
OF THE COMPLETE GENOME WITH MICROARRAYS ........................................................................... 55<br />
3.3 HIGHLY ENANTIOSELECTIVE SEC-ALKYL SULFATASE ACTIVITY OF THE MARINE<br />
PLANCTOMYCETE RHODOPIRELLULA BALTICA SHOWS RETENTION OF CONFIGURATION............... 89<br />
3.4 FOSMIDS OF NOVEL MARINE PLANCTOMYCETES FROM THE NAMIBIAN AND OREGON COAST<br />
UPWELLING SYSTEMS AND THEIR CROSS-COMPARISON WITH PLANCTOMYCETE GENOMES ......... 90<br />
4 SUMMARY ................................................................................................................................... 91<br />
4.1 TRANSCRIPTIONAL RESPONSE OF THE MODEL PLANCTOMYCETE RHODOPIRELLULA BALTICA<br />
SH1 T TO CHANGING ENVIRONMENTAL CONDITIONS......................................................................... 92<br />
5
4.2 THE RHODOPIRELLULA BALTICA LIFE-CYCLE: GROWTH-DEPENDENT EXPRESSION PROFILING<br />
OF THE COMPLETE GENOME WITH MICROARRAYS ........................................................................... 93<br />
4.3 HIGHYL ENANTIOSELECTIVE SEC-ALKYL SULFATASE ACTIVITY OF THE MARINE<br />
PLANCTOMYCETE RHODOPIRELLULA BALTICA SHOWS RETENTION OF CONFIGURATION............... 94<br />
4.4 FOSMIDS OF NOVEL MARINE PLANCTOMYCETES FROM THE NAMIBIAN AND OREGON COAST<br />
UPWELLING SYSTEMS AND THEIR CROSS-COMPARISON AND THEIR CROSS-COMPARISON WITH<br />
PLANCTOMYCETE GENOMES ............................................................................................................... 95<br />
5 ESTABLISHING THE MICROARRAY PIPELINE................................................................ 96<br />
6 CONTRIBUTION TO THE RESEARCH ON PLANCTOMYCETES..................................... 98<br />
7 OUTLOOK.................................................................................................................................. 100<br />
8 REFERENCE.............................................................................................................................. 102<br />
9 ACKNOWLEDGEMENT.......................................................................................................... 109<br />
10 ANNEX ...................................................................................................................................... 111<br />
6
Background<br />
1 BACKGROUND<br />
1.1 Marine microbial ecology and the genomic revolution<br />
Modern marine microbial ecology was born in the 1970`s, when it was shown that bacteria<br />
were very abundant in the oceans and that most marine respiration was in the bacterial size<br />
fractions (Pedros-Alio 2006). Before then microbes were not even considered in the ecology<br />
of the oceans. Today we know that marine microbes are present at billions of cells per litre in<br />
seawater and that they play a key role in ocean ecosystems (DeLong and Karl 2005). It is<br />
therefore of crucial importance to understand microbial roles in oceanic primary production,<br />
the global carbon cycle and functioning of the biosphere.<br />
Microbiologists have been advancing their knowledge over the last 30 years through a<br />
progression of approaches that include taxonomic and physiological studies of cultivatable<br />
isolates, as well as cultivation-independent, rRNA-based phylogenetic survey techniques and<br />
genetic approaches. The invention of genome-based technologies (genomics) in the 1990´s<br />
revolutionised microbial ecology. The technological power of high-throughput sequencing<br />
and functional genomics enabled the study of genetic complexity of organisms at the genome<br />
level, while genetics had before focused on limited numbers of genes and gene products<br />
(Dupont et al. 2007). Microbiologists are now facing a genomic “data flood” with more than<br />
900 completed bacterial or archaeal genome sequences available and over 3000 more in<br />
progress (Thomas et al. 2007).<br />
With this, a new scientific discipline was created in marine microbial ecology, “marine<br />
ecological genomics” that studies the structure and functioning of a genome with the aim to<br />
understand the relationship between the organism and its biotic and abiotic environments.<br />
<strong>Approach</strong>es include (1) whole genome sequencing of key organisms and (2) genomic analysis<br />
of natural communities to understand how biodiversity supports ecosystem function<br />
(metagenomics). However, even though a given genome represents the blueprint of life for an<br />
organism, there is a need for functional analysis at the transcriptional and proteomic levels in<br />
order to define which of (1) the predicted genes can be expressed in principle, and (2) which<br />
physiological condition(s) induce their expression. In sharp contrast to the numerous<br />
publications of microbial genome sequences, several transcriptome and proteome studies have<br />
been reported so far (Zhou 2003).<br />
Already in the year 2000, the Department of Molecular Ecology at the Max Planck Institute<br />
for Marine Microbiology has initiated a marine environmental genomics project – the real<br />
1
Background<br />
environmental genomics-project (REGX). REGX aims at understanding the adaptations of<br />
marine bacteria to changing environmental conditions. Two sulphate reducing bacteria<br />
(i.e.: Desulfotalea psychrophila and Desulfobacterium autotrophicum) and one Planctomycete<br />
(Rhodopirellula baltica SH1 T ) were selected for whole genome sequencing, annotation and<br />
functional analysis. The successful sequencing and annotation of the whole genome of the<br />
Planctomycete R. baltica in 2003 (Glöckner et al. 2003) has established the framework for<br />
further functional genomic analysis.<br />
1.2 Planctomycetes<br />
Planctomycetes are a group of budding, peptidoglycan-less bacteria with increasing<br />
significance for microbial evolution, ecology, cell biology and genomics (Fuerst 1995). The<br />
phylum consists of a single family (Planctomycetaceae) with six accepted (Rhodopirellula,<br />
Blastopirellula, Pirellula, Planctomyces, Isosphaera, Gemmata) (Schlesner et al. 2004) and<br />
four candidate genera (Kuenenia, Brocadia, Scalindua, Anammoxoglobus). Latest studies<br />
group Planctomycetes with the PVC super phylum. This monophyletic clade is formed<br />
besides the Planctomycetes by the phyla Chlamydiae, Verrrucomicrobia, Lentisphaerae and<br />
the candidate phyla ‘Poribacter’and OP3 (Wagner and Horn 2006).<br />
Most cultured strains so far have been isolated from terrestrial, freshwater and marine habitats<br />
(Schlesner 1994; Fuerst 1995; Wang et al. 2002). In addition, studies based on cultivationindependent<br />
molecular methods, e.g. Fluorescence in situ Hybridisation (Amann and Ludwig<br />
2000), repeatedly confirmed the occurrence of Planctomycetes in terrestrial and aquatic<br />
habitats (DeLong and Franks 1993; Chatzinotas et al. 1998; Neef et al. 1998; Vergin et al.<br />
1998; Crump et al. 1999) and revealed an even broader distribution of the Planctomycetes in<br />
the environment, e.g. in hot springs (Giovannoni et al. 1987), marine sponges (Fuerst et al.<br />
1998; Fuerst et al. 1999; Pimentel-Elardo et al. 2003) as well as in hepatopancreas of the<br />
crustacean Panaeus monodon (Fuerst et al. 1991; Fuerst et al. 1997). This shows the<br />
successful colonisation of a broad spectrum of ecological niches by members of this phylum.<br />
Further surveys reported the presence of Planctomycetes in marine-snow particles (Rath<br />
1998) as well as in shallow and deep sea sediments of marine systems (Llobet-Brossa et al.<br />
1998; Inagaki et al. 2006; Kirkpatrick et al. 2006; Musat et al. 2006). Morris and coworkers<br />
showed that Planctomycetes appear and disappear with the blooming of diatoms (Morris et al.<br />
2006). They postulate a direct interaction and carbon flow between the algae and<br />
Planctomycetes. In addition, growth studies with R. baltica and other group members revealed<br />
2
Background<br />
their nutritional specialisation in aerobic carbohydrate utilisation (Schlesner et al. 2004),<br />
which points an important role in the global carbon cycle (Glöckner et al. 2003).<br />
In terms of cell biology all members of this group share several morphologically unique<br />
properties, such as a peptidoglycan-free proteinaceous cell wall (König et al. 1984; Liesack<br />
et al. 1986), intracellular compartmentalisation (Lindsay et al. 1997; Lindsay et al. 2001) and<br />
a mode of reproduction via budding which results in a cell-cycle that is characterised by<br />
motile and sessile morphotypes and resembles that known from Caulobacter crescentus<br />
(Tekniepe et al. 1981; Fuerst 1995; Glöckner et al. 2003; <strong>Jacobs</strong>-Wagner 2004).<br />
Glöckner et al. (Glöckner et al. 2003) published the first complete genome sequence of the<br />
marine planctomycete Rhodopirellula baltica SH1 T . With that, the “genomic revolution”<br />
reached the Planctomycetes. Several genome projects have been initiated in the last year in<br />
order to get the complete picture of the potential of this phylum. An early draft of the genome<br />
of the freshwater isolate of Gemmata obscuriglobus UQM 2246 T (Franzmann and Skerman<br />
1984) from the Institute for Genomic Research (TIGR) project is now available. Draft<br />
sequences of the two marine planctomycetes Blastopirellula marina DSM 3645 T (Schlesner<br />
et al. 2004) and Planctomyces maris DSM 8797 T (Bauld and Staley 1976) are funded by the<br />
Gordon and Betty Moore foundation on behalf of the Max Planck Institute, Bremen and are<br />
now almost completely annotated (Woebken et al. 2007). Finally, the anammox<br />
planctomycete Candidatus Kuenenia stuttgartiensis was investigated by a metagenomic<br />
project (Strous et al. 2006).<br />
3
Background<br />
1.2.1 Rhodopirellula baltica<br />
Pirellula sp. strain 1, which now has been validated as "Rhodopirellula baltica<br />
SH1 T "(Schlesner et al. 2004), is a marine aerobic, heterotrophic representative of the phylum<br />
Planctomycetes. R. baltica was isolated from the<br />
water column in the Kiel fjord which is part of the<br />
Baltic Sea. The whole genome sequence of<br />
R. baltica has been completed and published<br />
(Glöckner et al. 2003) by the REGX project research<br />
group at the Max Planck Institute for Marine<br />
Microbiology, Germany (www.regx.de). With 7,145<br />
Mb and 7,325 open reading frames, the genome of<br />
R. baltica represented one of the largest bacterial<br />
genomes sequenced at that time.<br />
Fig 1 Rhodopirellula baltica EM (Schlesner et al. 2004)<br />
1.2.1.1 Division and cell-cycle<br />
The pear-shaped adult cells of Rhodopirellula baltica are non-motile and display a polar<br />
organisation. From the smaller cell pole a holdfast substance of yet unknown chemical<br />
composition is excreted. It mediates attachment of cells to surfaces or to each other leading to<br />
the formation of rosettes (Fig 2). The broader cell pole is the site of budding. The bud is a<br />
mirror image of the mother cell, except that it is smaller in size and has a single flagellum<br />
inserted in a sub polar position at the proximate pole. At a certain developmental stage the<br />
bud (daughter cell) is released as a motile swarmer. The swarmer cells abort their flagellum,<br />
when differentiating into adult cells. Only these adult cells possess fimbriae that originate<br />
from so-called crateriform structures at the broader (reproductive) cell pole (Olga Lang,<br />
personal. communication). While these crateriform structures are restricted to the broader cell<br />
pole in adult cells, they are observed across the complete cell surface of buds (Gade et al.<br />
2005).<br />
Gade and coworkers (Gade et al. 2005) showed that there is a correlation between distinct<br />
morphotypes and different growth phases of R. baltica cultures based on microscopic<br />
examination and analysis. As illustrated in the growth curve in Fig 2, during early stages of<br />
growth numerous budding mother cells and swarmer cells are present. As growth proceeds,<br />
the number of swarmers decreases, and single cells begin to attach to each other forming<br />
4
Background<br />
rosettes. The latter are the dominating morphotype during transition to and in the stationary<br />
phase, where swarmer cells are rarely observed.<br />
Fig 2 Schematic drawing of the life-cycle of Rhodopirellula baltica. See text for details (Gade et al. 2005)<br />
The cellular mechanisms regulating these morphological changes during cell-cycle of<br />
R. baltica are not currently understood. For example during the unequal cell division nothing<br />
is known about how the cell compartments are distributed between the daughter cells. In<br />
general, the cell division machinery of Bacteria consists of different cell division genes, such<br />
as the filamentation temperature sensitive genes (fts) named ftsZ, ftsA, ftsI, ftsL, ftsQ, ftsN,<br />
ftsK, ftsW and the FtsZ interacting proteinA (zipA). The ftsZ-gene is the major constituent of<br />
the septal replication ring. Surprisingly, all of these genes, except ftsK, are absent from the<br />
genome of R. baltica. Interestingly, also not present in the genome of the planctomycete<br />
Gemmata obscuriglobus UQM2246 T . Teeling (Teeling 2004) concluded that the cell division<br />
in Planctomycetes, like in Chlamydia, and must go through a mechanism totally unknown at<br />
present.<br />
As noted earlier, morphological changes, during the life-cycle of R. baltica seem to be similar<br />
to C. crescentus CB15. Generally the key gene of the cell-cycle is the control protein CtrA. Its<br />
task as the essential master regulator is overseeing several important cellular processes<br />
(Stephens 2001). Within the R. baltica genome no homologue to the protein CtrA has been<br />
found. However some patterns (e.g. TTAAN 7 AAAC), were found which are similar to CtrA<br />
and CzcR – binding site patterns (TTAAN 7 TTAA).<br />
5
Background<br />
1.2.1.2 Substrate spectrum<br />
Physiological studies on R. baltica showed optimal growth between 28 and 30°C. The<br />
bacterium is strictly aerobic, glucose will not be fermented and nitrate cannot serve as an<br />
electron acceptor. Growth experiments on liquid yielded that R. baltica can utilise a wide<br />
range of mono- and disaccharides (Tab 1) (Schlesner et al. 2004).<br />
Tab 1 Substrates tested on Rhodopirellula baltica modified after Schlesner et al. (Schlesner et al. 2004)<br />
Substrates used as carbon source<br />
Substrates not used as carbon source<br />
Monosaccharides<br />
C6: glucose, fructose, mannose, galactose, trehalose,<br />
C5: lyxose, ribose, xylose<br />
Monosaccharides<br />
C6: sorbose<br />
C5: -<br />
Modified monosaccarides<br />
methylated: rhamnose<br />
N-acetylated: N-acetylglucosamine<br />
Others: esculin, salicin<br />
Disaccharides<br />
cellobiose, lactose, maltose, sucrose, melibiose,<br />
amygdalin<br />
Trisaccharides<br />
melezitoase, raffinose<br />
Polysaccarides<br />
chondroitin sulphate, gelatine, starch, dextrin<br />
C1<br />
-<br />
Others<br />
glycerol, gluconate, glucuronate<br />
Modified monosaccarides<br />
methylated: fucose<br />
N-acetylated: -<br />
Others: -<br />
Disaccharides<br />
-<br />
Trisaccharides<br />
-<br />
Polysaccarides<br />
cellulose, chitin, alginate<br />
C1<br />
methylamine, methylsulfonate, methanol<br />
Others<br />
methanol, ethanol, erythritol, adonitol, arabitol,<br />
dulcitol, inositol, mannitol, sorbitol, acetate,<br />
adipate, benzoate, caproate, citrate, formiate,<br />
fumarate, glutarate, lactate, malate, 2-<br />
oxoglutarate, phthalate, propionate, pyruvate,<br />
succinate, tartrate, norleucine, ornithine, urea,<br />
indole, inulin, pectin, casein, tween 80, all 20<br />
amino acids<br />
N-acetylglucosamine (NAG) can be used as the only energy and nitrogen source (Rabus et al.<br />
2002). NAG is a main structural component in chitin and a substantial constituent of the<br />
6
Background<br />
bacterial peptidoglycan cell wall. It is one of the main amino sugars in marine habitats<br />
(Riemann and Azam 2002). Additional growth studies show that R. baltica can grow on<br />
chondroitin sulphate (component of fish cartilage) (Schlesner et al. 2004) and iota-carrageenplates<br />
(algae) (Gurvan Michel pers. communication).<br />
The annotation process identified the standard pathways for heterotrophic bacteria including<br />
glycolysis, citrate cycle and oxidative phosphorylation. R. baltica SH1 T lacks the glyoxylate<br />
bypass and the Entner-Doudoroff pathway but exhibits the pentose phosphate cycle.<br />
Unexpected for an aerobic heterotrophic bacterium was the presence of all genes for<br />
heterolactic acid fermentation, though the organism has not yet been successfully cultured<br />
under anoxic conditions. Interestingly, key genes for the inter-conversion of C1-compounds<br />
were also found. Previously these were believed to occur only in methanogenic Archaea and<br />
methylotrophic Alphaproteobacteria (Glöckner et al. 2003; Bauer et al. 2004).<br />
1.2.1.3 Sulphatases and their role<br />
The annotation results showed that the R. baltica genome harbours 110 genes encoding<br />
proteins with significant similarity to prokaryotic (82 genes; 75%) and eukaryotic (28 genes;<br />
25%) sulphatases. For instance, similarities were found to alkylsulphatase of Pseudomonas<br />
aeruginosa, to arylsulphatases of Pseudomonas sp., to mucin-desulfating sulphatase of<br />
Prevotella sp., and to archaeal arylsulphatase, as well as to mammalian iduronate-2-<br />
sulphatase and arylsulphatases A and B.<br />
The annotation also revealed that in R. baltica the sulphatase genes are distributed across the<br />
genome in 22 clusters containing two to five genes. 59 sulphatases out of the set of 110<br />
predicted sulphatases contain signal peptides prediction, indicating that most of these proteins<br />
are probably secreted. 11 predicted sulphatases have a high expression level prediction (PHX)<br />
(Lombardot 2004).<br />
In comparison, analysis of 70 published prokaryotic genomes with a specific Pfam profile<br />
revealed a maximum of only 6 sulphatases found in the Pseudomonas aeruginosa PAO1<br />
genome and 30 in Bacteriodes thetaiotaomicron (Lombardot 2004). However, recent studies<br />
conclude that the number of genes and bacterial species encoding sulphatase enzymes is<br />
currently underestimated (Berteau et al. 2006). Until now, the 110 sulphatases found in<br />
R. baltica (15.3 per Mb) represents the highest number in any sequenced bacterial genome. In<br />
other planctomycetal genomes, genes encoding sulphatases were found in various numbers.<br />
Planctomyces maris DSM8797 T draft genome codes for 87 sulphatases (10.7/Mb), in<br />
Blastopirellula marina DSM3645 T 41 were found (6.16/Mb) and in the freshwater<br />
7
Background<br />
planctomycete Gemmata obscuriglobus UQM2246 T only 11 sulphatases are present<br />
(1.37 Mb) (Woebken et al. 2007). Another marine organism, Zobellia galactanivorans,<br />
belonging to the CFB group has around 70 genes annotated as sulphatases (Gurvan Michel,<br />
personal communication).<br />
Sulphatases are widespread enzymes found in Bacteria, Archaea and Eukarya. They are<br />
involved in various metabolic processes, ranging from a sulphate starvation response in<br />
bacteria to hormone biosynthesis and the modulation of developmental cell signalling in<br />
mammals (Parenti 1997). In humans, their biological relevance is particularly underlined by<br />
their involvement in several inherited diseases, such a mucopolysaccaridoses. It is known that<br />
sulphatases act on a broad diversity of substrates, which leads to their classification by the<br />
IUBMB into 17 classes (from EC 3.1.6.1 to EC 3.1.6.18) (Berteau et al. 2006). The role of the<br />
sulphatases in R. baltica is not yet clearly understood.<br />
Investigations of the 110 sulphatases in R. baltica based on the transcriptome and proteome<br />
level revealed that at least a fraction of them are expressed (Gade et al. 2005; Gade et al.<br />
2005; Würdemann 2006). Sulphatase activity studies performed at the <strong>University</strong> of Graz<br />
(Wallner et al. 2004), show a high enantioselective sec-alkyl sulphatase activity with retention<br />
of configuration when several substrates where tested on resting whole cells of R. baltica<br />
In summary, the data on the nature of substrates utilised as carbon sources by R. baltica and<br />
the high numbers of sulphatase genes found in the genome and the proven activity of at least<br />
some of them suggest that sulphatases are metabolically important in R. baltica and could<br />
play a role in the efficient degradation of sulphated glycopolymers. Such compounds (e.g.<br />
carrageen) are abundant in marine environments in the form of phytodetrital macroaggregates<br />
(“marine snow”), and Planctomycetes have been shown to be components of the microbial<br />
communities on such aggregates (Glöckner et al. 2003; Woebken et al. 2007).<br />
8
Background<br />
1.3 Further outcome of the genome annotation and its limitations<br />
The availability of different planctomycetal genomes has led to an enormous flood of new<br />
information. This has led to the popular phrase that the pre-genome era was the “dark age”<br />
(Dunham 2000). However, it is well known that, in any newly sequenced bacterial genome<br />
30-40% of the genes do not have an assigned function. This number is even higher for<br />
archaeal and eukaryotic genomes and for the relatively large genomes of bacteria with a<br />
complex life-style, such as Anabaena and Streptomyces (Galperin and Koonin 2004). It is also<br />
valid for R. baltica. In 2003, of the 7325 potential proteins in R. baltica only 3380 (46%) had<br />
a significant hit when compared with public databases. This means that over 54% of proteins<br />
in the genome remain uncharacterised and will be referred to as “hypothetical proteins” or<br />
with the affix “conserved” in case of wider phyletic distribution (Galperin and Koonin 2004).<br />
So far, their function can only be subject to speculation. Some of these hypothetical proteins<br />
are planctomycete-specific or even unique to R. baltica. The former means that they only<br />
occur in a maximum of all four planctomycetal genomes. It seems likely that some of these<br />
genes code for the unique cellular planctomycetal characteristics and for planctomycetespecific<br />
metabolic traits. A large proportion of these planctomycete-specific genes carry one<br />
or more specific domains of as yet unknown function (DUF) (Woebken et al. 2007). Often<br />
they appear in groups and/ or before operon-like arrangements, indicating a potential<br />
regulatory function. This may be supported by the fact, that an unusually low number (2.4%)<br />
of transcriptional regulators from known families were found in the genome (Gade et al.<br />
2005).<br />
9
Background<br />
1.4 Gene expression profiling by DNA microarray analysis<br />
The genome annotation of R. baltica combined with the experimental work that has been<br />
carried out on this organism and on other members of the Planctomycetes provided new<br />
information and generated hypotheses about the metabolic capabilities and life-style of these<br />
organisms. However, in order to turn these mostly computationally derived hypotheses into<br />
real biological knowledge there is an urgent need to verify the predicted gene functions by<br />
experiments using different molecular techniques, for example gene expression profiling.<br />
Gene expression is a highly complex and tightly regulated process that allows a cell to<br />
respond dynamically both to environmental stimuli and to its own changing needs. This<br />
mechanism acts as both an “on/off” switch to control which genes are expressed in a cell, and<br />
as a ”volume control” that increases or decreases the level of expression of particular genes as<br />
necessary (Dupont et al. 2007). Gene expression analysis examines the composition of<br />
cellular messenger RNA populations.<br />
Traditional gene expression analysis has used techniques such as Northern blotting, RT-PCR<br />
and nuclease protection assays. More advanced methods – some of these include differential<br />
display, subtractive hybridisation, representational difference analysis, expressed sequence<br />
tags, cDNA fragment fingerprinting, and serial analysis of gene expression – have enabled the<br />
discovery of novel differentially expressed genes. However, the technical challenges of these<br />
methods still limit their use to the analysis of just a few samples/genes at a time. Microarray<br />
analysis, in contrast, allows the analysis of thousands of genes in multiple samples with<br />
relative ease (Duggan et al. 1999).<br />
Gene expression analysis using DNA microarrays has been applied to numerous mammalian<br />
tissues, yeast (Alizadeh and Staudt 2000), and bacteria alike (Hu et al. 2005; Steglich et al.<br />
2006). These studies examined the effects of different chemicals on cells, the consequences of<br />
over-expression of regulatory factors in transfected cells, and compared mutant strains with<br />
parental strains to delineate functional pathways. In cancer research, microarrays have been<br />
used to find gene expression changes in transformed cells and metastases, to identify<br />
diagnostic markers, and to classify tumours based on their gene expression profile (Amersham<br />
2002). Lately, transcriptional profiling is even applied to study environmental questions<br />
(Zhou 2003; Parro et al. 2007). Dupont and co-workers expect this to be the major activity of<br />
ecological genomics in the near future (Dupont et al. 2007).<br />
10
Background<br />
1.5 Principle of DNA microarray analysis<br />
DNA microarrays contain thousands of DNA samples or oligonucleotide sequences named<br />
probes (Fig 3). The probes are printed or synthesised in a precise and known pattern onto<br />
nylon membrane filter or microscope glass slide. In the latter case the slides are coated with<br />
chemically reactive groups typically aldehydes or primary amines to help to stabilise the DNA<br />
(probes) onto the slide, either by covalent bonds or electrostatic<br />
interactions. Applied onto the printed chip surface will be the labelled<br />
hybridisation partner, which is complementary to the probe and referred<br />
to as target (Fig 3). The basic principle of complementary base pairing<br />
underlies the specific hybridisation of these two molecule types, and is<br />
common to all microarray approaches.<br />
Fig 3 Nomenclature: tethered nucleic acid as “probe” (spotted oligos) and free<br />
nucleic acid as “target” (labelled cDNA) (Phimister 1999)<br />
A typical workflow of a cDNA microarray experiment used in gene expression analysis is<br />
summarised in Fig 4. First, the probes have to be designed by the use of a bioinformatic<br />
pipeline. This includes screening oligos for their thermodynamic properties as well as<br />
secondary structures, calculation of oligo Tm for uniform Tm value and BLAST searches<br />
against the organism to guaranty specificity. Meanwhile, immobilisation parameters like<br />
humidity and temperature used for slide production have to be optimised according to the<br />
chemical features of the printing tip and the slide surface chemistry.<br />
Once the probes have been synthesised and printed on the chip, focus is shifted to the sample.<br />
Our flowchart experiment (Fig. 4) compares the relative expression level of specific<br />
transcripts in two samples. One of these samples is the control, named reference (A) and the<br />
other is derived from cells whose response or status is being investigated (B). The sampling is<br />
followed by RNA extraction. Both steps are important components of the target preparation<br />
process since successful microarray studies depend on the consistent extraction of high<br />
quality RNA. The extracted sample and reference RNA are reverse-transcribed into cDNA<br />
and labelled with one of two fluorochromes: one fluorochrome for the reference and one<br />
fluorochrome for the sample (e.g. Cyanine- or Alexa- dyes). Different techniques of labelling<br />
are used. The label can be either introduced by direct enzymatic incorporation of fluorescently<br />
labelled nucleotides or by the direct labelling approach (Zhang et al. 2001; Gupta and<br />
Cherkassky 2003) and also indirectly by incorporation of aminoally-dUTP and subsequent<br />
11
Background<br />
coupling of fluorescent dyes (Beier et al. 2004). Equal amounts of the labelled target cDNA<br />
(reference + sample) are combined, co-hybridised under stringent conditions onto a<br />
microarray replicate and washed in a defined procedure. Afterwards the slide is scanned using<br />
a specialised laser scanner to record the emission of the two fluorochromes for each spot on<br />
the microarray after excitation at a given wavelength. The intensity of the fluorescence<br />
emission signals on each spot is proportional to transcript levels in the biological samples and<br />
the fluorescent signal from each gene probe is quantified by using specialised image<br />
processing software. The output can then be analysed and normalised using different analysis<br />
software. A standard method to evaluate the results is quantitative Real-Time (RT)-PCR.<br />
12
Background<br />
Fig 4 Flowchart of a typical microarray experiment modified after (Leung and Cavalieri 2003)<br />
13
Background<br />
1.6 Microarray data analysis<br />
Data analysis is an essential process in DNA microarray experiments, since these experiments<br />
normally result in a large amount of information. These data must be adequately processed to<br />
find statistically significant correlations – e.g. co regulation of genes - within and between<br />
different arrays (FIG 4).<br />
First, the hybridisation signal intensities must be filtered and normalised. This transformation<br />
is done to minimise the bias arising from unequal quantities of starting RNA, differences in<br />
labelling or detection efficiencies of the fluorescent dyes applied, and other systematic biases<br />
(Quackenbush 2002). For an overview on different normalisation methods see Foster and<br />
Ghazal 2003(Foster and Ghazal 2003). In the next step, data mining techniques are required to<br />
answer the biological question behind the experiment. Normally, microarray experiments are<br />
conducted to identify genes which are either under- or over-expressed after a shift in the<br />
experimental conditions. For example, we might be interested in genes that have an elevated<br />
expression because of a drug treatment. Such genes are most easily found by simple filtering.<br />
If the log-transformed data (method) is used for filtering, differentially expressed genes are<br />
inferred by a fixed threshold cut off method (i.e. a two-fold increase or decrease). Filtering by<br />
absolute expression change can even be used for experiments, where there are no replicates.<br />
However, there are also ranking-methods available [t-test (Pan 2002), ANOVA (Kerr et al.<br />
2000), Bayesian method or Mann-Whitney test]. All these methods produce errors (falsepositive<br />
and false-negatives), therefore differential gene expression is usually confirmed by<br />
RT-PCR or northern blots (Leung and Cavalieri 2003). In case of interest for co-regulation of<br />
genes (or related arrays), various cluster techniques should be considered. The basic concept<br />
in clustering is to try to identify and group together similarly expressed genes and to correlate<br />
the observations to biology. The idea is that co-regulated and functionally related genes are<br />
grouped into clusters. Some often used grouping techniques are hierarchical clustering (Eisen<br />
et al. 1998), k-means clustering (Soukas et al. 2000), self-organising maps (SOMs) (Kohonen<br />
1992) and principal component analysis (PCA) (Raychaudhuri 2000) (Methods reviews<br />
Quackenbush 2002; Gollub and Sherlock 2006). There is no clustering method that can be<br />
applied for all kinds of experiments. Different cluster methods used on the same data set can<br />
reveal unique aspects of the data (Leung and Cavalieri 2003). It is therefore advisable to<br />
analyse the data using several methods rather than just one (Leung 2002).<br />
14
Background<br />
Finally, cluster data can be mined for e.g. promoter elements involved in the regulation<br />
observed (Brazma and Vilo 2000), for novel functions of orphan proteins, and for novel<br />
regulatory relationships between genes under study (Knudsen 2004). Then, gene expression<br />
patterns can be correlated with physiology through the functional networks (pathways) and<br />
regulatory mechanisms in which the relevant genes are involved. A wide variety of resources<br />
and tools exists to display pathway information, enabling presentation of microarray data in<br />
the framework of documented biological pathways (Imbeaud and Auffray 2005).<br />
15
Background<br />
1.7 Microarray data management<br />
Data warehousing for microarray experiments is an informatic challenge due to the thousands<br />
of elements a typical microarray experiment will produce. There are two types of storage<br />
systems, local for in-house data management and public array databases for meta-mining.<br />
Raw data in the form of images carry the most information but are difficult to share<br />
effectively through the internet due to their large size. Analysed data, however, can be<br />
disclosed easily but a lot of information essential for quality assessment is lost during the<br />
analysis process. Attempts to compare data from different databases without realising their<br />
underlying incomparability are devastating to the future development of the microarray field.<br />
Consequently, there is an urgent need to set up a standardised definition for experimental<br />
information to guide the development of a comparable and integrated experimental design and<br />
database system (www.mged.org/Workgroups/MIAMI/miami.html) (Leung 2002). A solution<br />
has been presented in the Minimum Information About a Microarray Experiment (MIAMI)<br />
document offered by the Microarray Gene Expression Data Society (MGED) working group.<br />
The document describes the minimum information required to ensure that microarray data can<br />
be easily interpreted and that results derived from its analysis can be independently verified<br />
(Brazma et al. 2001).<br />
The aphorism “Well begun is half done” is especially true for microarray analysis since<br />
adequate experimental design is extremely important for these kinds of investigations. Leung<br />
and Cavalieri (Leung and Cavalieri 2003) summarise in their review what a good microarray<br />
experimental design should comprise. (i) A clearly defined biological question and/or<br />
hypothesis; (ii) treatment, perturbation and observation of the biological materials, as well as<br />
the microarray experimental protocols should be as little affected by systematic and<br />
experimental errors as possible; (iii) a simple, sensible and sound microarray experimental<br />
arrangement that will yield the maximal amount of information given in the cost structure and<br />
complexity of the study (review by (Churchill 2002)) and (iv) compliance with the MIAMI<br />
(Leung and Cavalieri 2003).<br />
16
Research Aims<br />
2 RESEARCH AIMS<br />
2.1 The Rhodopirellula baltica life-cycle: Growth-dependent<br />
expression profiling of the complete genome with a cDNA-based<br />
microarray<br />
As in any other bacterium, regulatory networks must coordinate cell-cycle progression with<br />
cell growth, including temporal and spatial control of DNA replication and cytokinesis, to<br />
ensure equal partitioning of chromosomes into daughter cells. The completion and annotation<br />
of the R. baltica genome sequence has allowed a first estimate of the genetic potential.<br />
However, nothing is known so far about the genetic network controlling development, the<br />
cell-cycle and the cellular mechanisms regulating these morphological changes in R. baltica.<br />
It is anticipated that the genes functioning in these networks should show varying mRNA<br />
levels over the life-cycle. Therefore growth-dependent expression patterns could give hints<br />
about genes that might be required in physiological processes, morphotypes and cellular<br />
activity typical for R. baltica. The approach of microarray technology is particularly useful<br />
when applied to complex processes such as the cell-cycle, where many genes are expected to<br />
be involved (Spellman et al. 1998; Laub et al. 2000). Global assays of gene expression during<br />
the Caulobacter crescentus cell-cycle revealed that surprisingly 19% of the genome is<br />
affected as cells grow and divide (Jenal and Stephens 2002).<br />
The whole genome array will be a means to explore the regulation of all genes during the lifecycle.<br />
Clustering of all genes could help to discriminate the function of potential hypothetical<br />
proteins through co-regulation with proteins of known function and also to assign a function<br />
to clustered hypothetical proteins because of their specific cell-cycle pattern. Furthermore,<br />
analysing the set of cell-cycle regulated genes for potential promoter elements could give<br />
some hint about unknown regulatory mechanisms for genes in R. baltica. Fortunately, cellcycle<br />
proteome data are also available for comparison with obtained transcriptome analysis<br />
data (Gade et al. 2005).<br />
At the same time, fluorescence imaging of membrane stained (Lindsay et al. 2001) dividing<br />
R. baltica cells could help to understand how the division process of the compartments in the<br />
three-dimensional space is taking place. Combining the “omic” knowledge and with the<br />
fluorescence imaging results contribute to our understanding of the underlying molecular cell<br />
biology of the cell compartmentalisation of the phylum Planctomycetales.<br />
17
Research Aims<br />
2.2 Expression profiling of the sulphatases genes of the<br />
planctomycete Rhodopirellula baltica grown on different sulphated<br />
polysaccharides<br />
The carbon cycle in natural environments depends on the remineralisation of biomass. In<br />
marine systems biomass is mainly produced by phototrophic microorganisms in the upper<br />
layer of the water column (annual production of 20 − 30 × 10 9 tons of carbon). Dead biomass<br />
settles to the sediment, often aggregating as particles known as marine snow. During this<br />
process, organic carbon is already decomposed by aerobic microorganisms (Rullkötter 1999).<br />
Since polysaccharides are major components of biomass, carbohydrate degradation is<br />
particularly relevant for carbon turnover. R. baltica and other members of the Planctomycetes<br />
are considered key players in carbohydrate metabolism in marine systems, because of their<br />
nutritional specialisation, their high abundance in the marine water column and their<br />
association with marine snow, and also the high number of sulphatases genes found in their<br />
genome.<br />
The role of the sulphatases however, is still unclear. The presence of 110 sulphatase genes of<br />
unknown physiological role in the R. baltica genome represents an ideal starting point for<br />
further functional analysis. Expression profiling of the 110 sulphatase genes and enzymes<br />
from associated pathways (ECF-sigma factor, sugar degradation) in R. baltica provides a<br />
deeper view of the regulation and expression of bacterial sulphatases and their role in the<br />
natural habitat of the organism. First expression profiling experiments on a partial genome<br />
array were carried out with chemically labelled cDNA extracted from R. baltica cultures<br />
grown on glucose and N-acetylglucosamine, ribose and chrondroitinsulphate A and C.<br />
Although some differences in the expression patterns could be detected it could also be seen<br />
that most of the sulphatases under investigation were already expressed during cultivation<br />
with glucose (reference) (Würdemann 2006). The reason for their obvious constitutive<br />
expression is unclear. Further microarray experiments with R. baltica cultures grown on<br />
different oligosaccharides allow specification of the substrate preference of different<br />
sulphatases. The different polysaccharides derived from different marine macroalgae are<br />
provided by cooperation with the CNRS – Laboratoires GOËMAR – UPMC of Prof.<br />
B. Kloareg in Roscoff: iota- and kappa carrageenan (Rhodophyta) (Barbeyron et al. 2000),<br />
alginate and fucane (Phaeophyta) (Descamps et al. 2005).<br />
Bioinformatic analysis based on the similarity comparison of the sulphatase genes found in all<br />
available Planctomycete genomes and in the genome of the marine bacteroidetes<br />
18
Research Aims<br />
Zobellia galactanivorans are carried out to identify the number of sulphatase genes<br />
responsible for the degradation of polysaccharides.<br />
If expression or regulation of some sulphatases under special conditions is detectable, an in<br />
depth characterisation of the catalysed reaction would be interesting also for industrial use,<br />
e.g. for the food industry where sulphatases are needed that selectively remove part of the<br />
sulphate groups, thereby stabilising the consistency of the chemical network. Such approaches<br />
are used to thicken cream, aerate mousse and suspend the herbs in salad dressing<br />
(www.degussa.com).<br />
19
Research Aims<br />
2.3 Design of the whole genome array of Rhodopirellula baltica<br />
In 1999, Lander called DNA microarrays 'arrays of hope' because for the first time it was<br />
possible to take “global views” of biological processes at the genome level (Lander 1999).<br />
This statement nicely matches the expectations linked to the whole genome array of the<br />
marine planctomycete Rhodopirellula baltica. The design of the array was driven by the<br />
ambition to understand more about the role of R. baltica, representing the phylum<br />
Planctomycetes as one of the key players in the carbon cycle and about its adaptation<br />
mechanisms to changing environmental conditions and cell biology. Microarray-mediated<br />
expression profiling should also to help reveal regulation-patterns of the large number of<br />
genes encoding hypothetical proteins.<br />
A conventional whole genome array targeting all 7325 genes annotated within the R. baltica<br />
genome (Glöckner et al. 2003) is set up within the framework of the Network of Excellence<br />
Marine Genomics Europe (MGE). So far, no universal microarray hybridisation protocol was<br />
available (Li et al. 2002). Therefore, every slide type or chemical surface, every new target<br />
preparation process and labelling method, hybridisation and washing buffer composition as<br />
well as hybridisation time and conditions have to be optimised corresponding to the new<br />
application. Moreover, the specific proteinaceous cell wall of R. baltica makes it difficult to<br />
apply common RNA extraction, cDNA-transcription and labelling methods, and thus requires<br />
substantial methodological adaptations.<br />
In parallel, a pipeline for microarray data processing and data storage has to be implemented.<br />
Different microarray data analysis software tools are reviewed with the focus on usability and<br />
calculation transparency. Moreover, a database for storing the data in-house needs to be<br />
initiated. The validation/quality of the resulting data during the optimisation process needs to<br />
be checked by the spotted positive and negative controls and consistency with published<br />
results of proteome studies (Gade et al. 2003; Gade et al. 2005).<br />
20
Results and Discussion<br />
3 RESULTS AND DISCUSSION<br />
Manuscripts and Publications<br />
3.1 Transcriptional response of the marine planctomycete Rhodopirellula baltica SH1 T to<br />
changing environmental conditions<br />
Manuscript submitted to BMC Genomics<br />
Authors: Patricia Wecker, Christine Klockow, Christian Quast, Andreas Ellrott, Phillip<br />
Langhammer, Jens Harder and Frank Oliver Glöckner<br />
Contribution: Idea and concept, development of the manuscript under editorial cooperation of<br />
F.O.G<br />
3.2 The Rhodopirellula baltica life-cycle: Growth-dependent expression profiling of the<br />
complete genome with microarrays<br />
Manuscript in preparation<br />
Authors: Patricia Wecker, Marga Schüler, Andreas Ellrott, and Frank Oliver Glöckner<br />
Contribution: Idea and concept, development of the manuscript under editorial cooperation of<br />
M.S. and F.O.G<br />
21
Results and Discussion<br />
3.3 Highly enantioselective sec-alkyl sulfatase activity of the marine planctomycete<br />
Rhodopirellula baltica shows retention of configuration<br />
Published in: Angewandte Chemie International Edition 44: 2 –4 (2005)<br />
Authors: Sabine R. Wallner, Marga Bauer, Chris Würdemann, Patricia Wecker, Frank Oliver<br />
Glöckner, and Kurt Faber<br />
Contribution: Selection of the media composition, Cell culturing and growth, Cell harvesting<br />
and storage<br />
3.4 Fosmids of novel marine Planctomycetes from the Namibian and Oregon coast upwelling<br />
systems and their cross-comparison with planctomycete genomes<br />
Published in: The ISME Journal 1, 419-435 (2007)<br />
Authors: Dagmar Woebken, Hanno Teeling, Patricia Wecker, Alexandra Dumitriu, Ivaylo<br />
Kostadinov, Rudolf Amann, Edward F DeLong and Frank Oliver Glöckner<br />
Contribution: Microarray experiments and Data analysis<br />
22
Results and Discussion<br />
3.1 Transcriptional response of the model planctomycete<br />
Rhodopirellula baltica SH1 T to changing environmental conditions<br />
Authors: Patricia Wecker, Christine Klockow, Christian Quast, Andreas Ellrott, Phillip<br />
Langhammer, Jens Harder, and Frank Oliver Glöckner<br />
Manuscript submitted to BMC Genomics<br />
23
Results and Discussion<br />
Transcriptional response of the model planctomycete<br />
Rhodopirellula baltica SH1 T to changing environmental<br />
conditions<br />
Patricia Wecker 1,3 , Christine Klockow 1,3 , Andreas Ellrott 1 , Christian Quast 1 , Philipp<br />
Langhammer 2 , Jens Harder 2 , Frank Oliver Glöckner 1,3§<br />
1 Microbial Genomics Group, Max Planck Institute for Marine Microbiology, Microbial<br />
Genomics Group, Celsiusstr. 1, 28359 Bremen, Germany<br />
2 Department of Microbiology, Max Planck Institute for Marine Microbiology, Microbiology,<br />
Celsiusstr. 1, 28359 Bremen, Germany<br />
3 <strong>Jacobs</strong> <strong>University</strong> Bremen gGmbH, Campusring 1, 28759 Bremen, Germany<br />
§ Corresponding author<br />
Keywords: Planctomycetes, Rhodopirellula baltica, marine, expression profiling,<br />
hypothetical genes, shift experiments, whole genome array, microarray, heat shock, cold<br />
shock, high salinity<br />
Email addresses:<br />
P. Wecker: pwecker@mpi-bremen.de<br />
C. Klockow: cklockow@mpi-bremen.de<br />
A. Ellrott: aellrott@mpi-bremen.de<br />
C. Quast: cquast@mpi-bremen.de<br />
P. Langhammer: phillanghammer83@yahoo.de<br />
J. Harder: jharder@mpi-bremen.de<br />
F.O. Glöckner: fog@mpi-bremen.de<br />
24
Results and Discussion<br />
Abstract<br />
Background: The marine model organism Rhodopirellula baltica SH1 T was the first<br />
Planctomycete to be completely sequenced. The genome analysis predicted a complex life<br />
style and a variety of genetic opportunities to adapt to the marine environment. Its adaptation<br />
to environmental stressors was studied by transcriptional profiling using a whole genome<br />
microarray.<br />
Results: Stress responses to salinity and temperature shifts were monitored in time series<br />
experiments. Chemostat cultures grown on mineral medium at 28°C were compared to<br />
cultures that were shifted to either elevated (37°C) or reduced (6°C) temperatures as well as<br />
high salinity (59.5‰) and observed over 300 min. Heat shock showed the induction of<br />
several known chaperone genes. Cold shock altered the expression of genes in lipid<br />
metabolism and stress proteins. High salinity resulted in the modulation of genes coding for<br />
compatible solutes, ion transporter and morphology. In summary, over 3000 of the 7325<br />
genes were affected by temperature and/or salinity changes.<br />
Conclusions: Transcriptional profiling confirmed that R. baltica is highly responsive to<br />
its environment. The distinct responses gave new insights into the complex adaptation<br />
machinery of an environmentally relevant marine bacterium. Our transcriptome study and<br />
previous proteome data suggest a set of genes of unknown functions that are most probably<br />
involved in the global stress response. This work lays the foundation for further bioinformatic<br />
and genetic studies which leads to a comprehensive understanding of the biology of a marine<br />
Planctomycete.<br />
25
Results and Discussion<br />
Background<br />
Marine ecosystems, covering approximately 71% of the Earth’s surface, host the majority of<br />
biomass and contribute significantly to global cycles of matters and energy. Microorganisms<br />
are known to be the ’gatekeepers‘ of these processes, and any insights into their lifestyle and<br />
fitness enhances our ability to monitor, model and predict the effect of global changes.<br />
Nevertheless, the specific knowledge about their functions is still sparse. The ‘genomic<br />
revolution’ [1] has opened the door to investigate their genetic potential and activity on the<br />
molecular level.<br />
A particularly interesting representative of the marine picoplankton community is<br />
Rhodopirellula baltica, a free-living bacterium which was isolated from the water column of<br />
the Kiel Fjord (Baltic Sea) [2]. R. baltica belongs to the phylum Planctomycetes, a broadly<br />
distributed group of bacteria, whose members can be found in terrestrial, marine and<br />
freshwater habitats [3-7], but also in extreme environments like hot springs [8], marine<br />
sponges [9] and the hepatopancreas of crustaceans [10].<br />
In terms of cell biology all Planctomycetes share several morphologically unique properties,<br />
such as a peptidoglycan-lacking proteinaceous cell wall [11, 12], intracellular<br />
compartmentalization [13] and a mode of reproduction via budding. The latter results in a<br />
cell cycle that is characterized by motile and sessile morphotypes similar to Caulobacter<br />
crescentus [14-17]. A specific holdfast substance produced by sessile cells allows R. baltica<br />
to attach to macroscopic detrital aggregates (marine snow) [3, 18].<br />
At present, four planctomycete genomes are currently available [19], of these the genome of<br />
R. baltica is the only one completely closed [16]. The genome was found to be 7,145,576<br />
bases in size and codes for 7325 open reading frames (ORFs) plus 72 RNA genes. Originally<br />
for only 45% of the ORFs particular functions could be assigned. This means that over 55%<br />
of all proteins in the genome remain functionally uncharacterized. They were referred to as<br />
’hypothetical proteins‘ or with the affix ’conserved‘ in case of a wider phylogenetic<br />
distribution [20]. A subset of these conserved hypothetical proteins is specific for<br />
Planctomycetes [19]. It seems likely that some of these genes code for the unique<br />
planctomycetal cellular characteristics and metabolic traits.<br />
The availability of the genome information triggered several key post-genomic studies<br />
including studies of the proteome [21-26] enzyme activity [27] and protein crystallization<br />
[28].<br />
26
Results and Discussion<br />
In summary, these studies confirmed the hypothesis of Glöckner et al. [16] that R. baltica is a<br />
polysaccharide degrader. It seems that R. baltica is gaining carbon and energy from the<br />
decomposition of complex heteropolysaccharides originally produced by algae in the photic<br />
zone while slowly sedimenting with the marine snow.<br />
Marine microorganisms like R. baltica are exposed to rapidly changing environmental<br />
conditions, e.g. varying temperature, salinity, irradiance and oxygen concentration. Typically,<br />
sudden changes of these environmental conditions induce a stress response in the exposed<br />
planktonic community characterized by a distinct change in their gene expression pattern.<br />
This stress response enables the organisms to protect vital processes and to adapt to the new<br />
condition, which has been shown for a set of organisms in different environments, e.g. for<br />
Shewanella oneidensis [29, 30], Pseudomonas aeruginosa [31],<br />
Desulfovibrio vulgaris Hildenborough [32], Xylella fastidiosa [33], Synechocystis sp. [34]<br />
and Yeast [35].<br />
To gain insights into the stress responses of R. baltica with respect to salinity and<br />
temperature the first whole genome array for R. baltica, which is also the first planctomycete<br />
microarray, was established and applied. The reported data will serve as a resource to expand<br />
our understanding of the physiological and transcriptional response of R. baltica to the wide<br />
range of changing environmental conditions, a free-living marine bacterium is exposed to.<br />
Results and Discussion<br />
Overview<br />
54 different total RNA samples were analyzed by whole-genome microarray hybridization. In<br />
summary, for heat shock 2372 genes, for cold shock 922 genes and for salt stress 1127 genes<br />
exhibited significant differential expression at one or more of the five time points compared<br />
to the reference samples (FIGURE 1 i; ii & iii). With only 45% of functionally annotated<br />
genes in the genome it is not surprising that most of the differentially expressed genes were<br />
hypothetical or conserved hypothetical proteins. The complete list of the differentially<br />
expressed genes for each shift experiment and time point is available in the ADDITIONAL<br />
FILE 1.<br />
A COG function could be assigned to only 32% of the regulated genes in the heat and cold<br />
shock experiments (FIGURE 2 i & ii) compared to 37% in the salt stress (FIGURE 2 iii).<br />
This is in line with the 36% (2661 genes) COG functional class designations in R. baltica. A<br />
27
Results and Discussion<br />
striking feature of the expression profiles displayed is that a large fraction of the genome<br />
responded in a stereotypical manner to all three stressful conditions tested. In summary 152<br />
genes are up- or down-regulated at any time point for all stressors. Out of the 152 genes 62<br />
are induced and 90 are repressed (TABLE 2 & TABLE 3). 49% of the induced and 61% of<br />
the repressed genes were annotated as hypothetical proteins. The Venn diagrams shown in<br />
FIGURE 3 provide an overview of the specific and common genes of the three stress-specific<br />
responses. To identify co-regulated patterns of gene expression, we classified all<br />
differentially expressed genes of all three stress expressions into 30 k-means clusters based<br />
on their expression log ratio. The cluster data are available in the ADDITIONAL FILE 2.<br />
Cluster 1, 3 and 4 show a similar response to the specific environmental changes, called<br />
environmental stress response (ERS) over all experiments. Cluster 2, 4, 5, 7, 15 and 22<br />
revealed genes that are reacting only to one or inverse to a specific environmental factor.<br />
Experimental design and assessment of array data quality<br />
The experimental conditions were chosen to mimic the natural environment of R. baltica with<br />
the constraint that the stress response must be detectable by the microarray technology and<br />
should therefore be significantly pronounced. In contrast to steady-state or single-time-point<br />
studies, time series experiments can provide useful data for generating computational models<br />
of stress response pathways [29].<br />
The negative, positive and stringency controls printed on the array gave no indications for<br />
unspecific hybridizations. Co-hybridizations of two cDNA samples prepared from the same<br />
total cellular RNA (self-self hybridization) suggested that genes with an expression log ratio<br />
value greater than 1.5 and smaller than -1.0 for heat and cold shock, respectively, could be<br />
regarded as differentially expressed. For the salt stress log ratio values over 1.2 and<br />
below -1.0 were considered as significant.<br />
Effect of stress on Rhodopirellula baltica<br />
No growth was detectable during stress conditions nor was any obvious morphology change<br />
visible under the microscope. Under optimal conditions R. baltica has a doubling time of 10-<br />
12 hours [22], indicating that physiological effects are not measurable during the short time<br />
period of max. 5 hours the cells were exposed to stress.<br />
28
Results and Discussion<br />
Specific results of the shift experiments<br />
Heat shock<br />
In their natural environment R. baltica cells can be regularly exposed to higher temperatures<br />
e.g. at the water surface. Therefore, R. baltica cells were rapidly shifted from 28°C to 37°C<br />
and observed over a period of 300 min in the first experiment. This is approximately 9°C<br />
above the optimal growth temperature reported by Schlesner et al. [2]. A higher temperature<br />
would have killed the cells. The time series reveals a quick response of R. baltica to sudden<br />
temperature up-shifts. In total 2372 genes are regulated out of which 1140 genes encode<br />
hypothetical proteins. 390 genes (5%) were already regulated after 10 min. This number<br />
increased to 750 genes (10%) after 300 min (FIGURE 1 i). The COG classes containing the<br />
translation [J] and amino acid transport and metabolism [E] were the largest classes downregulated.<br />
Up-regulated genes were assigned to the COG classes replication, recombination<br />
and repair [L], post-translation modification, protein turnover and chaperons [O],<br />
transcription [K], secondary metabolites biosynthesis, transport and catabolism [Q], cell<br />
envelope biogenesis, outer membrane [M] and general function prediction [R] (FIGURE 2 i).<br />
Taking a closer look at the response of R. baltica to thermal stress revealed the induction of<br />
many known heat shock proteins (Hsp): ClpB (RB6751), GroEL (RB8970), DnaJ (RB8972),<br />
GrpE (RB8974), Hsp20 (RB10279, RB10283), dnaK (RB9105), as well as the ATPdependent<br />
protease ClpP (RB9103). Also up-regulated are the chaperonins Cpn10 (RB10627<br />
and RB8969) and Cpn60 (RB8966) as well as the cell division protein FtsH (RB2966)<br />
(Cluster 4 in Additional files 2). Previous proteomic studies found the proteins of these genes<br />
as well, except FtsH, DnaJ and Hsp20 [25, 26].<br />
The regulation of the heat shock response in R. baltica involves many transcriptional<br />
regulators. TetR (RB838) and GntR (RB1862, RB8695) showed an up-regulation, which<br />
confirmed their important role in early heat shock response [36]. A gene encoding for GntR<br />
was also found in the environment on the planctomycete fosmid 3FN from the metagenome<br />
study of the Namibian coast [19]. In E. coli the induction of the majority of heat shock genes<br />
results from a rapid and transient increase in the cellular level of an alternative 32-kDa sigma<br />
factor (sigma32) encoded by rpoH and by alternative sigma factors E and 54, encoded by<br />
rpoE (RB2302) and rpoN (RB6491), respectively [29]. Although, all genes are present in the<br />
R. baltica genome, they were not regulated, suggesting a significantly different response<br />
cascade.<br />
29
Results and Discussion<br />
R. baltica also showed an extracyctoplasmic stress response. The gene coding for SecA<br />
(RB11690), belonging to the Sec system, was induced. This indicated an activation of the<br />
protein translocation most probably from the riboplasma to the paryphoplasm or maybe also<br />
to the medium. Proton channels were induced and R. baltica inhibited the motility. The<br />
flagellar motor switch protein (FliG - RB12502) was already down-regulated after 20 min.<br />
Followed by the type 4 fimbrial assembly protein (pilC - RB11597) after 40 min.<br />
Cold Shock<br />
During sedimentation, e.g. attached to marine snow flocks, R. baltica needs to cope with<br />
decreasing temperatures. To investigate the response to cold shock, R. baltica cells were<br />
shifted from 28°C to 6°C and observed for a period of 300 min. 6°C was chosen for this<br />
study because this is a common temperature in the Baltic Sea. Also, the temperature<br />
difference of 22°C is generally regarded as standard for cold shock studies with bacteria [29,<br />
37]. Compared to heat shock only one third (922) of the regulated genes were differentially<br />
expressed. Out of these 922 regulated proteins, 391 genes (42%) encode for hypothetical<br />
proteins. With 419 differentially expressed genes (6%) the cold shock response reached its<br />
peak after 20 min and was decreasing afterwards (FIGURE 1 ii). In contrary to the heat shock<br />
experiment it seemed that R. baltica needed approximately one hour to adapt to cold<br />
conditions. Like other bacteria R. baltica responded to cold conditions with the up-regulation<br />
of genes coding for stress response [COG class O], cell envelope and transport [M],<br />
transcription factors and solute uptake. Genes for amino acid biosynthesis [E], protein fate<br />
and synthesis [J] were down-regulated (FIGURE 2 ii) [30].<br />
The transcriptional activity was regulated by the up-regulation of diverse RNA polymerase<br />
sigma factors, like rpoD (RB6780) and sigK (RB1392). A homolog of rpoD (RB6780) was<br />
also found on the planctomycete fosmid 13FN [19]. 20 min after the exposure of R. baltica to<br />
cold conditions it started to express genes that change the cytoplasmic membrane<br />
composition and fluidity as well as its morphology. The alteration of the lipid composition in<br />
the cold has been previously reported from other microorganisms [38]. In R. baltica genes<br />
coding for cell envelope (RB6114 and RB6895), transport (RB4870), lipid metabolism<br />
(RB316) and 18 genes coding for membrane proteins were repressed after 20 min.<br />
Furthermore, R. baltica repressed genes involved in sporulation oppB (RB12861) and O-<br />
antigen flippase (RB2503), flaA (RB4454) and pilus assembly (RB4061 and RB5478),<br />
leading to a reduced motility and budding ability. Genes associated to the amino acid<br />
30
Results and Discussion<br />
biosynthesis, especially to the synthesis and fate of glutamine (RB4269) and glutamate<br />
(RB5653) were also affected. Latter are shown to be translated [25, 26]. A<br />
glycosyltransferase (RB12831) and glycosidases (RB2988, RB2990 and RB2991) are upregulated<br />
at 300 min probably for remodeling of the cell wall.<br />
Although incorrect protein folding at low temperature is less expected, chaperons and<br />
proteases are required to deal with intracellular protein perturbations [30, 39]. This was<br />
confirmed by the induction of GroEL (RB8970) [25, 26] and htrA-protease (RB12752) in<br />
R. baltica as well. One of the most prominent responses of microorganisms to cold shock is<br />
the induction of cold shock proteins. However, the two annotated cold shock proteins of class<br />
I (CspA - RB4681 and Cspl - RB10009) were not regulated in the genome of R. baltica [40,<br />
41]. Consequently, the stabilization of RNA seems to employ a different set of proteins than<br />
in E. coli.<br />
High salinity<br />
As a marine organism, R. baltica needs to adjust to the haline stratification of the Baltic Sea<br />
[42, 43]. While wandering through the water column R. baltica cells are exposed to high, as<br />
well as changing concentrations of dissolved salts. In general, an osmotic up-shift forces<br />
bacteria to change their physiology by activating or deactivating specific enzymes or<br />
transporters, in order to maintain water balance [44]. To gain an understanding of the genetic<br />
events that occur during the early stages of salt adaptation, R. baltica cells were subjected to<br />
salt up-shock from 17.5‰ salinity (Baltic Sea) to 59.5‰ (hyper saline environment). Former<br />
experiments showed that R. baltica is able to grow between 4.2‰ and 59.5‰ salinity [2] and<br />
does not grow at the next tested salinity over 90‰ (Wohlrab, unpublished data).<br />
In total, 1127 genes showed differences in gene expression over the whole time series. 656 of<br />
these genes (58%) were annotated as hypothetical proteins. The salt up-shock results<br />
indicated an increase in the number of regulated genes over time. After 10 min 61 genes (1%)<br />
were regulated. The largest number (543 – 8%) was observed at 300 min (FIGURE 1 iii).<br />
R. baltica cells seem to adapt slowly to high salt concentration. This might be a result of the<br />
cell compartmentalization and the ability of R. baltica to temporarily resist higher salt<br />
concentration without significant adaptations.<br />
The response of R. baltica to salt stress includes repression of genes associated with the COG<br />
classes: induction of amino acid transport and metabolism [E], lipid metabolism [I],<br />
transcription [K], translation process [J]. Induced were genes involved in the already<br />
31
Results and Discussion<br />
mentioned classes of the heat shock experiment: [O], [M] and [L], but also in the energy<br />
production [C] and cell division and chromosome partitioning [D] classes (FIGURE 2 iii).<br />
Similar to other bacteria, R. baltica accumulated glutamate and trehalose as cytoplasmic<br />
osmoprotectants in response to osmotic stress [45]. Glutamate dehydrogenase (RB6930)<br />
showed an up-regulation already after 10 min and was also present in the proteome [26].<br />
Trehalose synthetase (treS - RB519) was induced after 60 min. Cysteine, as a general<br />
protective component, was only needed in the first hour in elevated concentrations. The<br />
synthesis was repressed after 1 hour (RB4386).<br />
The accumulation of compatible solutes is a widely distributed mechanism coping with<br />
environmental conditions [45, 46]. In R. baltica 74 planctomycetes-group-specific genes are<br />
annotated as hypothetical proteins carrying a Domain of Unknown Function (DUF) named<br />
1559 [19]. This domain belongs to a new family of solute binding proteins (PF07596) [47],<br />
and was also found on the planctomycete fosmid 8FN [19]. Nine of these genes were upregulated<br />
during the first hour in the cold and salt shock experiments, as opposed to the heat<br />
shock experiment where 16 of these genes were down-regulated. In vitro experiments have<br />
shown that some of these compatible solutes also possess general protein stabilizing<br />
properties beside their osmoprotective property [48]. These homologue proteins do not play<br />
an integral role in the transport process per se, but probably serve as receptors to trigger or<br />
initiate translocation of the solute through the membrane by binding to external sites of the<br />
integral membrane proteins of the efflux system. In addition, some solute-binding proteins<br />
function in the initiation of sensory transduction pathways [47].<br />
R. baltica up-regulated an efflux pump (RB7603) and a Na + /H + antiporter (RB1433) 300 min<br />
after salt shift. Both may play a role in the active export of salt ions out of the cells. Quinone<br />
oxidoreductase-like protein (RB10967), induced after 40 min, had been implicated in<br />
respiration-coupled Na + efflux as also shown in D. vulgaris [32]. Regulatory proteins like<br />
sigma-54 factor rpoN (RB6491), rpoA (RB12626) and rfaY (12251) were down-regulated.<br />
rpoN and rpoA were found to be translated [25, 26]. R. baltica inhibited the genes for cell<br />
division (soj - RB2291) and chromosome segregation (SMC - RB6065) after 60 min salt<br />
stress, as well as diverse transferases (RB12080, RB8898, RB12690, RB2498, RB8222,<br />
RB9617) involved in the cell envelope modification. Interestingly, the pilin transport<br />
apparatus and the thin-pilus basal body (pilM -RB2860 and pilT - RB12773) were induced<br />
after one hour, and principle pilus associated adhesion (pilC - RB12781) and pilB<br />
(RB12774). Also induced were genes coding for biopolymer transport proteins (exbB -<br />
RB12053 and exbD - RB12055). A homolog to exbD was annotated on the planctomycete<br />
32
Results and Discussion<br />
fosmid 3FN [19]. It is known from other organisms that genes encoding the flagellar and<br />
chemotaxis systems are up-regulated to move away from the stressful cations [32]. However<br />
none of the flagellar genes were regulated and the genome does not harbor any essential<br />
chemotaxis genes except cheY [16]. Notably, the survival protein (SurE -RB10258) and two<br />
genes coding for the mechanosensitive ion channel (MscS - RB12279 and RB10255) are<br />
induced. Latter provide protection against hypo-osmotic shock, responding both to stretching<br />
of the cell membrane and to membrane depolarization [49]. Genes in Cluster 22<br />
(ADDITIONAL FILE 2) seemed to be significantly affected by the salt stress.<br />
Common stress response<br />
R. baltica showed a common stress response to all three tested environmental factors. Several<br />
known general stress genes were induced, like genes coding for the manganese-containing<br />
catalase (RB10727), which is also present on the proteome [23, 25, 26]. Ferritin and Dps<br />
(RB4433) or pyridoxamine 5'-phosphate oxidase (RB4438) belong to a general stress cluster<br />
(RB4432-4438) and were initially described by Hieu et al. [26]. Thioredoxin (RB10378)<br />
could serve as an electron donor for the up-regulated methionine sulfoxide reductase gene<br />
(msrB - RB2268) [50, 51]. The genes could be regulated via rpoN based of the proposed<br />
upstream sigma 54-dependent promoter (RB10378) [52].<br />
To eventually cope with reactive oxygen species (ROS), typically present under stressful<br />
conditions [51], the nitrogen fixation protein (nifU - RB3596) was induced. NifU is involved<br />
in the biosynthesis and repair of ROS scavenging iron-sulfur clusters. Finally, the induced<br />
peptidase M50 (RB6092) could have been transcripted to regulate stress responses,<br />
sporulation, cell division, and cell differentiation [53].<br />
Genes involved in the fatty acid metabolism were repressed under all conditions in R. baltica.<br />
Examples are genes like CoA-acyl carrier protein transacylase (fabD - RB314), the acyl<br />
carrier protein (acpP - RB318) and the fabB (RB320) gene.<br />
Interestingly, the machinery for rearrangement and the interchange of the genetic material<br />
was induced under all three stressful conditions. It seems to play an important role in the<br />
organism’s long-term adaptation. R. baltica harbors 81 non-randomly distributed<br />
transposases in its genome. Noteworthy, under heat stress three times more transposase genes<br />
was up-regulated than under cold stress and twice as many as under salt stress. Shared<br />
induction shows five IS3/IS911, three ISXo8, two putative transposases (RB170, RB5888,<br />
RB11749, RB11802, RB12940, RB2186, RB9907, RB12239, RB934 and RB7389), and one<br />
33
Results and Discussion<br />
integrase (RB11750). Rearranging the genome to select the most efficient gene combination<br />
has been described as a common way to adapt quickly to extreme environments [36].<br />
Relaxed DNA may also be required to get better access to the gene regions for increased<br />
expression. This is indicated by the repressed histone-like DNA-binding protein (RB6276).<br />
In line with an alternative global sensing and regulation system initially proposed by<br />
Glöckner et al. [16], a common sensing and regulation was found. R. baltica contains 37<br />
genes belonging to the extracytoplasmic function (ECF) subfamily of sigma 70 [54]. The<br />
genes RB138, RB13241 and RB10049 are up-regulated under all three stressful conditions.<br />
Studholme et al. [47] suggests that ECF-factor RB10049 is the regulator for the conserved<br />
hypothetical protein RB10051. The conserved domain belongs to a new group of proteins<br />
that share novel domains referred to as planctomycete-specific (PSD) or planctomycetespecific<br />
cytochrome C (PSC). RB10051 contains the PSD1 (DUF1553 - PF07587) and PSC2<br />
(DUF1549 - PF07583) domains suggestion a function in redox reactions [47]. Each domain is<br />
represented 41 times in the whole genome of R. baltica [19].<br />
Additionally, at 300 min the ECF-sigma factor RB138 was up-regulated together with<br />
serine/threonine protein kinase (RB140). Protein kinases are believed to be involved in the<br />
stress response [38, 55]. The serine/threonine protein kinase (RB12942) and two histidinekinases<br />
(RB4511 and RB10330) were up-regulated during heat shock. Whereas, under cold<br />
shock only one serine/threonine kinase (RB8505) was induced. Under salt stress a histidinekinase<br />
(RB13122) and three two-component systems (RB5780, RB12952 and RB13118)<br />
were induced.<br />
Finally, the ECF-sigma factor RB1790 was up-regulated, but only under high salinity<br />
conditions. In summary, the results confirmed that ECF sigma factors, as well as twocomponent<br />
systems, are heavily involved in stress sensing and regulation of R. baltica. The<br />
importance of these genes in the natural environment is indicated by the presence of a<br />
homolog to RB12952 on the planctomycete fosmid 6N14 [19].<br />
The down-regulation of genes associated with the ribosomal machinery (55%) was observed.<br />
During heat shock and high salinity they were permanently repressed, whereas under cold<br />
shock they were only repressed within the first hour. Out of 51 ribosomal proteins in the<br />
whole genome, 18 genes encoding proteins of the small- and large subunit (RB1233,<br />
RB12821, RB12824, RB12839, RB7117, RB7837 - RB7841, RB7849, RB7850, RB7852,<br />
RB7854, RB7856, RB7857, RB7859 and RB7899) were repressed. Additionally, a set of<br />
genes involved in RNA metabolism, protein synthesis, as well as the only translation<br />
elongation factor (EF-Tu - RB7894) in R. baltica were repressed. The genes for the<br />
34
Results and Discussion<br />
conserved hypothetical protein RB12818 and the hypothetical protein RB12837 were coregulated<br />
which indicates an association with the translation machinery. The repression of the<br />
ribosomal genes, along with a large set of genes involved in RNA metabolism, protein<br />
synthesis, cell growth (Cluster 1 ADDITIONAL FILE 2), has been reported as a general<br />
feature of the environmental stress responses (ESR) [35]. It is assumed that they are acting as<br />
stress sensors [56]. This coincides nicely to the induction of the ribosomal proteins at<br />
300 min under cold shock conditions. Recovery and ongoing adaptation of R. baltica was<br />
further supported by the up-regulation of the ribosomal-binding factor rbfA (RB5503), which<br />
is, besides csdA, required for optimal growth at low temperatures [57].<br />
Hypothetical proteins<br />
Approximately 50% of the regulated genes have no known function in each of the three<br />
environmental stress experiments. Some of them even share a similar expression profile<br />
(TABLE 2 and TABLE 3). We propose that some of these genes are involved in cell<br />
morphology changes, stress sensing and regulation. The low number of known transcriptional<br />
regulators (2.4%) in the genome of R. baltica [54], and the fact that most of the essential<br />
pathways are not organized in operon structures [16] support the hypothesis of novel kinds of<br />
global regulation mechanisms. Candidates are hypothetical proteins that carry regulatory<br />
domains, like the FHA domain in RB1789 or a putative transcriptional regulatory domain in<br />
RB9999. RB11766 might regulate the gene next to it, which is a so called giant gene<br />
(RB11769) [58]. This giant gene carries a novel peptide motif that is most likely involved in<br />
cell morphology changes [47]. The importance of the hypothetical proteins RB11505,<br />
RB10954, RB10956 and RB10958 was further confirmed by their presence on the proteome<br />
gels of Hieu et al. as well as Gade et al. [23, 25, 26]. The latter three of these genes were<br />
claimed to be one of the most abundant proteins when R. baltica cultures are grown on<br />
mineral medium.<br />
Planctomycete special feature: Genes encoding sulfatases<br />
The genome of R. baltica contains no less than 110 sulfatases. It is assumed that they are<br />
involved in the recycling of carbon from complex sulfated heteropolysaccharides. Although,<br />
the mineral medium does not contain any sulfated polysaccharide, we found 11 sulphatase<br />
genes up- or down-regulated (TABLE 1) including one choline sulphatase (RB1205), seven<br />
arylsulfatases (RB13148, RB1477, RB3403, RB406, RB5146, RB684 and RB9498), two<br />
35
Results and Discussion<br />
sulphatase genes without specificity (RB3956, RB5294), and one alkylsulfatase (RB11502)<br />
within the different stress experiments. Furthermore, during life cycle experiments<br />
(unpublished data) we found evidence that certain sulfatases are only regulated in specific<br />
growth stages which indicates their involvement in the remodeling of the distinct<br />
morphological features of R. baltica. RB1477, RB5294, RB9498 and RB11502 were<br />
induced. We propose a RB9498 and RB11502 have an extracellular function and may be<br />
involved in the formation of a protective substance.<br />
Six sulfatase genes (RB406, RB684, RB1205, RB3403, RB5146 and RB13145) were<br />
repressed after 300 min of heat shock. They could have been involved in the rearrangement<br />
of the cell wall formation, which consists of a protein sacculus with disulfide bonds [12]. In<br />
summary, these results show again how diverse the role of the sulfatases might be and that<br />
only a variety of different experiments will lead to more knowledge on the sulfatases.<br />
Conclusion<br />
This work presents the first transcriptome study of the environmental stress response of a<br />
marine, free-living Planctomycete. Although R. baltica is an unusual organism in many<br />
aspects, the stress responses to heat and cold shock as well to changing salinity were in line<br />
with earlier results reported for other model organisms. Heat shock induced a set of<br />
chaperons, likely to protect cellular proteins from denaturation and breakdown. Growth in the<br />
cold can be followed by the induction of genes altering lipid metabolism. Salinity shift<br />
resulted in the activation of a planctomycete-specific groups of genes, genes involved in<br />
morphology change and the induction of an extracytoplasmatic stress response. All stressors<br />
triggered the down-regulation of the ribosomal machinery, the up-regulation of transposases<br />
to enhance the genetic activity and the induction of several ECF-sigma factors and twocomponent<br />
systems. This confirms the hypothesis that R. baltica is regulating its gene<br />
activity on a global- rather than on the operon level. Besides well characterized stress<br />
response genes about 1000 genes of unknown function, constituting 13 % of the genes<br />
predicted in the genome, were affected. Among them a set of genes most likely involved in<br />
global regulation could be proposed. This in combination with proteome studies and the<br />
presence of some of the genes in fosmid libraries confirmed the essential role of the vast<br />
amount of genes with so far unknown functions in the adaptation of environmental organisms<br />
to changing conditions. Noteworthy is the regulation of 11 sulfatases during stressful<br />
conditions, which indicates that these genes are heavily involved in the core functions<br />
36
Results and Discussion<br />
exposed by R. baltica. The data presented lead to the conclusion that R. baltica’s rich<br />
repertoire of genes is combined with a fine tuned regulation mechanism to best adapt to the<br />
permanent changing conditions in its habitat. This work lays the foundation to get a deeper<br />
understanding of the biology and behavior of Planctomycetes, a fascinating and diverse<br />
phylum.<br />
Methods<br />
Bacterial growth conditions<br />
For all experiments Rhodopirellula baltica SH 1 T cells were grown as chemostat cultures in a<br />
mineral medium containing 10 mM glucose as sole carbon and 1 mM ammonium chloride as<br />
nitrogen source at 28°C [21]. Chemostate (Ø 13.5 cm x 25 cm, 1 l, Schott, modified by Ochs,<br />
Bovenden) parameters were: pH 7.4, average dilution rate 0.75 ml/min and pO 2 around<br />
100%. The cultures had an OD 600nm of 0.5 – 0.6 (corresponding to log phase). The cells were<br />
harvested after 5 dwell times.<br />
Sample collection, cell lyses, RNA Isolation and cDNA synthesis<br />
After harvesting an aliquot was collected to serve as the time-zero reference. The culture<br />
broth was collected in 500 ml tubes and swirled briefly in an ethanol-dry ice bath to rapidly<br />
cool the cultures and prevent shifts in the RNA profile. Subsequently, the broth was<br />
centrifuged at 6000 rpm for 20 min at 4°C (Beckman Coulter TM AvantiTM626 J-20XP, JA10<br />
Rotor). The pellets were re-suspended in 0.1 M Tris-HCL and then re-centrifuged to cell<br />
pellets that were shock-frozen in liquid nitrogen and stored at -80°C. Total RNA was isolated<br />
using the protocol of the TRI Reagent ® Kit by Ambion (Austin, USA). The purity and quality<br />
of the extracted total RNA was checked with an Agilent 2100 Bioanalyzer (Agilent<br />
Technologies, Palto Alto, USA) and gel electrophoresis. cDNA synthesis was performed<br />
using the SuperScript direct cDNA labeling kit by Invitrogen (Karlsruhe, Germany)<br />
according to the manufacturer´s instructions with random hexamers and unlabeled<br />
dCTP/dUTP, followed by an three hours reverse transcription incubation step at 46°C and 3<br />
min at 95°C to stop the RT reaction. To hydrolyze the RNA 0.1 M NaOH was added,<br />
incubated at 65°C for 15 min and neutralized with 0.1 M HCL. The remaining cDNA was<br />
precipitated over night at -20°C and the pellet was washed with 70% Ethanol.<br />
37
Results and Discussion<br />
cDNA was directly labeled using the PlatinumBright TM nucleic acid labeling kit based on<br />
KREATECH´s patented Universal Linkage System (ULS) (Biocat, Heidelberg, Germany)<br />
according to the manufacture’s protocol.<br />
Concentrations of RNA and cDNA were measured, and incorporation of the dyes Alexa 546<br />
and Alexa 647 were checked, using a Nanodrop ND-1000 spectrophotometer (NanoDrop<br />
Technologies, Wilmington, USA).<br />
Experimental design and sample preparation<br />
In three independent hybridizations for each experiments and time point the expression<br />
profiles of cells that had undergone stress were compared with those of cells at time zero.<br />
This means that for the array analysis each Alexa 647 labeled sample was compared with<br />
Alexa 546 labeled time-zero samples. The data shown are based on the analysis of all three<br />
replicates performed for each of the conditions.<br />
For expression profiling and microscopic analysis samples were collected at 10, 20, 40, 60<br />
and 300 min for all three stress experiments.<br />
Heat shock from 28°C to 37°C<br />
Cells grown continuously at 28°C were collected by centrifugation. An aliquot was removed<br />
for RNA extraction and taken as the time zero reference for the heat, cold and salt stress<br />
experiments. Aliquots were re-suspended in an equal volume of 37°C medium and returned<br />
to 37°C for cultivation.<br />
Cold shock from 28°C to 6°C<br />
Cells grown continuously at 28°C were collected by centrifugation, re-suspended in an equal<br />
volume of 6°C medium and returned to 6°C for cultivation.<br />
Salt stress from 17.5‰ to 59.5‰ salinity<br />
Similar to the heat and cold shock experiments, a R. baltica culture was grown in mineral<br />
media with 17.5‰ salinity. Cells were harvested and aliquots were taken up in a mineral<br />
media with a salinity of 59.5‰.<br />
38
Results and Discussion<br />
<strong>Whole</strong> <strong>Genome</strong> <strong>Array</strong> construction, hybridization and image analysis<br />
The whole-genome oligonucleotides for R. baltica SH 1 T (Pirellula AROS 630 Version 1.0)<br />
were purchased from Operon (Cologne, Germany) and diluted to 20 μM concentration in<br />
Micro Spotting Solution Plus spotting buffer (Telechem, Sunnyvale, USA). Spotting was<br />
done with three replicates per gene and slide onto GAPS II aminosilane slides (Corning,<br />
Schiphol-Rijk, Netherlands) using a Spot<strong>Array</strong> 24 spotting device (Perkin Elmer, Wellesley,<br />
USA) together with 48 Telechem Stealth Pins (Telechem, Sunnyvale, USA). The arrays were<br />
subsequently exposed at 245 nm and 360 mJ in the GS Gene Linker (Bio-Rad, München,<br />
Germany), followed by incubation at 80°C for at least 3 h. Slides were stored at room<br />
temperature in the dark until use.<br />
Blocking, denaturing, hybridization, washing and drying of the slides with N 2 were carried<br />
out in an automated hybridization station HS400 (Tecan, Crailsheim, Germany). The spotted<br />
arrays were blocked in prehybridization solution containing 250 mM NaCl, 5 mM Tris/HCl<br />
at pH 8.0, 50% formamide, 0.5x SSC, 0.05% BSA, and 1% blocking reagent from Roche<br />
Diagnostics, Mannheim, Germany for 45 min at 52°C. For hybridization at least 2 μg of<br />
Alexa 546 dye-labeled and 2 μg of Alexa 647 dye-labeled total cDNA were combined and<br />
taken up in a final volume of 100 µl DIG Easy Hyb hybridization solution (Roche<br />
Diagnostics, Mannheim, Germany). After the blocking step the sample solution was applied<br />
to the arrays, denaturized at 95°C for 3 min and hybridized under stringent conditions at<br />
52°C for over 12 hours. After hybridization slides were washed at room temperature in<br />
ULTR<strong>Array</strong> Low Stringency Wash Buffer (Ambion, Austin, USA) and dried by N 2 .<br />
Signal detection and data analysis<br />
Slides were scanned at a resolution of 5 μm using a Scan<strong>Array</strong> Express Microarray scanner<br />
(Perkin Elmer, Wellesley, USA) with varied laser power and sensitivity level of the<br />
photomultiplier tube (PMT) for each slide. The provided image analysis software Scan<strong>Array</strong><br />
Express Version 4.0 was used for automatic spot detection and signal quantification of both<br />
fluorophores. Raw data were automatically processed using the in-house developed<br />
microarray data analysis software tool MADA (www.megx.net/mada). First of all the spot<br />
intensities were local background corrected (mean spot intensity minus mean spot<br />
background intensity). Then signals were only assessed as positive if mean spot pixel<br />
intensity was higher than the mean local background intensity plus two times the standard<br />
deviation of the mean local background pixel intensity. Each gene is spotted in three<br />
39
Results and Discussion<br />
replicates. Spot replicates with poor quality were removed from the data set according to the<br />
outlier test of MADA. For this test, first the standard deviation of all replicates is computed.<br />
Second, one replicate is omitted and the standard deviation is recalculated, if the deviation<br />
differs more than 50% from the previous deviation, the omitted replicate is regarded as an<br />
outlier. This procedure is alternately repeated for all replicates<br />
The expression is described through the ratio and intensity, where R is the fluorescence log<br />
ratio of the experiment time point relative to the control condition (e.g. R = log2 (result of<br />
channel 10min / result of channel control/reference)) and I is the log mean fluorescence<br />
intensity (e.g. I = log10 (result of channel 10min x result of channel control/reference)).<br />
Each data point represents a regulation factor (ratio) in a logarithmic scale for one gene<br />
calculated from the positive replicates for a particular probe coming from two RNA pools<br />
(reference and sample). Normalization was carried out by LOWESS fitting on an R-versus-I<br />
plot with a smoothing factor of 0.5. Each time point of the time-series experiment was<br />
hybridized independently three times. The expression data (ratio) of the three hybridizations<br />
were combined to one expression data point (ratio) by average and the standard deviation was<br />
calculated. Only ratios with a standard deviation less than 25% were taken as regulated.<br />
Differentially expressed genes are determinate by a fixed threshold cut off method (i.e. a twofold<br />
increase or decrease) based on the self-self hybridization. Taking the same biological<br />
sample the reference is labeled twice, once with Alexa 546 and once with Alexa 647 and the<br />
variability between the two sets of measurements are calculated to estimate the experimental<br />
noise. Ideally there should not be any variability and all expression points should have a ratio<br />
close to zero. In reality this is never the case and thresholds on the basis of the distribution of<br />
these data along the y-axis were defined for the further experiments.<br />
Consequently, R. baltica genes detected with intensities resulting in ratios above or below<br />
these thresholds can be regarded as up- or down-regulated.<br />
Cluster analysis<br />
Differentially expressed genes presenting the complete time course profile (10, 20, 40, 60<br />
und 300 min) for all three experiments were clustered using the k-means clustering approach<br />
(Euclidean distance metric, k = 30 clusters and 49 (max. 500) iterations) [59] with the<br />
software tool Multiexperiment Viewer MeV Version 4.0.2 from the TM4 microarray<br />
software suite [60]. Briefly, the clustering algorithm arranges genes into a given number of<br />
clusters k according to their similarity in expression profiles across all of the array<br />
40
Results and Discussion<br />
experiments, such that genes with similar expression patterns are clustered together. The data<br />
are graphically displayed in tabular format in which each row of colored boxes represents the<br />
variation on transcript abundance for each gene and each column represents the variation in<br />
transcript levels of every gene in a given mRNA sample, as detected on one array. The<br />
variations in transcript abundance for each gene are depicted by means of a color scale, in<br />
which shades of red represents increases and shades of green represent decrease in mRNA<br />
levels, relative to the unstressed culture, and the saturation of the color corresponds to the<br />
magnitude of the differences. A black color indicates no change in transcript level, and a grey<br />
color represents missing data.<br />
<strong>Genome</strong> tools<br />
The genome of Rhodopirellula baltica was automatically re-annotated based on updated<br />
homology searches (June 2005 - MicHanThi [61]). The updated annotation including all tool<br />
results are publicly available [62]. JCoast [63] was used as a tool for the visualization,<br />
interpretation, COG-assignment statistics and comparison of genomic data stored in GenDB<br />
V2.2 [64]. The Venn diagrams were generated by BioVenn [65].<br />
Microarray Datasets<br />
Each microarray used in this study contained 7325 known or predicted R. baltica genes<br />
according to Glöckner et al. [16]. A detailed description of the array can be found at the<br />
NCBI´s Gene Expression Omnibus (GEO) database under accession number GPL7654. The<br />
complete microarray datasets covering the expression of R. baltica cultures exposed to heat,<br />
cold and high salinity, are public available in the GEO repository<br />
(http://www.ncbi.nlm.nih.gov/geo/) under accession numbers GSE13769, GSE13856 and<br />
GSE14075 [66].<br />
List of abbreviations<br />
COG Cluster of Orthologous Group of Genes<br />
DUF Domain of Unknown Function<br />
ECF Extra Cytoplasmic Function<br />
ERS Environmental Stress Response<br />
FHA Forkhead-associated<br />
GEO Gene Expression Omnibus<br />
41
Results and Discussion<br />
R. Rhodopirellula<br />
RB Rhodopirellula baltica<br />
ROS Reactive Oxygen Species<br />
ORF Open Reading Frame<br />
Competing interests<br />
The authors declare that they have no competing interests.<br />
Authors contribution<br />
PW conceived the study, initiated, conducted the experimental analysis, validated microarray<br />
and optimized experimental steps, wrote the manuscript, did the statistical analysis and<br />
analyzed the data.<br />
CK was involved in the chemostat cultivation of R. baltica and statistical analysis of the data<br />
AE wrote MADA the microarray analysis tool and designed the microarrays.<br />
CQ was responsible for the automatic reannotation of the genome and set up the web access<br />
PL and JH established the chemostat cultivation of R. baltica. JH supervised the chemostat<br />
cultivation.<br />
FOG contributed background information and was involved in writing and finishing the<br />
manuscript.<br />
All authors read and approved the final manuscript.<br />
Acknowledgements<br />
We thank Sylke Wohlrab for preliminary work and Matthias Gottschall for excellent<br />
technical assistance in the chemostat cultivation.<br />
The setup of the microarray and transcriptomic analysis of Rhodopirellula baltica was<br />
supported by European Commission contract GOCE-CT-2004-505403.<br />
42
Results and Discussion<br />
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46
Results and Discussion<br />
Figure legends<br />
800<br />
700<br />
600<br />
i)<br />
500<br />
400<br />
329<br />
428<br />
300<br />
200<br />
100<br />
171<br />
219<br />
180<br />
146<br />
165<br />
192 220<br />
322<br />
0<br />
800<br />
ii)<br />
Number of regulated genes<br />
700<br />
600<br />
500<br />
400<br />
300<br />
200<br />
100<br />
0<br />
800<br />
41<br />
38<br />
274<br />
145<br />
46<br />
50 66<br />
75 94 93<br />
700<br />
iii)<br />
600<br />
500<br />
400<br />
226<br />
300<br />
200<br />
100<br />
0<br />
138<br />
317<br />
14<br />
213<br />
27<br />
32<br />
44<br />
29<br />
95<br />
10 min 20 min 40 min 60 min 300 min<br />
FIGURE 1 Number of regulated genes per stress experiment<br />
Each column shows the total number of up- (gray) and down- (white) regulated genes at each taken time<br />
points compared with the reference samples. i) heat shock, ii) cold shock and iii) high salinity<br />
47
Results and Discussion<br />
120<br />
100<br />
80<br />
i)<br />
60<br />
40<br />
20<br />
0<br />
C D E F G H I J K L M N O P Q R S T<br />
120<br />
ii)<br />
Number of regulated genes<br />
100<br />
80<br />
60<br />
40<br />
20<br />
0<br />
C D E F G H I J K L M N O P Q R S T<br />
120<br />
100<br />
iii)<br />
80<br />
60<br />
40<br />
20<br />
0<br />
C D E F G H I J K L M N O P Q R S T<br />
Functional class designation<br />
FIGURE 2 Number of regulated genes with an assigned COG-category<br />
48
Results and Discussion<br />
Each column shows the number of up- (gray) and down- (white) regulated genes per an assigned COGcategory<br />
according to the NCBI database (cut off e-value e-4)<br />
i) heat shock, ii) cold shock and iii) high salinity; Columns: [C] Energy production and conversion, [D]<br />
Cell division and chromosome partitioning, [E] Amino acid transport and metabolism, [F] Nucleotide<br />
transport and metabolism, [G] Carbohydrate transport and metabolism, [H] Coenzyme metabolism, [I]<br />
Lipid metabolism, [J] Translation, ribosomal structure and biogenesis, [K] Transcription, [L] DNA<br />
replication, recombination and repair, [M] Cell envelope biogenesis, outer membrane, [N] Cell motility<br />
and secretion, [O] Posttranslational modification, protein turnover, chaperones, [P] Inorganic ion<br />
transport and metabolism, [Q] Secondary metabolites biosynthesis, transport and catabolism, [R]<br />
General function prediction only, [S] Function unknown, [T] Signal transduction mechanisms<br />
Repressed<br />
Induced<br />
All time points<br />
Heat<br />
83<br />
78<br />
Cold<br />
90<br />
17<br />
274<br />
85<br />
106<br />
Salinity<br />
Heat<br />
48<br />
39<br />
Cold<br />
62<br />
263<br />
110<br />
293<br />
31<br />
Salinity<br />
FIGURE 3 Venn diagrams of specific and common stress response<br />
The diagram shows the distribution of stimulus-specific and common stress responses. All genes of all<br />
time points are represented in one diagram divided into repressed and induced genes<br />
49
Results and Discussion<br />
TABLE<br />
TABLE 1 Differentially expressed sulfatase genes of R. baltica<br />
ID Product AA<br />
Signal<br />
P<br />
Heat Cold Salt Remarks<br />
RB1205 choline sulfatase 456 0.8 repressed repressed<br />
RB3403<br />
arylsulfatase<br />
precursor<br />
491 0.99 repressed repressed [25, 26]<br />
RB3956 sulfatase 489 0.98 repressed<br />
RB5146<br />
arylsulfatase<br />
precursor (ASA)<br />
A<br />
522 0.95 repressed<br />
RB9498 arylsulfatase A 518 0.97 induced [25, 26],<br />
RB11502<br />
alkyl sulfatase or<br />
beta-lactamase<br />
445 1 induced<br />
RB1477<br />
arylsulfatase<br />
precursor<br />
538 0 induced induced<br />
RB5294 sulfatase 533 0 induced<br />
wall unpublished<br />
results, [67]<br />
RB406 arylsulfatase 557 0 repressed repressed<br />
RB684<br />
arylsulfatase<br />
precursor<br />
653 0 repressed repressed [67]<br />
RB13148<br />
arylsulfatase<br />
[precursor]<br />
A<br />
1012 / repressed [67]<br />
50
Results and Discussion<br />
TABLE 2 Shared stress response: Selection of the induced genes<br />
ID AA Product IEP Strand Potentially involved in /<br />
Comments<br />
RB170 96 Transposase IS3/IS911 10.1 +<br />
RB370 553 nitrate transporter substrate-binding 4.6 +<br />
protein<br />
RB521 63 hypothetical protein 10.7 - [25, 26]<br />
RB723 60 hypothetical protein 7.3 +<br />
RB934 375 Putative transposase 9.9 -<br />
RB1394 78 hypothetical protein 10.1 - regulatory mechanism<br />
RB1395 319 secreted protein similar to DNAbinding<br />
5.5 + regulatory mechanism<br />
protein<br />
RB1789 243 conserved hypothetical protein 9.4 + regulatory mechanism<br />
RB1872 38 hypothetical protein 12.3 +<br />
RB2186 433 ISXo8 transposase 9.4 -<br />
RB2268 282 peptide methionine sulfoxide reductase 9.9 - [23]<br />
RB3596 144 nitrogen fixation protein (NifU protein) 4.3 +<br />
RB4299 96 Transposase IS3/IS911 9.9 +<br />
RB4347 156 conserved hypothetical protein 4.7 + [25, 26]<br />
RB4397 55 protein containing DUF1560 10.5 +<br />
RB4429 89 conserved hypothetical protein 4.9 + stress response<br />
RB4433 162 Ferritin and Dps 4.3 +<br />
RB4438 160 Pyridoxamine 5'-phosphate oxidase- 4.4 +<br />
RB4510 49 hypothetical protein 9 -<br />
RB5238 73 hypothetical protein 10.6 +<br />
RB5551 663 hypothetical protein 5.7 + PFAM:DVL<br />
RB5888 96 Transposase IS3/IS911 10.1 -<br />
RB5938 370 hypothetical protein- 5.6 - [25]<br />
RB6928 160 hypothetical protein 4.1 +<br />
RB7389 375 Putative transposase 9.9 +<br />
RB8409 97 hypothetical protein 8.9 +<br />
RB8527 330 protein containing DUF1559 6.6 + stress response<br />
RB8987 48 hypothetical protein 9.1 -<br />
RB9230 107 hypothetical protein 9.9 + next to transposase<br />
RB9907 433 ISXo8 transposase 9.4 +<br />
RB9955 452 secreted protein containing DUF1552 5.6 - regulatory mechanism<br />
RB9999 281 conserved hypothetical protein- 4.6 - regulatory mechanism<br />
RB10049 217 RNA polymerase ECF-type sigma 10.1 +<br />
factor<br />
RB10378 144 Thioredoxin 4.6 -<br />
RB10727 276 manganese-containing catalase 5 + [25, 26]<br />
RB10728 132 secreted protein 9.9 + stress response<br />
RB10896 161 secreted protein 10 - stress response<br />
RB10954 143 hypothetical protein 10.4 -<br />
RB10956 117 hypothetical protein 4.8 + [26]<br />
RB10957 99 conserved hypothetical protein 5.6 + regulatory mechanism<br />
RB10958 158 hypothetical protein 5.4 +<br />
RB11176 153 protein containing DUF442 4.8 - [26] stress response<br />
RB11260 121 dnaK suppressor protein, 5.2 -<br />
RB11475 57 conserved hypothetical protein 4.7 + next acyltranscferase, short<br />
protein<br />
RB11504 72 conserved hypothetical protein 10.7 - short protein,<br />
RB11505 199 conserved hypothetical protein, secreted 7.5 -<br />
RB11515 74 conserved hypothetical protein 11.7 +<br />
RB11566 195 hypothetical protein 10.8 +<br />
RB11749 96 Transposase IS3/IS911 10.1 +<br />
RB11750 292 integrase 10 +<br />
RB11802 96 Transposase IS3/IS911 10.1 +<br />
RB11855 101 conserved hypothetical protein 12.5 -<br />
RB11918 134 protein containing DUF971 6.1 -<br />
51
Results and Discussion<br />
RB11977 196 conserved hypothetical protein 9.5 +<br />
RB12066 135 hypothetical protein 10 +<br />
RB12239 433 ISXo8 transposase 9.4 +<br />
RB12247 74 conserved hypothetical protein 6.3 -<br />
RB12936 580 conserved hypothetical protein 5.3 - DUF 444<br />
RB12940 96 Transposase IS3/IS911 10.1 +<br />
RB13222 208 SOUL heme-binding protein 8.7 -<br />
RB13241 167 RNA polymerase ECF-type sigma 8.8 -<br />
factor<br />
52
Results and Discussion<br />
TABLE 3 Shared stress response: Selection of the repressed genes<br />
ID AA Product IEP Strand<br />
Potentially involved in /<br />
Comments<br />
RB61 58 hypothetical protein 9.6 +<br />
RB314 309<br />
malonyl CoA-acyl carrier protein<br />
transacylase<br />
4.4 +<br />
RB318 81 Acyl carrier protein 3.7 +<br />
RB319 95 hypothetical protein 10.2 + fatty acid process<br />
RB767 311 conserved hypothetical protein, secreted 5.7 +<br />
RB825 117 hypothetical protein 7.8 -<br />
RB951 234 protein containing DUF1596 12.3 -<br />
RB1129 895 conserved hypothetical protein 5.8 -<br />
RB1233 206 30S ribosomal protein S4 11.2 -<br />
RB2105 470 membrane protein 9.6 -<br />
RB2306 41 hypothetical protein 9 +<br />
RB2479 273 conserved hypothetical protein 5.5 -<br />
RB3277 221 hypothetical protein 10.4 -<br />
RB3362 87 hypothetical protein 11.9 -<br />
RB3366 78 hypothetical protein 5.6 +<br />
RB3394 36 hypothetical protein 10.5 +<br />
RB3399 65 hypothetical protein 9.7 -<br />
RB3575 152 membrane protein 10.1 + [22]<br />
RB3603 344 secreted protein 4.6 -<br />
RB3675 742 secreted protein 8.4 +<br />
RB3688 53 hypothetical protein 9.3 -<br />
RB3880 82 hypothetical protein 10.7 -<br />
RB3953 857 hypothetical protein 5.2 -<br />
RB3981 161 hypothetical protein 4.1 +<br />
RB3994 191 hypothetical protein 4.1 +<br />
RB4097 733 conserved hypothetical protein 6.2 +<br />
RB4145 90 hypothetical protein 12 -<br />
RB4194 53 hypothetical protein 11.4 -<br />
RB4269 282 glutamic acid specific endopeptidase 5.6 -<br />
RB4358 123 hypothetical protein 6.5 - [25, 26]<br />
RB4373 109 hypothetical protein 4.8 -<br />
RB4657 123 hypothetical protein 12.2 +<br />
RB4951 95 hypothetical protein 12.1 +<br />
RB5262 95 membrane protein 6.3 +<br />
RB5409 97 hypothetical protein 12.7 +<br />
RB5415 62 hypothetical protein 12.3 +<br />
RB5745 130 hypothetical protein 10.7 -<br />
RB6092 361 Peptidase M50 9.6 +<br />
RB6158 142 hypothetical protein 6 -<br />
RB6174 69 hypothetical protein 10.6 -<br />
RB6276 105<br />
Histone-like bacterial DNA-binding<br />
10.4 -<br />
(S) bombinin, defense<br />
response<br />
next to a seronine-threonine<br />
kinase<br />
genetic<br />
processing<br />
protein<br />
RB6634 365 protein containing DUF1559 5.3 +<br />
RB6699 47 hypothetical protein 11.1 +<br />
RB6766 55 hypothetical protein 12 +<br />
RB6849 101 hypothetical protein 12.8 -<br />
RB7042 91 hypothetical protein 10.4 +<br />
RB7116 59 hypothetical protein 11.7 + ribosomal machinery<br />
RB7117 181 Ribosomal protein L35 11.4 -<br />
RB7557 327<br />
von Willebrand factor type A domain<br />
protein<br />
4.9 +<br />
RB7646 62 hypothetical protein 10.5 +<br />
RB7647 73 hypothetical protein 7.4 +<br />
information<br />
53
Results and Discussion<br />
RB7837 286 Ribosomal protein L2 11.8 +<br />
RB7838 89 Ribosomal protein S19/S15 10.8 +<br />
RB7839 119 Ribosomal protein L22/L17 11 +<br />
RB7840 236 30S ribosomal protein S3 10.4 +<br />
RB7841 138 Ribosomal protein L16 11.1 +<br />
RB7849 108 Ribosomal protein S17 10 +<br />
RB7850 122 Ribosomal protein L14b/L23e 11 +<br />
RB7852 196 50S ribosomal protein L5 10.4 +<br />
RB7854 61 Ribosomal protein S14 11.8 +<br />
RB7856 181 50S ribosomal protein L6 10 +<br />
RB7857 149 Ribosomal protein L18P/L5E 11.6 +<br />
RB7859 177 Ribosomal protein S5 10.6 +<br />
RB7894 398 translation elongation factor EF-Tu 5.2 + [25, 26]<br />
RB7899 141 50S ribosomal protein L11 9.6 +<br />
RB8119 142 hypothetical protein 10.1 +<br />
RB8457 113 hypothetical protein 11.5 -<br />
RB8594 41 hypothetical protein 9.2 +<br />
RB8669 37 hypothetical protein 11.5 + ribosomal machinery<br />
RB9343 59 hypothetical protein 11.4 +<br />
RB9417 103 hypothetical protein 10.5 +<br />
RB9460 79 hypothetical protein 10.3 -<br />
RB9872 67 hypothetical protein 5.4 + cell division related<br />
RB10581 384 secreted protein containing DUF1559 6.2 + [26]<br />
RB11287 75 hypothetical protein 9.1 +<br />
RB11392 148 conserved hypothetical protein 5 +<br />
RB11490 181<br />
conserved hypothetical protein,<br />
membrane<br />
10.3 +<br />
RB11707 83 conserved hypothetical protein 9.8 + stress function<br />
RB11766 129 hypothetical protein 10.4 + [47]<br />
RB12193 36 hypothetical protein 7.5 -<br />
overlapping with asnB<br />
(RB12191)<br />
RB12251 567<br />
RNA polymerase specialized sigma<br />
factor<br />
9.4 -<br />
RB12327 686<br />
TGF-beta receptor, type I/II<br />
extracellular region<br />
4.5 -<br />
RB12329 110<br />
conserved hypothetical protein,<br />
membrane<br />
4 +<br />
RB12396 57 hypothetical protein 11.3 -<br />
RB12454 199 hypothetical protein 10.3 -<br />
RB12818 163 conserved hypothetical protein 11.7 - ribosomal machinery<br />
RB12821 117 Ribosomal protein L19 11.1 -<br />
RB12824 146 Ribosomal protein S16 5.3 -<br />
RB12837 65 hypothetical protein 9.8 + ribosomal machinery<br />
RB12839 225 Ribosomal protein L1 9.8 +<br />
Additional files<br />
ADDITIONAL FILE 1 - List of differentially expressed genes for all three stress experiments<br />
ADDITIONAL FILE 2 - List of selected clusters of the k-means clustering<br />
54
Results and Discussion<br />
3.2 The Rhodopirellula baltica life-cycle: Growth-dependent<br />
expression profiling of the complete genome with microarrays<br />
Authors: Patricia Wecker, Marga Schüler, Andreas Ellrott, and Frank Oliver Glöckner<br />
Manuscript in preparation<br />
55
Results and Discussion<br />
The Rhodopirellula baltica life cycle: Growth-dependent<br />
expression profiling of the complete genome with<br />
microarrays<br />
Patricia Wecker 1,2 , Margarete Schüler 3 , Andreas Ellrott 1 and Frank Oliver Glöckner 1,2§<br />
1 Max Planck Institute for Marine Microbiology, Microbial Genomics Group, Celsiusstr. 1,<br />
28359 Bremen, Germany<br />
2 <strong>Jacobs</strong> <strong>University</strong> Bremen gGmbH, Campusring 1, 28759 Bremen, Germany<br />
3 Max Planck Institute for Biochemistry, Dept. Molecular Structural Biology, Am<br />
Klopferspitz 18, 82152 Martinsried, Germany<br />
Keywords: Bacteria, Planctomycetes, Rhodopirellula baltica, expression profiling, whole<br />
genome array, microarray, hypothetical genes, cell cycle, cell compartmentalisation, FM-dye<br />
Author´s e-mail addresses:<br />
P. Wecker: pwecker@mpi-bremen.de<br />
M. Schüler: schueler@biochem.mpg.de<br />
A. Ellrott: aellrott@mpi-bremen.de<br />
F.O. Glöckner: fog@mpi-bremen.de<br />
§ Corresponding author<br />
56
Results and Discussion<br />
Abstract<br />
Background: Rhodopirellula baltica is a marine representative of the globally distributed<br />
phylum Planctomycetes. As typical for Planctomycetes, R. baltica has several unique<br />
properties, like a peptidoglycan-free proteinaceous cell wall, intracellular<br />
compartmentalisation, and it undergoes morphological changes during its life cycle, such as<br />
budding and cell aggregations. The aim of this transcriptomic study was to get first hints on<br />
genes involved in the replication and life cycle of R. baltica which are mostly unknown. Here<br />
we report the results of the first growth dependent expression profiling.<br />
Results: Four different growth phases of a R. baltica culture were analysed using a whole<br />
genome microarray. The number of regulated genes increased from 90 in the exponential to<br />
863 in the late stationary phase when compared to the transition phase. Results indicate a<br />
remodelling of the cell wall and membrane in the exponential phase, sensing of environmental<br />
changes in the transition phase, followed by a stationary-phase specific response. More than<br />
half of the regulated genes per growth stage were annotated as hypothetical proteins. These<br />
genes are probable candidates for functions associated with the planctomycete specific<br />
morphotype-differentiation during the life cycle. Interesting was the growth-dependent<br />
regulation of 12 sulphatases and the up-regulation of genes involved in a predicted C1-<br />
metabolism pathway after 240 h of cultivation.<br />
Conclusion: The whole genome array of R. baltica gave initial insights into the regulation of<br />
all genes during the life cycle. We were able to show activity of genes that might be involved<br />
in the formation of budding and swarmer cells. The transcriptional profiling showed that a<br />
large number of hypothetical proteins function within the cell cycle and in the formation of<br />
the different cell morphologies. The transcriptomic results are contributing to a better<br />
understanding of the so far unknown molecular cell biology underlying the cell<br />
compartmentalisation of R. baltica and, more general, of the phylum Planctomycetales and<br />
possibly even of the PVC superphylum.<br />
57
Results and Discussion<br />
Background<br />
Rhodopirellula baltica SH1 T was isolated from the water column in the Kiel Fjord (Baltic<br />
Sea) and taxonomically grouped within the bacterial phylum Planctomycetes [1, 2]. Members<br />
of this group are abundant in aquatic habitats [3, 4] and considered to play a significant role in<br />
carbon recycling [5]. Planctomycetes share several unique properties, such as peptidoglycanfree<br />
proteinaceous cell walls [6, 7], intracellular compartmentalisation [8] and a mode of<br />
reproduction via budding. The latter results in a life cycle which is characterised by motile<br />
and sessile morphotypes and resembles that of Caulobacter crescentus [9: Gade, 2005 #186].<br />
During early stages of growth, numerous budding mother cells and swarmer cells are present,<br />
as illustrated in FIG 1 (redrawn from Gade et al. [10]). As growth proceeds, the number of<br />
swarmer cells decreases, and single cells form rosettes by attaching to each other. These<br />
aggregates dominate during transition- and stationary phase.<br />
The annotation of the completely sequenced genome of R. baltica allowed an initial<br />
assessment of its genetic potential and revealed several interesting and surprising traits such<br />
as high number of sulphatase genes or a conspicuous C1-metabolism pathway [5 and<br />
references therein]. However, not much information could be inferred from this genome<br />
content survey with respect to genes functioning in the development of the different<br />
morphologies during the cell cycle and its regulation. In general, the cell division machinery<br />
of Bacteria consists of different cell division genes, like the filamentation temperature<br />
sensitive genes (fts) named ftsZ, ftsA, ftsI, ftsL, ftsQ, ftsN, ftsW, ftsK, and the FtsZ interacting<br />
proteinA (zipA) [11]. The ftsZ-gene is the major constituent of the septal replication ring.<br />
Surprisingly, all of these genes, except ftsK, are absent from the genome of R. baltica as well<br />
as from the genomes of other Planctomycetes such as Gemmata obscuriglobus UQM 2246 T ,<br />
Planctomycetes maris DSM 8797 T , and Blastopirellula marina DSM 3645 T , ‘Candidatus<br />
Kuenenia stuttgartiensis’ harbours genes coding for ftsI and ftsW in addition to the ftsK gene.<br />
Teeling [12] concluded that the cell division in Planctomycetes, like in Chlamydia, must take<br />
place by a totally unknown mechanism.<br />
Regarding the morphological changes, the life cycle of R. baltica seems to be similar to<br />
Caulobacter crescentus CB15. In C. crescentus, the key gene of the cell-cycle is the control<br />
protein CtrA. It acts as the master regulator by overseeing several important cellular processes<br />
[13]. Within the R. baltica genome no homologue to the protein CtrA has been found.<br />
However, some base patterns (e.g. TTAAN 7 AAAC), were found which are similar to CtrA<br />
58
Results and Discussion<br />
and CzcR – binding site patterns (TTAAN 7 TTAA). CzcR is a control gene and responsible for<br />
induction of resistance.<br />
It was anticipated that genes involved in the cellular changes during the life cycle show<br />
growth-dependent changes on the mRNA level. To monitor complex processes such as the<br />
cell cycle, whole genome expression profiling has been shown to be particularly useful [14,<br />
15]. Investigations of the gene expression during the C. crescentus cell cycle revealed that,<br />
surprisingly, around 19% of the genes in the genome are affected [16]. For R. baltica, growthdependent<br />
expression patterns were expected to reveal genes that might be required for<br />
morphotype differentiation and for physiological functions, and cellular processes typical of<br />
this group of organisms. In addition, morphology studies based on fluorescence imaging of<br />
the stained membrane [8] of dividing R. baltica cells were undertaken that to aid in<br />
understanding of how the cell compartments are distributed to the daughter cells during the<br />
unequal cell division.<br />
Results and discussion<br />
Gene expression studies<br />
R. baltica was grown in a defined mineral medium with glucose as carbon source to study<br />
changes on the gene expression level that reflect changes due to different life cycle phases.<br />
Cultures of R. baltica were harvested at the onset of the exponential phase (after 44 h of<br />
incubation), during the exponential phase (62 h), in the transition phase between exponential<br />
and stationary phase (82 h), and in the early and late stationary phase (96 h and 240 h,<br />
respectively). Gade and coworkers [10] have shown that there is a correlation between distinct<br />
morphotypes and different phases of the growth curve of R. baltica cultures based on<br />
microscopic examination. Since the R. baltica cultures used in the present study were not<br />
synchronised, we applied Fluorescence Activated Cell Sorting (FACS) to examine the<br />
morphological distribution in the cultures at each of the time points of interest. Looking at<br />
FIG 3 a correlation can be seen between the scatter plot and the microscopic pictures from<br />
this study with the microscopic observations of Gade et al. [10]. In the early exponential<br />
growth phase the culture was dominated by swarmer and budding cells (FIG. 3 A), in the<br />
transition phase we found single and budding cells as well as rosette formations (FIG. 3 B)<br />
and the stationary phase was dominated by rosette formations (FIG. 3 C).<br />
59
Results and Discussion<br />
Global differential gene expression results<br />
An expression ratio of higher 1.5 or below -1.5 was used as a threshold for significant<br />
changes. The threshold was determined in a self-self-hybridisation experiment.<br />
The total number of genes detected as differentially regulated for each investigated growth<br />
stage is shown in TABLE 1. A complete list of genes can be found in the ADDITIONAL<br />
FILE 1. The comparison of samples taken from the early log-phase (44 h) and the exponential<br />
phase (62 h) showed that 149 genes were regulated, of which 64% are annotated as<br />
hypothetical proteins. The comparison between exponential (62 h) and transition phase (82 h)<br />
resulted in only 90 regulated genes with more than 50% having an unknown function. The<br />
first pronounced change of the transcriptome with 235 regulated genes (63% hypothetical<br />
proteins) was observed when comparing the transition phase (82 h) with the stationary phase<br />
(96 h). A comparison of cultures from 82 h with the late stationary phase (240 h) revealed the<br />
most pronounced changes of the transcriptome in our study: 863 genes were differentially<br />
expressed, of which 61% are annotated as hypothetical proteins (FIG 4).<br />
The assignment of the differentially expressed genes to functional classes according to COG<br />
delivered first hints about the activity in the R. baltica cells at the different growth stages<br />
(TABLE 2). The comparison of the samples from within the exponential phase (44 h and 82 h<br />
with 62 h) showed that a maximum fraction of 2% of the total number of genes was regulated.<br />
Gade et al. [10] observed the same effect on the proteome level and proposed that it might<br />
reflect the favourable nutritional conditions encountered throughout these growth stages.<br />
Genes associated with the metabolism of amino acids [E] and carbohydrates [G], genes for the<br />
energy production and conservation [C], as well as genes for the DNA replication and<br />
recombination [L] were up-regulated in the early exponential phase (44 h) compared to 62 h.<br />
The induced oxidases (RB4416 and RB5701), peptidases (RB4269 and RB6443), lipases and<br />
esterases (RB7072 and RB3413) reflect a higher metabolic activity in the cells.<br />
Interestingly, genes for the “cell envelope biogenesis, outer membrane” [M] and “cell motility<br />
and secretion” [N] were induced at 44 h. Genes coding for flagellin A (flaA - RB4454), for<br />
bacterial type II and III secretion system protein (RB5174) and for diverse transferases<br />
(RB6305 and RB6624) were induced. Thus, it seems that at the onset of the exponential phase<br />
(44 h) compared to the mid-exponential phase (62 h) a remodelling of the cell wall and the<br />
morphology was ongoing, perhaps triggered by an enhancement of the swarmer cell<br />
production which is likely very costly in energy and would explain the observed higher<br />
expression level of genes from COG category C [17]. Although R. baltica was in the middle<br />
60
Results and Discussion<br />
of the exponential phase (62 h), fewer genes are regulated than in the stationary phase. This<br />
indicates that all genes necessary for exponential growth under favourable conditions were<br />
already expressed in early log phase. R. baltica increased the glutamate dehydrogenase<br />
(RB6930) level. This enzyme is involved in the biosynthesis of arginine, glutamate and<br />
proline. Latter is one of the major components of the cell wall of R. baltica.<br />
The cell density has reached the highest point in the R. baltica flask culture at the transition<br />
phase. Cells seem stressed as genes coding for glutathione peroxidase (RB2244), thioredoxin<br />
(RB121260) and bacterioferritin comigratory protein (RB12362) were up-regulated, which<br />
corresponds to proteomic data [10]. General stress protectants like trehalose synthase<br />
(RB5196), universal stress protein (uspE - RB4742) and chaperones (e.g. RB8966) were upregulated.<br />
The observation that diverse dehydrogenasese, hydrolases and reductases were<br />
regulated, suggests a metabolic adaptation of R. baltica to the nutrient limitation in the<br />
culture. The transition from the logarithmic phase of growth into the stationary phase is not a<br />
passive process; and the expression levels of a number of gene products underwent substantial<br />
changes during this period [18]. The transition phase is usually characterised by the onset of<br />
nutrient depletion and starvation stress; cells need to prepare for long-term survival under<br />
suboptimal or even unfavourable conditions. Earlier experiments have shown that R. baltica<br />
survives for at least 14 days in the stationary phase at 28°C [10]. Apparently, R. baltica is<br />
well adapted for survival under nutrient limiting conditions which are often encountered in its<br />
natural environment. We compared expression profiles of the transition phase (82 h) with that<br />
of the early (96 h) and the late stationary phase (240 h). The intersection of both sample sets<br />
(96 h vs. 82 h and 240 h vs. 82 h), visualised by a Venn diagram (FIG 5), revealed 103 genes<br />
with similar regulation behaviour. 71 out of the 103 genes are annotated as hypothetical<br />
proteins and most of the remaining 32 genes are stress-related as expected. The general<br />
response of cells to the conditions in stationary phase consists of the induction of genes<br />
associated with energy production, amino acid biosynthesis, signal transduction,<br />
transcriptional regulation, stress response and protein folding. Genes coding for enzymes of<br />
the carbon metabolism, translation control, energy production and amino acid biosynthesis<br />
were repressed.<br />
R. baltica decreased the expression level of genes belonging to the ribosomal machinery<br />
(RB12197, RB2543, RB264, RB5801, RB5804, RB7022, RB7818, RB8253, RB8725 and<br />
RB9304), to transcription regulation (RB10339, RB10458, RB11223, RB11242, RB12372<br />
and RB1392) and to DNA replication and recombination (RB11863 and RB3281) in the late<br />
stationary phase, suggesting a rearrangement of the genome. In addition, the organism<br />
61
Results and Discussion<br />
expressed many genes coding for transposases, integrases and recombinases (RB10096,<br />
RB11303, RB11750, RB1190, RB3144, RB4826, RB5887, RB7388, RB12239, RB2186,<br />
RB6736, RB9907, RB6167, RB7389 and RB934), suggesting that under stress conditions<br />
genome arrangements take place. Six out of those 15 genes (RB11750, RB12239, RB2186,<br />
RB9907, RB7389, and RB934) have been found active under abiotic stress factors, like<br />
temperature and salinity ([19] in prep), which indicates that expression of these genes<br />
constitutes a response to general stress. Noteworthy is the up-regulation of genes for the<br />
phenylalanine, tyrosine and tryptophane biosynthesis (RB6822 and RB6147). The latter have<br />
been found expressed on the proteome level as well [10] but the physiological meaning is<br />
presently unknown. Also genes involved in the biosynthesis of serine, threonine and glycine<br />
(RB6932) and of lysine (RB4382) were up-regulated. Interesting is the induction of genes for<br />
the ubiquinone biosynthesis (RB2748, RB2749 and RB2750). Because it is known from<br />
A. tumefaciens and R. sphaeroides, that when oxygen is lacking, the production of ubiquinone<br />
increases [20]. In addition, it seems that the composition of the cell wall responded to changes<br />
in the physiological state of the R. baltica cell during the late stationary phase. The organism<br />
exported more polysaccharides. And diverse genes belonging to the cell membrane class [M]<br />
were up-regulated. R. baltica activated the production of many outer membrane transporter<br />
and biopolymer exporters (RB12053, RB12055, RB9308, RB1120, RB754, RB12279 and<br />
RB10255), as well as several transferases (RB12690, RB2507, RB2985 and RB5846) for<br />
alteration of the lipopolysaccharides that belong to the cell membrane. In addition, we found<br />
19 genes annotated as membrane proteins regulated, as well as 13 secreted proteins. Two<br />
genes coding for bacterial micro-compartment proteins (RB2585 and RB2586) were upregulated.<br />
They are supposed to form primitive organelles in a variety of bacteria [21].<br />
Perhaps the cell wall became adapted to maintain the cell shape, cell division and genome<br />
segregation and/or is acting as an intermediary between the cell and its environment. It could<br />
be that after entering the stationary phase, R. baltica cells, like the marine bacterium<br />
’Candidatus Pelagibacter ubique‘, undergo morphological changes (cell shape), enabling<br />
them to return to exponential growth again after being transferred to fresh medium or to a<br />
nutrient rich environment respectively [22]. We also found hints as to the formation of the<br />
storage compound glycogen in the expression profile of the stationary phase. So far glgB<br />
(RB548) was only found to be expressed when R. baltica cultures were grown on agar plate<br />
[10]. In the present study, we found two genes induced; glgB (RB548) at 96 h and glgX<br />
(RB4894) at 240 h.<br />
62
Results and Discussion<br />
In the following sections, regulation of selected genes will be described and discussed<br />
according to their functional classification.<br />
Sulphatases<br />
The genome of R. baltica is harbouring 110 sulphatases. It has been proposed on the basis of<br />
their sequence similarity that they are involved in the utilisation of carbon from complex<br />
sulphated heteropolysaccharides [5].<br />
During transcriptional analysis of the different R. baltica growth stages we found 12<br />
sulphatases up- or down-regulated including four putative choline sulphatases (RB10161,<br />
RB110, RB2254 and RB5282), six different arylsulphatases (RB13148, RB4815, RB5195,<br />
RB684, RB686 and RB7875) and two other sulphatases (RB5294 and RB5424) (TABLE 3).<br />
Up-regulation of sulphatase genes cannot have been caused by substrate induction, since the<br />
mineral medium used in this study did not contain any sulphated carbon compounds. Five of<br />
the sulphatase genes mentioned above (RB110, RB2254, RB4815, RB5294 and RB686) were<br />
also found up-regulated when the expression profile of R. baltica cells grown on a solid<br />
surface was compared with that of cells grown in liquid culture (data not shown). The<br />
observation that certain sulphatases were only expressed in specific growth phases suggests<br />
that degrading polymers is not the only role of sulphatase genes in R. baltica. Based on the<br />
differential expression pattern of these sulphatase genes we propose an involvement in<br />
structural remodelling during the morphological differentiation of R. baltica cells, such as the<br />
formation of the holdfast substance, which is needed to attach to surfaces, the production of<br />
slime polysaccharides [23], or in the cell wall formation, which consists of a protein sacculus<br />
with disulphide bonds [7]. More experimental work is needed to clarify the potentially diverse<br />
role of the many sulphatase genes in R. baltica.<br />
Lipids<br />
In contrast to Gade et al. [10], who found indications that the biosynthesis of sulphated<br />
saturated fatty acids occurs specifically during the stationary phase, we only found that delta 9<br />
acyl-lipid fatty acid desaturase (RB4876), belonging to the unsaturated fatty acid pathway,<br />
was up-regulated in the transition phase (82 h). Furthermore, malonyl CoA-acyl carrier<br />
protein transacylase (RB314), belonging to the fatty acid pathway, was up-regulated in the<br />
late stationary phase (240 h). Fatty acids appear also in the membrane of diverse groups of<br />
Planctomycetes [24]. RB314 together with RB318 was found up-regulated under stress<br />
63
Results and Discussion<br />
conditions [19], leading to the assumption of a general stress response to enhance the stability<br />
of the membrane to protect the cell.<br />
C1-metabolism<br />
R. baltica has the genetic potential to degrade C1-compounds and the functional prediction<br />
revealed all enzymes necessary for the oxidation of formaldehyde to formate [5]. Out of the<br />
13 proposed genes encoding enzymes of C1-metabolism [25] four genes were regulated in our<br />
experiment. The following genes were induced in the late stationary phase: fae (RB9338),<br />
mtdA (EC 1.5.1.5 - RB10295) and citG (Orf7 - RB10246), whereas ATP-dependent<br />
carboligase (OrfY -RB10299) was found to be induced in the exponential and repressed in the<br />
early stationary phase. Although in silico studies have revealed the presence of this C1-<br />
pathway also in other Planctomycetes [26, 27] the specific functions of the genes in<br />
Planctomycetes are still unknown. The expression of some of the genes observed in this study<br />
suggests at least that this pathway has physiological relevance in R. baltica and probably in<br />
other Planctomycetes.<br />
Cell division and cell cycle<br />
Not much is known about the regulation of the replication and the process of cell division in<br />
Planctomycetes. None of the 20 R. baltica genes assigned to the COG category “cell division<br />
and chromosome partitioning” [D] were found to be regulated during this study, nor any of<br />
the homologues of genes that are generally known to be involved in the cell division, but<br />
without specific category assignment (ADDITIONAL FILE 2). This suggests that a number<br />
of so far hypothetical R. baltica proteins must play a role in the regulation of replication and<br />
cell divison. R. baltica exhibits a life cycle similar to C. crescentus. DnaK/DnaJ and<br />
GroES/GroEL are the best-characterised chaperone systems in bacteria and induced in cell<br />
cycle regulation and under heat shock in C. crescentus [28]. Those chaperones have an<br />
important role in chromosome replication and partition and/or cell division. The R. baltica<br />
genome harbours genes coding for groEL (RB10629 and RB8970), dnaK (RB5754 and<br />
RB5755) and dnaJ (RB8972) and groES (RB) in the genome. However, only the expression<br />
of groEL (RB10629) is up-regulated at 82 h in the life cycle experiment, which could also be<br />
a general response to stress [19].<br />
R. baltica also possesses genes encoding dnaA (RB11579 and RB1706) which initiates not<br />
only the DNA replication but also promotes expression of the components necessary for<br />
64
Results and Discussion<br />
successful chromosome duplication in C. crescentus [28]. However, dnaA was not found to be<br />
regulated during our experiment.<br />
Alternative sigma factors and two-component regulators<br />
The R. baltica genome is harbouring 33 genes coding for RNA polymerase sigma factors with<br />
extra cytoplasmic function (ECF). 5 out of 33 genes were found to be regulated in this study.<br />
ECF-sigma factors are known to be involved in the regulation of bacterial interaction with the<br />
extracellular environment, e.g. sensing of stationary-phase conditions and initiation of the<br />
global response. This should lead to an increased survivability of R. baltica [22].<br />
Furthermore, it is known that ECF sigma factors are involved in the cell envelope response of<br />
B. licheniformis [29] and in the cell division of B. subtilis [30] The gene coding RNA<br />
polymerase sigma-70 factor ECF (RB11242) was induced in the transition phase (82 h) and<br />
repressed together with three other genes coding ECF-sigma factors (RB12372, RB138 and<br />
RB8027) in the stationary phase. RB9852 was up-regulated in the early stationary phase.<br />
In addition, two sigma factors were regulated in the late stationary phase; RB7924 was upand<br />
RB1392 down-regulated. RB1392 could be responsible for the regulation of the cell<br />
division inhibitor (RB1391). A sigma-54 dependent regulator (RB7579) was also induced.<br />
Two out of four genes coding for the RNA polymerase sigma factor rpoD (RB10339 and<br />
RB6780) were down-regulated. They are also found to be reduced in expression during the S-<br />
phase of C. crecsentus [14]. The authors of this study suggest a transcriptional regulation of<br />
the central metabolism. Although R. baltica possesses the stationary-phase sigma factor rpoS<br />
(RB3727), which is known to lead to a global stationary-phase response, it was not regulated<br />
in our experiment.<br />
Two-component-system response regulators allow bacteria to respond to changes in the<br />
environment. 68 genes in the R. baltica genome were annotated as response regulators, of<br />
which four were found to be differentially expressed in this study (RB4484, RB10308,<br />
RB10491 and RB6403). Response regulators RB10308 and RB4487 were detected in the<br />
stationary phase proteome [10]. In our case, RB4487 was induced at 96 h and repressed at<br />
240 h. The response regulator RB6403 was also repressed in the late stationary phase, which<br />
was as well shown for the proteome [10]. The gene coding for the response regulator<br />
(RB10491) was not found to be differentially expressed on the transcriptome level, but it was<br />
induced in the stationary phase on the proteome level [10]. It seems that these regulator<br />
proteins are involved in the stress response of R. baltica during stationary phase conditions.<br />
65
Results and Discussion<br />
Taken, for example, the two stress sensor genes encoding carbon storage regulator (CrsA -<br />
RB10261 and RB8149); only RB10261 was induced at 96 h but both were repressed in the<br />
late stationary phase (240h). Noteworthy is the fact that no histidine kinase was found to be<br />
differentially expressed during our experiment, whereas sixteen histidine kinases are found to<br />
be expressed in a cell-cycle-dependent manner in C. crescentus [14]. Those transmembrane<br />
sensor proteins plus response regulator are supposed to be involved in the monitoring of<br />
environmental conditions and respond to perturbations harmful to the cell envelope [29].<br />
Hypothetical proteins<br />
It was not surprising that a large number of genes found to be differentially expressed in this<br />
study encoded (conserved) hypothetical proteins, given the limited functional knowledge on<br />
cellular processes in Planctomycetes. Some of these regulated hypothetical proteins were<br />
planctomycete-specific. They are supposed to code for the unique cellular planctomycetal<br />
characteristics or for specific metabolic traits, or are proposed to be involved in regulation<br />
[26]. Among the regulated hypothetical proteins 51 ORFs with a Domain of Unknown<br />
Function (DUF) were found. The R. baltica genome encodes 74 planctomycetes-groupspecific<br />
proteins that are annotated as hypothetical proteins containing DUF1559 (PF07596)<br />
and comprise a new family of “solute-binding” proteins. These are high affinity transport<br />
systems which could have an additional function in signal transduction systems [31]. Ten of<br />
these proteins were found to be expressed, in this study, and of these nine were especially upregulated<br />
in the stationary phase. RB2556 and RB4325 encoding for proteins of the family<br />
“planctomycete PGAMP” (PF07644) and RB560, coding for a protein containing the DGPF<br />
domain (PF04946) were up-regulated in the late stationary phase (240 h). Their function is<br />
unknown and so far no further data are available to support our speculation that they are in<br />
involved in morphological changes in Planctomycetes. In proteome studies [10], the products<br />
of 14 genes annotated as hypothetical proteins are assumed to be involved in the cell cycle<br />
and/or morphotype differentiation rather than stress or nutrient limitation response. In part,<br />
the results of the present transcriptome analysis support the data gained from an earlier<br />
proteome study [10]. The authors of the latter propose for the three planctomycete-specific<br />
proteins (RB5240, RB6221 and RB6941) an involvement in proliferation or swarmer cell<br />
differentiation. On the transcriptome level RB6221 and RB6941 were down-regulated in the<br />
late stationary phase. RB5240 was not differentially expressed at all. None of the three were<br />
regulated under stress [19]. Gade et al. [10] also propose that the five unique proteins<br />
66
Results and Discussion<br />
RB3577, RB5268, RB7235, RB8559 and RB12461, whose expression has been found to be<br />
increased in the stationary phase, might be involved in the rosette formation or surface<br />
attachment. Neither of them were found to be regulated in our growth experiments. However,<br />
since RB7235 was induced 20 min after cold shock and 10 min after salt shock and RB12461<br />
was induced 20 min after heat shock [19] and at least additional role in stress response should<br />
be considered for these genes (ADDITIONAL FILE 3).<br />
Cluster-overview<br />
In order to get some more information on the potential function of those differentially<br />
expressed genes that encode hypothetical proteins we delineated groups of genes with similar<br />
expression pattern during culture growth. Information on the genes populating each cluster<br />
can be found in the ADDITIONAL FILE 4. TABLE 5 shows the average expression pattern<br />
of the clusters that are discussed in the following. Cluster 4, 12 and 17 group genes that were<br />
only regulated in the exponential phase where nutrient limitation or stress conditions are<br />
unlikely. Therefore, these genes can be supposed to have a function in the morphology<br />
differentiation to swarmer cells or in the budding process. Genes belonging to cluster 11, 13,<br />
19, 20, 21, 22 and 23 were up-regulated during the transition phase. Cluster 21 is interesting,<br />
with genes induced only in the transition phase (82 h) and repressed at 240h. They might be<br />
involved in stress monitoring and handling. Genes annotated as hypothetical proteins<br />
belonging to the early stationary phase (96 h) were grouped in cluster 18, 24 and 25. Cluster<br />
18 and 24 consist of hypothetical proteins that might be involved in the morphology changes,<br />
e.g. cell shape and wall. Cluster 25 consists mainly of genes belonging to the energy<br />
production plus hypothetical proteins. Genes only induced in the late stationary phase (240 h)<br />
belong to the cluster 3 and 16. Finally, cluster 14 genes were up-regulated in the exponential<br />
(62 h) and down-regulated in the stationary (96 h) phase in comparison to the transition phase.<br />
This cluster contains one sulphatase gene and 14 genes encoding hypothetical proteins. It can<br />
be assumed that these have a function in the morphotype differentiation.<br />
Cell biology studies<br />
The whole genome array for the marine planctomycete R. baltica [19] was taken as a tool to<br />
explore the regulation of all genes during the life cycle. The expression data provided hints as<br />
to which genes might be involved in the cell division of R. baltica. In order to get a better<br />
understanding of how the division process of the compartments takes place in three-<br />
67
Results and Discussion<br />
dimensional space, for instance how the budding mechanism works, which membranes break<br />
down in the course of cell division or how the nucleoid is segregated to daughter cells, we<br />
conducted cell biology experiments using specific fluorescent DNA- and membrane-markers<br />
on cells from the transition phase of the R. baltica culture. Fluorescence membrane staining<br />
and 3D-reconstruction using cryosections has previously been done on the planctomycete<br />
Gemmata obscuriglobus [8, 32] to understand more about the cell compartmentalisation. We<br />
stained the R. baltica cells with two different permanent membrane dyes, a cytoplasmic<br />
membrane specific dye (FM4-64), and a general endomembrane dye (DiOC 6 (3)). The DNAspecific<br />
fluorescent dye DAPI was used for the nucleoid. Results are shown in FIG 6 and<br />
FIG 7 A, B and C. FIG 7 shows dividing and sessile R. baltica cells stained with DAPI and A)<br />
DiOC 6 (3) or B) FM4-64. FIG 7 C) shows a composite picture of R. baltica cells forming<br />
rosettes stained with all three dyes (DAPI, DiOC 6 (3) andFM4-64).<br />
To get a different resolution on the whole single cell level we applied the new nanoSIMStechnology,<br />
which is available at the <strong>University</strong> of Mainz, to membrane-stained R. baltica<br />
cells from the transition phase of the culture. Both membrane dyes contain halogen atoms<br />
(FM4-64 bromine, DiOC 6 (3) iodine). The incorporated membrane dye halogens can be<br />
detected and quantified by the nanoSIMS microprobe and displayed by a graphical<br />
reconstruction. Initial results show that the method is successful. The shape of the R. baltica<br />
cell was recognisable (FIG 8). The background was satisfying and, despite the comparative<br />
large cell size, the first pictures look promising and encourage further studies.<br />
68
Results and Discussion<br />
Conclusions<br />
Our global transcriptome analysis, for example, documents the morphological changes during<br />
the exponential growth. The lack of nutrients and a high cell density in the stationary phase<br />
imposes the activation of general stress response genes, genes for the reorganisation of the<br />
genome and the cell wall. The ability to sense and adapt to a changing environment can be<br />
monitored by the up-regulation of ECF-sigma factors and response regulators. The results<br />
provide also hints that a subset of the 110 sulphatases might be involved in the formation of<br />
planctomycete-specific morphological features (e.g. cell wall components or holdfast<br />
substance), beyond sulphated carbon compound utilisation.<br />
Proteins of unknown function constitute a major part of the regulated genes in all growth<br />
stages of R. baltica. Their correlation to morphotype and their expression only at specific<br />
growth stages provides first hints about their function in the life cycle and the underlying<br />
cellular processes in R. baltica. Follow-up nanoSIMS studies will help to understand how the<br />
division process of the compartments in three-dimensional space is taking place. Our results<br />
form the basis for further studies on the transcriptome level to reveal the unknown mechanism<br />
behind the cell cycle and cell division of R. baltica, as a representative of the phylum<br />
Planctomycetes.<br />
Material and Methods<br />
Cultivation of Rhodopirellula baltica cells<br />
Cells of R. baltica were grown in batch cultures (500ml) with mineral medium containing<br />
10 mM glucose as sole carbon and 1 mM ammonium chloride as nitrogen source [33]. The<br />
R. baltica cultures were incubated at 28°C on a rotary shaker. Three independent batch<br />
cultures (biological replicates) per incubation time point were inoculated, totalling 18 flasks at<br />
the beginning of the experiment.<br />
Harvesting of cells and preparation for microarray hybridisations<br />
Cultures were harvested by centrifugation (Beckman Coulter TM Avanti TM J-20XP, JA10<br />
Rotor, 20 min, 6000 rpm, 4°C), after different incubation times (44, 68, 82, 96 and 240 h).<br />
The culture broth was collected in 500 ml tubes and swirled briefly in an ethanol/dry ice bath<br />
to rapidly cool down the cultures and "freeze" the RNA profile. Subsequently, the broth was<br />
69
Results and Discussion<br />
centrifuged at 6000 rpm for 20 min at 4°C (Beckman Coulter TM Avanti TM J-20XP, JA10<br />
Rotor). The pellets were re-suspended in 0.1 M Tris-HCl and then re-centrifuged to obtain<br />
cell pellets that were shock-frozen in liquid nitrogen and stored at -80°C. Total RNA<br />
isolation, cDNA synthesis, array construction, hybridisation and washing were performed as<br />
previously described [19]. A detailed description of the array can be found at the NCBI´s<br />
Gene Expression Omnibus (GEO) database [34] under accession number GPL7654 and [19].<br />
Experimental design and sample preparation<br />
For expression profiling and microscopic analysis samples were collected at 44, 62, 82, 96<br />
and 240 h. In two independent hybridisations for each sample, cDNA samples were<br />
hybridised according to FIG 2. At each growth time point the sample was labelled either with<br />
Alexa 647 or Alexa 546 for the array analysis. The data shown are based on the analysis of<br />
the two replicates performed for each of the conditions. Dots and arrowheads represent<br />
samples labelled with Alexa 546 and Alexa 647, respectively.<br />
Signal detection and data analysis<br />
Slides were scanned at a resolution of 5 µm using a Scan<strong>Array</strong> Express Microarray scanner<br />
(Perkin Elmer). The image analysis software provided with this scanner was used for<br />
automatic spot detection and signal quantification. Raw data were automatically processed<br />
using the microarray data analysis software tool MADA [35]. LOWESS Normalisation was<br />
applied on an R-versus-I plot with a smoothing factor of 0.5. If replicates were available, the<br />
expression data (ratio) of all hybridisations were averaged and combined to one expression<br />
data point (ratio).<br />
Cluster analysis and Database<br />
Differentially expressed genes displaying the complete loop profile were clustered using the<br />
k-means clustering approach (Euclidean distance metric, k = 25 clusters and 9 iterations (max.<br />
500)) [36] with the software tool Multi-experiment Viewer MeV Version 4.0.2 from the TM4<br />
microarray software suite [37]. The genome of Rhodopirellula baltica was automatically reannotated<br />
based on updated homology searches (June 2005 - MicHanThi [38]). The updated<br />
annotation including all tool results are publicly available [39]. JCoast [40] was used as a tool<br />
for the visualisation, interpretation, statistic (COG-assignment) and comparison of genomic<br />
data stored in GenDB V2.2 [41]. Venn diagrams were generated by BioVenn [42].<br />
70
Results and Discussion<br />
A detailed description of the life cycle data will be made publicly available at the GEO<br />
database.<br />
Fluorescence microscopy modified after [8]<br />
R. baltica cells were harvested into Milli-Q-grade deionised water. After centrifuging, the<br />
pellet was re-suspended in dye solution for 5 to 60 min before pipetting 5 µl onto an agarose<br />
slab on a glass microscope slide, allowed to soak in and a coverslip was placed on top. The<br />
solution of dyes used contained final concentrations of 3.3 μg ml -1 – DiOC 6 (3) (3,3′-<br />
dihexyloxacarbocyanine iodide) (Molecular Probes), 200 μg ml -1 – DAPI (4′-6-diamidino-2-<br />
phenylindole) (Sigma) and 3.3 μg ml -1 – FM4–64 (N- (3 triethylammoniumpropyl)-4-(pdiethylaminophenyl-hexatrienyl)<br />
pyridimium dibromide) (Molecular Probes) or 3.3 μg ml -1 –<br />
FM4–64FX (molecular probes) [43]. Specimens were viewed using a Zeiss Axioskop2<br />
motplus epifluorescence microscope (Carl Zeiss, Jena, Germany) using a ×100 objective in<br />
conjunction with AxioVision software and a AxioCam MRm digital camera. Images were<br />
acquired separately for each excitation-emission filter block combination appropriate to each<br />
dye used (filter set for 365 nm excitation of DAPI, filter set for 450–490 nm excitation of<br />
DiOC 6 (3) and filter set for 546 nm excitation of FM4–64). The resulting image files were<br />
merged for final image production including brightness and contrast adjustment using the<br />
indicated software.<br />
Nano-Scale secondary-ion mass spectrometry (nanoSIMS)<br />
A 1 ml mix containing dye-stock-solution plus R. baltica cells were incubated for 30 min.<br />
R. baltica cells plus a) DiOC 6 (3); b) FM4–64; c) DiOC 6 (3) and FM4–64; were analysed. 5 µl<br />
aliquot of each sample was taken for microscopic method control. Stained cells were collected<br />
onto a gold-palladium (20:80) coated filter and washed with 20 ml Milli-Q-grade deionised<br />
water. Samples were stored in the darkness and at room temperature until analysis. The filters<br />
were analysed using a nanoSIMS 50 manufactured by CAMECA [44] at the <strong>University</strong> of<br />
Mainz, Germany. Secondary ion images of the halogens bromide and iodine were recorded<br />
simultaneously using 5 electron multipliers. Images and data were processed using the<br />
proprietary CAMECA WinImage processing software.<br />
71
Results and Discussion<br />
Flow cytometry analysis modified after [45]<br />
Samples used for flow cytometry and for the microscopy were taken from the same batch<br />
cultures of R. baltica at different growth stages: early exponential (OD 600 0.3), exponential<br />
(OD 600 0.5) and stationary phase (OD 600 0.8). For flow cytometry 1%-PFA-fixed R. baltica<br />
cells, stained with SYBR Green I DNA dye, were flow-sorted using a FACSort instrument<br />
(Becton Dickinson) with standard laser and detection conFIGuration. Yellow-green 0.5 µm<br />
bead standards (Fluoresbrite Microparticles) were used in all analyses as an internal standard.<br />
Triplicates per sample were measured 1 min and displayed as a dotplot diagramm<br />
(CellQuest), which is representing DNA-SYBR Green I fluorescence versus 90° light scatter<br />
(SSC). The dotplot diagrams were used for defining gates. 5 gates (regions) were selected: R1<br />
(beads), R2 (swarmer, single cells), R3 (budding cells), R4 (rosette formations), R5 (total cell<br />
count). The assignment of the gates R2 to R4 arose from the corresponding microscopy<br />
pictures.<br />
Competing interests<br />
The authors declare that they have no competing interests<br />
Author’s contribution<br />
PW conceived the study, initiated and conducted the experimental analysis, validated<br />
microarray and optimised experimental steps, wrote the manuscript, did the statistical analysis<br />
and analysed the data.<br />
MS contributed background information and was involved in the writing and finishing of the<br />
manuscript.<br />
AE wrote MADA the microarray analysis tool and designed the microarrays.<br />
FOG contributed background information and was involved in the writing of the manuscript.<br />
All authors read and approved the final manuscript.<br />
Acknowledgements<br />
We would like to Bernhard Fuchs for the introduction of FACS-technology and Sebastian<br />
Rokitta for excellent technical assistance. We also thank Niculina Musat for the introduction<br />
into nanoSIMS-technology and her for the attendance to take the samples for analysing to the<br />
<strong>University</strong> of Mainz.<br />
72
Results and Discussion<br />
The setup of the microarray and transcriptomic analysis of Rhodopirellula baltica was<br />
supported by the European Commission contract GOCE-CT-2004-505403.<br />
Abbreviations<br />
COG Cluster of Orthologous Group<br />
RB Rhodopirellula baltica<br />
R. Rhodopirellula<br />
GEO Gene Expression Omnibus<br />
ORF Open Reading Frame<br />
ECF Extra cytoplasmic Function<br />
Fts filamentation temperature sensitive<br />
FACS Fluorescence Activated Cell Sorting<br />
73
Results and Discussion<br />
Reference<br />
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23. Kumar A: Bacterial exopolysaccharides - a perception. J Bas Microbiol 2007, 47:103-117.<br />
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transfer enzymes in Planctomycetes: Phylogenetic implications of their unexpected presence in<br />
this phylum. J Mol Evol 2004, 59:571-586.<br />
26. Woebken D, Teeling H, Wecker P, Dumitriu A, Kostadinov I, DeLong EF, Amann R, Glöckner FO:<br />
Fosmids of novel marine Planctomycetes from the Namibian and Oregon coast upwelling systems<br />
and their cross-comparison with planctomycete genomes. ISME Journal 2007, 1(5):419-435.<br />
27. Chistoserdova L, Jenkins C, Kalyuzhnaya M, Marx C, Lapidus A, Vorholt J: The enigmatic<br />
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33. Rabus R, Gade D, Helbig R, Bauer M, Glöckner FO, Kube M, Schlesner H, Reinhardt R, Amann R:<br />
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adipose tissue. Genes Dev 2000, 14(8):963-980.<br />
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TZI-Bremen; 2007.<br />
39. The reannotated Rhodopirellula baltica genome [http://gendb.mpi-bremen.de/gendb/BX119912]<br />
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biologist-centric software tool for data mining and comparison of prokaryotic (meta)genomes.<br />
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Nucl Acids Res 2003, 31(8):2187-2195.<br />
42. Hulsen T, de Vlieg J, Alkema W: BioVenn - a web application for the comparison and visualization<br />
of biological lists using area-proportional Venn diagrams. BMC Genomics 2008, 9(1):488.<br />
43. Bolte S, Talbot C, Boutte Y, Catrice O, Read ND, Satiat-Jeunemaitre B: FM-dyes as experimental<br />
probes for dissecting vesicle trafficking in living plant cells. Journal of Microscopy 2004, 214:159-<br />
173.<br />
44. Musat N, Halm H, Winterholler B, Hoppe P, Peduzzi S, Hillion F, Horreard F, Amann R, Jörgensen<br />
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In., vol. 105; 2008: 17861-17866.<br />
45. Mary I, Heywood JL, Fuchs BM, Amann R, Tarran GA, Burkill PH, Zubkov MV: SAR11 dominace<br />
among metabolically active low nucleic acid bacterioplankton in surface waters along the<br />
Atlantica meridional transcet. Aquatic Microbial Ecology 2006, 45:107-113.<br />
46. Fuerst AJ: Intracellular compartmentation in Planctomycetes. Annu Rev Microbiol 2005, 59:299-<br />
328.<br />
47. Gade D, Theiss D, Lange D, Mirgorodskaya E, Lombardot T, Glöckner FO, Kube M, Reinhardt R,<br />
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Mapping the soluble proteins. Proteomics 2005, 5(14):3654-3671.<br />
75
Results and Discussion<br />
48. Hieu CX, Voigt B, Albrecht D, Becher D, Lombardot T, Glöckner FO, Amann R, Hecker M, Schweder<br />
T: Detailed proteome analysis of growing cells of the planctomycete Rhodopirellula baltica SH1 T .<br />
Proteomics 2008, 8(8):1608-1623.<br />
FIGure legends<br />
FIG 1 Schematic drawing of the life cycle of R. baltica. See text for details [10].<br />
44 h 62 h 82 h<br />
240 h<br />
92 h<br />
FIG 2 Hybridisation scheme R. baltica cultures were harvested at five time points. cDNA samples were<br />
hybridised in two replicates. Dots and arrowheads represent Alexa 546- and Alexa 647-labelled samples,<br />
respectively.<br />
76
Results and Discussion<br />
R4<br />
R4<br />
R2<br />
R3<br />
R2<br />
R3<br />
A B C<br />
FIG 3 Microscope pictures of R. baltica cultures taken from different growth phase stages plus matching FACSscatter<br />
plot. A) exponential phase bar 5 µm, B) late exponential phase, C) stationary phase bar 10 µm. R2<br />
(swarmer and single cells), R3 (budding cells) and R4 (rosette formations).<br />
900<br />
800<br />
Number of regulated genes<br />
700<br />
600<br />
500<br />
400<br />
300<br />
200<br />
100<br />
0<br />
322<br />
541<br />
67<br />
110<br />
25<br />
168<br />
39 65<br />
62 h vs. 44 h 82 h vs. 62 h 92 h vs. 82 h 240 h vs. 82 h<br />
induced<br />
repressed<br />
FIG 4 shows the number of significantly up- and down-regulated genes during the life cycle at the four measured<br />
time points. White means up-regulated and grey down-regulated.<br />
77
Results and Discussion<br />
44 h vs. 62 h<br />
132<br />
720<br />
6<br />
4<br />
7<br />
240 h vs. 82 h<br />
210<br />
66<br />
82 h vs. 62 h<br />
14<br />
92 h vs. 82 h<br />
34<br />
6<br />
38<br />
12<br />
82 h vs. 62 h<br />
103<br />
114<br />
92 h vs. 82 h<br />
FIG 5 Venn diagrams showing the distribution of all regulated genes.<br />
Nucleoid<br />
DAPI<br />
Intracytoplasmic membrane<br />
DiOC 6 (3)<br />
Cytoplasmic membrane<br />
FM4-64<br />
Pirellulosome<br />
Paryphoplasma<br />
Cell wall<br />
FIG 6 Diagrams of cell organisation and compartmentalisation in R. baltica modified after Fuerst et al. [46].<br />
DNA-specific fluorescence dye DAPI (blue) stains nucleoid, membrane-specific dye FM4-64 (red) stains the<br />
external cytoplasm membrane and membrane-specific dye DiOC6(3) (green) passes through the external<br />
membrane and stains the internal membranes.<br />
78
Results and Discussion<br />
FIG 7 A) Fluorescence microscopy of individual stained budding and sessile R. baltica cells.<br />
The DAPI-staining nucleoid (blue) can be seen to be surrounded by the internal DiOC 6 (3)-staining membrane of<br />
the ICM (green). (Scale Bar, 3 µm)<br />
79
Results and Discussion<br />
FIG 7 B) Fluorescence microscopy of individual stained budding and sessile R. baltica cells.<br />
Composite picture of DAPI-staining and FM4-64-staining cytoplasmic membrane (red) indicating the outer cell<br />
boundary . (Scale Bar, 2 µm)<br />
80
Results and Discussion<br />
FIG 7 C) Fluorescence microscopy of individual stained R. baltica cells.<br />
Rosette formation of R. baltica cells. Composite picture (Scale Bar, 3 µm) The DAPI-staining nucleoid (blue)<br />
can be seen to be surrounded by the internal DiOC6(3)-staining membrane of the ICM (green). and the outer cell<br />
boundary is indicated by the FM4-64-staining cytoplasmic membrane (red).<br />
81
Results and Discussion<br />
A<br />
B<br />
C<br />
FIG 8 Parallel secondary ion images of R. baltica cells. (Scale bars, 1µm). The abundance of A) 12 C 14 N - shows<br />
the biomass, B) 81 Br - shows the occurrence of the cytoplasmic stain FM4–64 and C) 127 I - shows the occurrence<br />
internal DiOC 6 (3)-staining membrane.<br />
Tables<br />
TABLE 1 shows the total number of regulated genes during each measured time point of the life cycle and the<br />
fraction of the regulated genes annotated as conserved and/or hypothetical.<br />
number of regulated genes 62 h vs. 44 h 82 h vs. 62 h 96 h vs. 82 h 240 h vs. 82 h<br />
total (%)* 149 (2%) 90 (1%) 235 (3%) 863 (12%)<br />
encoding hypothetical proteins (%)** 95 (64%) 49 (54%) 148 (63%) 526 (61%)<br />
(%)* relative to the total number of 7325 ORFs annotated in R. baltica genome<br />
(%)** relative to the total number of regulated genes<br />
82
Results and Discussion<br />
TABLE 2 Number of regulated genes with an assigned COG-category compared to the whole genome functional<br />
classification assignment according to the NCBI database (cut off e-value e-4). Rows: [C] Energy production<br />
and conversion, [D] Cell division and chromosome partitioning, [E] Amino acid transport and metabolism, [F]<br />
Nucleotide transport and metabolism, [G] Carbohydrate transport and metabolism, [H] Coenzyme metabolism,<br />
[I] Lipid metabolism, [J] Translation, ribosomal structure and biogenesis, [K] Transcription, [L] DNA<br />
replication, recombination and repair, [M] Cell envelope biogenesis, outer membrane, [N] Cell motility and<br />
secretion, [O] Posttranslational modification, protein turnover, chaperones, [P] Inorganic ion transport and<br />
metabolism, [Q] Secondary metabolites biosynthesis, transport and catabolism, [R] General function prediction<br />
only, [S] Function unknown, [T] Signal transduction mechanisms.<br />
COG-Class total in genome 62 h vs. 44 h 82 h vs. 62 h 96 h vs. 82 h 240 h vs. 82 h<br />
up down up down up down up down<br />
C 177 0 3 0 1 5 0 10 7<br />
D 20 0 0 0 0 0 0 0 0<br />
E 215 1 4 2 1 1 1 7 7<br />
F 66 0 0 0 0 2 0 1 1<br />
G 177 1 3 2 0 4 0 8 5<br />
H 117 0 1 0 0 1 0 11 0<br />
I 76 0 1 0 0 0 0 1 2<br />
J 148 0 1 0 0 4 0 4 11<br />
K 162 0 1 2 1 3 0 6 11<br />
L 187 0 2 1 0 1 0 13 4<br />
M 146 0 3 1 0 2 0 10 6<br />
N 114 0 3 0 0 1 1 4 1<br />
O 111 0 0 9 0 6 0 3 12<br />
P 236 0 5 1 1 3 1 15 6<br />
Q 153 0 2 2 0 4 0 9 4<br />
R 434 0 9 3 1 13 2 19 11<br />
S 200 1 2 2 0 6 2 12 11<br />
T 211 0 0 3 0 2 0 6 7<br />
83
Results and Discussion<br />
TABLE 3 Differentially expressed sulphatase genes of R. baltica. Remarks: * chemostat wall unpublished data,<br />
** [26].<br />
ID Product AA<br />
Signal<br />
P<br />
Gene<br />
name<br />
EC<br />
62 vs. 44 h<br />
62 vs. 82 h<br />
96 vs. 82 h<br />
240 vs.82 h<br />
Remarks<br />
RB10612 cholinesulphatase<br />
492 0 betC 3.1.6.6 1.9<br />
RB110 cholinesulphatase<br />
582 0 -1.5 Wall*<br />
RB13148 arylsulphatase A 1012 0.2 -1.5 -2.9 1.5<br />
[precursor]<br />
RB2254 choline<br />
541 0 betC 3.1.6.6 1.8 Wall*<br />
sulphatase<br />
RB4815 arylsulphatase 491 1 ARSA 3.1.6.8 1.9 Wall*<br />
precursor<br />
RB5195 arylsulphatase 505 1 Arsb 3.1.6.12 1.5<br />
RB5282 cholinesulphatase<br />
578 0.5 betC 3.1.6.6 -1.7<br />
RB5294 sulphatase 533 0 1.6 Wall*<br />
RB5424 sulphatase 573 0 -1.8<br />
RB684 Arylsulphatase<br />
precursor<br />
653 0.8 atsA 3.1.6.1 -<br />
1.54<br />
RB686 arylsulphatase 549 1 atsA 3.1.6.1 -1.7 Wall*,<br />
Fosmid**<br />
RB7875 Arylsulphatase A<br />
precursor<br />
492 0.97 ARSA 3.1.6.8 2.32<br />
84
Results and Discussion<br />
TABLE 4 Presence or absence of expression or regulation of genes coding for the C1-transfer enzymes in<br />
R. baltica x = expressed on the proteome or transcriptome level.<br />
Locus Tag Gene name Transcriptome Life cycle<br />
Metagenome Proteome<br />
[26] [47]<br />
RB1696 ORF20<br />
RB3936 pabA<br />
RB3938 ORF19<br />
RB3940 mptG<br />
RB5135 ptpS<br />
RB5439 fmdC<br />
RB5685 ORf22<br />
RB6209 ORF9<br />
RB6210 ORf21 x<br />
RB6292 ORF17<br />
RB6300 pabA<br />
RB6753 pabB<br />
RB6756 ORF5<br />
RB6759 mch x<br />
RB8806 ptpS<br />
RB9338 fae 1.51 x x<br />
RB9834 fm(w)dA x<br />
RB9835 ftr<br />
RB9836 fm(w)dC x<br />
RB10246 ORF7 2.58 x<br />
RB10295 mtdC 1.7 x x<br />
RB10297 fae<br />
RB10299 ORFY 1.7/ 2.5/ -1.7/ 0<br />
RB11969 ORF1 x<br />
85
Results and Discussion<br />
TABLE 5 Overview of the k-means clustering of the different growth stages of R. baltica. The expression<br />
average of the relevant clusters (row) is shown in colour over the compared growth stages (columns). Red =<br />
induced genes and green = repressed genes.<br />
exponential phase transition phase stationary phase<br />
Cluster-nr. 62 h vs. 44 h 82h vs. 62 h 96 h vs. 82 h 240 h vs. 82 h<br />
4 some<br />
12<br />
17<br />
19<br />
22<br />
13<br />
24<br />
25<br />
18<br />
11 some<br />
23<br />
20 some<br />
16 some<br />
3<br />
21<br />
14 some<br />
Additional files<br />
ADDITIONAL FILE 1 List of differentially expressed genes (ANNEX)<br />
ADDITIONAL FILE 2 List of genes that are involved in the cell division and chromosome<br />
partioning but not regulated in this study<br />
ADDITIONAL FILE 3 List of hypothetical proteins modified after [10] plus additional<br />
expression data<br />
ADDITIONAL FILE 4 List of selected clusters out of the k-means clustering (ANNEX)<br />
86
Results and Discussion<br />
Additional file 2<br />
ID Locus AA Product Gene EC COG<br />
526 RB965 156 membrane protein D<br />
882 RB1641 339 sodium/calcium exchanger protein, D<br />
1145 RB2176 125 hypothetical protein D<br />
1195 RB2264 168 Large-conductance mechanosensitive channel mscL D<br />
1303 RB2434 81 protein containing RNA-binding S4 domain S4 D<br />
1531 RB2833 462 dehydrogenase D<br />
2077 RB3771 222 Putative cyclase D<br />
2241 RB4054 87 hypothetical protein D<br />
2903 RB5151 415 threonine dehydratase, biosynthetic, ilvA 4.3.1.19 D<br />
3162 RB5613 360 protein containing DUF1006 D<br />
3408 RB6045 627 membrane protein D<br />
3628 RB6438 485 secreted protein D<br />
3856 RB6827 966 serine/threonine-protein kinase 2.7.1.37 D<br />
4297 RB7620 49 hypothetical protein D<br />
4966 RB8859 332 D-lactate dehydrogenase ldhA 1.1.1.28 D<br />
5165 RB9239 231 conserved hypothetical protein D<br />
5329 RB9546 276 conserved hypothetical protein, D<br />
5793 RB10397 314 hypothetical protein D<br />
6585 RB11813 80 conserved hypothetical protein D<br />
7263 RB13068 499 conserved hypothetical protein D<br />
745 RB1391 538 cell division inhibitor-<br />
2302 RB4173 337 signal recognition particle-docking protein FtsY<br />
3138 RB5566 728 cell division protein FtsH 3.4.24.-<br />
1607 RB2966 672 cell division protein FtsH 3.4.24.-<br />
3306 RB5871 485 Cell division protein<br />
4490 RB7945 231 glucose-inhibited division protein<br />
5170 RB9255 667 glucose-inhibited division protein gidA<br />
6587 RB11817 937 DNA translocase FtsK<br />
2091 RB3794 1412 FtsK/SpoIIIE family protein<br />
5504 RB9874 267 segregation and condensation gene scpA<br />
3926 RB6962 297 segregation and condensation gene scpB<br />
3418 RB6065 1234 chromosome segregation protein<br />
6463 RB11587 540 segregation and condensation gene scpB<br />
87
Results and Discussion<br />
ADDITIONAL FILE 3<br />
UID Putative function Transcriptome data Proteome data<br />
[10]<br />
Observation,<br />
Dec 2008<br />
Heat<br />
shock<br />
Cold<br />
shock<br />
Salt<br />
shift<br />
Life<br />
cycle<br />
Life<br />
cycle<br />
[10]<br />
Master<br />
gel<br />
[47]<br />
Carbohydrates<br />
[33]<br />
RB1838 Unknown +<br />
RB3342 Unknown LmbE-like<br />
+ + +<br />
protein<br />
Mastergel<br />
[48]<br />
RB4347 Unknown + + + + + + cell wall<br />
RB6328-1 Unknown Adenylate + + + + +<br />
cyclase<br />
RB6328-2 Unknown Adenylate<br />
+<br />
cyclase<br />
RB8246 Unknown + + + cell wall<br />
RB11010 Unknown secreted + + + + most<br />
abundant<br />
RB11107-<br />
1<br />
Unknown + + + most<br />
abundant<br />
RB11107- Unknown +<br />
2<br />
RB11107- Unknown +<br />
3<br />
RB12073 Unknown + +<br />
RB12301 Unknown conserved + most<br />
abundant<br />
RB12323 Unknown conserved + + +<br />
Unique<br />
RB68 Unknown + +<br />
RB512 Unknown + + most<br />
abundant<br />
RB3577 Unknown secreted + +<br />
RB5240-1 Unknown secreted + +<br />
RB5240-2 Unknown +<br />
RB5268 Unknown + +<br />
RB6221 Unknown secreted + + +<br />
RB6941 Unknown secreted + + +<br />
RB7235 Unknown secreted + + + most<br />
abundant<br />
RB8559 Unknown secreted +<br />
RB9512 Unknown secreted +<br />
RB12461 Unknown secreted + + + extracellular<br />
RB12645 Unknown + + extracellular<br />
88
Results and Discussion<br />
3.3 Highly enantioselective sec-alkyl sulfatase activity of the<br />
marine planctomycete Rhodopirellula baltica shows retention of<br />
configuration<br />
Sabine R. Wallner, Marga Bauer, Chris Würdemann, Patricia Wecker, Frank O. Glöckner,<br />
and Kurt Faber<br />
Published in Angewandte Chemie International Edition 44: 2 –4 (2005)<br />
89
Results and Discussion<br />
3.4 Fosmids of novel marine Planctomycetes from the Namibian<br />
and Oregon coast upwelling systems and their cross-comparison<br />
with planctomycete genomes<br />
Authors: Dagmar Woebken, Hanno Teeling, Patricia Wecker, Alexandra Dumitriu, Ivaylo<br />
Kostadinov, Rudolf Amann, Edward F Delong, and Frank Oliver Glöckner 2007<br />
Published in The ISME Journal 1, 419-435 (2007)<br />
90
Summary<br />
4 SUMMARY<br />
In 2003, the completely sequenced and annotated genome of the first planctomycete<br />
Rhodopirellula baltica was published (Glöckner et al. 2003). Besides common genomic<br />
features including Glycolysis, Citrate–cycle or ribosomal RNAs the annotation also revealed<br />
unexpected genetic potential such as a high number of sulphatase genes or genes encoding<br />
enzymes of the C1-metabolism pathway. In conclusion, bioinformatic analysis allowed new<br />
hypotheses to be built about the capabilities and the ecological niche of R. baltica. However,<br />
bioinformatics could not resolve open questions about many features, for example, the<br />
interesting cell-cycle or character and the formation of the holdfast substance. In addition,<br />
bioinformatics could not assign a function to 55% of the predicted ORFs. The annotation<br />
contributed to the understanding of the phylum Planctomycetes but also made clear how poor<br />
our knowledge remains concerning the genetic potential of many environmental organisms.<br />
To turn the information about the genetic potential of an organism into sound biological<br />
knowledge, functional post-genomics using transcriptomics and proteomics are the next<br />
logical step. To further substantiate the predicted functions and get hints for some of the<br />
unknown gene functions, microarray technology was selected. <strong>Whole</strong> genome expression<br />
profiling allows the investigation of thousands of genes in parallel at the mRNA level in a<br />
single experiment. This is a clear advantage compared to other techniques like Real-Time<br />
PCR, which allows investigation of only a rather small set of genes.<br />
During my thesis I set up a pipeline (2.3) for the first planctomycete-specific whole genome<br />
array. This included testing and selection of the slide format and its chemistry. Protocols for<br />
RNA isolation, cDNA synthesis and labelling, required adaptation due to the specific<br />
proteinaceous cell wall of R. baltica. Hybridisation and washing buffer composition as well as<br />
hybridisation time and conditions were adjusted for an optimal signal to background ratio<br />
based on an in-house automatic hybridisation machine (Tecan HS400). The quality of the<br />
array hybridisation was validated by checking for the negative, positive and stringency<br />
controls printed on the array. The pipeline also included the set up of three chemostats for<br />
improving the R. baltica culturing compared to the initially used batch culture systems.<br />
The processing of the resulting expression data included reviewing different data<br />
normalisation approaches and selection of the one which fits the experimental design. For the<br />
analysis of the normalised and filtered expression data, various microarray data analysis tools<br />
were screened. Finally, the open source software program TIGR Multiexperiment Viewer<br />
91
Summary<br />
(MeV) was chosen. This incorporates sophisticated algorithms for clustering, visualisation,<br />
classification, statistical analysis and biological interpretation.<br />
The successful establishment of microarray technology led to the second part of the thesis<br />
dealing with whole genome array expression profiling to address specific questions. The<br />
whole genome array was applied to R. baltica cells exposed to different temperatures and high<br />
salinity in order to learn more about the common and specific stress responses of this<br />
organism (Wecker et al. 2009in prep). The whole genome array was also applied to R. baltica<br />
cells in different growth stages with the aim to investigate and analyse unknown mechanisms<br />
during the cell-cycle (Wecker et al. 2009 in prep).<br />
The following section provides a brief summary of the (main) results obtained in this thesis.<br />
For details, please refer to the original publications which can be found in chapter 3.<br />
4.1 Transcriptional response of the model planctomycete<br />
Rhodopirellula baltica SH1 T to changing environmental conditions<br />
The first manuscript introduces the first whole genome array for any member of the phylum<br />
Planctomycetes, specifically Rhodopirellula baltica. The successful establishment of a<br />
complete microarray pipeline is demonstrated by a model system based on comparisons of the<br />
behaviour of R. baltica cells at different environmental stress factors at the transcriptome<br />
level.<br />
The first experiments focused on the better understanding of the adaptations of R. baltica<br />
grown under changing environmental conditions, e.g. variations in temperature and high<br />
salinity. The rationale behind these experiments is the observation that R. baltica can be<br />
isolated from various marine and brackish habitats while bioinformatic analyses of the<br />
genome support the hypothesis that R. baltica passes through a halocline while passing from<br />
the surface to the sediment. Stress responses to salinity and temperature shifts were monitored<br />
in time series experiments. Cultures grown on mineral media at 28°C were compared to<br />
cultures that were shifted to either elevated (37°C) or reduced (6°C) temperatures or high<br />
salinity (59.5‰) over 300 min.<br />
Transcriptional profiling confirmed that R. baltica is highly responsive to its environment.<br />
Heat shock showed the induction of several known chaperone genes while cold shock altered<br />
the expression of genes involved in lipid metabolism and stress protein production. High<br />
salinity resulted in the modulation of genes coding for compatible solutes, ion transporters<br />
and morphology. We were also able to detect a shared environmental stress response of<br />
92
Summary<br />
R. baltica. Results show the induction of known general stress response genes. The ribosomal<br />
machinery is repressed and is probably functioning as a general stress sensor. Transposase<br />
genes are induced to enhance this genetic activity through rearrangement of the genome.<br />
Noteworthy is the shared induction of three ECF- sigma factors over all three experiments,<br />
which led to the assumption that they are part of the environmental stress signalling pathway.<br />
Another highlight is the regulation of several sulphatase genes in all experiments. We propose<br />
a role for these in the remodelling of the cell wall of R. baltica and point out the diverse role<br />
of the sulphatases. Nearly 50% of all regulated genes have no known function. The<br />
combination of transcriptomics and proteomics points to a set of genes of unknown functions<br />
most probably involved in the global stress response. In summary this work gave new insights<br />
into the complex adaptation machinery of an environmentally relevant marine bacterium. This<br />
work lays the foundation for further bioinformatic and genetic studies, which leads to a<br />
comprehensive understanding of the biology of a model marine Planctomycete.<br />
The corresponding paper is submitted to BMC Genomics.<br />
4.2 The Rhodopirellula baltica life-cycle: Growth-dependent<br />
expression profiling of the complete genome with microarrays<br />
The focus of the second manuscript is on the expression profiling of different growth stages of<br />
the marine planctomycete Rhodopirellula baltica. Although synchronised cell cultures of<br />
R. baltica were not available, it was possible to get first idea about the genes involved in the<br />
characteristic morphological stage of Planctomycetes.<br />
The marine planctomycete R. baltica has a life-cycle consisting of motile swarmer cells and<br />
sessile adult cells. During the genome annotation none of the known genes involved in the<br />
cell-cycle or cell division were found. The life-cycle must therefore have a so far unknown<br />
control mechanism. To find out more about the genes involved in the life-cycle, a whole<br />
genome array for R. baltica was used for the first time. R. baltica cell samples were taken at<br />
different growth stages from a R. baltica culture and growth dependent expression profiling<br />
was carried out. The number of regulated genes increased from 90 exponentially to 863 in the<br />
late stationary phase when compared to the transition phase. Results indicate a remodelling of<br />
the cell wall and membrane in the exponential phase, sensing of environmental changes in the<br />
transition phase, followed by a stationary-phase specific response. 12 out of 110 sulphatases<br />
genes showed a growth-dependent expression pattern. This indicates that some of them might<br />
also be involved in basic cell functionalities such as the remodelling of cell wall components<br />
during the life-cycle. It is also noteworthy that genes associated with the C1-metabolism<br />
93
Summary<br />
appear to be regulated. More than half of the regulated genes per growth stage were annotated<br />
as hypothetical proteins. These genes are likely candidates for functions associated with<br />
planctomycete-specific morphology and/or morphotype-differentiation during the life-cycle.<br />
In summary, the whole genome array of R. baltica gave initial insights into the regulation of<br />
genes during the life-cycle. The transcriptomic results are contributing to a better<br />
understanding of the so far unknown molecular cell biology underlying the cell<br />
compartmentalisation of the phylum Planctomycetales and maybe even of the PVC<br />
superphylum.<br />
The corresponding paper is in preparation.<br />
4.3 Highyl enantioselective sec-alkyl sulfatase activity of the<br />
marine planctomycete Rhodopirellula baltica shows retention of<br />
configuration<br />
The preliminary results of the partial genome array targeting sulphatase genes (C.<br />
Würdemann unpublished results) and the parallel investigations concerning the sulphatase<br />
activity in R. baltica were the fundamental basis for research aim 2.2.<br />
The annotation process of the complete genome sequence of the marine planctomycete<br />
Rhodopirellula baltica revealed that the genome includes 109 genes assigned as sulphatases.<br />
A subset of 85 sulphatases showed the conserved “core” of the sulphatase consensus motif,<br />
which is required to render activated sulphatase proteins. Each of these 85 sequences<br />
contained the adjacent “auxiliary” part of the consensus motif, either fully conserved or only<br />
slightly modified. The sulphatases are active in resting cells of R. baltica and act through<br />
retention of configuration (shown in collaboration with Prof. Dr. Faber <strong>University</strong> of Graz,<br />
Austria). In general, the enantio preference was shown to be “R”, while “S” enantiomers<br />
remained untouched. R. baltica displayed high activities on linear (w-1)-sulphate esters,<br />
which were readily accepted as substrates. Even sterically demanding sulcatyl sulphate was<br />
converted with good rates, which was neither accepted by an inverting alkyl sulphatase<br />
(termed “RS2”) nor by Sulfolobus spp.. Not surprisingly was the quick conversion of the<br />
primary sulphate ester by one of the large set of sulphatases genes. However, no loss of<br />
sulphatase activity in the protein extract of R. baltica cells grown on various C-sources:<br />
glucose, chondroitin sulphate A and condroitin sulphate C could be seen.<br />
The corresponding paper has been published in Angewandte Chemie International Edition 44:<br />
2 –4 (2005).<br />
94
Summary<br />
4.4 Fosmids of novel marine Planctomycetes from the Namibian<br />
and Oregon coast upwelling systems and their cross-comparison<br />
and their cross-comparison with planctomycete genomes<br />
This metagenomic study of fosmids of novel marine Planctomycetes is a good example of the<br />
knowledge transfer of transcriptome data from model organisms to the ecosystem with the<br />
purpose to understand the role of Planctomycetes in the environment.<br />
The objective of the study was to broaden our knowledge about the genetic potential of<br />
marine Planctomycetes using a cultivation independent approach. Therefore, six fosmids from<br />
two different marine upwelling systems were investigated from metagenome libraries. The<br />
fosmids were compared with all available planctomycete genomic sequences (Rhodopirellula<br />
baltica SH 1 T , Candidatus Kuenenia stuttgartiensis and Gemmata obscuriglobus DSM 2246 T )<br />
including the as yet unpublished near-complete genomes of Blastopirellula marina DSM<br />
3645(T) and Planctomyces maris DSM 8797(T). Comparative sequence analysis revealed the<br />
following: A high number of genes coding for sulphatases in all genomes from marine<br />
Planctomycetes. R. baltica harbours the highest number (109), while the freshwater<br />
planctomycete Gemmata obscuriglobus and Candidatus Kueninia stuttgartiensis included<br />
only 12 and 3 sulphatases, respectively. Additionally two fosmids (6FN and 5H12) contained<br />
each one sulphatase. The high number of sulfatase genes in all genomes and their presence on<br />
the fosmids underlined again the specialication of Planctomycetes in carbohydrate<br />
degradation.<br />
Genes involved in the C1-metabolism were found on all Planctomycete genomes, except of<br />
Candidatus Kuenenia stuttgartiensis, and on two fosmids. Their role in the life-style of the<br />
Planctomycetes is so far unknown. However expression profiling revealed that even in the<br />
absence of C1 compounds seven of the respective genes are expressed, which indicates an<br />
active role in the central metabolism of R. baltica.<br />
Genes that are only present in planctomycete genomes and lack similarities with other<br />
bacterial or archaeal genes were present in all five sequenced genomes and on all six fosmids.<br />
It can be speculated that they might be involved in the unique cellular features or adaptations<br />
of Planctomycetes.<br />
In summary, the comparison of genomic information from cultivated and uncultivated<br />
Planctomycetes offered new insights in their physiological capabilities with a focus on<br />
planctomycete-specific features and striking metabolic pathways.<br />
The corresponding paper has been published in the ISME Journal 1, 419-435 (2007).<br />
95
Establishing of the Microarray Pipeline<br />
5 ESTABLISHING THE MICROARRAY PIPELINE<br />
Comparing the results obtained and the initial outline of in my proposal, some changes<br />
become obvious. During the initiation of this PhD thesis it was assumed that only small<br />
methodological adaptations were needed to use whole genome arrays for expression profiling<br />
in Rhodopirellula baltica. However, first tests showed unexpected challenges, like sample<br />
processing or increased background. Optimisations also included the use of chemostat<br />
reactors for the cultivation of R. baltica. This was triggered by two main requirements.<br />
Firstly, the amount of cells needed for microarray experiments could not be obtained by<br />
standard (batch) cultivation approaches. At least three biological independent replicates are<br />
needed to obtain consistent regulation data. Second, synchronised cells are required to assure<br />
reproducibility of the experiments.<br />
After the microarray pipeline was finally running in-house, the reliability needed to be<br />
optimised. Due to the unique character of the Planctomycetes there were no existing<br />
transcriptome studies of other Planctomycetes available for validating the results. Therefore, I<br />
performed expression profiling on the response of R. baltica to different stressors<br />
(temperature and high salinity) to check if the results matched the expectations and to provide<br />
new information on the environmental adaptation of R. baltica. Consequently research aim<br />
2.2 was postponed. In addition, sharing cultures to conduct complementary proteome studies<br />
with our collaboration partner at the Institute of Marine Biotechnology in Greifswald was<br />
initiated. The integration of transcriptome and proteome data is expected to deliver a better<br />
picture about the existence, annotation, regulation and expression of the genes in the<br />
R. baltica.<br />
Our aim was and is to combine our transcriptome results with all publicly available data of<br />
regulated genes in R. baltica. Unfortunately, all results so far were not consistently stored in a<br />
computer readable electronic format. Therefore a beta-version of an R. baltica database was<br />
set up where information on the organism and genes can be stored. The software tool<br />
JCOAST (Richter et al. 2008), which was already used in-house for comparative genomics on<br />
the four planctomycetes genomes and their statistical analysis, was extended to host additional<br />
information about each gene and soon it will be able to show expression data of all<br />
transcriptome studies. All published results on each gene have been integrated into this<br />
internal database. This also includes the latest results of Jerome Dabin from the marine station<br />
in Roscoff (France). They are currently evaluating the catalytic potential of R. baltica with<br />
respect to degradation of carbohydrates. This also includes the re-annotation of 128<br />
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Establishing of the Microarray Pipeline<br />
polysaccharidases in the genome of R. baltica done by experts. Four out of these 128 proteins<br />
were chosen for further functional and structural studies, identified by potential biotechnical<br />
applications and originality. Recently, Dabin et al. (Dabin et al. 2008) published the results of<br />
the crystallisation and preliminary results off x-ray analysis of a pectin lyase RB5312. The<br />
expert re-annotation demonstrated that any genome annotation is only as good as the<br />
knowledge of the annotator. The annotation sets the basis for all further knowledge<br />
extrapolation. Therefore, part of the whole genome pipeline should include curation of the<br />
annotation and a regular automatic update of the annotation against the current databases. At<br />
least the awareness of partially incorrect annotations should be present while generating<br />
hypothesis out of the significantly expressed genes.<br />
In summary, more time than expected had to be invested in method optimisation, resulting in<br />
a reduction of the time available to generate results on the ecology and biology of R. baltica.<br />
97
Contribution to the Research on Planctomycetes<br />
6 CONTRIBUTION TO THE RESEARCH ON<br />
PLANCTOMYCETES<br />
Planctomycetes are a group of budding, peptidoglycan-free bacteria, which attract more and<br />
more attention within the fields of microbial ecology, cell biology and genomics. Studies of<br />
cultured isolates and clone library sequences from natural communities contribute to this<br />
development. The phylogeny of Planctomycetes has been under debate for several years now<br />
(Stackebrandt et al. 1984; Woese 1987; Fuerst 1995; Brochier and Phillippe 2002; Teeling et<br />
al. 2004). Based on genomic information they have been successfully allocated to the PVC<br />
superphylum (Teeling et al. 2004; Fieseler et al. 2006; Wagner and Horn 2006). Their rather<br />
uncommon features such as compartmentalised cell organisation (Fuerst 2005), the ability of<br />
some species to grow chemolithoheterotroph via oxidation of ammonium (Jetten et al. 1997),<br />
their possession of large genomes with partly unexpected genetic potential (Glöckner et al.<br />
2003; Woebken et al. 2007), combined with their wide distribution in a variety of habitats,<br />
has further reinforced a steadily increasing scientific and industrial interest.<br />
Since the start of my thesis many more scientific studies on different Planctomycetes were<br />
carried out highlighting even more how fascinating this phylum is. However none of these<br />
have revealed details of underlying genetic mechanisms:<br />
First of all more planctomycete isolates were published (Kulichevskaya et al. 2007;<br />
Kulichevskaya et al. 2008). The latest diversity studies show that Planctomycetes can be<br />
found in the intertidal sediments of the North Sea (Musat et al. 2006), in the north American<br />
coast diatom bloom (Morris et al. 2006), in the anoxic laxer of Sphagnum peat bog (Ivanova<br />
and Dedysh 2006) and in the alkaline gut of soil-feeding termites (Köhler et al. 2008). Shu et<br />
al. (Shu and Jiao 2008) showed for the first time the systematic assessment of Planctomycetes<br />
in latitudinal surface seawater of the open sea and comparative diversity patterns between<br />
surface seawater and sediments. Latter Planctomycetes show a higher diversity. Looking at<br />
the environmental factors, Shu proposes an influence of high organic carbon and oxygen on<br />
the planctomycete diversity. Even a new metabolism for Planctomycete living in anaerobic<br />
habitats has been proposed (Elshahed et al. 2007). Elshahed et al. (Elshahed et al. 2007)<br />
assumes that the organisms either reduce sulphur via the annotated polysulfide reductase,<br />
although the reducing of elementar sulphur has not been reported so far, or through sugar<br />
fermentation. This is supported by the annotated genes for the lactate acid fermentation<br />
pathway.<br />
98
Contribution to the Research on Planctomycetes<br />
Lately, new techniques were applied to get a better understanding of the planctomycete<br />
morphology. Compartment organisation and chromatin structure of Gemmata obscuriglobus<br />
were explored by cyro-electron tomography (Lieber et al. 2008). Lee et al. (Lee et al. 2009)<br />
showed that the compartmentalised cell plan of members of the Planctomycetes can also be<br />
found in the phylum Verrucomicrobia, belonging to the PVC superphylum. Interesting is also<br />
a recent microscopic study where the morphological changes of the planctomycete Gemmata<br />
obscuriglobus during the cell-cycle was investigated (Lee et al. 2009). This study might be a<br />
good basis for further genetic studies to elucidate the responsible genes. The entire mentioned<br />
studied have provided a wealth of new information about Planctomycetes.<br />
However this work is the first to address the distinct features of Planctomycetes at the<br />
genomic level. It is intended to bridge the gap between classical studies on the cell biology<br />
and physiology of Planctomycetes and their active genetic repertoire.<br />
The genomic information of uncultured Planctomycetes was addressed by the metagenomics<br />
approach to get a clue of their environmental role. The fosmid libraries supported the<br />
hypothesis that Planctomyces play an active role as a carbohydrate degrader (Woebken et al.<br />
2007). Highlight of the comparison of the four complete closed planctomycete genomes were<br />
the high amount of shared planctomycete-specific genes without known functions (Woebken<br />
et al. 2007).<br />
Within this work the first whole genome microarray for a cultured Planctomycete has been<br />
established. This pipeline comprises reproducible and controlled cultivation of R. baltica´s<br />
cultures, a protocol for expression profiling on the transcriptome level, data analysis and<br />
evaluation up to data integration and comparison. Taken as a tool it helps to get hints on the<br />
huge amount of genes in R. baltica currently lacking functional assignments, it elucidates the<br />
function of annotated genes and suggests genes of interest for further functional analysis or<br />
biotechnological applications.<br />
In this work we gained results on certain genes with unknown function that probably involved<br />
in the morphological changes or in the regulation of the cell-cycle and stress response of<br />
R. baltica. For the first time we obtained information about the function of diverse<br />
planctomycete-specific genes that are active under stress. We were able to highlight the<br />
diverse functions of sulphatase genes in the genome of R. baltica. Results showed activity of<br />
genes associated with the C1-metabolism. Finally, we gained knowledge about the behaviour<br />
of R. baltica in its natural habitat. The possibility of transferring this knowledge to the<br />
phylum Planctomycetes has a major impact for our understanding of the potential ecological<br />
roles and adaptations of Planctomycetes in marine environments.<br />
99
Outlook<br />
7 OUTLOOK<br />
Expression profiling: microarray vs. sequencing technology<br />
Recently, a manuscript by Shendure (Shendure 2008) with the controversial title “The<br />
beginning of the end for microarrays” was published in Nature. The authors claimed that<br />
microarrays will be replaced by the faster and cheaper sequencing technologies. The era of<br />
“next generation sequencing technologies” has been heralded with the introduction of the 454<br />
pyrosequencing system in 2005 (Margulies et al. 2005). Since then, four technology platforms<br />
have entered the market (Roche 454, Illumina <strong>Genome</strong> Analyzer, ABI SOLiD and Helicos<br />
HeliScope) and especially the Roche 454 GS FLX sequencing platform is now heavily used<br />
for bacterial genome and small RNA sequencing, metagenomics, genomic structure variation<br />
and human whole genome sequencing (Rothberg and Leamon 2008). Massively parallel 454<br />
pyrosequencing has become a feasible method for increasing sequencing depth and coverage<br />
while reducing time, labour, and cost (Margulies et al. 2005; Moore et al. 2006; Wicker et al.<br />
2006; Huse et al. 2007). Typical output from a 4.5 h run of the original Roche 454 GS 20<br />
sequencer are 20–30 Mb, comprising roughly 300,000 sequence reads averaging 100 bp. With<br />
state of the art machines read length of 250 bp (FLX) and 400 bp (FLX Titanium) can be<br />
routinely reached, increasing the amount of basepairs per run drastically.<br />
Lately, also transcriptomes were analysed by tag sequencing approaches (Cristobal-Vera et al.<br />
2008; Marioni et al. 2008) the former exclusive field of DNA microarrays. In contrast to<br />
microarrays, new sequencing technologies do not rely on the availability of a sequenced<br />
genome and also do not suffer from severe methodological limitations as mentioned already<br />
in this thesis. In general, for tag sequencing a population of RNA (total or only mRNA) is<br />
converted to a library of cDNA fragments with adaptors attached to one or both ends. Each<br />
molecule, with or without amplification, is then sequenced in a high-throughput manner to<br />
obtain short sequences from one end (single-end sequencing) or both ends (pair-end<br />
sequencing). The reads are typically 30–400 bp, depending on the sequencing technology<br />
used (Wang et al. 2009). A major advantage is that this approach is quantitative, resolving<br />
one of the major limitations of the current microarray based technologies.<br />
Sequencing costs are continuously decreasing and the technique has already become a fast<br />
and cost-effective option for functional genomics, especially in case of mixed microbial<br />
populations (meta-transcriptomics). This development narrows the gap between approaches<br />
based on model organisms offering a genetic model and so far barely investigated organisms,<br />
which are most tractable for ecological and evolutionary studies (Cristobal-Vera et al. 2008).<br />
100
Outlook<br />
However, there will still be important applications for microarrays in the future as pointed out<br />
by Shendure et al. (Shendure 2008). Most likely not for large-scale expression profiling due<br />
to the effectiveness of the new sequencing technologies but for other applications such as<br />
custom genotyping and detection of copy number variation. This is because microarrays allow<br />
us to address such questions in a more targeted way. Also in case of expression profiling there<br />
will be niches for microarrays in the future. In particular to answer specific questions about<br />
specific groups of genes of interest (“partial” genome microarrays), or as a screening tool to<br />
get first information on the gene regulation in model organisms (whole genome array).<br />
Another unique feature might be RNA-targeted microarrays. No cDNA synthesis is required<br />
like for sequencing technology and a huge bias can be avoided (Würdemann et al. 2006).<br />
Interesting is also the comparison of the expanses between one Solexa and one established<br />
R. baltica cDNA-microarray run. A Solexa run with a sequence yield of 30-70 bp costs about<br />
3000 € with up to six different experiments per run (500 € per experiment) plus costs for the<br />
instrument and RNA-isolation. An established R. baltica cDNA microarray run costs around<br />
125 Euro per experiment (per slide) plus 160 Euros per slide for the instruments. Calculations<br />
do not include cost of labour and of computer hardware for data analysis and storage. An<br />
established cDNA microarray is still cheaper than a Solexa run at the moment. However,<br />
sequencing technology is already a serious competitor, concerning the cost, for new<br />
expression profiling projects, meaning which are not established yet.<br />
Finally, there is one major limitation correlated with the new sequencing technologies, which<br />
now produce a mass of raw sequencing data in shortest time. Appropriate bioinformatic<br />
infrastructure is required to handle this flood of data. At the moment, only very few working<br />
groups in the world are really prepared for this task. A complete rethinking is required in this<br />
regard. Despite the complexity of establishing a platform for microarray experiments, this<br />
might be a reason for researchers to stick to microarray technology within the next years.<br />
101
Reference<br />
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Amann and F. O. Glöckner (2007). "Fosmids of novel marine Planctomycetes from<br />
the Namibian and Oregon coast upwelling systems and their cross-comparison with<br />
planctomycete genomes." ISME Journal 1(5): 419-435.<br />
Woese, C. R. (1987). "Bacterial evolution." Mircobiol. Rev. 51: 221-271.<br />
Würdemann, C. (2006). Method development for expression profiling in prokaryotes and the<br />
application in marine environments. School of Engineering and Science. Bremen,<br />
International <strong>University</strong> Bremen: 159.<br />
Würdemann, C., J. Peplies, S. Schübbe, A. Ellrott, D. Schüler and F. O. Glöckner<br />
(2006). "Evaluation of gene expression analysis using RNA-targeted partial genome<br />
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108
Acknowledgement<br />
9 ACKNOWLEDGEMENT<br />
……………………….because you never make it on your own……………………….<br />
I wish to express my sincere gratitude to my supervisor Prof. Dr. Frank Oliver Glöckner, who<br />
guided this work and gave me support and scientific freedom. He was always there for me<br />
when I needed advice.<br />
I wish to thank Prof. Dr. Matthias Ullrich and Prof. Dr. Georgi Muskhelishvili for being<br />
members of my thesis and dissertation committee. I would also like to thank Prof. Dr. Rudolf<br />
Amann for taking part in the evaluation of my work and for his constant support in the<br />
background.<br />
I am grateful to Dr. Christine Klockow and Dr. Marga Bauer for their help, advice and<br />
guidance during my PhD.<br />
I am indebted to Andreas Ellrott, Chris Würdemann and Jörg Peplies for providing<br />
inspiration, friendship and wise words.<br />
I would like to thank all members of the Microbial Genomics Group who helped me during<br />
my PhD work and made science enjoyable. Special thanks to the Michael Richter, Christian<br />
Quast, Jost Waldmann and Elmar Prüsse.<br />
I would like to thank all former and present members of the Department of Molecular<br />
Ecology for their support, discussions, experience, their comradeship and the refreshing<br />
evenings outside of the institute, especially Julia Arnds, Katharina Kohls, Anke Meyerdierks,<br />
Jill Peterson, Caroline Rühland, Martha Schattenhofer, Regina Schauer, Silke Wetzel,<br />
Dagmar Wöbken, Jörg Wulf, and my “Findorff-WG-colleagues” Elke Allers and Claudia<br />
Bergin for just being there.<br />
Special thanks to Jörg Wulf, Silke Wetzel and Nicole Rödiger for their excellent support and<br />
patience in the laboratory.<br />
109
Acknowledgement<br />
Pour cette fructueuse collaboration et les bons moments partagés, je voudrai remercier Gurvan<br />
Michel et Tristan Barbeyron de la Station Biologique de Roscoff.<br />
The Network Marine Genomics Europe with all these fascinating members and meetings that<br />
broaden my horizon deserves special mentioning. I would especially like to thank Mike<br />
Thorndyke, Ulrika Hjelm and Michèle Barbier for supporting my skills. Thank you! Tack så<br />
mycket! Merci beaucoup!<br />
This work would not have been possible without the financial support of the Max-Planck-<br />
Society, the Marine Genomics Europe Network and the <strong>Jacobs</strong> <strong>University</strong> Bremen. This work<br />
was done between June 2005 and March 2009 at the Microbial Genomics Group of the Max<br />
Planck Institute for Marine Microbiology in Bremen.<br />
Zum Schluß möchte ich mich bei meinen Brüder Olaf und Axel, meinem Neffen Johannes<br />
und meinen Eltern Elke und Josef für Ihre Unterstützung bedanken. Worte sind Blätter und<br />
Taten sind Früchte.<br />
Ludovic, merci de m’avoir aider à aller de l’avant et de n’avoir jamais arrêter de croire<br />
fermement en moi. Hey Dude, ca fart!<br />
This work is dedicated to all the wonderful, supportive and helpful people I met in the last six<br />
years!<br />
110
Annex<br />
10 ANNEX<br />
111
Transcriptional response of the model planctomycete<br />
Rhodopirellula baltica SH1 T to changing<br />
environmental conditions<br />
ADDITIONAL FILE 1 –<br />
List of differentially expressed genes for all three stress experiments<br />
ADDITIONAL FILE 2 –<br />
List of selected clusters of the k-means clustering
Heat Induced<br />
10min ID Locus AA Nuc Product Gene EC Start Stop IEP MW(kDa) Strand Intensity Ratio Ratio Stdev %<br />
4296 RB7619 278 837 conserved hypothetical protein 4097010 4096174 5.6 30607 - 5.93 1.68 0.11 6.42<br />
6201 RB11150 385 1158 NADH:flavin oxidoreductase/NADH oxidase 6046862 6048019 4.5 42128 + 6.50 1.51 0.13 8.65<br />
6831 RB12276 110 333 conserved hypothetical protein 6605112 6605444 4.7 11911 + 5.89 3.04 0.21 7.07<br />
4149 RB7375 412 1239 Peptidase T pepT 3.4.11.4 3948831 3947593 4.4 44404 - 5.80 1.56 0.21 13.78<br />
6638 RB11919 671 2016 Dihydroxy-acid and 6-phosphogluconate dehydratase 6417713 6415698 5.3 70685 - 5.33 2.10 0.31 14.88<br />
5972 RB10733 389 1170 Glutathione-dependent formaldehyde dehydrogenase fdh 1.2.1.1 5832679 5833848 4.8 42303 + 6.24 2.55 0.26 10.29<br />
2891 RB5134 208 627 Ham1-like protein 2639251 2639877 5.7 22783 + 5.57 1.85 0.09 5.10<br />
5648 RB10127 468 1407 secreted glucose dehydrogenase 5466726 5465320 4.8 51430 - 5.86 2.19 0.06 2.78<br />
6604 RB11853 229 690 GTP cyclohydrolase I folE 3.5.4.16 6382501 6383190 6.2 25392 + 5.99 2.04 0.13 6.39<br />
6263 RB11253 226 681 CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase 6105171 6104491 6.2 24053 - 5.52 1.51 0.05 3.04<br />
5194 RB9305 360 1083 peptide chain release factor 1 prfA 4974620 4975702 4.9 40794 + 5.92 1.82 0.04 2.03<br />
5195 RB9306 296 891 HemK protein hemK 2.1.1.- 4975706 4976596 4.7 32925 + 5.90 1.51 0.14 9.14<br />
6837 RB12285 224 675 phospholipid N-methyltransferase 2.1.1.17 6609391 6610065 6.8 24262 + 6.10 2.07 0.16 7.70<br />
5606 RB10049 217 654 RNA polymerase ECF-type sigma factor 5419507 5420160 10.1 25205 + 5.93 3.14 0.32 10.24<br />
6090 RB10940 183 552 acetyltransferase 5934856 5935407 6.1 20469 + 5.96 2.16 0.24 11.28<br />
98 RB170 96 291 Transposase IS3/IS911 89333 89623 10.1 11124 + 5.44 2.16 0.08 3.51<br />
382 RB704 96 291 Transposase IS3/IS911 378137 378427 10.1 11124 + 5.62 1.75 0.07 4.12<br />
628 RB1189 96 291 Transposase IS3/IS911 606838 607128 10.1 11124 + 5.45 1.78 0.27 15.14<br />
842 RB1571 564 1695 DNA ligase:ATP-dependent DNA ligase lig 6.5.1.1 803159 804853 5.4 63326 + 5.63 1.97 0.13 6.82<br />
2381 RB4299 96 291 Transposase IS3/IS911 2214533 2214823 9.9 11182 + 5.65 2.37 0.32 13.67<br />
2463 RB4433 162 489 Ferritin and Dps 2277208 2277696 4.3 17757 + 6.65 2.04 0.07 3.38<br />
3320 RB5888 96 291 Transposase IS3/IS911 3087636 3087346 10.1 11124 - 5.61 2.09 0.17 8.12<br />
4555 RB8076 173 522 Holliday junction resolvase YqgF 3.1.-.- 4323454 4323975 5.1 18918 + 5.57 1.50 0.24 15.68<br />
4721 RB8397 708 2127 UvrABC system protein B uvrB 4489596 4487470 6.1 80350 - 5.59 1.75 0.32 18.15<br />
6052 RB10876 839 2520 excinuclease ABC subunit A 5903770 5901251 6.4 90046 - 5.80 1.51 0.11 7.01<br />
6558 RB11749 96 291 Transposase IS3/IS911 6328064 6328354 10.1 11124 + 5.42 2.08 0.28 13.36<br />
6559 RB11750 292 879 integrase 6328357 6329235 10 33354 + 5.75 1.85 0.13 6.80<br />
6690 RB12007 526 1581 deoxyribodipyrimidine photo-lyase phrB 4.1.99.3 6478047 6476467 6.2 61173 - 5.52 1.54 0.08 5.05<br />
7098 RB12746 294 885 Formamidopyrimidine-DNA glycolase mutM 3.2.2.23 6869843 6870727 10.3 32447 + 5.59 3.07 0.18 5.80<br />
7203 RB12940 96 291 Transposase IS3/IS911 6966246 6966536 10.1 11124 + 5.46 1.92 0.23 12.14<br />
7353 RB13225 560 1683 ATP-dependent DNA helicase RecQ 7096300 7097982 9.8 61698 + 6.34 1.89 0.09 4.90<br />
3620 RB6428 164 495 conserved hypothetical protein, secreted 3382816 3382322 6.5 17292 - 7.41 2.96 0.13 4.55<br />
6653 RB11941 427 1284 Glycosyl transferase, family 2 6429975 6428692 7.8 47063 - 5.32 1.73 0.34 19.71<br />
6759 RB12148 708 2127 Tail-specific protease precursor (Protease Re) prc 3.4.21.102 6556800 6554674 4.6 79196 - 5.88 2.20 0.01 0.65<br />
7021 RB12606 428 1287 Bacterial sugar transferase 2.7.8.6 6791866 6790580 10.7 48185 - 5.94 1.90 0.10 5.33<br />
7150 RB12831 264 795 Glycosyltransferase 6921326 6920532 4.6 29501 - 6.09 2.57 0.05 1.86<br />
6425 RB11505 199 600 conserved hypothetical protein, secreted 6221829 6221230 7.5 21660 - 6.13 2.37 0.03 1.45<br />
6603 RB11851 346 1041 Bacterial type II secretion system protein 6381415 6382455 9.3 37954 + 5.93 1.54 0.27 17.37<br />
731 RB1359 629 1890 Protease do precursor degP 3.4.21.- 693488 695377 6.1 66729 + 5.96 2.83 0.08 2.98<br />
3812 RB6751 859 2580 chaperone ClpB clpB 3586111 3588690 5.2 95764 + 6.34 1.94 0.04 2.28<br />
5026 RB8966 660 1983 Chaperonin Cpn60/TCP-1 4777493 4779475 5.3 69819 + 6.56 2.86 0.23 7.89<br />
5027 RB8969 101 306 Chaperonin Cpn10 4779554 4779859 4.9 10628 + 6.39 3.13 0.15 4.68<br />
5028 RB8970 540 1623 GroEL 4779905 4781527 4.8 57516 + 6.74 2.95 0.22 7.56<br />
5029 RB8972 391 1176 Chaperone protein dnaJ dnaJ 4781697 4782872 5.7 42391 + 6.25 1.78 0.11 6.26<br />
5030 RB8974 200 603 GrpE protein grpE 4782869 4783471 4.2 21927 + 5.96 3.18 0.22 6.79<br />
5092 RB9103 899 2700 ATP-dependent Clp protease, ATP-binding subunit ClpB 4866549 4863850 4.8 99439 - 6.05 2.99 0.05 1.69<br />
5094 RB9105 704 2115 Heat shock protein Hsp70 dnaK 4868834 4866720 4.6 76038 - 6.67 3.47 0.36 10.35<br />
5713 RB10242 330 993 Curved DNA-binding protein cbpA 5536118 5535126 6.7 35177 - 5.86 2.74 0.17 6.37<br />
5733 RB10279 151 456 heat shock protein, Hsp20 family GrpE 5557118 5557573 4.9 17072 + 6.40 2.84 0.21 7.33<br />
5734 RB10283 107 324 small heat shock protein 5557737 5558060 4.7 12267 + 6.97 4.58 0.38 8.23<br />
5911 RB10627 93 282 Chaperonin Cpn10 5769148 5769429 3.9 9966 + 7.13 2.27 0.07 3.15<br />
5912 RB10629 539 1620 GroEL 5769500 5771119 4.9 57511 + 6.93 2.99 0.15 4.95<br />
6601 RB11847 243 732 Peptide methionine sulfoxide reductase msrA msrA 1.8.4.6 6379337 6380068 6.5 26839 + 5.98 1.91 0.21 11.14<br />
6619 RB11878 168 507 peptide methionine sulfoxide reductase msrA msrA3 1.8.4.6 6396598 6397104 4.8 19100 + 5.75 2.32 0.26 11.03<br />
6727 RB12080 492 1479 Protein-L-isoaspartate(D-aspartate) O-methyltransferase 2.1.1.77 6517231 6515753 8.9 53385 - 5.57 1.79 0.09 4.76<br />
4754 RB8465 184 555 Ferric-uptake regulator fur 4520236 4519682 9.7 21381 - 5.83 1.92 0.12 6.03<br />
5967 RB10727 276 831 manganese-containing catalase 5830775 5831605 5 30055 + 6.08 2.69 0.16 6.05<br />
6102 RB10965 204 615 conserved hypothetical protein, membrane 5942116 5942730 4.9 21483 + 5.61 1.52 0.05 3.51<br />
708 RB1323 429 1290 Protein of unknown function DUF894, DitE 672945 671656 11.8 45426 - 5.96 2.01 0.03 1.52<br />
3648 RB6469 252 759 ABC transporter ATP-binding protein 3419515 3420273 5.6 27684 + 6.01 2.01 0.08 4.19<br />
4868 RB8679 477 1434 oxidoreductase 4620013 4618580 5.1 51903 - 5.56 1.66 0.14 8.67<br />
5260 RB9420 663 1992 Lipid A export ATP-binding/permease protein msbA 5055368 5053377 9.3 73442 - 6.00 2.11 0.30 14.06<br />
5727 RB10270 537 1614 phytoene dehydrogenase carC 1.14.99.- 5551969 5550356 5.5 60117 - 5.43 2.81 0.23 8.18<br />
6655 RB11943 534 1605 phytoene desaturase 6432376 6430772 5.8 57855 - 5.58 1.64 0.33 19.90<br />
6414 RB11488 265 798 oxidoreductase, short-chain dehydrogenase/reductase family 6213893 6214690 5.1 27687 + 5.54 1.92 0.08 4.38<br />
557 RB1044 269 810 TatD-related deoxyribonuclease 543422 544231 5.2 30726 + 5.84 1.55 0.18 11.86<br />
745 RB1391 538 1617 cell division inhibitor- 710780 709164 7.8 59400 - 6.07 1.84 0.15 8.25<br />
846 RB1577 860 2583 ATP dependent DNA helicase 806289 808871 9.9 95417 + 5.78 2.12 0.21 10.05<br />
952 RB1798 364 1095 methanol dehydrogenase regulatory protein 930673 931767 4.6 40611 + 5.60 1.87 0.24 12.86<br />
3602 RB6395 778 2337 conserved hypothetical protein 3364879 3367215 4.6 85714 + 5.80 1.71 0.04 2.31<br />
3936 RB6982 286 861 ABC transporter ATP-binding protein 3715019 3715879 5 31150 + 5.61 1.73 0.14 7.99<br />
4596 RB8157 364 1095 conserved hypothetical protein 4360483 4359389 5.1 40859 - 5.49 1.59 0.21 13.14<br />
4603 RB8173 339 1020 MoxR protein 4366881 4367900 5.1 37556 + 5.64 2.12 0.45 21.29<br />
4622 RB8214 589 1770 membrane protein 4392948 4391179 4.5 63301 - 5.92 2.04 0.21 10.46<br />
6104 RB10967 341 1026 quinone oxidoreductase-like protein 5944040 5943015 4.5 37393 - 6.36 1.71 0.12 6.97<br />
6106 RB10971 303 912 Short-chain dehydrogenase/reductase SDR 5946406 5945495 9.8 32799 - 6.32 2.05 0.08 3.95<br />
6267 RB11262 331 996 diacylglycerol kinase 6108302 6107307 5 35708 - 6.02 2.26 0.09 3.82<br />
6641 RB11923 231 696 Electron transport protein SCO1/SenC 6419736 6419041 4.3 25792 - 5.60 1.60 0.17 10.76<br />
7369 RB13257 286 861 2-hydroxy-6-ketonona-2,4-dienedioic acid hydrolase bphD 3.7.1.- 7105590 7106450 4.6 32540 + 5.50 2.31 0.13 5.50<br />
3490 RB6207 436 1311 conserved hypothetical protein, secreted 3252449 3253759 4.8 48106 + 6.34 1.65 0.33 20.04<br />
4294 RB7616 221 666 protein containing DUF162 4094752 4094087 4.7 23976 - 5.54 2.27 0.11 4.71<br />
4641 RB8246 266 801 conserved hypothetical protein 4409241 4408441 6 29589 - 5.84 2.50 0.06 2.25<br />
4914 RB8767 291 876 conserved hypothetical protein 4660451 4659576 10.1 33956 - 5.59 1.81 0.06 3.37<br />
6217 RB11176 153 462 protein containing DUF442 6065605 6065144 4.8 17161 - 6.25 1.86 0.14 7.53<br />
6265 RB11258 237 714 conserved hypothetical protein, membrane 6106840 6106127 10.3 26175 - 6.11 1.80 0.15 8.46<br />
6348 RB11399 132 399 Putative thiol-disulphide oxidoreductase DCC 6179167 6179565 6.5 15047 + 6.70 3.28 0.09 2.61<br />
6422 RB11498 446 1341 conserved hypothetical protein 6218361 6219701 5.4 50914 + 6.18 1.66 0.08 4.60<br />
3294 RB5848 140 423 dnaK suppressor protein, 3066328 3065906 4.6 15633 - 6.33 1.75 0.16 8.98<br />
6266 RB11260 121 366 dnaK suppressor protein, 6107202 6106837 5.2 13639 - 5.99 2.55 0.08 3.28<br />
79 RB138 214 645 Sigma factor, ECF-like 60872 61516 4.8 24057 + 5.40 2.67 0.23 8.52<br />
300 RB520 375 1128 conserved hypothetical protein 284989 286116 4.8 41300 + 6.06 1.73 0.12 6.85<br />
301 RB521 63 192 hypothetical protein 286399 286208 10.7 7040 - 6.02 2.31 0.49 21.24<br />
316 RB546 485 1458 secreted protein containing DUF1501 302834 304291 7.8 53385 + 6.08 1.86 0.17 9.14<br />
319 RB550 137 414 secreted protein 306709 306296 12.2 15112 - 5.75 1.65 0.20 12.43<br />
358 RB655 346 1041 conserved hypothetical protein, UCP012641 family 354993 353953 5.2 38936 - 6.54 1.74 0.18 10.28<br />
362 RB663 79 240 hypothetical protein 357608 357369 11.4 8792 - 6.39 1.71 0.20 11.59<br />
366 RB671 122 369 hypothetical protein 359218 359586 10.7 13383 + 6.22 1.80 0.31 17.13<br />
580 RB1095 525 1578 hypothetical protein 567469 569046 4.5 57051 + 5.80 2.27 0.11 4.78<br />
662 RB1239 155 468 hypothetical protein 632500 632033 9.7 17106 - 5.80 2.22 0.10 4.34<br />
707 RB1322 35 108 hypothetical protein 671526 671633 12.8 3914 + 5.82 2.45 0.17 6.83<br />
709 RB1324 77 234 hypothetical protein 673169 672936 10.9 8459 - 5.72 1.69 0.08 4.76<br />
747 RB1394 78 237 hypothetical protein 711994 711758 10.1 8765 - 5.64 1.68 0.20 11.85<br />
748 RB1395 319 960 secreted protein 712097 713056 5.5 35212 + 6.35 2.10 0.09 4.31<br />
869 RB1618 97 294 hypothetical protein 833743 834036 5.3 11108 + 5.64 1.87 0.28 14.76<br />
915 RB1728 285 858 membrane protein containing DUF1206 899954 900811 9.9 30055 + 6.10 2.71 0.19 7.20<br />
916 RB1729 120 363 hypothetical protein 901146 900784 7.7 13145 - 5.58 2.76 0.08 2.88<br />
947 RB1789 243 732 conserved hypothetical protein 925861 926592 9.4 27028 + 5.76 1.60 0.18 10.95<br />
1711 RB3159 73 222 protein containing DUF1590 1629337 1629116 12.1 8119 - 6.16 1.62 0.19 11.68<br />
2351 RB4256 358 1077 conserved hypothetical protein, secreted 2180303 2181379 4.5 37091 + 7.60 1.65 0.06 3.59<br />
2388 RB4306 457 1374 transposase and inactivated derivative 2221207 2219834 8.4 52492 - 5.68 1.74 0.15 8.71<br />
2407 RB4347 156 471 conserved hypothetical protein 2235735 2236205 4.7 17459 + 7.56 1.57 0.08 4.78<br />
2440 RB4397 55 168 protein containing DUF1560 2263976 2264143 10.5 5816 + 5.95 2.96 0.11 3.72<br />
2458 RB4426 60 183 hypothetical protein 2275786 2275604 6.3 6516 - 5.18 1.87 0.30 16.15<br />
2461 RB4429 89 270 conserved hypothetical protein 2275996 2276265 4.9 10034 + 7.01 1.62 0.07 4.07<br />
2465 RB4438 160 483 Pyridoxamine 5'-phosphate oxidase- 2278247 2278729 4.4 18043 + 6.37 1.77 0.07 3.88<br />
2609 RB4679 175 528 secreted protein 2391214 2391741 4.2 18596 + 6.73 1.56 0.17 11.03<br />
2951 RB5238 73 222 hypothetical protein 2697775 2697996 10.6 7866 + 5.78 2.22 0.24 10.71<br />
3010 RB5348 457 1374 transposase and inactivated derivative 2765787 2764414 8.4 52492 - 5.86 1.52 0.06 4.26<br />
3022 RB5370 309 930 Rhodopirellula transposase 2774826 2773897 5.9 34530 - 5.74 2.09 0.18 8.77<br />
3023 RB5371 109 330 hypothetical protein 2774829 2775158 7.5 12033 + 5.15 1.52 0.15 10.15<br />
3118 RB5523 91 276 hypothetical protein 2860984 2860709 6.3 9939 - 5.87 1.79 0.14 7.58
3130 RB5551 663 1992 hypothetical protein 2889101 2891092 5.7 70932 + 5.30 1.79 0.20 11.03<br />
3139 RB5567 66 201 hypothetical protein 2897766 2897566 12.1 7106 - 5.86 1.82 0.01 0.71<br />
3303 RB5866 441 1326 ISXo8 transposase 3076242 3077567 9.2 50613 + 5.43 1.52 0.37 24.60<br />
3346 RB5938 370 1113 hypothetical protein 3113484 3112372 5.6 41837 - 6.06 2.78 0.17 5.94<br />
3503 RB6233 629 1890 membrane protein 3271181 3269292 5.4 69401 - 6.67 1.73 0.12 6.95<br />
3588 RB6379 153 462 hypothetical protein 3353122 3352661 5.8 17398 - 6.57 1.99 0.08 3.90<br />
3621 RB6429 61 186 hypothetical protein 3382998 3382813 12.1 7095 - 7.20 2.45 0.08 3.22<br />
3784 RB6706 37 114 hypothetical protein 3560942 3560829 7.3 4375 - 5.37 1.61 0.40 25.15<br />
3888 RB6888 420 1263 secreted protein containing DUF1573 3662449 3661187 4.3 45773 - 5.48 2.43 0.26 10.89<br />
3910 RB6928 160 483 hypothetical protein 3678479 3678961 4.1 16965 + 5.56 1.51 0.34 22.85<br />
4151 RB7381 243 732 membrane protein 3950326 3949595 7.4 26253 - 5.51 2.37 0.21 8.75<br />
4497 RB7960 363 1092 ATP:guanido phosphotransferase 2.7.3.- 4257723 4256632 5.4 41235 - 5.74 1.50 0.09 5.75<br />
4516 RB8008 400 1203 secreted protein containing DUF1559 4279356 4280558 6.3 43113 + 5.81 1.52 0.01 0.45<br />
4583 RB8132 177 534 hypothetical protein 4349972 4350505 10.8 19786 + 6.02 2.19 0.09 4.01<br />
4590 RB8146 151 456 hypothetical protein 4355122 4354667 4.3 17016 - 5.99 2.38 0.10 4.11<br />
4595 RB8153 486 1461 conserved hypothetical protein 4359392 4357932 4.8 54199 - 5.28 1.80 0.13 7.04<br />
4639 RB8243 110 333 protein containing DUF1582 4408184 4407852 11.5 12145 - 5.62 1.78 0.06 3.45<br />
4704 RB8365 90 273 membrane protein 4463903 4463631 11.7 9405 - 6.57 1.68 0.13 7.64<br />
4705 RB8367 50 153 hypothetical protein 4463905 4464057 3.7 5636 + 6.21 2.03 0.08 4.14<br />
4836 RB8601 417 1254 hypothetical protein 4588912 4590165 4.2 45382 + 5.60 1.51 0.04 2.40<br />
4852 RB8641 92 279 hypothetical protein 4605970 4606248 7.5 10045 + 5.78 1.88 0.08 4.50<br />
4880 RB8700 58 177 hypothetical protein 4630224 4630400 4.8 6268 + 6.86 1.82 0.05 2.88<br />
5023 RB8959 662 1989 hypothetical protein 4777121 4775133 5.3 70536 - 5.79 2.45 0.21 8.75<br />
5031 RB8975 110 333 hypothetical protein 4783888 4783556 10.4 12068 - 5.93 2.64 0.15 5.64<br />
5157 RB9230 107 324 hypothetical protein 4936673 4936996 9.9 11900 + 5.81 2.43 0.12 4.83<br />
5161 RB9235 265 798 nodulation protein NoeI 4938435 4939232 9.7 29897 + 5.71 1.90 0.15 8.00<br />
5468 RB9805 1226 3681 membrane protein 5276839 5280519 4.1 129222 + 5.81 1.55 0.01 0.35<br />
5473 RB9811 102 309 secreted protein 5284365 5284673 11.7 11169 + 5.41 1.92 0.28 14.46<br />
5474 RB9813 161 486 hypothetical protein 5285123 5284638 12 17657 - 5.52 1.95 0.39 19.78<br />
5547 RB9950 736 2211 conserved hypothetical protein 5359563 5357353 4.2 77138 - 6.07 1.83 0.09 5.05<br />
5550 RB9955 452 1359 secreted protein containing DUF1552 5363605 5362247 5.6 50315 - 5.66 2.20 0.27 12.29<br />
5552 RB9959 65 198 hypothetical protein 5366417 5366220 11.9 7376 - 5.66 2.23 0.32 14.42<br />
5576 RB10007 313 942 conserved hypothetical protein 5390130 5391071 5 34128 + 6.34 2.46 0.02 0.88<br />
5607 RB10051 1210 3633 conserved hypothetical protein 5420157 5423789 4.6 135400 + 5.93 2.47 0.27 11.14<br />
5647 RB10126 43 132 hypothetical protein 5465210 5465341 12.9 4964 + 6.31 1.67 0.07 4.08<br />
5735 RB10286 48 147 hypothetical protein 5558157 5558011 11.9 5389 - 8.92 1.64 0.29 17.92<br />
5738 RB10292 581 1746 conserved hypothetical protein, secreted 5559646 5561391 8.3 66858 + 5.64 2.36 0.15 6.37<br />
5741 RB10299 455 1368 ATP-dependent carboligase related to biotin carboxylase (ORFY) 5563036 5564403 10 49949 + 6.12 2.41 0.15 6.13<br />
5750 RB10319 65 198 hypothetical protein 5577574 5577771 9.6 7480 + 5.59 1.63 0.05 3.05<br />
5771 RB10350 163 492 Thioredoxin 5596878 5596387 9.7 18371 - 5.79 2.01 0.20 9.74<br />
5785 RB10384 96 291 hypothetical protein 5612174 5611884 4.1 10517 - 6.07 1.53 0.07 4.56<br />
5815 RB10440 49 150 hypothetical protein 5655693 5655842 11.5 5080 + 6.25 1.55 0.07 4.32<br />
5901 RB10607 129 390 hypothetical protein 5759926 5759537 11 14369 - 5.84 1.78 0.24 13.44<br />
5902 RB10608 457 1374 transposase and inactivated derivative 5759903 5761276 8.4 52492 + 5.98 1.85 0.17 9.32<br />
5910 RB10623 60 183 secreted protein 5769140 5768958 3.9 6284 - 7.73 1.78 0.10 5.40<br />
5931 RB10665 200 603 hypothetical protein 5789912 5790514 5.3 21401 + 6.06 1.75 0.24 13.97<br />
5968 RB10728 132 399 secreted protein 5831649 5832047 9.9 13833 + 6.04 2.96 0.23 7.82<br />
5969 RB10729 72 219 hypothetical protein 5832028 5832246 11.2 8203 + 5.82 1.50 0.17 11.14<br />
5970 RB10730 45 138 hypothetical protein 5832453 5832590 10.4 4921 + 5.11 1.62 0.28 17.45<br />
6053 RB10877 453 1362 membrane protein 5905128 5903767 4.3 48882 - 5.42 2.42 0.30 12.49<br />
6067 RB10896 161 486 secreted protein 5917040 5916555 10 17034 - 5.75 2.10 0.16 7.54<br />
6087 RB10936 56 171 hypothetical protein 5934348 5934518 12.5 6620 + 5.58 2.64 0.09 3.49<br />
6088 RB10937 61 186 hypothetical protein 5934561 5934376 9.7 6825 - 5.84 2.34 0.27 11.44<br />
6094 RB10948 153 462 conserved hypothetical protein, secreted 5938575 5939036 4.2 17070 + 8.18 1.94 0.04 1.80<br />
6095 RB10950 233 702 membrane or secreted protein 5939124 5939825 4.9 25130 + 5.98 2.64 0.36 13.48<br />
6097 RB10954 143 432 hypothetical protein 5940749 5940318 10.4 16058 - 6.34 1.78 0.05 2.67<br />
6098 RB10956 117 354 hypothetical protein 5940336 5940689 4.8 13013 + 6.88 2.14 0.14 6.55<br />
6099 RB10957 99 300 conserved hypothetical protein 5940708 5941007 5.6 11604 + 7.41 1.68 0.06 3.66<br />
6100 RB10958 158 477 hypothetical protein 5941015 5941491 5.4 18101 + 7.06 1.60 0.11 6.60<br />
6108 RB10974 234 705 hypothetical protein 5946559 5947263 5.3 25956 + 5.74 2.13 0.17 7.86<br />
6124 RB11000 224 675 conserved hypothetical protein, membrane 5964054 5963380 8.8 24185 - 5.76 1.98 0.07 3.54<br />
6178 RB11110 309 930 Rhodopirellula transposase 6022600 6023529 5.8 34570 + 5.60 1.98 0.22 11.36<br />
6307 RB11323 143 432 conserved hypothetical protein 6142431 6142000 5.4 15695 - 5.64 1.65 0.21 13.05<br />
6346 RB11396 36 111 conserved hypothetical protein 6178445 6178555 11.9 4104 + 7.17 1.51 0.19 12.29<br />
6347 RB11397 207 624 SOUL heme-binding protein 6178540 6179163 8.7 23072 + 6.52 3.24 0.26 8.03<br />
6420 RB11496 67 204 conserved hypothetical protein 6217440 6217643 10.8 7449 + 5.87 2.03 0.04 1.97<br />
6424 RB11504 72 219 conserved hypothetical protein 6221285 6221067 10.7 8212 - 5.78 3.88 0.07 1.92<br />
6455 RB11566 195 588 conserved hypothetical protein 6243111 6243698 10.8 22135 + 5.99 2.45 0.03 1.07<br />
6472 RB11603 37 114 conserved hypothetical protein 6256722 6256835 9 3998 + 6.02 2.03 0.32 15.63<br />
6511 RB11667 174 525 conserved hypothetical protein 6293231 6292707 10 19482 - 5.62 2.15 0.14 6.74<br />
6633 RB11909 168 507 conserved hypothetical protein 6410157 6410663 4.1 18314 + 5.21 1.59 0.33 21.06<br />
6637 RB11918 134 405 protein containing DUF971 6415701 6415297 6.1 14675 - 5.74 1.94 0.20 10.34<br />
6673 RB11977 196 591 conserved hypothetical protein 6455958 6456548 9.5 21922 + 5.26 1.94 0.37 19.05<br />
6718 RB12066 135 408 conserved hypothetical protein 6510147 6510554 10 14924 + 6.80 1.54 0.03 2.11<br />
6719 RB12067 107 324 conserved hypothetical protein 6510236 6510559 11.9 12169 + 5.64 2.71 0.25 9.27<br />
6729 RB12082 244 735 haloacid dehalogenase-type hydrolase 6519608 6518874 4.3 26835 - 5.41 2.18 0.28 12.93<br />
6760 RB12150 53 162 conserved hypothetical protein 6556901 6557062 4.1 5983 + 6.20 2.39 0.18 7.59<br />
6783 RB12196 49 150 conserved hypothetical protein 6577078 6576929 7.4 5280 - 5.40 1.51 0.27 17.94<br />
6810 RB12236 203 612 conserved hypothetical protein, membrane 6590935 6591546 4.8 22076 + 5.78 1.83 0.07 3.83<br />
6817 RB12247 74 225 conserved hypothetical protein 6597048 6596824 6.3 8306 - 6.11 4.03 0.31 7.73<br />
6846 RB12297 169 510 conserved hypothetical protein 6615598 6616107 4.4 17963 + 6.68 1.53 0.09 6.15<br />
6877 RB12347 324 975 hydrolase 6644741 6643767 5.6 35854 - 5.74 1.62 0.33 20.26<br />
6878 RB12348 260 783 FxsA cytoplasmic membrane protein 6645520 6644738 11.6 28277 - 6.05 2.62 0.65 24.76<br />
6889 RB12375 446 1341 conserved hypothetical protein 6659741 6661081 4.9 48379 + 6.19 1.55 0.01 0.94<br />
6947 RB12461 578 1737 conserved hypothetical protein, secreted 6708942 6707206 4.2 61262 - 5.91 1.82 0.22 12.15<br />
6957 RB12484 324 975 conserved hypothetical protein, membrane 6721052 6720078 10.6 34787 - 5.28 1.52 0.11 7.02<br />
7008 RB12580 63 192 conserved hypothetical protein 6780121 6779930 3.8 7136 - 5.63 1.53 0.17 11.24<br />
7148 RB12829 159 480 conserved hypothetical protein 6919813 6920292 9.5 17677 + 5.62 2.43 0.14 5.62<br />
7149 RB12830 102 309 conserved hypothetical protein 6920508 6920200 10.9 11753 - 5.99 2.35 0.08 3.53<br />
7151 RB12833 109 330 conserved hypothetical protein 6921305 6921634 12 12221 + 5.91 2.37 0.05 1.93<br />
7202 RB12936 580 1743 conserved hypothetical protein 6965872 6964130 5.3 66248 - 6.27 2.98 0.17 5.71<br />
7256 RB13053 142 429 secreted protein 7008596 7009024 5.6 14910 + 5.56 1.88 0.14 7.67<br />
7270 RB13082 73 222 conserved hypothetical protein 7020677 7020898 11.7 8266 + 6.12 1.86 0.21 11.16<br />
7271 RB13084 179 540 protein containing DUF820 7020834 7021373 4.5 20200 + 5.25 1.73 0.35 20.12<br />
7293 RB13115 81 246 conserved hypothetical protein 7036614 7036369 9.8 8735 - 6.05 1.63 0.20 12.14<br />
7295 RB13117 156 471 nitrogen regulation protein 7036595 7037065 4.6 17819 + 6.14 1.76 0.03 1.83<br />
7350 RB13221 322 969 secreted protein 7093018 7093986 4.5 35324 + 5.80 2.18 0.12 5.60<br />
7354 RB13228 116 351 conserved hypothetical protein 7097952 7098302 10.4 13570 + 5.92 2.64 0.18 6.72<br />
20min ID Locus AA Nuc Product Gene EC Start Stop IEP MW(kDa) Strand i r stdev %<br />
1961 RB3596 144 435 nitrogen fixation protein (NifU protein) 1868895 1869329 4.3 15958 + 6.04 1.60 0.17 10.80<br />
6831 RB12276 110 333 conserved hypothetical protein 6605112 6605444 4.7 11911 + 6.93 1.60 0.20 12.48<br />
4703 RB8363 401 1206 peptidase pepT 3.4.11.- 4463592 4462387 4.6 42466 - 5.21 1.53 0.20 13.00<br />
6638 RB11919 671 2016 Dihydroxy-acid and 6-phosphogluconate dehydratase 6417713 6415698 5.3 70685 - 6.03 2.40 0.29 12.16<br />
5972 RB10733 389 1170 Glutathione-dependent formaldehyde dehydrogenase fdh 1.2.1.1 5832679 5833848 4.8 42303 + 6.23 1.79 0.18 10.23<br />
1126 RB2144 317 954 g eranylgeranyl pyrophosphate synthetase 1136089 1135136 5 33509 - 6.37 2.02 0.23 11.19<br />
2892 RB5135 137 414 ; PTPS.; Pts-like 6-pyruvoyltetrahydropterin synthase protein 4.2.3.12 2640266 2639853 5.5 15985 - 5.80 1.66 0.41 24.81<br />
3344 RB5934 661 1986 Squalene--hopene cyclase shc 5.4.99.17 3112260 3110275 5.2 72494 - 5.43 1.56 0.26 16.86<br />
5761 RB10335 244 735 Di-trans-poly-cis-decaprenylcistransferase 5590382 5589648 5 28049 - 5.71 1.50 0.19 12.53<br />
1034 RB1977 385 1158 mRNA 3'-end processing factor 1039488 1040645 6 42759 + 5.80 1.85 0.05 2.91<br />
5571 RB9999 281 846 conserved hypothetical protein 5386828 5385983 4.6 29948 - 5.83 1.72 0.42 24.25<br />
5606 RB10049 217 654 RNA polymerase ECF-type sigma factor 5419507 5420160 10.1 25205 + 6.57 2.35 0.03 1.20<br />
98 RB170 96 291 Transposase IS3/IS911 89333 89623 10.1 11124 + 6.05 2.34 0.28 12.02<br />
382 RB704 96 291 Transposase IS3/IS911 378137 378427 10.1 11124 + 6.23 1.52 0.31 20.07<br />
1127 RB2146 139 420 Exonuclease VII, small subunit xseB 3.1.11.6 1136505 1136086 4.2 14778 - 6.27 2.47 0.27 10.80<br />
2135 RB3871 438 1317 transposase of IS5377-like element 2001638 2002954 9.4 48848 + 5.64 2.86 0.30 10.65<br />
2381 RB4299 96 291 Transposase IS3/IS911 2214533 2214823 9.9 11182 + 6.37 1.83 0.38 20.58<br />
2463 RB4433 162 489 Ferritin and Dps 2277208 2277696 4.3 17757 + 6.80 2.09 0.50 23.84<br />
2695 RB4811 438 1317 ISPg4, transposase 2465768 2467084 9.3 48850 + 5.89 3.18 0.39 12.25<br />
3320 RB5888 96 291 Transposase IS3/IS911 3087636 3087346 10.1 11124 - 6.25 1.93 0.26 13.21<br />
5254 RB9411 458 1377 ISPg4, transposase 5048517 5049893 7.8 50772 + 5.80 1.97 0.16 8.31<br />
5472 RB9810 438 1317 transposase of IS5377-like element 5283122 5284438 9.3 48850 + 5.82 2.83 0.32 11.29<br />
6558 RB11749 96 291 Transposase IS3/IS911 6328064 6328354 10.1 11124 + 6.05 2.18 0.33 15.24
7098 RB12746 294 885 Formamidopyrimidine-DNA glycolase mutM 3.2.2.23 6869843 6870727 10.3 32447 + 6.13 2.05 0.16 7.82<br />
7203 RB12940 96 291 Transposase IS3/IS911 6966246 6966536 10.1 11124 + 6.27 1.99 0.27 13.39<br />
3620 RB6428 164 495 conserved hypothetical protein, secreted 3382816 3382322 6.5 17292 - 7.71 2.05 0.33 16.01<br />
7150 RB12831 264 795 Glycosyltransferase 6921326 6920532 4.6 29501 - 6.42 2.11 0.25 12.03<br />
731 RB1359 629 1890 Protease do precursor degP 3.4.21.- 693488 695377 6.1 66729 + 6.51 1.84 0.20 10.71<br />
1197 RB2268 282 849 peptide methionine sulfoxide reductase msrB 1.8.4.6 1191389 1190541 9.9 31115 - 6.26 2.40 0.24 10.17<br />
1607 RB2966 672 2019 cell division protein FtsH ftsH 3.4.24.- 1527063 1529081 5.5 74399 + 6.28 1.86 0.29 15.77<br />
3812 RB6751 859 2580 chaperone ClpB clpB 3586111 3588690 5.2 95764 + 6.95 1.74 0.13 7.34<br />
5026 RB8966 660 1983 Chaperonin Cpn60/TCP-1 4777493 4779475 5.3 69819 + 7.03 2.00 0.18 8.99<br />
5028 RB8970 540 1623 GroEL 4779905 4781527 4.8 57516 + 7.21 2.09 0.08 3.85<br />
5030 RB8974 200 603 GrpE protein grpE 4782869 4783471 4.2 21927 + 6.74 1.76 0.18 9.94<br />
5094 RB9105 704 2115 Heat shock protein Hsp70 dnaK 4868834 4866720 4.6 76038 - 7.36 2.63 0.21 8.05<br />
5734 RB10283 107 324 small heat shock protein 5557737 5558060 4.7 12267 + 7.52 3.65 0.16 4.28<br />
5911 RB10627 93 282 Chaperonin Cpn10 5769148 5769429 3.9 9966 + 7.36 1.73 0.18 10.40<br />
5912 RB10629 539 1620 GroEL 5769500 5771119 4.9 57511 + 7.35 1.95 0.29 15.14<br />
3648 RB6469 252 759 ABC transporter ATP-binding protein 3419515 3420273 5.6 27684 + 6.19 2.43 0.20 8.04<br />
5727 RB10270 537 1614 phytoene dehydrogenase carC 1.14.99.- 5551969 5550356 5.5 60117 - 6.05 2.15 0.10 4.79<br />
3933 RB6973 278 837 tellurite resistance protein 3710584 3711420 5 30569 + 5.76 1.56 0.39 24.75<br />
1497 RB2754 229 690 phosphoglycolate phosphatase gph 3.1.3.18 1436382 1437071 5.3 25345 + 6.77 1.86 0.28 14.81<br />
1608 RB2968 386 1161 GTPase EngC engC 3.6.1.- 1529085 1530245 8.6 41916 + 6.25 1.68 0.14 8.51<br />
2547 RB4579 194 585 Protein of unknown function, UPF0054 2346073 2345489 3.7 20879 - 5.87 1.59 0.22 13.98<br />
3602 RB6395 778 2337 conserved hypothetical protein 3364879 3367215 4.6 85714 + 6.04 2.06 0.23 11.12<br />
3936 RB6982 286 861 ABC transporter ATP-binding protein 3715019 3715879 5 31150 + 5.95 2.07 0.04 2.07<br />
4603 RB8173 339 1020 MoxR protein 4366881 4367900 5.1 37556 + 6.11 1.67 0.29 17.12<br />
6114 RB10985 207 624 HD domain protein 5954633 5954010 5.7 23118 - 6.19 1.93 0.06 3.01<br />
7369 RB13257 286 861 2-hydroxy-6-ketonona-2,4-dienedioic acid hydrolase bphD 3.7.1.- 7105590 7106450 4.6 32540 + 6.04 1.99 0.21 10.30<br />
1502 RB2774 82 249 conserved hypothetical protein 1443786 1444034 4.4 9450 + 6.23 1.88 0.23 12.31<br />
2453 RB4419 178 537 MgtC/SapB transporter 2272912 2273448 6.2 18995 + 6.46 1.77 0.30 16.86<br />
3490 RB6207 436 1311 conserved hypothetical protein, secreted 3252449 3253759 4.8 48106 + 6.60 2.03 0.11 5.51<br />
4294 RB7616 221 666 protein containing DUF162 4094752 4094087 4.7 23976 - 5.92 1.96 0.24 12.27<br />
5032 RB8976 92 279 conserved hypothetical protein 4783568 4783846 9.8 10362 + 5.67 2.49 0.49 19.61<br />
6103 RB10966 60 183 Protein of unknown function, UPF0057 5942934 5942752 10.1 6793 - 6.28 1.54 0.33 21.23<br />
3294 RB5848 140 423 dnaK suppressor protein, 3066328 3065906 4.6 15633 - 6.64 1.67 0.03 1.59<br />
6266 RB11260 121 366 dnaK suppressor protein, 6107202 6106837 5.2 13639 - 6.51 1.66 0.04 2.66<br />
79 RB138 214 645 Sigma factor, ECF-like 60872 61516 4.8 24057 + 6.14 2.15 0.30 13.87<br />
299 RB519 54 165 hypothetical protein 285036 284872 10.6 6171 - 5.88 3.40 0.15 4.49<br />
300 RB520 375 1128 conserved hypothetical protein 284989 286116 4.8 41300 + 6.02 2.32 0.29 12.52<br />
301 RB521 63 192 hypothetical protein 286399 286208 10.7 7040 - 6.24 3.53 0.14 4.01<br />
316 RB546 485 1458 secreted protein containing DUF1501 302834 304291 7.8 53385 + 5.95 2.19 0.25 11.52<br />
319 RB550 137 414 secreted protein 306709 306296 12.2 15112 - 5.98 2.18 0.24 10.96<br />
358 RB655 346 1041 conserved hypothetical protein, UCP012641 family 354993 353953 5.2 38936 - 6.56 1.62 0.25 15.35<br />
362 RB663 79 240 hypothetical protein 357608 357369 11.4 8792 - 6.61 2.53 0.28 11.25<br />
500 RB924 208 627 hypothetical protein 490400 489774 12.2 22548 - 5.58 1.89 0.23 12.19<br />
670 RB1256 51 156 hypothetical protein 641044 640889 12 5840 - 5.44 1.77 0.20 11.07<br />
913 RB1722 651 1956 hypothetical protein 897688 899643 5.4 70377 + 6.20 2.57 0.26 9.96<br />
915 RB1728 285 858 membrane protein containing DUF1206 899954 900811 9.9 30055 + 6.45 1.67 0.31 18.60<br />
916 RB1729 120 363 hypothetical protein 901146 900784 7.7 13145 - 6.12 2.09 0.11 5.29<br />
1028 RB1961 117 354 hypothetical protein 1033791 1034144 11.9 13392 + 6.76 1.63 0.16 10.08<br />
1435 RB2649 53 162 hypothetical protein 1374987 1375148 9.1 5813 + 6.14 1.76 0.19 10.71<br />
1461 RB2697 508 1527 protein containing DUF1501 1402745 1404271 6.9 56235 + 5.99 1.87 0.16 8.34<br />
1477 RB2724 266 801 membrane protein 1419817 1419017 7.4 29523 - 6.25 1.88 0.13 7.11<br />
1506 RB2784 203 612 membrane protein containing DUF107 1448065 1447454 4.5 21076 - 6.04 1.99 0.21 10.47<br />
1515 RB2804 201 606 hypothetical protein 1458645 1458040 5.3 22766 - 6.17 1.63 0.16 9.93<br />
1516 RB2806 41 126 hypothetical protein 1458677 1458802 6.5 4364 + 5.73 1.58 0.29 18.31<br />
1518 RB2809 226 681 hypothetical protein 1459745 1459065 9.1 25318 - 6.02 2.28 0.13 5.53<br />
1606 RB2963 119 360 hypothetical protein 1526707 1527066 12.2 13440 + 6.24 2.31 0.01 0.40<br />
1728 RB3196 109 330 hypothetical protein 1650093 1650422 10.1 12287 + 6.59 2.98 0.37 12.55<br />
2304 RB4176 96 291 hypothetical protein 2146928 2146638 12.4 11402 - 6.30 1.70 0.14 8.38<br />
2440 RB4397 55 168 protein containing DUF1560 2263976 2264143 10.5 5816 + 6.59 1.84 0.17 9.41<br />
2443 RB4400 58 177 hypothetical protein 2265382 2265206 9 6610 - 6.08 1.75 0.03 1.96<br />
2461 RB4429 89 270 conserved hypothetical protein 2275996 2276265 4.9 10034 + 7.40 1.57 0.25 15.68<br />
2465 RB4438 160 483 Pyridoxamine 5'-phosphate oxidase- 2278247 2278729 4.4 18043 + 6.42 2.65 0.20 7.48<br />
2509 RB4510 49 150 hypothetical protein 2307657 2307508 9 5554 - 6.23 1.56 0.28 17.80<br />
2608 RB4678 199 600 secreted protein 2390618 2391217 4.2 20927 + 6.34 1.84 0.28 15.16<br />
2696 RB4812 92 279 secreted protein 2467011 2467289 12.5 10014 + 5.81 2.51 0.29 11.54<br />
2951 RB5238 73 222 hypothetical protein 2697775 2697996 10.6 7866 + 6.01 2.97 0.25 8.31<br />
3022 RB5370 309 930 Rhodopirellula transposase 2774826 2773897 5.9 34530 - 6.24 1.52 0.16 10.75<br />
3118 RB5523 91 276 hypothetical protein 2860984 2860709 6.3 9939 - 6.06 2.07 0.10 4.64<br />
3295 RB5850 63 192 hypothetical protein 3066498 3066307 5.8 7096 - 6.41 1.97 0.23 11.73<br />
3346 RB5938 370 1113 hypothetical protein 3113484 3112372 5.6 41837 - 6.57 3.47 0.33 9.43<br />
3457 RB6144 38 117 hypothetical protein 3228305 3228189 11.1 4314 - 5.90 1.81 0.32 17.50<br />
3588 RB6379 153 462 hypothetical protein 3353122 3352661 5.8 17398 - 6.94 1.61 0.14 8.86<br />
3621 RB6429 61 186 hypothetical protein 3382998 3382813 12.1 7095 - 7.40 2.04 0.40 19.74<br />
3746 RB6637 50 153 hypothetical protein 3528317 3528469 10.1 5841 + 5.78 1.72 0.33 19.16<br />
3771 RB6685 72 219 hypothetical protein 3554265 3554047 12.2 8077 - 5.86 2.00 0.14 7.22<br />
3784 RB6706 37 114 hypothetical protein 3560942 3560829 7.3 4375 - 6.05 1.64 0.14 8.45<br />
3803 RB6735 50 153 hypothetical protein 3577540 3577388 9.9 5867 - 5.59 1.53 0.13 8.64<br />
3888 RB6888 420 1263 secreted protein containing DUF1573 3662449 3661187 4.3 45773 - 5.78 2.14 0.18 8.54<br />
4151 RB7381 243 732 membrane protein 3950326 3949595 7.4 26253 - 6.00 2.34 0.15 6.63<br />
4590 RB8146 151 456 hypothetical protein 4355122 4354667 4.3 17016 - 6.60 1.90 0.32 16.87<br />
4595 RB8153 486 1461 conserved hypothetical protein 4359392 4357932 4.8 54199 - 5.72 1.70 0.37 21.80<br />
4606 RB8177 1318 3957 membrane protein 4371052 4375008 4.3 148031 + 5.80 1.53 0.28 18.15<br />
4704 RB8365 90 273 membrane protein 4463903 4463631 11.7 9405 - 6.59 2.07 0.34 16.46<br />
4727 RB8409 97 294 hypothetical protein 4495547 4495840 8.9 10905 + 5.76 1.62 0.04 2.58<br />
5038 RB8987 48 147 hypothetical protein 4790856 4790710 9.1 5309 - 5.83 2.38 0.14 6.05<br />
5157 RB9230 107 324 hypothetical protein 4936673 4936996 9.9 11900 + 6.39 1.64 0.36 22.20<br />
5456 RB9787 61 186 hypothetical protein 5270904 5270719 6.1 6701 - 5.97 2.32 0.22 9.36<br />
5473 RB9811 102 309 secreted protein 5284365 5284673 11.7 11169 + 6.11 2.37 0.25 10.74<br />
5524 RB9907 433 1302 ISXo8 transposase 5338185 5339486 9.4 49646 + 6.12 1.83 0.11 5.86<br />
5550 RB9955 452 1359 secreted protein containing DUF1552 5363605 5362247 5.6 50315 - 6.34 1.61 0.27 16.64<br />
5654 RB10137 73 222 protein containing DUF1590 5469772 5469551 12.1 8049 - 5.90 1.66 0.27 16.35<br />
5741 RB10299 455 1368 ATP-dependent carboligase related to biotin carboxylase (ORFY) 5563036 5564403 10 49949 + 6.45 1.68 0.30 17.52<br />
5771 RB10350 163 492 Thioredoxin 5596878 5596387 9.7 18371 - 6.23 1.55 0.09 5.81<br />
5931 RB10665 200 603 hypothetical protein 5789912 5790514 5.3 21401 + 6.30 1.70 0.20 11.68<br />
6053 RB10877 453 1362 membrane protein 5905128 5903767 4.3 48882 - 5.83 2.15 0.41 18.94<br />
6087 RB10936 56 171 hypothetical protein 5934348 5934518 12.5 6620 + 6.36 1.63 0.06 3.40<br />
6097 RB10954 143 432 hypothetical protein 5940749 5940318 10.4 16058 - 6.28 1.56 0.13 8.39<br />
6424 RB11504 72 219 conserved hypothetical protein 6221285 6221067 10.7 8212 - 6.78 2.65 0.08 3.00<br />
6430 RB11515 74 225 conserved hypothetical protein 6224420 6224644 11.7 8687 + 5.85 3.69 0.46 12.47<br />
6455 RB11566 195 588 conserved hypothetical protein 6243111 6243698 10.8 22135 + 6.40 1.91 0.20 10.23<br />
6633 RB11909 168 507 conserved hypothetical protein 6410157 6410663 4.1 18314 + 5.90 1.64 0.28 17.15<br />
6719 RB12067 107 324 conserved hypothetical protein 6510236 6510559 11.9 12169 + 6.31 1.50 0.13 8.55<br />
6815 RB12243 55 168 conserved hypothetical protein 6596256 6596423 10.5 5848 + 6.12 2.82 0.29 10.36<br />
7149 RB12830 102 309 conserved hypothetical protein 6920508 6920200 10.9 11753 - 6.17 2.07 0.13 6.49<br />
7160 RB12850 309 930 Rhodopirellula transposase 6926553 6925624 5.8 34461 - 6.12 1.81 0.29 15.86<br />
7161 RB12851 109 330 conserved hypothetical protein 6926556 6926885 7.5 12033 + 5.89 1.68 0.32 19.20<br />
7202 RB12936 580 1743 conserved hypothetical protein 6965872 6964130 5.3 66248 - 6.48 2.24 0.08 3.40<br />
7351 RB13222 208 627 SOUL heme-binding protein 7094714 7094088 8.7 22867 - 6.02 4.96 0.30 6.07<br />
40min ID Locus AA Nuc Product Gene EC Start Stop IEP MW(kDa) Strand i r Stdev %<br />
5.58 1.49 0.17 11.43<br />
1961 RB3596 144 435 nitrogen fixation protein (NifU protein) 1868895 1869329 4.3 15958 + 6.33 1.99 0.16 7.85<br />
3221 RB5717 497 1494 soluble pyridine nucleotide transhydrogenase sthA 1.6.1.1 2985257 2983764 6 54129 - 5.69 1.63 0.10 5.97<br />
3943 RB6993 305 918 phosphoadenosine phosphosulfate reductase cysH 1.8.4.8 3720852 3721769 5.7 33945 + 6.00 1.69 0.13 7.80<br />
3541 RB6300 208 627 para-aminobenzoate synthase pabA 6.3.5.8 3311474 3312100 5.3 22866 + 6.10 1.90 0.20 10.51<br />
2514 RB4517 117 354 Transcriptional coactivator/pterin dehydratase 2315930 2316283 5.8 13059 + 5.93 1.85 0.22 12.05<br />
2727 RB4868 316 951 lipoic acid synthetase lipA 2.8.1.- 2496444 2497394 6.1 35232 + 5.61 1.81 0.15 8.44<br />
2892 RB5135 137 414 6-pyruvoyl tetrahydrobiopterin synthase. 4.2.3.12 2640266 2639853 5.5 15985 - 5.61 1.73 0.12 7.11<br />
3958 RB7020 510 1533 uroporphyrin-III C-methyltransferase 2.1.1.107 3735125 3733593 5.6 55227 - 6.13 2.19 0.09 3.98<br />
1034 RB1977 385 1158 mRNA 3'-end processing factor 1039488 1040645 6 42759 + 5.75 1.55 0.26 16.68<br />
2144 RB3886 200 603 Ribosome recycling factor frr 2008765 2009367 4.7 22199 + 5.71 1.64 0.10 6.19
5194 RB9305 360 1083 peptide chain release factor 1 prfA 4974620 4975702 4.9 40794 + 6.48 1.55 0.12 7.72<br />
456 RB838 253 762 transcriptional regulator, TetR family 441593 440832 5.7 28095 - 5.90 1.91 0.06 2.96<br />
981 RB1862 135 408 transcriptional regulator, GntR family 960630 961037 10.2 14884 + 5.63 1.85 0.19 10.13<br />
4878 RB8695 131 396 transcriptional regulator, GntR family 4630087 4629692 9.9 14673 - 5.87 1.55 0.14 9.06<br />
5571 RB9999 281 846 conserved hypothetical protein 5386828 5385983 4.6 29948 - 6.23 2.72 0.14 5.14<br />
7362 RB13241 167 504 RNA polymerase ECF-type sigma factor 7103064 7102561 8.8 19488 - 5.89 1.79 0.18 9.99<br />
4187 RB7437 239 720 LexA repressor lexA 3.4.21.88 3987953 3988672 9.8 25951 + 5.68 1.67 0.26 15.67<br />
382 RB704 96 291 Transposase IS3/IS911 378137 378427 10.1 11124 + 6.15 1.89 0.22 11.57<br />
522 RB957 195 588 Tyrosine recombinase xerD xerD 505476 504889 10.2 21971 - 5.31 1.64 0.09 5.63<br />
628 RB1189 96 291 Transposase IS3/IS911 606838 607128 10.1 11124 + 6.33 1.58 0.28 17.48<br />
2381 RB4299 96 291 Transposase IS3/IS911 2214533 2214823 9.9 11182 + 6.38 2.19 0.23 10.26<br />
2463 RB4433 162 489 Ferritin and Dps 2277208 2277696 4.3 17757 + 6.49 2.15 0.18 8.27<br />
3320 RB5888 96 291 Transposase IS3/IS911 3087636 3087346 10.1 11124 - 6.23 2.22 0.14 6.36<br />
3420 RB6070 201 606 HNH endonuclease family protein 3186811 3187416 9.9 23317 + 6.02 1.62 0.18 11.23<br />
5728 RB10271 158 477 ribonuclease H rnhA 3.1.26.4 5552547 5552071 9.1 17665 - 5.88 1.97 0.22 11.08<br />
6481 RB11618 552 1659 type I restriction-modification system, M subunit, 6263708 6265366 6.7 62726 + 5.65 2.25 0.15 6.74<br />
6558 RB11749 96 291 Transposase IS3/IS911 6328064 6328354 10.1 11124 + 6.10 2.92 0.26 8.91<br />
6559 RB11750 292 879 integrase 6328357 6329235 10 33354 + 6.29 1.62 0.12 7.51<br />
6577 RB11802 96 291 Transposase IS3/IS911 6361950 6362240 10.1 11124 + 5.81 1.73 0.19 11.04<br />
7098 RB12746 294 885 Formamidopyrimidine-DNA glycolase mutM 3.2.2.23 6869843 6870727 10.3 32447 + 5.93 1.91 0.03 1.60<br />
7203 RB12940 96 291 Transposase IS3/IS911 6966246 6966536 10.1 11124 + 6.21 2.44 0.20 8.20<br />
7150 RB12831 264 795 Glycosyltransferase 6921326 6920532 4.6 29501 - 6.47 2.10 0.21 9.88<br />
731 RB1359 629 1890 Protease do precursor degP 3.4.21.- 693488 695377 6.1 66729 + 6.61 1.81 0.21 11.43<br />
1197 RB2268 282 849 peptide methionine sulfoxide reductase msrB 1.8.4.6 1191389 1190541 9.9 31115 - 6.60 2.34 0.46 19.65<br />
2726 RB4867 210 633 SmpB RNA-binding protein SmpB 2496354 2495722 10.8 23762 - 6.20 2.52 0.04 1.50<br />
3812 RB6751 859 2580 chaperone ClpB clpB 3586111 3588690 5.2 95764 + 6.31 1.51 0.29 19.42<br />
5094 RB9105 704 2115 Heat shock protein Hsp70 dnaK 4868834 4866720 4.6 76038 - 7.04 1.82 0.16 8.89<br />
5713 RB10242 330 993 Curved DNA-binding protein cbpA 5536118 5535126 6.7 35177 - 6.69 1.59 0.33 20.42<br />
5911 RB10627 93 282 Chaperonin Cpn10 5769148 5769429 3.9 9966 + 7.37 1.51 0.20 13.52<br />
3648 RB6469 252 759 ABC transporter ATP-binding protein 3419515 3420273 5.6 27684 + 6.04 2.30 0.12 5.12<br />
4528 RB8029 245 738 oxidoreductase, short-chain dehydrogenase/reductase family 4292625 4291888 4.5 25817 - 5.59 1.54 0.22 14.45<br />
5616 RB10074 273 822 conserved hypothetical protein 5435071 5434250 8.6 29283 - 5.96 1.62 0.29 17.87<br />
3933 RB6973 278 837 tellurite resistance protein 3710584 3711420 5 30569 + 5.67 1.79 0.33 18.67<br />
2571 RB4619 481 1446 dehydrogenase 2363086 2364531 5.1 52511 + 6.43 1.73 0.29 16.59<br />
2896 RB5140 613 1842 hypothetical protein 2641937 2643778 5.5 67565 + 5.94 2.08 0.36 17.37<br />
3602 RB6395 778 2337 conserved hypothetical protein 3364879 3367215 4.6 85714 + 5.85 1.92 0.10 5.15<br />
4796 RB8533 196 591 protease I pfpI 3.2.-.- 4552172 4552762 4.8 21046 + 6.22 1.55 0.20 12.80<br />
6370 RB11438 54 165 conserved hypothetical protein 6197926 6197762 11.3 6209 - 6.44 2.55 0.43 17.08<br />
7369 RB13257 286 861 2-hydroxy-6-ketonona-2,4-dienedioic acid hydrolase bphD 3.7.1.- 7105590 7106450 4.6 32540 + 6.47 2.15 0.06 2.63<br />
1502 RB2774 82 249 conserved hypothetical protein 1443786 1444034 4.4 9450 + 6.06 1.94 0.19 9.96<br />
1985 RB3630 313 942 conserved hypothetical protein 1888468 1889409 6.3 34702 + 5.45 1.73 0.33 18.88<br />
2453 RB4419 178 537 MgtC/SapB transporter 2272912 2273448 6.2 18995 + 6.60 1.79 0.18 9.82<br />
4776 RB8505 439 1320 serine/threonine protein kinase related protein 4538446 4537127 4.7 48224 - 6.00 1.53 0.10 6.56<br />
3294 RB5848 140 423 dnaK suppressor protein, 3066328 3065906 4.6 15633 - 6.87 1.84 0.24 13.31<br />
6266 RB11260 121 366 dnaK suppressor protein, 6107202 6106837 5.2 13639 - 6.53 1.53 0.08 5.19<br />
10 RB17 164 495 protein containing DUF1589 8251 7757 11.5 18498 - 5.72 2.49 0.25 10.09<br />
79 RB138 214 645 Sigma factor, ECF-like 60872 61516 4.8 24057 + 6.37 2.18 0.26 12.06<br />
173 RB303 53 162 hypothetical protein 151630 151791 11.5 5832 + 6.21 1.54 0.23 15.15<br />
234 RB402 151 456 hypothetical protein 212820 213275 4.4 17306 + 5.62 2.33 0.23 9.74<br />
299 RB519 54 165 hypothetical protein 285036 284872 10.6 6171 - 5.20 1.73 0.28 16.00<br />
301 RB521 63 192 hypothetical protein 286399 286208 10.7 7040 - 5.45 1.70 0.11 6.37<br />
319 RB550 137 414 secreted protein 306709 306296 12.2 15112 - 5.89 2.32 0.12 5.13<br />
362 RB663 79 240 hypothetical protein 357608 357369 11.4 8792 - 6.14 1.69 0.23 13.44<br />
475 RB873 74 225 hypothetical protein 463836 464060 9.6 8292 + 5.82 1.77 0.15 8.66<br />
506 RB933 208 627 hypothetical protein 493451 492825 12.2 22575 - 5.22 2.10 0.27 12.89<br />
507 RB934 375 1128 Putative transposase 494203 493076 9.9 41881 - 5.76 2.21 0.22 10.05<br />
520 RB955 208 627 hypothetical protein 504140 503514 12.2 22575 - 5.72 2.49 0.07 2.95<br />
556 RB1043 89 270 conserved hypothetical protein 543083 543352 4.7 10039 + 6.69 1.52 0.21 13.97<br />
624 RB1182 88 267 hypothetical protein 604706 604972 11.6 9996 + 5.97 1.55 0.14 9.24<br />
669 RB1255 51 156 hypothetical protein 640910 640755 12.3 5933 - 5.36 1.96 0.01 0.75<br />
670 RB1256 51 156 hypothetical protein 641044 640889 12 5840 - 5.23 1.64 0.36 22.15<br />
774 RB1449 65 198 hypothetical protein 736500 736697 10 7487 + 5.56 1.66 0.16 9.65<br />
947 RB1789 243 732 conserved hypothetical protein 925861 926592 9.4 27028 + 5.99 1.52 0.06 4.06<br />
985 RB1872 38 117 hypothetical protein 966053 966169 12.3 4345 + 5.97 1.69 0.15 9.14<br />
1044 RB1999 37 114 hypothetical protein 1053955 1054068 12.2 4228 + 6.14 1.78 0.42 23.36<br />
1150 RB2185 50 153 hypothetical protein 1152681 1152529 10.1 5841 - 5.64 1.92 0.31 16.11<br />
1435 RB2649 53 162 hypothetical protein 1374987 1375148 9.1 5813 + 6.48 1.85 0.26 13.86<br />
1477 RB2724 266 801 membrane protein 1419817 1419017 7.4 29523 - 6.37 2.10 0.21 10.24<br />
1490 RB2745 41 126 hypothetical protein 1431275 1431400 9.9 4387 + 5.99 1.58 0.35 22.34<br />
1518 RB2809 226 681 hypothetical protein 1459745 1459065 9.1 25318 - 5.91 2.59 0.36 13.93<br />
1566 RB2900 90 273 hypothetical protein 1506927 1507199 9.1 9939 + 6.46 1.64 0.31 18.94<br />
1606 RB2963 119 360 hypothetical protein 1526707 1527066 12.2 13440 + 6.00 1.96 0.10 5.18<br />
1640 RB3020 99 300 membrane protein 1566234 1565935 9.8 10462 - 6.15 1.57 0.18 11.58<br />
1711 RB3159 73 222 protein containing DUF1590 1629337 1629116 12.1 8119 - 6.78 1.53 0.08 5.54<br />
1728 RB3196 109 330 hypothetical protein 1650093 1650422 10.1 12287 + 6.48 2.87 0.20 7.09<br />
1868 RB3430 248 747 conserved hypothetical protein, membrane 1772646 1773392 7.5 26767 + 6.31 1.70 0.31 18.33<br />
1957 RB3587 113 342 hypothetical protein 1864796 1864455 11.8 13003 - 5.83 1.55 0.31 19.87<br />
2169 RB3929 54 165 hypothetical protein 2034407 2034243 10.7 5796 - 5.28 2.41 0.32 13.16<br />
2170 RB3930 54 165 hypothetical protein 2034417 2034253 11.6 6445 - 5.43 1.50 0.27 18.30<br />
2330 RB4219 74 225 hypothetical protein 2163305 2163081 6.8 8050 - 5.59 2.05 0.33 16.13<br />
2440 RB4397 55 168 protein containing DUF1560 2263976 2264143 10.5 5816 + 6.24 1.69 0.39 23.18<br />
2443 RB4400 58 177 hypothetical protein 2265382 2265206 9 6610 - 6.12 2.05 0.16 7.77<br />
2458 RB4426 60 183 hypothetical protein 2275786 2275604 6.3 6516 - 5.71 2.12 0.23 10.70<br />
2465 RB4438 160 483 Pyridoxamine 5'-phosphate oxidase- 2278247 2278729 4.4 18043 + 6.45 2.52 0.07 2.79<br />
2584 RB4642 85 258 conserved hypothetical protein 2377301 2377558 4.4 9855 + 6.35 2.35 0.09 3.66<br />
2585 RB4643 96 291 conserved hypothetical protein 2377555 2377845 8.9 11064 + 5.92 2.37 0.14 6.00<br />
2608 RB4678 199 600 secreted protein 2390618 2391217 4.2 20927 + 6.64 1.91 0.10 5.11<br />
2609 RB4679 175 528 secreted protein 2391214 2391741 4.2 18596 + 6.74 1.60 0.36 22.49<br />
2854 RB5069 65 198 hypothetical protein 2609575 2609378 10.3 7107 - 5.36 2.02 0.39 19.40<br />
2866 RB5092 59 180 secreted protein 2618771 2618950 3.7 6013 + 5.58 1.73 0.08 4.40<br />
2881 RB5114 38 117 hypothetical protein 2625052 2624936 10.8 4310 - 5.54 1.92 0.17 8.87<br />
2951 RB5238 73 222 hypothetical protein 2697775 2697996 10.6 7866 + 5.92 2.66 0.11 4.11<br />
3022 RB5370 309 930 Rhodopirellula transposase 2774826 2773897 5.9 34530 - 5.98 1.60 0.21 13.31<br />
3023 RB5371 109 330 hypothetical protein 2774829 2775158 7.5 12033 + 5.45 1.62 0.10 6.15<br />
3047 RB5412 72 219 hypothetical protein 2794838 2794620 12.3 8592 - 6.09 1.58 0.05 2.95<br />
3118 RB5523 91 276 hypothetical protein 2860984 2860709 6.3 9939 - 5.98 2.19 0.13 5.81<br />
3130 RB5551 663 1992 hypothetical protein 2889101 2891092 5.7 70932 + 5.94 2.31 0.03 1.36<br />
3139 RB5567 66 201 hypothetical protein 2897766 2897566 12.1 7106 - 6.03 2.84 0.42 14.69<br />
3295 RB5850 63 192 hypothetical protein 3066498 3066307 5.8 7096 - 6.34 1.70 0.42 24.80<br />
3346 RB5938 370 1113 hypothetical protein 3113484 3112372 5.6 41837 - 5.85 2.57 0.20 7.78<br />
3355 RB5955 177 534 hypothetical protein 3121488 3122021 4.5 19364 + 6.25 1.67 0.18 10.96<br />
3363 RB5968 207 624 hypothetical protein 3128602 3129225 9.7 23009 + 5.33 1.68 0.31 18.29<br />
3390 RB6011 50 153 hypothetical protein 3150235 3150083 10.1 5841 - 5.74 1.96 0.28 14.07<br />
3391 RB6012 433 1302 ISXo8 transposase 3151547 3150246 9.4 49646 - 5.11 2.31 0.34 14.72<br />
3457 RB6144 38 117 hypothetical protein 3228305 3228189 11.1 4314 - 6.17 2.94 0.42 14.16<br />
3471 RB6167 375 1128 Putative transposase 3235937 3237064 9.9 41895 + 5.92 1.74 0.16 9.00<br />
3472 RB6168 208 627 hypothetical protein 3236689 3237315 12.2 22575 + 6.05 2.17 0.31 14.32<br />
3482 RB6188 68 207 hypothetical protein 3244384 3244178 9.6 6986 - 6.17 1.73 0.30 17.51<br />
3531 RB6282 200 603 conserved hypothetical protein 3302795 3303397 4.8 22451 + 6.19 2.22 0.42 19.02<br />
3588 RB6379 153 462 hypothetical protein 3353122 3352661 5.8 17398 - 6.75 1.50 0.11 7.16<br />
3739 RB6627 176 531 hypothetical protein 3522169 3522699 6 19706 + 6.14 1.63 0.26 15.98<br />
3741 RB6629 708 2127 secreted protein 3522859 3524985 5.1 78383 + 5.59 1.69 0.18 10.49<br />
3745 RB6636 433 1302 ISXo8 transposase 3527005 3528306 9.4 49646 + 5.60 1.65 0.29 17.80<br />
3746 RB6637 50 153 hypothetical protein 3528317 3528469 10.1 5841 + 5.45 1.63 0.20 12.23<br />
3771 RB6685 72 219 hypothetical protein 3554265 3554047 12.2 8077 - 5.85 1.84 0.23 12.45<br />
3784 RB6706 37 114 hypothetical protein 3560942 3560829 7.3 4375 - 5.79 1.94 0.14 6.99<br />
3804 RB6736 433 1302 ISXo8 transposase 3578852 3577551 9.4 49646 - 5.92 2.13 0.34 16.10<br />
3888 RB6888 420 1263 secreted protein containing DUF1573 3662449 3661187 4.3 45773 - 6.09 2.92 0.49 16.85<br />
3969 RB7039 202 609 hypothetical protein 3743573 3742965 7.9 23105 - 5.43 1.87 0.37 20.04<br />
3981 RB7063 148 447 hypothetical protein 3751250 3751696 10.5 17004 + 6.38 2.98 0.19 6.25<br />
4157 RB7389 375 1128 Putative transposase 3953590 3954717 9.9 41895 + 5.70 2.58 0.35 13.59<br />
4161 RB7394 136 411 hypothetical protein 3955937 3955527 4.2 14568 - 6.19 1.52 0.18 11.97<br />
4291 RB7612 136 411 hypothetical protein 4092907 4093317 11.4 15607 + 5.91 2.82 0.16 5.50
4326 RB7666 72 219 hypothetical protein 4116984 4116766 3.1 7265 - 6.50 1.73 0.26 14.73<br />
4497 RB7960 363 1092 ATP:guanido phosphotransferase 2.7.3.- 4257723 4256632 5.4 41235 - 6.29 1.80 0.38 21.30<br />
4499 RB7965 53 162 hypothetical protein 4258303 4258464 7.4 5879 + 6.79 1.69 0.07 4.02<br />
4681 RB8325 296 891 hypothetical protein 4447029 4446139 6.8 32118 - 5.58 2.36 0.06 2.48<br />
4704 RB8365 90 273 membrane protein 4463903 4463631 11.7 9405 - 6.39 2.01 0.12 6.21<br />
4714 RB8384 433 1302 ISXo8 transposase 4478623 4479924 9.4 49646 + 5.78 2.10 0.21 10.20<br />
4797 RB8534 42 129 hypothetical protein 4552802 4552930 12.2 4909 + 5.11 1.79 0.27 15.12<br />
5038 RB8987 48 147 hypothetical protein 4790856 4790710 9.1 5309 - 5.65 2.40 0.21 8.88<br />
5055 RB9024 46 141 hypothetical protein 4809190 4809050 10.3 5125 - 5.80 1.71 0.17 9.69<br />
5157 RB9230 107 324 hypothetical protein 4936673 4936996 9.9 11900 + 6.11 2.13 0.18 8.31<br />
5473 RB9811 102 309 secreted protein 5284365 5284673 11.7 11169 + 5.77 1.61 0.32 19.58<br />
5524 RB9907 433 1302 ISXo8 transposase 5338185 5339486 9.4 49646 + 6.00 1.92 0.10 5.15<br />
5525 RB9908 50 153 hypothetical protein 5339497 5339649 9.9 5867 + 5.43 1.80 0.31 17.49<br />
5565 RB9986 112 339 hypothetical protein 5380717 5380379 11.1 12671 - 5.77 2.34 0.07 2.89<br />
5654 RB10137 73 222 protein containing DUF1590 5469772 5469551 12.1 8049 - 5.77 2.03 0.15 7.46<br />
5874 RB10556 134 405 conserved hypothetical protein, membrane 5727064 5727468 10.5 15094 + 5.73 1.50 0.22 14.88<br />
5910 RB10623 60 183 secreted protein 5769140 5768958 3.9 6284 - 7.96 1.56 0.26 16.87<br />
5931 RB10665 200 603 hypothetical protein 5789912 5790514 5.3 21401 + 6.37 1.95 0.23 11.68<br />
6053 RB10877 453 1362 membrane protein 5905128 5903767 4.3 48882 - 5.40 1.60 0.18 11.36<br />
6087 RB10936 56 171 hypothetical protein 5934348 5934518 12.5 6620 + 6.21 1.78 0.44 24.60<br />
6097 RB10954 143 432 hypothetical protein 5940749 5940318 10.4 16058 - 5.90 1.70 0.23 13.72<br />
6361 RB11421 552 1659 60-kDa SS-A/Ro ribonucleoprotein rsr 6195538 6193880 9.6 59999 - 5.87 2.55 0.12 4.74<br />
6382 RB11446 113 342 conserved hypothetical protein 6199492 6199833 10 12579 + 7.28 1.79 0.07 3.88<br />
6406 RB11475 57 174 conserved hypothetical protein 6209468 6209641 4.7 5927 + 6.11 1.72 0.08 4.65<br />
6455 RB11566 195 588 conserved hypothetical protein 6243111 6243698 10.8 22135 + 6.44 2.20 0.20 9.25<br />
6472 RB11603 37 114 conserved hypothetical protein 6256722 6256835 9 3998 + 7.07 1.76 0.20 11.32<br />
6519 RB11675 172 519 hypothetical protein 6299494 6300012 5.1 18614 + 6.09 1.56 0.16 10.36<br />
6606 RB11855 101 306 conserved hypothetical protein 6383765 6383460 12.5 11384 - 5.75 1.57 0.14 8.94<br />
6637 RB11918 134 405 protein containing DUF971 6415701 6415297 6.1 14675 - 5.96 1.56 0.28 17.73<br />
6661 RB11952 131 396 secreted protein 6438438 6438043 9.5 14509 - 5.52 2.70 0.30 11.05<br />
6719 RB12067 107 324 conserved hypothetical protein 6510236 6510559 11.9 12169 + 6.24 2.49 0.34 13.71<br />
6798 RB12215 169 510 conserved hypothetical protein 6584611 6584102 10.7 18687 - 5.83 2.21 0.03 1.31<br />
6815 RB12243 55 168 conserved hypothetical protein 6596256 6596423 10.5 5848 + 5.75 1.74 0.23 13.21<br />
6878 RB12348 260 783 FxsA cytoplasmic membrane protein 6645520 6644738 11.6 28277 - 6.86 1.73 0.16 9.24<br />
6897 RB12387 50 153 conserved hypothetical protein 6665894 6665742 10.1 5841 - 5.50 1.91 0.38 19.75<br />
6944 RB12458 85 258 conserved hypothetical protein 6704719 6704976 10.9 8948 + 6.08 2.67 0.34 12.78<br />
6950 RB12471 85 258 conserved hypothetical protein 6712442 6712185 9.7 9841 - 6.30 1.50 0.27 18.27<br />
7133 RB12808 369 1110 secreted protein 6912350 6911241 4.6 40963 - 5.83 2.14 0.22 10.26<br />
7149 RB12830 102 309 conserved hypothetical protein 6920508 6920200 10.9 11753 - 6.08 2.16 0.22 10.21<br />
7160 RB12850 309 930 Rhodopirellula transposase 6926553 6925624 5.8 34461 - 5.90 2.07 0.14 6.79<br />
7161 RB12851 109 330 conserved hypothetical protein 6926556 6926885 7.5 12033 + 5.66 1.86 0.18 9.60<br />
7202 RB12936 580 1743 conserved hypothetical protein 6965872 6964130 5.3 66248 - 6.21 1.75 0.11 6.18<br />
7256 RB13053 142 429 secreted protein 7008596 7009024 5.6 14910 + 6.09 1.64 0.28 16.92<br />
7278 RB13093 158 477 secreted protein 7025577 7025101 9.6 17298 - 6.01 2.81 0.39 14.01<br />
7280 RB13095 229 690 conserved hypothetical protein 7026753 7027442 7.7 24080 + 6.24 1.88 0.28 14.75<br />
7281 RB13097 171 516 membrane protein 7027310 7026795 4.5 17420 - 5.49 1.62 0.19 11.92<br />
60min<br />
ID Locus AA Nuc Product Gene EC Start Stop IEP MW(kDa) Strand i r Stdev %<br />
1961 RB3596 144 435 nitrogen fixation protein (NifU protein) 1868895 1869329 4.3 15958 + 6.25 2.03 0.20 9.91<br />
3221 RB5717 497 1494 soluble pyridine nucleotide transhydrogenase sthA 1.6.1.1 2985257 2983764 6 54129 - 6.29 1.79 0.11 6.41<br />
1319 RB2464 856 2571 tyrosine-protein kinase-like protein 1287262 1284692 5.2 93341 - 6.24 1.88 0.35 18.82<br />
6638 RB11919 671 2016 Dihydroxy-acid and 6-phosphogluconate dehydratase 6417713 6415698 5.3 70685 - 5.96 2.54 0.59 23.38<br />
3541 RB6300 208 627 para-aminobenzoate synthase pabA 6.3.5.8 3311474 3312100 5.3 22866 + 6.17 1.55 0.28 17.82<br />
5972 RB10733 389 1170 Glutathione-dependent formaldehyde dehydrogenase fdh 1.2.1.1 5832679 5833848 4.8 42303 + 6.26 1.64 0.22 13.35<br />
2891 RB5134 208 627 Ham1-like protein 2639251 2639877 5.7 22783 + 6.08 2.54 0.34 13.25<br />
2930 RB5200 942 2829 alpha amylase 2681425 2684253 4.6 105281 + 6.15 1.60 0.17 10.40<br />
2514 RB4517 117 354 Transcriptional coactivator/pterin dehydratase 2315930 2316283 5.8 13059 + 6.23 1.61 0.14 8.51<br />
2727 RB4868 316 951 lipoic acid synthetase lipA 2.8.1.- 2496444 2497394 6.1 35232 + 6.03 1.50 0.06 4.02<br />
2892 RB5135 137 414 6-pyruvoyl tetrahydrobiopterin synthase. 4.2.3.12 2640266 2639853 5.5 15985 - 6.10 2.19 0.39 17.98<br />
1034 RB1977 385 1158 mRNA 3'-end processing factor 1039488 1040645 6 42759 + 5.98 2.05 0.37 18.02<br />
5571 RB9999 281 846 conserved hypothetical protein 5386828 5385983 4.6 29948 - 6.23 2.41 0.15 6.28<br />
5606 RB10049 217 654 RNA polymerase ECF-type sigma factor 5419507 5420160 10.1 25205 + 6.48 1.80 0.42 23.27<br />
4187 RB7437 239 720 LexA repressor lexA 3.4.21.88 3987953 3988672 9.8 25951 + 6.02 2.11 0.46 21.90<br />
98 RB170 96 291 Transposase IS3/IS911 89333 89623 10.1 11124 + 6.35 2.58 0.03 1.32<br />
99 RB171 292 879 ISxac3 transposase 89626 90504 10 33354 + 6.17 1.82 0.21 11.63<br />
382 RB704 96 291 Transposase IS3/IS911 378137 378427 10.1 11124 + 6.49 1.73 0.29 16.68<br />
522 RB957 195 588 Tyrosine recombinase xerD xerD 505476 504889 10.2 21971 - 5.84 2.07 0.20 9.52<br />
1127 RB2146 139 420 Exonuclease VII, small subunit xseB 3.1.11.6 1136505 1136086 4.2 14778 - 6.69 2.06 0.35 17.06<br />
2463 RB4433 162 489 Ferritin and Dps 2277208 2277696 4.3 17757 + 7.12 2.15 0.39 18.16<br />
2695 RB4811 438 1317 ISPg4, transposase 2465768 2467084 9.3 48850 + 5.62 2.21 0.39 17.83<br />
2815 RB4998 207 624 3-methyladenine DNA glycosylase 2572250 2571627 7.5 23370 - 5.59 1.54 0.14 8.98<br />
3320 RB5888 96 291 Transposase IS3/IS911 3087636 3087346 10.1 11124 - 6.39 2.23 0.54 24.20<br />
5472 RB9810 438 1317 transposase of IS5377-like element 5283122 5284438 9.3 48850 + 5.61 1.64 0.32 19.39<br />
6481 RB11618 552 1659 type I restriction-modification system, M subunit, 6263708 6265366 6.7 62726 + 5.80 2.30 0.51 22.00<br />
6558 RB11749 96 291 Transposase IS3/IS911 6328064 6328354 10.1 11124 + 6.39 2.56 0.45 17.56<br />
6577 RB11802 96 291 Transposase IS3/IS911 6361950 6362240 10.1 11124 + 6.23 1.75 0.24 13.93<br />
7098 RB12746 294 885 Formamidopyrimidine-DNA glycolase mutM 3.2.2.23 6869843 6870727 10.3 32447 + 6.33 2.07 0.31 14.98<br />
7203 RB12940 96 291 Transposase IS3/IS911 6966246 6966536 10.1 11124 + 6.48 2.31 0.52 22.74<br />
7150 RB12831 264 795 Glycosyltransferase 6921326 6920532 4.6 29501 - 6.82 1.53 0.29 19.22<br />
2171 RB3932 257 774 MotA/TolQ/ExbB proton channel 2034427 2035200 4.2 26838 + 6.05 2.36 0.05 1.99<br />
6532 RB11690 1238 3717 preprotein translocase SecA subunit secA 6306704 6302988 4.9 139847 - 6.16 1.54 0.21 13.81<br />
1197 RB2268 282 849 peptide methionine sulfoxide reductase msrB 1.8.4.6 1191389 1190541 9.9 31115 - 6.86 2.01 0.30 14.84<br />
2726 RB4867 210 633 SmpB RNA-binding protein SmpB 2496354 2495722 10.8 23762 - 6.33 2.58 0.41 15.87<br />
3812 RB6751 859 2580 chaperone ClpB clpB 3586111 3588690 5.2 95764 + 7.09 1.57 0.09 6.01<br />
5094 RB9105 704 2115 Heat shock protein Hsp70 dnaK 4868834 4866720 4.6 76038 - 7.48 2.12 0.46 21.53<br />
212 RB370 553 1662 nitrate transporter substrate-binding protein 184428 186089 4.6 60742 + 6.07 1.76 0.37 21.30<br />
3933 RB6973 278 837 tellurite resistance protein 3710584 3711420 5 30569 + 6.21 2.07 0.13 6.25<br />
2896 RB5140 613 1842 hypothetical protein 2641937 2643778 5.5 67565 + 6.27 1.56 0.17 11.13<br />
3602 RB6395 778 2337 conserved hypothetical protein 3364879 3367215 4.6 85714 + 6.28 2.57 0.22 8.65<br />
3936 RB6982 286 861 ABC transporter ATP-binding protein 3715019 3715879 5 31150 + 6.09 2.50 0.57 22.72<br />
7369 RB13257 286 861 2-hydroxy-6-ketonona-2,4-dienedioic acid hydrolase bphD 3.7.1.- 7105590 7106450 4.6 32540 + 6.56 2.04 0.19 9.19<br />
1502 RB2774 82 249 conserved hypothetical protein 1443786 1444034 4.4 9450 + 6.71 1.51 0.27 17.83<br />
1985 RB3630 313 942 conserved hypothetical protein 1888468 1889409 6.3 34702 + 5.78 1.58 0.31 19.72<br />
3490 RB6207 436 1311 conserved hypothetical protein, secreted 3252449 3253759 4.8 48106 + 6.60 1.59 0.20 12.82<br />
4294 RB7616 221 666 protein containing DUF162 4094752 4094087 4.7 23976 - 6.12 2.19 0.13 5.99<br />
4912 RB8763 357 1074 conserved hypothetical protein, membrane 4658536 4657463 4.7 39980 - 5.84 1.65 0.14 8.27<br />
5032 RB8976 92 279 conserved hypothetical protein 4783568 4783846 9.8 10362 + 6.00 3.55 0.10 2.70<br />
3294 RB5848 140 423 dnaK suppressor protein, 3066328 3065906 4.6 15633 - 7.18 1.79 0.25 13.74<br />
10 RB17 164 495 protein containing DUF1589 8251 7757 11.5 18498 - 6.16 2.46 0.46 18.67<br />
79 RB138 214 645 Sigma factor, ECF-like 60872 61516 4.8 24057 + 6.61 1.98 0.21 10.74<br />
118 RB202 50 153 hypothetical protein 107884 107732 11.9 5236 - 5.77 1.59 0.38 23.95<br />
163 RB286 105 318 hypothetical protein 142779 143096 11.6 12056 + 5.78 2.14 0.32 14.86<br />
234 RB402 151 456 hypothetical protein 212820 213275 4.4 17306 + 5.91 2.63 0.25 9.43<br />
299 RB519 54 165 hypothetical protein 285036 284872 10.6 6171 - 5.98 3.64 0.85 23.31<br />
300 RB520 375 1128 conserved hypothetical protein 284989 286116 4.8 41300 + 6.60 2.04 0.14 7.03<br />
301 RB521 63 192 hypothetical protein 286399 286208 10.7 7040 - 6.61 3.68 0.49 13.17<br />
316 RB546 485 1458 secreted protein containing DUF1501 302834 304291 7.8 53385 + 6.00 1.94 0.48 24.52<br />
319 RB550 137 414 secreted protein 306709 306296 12.2 15112 - 6.36 2.16 0.09 4.20<br />
500 RB924 208 627 hypothetical protein 490400 489774 12.2 22548 - 5.80 2.52 0.33 12.98<br />
506 RB933 208 627 hypothetical protein 493451 492825 12.2 22575 - 5.55 1.94 0.21 10.78<br />
507 RB934 375 1128 Putative transposase 494203 493076 9.9 41881 - 6.10 2.24 0.08 3.65<br />
520 RB955 208 627 hypothetical protein 504140 503514 12.2 22575 - 6.06 2.50 0.35 13.83<br />
596 RB1133 63 192 hypothetical protein 583629 583438 11.3 6841 - 5.60 1.89 0.45 23.69<br />
657 RB1232 41 126 hypothetical protein 628776 628901 12.4 4869 + 6.17 1.59 0.19 12.01<br />
667 RB1252 665 1998 secreted protein 638429 640426 3.8 72471 + 5.72 1.83 0.22 11.88<br />
669 RB1255 51 156 hypothetical protein 640910 640755 12.3 5933 - 6.03 3.22 0.44 13.69<br />
670 RB1256 51 156 hypothetical protein 641044 640889 12 5840 - 5.77 2.66 0.56 20.95<br />
774 RB1449 65 198 hypothetical protein 736500 736697 10 7487 + 5.87 1.91 0.20 10.31<br />
844 RB1573 36 111 hypothetical protein 805094 804984 11.9 4165 - 6.02 2.22 0.18 7.97<br />
913 RB1722 651 1956 hypothetical protein 897688 899643 5.4 70377 + 6.51 2.08 0.37 17.91<br />
951 RB1796 52 159 hypothetical protein 930376 930534 11.3 5841 + 5.99 1.51 0.18 11.92<br />
963 RB1830 37 114 hypothetical protein 945611 945498 6.6 3673 - 6.08 1.60 0.19 11.96
1150 RB2185 50 153 hypothetical protein 1152681 1152529 10.1 5841 - 6.04 2.61 0.05 2.07<br />
1151 RB2186 433 1302 ISXo8 transposase 1153993 1152692 9.4 49646 - 6.01 1.86 0.21 11.49<br />
1320 RB2465 52 159 hypothetical protein 1287232 1287390 10 5687 + 6.17 1.68 0.35 20.62<br />
1435 RB2649 53 162 hypothetical protein 1374987 1375148 9.1 5813 + 6.45 1.68 0.06 3.70<br />
1461 RB2697 508 1527 protein containing DUF1501 1402745 1404271 6.9 56235 + 6.17 1.92 0.18 9.23<br />
1490 RB2745 41 126 hypothetical protein 1431275 1431400 9.9 4387 + 6.13 1.57 0.28 17.96<br />
1518 RB2809 226 681 hypothetical protein 1459745 1459065 9.1 25318 - 6.37 2.39 0.12 4.91<br />
1606 RB2963 119 360 hypothetical protein 1526707 1527066 12.2 13440 + 6.41 1.79 0.23 12.81<br />
1728 RB3196 109 330 hypothetical protein 1650093 1650422 10.1 12287 + 6.59 2.62 0.33 12.51<br />
1868 RB3430 248 747 conserved hypothetical protein, membrane 1772646 1773392 7.5 26767 + 6.43 1.56 0.10 6.39<br />
2169 RB3929 54 165 hypothetical protein 2034407 2034243 10.7 5796 - 5.81 2.79 0.22 7.74<br />
2170 RB3930 54 165 hypothetical protein 2034417 2034253 11.6 6445 - 5.93 2.19 0.14 6.58<br />
2304 RB4176 96 291 hypothetical protein 2146928 2146638 12.4 11402 - 6.61 1.56 0.05 3.39<br />
2443 RB4400 58 177 hypothetical protein 2265382 2265206 9 6610 - 6.33 2.21 0.29 12.97<br />
2465 RB4438 160 483 Pyridoxamine 5'-phosphate oxidase- 2278247 2278729 4.4 18043 + 6.68 2.37 0.37 15.77<br />
2608 RB4678 199 600 secreted protein 2390618 2391217 4.2 20927 + 6.84 1.80 0.32 17.86<br />
2609 RB4679 175 528 secreted protein 2391214 2391741 4.2 18596 + 7.15 1.56 0.35 22.36<br />
2617 RB4698 86 261 hypothetical protein 2397601 2397861 12.2 9541 + 6.01 2.15 0.20 9.27<br />
2696 RB4812 92 279 secreted protein 2467011 2467289 12.5 10014 + 5.75 1.89 0.24 12.85<br />
2854 RB5069 65 198 hypothetical protein 2609575 2609378 10.3 7107 - 5.68 2.06 0.16 7.62<br />
2866 RB5092 59 180 secreted protein 2618771 2618950 3.7 6013 + 6.26 2.77 0.44 16.02<br />
2881 RB5114 38 117 hypothetical protein 2625052 2624936 10.8 4310 - 5.91 1.61 0.38 23.49<br />
2951 RB5238 73 222 hypothetical protein 2697775 2697996 10.6 7866 + 6.50 2.71 0.21 7.73<br />
3006 RB5340 968 2907 conserved hypothetical protein 2757894 2760800 4.8 108024 + 5.80 1.52 0.32 21.23<br />
3023 RB5371 109 330 hypothetical protein 2774829 2775158 7.5 12033 + 5.99 1.76 0.28 15.76<br />
3118 RB5523 91 276 hypothetical protein 2860984 2860709 6.3 9939 - 6.34 2.00 0.35 17.41<br />
3130 RB5551 663 1992 hypothetical protein 2889101 2891092 5.7 70932 + 6.16 1.82 0.30 16.52<br />
3139 RB5567 66 201 hypothetical protein 2897766 2897566 12.1 7106 - 6.43 2.55 0.39 15.25<br />
3295 RB5850 63 192 hypothetical protein 3066498 3066307 5.8 7096 - 6.80 1.71 0.32 18.45<br />
3390 RB6011 50 153 hypothetical protein 3150235 3150083 10.1 5841 - 6.25 2.25 0.18 8.17<br />
3391 RB6012 433 1302 ISXo8 transposase 3151547 3150246 9.4 49646 - 5.46 2.00 0.49 24.76<br />
3457 RB6144 38 117 hypothetical protein 3228305 3228189 11.1 4314 - 6.45 2.16 0.17 7.83<br />
3472 RB6168 208 627 hypothetical protein 3236689 3237315 12.2 22575 + 6.46 1.68 0.39 23.26<br />
3746 RB6637 50 153 hypothetical protein 3528317 3528469 10.1 5841 + 6.03 2.34 0.27 11.55<br />
3771 RB6685 72 219 hypothetical protein 3554265 3554047 12.2 8077 - 6.37 1.95 0.48 24.71<br />
3784 RB6706 37 114 hypothetical protein 3560942 3560829 7.3 4375 - 6.54 1.78 0.28 15.67<br />
3803 RB6735 50 153 hypothetical protein 3577540 3577388 9.9 5867 - 5.81 2.12 0.46 21.57<br />
3804 RB6736 433 1302 ISXo8 transposase 3578852 3577551 9.4 49646 - 6.19 1.82 0.26 14.07<br />
3888 RB6888 420 1263 secreted protein containing DUF1573 3662449 3661187 4.3 45773 - 6.42 2.56 0.45 17.57<br />
4151 RB7381 243 732 membrane protein 3950326 3949595 7.4 26253 - 6.55 2.54 0.62 24.39<br />
4225 RB7507 521 1566 conserved hypothetical protein, membrane 4036748 4038313 5.1 57952 + 5.98 1.73 0.07 4.12<br />
4291 RB7612 136 411 hypothetical protein 4092907 4093317 11.4 15607 + 5.97 1.89 0.31 16.55<br />
4499 RB7965 53 162 hypothetical protein 4258303 4258464 7.4 5879 + 6.72 1.51 0.09 6.07<br />
4595 RB8153 486 1461 conserved hypothetical protein 4359392 4357932 4.8 54199 - 6.00 2.25 0.18 8.16<br />
4704 RB8365 90 273 membrane protein 4463903 4463631 11.7 9405 - 6.74 1.91 0.47 24.54<br />
4714 RB8384 433 1302 ISXo8 transposase 4478623 4479924 9.4 49646 + 6.05 1.79 0.44 24.83<br />
4715 RB8385 50 153 hypothetical protein 4479935 4480087 10.1 5841 + 6.13 1.60 0.13 7.98<br />
4761 RB8478 141 426 hypothetical protein 4524759 4524334 11.6 15805 - 5.96 1.50 0.37 24.70<br />
5027 RB8969 101 306 Chaperonin Cpn10 4779554 4779859 4.9 10628 + 7.34 2.03 0.36 17.98<br />
5038 RB8987 48 147 hypothetical protein 4790856 4790710 9.1 5309 - 6.17 3.00 0.61 20.17<br />
5157 RB9230 107 324 hypothetical protein 4936673 4936996 9.9 11900 + 6.44 2.07 0.05 2.40<br />
5456 RB9787 61 186 hypothetical protein 5270904 5270719 6.1 6701 - 6.32 3.54 0.71 19.96<br />
5473 RB9811 102 309 secreted protein 5284365 5284673 11.7 11169 + 6.25 1.76 0.09 5.00<br />
5524 RB9907 433 1302 ISXo8 transposase 5338185 5339486 9.4 49646 + 6.33 1.70 0.39 22.73<br />
5525 RB9908 50 153 hypothetical protein 5339497 5339649 9.9 5867 + 6.00 2.42 0.11 4.46<br />
5858 RB10531 43 132 protein containing DUF1586 5712827 5712958 10.6 4745 + 6.13 1.97 0.18 8.92<br />
5910 RB10623 60 183 secreted protein 5769140 5768958 3.9 6284 - 8.35 1.56 0.39 24.99<br />
5931 RB10665 200 603 hypothetical protein 5789912 5790514 5.3 21401 + 6.30 2.35 0.34 14.51<br />
5968 RB10728 132 399 secreted protein 5831649 5832047 9.9 13833 + 7.12 1.68 0.39 23.06<br />
6053 RB10877 453 1362 membrane protein 5905128 5903767 4.3 48882 - 6.03 2.35 0.48 20.41<br />
6361 RB11421 552 1659 60-kDa SS-A/Ro ribonucleoprotein rsr 6195538 6193880 9.6 59999 - 6.00 2.32 0.38 16.48<br />
6406 RB11475 57 174 conserved hypothetical protein 6209468 6209641 4.7 5927 + 6.43 1.71 0.19 10.87<br />
6430 RB11515 74 225 conserved hypothetical protein 6224420 6224644 11.7 8687 + 6.30 4.65 0.08 1.80<br />
6456 RB11569 85 258 conserved hypothetical protein 6244071 6243814 9.4 9675 - 5.93 1.75 0.31 17.54<br />
6472 RB11603 37 114 conserved hypothetical protein 6256722 6256835 9 3998 + 7.00 2.09 0.14 6.92<br />
6524 RB11680 59 180 conserved hypothetical protein 6300635 6300456 10.1 6549 - 5.78 1.53 0.35 22.98<br />
6633 RB11909 168 507 conserved hypothetical protein 6410157 6410663 4.1 18314 + 6.09 1.91 0.30 15.80<br />
6661 RB11952 131 396 secreted protein 6438438 6438043 9.5 14509 - 5.92 2.36 0.58 24.57<br />
6719 RB12067 107 324 conserved hypothetical protein 6510236 6510559 11.9 12169 + 6.66 1.78 0.38 21.64<br />
6783 RB12196 49 150 conserved hypothetical protein 6577078 6576929 7.4 5280 - 6.33 1.65 0.25 15.05<br />
6813 RB12240 50 153 conserved hypothetical protein 6594646 6594798 10.1 5841 + 6.20 1.71 0.18 10.34<br />
6897 RB12387 50 153 conserved hypothetical protein 6665894 6665742 10.1 5841 - 5.92 2.63 0.37 13.91<br />
6944 RB12458 85 258 conserved hypothetical protein 6704719 6704976 10.9 8948 + 6.21 1.95 0.25 12.93<br />
7090 RB12732 422 1269 secreted protein containing DUF1501 6862068 6860800 6.3 45705 - 6.51 1.61 0.17 10.48<br />
7132 RB12806 205 618 conserved hypothetical protein 6911165 6910548 10.5 21914 - 6.15 2.06 0.15 7.09<br />
7133 RB12808 369 1110 secreted protein 6912350 6911241 4.6 40963 - 6.03 2.29 0.20 8.62<br />
7149 RB12830 102 309 conserved hypothetical protein 6920508 6920200 10.9 11753 - 6.60 1.83 0.25 13.55<br />
7160 RB12850 309 930 Rhodopirellula transposase 6926553 6925624 5.8 34461 - 6.34 1.71 0.17 9.83<br />
7161 RB12851 109 330 conserved hypothetical protein 6926556 6926885 7.5 12033 + 6.05 1.81 0.32 17.40<br />
7202 RB12936 580 1743 conserved hypothetical protein 6965872 6964130 5.3 66248 - 6.49 2.10 0.48 22.94<br />
7334 RB13190 50 153 conserved hypothetical protein 7083077 7083229 10.1 5841 + 6.16 1.98 0.18 9.26<br />
7351 RB13222 208 627 SOUL heme-binding protein 7094714 7094088 8.7 22867 - 6.02 5.02 0.53 10.64<br />
300min<br />
ID Locus AA Nuc Product Gene EC Start Stop IEP MW(kDa) Strand i r stdev %<br />
1961 RB3596 144 435 1868895 1869329 4.3 15958 + 5.67 2.18 0.08 3.57<br />
3221 RB5717 497 1494 soluble pyridine nucleotide transhydrogenase sthA 1.6.1.1 2985257 2983764 6 54129 - 5.30 1.73 0.19 11.15<br />
4296 RB7619 278 837 conserved hypothetical protein 4097010 4096174 5.6 30607 - 5.89 2.44 0.16 6.66<br />
6831 RB12276 110 333 conserved hypothetical protein 6605112 6605444 4.7 11911 + 6.31 3.70 0.72 19.41<br />
4149 RB7375 412 1239 Peptidase T pepT 3.4.11.4 3948831 3947593 4.4 44404 - 5.81 1.67 0.24 14.61<br />
6638 RB11919 671 2016 Dihydroxy-acid and 6-phosphogluconate dehydratase 6417713 6415698 5.3 70685 - 5.44 2.51 0.25 9.80<br />
5972 RB10733 389 1170 Glutathione-dependent formaldehyde dehydrogenase fdh 1.2.1.1 5832679 5833848 4.8 42303 + 5.90 2.14 0.19 9.04<br />
2891 RB5134 208 627 Ham1-like protein 2639251 2639877 5.7 22783 + 5.32 1.99 0.30 15.34<br />
7110 RB12762 1040 3123 Ribonucleoside-diphosphate reductase 6877208 6880330 4.9 113462 + 5.36 1.63 0.27 16.55<br />
2930 RB5200 942 2829 alpha amylase 2681425 2684253 4.6 105281 + 5.61 1.58 0.26 16.25<br />
5648 RB10127 468 1407 secreted glucose dehydrogenase 5466726 5465320 4.8 51430 - 5.94 1.98 0.25 12.76<br />
5803 RB10416 1564 4695 xylanase-like protein 3.2.1.8 5634693 5639387 4 171194 + 5.75 1.62 0.10 5.89<br />
5878 RB10566 483 1452 Gluconate transporter 5734146 5732695 9.2 50312 - 5.72 1.90 0.33 17.30<br />
2514 RB4517 117 354 Transcriptional coactivator/pterin dehydratase 2315930 2316283 5.8 13059 + 5.64 1.86 0.23 12.50<br />
2726 RB4867 210 633 SmpB RNA-binding protein SmpB 2496354 2495722 10.8 23762 - 5.89 1.87 0.29 15.35<br />
2813 RB4995 155 468 6,7-dimethyl-8-ribityllumazine synthase ribH 2.5.1.9 2569838 2569371 5.4 16629 - 5.35 2.19 0.04 1.72<br />
2892 RB5135 137 414 6-pyruvoyl tetrahydrobiopterin synthase. 4.2.3.12 2640266 2639853 5.5 15985 - 5.70 2.15 0.33 15.45<br />
5761 RB10335 244 735 Di-trans-poly-cis-decaprenylcistransferase 5590382 5589648 5 28049 - 4.97 1.91 0.47 24.42<br />
1034 RB1977 385 1158 mRNA 3'-end processing factor 1039488 1040645 6 42759 + 5.94 1.56 0.15 9.39<br />
456 RB838 253 762 transcriptional regulator, TetR family 441593 440832 5.7 28095 - 5.57 1.75 0.29 16.62<br />
2814 RB4997 514 1545 transcription termination factor Rho rho 2571441 2569897 5.9 57846 - 6.03 1.87 0.12 6.33<br />
3489 RB6201 1181 3546 TRANSCRIPTION-REPAIR COUPLING FACTOR mfd 3248886 3252431 6 130125 + 5.50 1.79 0.33 18.17<br />
4744 RB8445 141 426 Penicillinase repressor 4514475 4514050 9.9 15885 - 5.49 2.44 0.39 15.95<br />
5571 RB9999 281 846 conserved hypothetical protein 5386828 5385983 4.6 29948 - 5.48 2.49 0.19 7.67<br />
5606 RB10049 217 654 RNA polymerase ECF-type sigma factor 5419507 5420160 10.1 25205 + 6.04 3.18 0.35 11.17<br />
6160 RB11075 167 504 Bacterial regulatory protein, MarR 6005727 6005224 6.4 19491 - 5.81 1.85 0.07 3.55<br />
6090 RB10940 183 552 acetyltransferase 5934856 5935407 6.1 20469 + 5.77 2.04 0.16 7.94<br />
4187 RB7437 239 720 LexA repressor lexA 3.4.21.88 3987953 3988672 9.8 25951 + 5.45 1.65 0.03 2.07<br />
98 RB170 96 291 Transposase IS3/IS911 89333 89623 10.1 11124 + 5.51 2.59 0.10 3.76<br />
382 RB704 96 291 Transposase IS3/IS911 378137 378427 10.1 11124 + 5.68 2.26 0.22 9.65<br />
628 RB1189 96 291 Transposase IS3/IS911 606838 607128 10.1 11124 + 5.58 2.24 0.13 5.88<br />
842 RB1571 564 1695 DNA ligase:ATP-dependent DNA ligase lig 6.5.1.1 803159 804853 5.4 63326 + 5.72 2.20 0.11 5.17<br />
2381 RB4299 96 291 Transposase IS3/IS911 2214533 2214823 9.9 11182 + 5.57 2.72 0.13 4.91<br />
2463 RB4433 162 489 Ferritin and Dps 2277208 2277696 4.3 17757 + 6.43 2.39 0.39 16.15<br />
2695 RB4811 438 1317 ISPg4, transposase 2465768 2467084 9.3 48850 + 5.18 3.40 0.23 6.69<br />
3320 RB5888 96 291 Transposase IS3/IS911 3087636 3087346 10.1 11124 - 5.74 2.46 0.31 12.51<br />
3420 RB6070 201 606 HNH endonuclease family protein 3186811 3187416 9.9 23317 + 5.32 1.59 0.08 5.34<br />
3787 RB6709 96 291 Transposase IS3/IS911 3564146 3563856 10.1 11124 - 5.61 2.08 0.11 5.44
4555 RB8076 173 522 Holliday junction resolvase YqgF 3.1.-.- 4323454 4323975 5.1 18918 + 5.70 1.96 0.18 9.11<br />
4721 RB8397 708 2127 UvrABC system protein B uvrB 4489596 4487470 6.1 80350 - 5.69 2.01 0.33 16.30<br />
6481 RB11618 552 1659 type I restriction-modification system, M subunit, 6263708 6265366 6.7 62726 + 5.11 2.42 0.03 1.34<br />
6558 RB11749 96 291 Transposase IS3/IS911 6328064 6328354 10.1 11124 + 5.66 2.61 0.18 6.92<br />
6577 RB11802 96 291 Transposase IS3/IS911 6361950 6362240 10.1 11124 + 5.37 2.02 0.27 13.43<br />
7098 RB12746 294 885 Formamidopyrimidine-DNA glycolase mutM 3.2.2.23 6869843 6870727 10.3 32447 + 5.66 3.87 0.26 6.81<br />
7203 RB12940 96 291 Transposase IS3/IS911 6966246 6966536 10.1 11124 + 5.45 2.50 0.26 10.24<br />
944 RB1784 477 1434 UDP-glucose/GDP-mannose dehydrogenase 924070 925503 4.7 52259 + 5.57 1.72 0.08 4.72<br />
3620 RB6428 164 495 conserved hypothetical protein, secreted 3382816 3382322 6.5 17292 - 7.40 2.37 0.06 2.59<br />
6653 RB11941 427 1284 Glycosyl transferase, family 2 6429975 6428692 7.8 47063 - 5.65 2.30 0.32 13.68<br />
7150 RB12831 264 795 Glycosyltransferase 6921326 6920532 4.6 29501 - 6.14 2.55 0.56 21.74<br />
6425 RB11505 199 600 conserved hypothetical protein, secreted 6221829 6221230 7.5 21660 - 6.31 1.99 0.36 18.07<br />
6532 RB11690 1238 3717 preprotein translocase SecA subunit secA 6306704 6302988 4.9 139847 - 5.36 1.84 0.23 12.33<br />
731 RB1359 629 1890 Protease do precursor degP 3.4.21.- 693488 695377 6.1 66729 + 6.41 3.06 0.11 3.74<br />
1197 RB2268 282 849 peptide methionine sulfoxide reductase msrB 1.8.4.6 1191389 1190541 9.9 31115 - 6.32 1.59 0.25 15.78<br />
3138 RB5566 728 2187 cell division protein FtsH ftsH 3.4.24.- 2897569 2895383 5 79839 - 5.68 1.99 0.34 17.09<br />
3812 RB6751 859 2580 chaperone ClpB clpB 3586111 3588690 5.2 95764 + 6.51 2.12 0.45 21.35<br />
4636 RB8238 198 597 alkyl hydroperoxide reducatase 4403997 4404593 4.5 22069 + 5.35 1.87 0.11 6.11<br />
4837 RB8604 1227 3684 Cytochrome c assembly protein 4590203 4593886 5.2 134894 + 5.79 1.97 0.18 8.94<br />
5026 RB8966 660 1983 Chaperonin Cpn60/TCP-1 4777493 4779475 5.3 69819 + 6.77 2.08 0.04 2.12<br />
5028 RB8970 540 1623 GroEL 4779905 4781527 4.8 57516 + 6.76 2.31 0.47 20.33<br />
5030 RB8974 200 603 GrpE protein grpE 4782869 4783471 4.2 21927 + 6.55 2.49 0.23 9.39<br />
5092 RB9103 899 2700 ATP-dependent Clp protease, ATP-binding subunit ClpB 4866549 4863850 4.8 99439 - 6.44 2.64 0.28 10.57<br />
5094 RB9105 704 2115 Heat shock protein Hsp70 dnaK 4868834 4866720 4.6 76038 - 6.84 3.28 0.09 2.79<br />
5713 RB10242 330 993 Curved DNA-binding protein cbpA 5536118 5535126 6.7 35177 - 6.16 2.29 0.39 16.93<br />
5733 RB10279 151 456 heat shock protein, Hsp20 family 5557118 5557573 4.9 17072 + 7.33 4.02 0.42 10.40<br />
5734 RB10283 107 324 small heat shock protein 5557737 5558060 4.7 12267 + 7.46 4.53 0.12 2.59<br />
5911 RB10627 93 282 Chaperonin Cpn10 5769148 5769429 3.9 9966 + 7.24 1.70 0.21 12.18<br />
6601 RB11847 243 732 Peptide methionine sulfoxide reductase msrA msrA 1.8.4.6 6379337 6380068 6.5 26839 + 6.15 1.90 0.19 9.81<br />
6619 RB11878 168 507 peptide methionine sulfoxide reductase msrA msrA3 1.8.4.6 6396598 6397104 4.8 19100 + 5.86 2.06 0.45 21.62<br />
6727 RB12080 492 1479 Protein-L-isoaspartate(D-aspartate) O-methyltransferase 2.1.1.77 6517231 6515753 8.9 53385 - 5.77 1.71 0.14 7.92<br />
5783 RB10378 144 435 Thioredoxin trxA 5610497 5610063 4.6 15274 - 5.81 1.70 0.29 16.96<br />
791 RB1477 538 1617 arylsulfatase precursor ARSA 3.1.6.8 755327 756943 5.5 59805 + 5.26 1.59 0.14 8.78<br />
5301 RB9498 518 1557 Arylsulfatase A Arsa 3.1.6.8 5099139 5100695 6 58375 + 5.27 2.04 0.29 14.12<br />
5967 RB10727 276 831 manganese-containing catalase 5830775 5831605 5 30055 + 6.91 2.59 0.07 2.77<br />
708 RB1323 429 1290 Protein of unknown function DUF894, DitE 672945 671656 11.8 45426 - 5.98 1.67 0.20 11.94<br />
592 RB1122 449 1350 secretion protein lktD 578202 576853 5.3 50153 - 5.50 1.54 0.20 12.81<br />
1759 RB3253 357 1074 ABC transporter, ATP-binding protein yhcG 1675049 1673976 5.7 39316 - 5.81 1.59 0.20 12.83<br />
3648 RB6469 252 759 ABC transporter ATP-binding protein 3419515 3420273 5.6 27684 + 5.81 2.60 0.19 7.18<br />
4039 RB7166 312 939 ABC-type multidrug transport system, ATPase component 3800705 3801643 5.4 35063 + 5.47 1.78 0.14 8.12<br />
5260 RB9420 663 1992 Lipid A export ATP-binding/permease protein msbA 5055368 5053377 9.3 73442 - 5.96 1.98 0.12 6.28<br />
5727 RB10270 537 1614 phytoene dehydrogenase carC 1.14.99.- 5551969 5550356 5.5 60117 - 5.72 2.82 0.23 8.17<br />
6112 RB10981 600 1803 MULTIDRUG EFFLUX SYSTEM PROTEIN 5948851 5950653 4.5 66407 + 6.01 1.60 0.21 13.32<br />
6113 RB10984 1115 3348 Acriflavin resistance protein 5950650 5953997 4.6 122096 + 5.81 1.62 0.12 7.15<br />
6655 RB11943 534 1605 phytoene desaturase 6432376 6430772 5.8 57855 - 5.75 1.56 0.28 17.69<br />
3933 RB6973 278 837 tellurite resistance protein 3710584 3711420 5 30569 + 5.48 2.67 0.45 16.79<br />
3950 RB7004 271 816 Short-chain dehydrogenase/reductase SDR 3727471 3726656 9.7 29211 - 5.80 1.68 0.16 9.80<br />
6414 RB11488 265 798 oxidoreductase, short-chain dehydrogenase/reductase family 6213893 6214690 5.1 27687 + 6.07 2.04 0.15 7.12<br />
158 RB282 342 1029 conserved hypothetical protein, membrane 141075 140047 7.4 37446 - 5.97 1.93 0.08 4.07<br />
228 RB392 218 657 hydrolase 204269 204925 4.3 24145 + 5.82 1.96 0.04 1.99<br />
745 RB1391 538 1617 cell division inhibitor- 710780 709164 7.8 59400 - 6.36 2.02 0.26 12.71<br />
846 RB1577 860 2583 ATP dependent DNA helicase 806289 808871 9.9 95417 + 5.93 1.65 0.19 11.59<br />
952 RB1798 364 1095 methanol dehydrogenase regulatory protein 930673 931767 4.6 40611 + 5.50 2.12 0.20 9.63<br />
1608 RB2968 386 1161 GTPase EngC engC 3.6.1.- 1529085 1530245 8.6 41916 + 5.88 2.14 0.22 10.20<br />
2547 RB4579 194 585 Protein of unknown function, UPF0054 2346073 2345489 3.7 20879 - 5.50 2.07 0.39 18.61<br />
2548 RB4580 794 2385 metal-dependent phosphohydrolase 2348454 2346070 8.7 86766 - 5.91 1.50 0.31 20.83<br />
2571 RB4619 481 1446 dehydrogenase 2363086 2364531 5.1 52511 + 6.21 1.64 0.29 17.81<br />
2896 RB5140 613 1842 hypothetical protein 2641937 2643778 5.5 67565 + 5.49 1.99 0.32 15.87<br />
3602 RB6395 778 2337 conserved hypothetical protein 3364879 3367215 4.6 85714 + 6.02 2.44 0.04 1.65<br />
3936 RB6982 286 861 ABC transporter ATP-binding protein 3715019 3715879 5 31150 + 5.54 2.80 0.12 4.43<br />
4037 RB7161 106 321 protein containing pyrophosphohydrolase domain 3798000 3798320 4.3 11777 + 5.35 1.72 0.43 24.86<br />
4603 RB8173 339 1020 MoxR protein 4366881 4367900 5.1 37556 + 5.91 2.05 0.07 3.31<br />
4622 RB8214 589 1770 membrane protein 4392948 4391179 4.5 63301 - 6.10 1.76 0.15 8.45<br />
4915 RB8768 347 1044 methanol dehydrogenase regulatory protein (MoxR protein) 4661498 4660455 4.8 38221 - 5.38 1.59 0.26 16.62<br />
6114 RB10985 207 624 HD domain protein 5954633 5954010 5.7 23118 - 5.13 2.61 0.29 11.00<br />
6267 RB11262 331 996 diacylglycerol kinase 6108302 6107307 5 35708 - 5.90 1.93 0.14 7.24<br />
6370 RB11438 54 165 conserved hypothetical protein 6197926 6197762 11.3 6209 - 5.39 2.12 0.18 8.39<br />
6641 RB11923 231 696 Electron transport protein SCO1/SenC 6419736 6419041 4.3 25792 - 5.80 1.77 0.22 12.18<br />
7201 RB12934 253 762 conserved hypothetical protein 6964029 6963268 4.8 27657 - 5.51 1.55 0.27 17.58<br />
7369 RB13257 286 861 2-hydroxy-6-ketonona-2,4-dienedioic acid hydrolase bphD 3.7.1.- 7105590 7106450 4.6 32540 + 5.89 2.67 0.26 9.67<br />
1502 RB2774 82 249 conserved hypothetical protein 1443786 1444034 4.4 9450 + 6.21 1.89 0.27 14.44<br />
2438 RB4395 170 513 carboxymuconolactone decarboxylase 4.1.1.44 2262023 2262535 4.7 18791 + 6.05 1.70 0.28 16.41<br />
2453 RB4419 178 537 Ferritin and Dps 2272912 2273448 6.2 18995 + 6.20 2.23 0.37 16.37<br />
3454 RB6139 187 564 Protein of unknown function, UPF0157 3226050 3226613 5.7 21081 + 5.29 2.02 0.26 12.72<br />
4294 RB7616 221 666 protein containing DUF162 4094752 4094087 4.7 23976 - 5.73 2.44 0.16 6.55<br />
4604 RB8174 369 1110 conserved hypothetical protein 4367851 4368960 9.9 40726 + 6.05 1.50 0.18 11.94<br />
4911 RB8759 747 2244 conserved hypothetical protein, membrane 4657568 4655325 4.4 82203 - 5.73 1.59 0.36 22.67<br />
6131 RB11013 218 657 conserved hypothetical protein, membrane 5971802 5971146 4.9 24214 - 5.28 1.56 0.25 16.03<br />
6217 RB11176 153 462 protein containing DUF442 6065605 6065144 4.8 17161 - 6.36 1.68 0.29 17.39<br />
6348 RB11399 132 399 Putative thiol-disulphide oxidoreductase DCC 6179167 6179565 6.5 15047 + 6.96 2.58 0.16 6.22<br />
80 RB140 987 2964 serine/threonine protein kinase pkn3 2.7.1.37 61578 64541 5.2 106565 + 5.82 1.90 0.08 4.46<br />
2510 RB4511 1637 4914 two-component sensor histidine kinase 2307911 2312824 4.8 183570 + 5.44 1.64 0.29 17.68<br />
3294 RB5848 140 423 dnaK suppressor protein, 3066328 3065906 4.6 15633 - 6.35 2.39 0.18 7.46<br />
3615 RB6417 249 750 conserved hypothetical protein 3378045 3378794 5.9 27679 + 6.55 1.84 0.26 14.24<br />
5758 RB10330 860 2583 sensory box sensor histidine kinase/response regulator 5587514 5584932 4.9 94952 - 5.69 2.87 0.22 7.63<br />
6266 RB11260 121 366 dnaK suppressor protein, 6107202 6106837 5.2 13639 - 6.02 1.85 0.23 12.65<br />
79 RB138 214 645 Sigma factor, ECF-like 60872 61516 4.8 24057 + 5.71 3.59 0.24 6.55<br />
234 RB402 151 456 hypothetical protein 212820 213275 4.4 17306 + 5.21 2.14 0.35 16.43<br />
300 RB520 375 1128 conserved hypothetical protein 284989 286116 4.8 41300 + 5.86 2.31 0.41 17.82<br />
301 RB521 63 192 hypothetical protein 286399 286208 10.7 7040 - 6.10 3.40 0.26 7.64<br />
319 RB550 137 414 secreted protein 306709 306296 12.2 15112 - 5.83 2.04 0.08 3.90<br />
362 RB663 79 240 hypothetical protein 357608 357369 11.4 8792 - 6.43 2.32 0.26 11.18<br />
366 RB671 122 369 hypothetical protein 359218 359586 10.7 13383 + 6.09 2.03 0.43 21.10<br />
392 RB723 60 183 hypothetical protein 388051 388233 7.3 6624 + 5.71 1.53 0.21 13.60<br />
507 RB934 375 1128 Putative transposase 494203 493076 9.9 41881 - 5.26 1.63 0.18 11.31<br />
517 RB952 258 777 hypothetical protein 502128 502904 10.3 28841 + 5.39 1.79 0.31 17.11<br />
662 RB1239 155 468 hypothetical protein 632500 632033 9.7 17106 - 5.63 1.54 0.17 10.98<br />
667 RB1252 665 1998 secreted protein 638429 640426 3.8 72471 + 5.23 2.11 0.46 21.60<br />
669 RB1255 51 156 hypothetical protein 640910 640755 12.3 5933 - 5.21 2.13 0.20 9.59<br />
670 RB1256 51 156 hypothetical protein 641044 640889 12 5840 - 4.75 1.57 0.27 17.19<br />
707 RB1322 35 108 hypothetical protein 671526 671633 12.8 3914 + 5.95 2.46 0.45 18.31<br />
709 RB1324 77 234 hypothetical protein 673169 672936 10.9 8459 - 5.85 1.84 0.26 13.90<br />
742 RB1386 350 1053 inosine-uridine preferring nucleoside hydrolase 708536 707484 4.8 38419 - 5.08 1.66 0.39 23.23<br />
743 RB1389 152 459 hypothetical protein 708892 708434 11 16822 - 5.90 1.69 0.18 10.62<br />
744 RB1390 149 450 membrane protein 709167 708718 9.9 16437 - 6.06 1.61 0.03 2.11<br />
747 RB1394 78 237 hypothetical protein 711994 711758 10.1 8765 - 6.13 2.54 0.30 11.99<br />
748 RB1395 319 960 secreted protein 712097 713056 5.5 35212 + 6.62 2.42 0.20 8.39<br />
804 RB1501 50 153 hypothetical protein 769419 769571 7.3 5710 + 5.58 3.20 0.17 5.19<br />
844 RB1573 36 111 hypothetical protein 805094 804984 11.9 4165 - 5.27 2.19 0.35 15.95<br />
913 RB1722 651 1956 hypothetical protein 897688 899643 5.4 70377 + 5.84 4.18 0.54 12.84<br />
915 RB1728 285 858 membrane protein containing DUF1206 899954 900811 9.9 30055 + 5.98 2.42 0.43 17.89<br />
916 RB1729 120 363 hypothetical protein 901146 900784 7.7 13145 - 5.51 2.95 0.15 5.25<br />
933 RB1760 395 1188 membrane protein 915210 914023 7.8 43386 - 5.45 1.82 0.22 11.96<br />
936 RB1767 907 2724 60-kDa cysteine-rich outer membrane protein precursor omcB 919059 916336 4.2 96071 - 5.36 1.80 0.24 13.53<br />
947 RB1789 243 732 conserved hypothetical protein 925861 926592 9.4 27028 + 5.96 1.85 0.43 23.23<br />
1371 RB2525 76 231 hypothetical protein 1320284 1320054 6.1 8326 - 5.84 1.56 0.28 18.03<br />
1477 RB2724 266 801 membrane protein 1419817 1419017 7.4 29523 - 6.30 1.64 0.19 11.77<br />
1515 RB2804 201 606 hypothetical protein 1458645 1458040 5.3 22766 - 5.82 2.36 0.12 5.10<br />
1518 RB2809 226 681 hypothetical protein 1459745 1459065 9.1 25318 - 6.19 1.77 0.04 2.46<br />
1548 RB2864 596 1791 hypothetical protein 1490057 1491847 3.9 62722 + 4.87 2.02 0.02 0.97<br />
1549 RB2867 697 2094 hypothetical protein 1491909 1494002 4.7 78102 + 5.41 1.50 0.20 13.18<br />
1606 RB2963 119 360 hypothetical protein 1526707 1527066 12.2 13440 + 6.41 1.89 0.08 4.17<br />
1728 RB3196 109 330 hypothetical protein 1650093 1650422 10.1 12287 + 6.88 2.20 0.10 4.67
1744 RB3228 962 2889 protein containing DUF1585 1656635 1659523 4.6 107564 + 5.43 2.87 0.52 18.12<br />
1957 RB3587 113 342 hypothetical protein 1864796 1864455 11.8 13003 - 5.57 1.54 0.16 10.56<br />
2169 RB3929 54 165 hypothetical protein 2034407 2034243 10.7 5796 - 4.94 1.83 0.36 19.73<br />
2396 RB4322 457 1374 transposase and inactivated derivative 2226866 2228239 8.4 52492 + 5.32 2.02 0.13 6.51<br />
2398 RB4325 48 147 Planctomycete PGAMP 2228477 2228623 12.5 5403 + 5.16 2.30 0.24 10.46<br />
2405 RB4341 109 330 hypothetical protein 2234849 2235178 7.5 12018 + 4.91 1.98 0.09 4.70<br />
2429 RB4381 59 180 hypothetical protein 2255621 2255442 10.7 7087 - 6.92 1.79 0.08 4.32<br />
2431 RB4383 545 1638 decarboxylase 2258768 2257131 5.4 59839 - 6.53 1.57 0.32 20.35<br />
2440 RB4397 55 168 protein containing DUF1560 2263976 2264143 10.5 5816 + 6.11 2.80 0.44 15.71<br />
2446 RB4405 119 360 conserved hypothetical protein 2266894 2266535 4.3 13746 - 5.85 1.71 0.39 22.65<br />
2458 RB4426 60 183 hypothetical protein 2275786 2275604 6.3 6516 - 5.16 2.65 0.21 7.87<br />
2461 RB4429 89 270 conserved hypothetical protein 2275996 2276265 4.9 10034 + 6.86 1.77 0.19 10.84<br />
2465 RB4438 160 483 Pyridoxamine 5'-phosphate oxidase- 2278247 2278729 4.4 18043 + 6.15 2.88 0.68 23.58<br />
2475 RB4457 98 297 hypothetical protein 2285628 2285924 10 10915 + 5.62 1.53 0.21 13.86<br />
2478 RB4460 422 1269 conserved hypothetical protein, membrane 2289696 2288428 4.8 48179 - 6.07 1.74 0.10 6.02<br />
2585 RB4643 96 291 conserved hypothetical protein 2377555 2377845 8.9 11064 + 5.57 1.91 0.15 7.85<br />
2607 RB4676 221 666 hypothetical protein 2391079 2390414 10.4 23751 - 5.64 1.65 0.18 11.21<br />
2608 RB4678 199 600 secreted protein 2390618 2391217 4.2 20927 + 6.41 2.11 0.27 12.99<br />
2609 RB4679 175 528 secreted protein 2391214 2391741 4.2 18596 + 6.52 1.84 0.39 20.98<br />
2617 RB4698 86 261 hypothetical protein 2397601 2397861 12.2 9541 + 5.38 2.83 0.20 7.20<br />
2696 RB4812 92 279 secreted protein 2467011 2467289 12.5 10014 + 5.31 2.73 0.02 0.75<br />
2850 RB5064 648 1947 secreted protein containing DUF1585 2605707 2607653 4.6 72145 + 5.43 1.64 0.27 16.39<br />
2951 RB5238 73 222 hypothetical protein 2697775 2697996 10.6 7866 + 5.53 3.64 0.27 7.49<br />
3022 RB5370 309 930 Rhodopirellula transposase 2774826 2773897 5.9 34530 - 5.69 2.23 0.22 9.70<br />
3023 RB5371 109 330 hypothetical protein 2774829 2775158 7.5 12033 + 5.11 2.23 0.28 12.78<br />
3118 RB5523 91 276 hypothetical protein 2860984 2860709 6.3 9939 - 5.71 1.98 0.32 16.22<br />
3130 RB5551 663 1992 hypothetical protein 2889101 2891092 5.7 70932 + 5.70 2.59 0.40 15.52<br />
3139 RB5567 66 201 hypothetical protein 2897766 2897566 12.1 7106 - 5.83 2.64 0.29 11.15<br />
3303 RB5866 441 1326 ISXo8 transposase 3076242 3077567 9.2 50613 + 5.36 1.52 0.19 12.73<br />
3346 RB5938 370 1113 hypothetical protein 3113484 3112372 5.6 41837 - 6.14 4.02 0.25 6.18<br />
3347 RB5939 39 120 hypothetical protein 3113462 3113581 10.9 4404 + 5.22 2.50 0.28 11.01<br />
3457 RB6144 38 117 hypothetical protein 3228305 3228189 11.1 4314 - 5.63 2.46 0.33 13.22<br />
3472 RB6168 208 627 hypothetical protein 3236689 3237315 12.2 22575 + 5.45 1.74 0.28 16.33<br />
3482 RB6188 68 207 hypothetical protein 3244384 3244178 9.6 6986 - 5.38 1.54 0.07 4.63<br />
3503 RB6233 629 1890 membrane protein 3271181 3269292 5.4 69401 - 6.84 1.67 0.05 2.85<br />
3531 RB6282 200 603 conserved hypothetical protein 3302795 3303397 4.8 22451 + 5.37 1.93 0.09 4.67<br />
3596 RB6388 144 435 hypothetical protein 3360906 3360472 9.1 16558 - 5.60 2.43 0.19 8.01<br />
3621 RB6429 61 186 hypothetical protein 3382998 3382813 12.1 7095 - 7.35 1.79 0.15 8.36<br />
3771 RB6685 72 219 hypothetical protein 3554265 3554047 12.2 8077 - 5.54 1.85 0.07 3.80<br />
3784 RB6706 37 114 hypothetical protein 3560942 3560829 7.3 4375 - 5.45 1.96 0.22 11.09<br />
3888 RB6888 420 1263 secreted protein containing DUF1573 3662449 3661187 4.3 45773 - 5.64 3.35 0.27 7.97<br />
3910 RB6928 160 483 hypothetical protein 3678479 3678961 4.1 16965 + 5.87 1.80 0.43 24.13<br />
3969 RB7039 202 609 hypothetical protein 3743573 3742965 7.9 23105 - 5.41 1.85 0.22 11.71<br />
4021 RB7128 85 258 hypothetical protein 3782627 3782370 4.8 9275 - 5.44 1.52 0.13 8.48<br />
4151 RB7381 243 732 membrane protein 3950326 3949595 7.4 26253 - 5.78 3.08 0.42 13.72<br />
4157 RB7389 375 1128 Putative transposase 3953590 3954717 9.9 41895 + 5.42 1.82 0.06 3.25<br />
4583 RB8132 177 534 hypothetical protein 4349972 4350505 10.8 19786 + 6.21 1.86 0.14 7.39<br />
4595 RB8153 486 1461 conserved hypothetical protein 4359392 4357932 4.8 54199 - 5.45 2.53 0.14 5.48<br />
4681 RB8325 296 891 hypothetical protein 4447029 4446139 6.8 32118 - 5.39 2.14 0.19 8.84<br />
4704 RB8365 90 273 membrane protein 4463903 4463631 11.7 9405 - 6.60 1.58 0.27 16.93<br />
4727 RB8409 97 294 hypothetical protein 4495547 4495840 8.9 10905 + 5.57 1.50 0.06 4.21<br />
4791 RB8527 330 993 protein containing DUF1559 4549097 4550089 6.6 35645 + 5.70 1.54 0.12 8.03<br />
4836 RB8601 417 1254 hypothetical protein 4588912 4590165 4.2 45382 + 5.86 1.78 0.29 16.46<br />
5023 RB8959 662 1989 hypothetical protein 4777121 4775133 5.3 70536 - 6.46 1.58 0.39 24.36<br />
5027 RB8969 101 306 Chaperonin Cpn10 4779554 4779859 4.9 10628 + 6.67 2.80 0.43 15.29<br />
5031 RB8975 110 333 hypothetical protein 4783888 4783556 10.4 12068 - 6.28 2.44 0.30 12.30<br />
5038 RB8987 48 147 hypothetical protein 4790856 4790710 9.1 5309 - 5.10 2.86 0.10 3.46<br />
5093 RB9104 45 138 hypothetical protein 4866696 4866559 9 4979 - 6.56 1.79 0.24 13.61<br />
5157 RB9230 107 324 hypothetical protein 4936673 4936996 9.9 11900 + 5.98 2.10 0.45 21.36<br />
5158 RB9231 109 330 hypothetical protein 4937008 4936679 7.5 12033 - 4.93 2.14 0.39 18.40<br />
5159 RB9232 309 930 Rhodopirellula transposase 4937011 4937940 5.9 34530 + 5.32 1.69 0.39 23.06<br />
5346 RB9577 51 156 hypothetical protein 5150501 5150346 9.3 5646 - 5.31 1.57 0.16 10.08<br />
5456 RB9787 61 186 hypothetical protein 5270904 5270719 6.1 6701 - 5.29 2.73 0.24 8.93<br />
5473 RB9811 102 309 secreted protein 5284365 5284673 11.7 11169 + 5.65 2.44 0.23 9.35<br />
5524 RB9907 433 1302 ISXo8 transposase 5338185 5339486 9.4 49646 + 5.45 1.83 0.09 5.12<br />
5550 RB9955 452 1359 secreted protein containing DUF1552 5363605 5362247 5.6 50315 - 5.95 2.42 0.23 9.47<br />
5552 RB9959 65 198 hypothetical protein 5366417 5366220 11.9 7376 - 6.11 2.01 0.12 6.18<br />
5576 RB10007 313 942 conserved hypothetical protein 5390130 5391071 5 34128 + 6.69 2.43 0.11 4.70<br />
5607 RB10051 1210 3633 conserved hypothetical protein 5420157 5423789 4.6 135400 + 5.83 1.92 0.16 8.48<br />
5654 RB10137 73 222 protein containing DUF1590 5469772 5469551 12.1 8049 - 5.46 2.03 0.15 7.22<br />
5738 RB10292 581 1746 conserved hypothetical protein, secreted 5559646 5561391 8.3 66858 + 5.91 2.65 0.23 8.73<br />
5741 RB10299 455 1368 ATP-dependent carboligase related to biotin carboxylase (ORFY) c1 5563036 5564403 10 49949 + 7.02 2.65 0.07 2.65<br />
5771 RB10350 163 492 Thioredoxin 5596878 5596387 9.7 18371 - 6.04 2.03 0.44 21.64<br />
5806 RB10423 2079 6240 hemolysin 5640680 5646919 3.6 219600 + 5.56 2.28 0.11 4.85<br />
5815 RB10440 49 150 hypothetical protein 5655693 5655842 11.5 5080 + 6.84 1.68 0.31 18.48<br />
5874 RB10556 134 405 conserved hypothetical protein, membrane 5727064 5727468 10.5 15094 + 5.23 2.12 0.30 14.06<br />
5876 RB10560 934 2805 hypothetical protein 5731867 5729063 4.6 100880 - 5.72 2.23 0.22 9.86<br />
5910 RB10623 60 183 secreted protein 5769140 5768958 3.9 6284 - 7.86 1.74 0.14 8.15<br />
5912 RB10629 539 1620 GroEL 5769500 5771119 4.9 57511 + 7.17 2.19 0.25 11.43<br />
5931 RB10665 200 603 hypothetical protein 5789912 5790514 5.3 21401 + 6.32 1.67 0.05 2.77<br />
5968 RB10728 132 399 secreted protein 5831649 5832047 9.9 13833 + 6.38 3.22 0.30 9.26<br />
6053 RB10877 453 1362 membrane protein 5905128 5903767 4.3 48882 - 5.38 2.78 0.30 10.80<br />
6067 RB10896 161 486 secreted protein 5917040 5916555 10 17034 - 5.85 2.06 0.16 7.99<br />
6087 RB10936 56 171 hypothetical protein 5934348 5934518 12.5 6620 + 5.59 2.91 0.28 9.46<br />
6094 RB10948 153 462 conserved hypothetical protein, secreted 5938575 5939036 4.2 17070 + 8.45 1.55 0.15 9.89<br />
6095 RB10950 233 702 membrane or secreted protein 5939124 5939825 4.9 25130 + 5.72 1.55 0.15 9.87<br />
6108 RB10974 234 705 hypothetical protein 5946559 5947263 5.3 25956 + 5.50 1.50 0.16 10.64<br />
6124 RB11000 224 675 conserved hypothetical protein, membrane 5964054 5963380 8.8 24185 - 5.95 1.80 0.32 17.89<br />
6177 RB11109 109 330 hypothetical protein 6022597 6022268 7.5 12033 - 5.34 1.58 0.35 22.46<br />
6178 RB11110 309 930 Rhodopirellula transposase 6022600 6023529 5.8 34570 + 5.76 1.76 0.20 11.39<br />
6347 RB11397 207 624 SOUL heme-binding protein 6178540 6179163 8.7 23072 + 6.86 2.66 0.12 4.51<br />
6424 RB11504 72 219 conserved hypothetical protein 6221285 6221067 10.7 8212 - 6.02 3.56 0.41 11.66<br />
6430 RB11515 74 225 conserved hypothetical protein 6224420 6224644 11.7 8687 + 5.45 3.72 0.40 10.73<br />
6455 RB11566 195 588 conserved hypothetical protein 6243111 6243698 10.8 22135 + 5.97 2.51 0.27 10.95<br />
6472 RB11603 37 114 conserved hypothetical protein 6256722 6256835 9 3998 + 6.32 2.41 0.15 6.28<br />
6475 RB11612 81 246 conserved hypothetical protein 6260715 6260470 12 9246 - 5.42 1.98 0.21 10.83<br />
6511 RB11667 174 525 conserved hypothetical protein 6293231 6292707 10 19482 - 5.66 2.24 0.08 3.45<br />
6556 RB11746 503 1512 protein containing DUF1501 6326162 6327673 5.8 55474 + 5.66 2.00 0.18 8.98<br />
6606 RB11855 101 306 conserved hypothetical protein 6383765 6383460 12.5 11384 - 5.20 1.59 0.10 6.47<br />
6633 RB11909 168 507 conserved hypothetical protein 6410157 6410663 4.1 18314 + 5.22 2.35 0.32 13.55<br />
6673 RB11977 196 591 conserved hypothetical protein 6455958 6456548 9.5 21922 + 5.55 2.45 0.37 15.23<br />
6691 RB12009 514 1545 conserved hypothetical protein 6479495 6477951 4.8 54935 - 5.39 1.92 0.09 4.91<br />
6719 RB12067 107 324 conserved hypothetical protein 6510236 6510559 11.9 12169 + 5.96 2.08 0.37 17.94<br />
6729 RB12082 244 735 haloacid dehalogenase-type hydrolase 6519608 6518874 4.3 26835 - 5.49 2.38 0.30 12.50<br />
6760 RB12150 53 162 conserved hypothetical protein 6556901 6557062 4.1 5983 + 6.37 2.50 0.42 16.91<br />
6783 RB12196 49 150 conserved hypothetical protein 6577078 6576929 7.4 5280 - 5.56 1.97 0.10 4.82<br />
6812 RB12239 433 1302 ISXo8 transposase 6593334 6594635 9.4 49646 + 5.33 1.65 0.11 6.54<br />
6815 RB12243 55 168 conserved hypothetical protein 6596256 6596423 10.5 5848 + 5.81 3.19 0.47 14.73<br />
6817 RB12247 74 225 conserved hypothetical protein 6597048 6596824 6.3 8306 - 6.10 3.47 0.81 23.26<br />
6861 RB12323 248 747 conserved hypothetical protein 6627001 6627747 4.4 27447 + 5.21 1.72 0.31 17.98<br />
6878 RB12348 260 783 FxsA cytoplasmic membrane protein 6645520 6644738 11.6 28277 - 6.31 1.72 0.40 23.40<br />
6889 RB12375 446 1341 conserved hypothetical protein 6659741 6661081 4.9 48379 + 6.30 1.72 0.07 3.78<br />
6950 RB12471 85 258 conserved hypothetical protein 6712442 6712185 9.7 9841 - 5.62 1.53 0.11 6.94<br />
7088 RB12727 680 2043 conserved hypothetical protein 6860301 6858259 5.7 73171 - 5.94 2.45 0.52 21.08<br />
7090 RB12732 422 1269 secreted protein containing DUF1501 6862068 6860800 6.3 45705 - 5.50 3.01 0.13 4.21<br />
7092 RB12737 65 198 conserved hypothetical protein 6863917 6864114 9.1 6753 + 6.77 1.87 0.12 6.40<br />
7095 RB12741 905 2718 conserved hypothetical protein 6868329 6865612 4.2 97067 - 5.17 2.68 0.08 3.11<br />
7132 RB12806 205 618 conserved hypothetical protein 6911165 6910548 10.5 21914 - 5.10 2.30 0.12 5.35<br />
7133 RB12808 369 1110 secreted protein 6912350 6911241 4.6 40963 - 5.12 2.46 0.48 19.42<br />
7148 RB12829 159 480 conserved hypothetical protein 6919813 6920292 9.5 17677 + 5.79 2.23 0.20 8.91<br />
7149 RB12830 102 309 conserved hypothetical protein 6920508 6920200 10.9 11753 - 5.97 2.50 0.43 17.05<br />
7160 RB12850 309 930 Rhodopirellula transposase 6926553 6925624 5.8 34461 - 5.35 2.16 0.21 9.55<br />
7161 RB12851 109 330 conserved hypothetical protein 6926556 6926885 7.5 12033 + 5.21 2.36 0.23 9.73
7197 RB12925 39 120 protein containing DUF1560 6960998 6960879 9.9 4014 - 5.78 1.50 0.19 12.48<br />
7202 RB12936 580 1743 conserved hypothetical protein 6965872 6964130 5.3 66248 - 6.28 2.84 0.24 8.49<br />
7205 RB12942 500 1503 serine/threonine protein kinase related protein 6969009 6967507 5 55215 - 5.28 1.59 0.17 10.45<br />
7256 RB13053 142 429 secreted protein 7008596 7009024 5.6 14910 + 5.62 1.99 0.16 8.05<br />
7270 RB13082 73 222 conserved hypothetical protein 7020677 7020898 11.7 8266 + 6.07 1.67 0.22 13.37<br />
7271 RB13084 179 540 protein containing DUF820 7020834 7021373 4.5 20200 + 5.23 2.05 0.07 3.54<br />
7278 RB13093 158 477 secreted protein 7025577 7025101 9.6 17298 - 5.15 2.38 0.00 0.12<br />
7280 RB13095 229 690 conserved hypothetical protein 7026753 7027442 7.7 24080 + 5.48 1.81 0.20 11.11<br />
7281 RB13097 171 516 membrane protein 7027310 7026795 4.5 17420 - 4.82 1.62 0.36 21.92<br />
7293 RB13115 81 246 conserved hypothetical protein 7036614 7036369 9.8 8735 - 5.92 1.55 0.15 9.81<br />
7295 RB13117 156 471 nitrogen regulation protein 7036595 7037065 4.6 17819 + 5.83 1.64 0.19 11.75<br />
7309 RB13142 125 378 Planctomycete PGAMP 7051226 7050849 12.5 14087 - 5.01 2.95 0.09 3.00<br />
7350 RB13221 322 969 secreted protein 7093018 7093986 4.5 35324 + 5.92 1.96 0.12 6.23
Heat repressed<br />
10min ID Locus AA Nuc Product Gene EC Start Stop IEP MW(kDa) Strand Intensity Ratio Stdev %<br />
238 RB410 342 1029 anthranilate phosphoribosyltransferase trpD 2.4.2.18 219986 218958 5.1 35653 - 6.19 -1.13 0.10 8.47<br />
1967 RB3603 344 1035 secreted protein 1875461 1874427 4.6 37999 - 6.12 -1.59 0.22 13.96<br />
2359 RB4269 282 849 glutamic acid specific endopeptidase, GSE=serine endopeptidase 2185049 2184201 5.6 31283 - 6.14 -1.55 0.18 11.38<br />
6865 RB12327 686 2061 TGF-beta receptor, type I/II extracellular region 6633918 6631858 4.5 75645 - 6.02 -1.29 0.04 3.46<br />
2192 RB3967 544 1635 sodium:proline/pantothenate symporter (proline/pantothenate permease) 2053237 2054871 9.4 59256 + 6.13 -1.17 0.12 9.93<br />
1423 RB2627 342 1029 Glyceraldehyde 3-phosphate dehydrogenase gap 1.2.1.12 1364551 1365579 5.4 36716 + 6.92 -1.44 0.07 4.53<br />
179 RB314 309 930 malonyl CoA-acyl carrier protein transacylase fabD 2.3.1.39 156968 157897 4.4 32349 + 7.17 -1.91 0.12 6.29<br />
181 RB318 81 246 Acyl carrier protein acpP 158928 159173 3.7 9018 + 6.63 -1.25 0.09 6.78<br />
2733 RB4876 397 1194 delta 9 acyl-lipid fatty acid desaturase 1.14.19.- 2506214 2505021 9.5 44741 - 7.98 -1.19 0.09 7.29<br />
145 RB264 64 195 Ribosomal protein S21 132677 132871 12.3 8074 + 6.59 -1.03 0.17 16.90<br />
2286 RB4143 499 1500 Amidase 2130113 2131612 4.5 52330 + 6.09 -1.02 0.13 12.54<br />
4014 RB7115 118 357 Ribosomal protein L20 3779618 3779262 12.2 13584 - 6.49 -1.14 0.09 7.83<br />
4427 RB7836 107 324 Ribosomal L23 protein 4194060 4194383 10.8 12651 + 7.68 -1.02 0.05 5.10<br />
4429 RB7838 89 270 Ribosomal protein S19/S15 4195376 4195645 10.8 9943 + 6.86 -1.05 0.24 22.73<br />
4435 RB7850 122 369 Ribosomal protein L14b/L23e 4198081 4198449 11 13526 + 6.91 -1.05 0.11 10.82<br />
4440 RB7856 181 546 50S ribosomal protein L6 rplF 4200306 4200851 10 19733 + 6.71 -1.13 0.19 16.52<br />
4750 RB8459 55 168 30S ribosomal protein S18 rpsR 4518496 4518663 10.5 6203 + 6.82 -1.39 0.19 13.64<br />
4824 RB8580 113 342 sigma-54 modulation protein 4577393 4577734 5.7 12706 + 7.03 -1.03 0.06 5.66<br />
5832 RB10471 151 456 Ribosomal protein L13 5678759 5678304 10 17369 - 7.27 -1.15 0.08 6.97<br />
7154 RB12839 225 678 Ribosomal protein L1 6921934 6922611 9.8 24159 + 7.34 -1.82 0.07 4.05<br />
4459 RB7894 398 1197 translation elongation factor EF-Tu 4217484 4218680 5.2 43184 + 7.70 -1.75 0.09 4.95<br />
1919 RB3521 108 327 ArsR family transcriptional regulatory protein 1823417 1823743 9.6 11920 + 6.23 -1.02 0.16 15.49<br />
2610 RB4681 65 198 cold shock protein cspA 2391934 2392131 4.7 6982 + 7.32 -1.93 0.02 1.06<br />
5823 RB10458 206 621 Rnpc2-prov protein 5671130 5670510 10.3 21043 - 6.71 -1.47 0.12 8.29<br />
3528 RB6276 105 318 Histone-like bacterial DNA-binding protein 3297504 3297187 10.4 11299 - 7.66 -2.04 0.06 3.14<br />
1615 RB2978 152 459 nudix (nucleoside diphosphate linked moiety X)-type motif 2 1536161 1536619 5.9 17438 + 6.10 -1.00 0.12 12.15<br />
1349 RB2500 306 921 NAD-dependent epimerase/dehydratase 1307899 1306979 8.5 33992 - 6.22 -1.06 0.08 7.67<br />
2244 RB4061 488 1467 Peptidase A24A, prepilin type IV:Peptidase A24A, N-terminal 2095498 2096964 9.7 54251 + 6.05 -1.07 0.03 3.06<br />
1850 RB3403 491 1476 arylsulfatase precursor arsA 3.1.6.8 1756892 1755417 5.5 55139 - 6.01 -1.02 0.19 18.89<br />
1644 RB3032 477 1434 Major facilitator superfamily MFS_1 1567813 1569246 9.8 52109 + 6.04 -1.07 0.06 5.40<br />
1332 RB2479 273 822 conserved hypothetical protein 1296683 1295862 5.5 30436 - 6.09 -1.25 0.04 2.82<br />
2476 RB4458 342 1029 Protein of unknown function, UPF0118 2287089 2286061 6.4 37001 - 6.31 -1.04 0.18 17.78<br />
2 RB2 59 180 secreted protein 182 3 3.7 6489 - 6.80 -2.66 0.14 5.09<br />
7 RB13 130 393 hypothetical protein 5850 5458 11 14731 - 6.36 -1.07 0.11 10.71<br />
22 RB37 160 483 membrane protein 18328 17846 5.6 17283 - 6.08 -1.02 0.17 16.27<br />
34 RB56 89 270 hypothetical protein 24833 24564 4.5 9645 - 5.93 -1.08 0.11 10.14<br />
37 RB61 58 177 hypothetical protein 25378 25554 9.6 6492 + 6.04 -1.13 0.10 8.49<br />
130 RB229 585 1758 secreted protein containing DUF1551 122782 121025 4.5 62454 - 6.09 -1.09 0.06 5.70<br />
182 RB319 95 288 hypothetical protein 159334 159621 10.2 10505 + 6.75 -1.56 0.11 7.20<br />
193 RB337 97 294 hypothetical protein 169241 169534 12.5 11059 + 6.03 -1.00 0.12 11.56<br />
202 RB354 51 156 hypothetical protein 177535 177380 12 5703 - 6.09 -1.15 0.13 11.71<br />
209 RB367 67 204 hypothetical protein 183924 183721 10.4 7521 - 5.89 -1.02 0.10 9.43<br />
250 RB434 65 198 hypothetical protein 231791 231988 7.4 7385 + 6.41 -1.41 0.12 8.33<br />
409 RB767 311 936 conserved hypothetical protein, secreted 411420 412355 5.7 34002 + 5.78 -1.00 0.16 16.12<br />
516 RB951 234 705 protein containing DUF1596 502459 501755 12.3 25583 - 6.30 -1.47 0.13 8.81<br />
622 RB1179 128 387 hypothetical protein 603998 604384 12 13863 + 7.02 -1.09 0.01 1.26<br />
1067 RB2034 155 468 hypothetical protein 1071018 1071485 10.5 17610 + 6.82 -1.01 0.01 0.84<br />
1088 RB2080 46 141 hypothetical protein 1091549 1091409 9.9 5071 - 6.76 -1.56 0.04 2.41<br />
1105 RB2105 470 1413 membrane protein 1114012 1112600 9.6 51853 - 6.06 -1.14 0.12 10.13<br />
1130 RB2150 93 282 hypothetical protein 1138311 1138030 4.4 10755 - 7.08 -2.06 0.14 6.60<br />
1183 RB2247 579 1740 conserved hypothetical protein containing YTV domains 1181238 1179499 9.8 65013 - 7.18 -1.67 0.06 3.29<br />
1184 RB2251 75 228 hypothetical protein 1181714 1181487 4.5 8027 - 7.30 -1.84 0.07 3.97<br />
1188 RB2255 628 1887 hypothetical protein 1186018 1184132 4.1 66330 - 6.72 -1.58 0.06 3.72<br />
1217 RB2302 46 141 hypothetical protein 1204388 1204528 5.6 5335 + 6.85 -1.29 0.12 9.67<br />
1277 RB2384 65 198 hypothetical protein 1242697 1242500 12 7263 - 6.41 -1.27 0.08 6.52<br />
1352 RB2503 515 1548 O-antigen flippase wzx 1311056 1309509 10 55740 - 5.93 -1.07 0.25 22.97<br />
1353 RB2505 82 249 hypothetical protein 1311059 1311307 10 9658 + 6.06 -1.06 0.20 18.98<br />
1424 RB2628 43 132 hypothetical protein 1365707 1365576 10 4777 - 6.35 -1.39 0.05 3.72<br />
1428 RB2636 116 351 hypothetical protein 1368450 1368100 11.7 12761 - 6.88 -1.54 0.02 1.11<br />
1446 RB2667 236 711 membrane protein 1387198 1386488 10.7 25698 - 6.10 -1.11 0.20 17.74<br />
1479 RB2726 56 171 hypothetical protein 1421143 1421313 10.5 6492 + 6.25 -1.02 0.07 7.34<br />
1557 RB2883 120 363 hypothetical protein 1499497 1499859 11.8 13089 + 6.09 -1.09 0.23 21.41<br />
1560 RB2886 118 357 membrane protein 1501522 1501878 7.6 13062 + 6.20 -1.02 0.12 11.94<br />
1597 RB2951 174 525 membrane protein 1523616 1523092 9.3 20061 - 6.02 -1.02 0.16 15.54<br />
1684 RB3117 89 270 hypothetical protein 1615433 1615164 11.2 9951 - 6.20 -1.28 0.27 20.89<br />
1692 RB3132 112 339 membrane protein 1619400 1619062 10.3 12831 - 5.98 -1.01 0.13 12.47<br />
1705 RB3152 68 207 hypothetical protein 1626667 1626461 12.2 7261 - 6.51 -1.21 0.04 3.21<br />
1771 RB3268 231 696 hypothetical protein 1683003 1682308 12 25730 - 6.12 -1.11 0.16 14.69<br />
1776 RB3277 221 666 hypothetical protein 1686552 1685887 10.4 24222 - 6.05 -1.26 0.14 11.34<br />
1789 RB3301 50 153 hypothetical protein 1694208 1694056 10.5 5470 - 6.22 -1.25 0.11 8.64<br />
1819 RB3353 409 1230 Sialidase 1 Neu1 3.2.1.18 1725519 1724290 5.9 45043 - 5.98 -1.07 0.11 10.22<br />
1825 RB3362 87 264 hypothetical protein 1728546 1728283 11.9 10017 - 6.17 -1.29 0.14 10.53<br />
1827 RB3365 43 132 hypothetical protein 1730124 1730255 11.4 4886 + 8.16 -1.80 0.12 6.58<br />
1828 RB3366 78 237 hypothetical protein 1730380 1730616 5.6 9481 + 6.11 -1.24 0.03 2.58<br />
1833 RB3372 115 348 hypothetical protein 1733666 1734013 10 12916 + 6.02 -1.06 0.12 11.38<br />
1843 RB3393 60 183 secreted protein 1751865 1752047 12.3 7156 + 6.78 -1.29 0.06 4.38<br />
1844 RB3394 36 111 hypothetical protein 1752044 1752154 10.5 4239 + 6.34 -1.41 0.10 7.06<br />
1845 RB3395 398 1197 secreted protein containing DUF1559 1752151 1753347 7.8 43136 + 6.62 -1.09 0.10 8.85<br />
1847 RB3399 65 198 hypothetical protein 1753650 1753453 9.7 7418 - 6.22 -1.51 0.16 10.33<br />
1943 RB3566 64 195 hypothetical protein 1854080 1854274 9.4 7400 + 6.08 -1.18 0.23 19.64<br />
1950 RB3575 152 459 membrane protein 1858779 1859237 10.1 17195 + 6.12 -1.43 0.15 10.39<br />
1992 RB3644 127 384 hypothetical protein 1895184 1894801 4.9 14294 - 7.24 -1.78 0.10 5.75<br />
2006 RB3660 94 285 hypothetical protein 1900854 1900570 9.4 10411 - 6.33 -1.07 0.11 10.22<br />
2011 RB3665 119 360 hypothetical protein 1903076 1902717 5.9 12608 - 6.05 -1.09 0.17 15.75<br />
2017 RB3675 742 2229 secreted protein 1904552 1906780 8.4 82951 + 6.11 -1.11 0.10 9.30<br />
2025 RB3688 53 162 hypothetical protein 1910629 1910468 9.3 5981 - 6.19 -1.24 0.09 7.17<br />
2048 RB3719 141 426 hypothetical protein 1927500 1927075 10.8 16330 - 6.54 -1.52 0.15 10.13<br />
2113 RB3835 50 153 hypothetical protein 1989687 1989839 11.3 5789 + 6.12 -1.04 0.18 17.53<br />
2124 RB3851 75 228 hypothetical protein 1996660 1996433 7.6 8884 - 6.15 -1.02 0.17 16.90<br />
2146 RB3890 125 378 hypothetical protein 2011554 2011177 4.4 14580 - 6.17 -1.17 0.05 3.93<br />
2154 RB3905 38 117 hypothetical protein 2018601 2018717 5.1 4131 + 6.23 -1.19 0.06 4.95<br />
2174 RB3935 55 168 hypothetical protein 2036241 2036074 9.3 5982 - 6.25 -1.01 0.11 11.09<br />
2184 RB3953 857 2574 hypothetical protein 2048784 2046211 5.2 93881 - 6.11 -1.29 0.27 21.35<br />
2198 RB3975 204 615 hypothetical protein 2056726 2057340 12.3 22602 + 6.10 -1.27 0.07 5.13<br />
2200 RB3981 161 486 hypothetical protein 2058000 2058485 4.1 18283 + 6.04 -1.09 0.04 4.05<br />
2210 RB3994 191 576 hypothetical protein 2061291 2061866 4.1 20739 + 6.02 -1.15 0.22 18.97<br />
2258 RB4093 45 138 hypothetical protein 2107600 2107737 10 5285 + 6.08 -1.09 0.14 12.53<br />
2260 RB4097 733 2202 conserved hypothetical protein 2108244 2110445 6.2 83188 + 6.12 -1.23 0.09 7.05<br />
2285 RB4142 43 132 hypothetical protein 2130009 2130140 12 4964 + 6.28 -1.00 0.10 10.12<br />
2287 RB4145 90 273 hypothetical protein 2131933 2131661 12 10727 - 6.49 -1.09 0.11 10.21<br />
2312 RB4194 53 162 hypothetical protein 2156685 2156524 11.4 5728 - 7.23 -1.35 0.09 6.79<br />
2323 RB4210 148 447 hypothetical protein 2160917 2161363 6.5 16112 + 6.18 -1.15 0.09 8.11<br />
2343 RB4243 58 177 hypothetical protein 2174155 2174331 4.4 6442 + 6.01 -1.00 0.12 11.83<br />
2356 RB4264 120 363 membrane protein 2183157 2182795 10.7 13335 - 6.28 -1.27 0.16 12.53<br />
2368 RB4282 291 876 conserved hypothetical protein 2199894 2199019 5.3 33373 - 6.08 -1.08 0.19 17.49<br />
2369 RB4283 499 1500 PvdD/PvdJ 2201462 2199963 5.7 56411 - 6.04 -1.26 0.08 6.05<br />
2371 RB4285 841 2526 membrane protein 2204136 2201611 5.9 93852 - 6.03 -1.07 0.18 17.10<br />
2378 RB4296 240 723 hypothetical protein 2213931 2213209 5.8 27191 - 6.11 -1.23 0.14 11.24<br />
2416 RB4358 123 372 hypothetical protein 2239474 2239103 6.5 13459 - 6.19 -1.22 0.09 7.78<br />
2417 RB4360 156 471 hypothetical protein 2240075 2239605 4.7 17592 - 6.16 -1.09 0.12 11.00<br />
2424 RB4373 109 330 hypothetical protein 2242863 2242534 4.8 12263 - 6.12 -1.07 0.18 17.17<br />
2593 RB4652 52 159 hypothetical protein 2380494 2380652 12.6 5628 + 6.91 -1.20 0.13 10.54<br />
2596 RB4657 123 372 hypothetical protein 2381865 2382236 12.2 13398 + 6.29 -1.87 0.18 9.70<br />
2647 RB4740 50 153 hypothetical protein 2431814 2431966 11.4 5598 + 6.17 -1.15 0.08 6.72<br />
2746 RB4897 184 555 hypothetical protein 2519505 2518951 10.9 20446 - 5.85 -1.00 0.14 13.88<br />
2777 RB4941 173 522 hypothetical protein 2541619 2542140 11.2 19306 + 6.00 -1.51 0.04 2.76<br />
2781 RB4951 95 288 hypothetical protein 2547498 2547785 12.1 10953 + 6.73 -1.38 0.09 6.53<br />
2964 RB5262 95 288 membrane protein 2713742 2714029 6.3 10993 + 6.04 -1.27 0.10 8.24<br />
3045 RB5409 97 294 hypothetical protein 2794195 2794488 12.7 10898 + 8.04 -1.98 0.08 3.83<br />
3049 RB5415 62 189 hypothetical protein 2798737 2798925 12.3 7098 + 6.86 -1.51 0.11 7.26<br />
3052 RB5418 73 222 secreted protein 2805185 2804964 11 8406 - 5.85 -1.06 0.09 8.57
3106 RB5505 73 222 hypothetical protein 2850866 2851087 12.9 8233 + 6.40 -1.06 0.13 12.23<br />
3132 RB5555 54 165 hypothetical protein 2891274 2891438 6.2 5971 + 6.10 -1.11 0.07 6.00<br />
3239 RB5745 130 393 hypothetical protein 3001234 3000842 10.7 13522 - 7.50 -1.89 0.07 3.46<br />
3271 RB5802 156 471 hypothetical protein 3039864 3040334 10.1 17001 + 7.00 -1.34 0.13 9.54<br />
3466 RB6158 142 429 hypothetical protein 3233532 3233104 6 15746 - 6.20 -1.51 0.19 12.53<br />
3476 RB6174 69 210 hypothetical protein 3239734 3239525 10.6 7870 - 5.95 -1.14 0.19 16.98<br />
3485 RB6193 62 189 hypothetical protein 3246096 3245908 11.4 6970 - 6.39 -1.51 0.17 11.02<br />
3547 RB6314 52 159 hypothetical protein 3319320 3319478 6.3 6180 + 6.96 -2.29 0.17 7.47<br />
3617 RB6423 45 138 hypothetical protein 3380603 3380740 5.5 5402 + 6.58 -1.29 0.06 4.69<br />
3701 RB6553 74 225 hypothetical protein 3479627 3479403 12.1 8987 - 6.37 -1.18 0.03 2.27<br />
3731 RB6609 45 138 hypothetical protein 3511456 3511593 10.4 5004 + 6.66 -1.10 0.17 15.59<br />
3744 RB6634 365 1098 protein containing DUF1559 3525785 3526882 5.3 40217 + 5.88 -1.17 0.14 11.55<br />
3836 RB6789 180 543 secreted protein 3608487 3607945 5 20191 - 6.06 -1.32 0.09 6.71<br />
3867 RB6849 101 306 hypothetical protein 3642537 3642232 12.8 12002 - 6.08 -1.12 0.12 11.06<br />
3971 RB7042 91 276 hypothetical protein 3743971 3744246 10.4 10835 + 5.77 -1.08 0.05 5.10<br />
4015 RB7116 59 180 hypothetical protein 3779586 3779765 11.7 6458 + 7.14 -1.37 0.06 4.61<br />
4016 RB7117 181 546 Ribosomal protein L35 3780322 3779777 11.4 19816 - 7.00 -1.48 0.05 3.63<br />
4049 RB7178 45 138 hypothetical protein 3806924 3807061 12.3 5320 + 7.17 -1.64 0.11 6.85<br />
4050 RB7180 104 315 hypothetical protein 3807345 3807031 10.8 11535 - 6.84 -1.50 0.04 2.52<br />
4051 RB7184 190 573 conserved hypothetical protein 3807517 3808089 9.2 21306 + 6.51 -1.58 0.05 3.36<br />
4092 RB7246 50 153 hypothetical protein 3850393 3850545 9.7 5973 + 6.82 -1.40 0.02 1.51<br />
4131 RB7313 51 156 hypothetical protein 3892097 3891942 10.5 5558 - 7.82 -1.61 0.10 6.10<br />
4200 RB7455 838 2517 conserved hypothetical protein containing YTV domains 3999277 3996761 9 92217 - 6.93 -1.24 0.12 9.32<br />
4237 RB7530 97 294 hypothetical protein 4049298 4049005 12.4 11656 - 6.39 -2.97 0.12 4.05<br />
4241 RB7535 136 411 hypothetical protein 4050024 4050434 10.9 15058 + 6.90 -1.48 0.03 2.20<br />
4278 RB7589 86 261 hypothetical protein 4080238 4079978 12.1 9472 - 5.65 -1.15 0.08 6.98<br />
4314 RB7646 62 189 hypothetical protein 4111735 4111923 10.5 6950 + 6.91 -1.63 0.05 2.97<br />
4315 RB7647 73 222 hypothetical protein 4111931 4112152 7.4 8269 + 9.28 -1.65 0.07 4.03<br />
4327 RB7667 38 117 hypothetical protein 4117097 4116981 6.7 4315 - 7.05 -1.41 0.05 3.68<br />
4424 RB7832 50 153 hypothetical protein 4192499 4192651 13 5566 + 7.71 -1.16 0.04 3.14<br />
4486 RB7939 105 318 hypothetical protein 4238296 4238613 11 12019 + 6.22 -1.07 0.20 18.69<br />
4531 RB8034 147 444 membrane protein 4295344 4295787 10.2 16546 + 5.87 -1.31 0.25 19.23<br />
4568 RB8098 71 216 hypothetical protein 4337334 4337119 10.8 7950 - 9.46 -1.09 0.21 19.10<br />
4696 RB8353 82 249 hypothetical protein 4459374 4459622 10.8 9402 + 6.33 -2.57 0.19 7.30<br />
4861 RB8669 37 114 hypothetical protein 4614557 4614670 11.5 4395 + 9.56 -1.08 0.21 19.04<br />
5052 RB9020 381 1146 secreted protein 4807486 4808631 5.9 42557 + 6.74 -1.05 0.14 13.65<br />
5068 RB9057 95 288 hypothetical protein 4834849 4834562 10.9 10885 - 7.16 -1.08 0.09 7.96<br />
5072 RB9067 57 174 secreted protein 4839661 4839834 3.4 6172 + 6.09 -1.04 0.08 7.66<br />
5205 RB9324 267 804 secreted protein 4992474 4993277 6.7 29463 + 5.61 -1.01 0.09 9.16<br />
5214 RB9343 59 180 hypothetical protein 5003789 5003968 11.4 6521 + 6.09 -1.03 0.18 17.37<br />
5280 RB9460 79 240 hypothetical protein 5078860 5078621 10.3 8991 - 9.22 -1.24 0.10 8.44<br />
5694 RB10209 110 333 H+-transporting two-sector ATPase, C subunit 3.6.3.14 5515135 5515467 10.2 11565 + 6.92 -1.07 0.05 5.04<br />
5838 RB10483 133 402 conserved hypothetical protein 5685437 5685838 5.7 14433 + 6.30 -1.38 0.20 14.14<br />
5855 RB10521 53 162 hypothetical protein 5708182 5708343 9.1 6074 + 6.72 -1.29 0.06 4.68<br />
5887 RB10581 384 1155 secreted protein containing DUF1559 5739353 5740507 6.2 41431 + 7.21 -1.07 0.06 5.42<br />
6364 RB11429 37 114 conserved hypothetical protein 6196478 6196591 12.4 4245 + 6.94 -1.40 0.10 6.98<br />
6371 RB11439 93 282 conserved hypothetical protein 6197993 6198274 10.6 10906 + 8.15 -2.53 0.05 1.90<br />
6385 RB11450 60 183 conserved hypothetical protein 6200046 6200228 11.8 6517 + 8.71 -1.58 0.09 5.52<br />
6697 RB12021 82 249 conserved hypothetical protein 6485018 6484770 11.9 9305 - 6.37 -1.06 0.15 14.50<br />
6867 RB12329 110 333 conserved hypothetical protein, membrane 6634234 6634566 4 11862 + 7.08 -1.18 0.04 3.05<br />
7046 RB12649 112 339 conserved hypothetical protein 6810876 6810538 10.3 12615 - 5.53 -1.01 0.03 3.06<br />
7141 RB12818 163 492 conserved hypothetical protein 6915989 6915498 11.7 18010 - 7.62 -1.82 0.09 4.79<br />
20min ID Locus AA Nuc Product Gene EC Start Stop IEP MW(kDa) Strand Intensity Ratio Stdev %<br />
4829 RB8592 233 702 conserved hypothetical protein 4580947 4581648 6 26322 + 6.52 -1.14 0.03 3.05<br />
6656 RB11945 556 1671 aldehyde dehydrogenase family protein 6434111 6432441 10.9 59626 - 6.66 -1.28 0.04 3.31<br />
515 RB950 442 1329 Cationic amino acid transporter 501765 500437 9.7 46180 - 6.31 -1.01 0.02 2.34<br />
1967 RB3603 344 1035 secreted protein 1875461 1874427 4.6 37999 - 6.21 -1.02 0.05 4.86<br />
2359 RB4269 282 849 glutamic acid specific endopeptidase, GSE=serine endopeptidase 2185049 2184201 5.6 31283 - 6.27 -1.09 0.10 9.05<br />
6865 RB12327 686 2061 TGF-beta receptor, type I/II extracellular region 6633918 6631858 4.5 75645 - 6.39 -1.69 0.08 4.89<br />
4927 RB8785 542 1629 L-fucose isomerase, 4672793 4671165 4.8 59809 - 6.31 -1.16 0.14 11.69<br />
6891 RB12379 240 723 riboflavin synthase, alpha subunit 6662808 6662086 4.6 25728 - 6.56 -1.28 0.24 18.56<br />
179 RB314 309 930 malonyl CoA-acyl carrier protein transacylase fabD 2.3.1.39 156968 157897 4.4 32349 + 7.20 -2.10 0.36 16.94<br />
578 RB1092 396 1191 3-hydroxybutyryl-CoA dehydrogenase 565978 567168 5.2 43249 + 6.20 -1.01 0.09 8.93<br />
2733 RB4876 397 1194 delta 9 acyl-lipid fatty acid desaturase 1.14.19.- 2506214 2505021 9.5 44741 - 7.98 -1.17 0.13 10.86<br />
658 RB1233 206 621 30S ribosomal protein S4 rpsD 629490 628870 11.2 23193 - 7.15 -1.31 0.10 7.41<br />
4423 RB7829 108 327 Ribosomal protein S10 4192031 4192357 10.3 11931 + 7.67 -1.07 0.08 7.47<br />
4427 RB7836 107 324 Ribosomal L23 protein 4194060 4194383 10.8 12651 + 7.53 -1.27 0.10 7.67<br />
4428 RB7837 286 861 Ribosomal protein L2 4194481 4195341 11.8 31108 + 7.08 -1.25 0.27 21.40<br />
4750 RB8459 55 168 30S ribosomal protein S18 rpsR 4518496 4518663 10.5 6203 + 7.33 -1.37 0.26 19.17<br />
4824 RB8580 113 342 sigma-54 modulation protein 4577393 4577734 5.7 12706 + 7.05 -1.09 0.18 16.81<br />
5832 RB10471 151 456 Ribosomal protein L13 5678759 5678304 10 17369 - 7.61 -1.45 0.09 6.14<br />
7029 RB12623 127 384 Ribosomal protein S13 6796904 6797287 10.8 14481 + 7.38 -1.35 0.14 10.10<br />
7030 RB12625 127 384 Ribosomal protein S11 6797354 6797737 11.4 13570 + 7.24 -1.04 0.15 14.01<br />
7143 RB12821 117 354 Ribosomal protein L19 6916558 6916205 11.1 13347 - 6.83 -1.55 0.31 19.78<br />
7145 RB12824 146 441 Ribosomal protein S16 6917743 6917303 5.3 15885 - 7.14 -1.54 0.28 18.09<br />
4459 RB7894 398 1197 translation elongation factor EF-Tu 4217484 4218680 5.2 43184 + 7.57 -2.56 0.09 3.64<br />
2610 RB4681 65 198 cold shock protein cspA 2391934 2392131 4.7 6982 + 7.32 -2.75 0.32 11.59<br />
6819 RB12251 567 1704 DNA-directed RNA polymerase specialized sigma factor 6599262 6597559 9.4 59396 - 6.38 -1.06 0.16 14.82<br />
3525 RB6271 325 978 Tyrosine recombinase xerD xerD 3294814 3295791 10 36623 + 6.38 -1.34 0.11 8.16<br />
3528 RB6276 105 318 Histone-like bacterial DNA-binding protein 3297504 3297187 10.4 11299 - 8.02 -2.23 0.13 5.82<br />
5531 RB9917 169 510 Single-strand binding protein/Primosomal replication protein n ssb 5344370 5343861 5.3 18108 - 7.18 -1.36 0.26 19.07<br />
6885 RB12363 787 2364 UvrD/REP helicase 6656202 6653839 5.3 88835 - 6.49 -1.10 0.13 11.87<br />
3524 RB6269 405 1218 UDP-glucose 4-epimerase galE 5.1.3.2 3294798 3293581 5.3 44117 - 6.21 -1.21 0.17 14.13<br />
6968 RB12502 435 1308 flagellar motor switch protein FliG fliG 6732844 6731537 7 47011 - 6.35 -1.12 0.24 21.20<br />
5282 RB9463 628 1887 ATPase with chaperone activity 5079098 5080984 9.4 69732 + 6.25 -1.07 0.18 16.75<br />
642 RB1205 456 1371 choline sulfatase betC 3.1.6.6 615037 613667 6.6 51848 - 6.31 -1.26 0.14 10.88<br />
7313 RB13148 1012 3039 arylsulfatase A [precursor] 7058006 7054968 6.2 111097 - 6.37 -1.37 0.13 9.12<br />
6663 RB11958 302 909 protein containing DUF191 6443018 6442110 4.5 32855 - 6.58 -1.41 0.16 11.59<br />
6983 RB12532 405 1218 metallophosphoesterase, secreted 6749031 6750248 5.8 45205 + 6.34 -1.32 0.21 15.86<br />
6686 RB11998 412 1239 secreted protein containing DUF323 6474041 6472803 4.1 45898 - 6.66 -1.18 0.14 12.18<br />
4269 RB7578 189 570 Carbon storage regulator csrA 4070466 4071035 8.8 20421 + 7.23 -1.77 0.12 7.02<br />
5722 RB10261 81 246 Carbon storage regulator csrA 5545964 5545719 5.1 8704 - 7.91 -1.11 0.07 6.27<br />
6680 RB11988 880 2643 serine/threonine-protein kinase spk1 2.7.1.37 6468163 6465521 5.4 97920 - 6.34 -1.04 0.09 8.90<br />
6776 RB12183 585 1758 GGDEF family protein 6573556 6571799 4.7 63291 - 6.28 -1.02 0.23 22.47<br />
2 RB2 59 180 secreted protein 182 3 3.7 6489 - 7.11 -3.11 0.58 18.48<br />
37 RB61 58 177 hypothetical protein 25378 25554 9.6 6492 + 6.26 -1.18 0.08 7.01<br />
449 RB825 117 354 hypothetical protein 434184 433831 7.8 13302 - 6.17 -1.01 0.18 17.58<br />
516 RB951 234 705 protein containing DUF1596 502459 501755 12.3 25583 - 6.53 -1.37 0.22 16.44<br />
622 RB1179 128 387 hypothetical protein 603998 604384 12 13863 + 7.35 -1.12 0.14 12.47<br />
655 RB1230 117 354 hypothetical protein 627328 626975 11.7 13093 - 6.73 -1.38 0.21 15.60<br />
686 RB1281 230 693 hypothetical protein 653595 654287 10.5 25350 + 6.33 -1.09 0.09 8.05<br />
691 RB1291 97 294 hypothetical protein 656684 656977 10.4 10290 + 6.24 -1.16 0.17 14.49<br />
980 RB1860 110 333 hypothetical protein 960361 960693 10.9 11827 + 6.37 -1.26 0.02 1.63<br />
1130 RB2150 93 282 hypothetical protein 1138311 1138030 4.4 10755 - 7.18 -1.92 0.39 20.42<br />
1183 RB2247 579 1740 conserved hypothetical protein containing YTV domains 1181238 1179499 9.8 65013 - 7.17 -1.77 0.32 17.87<br />
1184 RB2251 75 228 hypothetical protein 1181714 1181487 4.5 8027 - 7.28 -1.64 0.35 21.14<br />
1827 RB3365 43 132 hypothetical protein 1730124 1730255 11.4 4886 + 8.48 -2.19 0.25 11.34<br />
2312 RB4194 53 162 hypothetical protein 2156685 2156524 11.4 5728 - 7.53 -1.11 0.12 10.86<br />
2593 RB4652 52 159 hypothetical protein 2380494 2380652 12.6 5628 + 7.03 -1.34 0.11 8.02<br />
2596 RB4657 123 372 hypothetical protein 2381865 2382236 12.2 13398 + 6.52 -1.59 0.14 8.88<br />
3045 RB5409 97 294 hypothetical protein 2794195 2794488 12.7 10898 + 7.61 -2.73 0.08 2.75<br />
3239 RB5745 130 393 hypothetical protein 3001234 3000842 10.7 13522 - 7.29 -2.96 0.24 8.02<br />
3356 RB5957 154 465 secreted protein 3122093 3122557 3.3 15514 + 7.51 -1.32 0.12 8.86<br />
3476 RB6174 69 210 hypothetical protein 3239734 3239525 10.6 7870 - 6.31 -1.18 0.14 12.01<br />
3485 RB6193 62 189 hypothetical protein 3246096 3245908 11.4 6970 - 6.43 -1.21 0.15 12.23<br />
3527 RB6274 59 180 hypothetical protein 3297130 3296951 12 7254 - 6.31 -1.14 0.12 10.58<br />
3725 RB6594 295 888 hypothetical protein 3505472 3506359 10.2 32951 + 6.33 -1.42 0.13 9.46<br />
3731 RB6609 45 138 hypothetical protein 3511456 3511593 10.4 5004 + 6.73 -1.12 0.15 13.00<br />
3733 RB6615 230 693 secreted protein containing DUF1080 3515430 3516122 5.1 25770 + 6.54 -1.24 0.13 10.83<br />
3826 RB6777 137 414 hypothetical protein 3602786 3603199 11.5 15512 + 7.41 -1.07 0.10 9.24<br />
3836 RB6789 180 543 secreted protein 3608487 3607945 5 20191 - 6.43 -1.36 0.09 6.62<br />
3901 RB6911 430 1293 protein containing DUF1559 3671445 3672737 6 45752 + 6.46 -1.25 0.21 17.02
4008 RB7108 91 276 hypothetical protein 3775303 3775578 10.9 10200 + 6.34 -1.08 0.20 18.94<br />
4016 RB7117 181 546 Ribosomal protein L35 3780322 3779777 11.4 19816 - 7.07 -1.85 0.24 12.90<br />
4049 RB7178 45 138 hypothetical protein 3806924 3807061 12.3 5320 + 7.04 -1.96 0.42 21.20<br />
4050 RB7180 104 315 hypothetical protein 3807345 3807031 10.8 11535 - 7.15 -1.84 0.35 18.78<br />
4131 RB7313 51 156 hypothetical protein 3892097 3891942 10.5 5558 - 7.28 -1.90 0.25 13.08<br />
4175 RB7414 108 327 hypothetical protein 3965263 3964937 12.2 11737 - 6.50 -1.18 0.07 6.09<br />
4241 RB7535 136 411 hypothetical protein 4050024 4050434 10.9 15058 + 6.69 -1.38 0.06 4.06<br />
4315 RB7647 73 222 hypothetical protein 4111931 4112152 7.4 8269 + 8.82 -2.20 0.36 16.49<br />
4377 RB7748 74 225 hypothetical protein 4150491 4150715 8.9 8433 + 6.33 -1.00 0.20 20.49<br />
4424 RB7832 50 153 hypothetical protein 4192499 4192651 13 5566 + 7.71 -1.38 0.05 3.87<br />
4647 RB8258 136 411 hypothetical protein 4414716 4415126 12.4 15387 + 6.50 -1.00 0.07 6.80<br />
4861 RB8669 37 114 hypothetical protein 4614557 4614670 11.5 4395 + 9.00 -2.37 0.38 15.90<br />
4952 RB8833 317 954 Zinc transporter ZIP 4696475 4697428 6.8 33766 + 6.29 -1.08 0.09 8.70<br />
4971 RB8866 445 1338 secreted conserved hypothetical protein 4716964 4718301 6 49999 + 6.44 -1.58 0.21 13.22<br />
4976 RB8876 87 264 hypothetical protein 4723629 4723892 9.5 9540 + 6.56 -1.22 0.15 12.29<br />
5052 RB9020 381 1146 secreted protein 4807486 4808631 5.9 42557 + 6.89 -1.12 0.06 5.70<br />
5068 RB9057 95 288 hypothetical protein 4834849 4834562 10.9 10885 - 7.17 -1.13 0.08 7.12<br />
5074 RB9070 409 1230 secreted protein containing DUF1559 4840267 4841496 8.6 44198 + 5.95 -1.11 0.00 0.40<br />
5114 RB9142 131 396 hypothetical protein 4892884 4893279 11.3 14259 + 6.64 -1.30 0.20 15.27<br />
5204 RB9322 42 129 hypothetical protein 4992459 4992331 7.3 4319 - 6.31 -1.38 0.10 7.07<br />
5205 RB9324 267 804 secreted protein 4992474 4993277 6.7 29463 + 6.44 -1.34 0.27 19.86<br />
5214 RB9343 59 180 hypothetical protein 5003789 5003968 11.4 6521 + 6.27 -1.32 0.15 11.16<br />
5258 RB9417 103 312 hypothetical protein 5050621 5050932 10.5 11390 + 6.48 -1.60 0.19 11.92<br />
5280 RB9460 79 240 hypothetical protein 5078860 5078621 10.3 8991 - 8.75 -2.03 0.27 13.57<br />
5283 RB9469 409 1230 membrane protein 5081396 5082625 4.3 44608 + 6.50 -1.19 0.10 8.70<br />
5300 RB9496 174 525 hypothetical protein 5098535 5099059 11 19521 + 6.58 -1.40 0.11 7.92<br />
5304 RB9501 169 510 conserved hypothetical protein 5102922 5103431 5.6 18781 + 6.48 -1.27 0.24 18.72<br />
5318 RB9529 113 342 hypothetical protein 5117956 5118297 9.3 13082 + 6.36 -1.28 0.22 17.49<br />
5371 RB9624 357 1074 hypothetical protein 5181259 5180186 5.2 38849 - 6.17 -1.07 0.01 1.30<br />
5488 RB9844 317 954 cytochrome c biogenesis protein, CcmF/CcyK/CcsA family 5301844 5302797 9.4 34187 + 6.27 -1.01 0.03 3.27<br />
5503 RB9872 67 204 hypothetical protein 5316692 5316895 5.4 6912 + 6.59 -1.61 0.11 7.01<br />
5583 RB10021 48 147 hypothetical protein 5396777 5396923 11.4 5300 + 6.32 -1.05 0.22 21.01<br />
5855 RB10521 53 162 hypothetical protein 5708182 5708343 9.1 6074 + 6.73 -1.25 0.25 20.19<br />
5885 RB10579 101 306 secreted protein 5739254 5738949 12.4 11507 - 7.24 -1.48 0.24 16.05<br />
5954 RB10699 66 201 hypothetical protein 5812882 5813082 9.8 7157 + 6.51 -1.01 0.20 19.61<br />
6043 RB10861 46 141 hypothetical protein 5896851 5896991 4.2 4833 + 7.06 -1.00 0.14 13.55<br />
6046 RB10866 82 249 hypothetical protein 5899291 5899043 10.2 9214 - 6.57 -1.39 0.09 6.45<br />
6134 RB11019 77 234 hypothetical protein 5974374 5974141 9.1 8621 - 6.78 -1.17 0.09 8.12<br />
6232 RB11204 170 513 hypothetical protein 6077364 6076852 10.6 19111 - 6.41 -1.28 0.22 17.39<br />
6258 RB11245 53 162 hypothetical protein 6099126 6098965 6.3 6073 - 6.41 -1.39 0.27 19.50<br />
6280 RB11287 75 228 hypothetical protein 6118405 6118632 9.1 8478 + 6.25 -1.05 0.18 17.35<br />
6343 RB11392 148 447 conserved hypothetical protein 6177208 6177654 5 16382 + 6.50 -1.22 0.14 11.69<br />
6357 RB11416 363 1092 conserved hypothetical protein 6190901 6191992 5.2 41708 + 6.31 -1.06 0.04 4.13<br />
6371 RB11439 93 282 conserved hypothetical protein 6197993 6198274 10.6 10906 + 8.15 -2.35 0.26 11.05<br />
6385 RB11450 60 183 conserved hypothetical protein 6200046 6200228 11.8 6517 + 8.85 -1.77 0.40 22.47<br />
6416 RB11490 181 546 conserved hypothetical protein, membrane 6215558 6216103 10.3 20670 + 6.47 -1.25 0.05 4.39<br />
6557 RB11748 57 174 conserved hypothetical protein 6327844 6328017 5.8 6288 + 6.64 -1.25 0.06 4.96<br />
6569 RB11766 129 390 conserved hypothetical protein 6334355 6334744 10.4 14762 + 6.43 -1.48 0.21 14.44<br />
6579 RB11804 132 399 conserved hypothetical protein 6363537 6363139 11.3 15013 - 6.57 -1.83 0.21 11.54<br />
6580 RB11805 61 186 conserved hypothetical protein 6363715 6363530 12.1 7084 - 6.48 -1.87 0.16 8.33<br />
6652 RB11939 156 471 conserved hypothetical protein 6428116 6428586 11.9 17649 + 6.66 -1.13 0.10 8.71<br />
6683 RB11992 205 618 conserved hypothetical protein, membrane 6469784 6470401 5.8 22120 + 6.28 -1.04 0.13 12.59<br />
6697 RB12021 82 249 conserved hypothetical protein 6485018 6484770 11.9 9305 - 6.52 -1.14 0.14 12.54<br />
6781 RB12193 36 111 conserved hypothetical protein 6575977 6575867 7.5 3719 - 6.45 -1.50 0.04 2.56<br />
6787 RB12201 69 210 conserved hypothetical protein 6578161 6577952 12.5 7444 - 7.67 -1.09 0.04 3.81<br />
6801 RB12220 171 516 conserved hypothetical protein 6587027 6586512 4.7 19504 - 6.37 -1.24 0.02 1.34<br />
6803 RB12223 253 762 conserved hypothetical protein 6587469 6588230 10.1 27335 + 6.42 -1.34 0.10 7.09<br />
6822 RB12260 81 246 hypothetical protein-signal peptide prediction 6600777 6600532 11.9 9294 - 6.31 -1.24 0.04 3.46<br />
6857 RB12318 55 168 conserved hypothetical protein 6625565 6625398 11 6095 - 6.38 -1.55 0.06 3.95<br />
6936 RB12447 103 312 conserved hypothetical protein 6700272 6700583 10.6 11445 + 6.69 -2.14 0.16 7.70<br />
6941 RB12454 199 600 conserved hypothetical protein 6703647 6703048 10.3 22347 - 6.42 -1.44 0.15 10.71<br />
6960 RB12487 615 1848 conserved hypothetical protein 6724590 6722743 4.6 67294 - 6.42 -1.01 0.16 15.92<br />
6992 RB12550 63 192 conserved hypothetical protein 6762725 6762534 11.9 7124 - 6.24 -1.09 0.19 17.38<br />
6996 RB12557 41 126 conserved hypothetical protein 6765784 6765659 9.2 4684 - 6.39 -1.37 0.17 12.08<br />
7009 RB12581 174 525 conserved hypothetical protein 6780597 6780073 9.9 19433 - 6.33 -1.39 0.08 6.02<br />
7028 RB12621 85 258 conserved hypothetical protein 6796861 6796604 8.9 9777 - 6.39 -1.11 0.14 12.26<br />
7141 RB12818 163 492 conserved hypothetical protein 6915989 6915498 11.7 18010 - 7.32 -1.97 0.24 12.25<br />
7342 RB13207 58 177 secreted protein 7086761 7086585 11.9 6443 - 6.61 -1.44 0.19 13.11<br />
7365 RB13247 185 558 conserved hypothetical protein 7104177 7103620 10.2 21780 - 6.29 -1.33 0.12 8.68<br />
40min ID Locus AA Nuc Product Gene EC Start Stop IEP MW(kDa) Strand Intensity Ratio Stdev %<br />
5699 RB10217 479 1440 ATP synthase beta subunit 5519560 5520999 4.9 52151 + 5.98 -1.12 0.10 8.64<br />
6656 RB11945 556 1671 aldehyde dehydrogenase family protein 6434111 6432441 10.9 59626 - 6.42 -1.45 0.28 19.06<br />
2359 RB4269 282 849 glutamic acid specific endopeptidase, GSE=serine endopeptidase 2185049 2184201 5.6 31283 - 6.07 -1.10 0.11 10.00<br />
1423 RB2627 342 1029 Glyceraldehyde 3-phosphate dehydrogenase gap 1.2.1.12 1364551 1365579 5.4 36716 + 6.36 -1.30 0.09 6.85<br />
179 RB314 309 930 malonyl CoA-acyl carrier protein transacylase fabD 2.3.1.39 156968 157897 4.4 32349 + 6.72 -2.23 0.33 14.69<br />
2733 RB4876 397 1194 delta 9 acyl-lipid fatty acid desaturase 1.14.19.- 2506214 2505021 9.5 44741 - 7.20 -2.67 0.09 3.26<br />
658 RB1233 206 621 30S ribosomal protein S4 rpsD 629490 628870 11.2 23193 - 6.99 -1.29 0.08 6.04<br />
4413 RB7818 121 366 Ribosomal protein S12/S23 4185730 4186095 11.8 13642 + 7.54 -1.25 0.12 9.93<br />
4423 RB7829 108 327 Ribosomal protein S10 4192031 4192357 10.3 11931 + 7.09 -1.82 0.37 20.06<br />
4426 RB7834 222 669 Ribosomal protein L4/L1e 4193390 4194058 10.7 24288 + 6.77 -1.64 0.20 12.25<br />
4427 RB7836 107 324 Ribosomal L23 protein 4194060 4194383 10.8 12651 + 6.72 -2.01 0.11 5.67<br />
4428 RB7837 286 861 Ribosomal protein L2 4194481 4195341 11.8 31108 + 6.37 -1.42 0.12 8.70<br />
4431 RB7840 236 711 30S ribosomal protein S3 rpsC 4196161 4196871 10.4 26575 + 6.31 -1.38 0.22 16.24<br />
4441 RB7857 149 450 Ribosomal protein L18P/L5E 4200854 4201303 11.6 16541 + 6.16 -1.27 0.20 15.60<br />
4443 RB7863 164 495 Ribosomal protein L15 4202048 4202542 11.2 17961 + 5.84 -1.00 0.07 7.13<br />
4824 RB8580 113 342 sigma-54 modulation protein 4577393 4577734 5.7 12706 + 6.74 -1.13 0.07 6.34<br />
5832 RB10471 151 456 Ribosomal protein L13 5678759 5678304 10 17369 - 7.41 -1.83 0.22 11.77<br />
7029 RB12623 127 384 Ribosomal protein S13 6796904 6797287 10.8 14481 + 7.15 -1.38 0.13 9.55<br />
7030 RB12625 127 384 Ribosomal protein S11 6797354 6797737 11.4 13570 + 7.01 -1.23 0.12 9.96<br />
7143 RB12821 117 354 Ribosomal protein L19 6916558 6916205 11.1 13347 - 6.57 -1.29 0.05 3.48<br />
7145 RB12824 146 441 Ribosomal protein S16 6917743 6917303 5.3 15885 - 6.83 -1.46 0.02 1.40<br />
7154 RB12839 225 678 Ribosomal protein L1 6921934 6922611 9.8 24159 + 6.68 -1.33 0.29 21.38<br />
4459 RB7894 398 1197 translation elongation factor EF-Tu 4217484 4218680 5.2 43184 + 6.98 -2.77 0.15 5.45<br />
2610 RB4681 65 198 cold shock protein cspA 2391934 2392131 4.7 6982 + 7.07 -2.69 0.22 8.12<br />
3528 RB6276 105 318 Histone-like bacterial DNA-binding protein 3297504 3297187 10.4 11299 - 7.66 -3.21 0.14 4.44<br />
5531 RB9917 169 510 Single-strand binding protein/Primosomal replication protein n ssb 5344370 5343861 5.3 18108 - 6.81 -1.36 0.08 5.99<br />
6968 RB12502 435 1308 flagellar motor switch protein FliG fliG 6732844 6731537 7 47011 - 6.23 -1.15 0.15 13.30<br />
5282 RB9463 628 1887 ATPase with chaperone activity 5079098 5080984 9.4 69732 + 6.14 -1.03 0.19 18.18<br />
6983 RB12532 405 1218 metallophosphoesterase, secreted 6749031 6750248 5.8 45205 + 6.22 -1.33 0.18 13.36<br />
5228 RB9360 510 1533 Protein of unknown function, UPF0027 5015563 5014031 6.5 55186 - 6.52 -1.24 0.18 14.41<br />
6686 RB11998 412 1239 secreted protein containing DUF323 6474041 6472803 4.1 45898 - 6.64 -1.12 0.18 16.21<br />
4269 RB7578 189 570 Carbon storage regulator 4070466 4071035 8.8 20421 + 6.74 -1.74 0.28 16.14<br />
5722 RB10261 81 246 Carbon storage regulator csrA 5545964 5545719 5.1 8704 - 7.58 -1.88 0.16 8.54<br />
6776 RB12183 585 1758 GGDEF family protein 6573556 6571799 4.7 63291 - 6.16 -1.08 0.06 5.61<br />
182 RB319 95 288 hypothetical protein 159334 159621 10.2 10505 + 6.24 -1.18 0.09 7.43<br />
449 RB825 117 354 hypothetical protein 434184 433831 7.8 13302 - 6.15 -1.01 0.18 17.38<br />
516 RB951 234 705 protein containing DUF1596 502459 501755 12.3 25583 - 6.45 -1.59 0.18 11.29<br />
622 RB1179 128 387 hypothetical protein 603998 604384 12 13863 + 7.17 -1.11 0.03 2.42<br />
636 RB1199 181 546 hypothetical protein 611479 612024 5.7 19929 + 6.18 -1.11 0.24 21.96<br />
655 RB1230 117 354 hypothetical protein 627328 626975 11.7 13093 - 6.65 -1.26 0.20 15.90<br />
691 RB1291 97 294 hypothetical protein 656684 656977 10.4 10290 + 6.18 -1.09 0.06 5.35<br />
883 RB1644 123 372 secreted protein 845824 845453 9.7 13720 - 8.12 -2.49 0.04 1.44<br />
1099 RB2097 61 186 hypothetical protein 1106473 1106658 9.9 6506 + 6.01 -1.13 0.23 20.65<br />
1130 RB2150 93 282 hypothetical protein 1138311 1138030 4.4 10755 - 7.02 -1.73 0.21 12.33<br />
1183 RB2247 579 1740 conserved hypothetical protein containing YTV domains 1181238 1179499 9.8 65013 - 6.94 -1.54 0.09 5.56<br />
1184 RB2251 75 228 hypothetical protein 1181714 1181487 4.5 8027 - 6.89 -1.33 0.18 13.86<br />
1827 RB3365 43 132 hypothetical protein 1730124 1730255 11.4 4886 + 7.88 -3.89 0.12 3.04<br />
2312 RB4194 53 162 hypothetical protein 2156685 2156524 11.4 5728 - 7.18 -1.51 0.21 14.11<br />
2350 RB4255 204 615 hypothetical protein 2180806 2180192 10.3 21582 - 9.05 -1.31 0.04 3.10<br />
2553 RB4590 74 225 hypothetical protein 2352024 2351800 10.1 8330 - 9.49 -4.03 0.27 6.78<br />
2596 RB4657 123 372 hypothetical protein 2381865 2382236 12.2 13398 + 6.31 -1.61 0.31 19.08<br />
2789 RB4966 84 255 hypothetical protein 2552311 2552057 11.3 10016 - 9.27 -2.67 0.12 4.33
3045 RB5409 97 294 hypothetical protein 2794195 2794488 12.7 10898 + 7.26 -3.11 0.02 0.62<br />
3239 RB5745 130 393 hypothetical protein 3001234 3000842 10.7 13522 - 6.80 -3.11 0.16 5.28<br />
3258 RB5779 187 564 hypothetical protein 3028410 3028973 4.4 21341 + 7.09 -1.26 0.06 4.91<br />
3356 RB5957 154 465 secreted protein 3122093 3122557 3.3 15514 + 7.51 -1.19 0.15 12.61<br />
3547 RB6314 52 159 hypothetical protein 3319320 3319478 6.3 6180 + 6.35 -1.72 0.22 12.54<br />
3725 RB6594 295 888 hypothetical protein 3505472 3506359 10.2 32951 + 6.18 -1.37 0.06 4.44<br />
3733 RB6615 230 693 secreted protein containing DUF1080 3515430 3516122 5.1 25770 + 6.19 -1.19 0.13 11.02<br />
3826 RB6777 137 414 hypothetical protein 3602786 3603199 11.5 15512 + 7.26 -1.31 0.17 13.24<br />
3971 RB7042 91 276 hypothetical protein 3743971 3744246 10.4 10835 + 6.08 -1.09 0.19 17.91<br />
4015 RB7116 59 180 hypothetical protein 3779586 3779765 11.7 6458 + 6.75 -1.50 0.15 9.68<br />
4016 RB7117 181 546 Ribosomal protein L35 3780322 3779777 11.4 19816 - 6.86 -1.75 0.13 7.27<br />
4049 RB7178 45 138 hypothetical protein 3806924 3807061 12.3 5320 + 7.05 -1.53 0.24 15.72<br />
4050 RB7180 104 315 hypothetical protein 3807345 3807031 10.8 11535 - 7.19 -1.45 0.01 0.77<br />
4097 RB7253 59 180 hypothetical protein 3853668 3853847 9.7 6475 + 6.16 -1.07 0.01 1.17<br />
4126 RB7303 161 486 hypothetical protein 3883041 3883526 10.5 17533 + 6.12 -1.06 0.07 6.28<br />
4131 RB7313 51 156 hypothetical protein 3892097 3891942 10.5 5558 - 6.49 -2.26 0.18 7.97<br />
4175 RB7414 108 327 hypothetical protein 3965263 3964937 12.2 11737 - 6.25 -1.04 0.12 11.08<br />
4200 RB7455 838 2517 conserved hypothetical protein containing YTV domains 3999277 3996761 9 92217 - 6.16 -1.43 0.14 9.89<br />
4237 RB7530 97 294 hypothetical protein 4049298 4049005 12.4 11656 - 6.23 -1.80 0.22 12.22<br />
4314 RB7646 62 189 hypothetical protein 4111735 4111923 10.5 6950 + 6.24 -1.26 0.11 9.05<br />
4315 RB7647 73 222 hypothetical protein 4111931 4112152 7.4 8269 + 8.71 -2.99 0.12 3.94<br />
4377 RB7748 74 225 hypothetical protein 4150491 4150715 8.9 8433 + 6.23 -1.02 0.11 11.27<br />
4422 RB7828 40 123 hypothetical protein 4192037 4191915 10.4 4526 - 7.21 -1.38 0.13 9.18<br />
4424 RB7832 50 153 hypothetical protein 4192499 4192651 13 5566 + 7.19 -2.20 0.18 8.26<br />
4568 RB8098 71 216 hypothetical protein 4337334 4337119 10.8 7950 - 9.22 -3.48 0.14 4.07<br />
4589 RB8144 143 432 hypothetical protein 4354622 4354191 10.1 16269 - 8.38 -1.51 0.13 8.91<br />
4696 RB8353 82 249 hypothetical protein 4459374 4459622 10.8 9402 + 6.16 -1.64 0.08 5.04<br />
4861 RB8669 37 114 hypothetical protein 4614557 4614670 11.5 4395 + 8.49 -4.19 0.16 3.79<br />
4920 RB8776 86 261 hypothetical protein 4665512 4665772 9.3 9344 + 6.71 -1.14 0.19 16.87<br />
5052 RB9020 381 1146 secreted protein 4807486 4808631 5.9 42557 + 6.71 -1.06 0.09 8.90<br />
5068 RB9057 95 288 hypothetical protein 4834849 4834562 10.9 10885 - 6.88 -1.30 0.08 6.21<br />
5072 RB9067 57 174 secreted protein 4839661 4839834 3.4 6172 + 6.39 -1.07 0.17 15.41<br />
5108 RB9130 266 801 hypothetical protein 4886818 4886018 6.1 28140 - 6.35 -1.02 0.18 17.72<br />
5114 RB9142 131 396 hypothetical protein 4892884 4893279 11.3 14259 + 6.47 -1.15 0.22 18.80<br />
5204 RB9322 42 129 hypothetical protein 4992459 4992331 7.3 4319 - 6.25 -1.47 0.20 13.41<br />
5205 RB9324 267 804 secreted protein 4992474 4993277 6.7 29463 + 6.11 -1.14 0.05 4.37<br />
5225 RB9354 183 552 hypothetical protein 5011855 5012406 10.5 20002 + 6.18 -1.05 0.10 9.35<br />
5226 RB9356 129 390 protein containing DUF1579 5012771 5012382 9.2 14158 - 6.16 -1.84 0.25 13.35<br />
5258 RB9417 103 312 hypothetical protein 5050621 5050932 10.5 11390 + 6.28 -1.62 0.30 18.72<br />
5280 RB9460 79 240 hypothetical protein 5078860 5078621 10.3 8991 - 7.99 -4.04 0.14 3.40<br />
5300 RB9496 174 525 hypothetical protein 5098535 5099059 11 19521 + 6.30 -1.12 0.23 20.45<br />
5318 RB9529 113 342 hypothetical protein 5117956 5118297 9.3 13082 + 6.36 -1.58 0.31 19.61<br />
5503 RB9872 67 204 hypothetical protein 5316692 5316895 5.4 6912 + 6.37 -1.58 0.16 9.97<br />
5574 RB10005 77 234 hypothetical protein 5389186 5389419 4.4 8625 + 6.18 -1.04 0.07 7.05<br />
5810 RB10431 323 972 conserved hypothetical protein 5650397 5649426 10.1 36382 - 5.99 -1.10 0.11 9.80<br />
5838 RB10483 133 402 conserved hypothetical protein 5685437 5685838 5.7 14433 + 6.36 -1.34 0.23 17.28<br />
5855 RB10521 53 162 hypothetical protein 5708182 5708343 9.1 6074 + 6.51 -1.03 0.06 6.05<br />
5885 RB10579 101 306 secreted protein 5739254 5738949 12.4 11507 - 6.96 -1.50 0.10 6.43<br />
5887 RB10581 384 1155 secreted protein containing DUF1559 5739353 5740507 6.2 41431 + 6.75 -1.53 0.16 10.48<br />
6043 RB10861 46 141 hypothetical protein 5896851 5896991 4.2 4833 + 6.83 -1.34 0.08 6.04<br />
6046 RB10866 82 249 hypothetical protein 5899291 5899043 10.2 9214 - 6.26 -1.25 0.17 13.45<br />
6225 RB11190 90 273 hypothetical protein 6071212 6070940 12.2 10137 - 6.34 -1.33 0.14 10.26<br />
6258 RB11245 53 162 hypothetical protein 6099126 6098965 6.3 6073 - 6.39 -1.59 0.19 12.18<br />
6343 RB11392 148 447 conserved hypothetical protein 6177208 6177654 5 16382 + 6.42 -1.54 0.08 5.35<br />
6357 RB11416 363 1092 conserved hypothetical protein 6190901 6191992 5.2 41708 + 6.16 -1.02 0.05 4.68<br />
6371 RB11439 93 282 conserved hypothetical protein 6197993 6198274 10.6 10906 + 8.52 -1.91 0.07 3.90<br />
6385 RB11450 60 183 conserved hypothetical protein 6200046 6200228 11.8 6517 + 9.19 -1.82 0.19 10.33<br />
6557 RB11748 57 174 conserved hypothetical protein 6327844 6328017 5.8 6288 + 6.37 -1.55 0.22 14.12<br />
6569 RB11766 129 390 conserved hypothetical protein 6334355 6334744 10.4 14762 + 6.36 -1.62 0.20 12.35<br />
6579 RB11804 132 399 conserved hypothetical protein 6363537 6363139 11.3 15013 - 6.59 -1.97 0.07 3.63<br />
6580 RB11805 61 186 conserved hypothetical protein 6363715 6363530 12.1 7084 - 6.42 -1.78 0.17 9.76<br />
6635 RB11914 456 1371 narrowly conserved hypothetical protein with duf54 6411706 6413076 10 51304 + 6.27 -1.40 0.24 17.30<br />
6683 RB11992 205 618 conserved hypothetical protein, membrane 6469784 6470401 5.8 22120 + 6.24 -1.10 0.15 13.78<br />
6781 RB12193 36 111 conserved hypothetical protein 6575977 6575867 7.5 3719 - 6.33 -1.55 0.30 19.32<br />
6787 RB12201 69 210 conserved hypothetical protein 6578161 6577952 12.5 7444 - 7.35 -1.79 0.06 3.35<br />
6801 RB12220 171 516 conserved hypothetical protein 6587027 6586512 4.7 19504 - 6.25 -1.16 0.07 5.89<br />
6814 RB12241 417 1254 protein containing pectin lyase fold, secreted 6596139 6594886 6.2 45655 - 6.24 -1.17 0.15 12.80<br />
6818 RB12250 104 315 conserved hypothetical protein 6597248 6597562 11.9 12364 + 6.29 -1.17 0.19 16.31<br />
6822 RB12260 81 246 hypothetical protein-signal peptide prediction 6600777 6600532 11.9 9294 - 6.18 -1.15 0.21 18.59<br />
6857 RB12318 55 168 conserved hypothetical protein 6625565 6625398 11 6095 - 6.33 -1.65 0.33 20.31<br />
6936 RB12447 103 312 conserved hypothetical protein 6700272 6700583 10.6 11445 + 6.52 -2.00 0.21 10.45<br />
6941 RB12454 199 600 conserved hypothetical protein 6703647 6703048 10.3 22347 - 6.31 -1.44 0.30 20.67<br />
6996 RB12557 41 126 conserved hypothetical protein 6765784 6765659 9.2 4684 - 6.33 -1.55 0.06 3.97<br />
7009 RB12581 174 525 conserved hypothetical protein 6780597 6780073 9.9 19433 - 6.21 -1.25 0.27 21.95<br />
7023 RB12610 85 258 conserved hypothetical protein 6793700 6793957 9.7 9221 + 7.22 -1.28 0.28 22.15<br />
7141 RB12818 163 492 conserved hypothetical protein 6915989 6915498 11.7 18010 - 6.57 -2.38 0.11 4.47<br />
60min ID Locus AA Nuc Product Gene EC Start Stop IEP MW(kDa) Strand Intensity Ratio Stdev %<br />
3572 RB6353 205 618 cytochrome c oxidase, cbb3-type, subunit III 1.9.3.1 3336344 3336961 4.3 22849 + 6.01 -1.08 0.17 15.45<br />
5350 RB9585 328 987 Luciferase-like monooxygenase 5152143 5153129 6.3 36719 + 6.23 -1.00 0.05 4.75<br />
5698 RB10216 296 891 H+-transporting two-sector ATPase, gamma subunit 5518520 5519410 6 32680 + 6.09 -1.00 0.02 2.40<br />
5699 RB10217 479 1440 ATP synthase beta subunit 5519560 5520999 4.9 52151 + 6.33 -1.78 0.11 5.93<br />
5747 RB10310 485 1458 NADH dehydrogenase I chain M 5572980 5571523 6.5 52810 - 6.00 -1.00 0.14 14.34<br />
6842 RB12291 99 300 NAD(P) transhydrogenase subunit alpha 1.6.1.2 6612871 6612572 5.4 10186 - 6.13 -1.00 0.19 19.18<br />
827 RB1538 489 1470 Diaminopimelate decarboxylase lysA 4.1.1.20 790083 788614 6.4 55194 - 6.29 -1.02 0.13 13.19<br />
1967 RB3603 344 1035 secreted protein 1875461 1874427 4.6 37999 - 6.21 -1.38 0.17 12.04<br />
4275 RB7585 481 1446 probable glycine dehydrogenase [decarboxylating] subunit 1 1.4.4.2 4078350 4076905 5.2 52250 - 6.30 -1.07 0.19 17.99<br />
5850 RB10512 903 2712 conserved hypothetical protein, membrane 5704376 5701665 5.9 99270 - 6.26 -1.35 0.33 24.63<br />
6865 RB12327 686 2061 TGF-beta receptor, type I/II extracellular region 6633918 6631858 4.5 75645 - 6.68 -2.84 0.21 7.43<br />
7195 RB12921 683 2052 transketolase 1 TKL1 2.2.1.1 6957196 6959247 4.9 73380 + 6.34 -1.18 0.26 21.86<br />
3336 RB5918 252 759 biotin-protein ligase 3098715 3097957 5.2 26935 - 6.11 -1.19 0.29 24.55<br />
5925 RB10654 179 540 Phosphopantetheine adenylyltransferase coaD 2.7.7.3 5782824 5783363 5.9 19442 + 6.25 -1.18 0.07 5.82<br />
6040 RB10855 395 1188 mandelate racemase/muconate lactonizing enzyme family protein 5895378 5894191 6.4 43363 - 6.39 -1.27 0.01 0.68<br />
179 RB314 309 930 malonyl CoA-acyl carrier protein transacylase fabD 2.3.1.39 156968 157897 4.4 32349 + 7.49 -2.09 0.25 12.07<br />
5375 RB9629 330 993 acyl-lipid delta-9 desaturase ADS2 1.14.19.- 5184385 5183393 6.5 37640 - 5.86 -1.19 0.16 13.88<br />
4413 RB7818 121 366 Ribosomal protein S12/S23 4185730 4186095 11.8 13642 + 7.92 -1.21 0.03 2.85<br />
4423 RB7829 108 327 Ribosomal protein S10 4192031 4192357 10.3 11931 + 7.54 -2.06 0.29 13.96<br />
4426 RB7834 222 669 Ribosomal protein L4/L1e 4193390 4194058 10.7 24288 + 7.20 -1.52 0.13 8.43<br />
4442 RB7859 177 534 Ribosomal protein S5 4201351 4201884 10.6 18782 + 6.45 -1.52 0.11 7.35<br />
4750 RB8459 55 168 30S ribosomal protein S18 rpsR 4518496 4518663 10.5 6203 + 7.41 -1.90 0.09 4.61<br />
4824 RB8580 113 342 sigma-54 modulation protein 4577393 4577734 5.7 12706 + 7.01 -1.48 0.13 8.87<br />
5832 RB10471 151 456 Ribosomal protein L13 5678759 5678304 10 17369 - 7.58 -1.94 0.27 14.04<br />
7145 RB12824 146 441 Ribosomal protein S16 6917743 6917303 5.3 15885 - 7.05 -1.65 0.22 13.23<br />
2610 RB4681 65 198 cold shock protein cspA 2391934 2392131 4.7 6982 + 7.60 -2.42 0.13 5.18<br />
3525 RB6271 325 978 Tyrosine recombinase xerD xerD 3294814 3295791 10 36623 + 6.41 -1.51 0.18 11.94<br />
3528 RB6276 105 318 Histone-like bacterial DNA-binding protein 3297504 3297187 10.4 11299 - 7.89 -2.79 0.46 16.58<br />
3663 RB6492 199 600 recombination protein RecR recR 3439295 3438696 5.5 21844 - 6.50 -1.05 0.26 24.79<br />
3524 RB6269 405 1218 UDP-glucose 4-epimerase galE 5.1.3.2 3294798 3293581 5.3 44117 - 6.17 -1.14 0.23 20.17<br />
5168 RB9245 356 1071 lipopolysaccharide heptosyltransferase-1 2.-.-.- 4945117 4944047 6.5 39491 - 6.77 -1.61 0.06 3.82<br />
1948 RB3571 1181 3546 hypothetical protein 1858557 1855012 5.8 131252 - 6.05 -1.14 0.08 7.37<br />
6468 RB11597 387 1164 Type 4 fimbrial assembly protein pilC pilC 6254216 6255379 10.3 42633 + 6.23 -1.11 0.09 8.38<br />
6968 RB12502 435 1308 flagellar motor switch protein FliG fliG 6732844 6731537 7 47011 - 6.36 -1.04 0.11 11.02<br />
3242 RB5753 160 483 bacterioferritin comigratory protein 3004163 3004645 7.5 17860 + 6.24 -1.04 0.22 21.03<br />
374 RB684 653 1962 Arylsulfatase precursor atsA 3.1.6.1 368557 366596 5.5 73598 - 6.24 -1.06 0.23 21.69<br />
2363 RB4273 518 1557 iron-dependent peroxidase 1.1.1.- 2193431 2191875 5.1 57672 - 5.67 -1.25 0.24 19.02<br />
7313 RB13148 1012 3039 arylsulfatase A [precursor] 7058006 7054968 6.2 111097 - 6.45 -1.39 0.30 21.91<br />
1332 RB2479 273 822 conserved hypothetical protein 1296683 1295862 5.5 30436 - 6.05 -1.21 0.11 9.41<br />
4255 RB7557 327 984 von Willebrand factor type A domain protein 4058936 4059919 4.9 35328 + 5.89 -1.04 0.15 14.33<br />
5016 RB8937 560 1683 NADH oxidase 1.6.99.3 4763877 4762195 5.3 59977 - 6.30 -1.37 0.33 24.39<br />
5275 RB9445 249 750 integral membrane protein 5074872 5075621 10.4 26862 + 6.00 -1.04 0.19 17.86<br />
6639 RB11920 249 750 Protein of unknown function Zn-binding 6418443 6417694 9.1 27107 - 6.61 -1.20 0.13 10.91<br />
6983 RB12532 405 1218 metallophosphoesterase, secreted 6749031 6750248 5.8 45205 + 6.43 -1.28 0.17 13.49<br />
594 RB1129 895 2688 conserved hypothetical protein 583271 580584 5.8 99242 - 6.67 -1.43 0.15 10.38
5740 RB10297 196 591 formaldehyde activating enzyme fae 5563081 5562491 5.5 20967 - 6.44 -1.24 0.28 22.53<br />
4269 RB7578 189 570 Carbon storage regulator 4070466 4071035 8.8 20421 + 7.06 -1.94 0.30 15.58<br />
4477 RB7919 793 2382 serine/threonine protein kinase 4231818 4229437 5.4 87667 - 6.23 -1.44 0.22 15.42<br />
5722 RB10261 81 246 Carbon storage regulator csrA 5545964 5545719 5.1 8704 - 7.78 -1.69 0.42 24.78<br />
6071 RB10907 252 759 conserved hypothetical protein 5920466 5919708 4.5 28547 - 6.21 -1.00 0.16 16.07<br />
6223 RB11185 303 912 universal stress protein family 6069342 6068431 5.4 33738 - 6.39 -1.57 0.17 10.69<br />
2 RB2 59 180 secreted protein 182 3 3.7 6489 - 7.28 -3.23 0.43 13.21<br />
56 RB95 157 474 conserved hypothetical protein, membrane 40090 39617 9.6 17913 - 6.13 -1.06 0.14 12.87<br />
70 RB125 297 894 membrane protein 54571 53678 6 32981 - 6.25 -1.15 0.02 1.55<br />
182 RB319 95 288 hypothetical protein 159334 159621 10.2 10505 + 6.49 -1.37 0.23 16.42<br />
250 RB434 65 198 hypothetical protein 231791 231988 7.4 7385 + 6.40 -1.08 0.23 21.37<br />
472 RB869 71 216 hypothetical protein 460848 461063 4.4 8070 + 6.27 -1.60 0.37 23.29<br />
553 RB1036 480 1443 protein conserved in bacteria 539939 541381 5.8 51719 + 6.23 -1.39 0.30 21.50<br />
636 RB1199 181 546 hypothetical protein 611479 612024 5.7 19929 + 6.30 -1.19 0.20 17.02<br />
852 RB1588 151 456 ACP-like protein 817362 817817 5 17451 + 6.57 -1.14 0.22 19.59<br />
883 RB1644 123 372 secreted protein 845824 845453 9.7 13720 - 8.37 -2.54 0.17 6.67<br />
980 RB1860 110 333 hypothetical protein 960361 960693 10.9 11827 + 6.28 -1.37 0.24 17.17<br />
1130 RB2150 93 282 hypothetical protein 1138311 1138030 4.4 10755 - 7.50 -2.08 0.26 12.51<br />
1183 RB2247 579 1740 conserved hypothetical protein containing YTV domains 1181238 1179499 9.8 65013 - 7.62 -1.37 0.13 9.50<br />
1472 RB2714 302 909 protein containing DUF541 1415817 1414909 9.5 33087 - 6.35 -1.17 0.12 10.16<br />
1660 RB3057 72 219 hypothetical protein 1579920 1580138 10.8 8044 + 6.25 -1.07 0.20 18.82<br />
1676 RB3097 117 354 hypothetical protein 1606459 1606106 11.3 12852 - 7.20 -1.09 0.02 1.99<br />
1701 RB3147 168 507 membrane protein 1624840 1624334 8.7 18759 - 6.07 -1.02 0.07 7.20<br />
1825 RB3362 87 264 hypothetical protein 1728546 1728283 11.9 10017 - 6.14 -1.08 0.18 16.46<br />
1827 RB3365 43 132 hypothetical protein 1730124 1730255 11.4 4886 + 8.51 -2.76 0.21 7.65<br />
1844 RB3394 36 111 hypothetical protein 1752044 1752154 10.5 4239 + 5.92 -1.10 0.22 19.88<br />
1845 RB3395 398 1197 secreted protein containing DUF1559 1752151 1753347 7.8 43136 + 6.19 -1.25 0.21 16.57<br />
1980 RB3623 60 183 hypothetical protein 1886670 1886488 6.1 6661 - 5.83 -1.12 0.18 16.07<br />
2200 RB3981 161 486 hypothetical protein 2058000 2058485 4.1 18283 + 6.14 -1.02 0.19 18.25<br />
2312 RB4194 53 162 hypothetical protein 2156685 2156524 11.4 5728 - 7.47 -1.86 0.40 21.41<br />
2350 RB4255 204 615 hypothetical protein 2180806 2180192 10.3 21582 - 9.17 -1.16 0.16 14.06<br />
2354 RB4260 262 789 membrane protein 2181703 2182491 10 27432 + 6.24 -1.06 0.19 17.67<br />
2369 RB4283 499 1500 PvdD/PvdJ 2201462 2199963 5.7 56411 - 6.09 -1.30 0.19 14.97<br />
2528 RB4547 422 1269 hypothetical protein 2330116 2328848 7.6 46274 - 5.66 -1.05 0.14 13.38<br />
2553 RB4590 74 225 hypothetical protein 2352024 2351800 10.1 8330 - 9.62 -3.10 0.58 18.73<br />
2593 RB4652 52 159 hypothetical protein 2380494 2380652 12.6 5628 + 7.17 -1.16 0.09 7.69<br />
2596 RB4657 123 372 hypothetical protein 2381865 2382236 12.2 13398 + 6.34 -1.64 0.08 5.14<br />
2746 RB4897 184 555 hypothetical protein 2519505 2518951 10.9 20446 - 5.83 -1.12 0.16 14.49<br />
2789 RB4966 84 255 hypothetical protein 2552311 2552057 11.3 10016 - 9.22 -2.29 0.41 17.73<br />
2964 RB5262 95 288 membrane protein 2713742 2714029 6.3 10993 + 6.09 -1.18 0.27 23.23<br />
3239 RB5745 130 393 hypothetical protein 3001234 3000842 10.7 13522 - 7.22 -3.11 0.28 8.90<br />
3258 RB5779 187 564 hypothetical protein 3028410 3028973 4.4 21341 + 7.41 -1.29 0.12 9.08<br />
3271 RB5802 156 471 hypothetical protein 3039864 3040334 10.1 17001 + 6.68 -1.07 0.26 24.26<br />
3356 RB5957 154 465 secreted protein 3122093 3122557 3.3 15514 + 7.79 -1.21 0.28 23.57<br />
3430 RB6092 361 1086 Peptidase M50 3.4.24.- 3192387 3193472 9.6 39370 + 6.06 -1.09 0.13 11.78<br />
3466 RB6158 142 429 hypothetical protein 3233532 3233104 6 15746 - 6.27 -1.19 0.04 3.44<br />
3476 RB6174 69 210 hypothetical protein 3239734 3239525 10.6 7870 - 6.24 -1.16 0.03 2.25<br />
3725 RB6594 295 888 hypothetical protein 3505472 3506359 10.2 32951 + 6.23 -1.06 0.20 19.21<br />
3780 RB6699 47 144 hypothetical protein 3559781 3559924 11.1 5290 + 5.94 -1.03 0.15 14.12<br />
3802 RB6734 51 156 hypothetical protein 3577353 3577198 9 5639 - 6.35 -1.60 0.25 15.50<br />
3827 RB6778 59 180 hypothetical protein 3603142 3603321 5.5 6533 + 6.60 -1.00 0.21 21.13<br />
3836 RB6789 180 543 secreted protein 3608487 3607945 5 20191 - 6.53 -1.67 0.16 9.38<br />
3921 RB6951 1120 3363 hypothetical protein 3693377 3690015 4.6 123187 - 6.38 -1.11 0.21 18.97<br />
3971 RB7042 91 276 hypothetical protein 3743971 3744246 10.4 10835 + 6.23 -1.19 0.22 18.53<br />
4008 RB7108 91 276 hypothetical protein 3775303 3775578 10.9 10200 + 6.40 -1.39 0.32 22.89<br />
4016 RB7117 181 546 Ribosomal protein L35 3780322 3779777 11.4 19816 - 7.18 -1.88 0.04 2.26<br />
4049 RB7178 45 138 hypothetical protein 3806924 3807061 12.3 5320 + 7.21 -1.78 0.44 24.62<br />
4050 RB7180 104 315 hypothetical protein 3807345 3807031 10.8 11535 - 7.34 -1.58 0.35 22.40<br />
4175 RB7414 108 327 hypothetical protein 3965263 3964937 12.2 11737 - 6.41 -1.90 0.35 18.71<br />
4315 RB7647 73 222 hypothetical protein 4111931 4112152 7.4 8269 + 9.15 -2.21 0.18 8.35<br />
4377 RB7748 74 225 hypothetical protein 4150491 4150715 8.9 8433 + 6.44 -1.25 0.07 5.33<br />
4424 RB7832 50 153 hypothetical protein 4192499 4192651 13 5566 + 7.75 -1.99 0.41 20.42<br />
4568 RB8098 71 216 hypothetical protein 4337334 4337119 10.8 7950 - 9.48 -2.79 0.29 10.41<br />
4589 RB8144 143 432 hypothetical protein 4354622 4354191 10.1 16269 - 8.80 -1.44 0.17 11.63<br />
4669 RB8301 342 1029 membrane protein 4436412 4435384 9.7 37525 - 5.72 -1.13 0.23 20.52<br />
4759 RB8474 180 543 hypothetical protein 4524096 4523554 9.3 20951 - 6.24 -1.21 0.21 17.06<br />
4785 RB8518 370 1113 hypothetical protein 4543984 4545096 11.5 41176 + 6.24 -1.06 0.04 3.52<br />
4830 RB8594 41 126 hypothetical protein 4581645 4581770 9.2 4509 + 6.17 -1.40 0.11 7.51<br />
4861 RB8669 37 114 hypothetical protein 4614557 4614670 11.5 4395 + 8.68 -3.89 0.39 10.15<br />
4920 RB8776 86 261 hypothetical protein 4665512 4665772 9.3 9344 + 7.06 -1.50 0.34 22.51<br />
4952 RB8833 317 954 Zinc transporter ZIP 4696475 4697428 6.8 33766 + 6.39 -1.88 0.38 20.33<br />
5044 RB9009 257 774 secreted protein 4799393 4800166 6 29870 + 6.33 -1.33 0.15 11.03<br />
5068 RB9057 95 288 hypothetical protein 4834849 4834562 10.9 10885 - 7.36 -1.46 0.13 9.20<br />
5114 RB9142 131 396 hypothetical protein 4892884 4893279 11.3 14259 + 6.78 -1.48 0.34 23.07<br />
5117 RB9146 552 1659 conserved hypothetical protein, secreted 4895339 4893681 5.8 60781 - 6.11 -1.15 0.05 3.94<br />
5156 RB9228 637 1914 hypothetical protein 4934679 4936592 7.8 73174 + 6.34 -1.00 0.11 10.84<br />
5205 RB9324 267 804 secreted protein 4992474 4993277 6.7 29463 + 6.25 -1.94 0.32 16.21<br />
5225 RB9354 183 552 hypothetical protein 5011855 5012406 10.5 20002 + 6.42 -1.03 0.23 22.22<br />
5226 RB9356 129 390 protein containing DUF1579 5012771 5012382 9.2 14158 - 7.08 -1.73 0.26 15.03<br />
5236 RB9376 1827 5484 protein containing cadherin domain 5029102 5023619 3.8 197136 - 6.18 -1.03 0.18 17.39<br />
5280 RB9460 79 240 hypothetical protein 5078860 5078621 10.3 8991 - 8.38 -3.67 0.28 7.61<br />
5313 RB9520 532 1599 conserved hypothetical protein 5114495 5112897 4.4 59231 - 6.14 -1.08 0.04 4.10<br />
5446 RB9768 52 159 hypothetical protein 5264970 5264812 5.6 6062 - 6.12 -1.20 0.19 16.06<br />
5503 RB9872 67 204 hypothetical protein 5316692 5316895 5.4 6912 + 6.53 -1.96 0.22 11.15<br />
5574 RB10005 77 234 hypothetical protein 5389186 5389419 4.4 8625 + 6.38 -1.18 0.10 8.63<br />
5579 RB10014 534 1605 protein containing DUF1552 5395577 5393973 7.2 58534 - 6.25 -1.06 0.20 18.50<br />
5855 RB10521 53 162 hypothetical protein 5708182 5708343 9.1 6074 + 6.76 -1.45 0.16 10.73<br />
5885 RB10579 101 306 secreted protein 5739254 5738949 12.4 11507 - 7.41 -1.36 0.25 18.21<br />
5887 RB10581 384 1155 secreted protein containing DUF1559 5739353 5740507 6.2 41431 + 7.35 -1.29 0.23 17.63<br />
6042 RB10860 96 291 hypothetical protein 5896828 5896538 10 10535 - 6.23 -1.08 0.12 10.93<br />
6043 RB10861 46 141 hypothetical protein 5896851 5896991 4.2 4833 + 7.18 -1.15 0.10 8.56<br />
6045 RB10864 314 945 hypothetical protein 5898139 5899083 4.7 34608 + 6.34 -1.11 0.13 11.66<br />
6232 RB11204 170 513 hypothetical protein 6077364 6076852 10.6 19111 - 6.50 -1.57 0.35 22.59<br />
6258 RB11245 53 162 hypothetical protein 6099126 6098965 6.3 6073 - 6.41 -1.29 0.28 21.59<br />
6280 RB11287 75 228 hypothetical protein 6118405 6118632 9.1 8478 + 6.21 -1.10 0.26 23.51<br />
6304 RB11319 551 1656 hypothetical protein 6136312 6134657 6.1 63215 - 6.00 -1.09 0.03 2.33<br />
6336 RB11383 573 1722 conserved hypothetical protein 6174244 6175965 4.5 66716 + 6.42 -1.05 0.03 2.39<br />
6343 RB11392 148 447 conserved hypothetical protein 6177208 6177654 5 16382 + 6.51 -1.50 0.21 14.30<br />
6371 RB11439 93 282 conserved hypothetical protein 6197993 6198274 10.6 10906 + 8.85 -1.73 0.06 3.47<br />
6385 RB11450 60 183 conserved hypothetical protein 6200046 6200228 11.8 6517 + 9.28 -1.87 0.24 12.91<br />
6536 RB11703 52 159 conserved hypothetical protein 6311093 6311251 12 6046 + 6.38 -1.38 0.15 10.98<br />
6569 RB11766 129 390 conserved hypothetical protein 6334355 6334744 10.4 14762 + 6.41 -1.77 0.23 12.92<br />
6579 RB11804 132 399 conserved hypothetical protein 6363537 6363139 11.3 15013 - 6.63 -2.17 0.34 15.73<br />
6580 RB11805 61 186 conserved hypothetical protein 6363715 6363530 12.1 7084 - 6.63 -2.06 0.38 18.59<br />
6642 RB11925 76 231 conserved hypothetical protein 6419855 6420085 12.2 8248 + 6.07 -1.50 0.33 21.69<br />
6683 RB11992 205 618 conserved hypothetical protein, membrane 6469784 6470401 5.8 22120 + 6.41 -1.23 0.05 3.85<br />
6730 RB12084 285 858 conserved hypothetical protein, secreted 6520429 6519572 4.7 31843 - 6.44 -1.04 0.06 5.85<br />
6744 RB12114 110 333 conserved hypothetical protein 6540259 6539927 11.8 12716 - 6.29 -1.04 0.17 16.37<br />
6781 RB12193 36 111 conserved hypothetical protein 6575977 6575867 7.5 3719 - 6.57 -2.25 0.24 10.52<br />
6787 RB12201 69 210 conserved hypothetical protein 6578161 6577952 12.5 7444 - 7.70 -1.58 0.06 4.09<br />
6801 RB12220 171 516 conserved hypothetical protein 6587027 6586512 4.7 19504 - 6.39 -1.71 0.37 21.37<br />
6857 RB12318 55 168 conserved hypothetical protein 6625565 6625398 11 6095 - 6.43 -1.65 0.20 11.96<br />
6907 RB12403 59 180 conserved hypothetical protein 6673741 6673562 7.3 6301 - 6.48 -1.46 0.03 2.18<br />
6920 RB12426 137 414 conserved hypothetical protein, membrane 6684059 6684472 10.6 15166 + 6.05 -1.37 0.16 11.52<br />
6936 RB12447 103 312 conserved hypothetical protein 6700272 6700583 10.6 11445 + 6.59 -1.39 0.28 20.43<br />
6953 RB12477 354 1065 conserved hypothetical protein, secreted 6716008 6717072 7.6 39728 + 6.61 -2.10 0.03 1.45<br />
6992 RB12550 63 192 conserved hypothetical protein 6762725 6762534 11.9 7124 - 6.50 -2.30 0.18 7.72<br />
6996 RB12557 41 126 conserved hypothetical protein 6765784 6765659 9.2 4684 - 6.28 -1.33 0.28 21.31<br />
7009 RB12581 174 525 conserved hypothetical protein 6780597 6780073 9.9 19433 - 6.32 -1.71 0.11 6.46<br />
7365 RB13247 185 558 conserved hypothetical protein 7104177 7103620 10.2 21780 - 6.31 -1.40 0.23 16.26<br />
300min ID Locus AA Nuc Product Gene EC Start Stop IEP MW(kDa) Strand Intensity Ratio Stdev %<br />
5699 RB10217 479 1440 ATP synthase beta subunit 5519560 5520999 4.9 52151 + 5.86 -1.32 0.26 19.56
5700 RB10219 128 387 ATP synthase epsilon chain atpE 3.6.3.14 5521003 5521389 4.6 14002 + 5.48 -1.03 0.05 4.49<br />
515 RB950 442 1329 Cationic amino acid transporter 501765 500437 9.7 46180 - 5.94 -1.05 0.18 17.16<br />
1782 RB3287 527 1584 ABC-transporter, ATP-binding protein 1688084 1689667 6.8 57635 + 5.33 -1.20 0.06 5.12<br />
1967 RB3603 344 1035 secreted protein 1875461 1874427 4.6 37999 - 5.88 -1.85 0.11 6.00<br />
2109 RB3830 214 645 ARYLESTERASE PROTEIN 1987949 1988593 5.6 23495 + 6.00 -1.08 0.26 24.37<br />
2359 RB4269 282 849 glutamic acid specific endopeptidase, GSE=serine endopeptidase 2185049 2184201 5.6 31283 - 5.93 -1.85 0.15 8.27<br />
3301 RB5861 400 1203 FAD dependent oxidoreductase 1.4.3.3 3073915 3075117 5.9 44955 + 5.74 -1.02 0.14 13.81<br />
4534 RB8038 1381 4146 Hydantoinase/oxoprolinase 4298294 4302439 5.3 147404 + 5.83 -1.22 0.24 20.08<br />
4645 RB8254 729 2190 amino acid transporter, permease 4412322 4414511 10.1 77104 + 5.34 -1.19 0.10 8.28<br />
6865 RB12327 686 2061 TGF-beta receptor, type I/II extracellular region 6633918 6631858 4.5 75645 - 6.01 -1.81 0.30 16.29<br />
2192 RB3967 544 1635 sodium:proline/pantothenate symporter (proline/pantothenate permease) 2053237 2054871 9.4 59256 + 5.96 -1.57 0.23 14.67<br />
3721 RB6588 279 840 Purine phosphorylase, family 2 2.4.2.- 3502583 3501744 4.8 30449 - 5.52 -1.03 0.15 14.70<br />
3863 RB6844 316 951 Ribose-phosphate pyrophosphokinase prs 2.7.6.1 3637931 3638881 7 34778 + 6.09 -1.61 0.11 6.88<br />
571 RB1074 353 1062 Bacterial inner-membrane translocator 558194 559255 9.2 36366 + 5.74 -1.20 0.11 8.80<br />
1423 RB2627 342 1029 Glyceraldehyde 3-phosphate dehydrogenase gap 1.2.1.12 1364551 1365579 5.4 36716 + 6.60 -1.97 0.36 18.33<br />
1905 RB3496 511 1536 ribose ABC transporter, ATP-binding protein rbsA 3.6.3.17 1808754 1810289 5.4 56465 + 5.86 -1.35 0.15 10.94<br />
2999 RB5321 294 885 protein containing xylose isomerase-like TIM barrel 2748548 2749432 4.5 33540 + 5.59 -1.15 0.07 5.89<br />
3840 RB6793 350 1053 conserved hypothetical protein, membrane 3610014 3608962 9.5 37820 - 5.87 -1.06 0.23 21.51<br />
2295 RB4160 386 1161 conserved hypothetical protein 2136438 2137598 5.1 43127 + 6.24 -1.05 0.16 14.75<br />
179 RB314 309 930 malonyl CoA-acyl carrier protein transacylase fabD 2.3.1.39 156968 157897 4.4 32349 + 6.97 -2.82 0.29 10.16<br />
2733 RB4876 397 1194 delta 9 acyl-lipid fatty acid desaturase 1.14.19.- 2506214 2505021 9.5 44741 - 7.89 -1.93 0.11 5.49<br />
145 RB264 64 195 Ribosomal protein S21 132677 132871 12.3 8074 + 6.62 -1.35 0.31 23.23<br />
658 RB1233 206 621 30S ribosomal protein S4 rpsD 629490 628870 11.2 23193 - 6.85 -1.23 0.25 20.48<br />
2178 RB3942 177 534 SpoU rRNA methylase family protein 2.1.1.- 2039096 2038563 8.8 20222 - 5.88 -1.22 0.17 13.67<br />
3062 RB5434 695 2088 Elongation factor G 1 fusA1 2817863 2815776 4.7 77788 - 5.76 -1.01 0.24 24.09<br />
3272 RB5804 818 2457 polynucleotide phosphorylase 3040160 3042616 5.3 88202 + 6.04 -1.26 0.08 6.12<br />
3627 RB6436 320 963 Tryptophanyl-tRNA synthetase trpS 6.1.1.2 3389983 3389021 5.5 35532 - 5.83 -1.08 0.19 17.96<br />
3959 RB7022 90 273 Ribosomal protein S20 3735291 3735563 12.3 10255 + 6.70 -1.45 0.30 20.87<br />
4423 RB7829 108 327 Ribosomal protein S10 4192031 4192357 10.3 11931 + 7.75 -1.55 0.17 10.62<br />
4426 RB7834 222 669 Ribosomal protein L4/L1e 4193390 4194058 10.7 24288 + 6.99 -1.47 0.19 12.63<br />
4427 RB7836 107 324 Ribosomal L23 protein 4194060 4194383 10.8 12651 + 7.37 -1.94 0.18 9.20<br />
4428 RB7837 286 861 Ribosomal protein L2 4194481 4195341 11.8 31108 + 6.87 -1.30 0.20 15.22<br />
4429 RB7838 89 270 Ribosomal protein S19/S15 4195376 4195645 10.8 9943 + 6.78 -1.79 0.24 13.47<br />
4430 RB7839 119 360 Ribosomal protein L22/L17 4195706 4196065 11 13471 + 6.88 -1.44 0.25 17.51<br />
4432 RB7841 138 417 Ribosomal protein L16 4196810 4197226 11.1 15313 + 6.60 -1.78 0.40 22.19<br />
4434 RB7849 108 327 Ribosomal protein S17 4197700 4198026 10 12074 + 6.61 -2.08 0.49 23.57<br />
4435 RB7850 122 369 Ribosomal protein L14b/L23e 4198081 4198449 11 13526 + 6.64 -1.99 0.24 12.25<br />
4437 RB7852 196 591 50S ribosomal protein L5 rplE 4198935 4199525 10.4 22118 + 6.27 -1.50 0.22 14.36<br />
4438 RB7854 61 186 Ribosomal protein S14 4199624 4199809 11.8 6962 + 6.66 -2.04 0.24 11.84<br />
4441 RB7857 149 450 Ribosomal protein L18P/L5E 4200854 4201303 11.6 16541 + 6.30 -1.83 0.34 18.80<br />
4443 RB7863 164 495 Ribosomal protein L15 4202048 4202542 11.2 17961 + 5.78 -1.29 0.15 11.61<br />
4463 RB7899 141 426 50S ribosomal protein L11 rplK 4220226 4220651 9.6 15063 + 6.50 -1.56 0.31 19.55<br />
4824 RB8580 113 342 sigma-54 modulation protein 4577393 4577734 5.7 12706 + 7.16 -1.27 0.14 11.14<br />
5832 RB10471 151 456 Ribosomal protein L13 5678759 5678304 10 17369 - 7.34 -1.62 0.25 15.50<br />
7029 RB12623 127 384 Ribosomal protein S13 6796904 6797287 10.8 14481 + 6.99 -1.08 0.23 21.01<br />
7030 RB12625 127 384 Ribosomal protein S11 6797354 6797737 11.4 13570 + 7.05 -1.32 0.12 8.84<br />
7145 RB12824 146 441 Ribosomal protein S16 6917743 6917303 5.3 15885 - 6.78 -1.44 0.32 22.22<br />
7154 RB12839 225 678 Ribosomal protein L1 6921934 6922611 9.8 24159 + 7.19 -2.42 0.31 12.87<br />
4459 RB7894 398 1197 translation elongation factor EF-Tu 4217484 4218680 5.2 43184 + 7.36 -2.97 0.18 6.04<br />
2610 RB4681 65 198 cold shock protein cspA 2391934 2392131 4.7 6982 + 6.86 -2.85 0.20 7.15<br />
5823 RB10458 206 621 Rnpc2-prov protein 5671130 5670510 10.3 21043 - 6.46 -2.14 0.44 20.57<br />
6819 RB12251 567 1704 DNA-directed RNA polymerase specialized sigma factor 6599262 6597559 9.4 59396 - 5.88 -1.15 0.17 14.51<br />
7031 RB12626 331 996 DNA-directed RNA polymerase alpha chain rpoA 2.7.7.6 6797797 6798792 4.9 36760 + 6.44 -1.10 0.08 7.29<br />
2213 RB3999 436 1311 site-specific recombinase, 2064741 2063431 10.4 49552 - 7.16 -1.43 0.09 6.04<br />
3528 RB6276 105 318 Histone-like bacterial DNA-binding protein 3297504 3297187 10.4 11299 - 7.63 -2.57 0.17 6.46<br />
5531 RB9917 169 510 Single-strand binding protein/Primosomal replication protein n ssb 5344370 5343861 5.3 18108 - 6.78 -1.22 0.29 23.77<br />
1615 RB2978 152 459 nudix (nucleoside diphosphate linked moiety X)-type motif 2 1536161 1536619 5.9 17438 + 5.74 -1.06 0.18 17.01<br />
1334 RB2482 390 1173 glycosyl transferase, group 1 1298060 1296888 9.4 43071 - 5.70 -1.01 0.08 7.47<br />
1360 RB2512 325 978 GDP-mannose 4,6 dehydratase 1 GMD1 4.2.1.47 1314652 1313675 6.8 36583 - 5.91 -1.08 0.14 13.17<br />
2102 RB3817 470 1413 Membrane bound O-acyl transferase, MBOAT 1979134 1977722 10.4 53378 - 4.99 -1.05 0.19 18.32<br />
3524 RB6269 405 1218 UDP-glucose 4-epimerase galE 5.1.3.2 3294798 3293581 5.3 44117 - 5.86 -1.07 0.23 21.64<br />
3738 RB6624 503 1512 Bacterial sugar transferase 2.7.8.6 3520589 3522100 7.7 55315 + 6.07 -1.56 0.35 22.60<br />
3890 RB6895 401 1206 Glycosyl transferase, family 4 3666358 3665153 6.6 42272 - 6.06 -1.11 0.18 16.70<br />
5282 RB9463 628 1887 ATPase with chaperone activity 5079098 5080984 9.4 69732 + 6.05 -1.44 0.13 9.21<br />
236 RB406 557 1674 arylsulfatase 217496 215823 5.3 63383 - 5.67 -1.00 0.16 16.13<br />
642 RB1205 456 1371 choline sulfatase betC 3.1.6.6 615037 613667 6.6 51848 - 5.52 -1.19 0.16 13.06<br />
1850 RB3403 491 1476 arylsulfatase precursor arsA 3.1.6.8 1756892 1755417 5.5 55139 - 5.75 -1.18 0.28 23.88<br />
2363 RB4273 518 1557 iron-dependent peroxidase 1.1.1.- 2193431 2191875 5.1 57672 - 5.82 -1.10 0.13 11.39<br />
2899 RB5146 522 1569 arylsulfatase A precursor (ASA) ARSA 3.1.6.8 2645523 2647091 5 58243 + 5.72 -1.01 0.22 21.93<br />
7168 RB12861 309 930 Oligopeptide transport system permease protein OppB. oppB 6932929 6932000 9.5 33415 - 5.21 -1.33 0.11 8.15<br />
2075 RB3768 437 1314 Major facilitator superfamily MFS_1 1948093 1949406 8.6 47456 + 5.64 -1.10 0.16 14.83<br />
1644 RB3032 477 1434 Major facilitator superfamily MFS_1 1567813 1569246 9.8 52109 + 5.85 -1.38 0.29 21.10<br />
2729 RB4870 1081 3246 transporter, AcrB/D/F family 2502527 2499282 5 116198 - 5.85 -1.25 0.25 19.82<br />
4107 RB7276 916 2751 peptide synthetase 3866177 3868927 5 101234 + 5.80 -1.16 0.13 11.06<br />
5620 RB10081 680 2043 Lipid A export ATP-binding/permease protein msbA msbA 5441353 5439311 9.5 75625 - 5.70 -1.07 0.13 11.98<br />
1332 RB2479 273 822 conserved hypothetical protein 1296683 1295862 5.5 30436 - 6.11 -2.04 0.09 4.29<br />
342 RB609 737 2214 hypothetical protein 333445 331232 5.2 81456 - 5.75 -1.09 0.27 24.48<br />
1501 RB2771 479 1440 hypothetical protein 1443728 1442289 9.3 52483 - 5.91 -1.21 0.15 12.02<br />
2042 RB3710 524 1575 carbon-nitrogen hydrolase 1920808 1922382 5.3 60043 + 6.30 -1.24 0.20 16.54<br />
2076 RB3770 349 1050 NAD-dependent deyhydrogenase 1949403 1950452 4.9 39765 + 5.34 -1.00 0.17 16.91<br />
2774 RB4937 369 1110 oxidoreductase 2540086 2541195 5 39670 + 5.55 -1.14 0.10 8.73<br />
4255 RB7557 327 984 von Willebrand factor type A domain protein 4058936 4059919 4.9 35328 + 5.53 -1.20 0.16 13.11<br />
5275 RB9445 249 750 integral membrane protein 5074872 5075621 10.4 26862 + 5.75 -1.01 0.05 4.76<br />
5409 RB9692 159 480 protein containing DUF796 5222065 5222544 4.4 17120 + 5.41 -1.17 0.19 16.49<br />
6503 RB11650 459 1380 NADH-dependent dehydrogenase 6284983 6283604 5.8 50841 - 5.89 -1.02 0.08 7.40<br />
2057 RB3739 139 420 conserved hypothetical protein 1935471 1935052 4 15381 - 6.66 -1.22 0.30 24.26<br />
3274 RB5811 289 870 secreted hypothetical protein 3046272 3047141 4.9 32024 + 6.01 -1.02 0.23 22.73<br />
3329 RB5905 226 681 serine/threonine protein phosphatase 3094488 3095168 4.6 24910 + 6.05 -1.25 0.15 11.67<br />
5722 RB10261 81 246 Carbon storage regulator csrA 5545964 5545719 5.1 8704 - 7.81 -1.81 0.14 7.46<br />
6279 RB11286 131 396 TspO/MBR-related protein 6117961 6118356 9.8 15445 + 5.61 -1.00 0.14 13.81<br />
4825 RB8584 159 480 Nitrogen regulatory IIA protein ptsN 2.7.1.69 4577819 4578298 4.4 17423 + 6.78 -1.07 0.03 3.22<br />
2 RB2 59 180 secreted protein 182 3 3.7 6489 - 6.68 -3.57 0.53 14.95<br />
3 RB3 73 222 hypothetical protein 39 260 8.9 8439 + 6.08 -1.67 0.19 11.16<br />
43 RB73 98 297 membrane protein 28727 29023 10.1 11150 + 5.37 -1.14 0.15 12.98<br />
108 RB187 35 108 hypothetical protein 95474 95367 9.4 3992 - 5.71 -1.28 0.10 7.81<br />
114 RB197 53 162 hypothetical protein 102804 102965 9.1 6089 + 5.82 -1.22 0.12 10.03<br />
130 RB229 585 1758 secreted protein containing DUF1551 122782 121025 4.5 62454 - 5.82 -1.38 0.17 12.50<br />
133 RB241 47 144 hypothetical protein 124403 124546 11.4 5298 + 5.81 -1.29 0.04 3.13<br />
182 RB319 95 288 hypothetical protein 159334 159621 10.2 10505 + 6.30 -1.71 0.23 13.20<br />
193 RB337 97 294 hypothetical protein 169241 169534 12.5 11059 + 6.06 -1.65 0.37 22.49<br />
202 RB354 51 156 hypothetical protein 177535 177380 12 5703 - 5.87 -1.23 0.25 20.57<br />
209 RB367 67 204 hypothetical protein 183924 183721 10.4 7521 - 5.54 -1.38 0.02 1.77<br />
250 RB434 65 198 hypothetical protein 231791 231988 7.4 7385 + 6.14 -1.49 0.22 15.05<br />
279 RB475 727 2184 secreted protein 255858 253675 5.3 79993 - 5.44 -1.29 0.12 9.57<br />
330 RB573 42 129 hypothetical protein 313188 313316 11.3 4991 + 6.07 -1.63 0.19 11.76<br />
388 RB716 528 1587 protein containing DUF1501 384519 386105 7.3 58311 + 5.82 -1.09 0.17 15.22<br />
396 RB735 54 165 hypothetical protein 393774 393610 10.7 6032 - 6.41 -1.42 0.21 14.66<br />
409 RB767 311 936 conserved hypothetical protein, secreted 411420 412355 5.7 34002 + 5.80 -1.40 0.15 10.83<br />
413 RB776 62 189 hypothetical protein 416391 416203 9.6 7111 - 5.65 -1.00 0.13 12.64<br />
449 RB825 117 354 hypothetical protein 434184 433831 7.8 13302 - 5.92 -1.45 0.29 20.09<br />
466 RB861 499 1500 conserved hypothetical protein, membrane 457825 456326 5.6 54035 - 5.55 -1.01 0.23 23.15<br />
472 RB869 71 216 hypothetical protein 460848 461063 4.4 8070 + 6.11 -2.13 0.53 24.76<br />
473 RB870 428 1287 protein containing DUF1559 461164 462450 5.3 45928 + 6.64 -1.22 0.03 2.66<br />
503 RB930 41 126 hypothetical protein 492148 492023 10.2 4479 - 6.14 -1.19 0.25 20.97<br />
516 RB951 234 705 protein containing DUF1596 502459 501755 12.3 25583 - 6.35 -2.05 0.04 1.91<br />
622 RB1179 128 387 hypothetical protein 603998 604384 12 13863 + 7.20 -1.68 0.16 9.23<br />
699 RB1305 263 792 hypothetical protein 660019 660810 9.2 28661 + 5.96 -1.28 0.20 15.54<br />
781 RB1461 184 555 membrane protein 743517 742963 10 21051 - 5.24 -1.06 0.14 13.06<br />
849 RB1581 582 1749 membrane protein 814265 812517 6.7 65366 - 5.79 -1.33 0.14 10.16<br />
883 RB1644 123 372 secreted protein 845824 845453 9.7 13720 - 8.29 -1.52 0.20 13.37<br />
1012 RB1922 102 309 hypothetical protein 998917 998609 10.9 11658 - 6.02 -1.55 0.04 2.81<br />
1053 RB2011 93 282 hypothetical protein 1059411 1059692 4.4 9880 + 6.54 -1.71 0.20 11.81<br />
1088 RB2080 46 141 hypothetical protein 1091549 1091409 9.9 5071 - 6.48 -2.12 0.13 6.10
1099 RB2097 61 186 hypothetical protein 1106473 1106658 9.9 6506 + 6.00 -1.65 0.38 22.97<br />
1105 RB2105 470 1413 membrane protein 1114012 1112600 9.6 51853 - 6.19 -1.85 0.14 7.55<br />
1106 RB2107 93 282 hypothetical protein 1113972 1114253 10.1 10253 + 6.10 -1.45 0.32 22.32<br />
1130 RB2150 93 282 hypothetical protein 1138311 1138030 4.4 10755 - 7.13 -2.17 0.27 12.24<br />
1144 RB2175 98 297 membrane protein 1148511 1148807 10.3 10772 + 6.01 -1.07 0.10 9.69<br />
1183 RB2247 579 1740 conserved hypothetical protein containing YTV domains 1181238 1179499 9.8 65013 - 6.80 -2.30 0.26 11.45<br />
1184 RB2251 75 228 hypothetical protein 1181714 1181487 4.5 8027 - 7.17 -2.64 0.22 8.41<br />
1188 RB2255 628 1887 hypothetical protein 1186018 1184132 4.1 66330 - 6.50 -1.78 0.17 9.49<br />
1205 RB2281 310 933 hypothetical protein 1196032 1196964 11.2 33398 + 6.49 -1.21 0.07 5.75<br />
1217 RB2302 46 141 hypothetical protein 1204388 1204528 5.6 5335 + 6.73 -1.69 0.08 4.75<br />
1220 RB2306 41 126 hypothetical protein 1206782 1206907 9 4759 + 6.12 -1.51 0.26 17.12<br />
1236 RB2331 133 402 hypothetical protein 1215088 1214687 10.3 15081 - 6.01 -1.03 0.10 9.21<br />
1253 RB2353 124 375 membrane protein 1221696 1221322 10.4 13329 - 5.93 -1.18 0.25 21.13<br />
1265 RB2370 54 165 hypothetical protein 1229187 1229023 6.3 6204 - 5.63 -1.01 0.12 11.86<br />
1277 RB2384 65 198 hypothetical protein 1242697 1242500 12 7263 - 6.06 -1.73 0.36 20.91<br />
1285 RB2400 66 201 hypothetical protein 1248699 1248499 12.1 7112 - 5.71 -1.04 0.05 4.80<br />
1295 RB2420 547 1644 hypothetical protein 1258838 1260481 9.3 59939 + 5.75 -1.12 0.25 21.96<br />
1297 RB2425 102 309 hypothetical protein 1260766 1261074 11.1 11189 + 5.93 -1.44 0.30 20.99<br />
1310 RB2451 90 273 hypothetical protein 1278610 1278882 10.7 9921 + 5.89 -1.16 0.11 9.41<br />
1322 RB2467 66 201 hypothetical protein 1287454 1287654 11.3 7649 + 5.77 -1.00 0.05 4.52<br />
1338 RB2489 66 201 hypothetical protein 1301658 1301458 9 7521 - 5.09 -1.22 0.06 4.70<br />
1339 RB2490 43 132 hypothetical protein 1301758 1301627 7.4 4782 - 5.81 -1.03 0.20 19.30<br />
1352 RB2503 515 1548 O-antigen flippase wzx 1311056 1309509 10 55740 - 5.50 -1.19 0.22 18.78<br />
1424 RB2628 43 132 hypothetical protein 1365707 1365576 10 4777 - 5.98 -1.34 0.18 13.07<br />
1428 RB2636 116 351 hypothetical protein 1368450 1368100 11.7 12761 - 6.72 -1.94 0.09 4.59<br />
1446 RB2667 236 711 membrane protein 1387198 1386488 10.7 25698 - 5.73 -1.35 0.25 18.34<br />
1472 RB2714 302 909 protein containing DUF541 1415817 1414909 9.5 33087 - 5.90 -1.22 0.15 12.64<br />
1505 RB2782 589 1770 protein containing DUF1559 1445576 1447345 7.2 63826 + 7.37 -1.03 0.13 12.23<br />
1542 RB2852 240 723 conserved hypothetical protein, membrane 1482871 1482149 9.4 26727 - 5.59 -1.20 0.08 6.32<br />
1594 RB2948 148 447 membrane protein 1522349 1521903 9.5 16181 - 5.54 -1.00 0.14 13.81<br />
1597 RB2951 174 525 membrane protein 1523616 1523092 9.3 20061 - 5.57 -1.20 0.14 11.85<br />
1668 RB3067 153 462 membrane protein 1582850 1582389 7.6 17200 - 5.50 -1.00 0.05 5.03<br />
1672 RB3073 51 156 hypothetical protein 1585180 1585025 9.1 5384 - 5.80 -1.06 0.21 19.86<br />
1686 RB3119 195 588 membrane protein 1616510 1615923 9.3 22301 - 5.63 -1.09 0.08 7.38<br />
1688 RB3125 135 408 hypothetical protein 1618379 1617972 10.5 14861 - 6.90 -1.66 0.25 14.83<br />
1689 RB3127 68 207 hypothetical protein 1618378 1618584 12.3 7939 + 5.98 -1.14 0.21 18.58<br />
1692 RB3132 112 339 membrane protein 1619400 1619062 10.3 12831 - 5.72 -1.22 0.19 15.33<br />
1705 RB3152 68 207 hypothetical protein 1626667 1626461 12.2 7261 - 6.35 -1.44 0.25 17.46<br />
1767 RB3262 128 387 hypothetical protein 1678132 1678518 4.2 14073 + 5.93 -1.08 0.22 20.12<br />
1771 RB3268 231 696 hypothetical protein 1683003 1682308 12 25730 - 6.07 -1.60 0.26 16.36<br />
1776 RB3277 221 666 hypothetical protein 1686552 1685887 10.4 24222 - 6.12 -1.92 0.03 1.77<br />
1789 RB3301 50 153 hypothetical protein 1694208 1694056 10.5 5470 - 6.05 -1.19 0.06 5.39<br />
1827 RB3365 43 132 hypothetical protein 1730124 1730255 11.4 4886 + 8.08 -3.20 0.17 5.30<br />
1828 RB3366 78 237 hypothetical protein 1730380 1730616 5.6 9481 + 5.63 -1.02 0.23 23.12<br />
1833 RB3372 115 348 hypothetical protein 1733666 1734013 10 12916 + 5.56 -1.14 0.20 17.53<br />
1842 RB3391 79 240 hypothetical protein 1751875 1751636 12.4 9331 - 6.29 -1.47 0.21 14.23<br />
1843 RB3393 60 183 secreted protein 1751865 1752047 12.3 7156 + 6.21 -1.78 0.21 11.60<br />
1844 RB3394 36 111 hypothetical protein 1752044 1752154 10.5 4239 + 6.08 -1.87 0.32 17.39<br />
1845 RB3395 398 1197 secreted protein containing DUF1559 1752151 1753347 7.8 43136 + 6.09 -1.67 0.27 16.23<br />
1846 RB3398 67 204 hypothetical protein 1753293 1753496 11.4 7537 + 5.62 -1.04 0.17 16.08<br />
1847 RB3399 65 198 hypothetical protein 1753650 1753453 9.7 7418 - 6.36 -2.35 0.17 7.09<br />
1882 RB3455 57 174 hypothetical protein 1785458 1785631 10.2 6258 + 6.01 -1.08 0.26 24.06<br />
1892 RB3473 443 1332 membrane protein 1794413 1795744 4.8 47669 + 5.92 -1.32 0.23 17.48<br />
1904 RB3495 218 657 conserved hypothetical protein 1808101 1808757 5 23739 + 5.09 -1.03 0.16 16.01<br />
1942 RB3565 137 414 membrane protein 1853670 1854083 10.1 15653 + 5.53 -1.07 0.22 20.78<br />
1943 RB3566 64 195 hypothetical protein 1854080 1854274 9.4 7400 + 5.84 -1.32 0.13 9.67<br />
1946 RB3569 123 372 conserved hypothetical protein 1854728 1854357 7.5 13875 - 5.63 -1.04 0.18 17.73<br />
1950 RB3575 152 459 membrane protein 1858779 1859237 10.1 17195 + 5.72 -1.46 0.10 6.92<br />
1992 RB3644 127 384 hypothetical protein 1895184 1894801 4.9 14294 - 7.30 -1.99 0.26 12.98<br />
2025 RB3688 53 162 hypothetical protein 1910629 1910468 9.3 5981 - 6.07 -1.32 0.10 7.56<br />
2047 RB3718 535 1608 Major facilitator superfamily MFS_1 1927114 1925507 4.6 57372 - 5.63 -1.27 0.15 11.71<br />
2048 RB3719 141 426 hypothetical protein 1927500 1927075 10.8 16330 - 6.21 -2.10 0.25 12.06<br />
2113 RB3835 50 153 hypothetical protein 1989687 1989839 11.3 5789 + 5.88 -1.06 0.26 24.92<br />
2132 RB3867 284 855 Endonuclease/exonuclease/phosphatase 2000435 2001289 6.1 31682 + 6.13 -1.22 0.15 12.08<br />
2133 RB3868 125 378 hypothetical protein 2001663 2001286 11.9 14088 - 5.81 -1.03 0.13 12.99<br />
2139 RB3880 82 249 hypothetical protein 2006953 2006705 10.7 9535 - 6.05 -1.56 0.28 17.82<br />
2154 RB3905 38 117 hypothetical protein 2018601 2018717 5.1 4131 + 6.40 -1.54 0.14 8.81<br />
2166 RB3925 50 153 hypothetical protein 2033534 2033382 10.2 5399 - 5.89 -1.01 0.10 9.74<br />
2184 RB3953 857 2574 hypothetical protein 2048784 2046211 5.2 93881 - 5.87 -1.43 0.17 11.62<br />
2198 RB3975 204 615 hypothetical protein 2056726 2057340 12.3 22602 + 5.88 -1.04 0.02 2.36<br />
2200 RB3981 161 486 hypothetical protein 2058000 2058485 4.1 18283 + 5.83 -1.25 0.20 16.26<br />
2206 RB3990 55 168 hypothetical protein 2060721 2060888 11.2 6203 + 6.08 -1.56 0.12 7.83<br />
2210 RB3994 191 576 hypothetical protein 2061291 2061866 4.1 20739 + 5.86 -1.15 0.20 17.32<br />
2216 RB4002 483 1452 hypothetical protein 2065341 2066792 4.6 53759 + 5.75 -1.14 0.23 20.46<br />
2244 RB4061 488 1467 Peptidase A24A, prepilin type IV:Peptidase A24A, N-terminal 2095498 2096964 9.7 54251 + 5.76 -1.01 0.12 11.54<br />
2254 RB4084 119 360 hypothetical protein 2104677 2105036 10 13521 + 6.13 -1.61 0.13 7.83<br />
2258 RB4093 45 138 hypothetical protein 2107600 2107737 10 5285 + 6.10 -1.76 0.16 9.07<br />
2260 RB4097 733 2202 conserved hypothetical protein 2108244 2110445 6.2 83188 + 5.88 -1.23 0.21 16.86<br />
2280 RB4129 426 1281 conserved hypothetical protein 2125088 2126368 4.6 46461 + 5.93 -1.07 0.10 9.68<br />
2285 RB4142 43 132 hypothetical protein 2130009 2130140 12 4964 + 6.22 -1.43 0.31 21.46<br />
2287 RB4145 90 273 hypothetical protein 2131933 2131661 12 10727 - 6.35 -1.00 0.17 16.76<br />
2312 RB4194 53 162 hypothetical protein 2156685 2156524 11.4 5728 - 7.19 -1.98 0.19 9.44<br />
2343 RB4243 58 177 hypothetical protein 2174155 2174331 4.4 6442 + 5.72 -1.05 0.17 16.11<br />
2369 RB4283 499 1500 PvdD/PvdJ 2201462 2199963 5.7 56411 - 6.02 -1.75 0.06 3.71<br />
2371 RB4285 841 2526 membrane protein 2204136 2201611 5.9 93852 - 5.74 -1.02 0.06 5.75<br />
2416 RB4358 123 372 hypothetical protein 2239474 2239103 6.5 13459 - 6.13 -1.79 0.12 6.72<br />
2417 RB4360 156 471 hypothetical protein 2240075 2239605 4.7 17592 - 6.04 -1.43 0.02 1.23<br />
2424 RB4373 109 330 hypothetical protein 2242863 2242534 4.8 12263 - 6.15 -1.99 0.27 13.35<br />
2528 RB4547 422 1269 hypothetical protein 2330116 2328848 7.6 46274 - 5.57 -1.13 0.18 16.32<br />
2553 RB4590 74 225 hypothetical protein 2352024 2351800 10.1 8330 - 9.54 -2.32 0.27 11.42<br />
2593 RB4652 52 159 hypothetical protein 2380494 2380652 12.6 5628 + 6.73 -1.61 0.15 9.44<br />
2596 RB4657 123 372 hypothetical protein 2381865 2382236 12.2 13398 + 6.26 -1.93 0.16 8.08<br />
2631 RB4719 54 165 hypothetical protein 2419276 2419112 12.3 6311 - 5.48 -1.02 0.24 23.38<br />
2647 RB4740 50 153 hypothetical protein 2431814 2431966 11.4 5598 + 6.08 -1.87 0.30 15.91<br />
2664 RB4766 130 393 membrane protein 2442991 2442599 9.7 14452 - 5.33 -1.01 0.13 12.50<br />
2687 RB4801 96 291 hypothetical protein 2459502 2459212 9.8 10561 - 5.73 -1.07 0.13 12.03<br />
2698 RB4814 87 264 hypothetical protein 2467537 2467800 11.3 9301 + 6.40 -1.50 0.34 22.37<br />
2746 RB4897 184 555 hypothetical protein 2519505 2518951 10.9 20446 - 5.50 -1.11 0.16 14.10<br />
2777 RB4941 173 522 hypothetical protein 2541619 2542140 11.2 19306 + 5.74 -1.95 0.34 17.44<br />
2781 RB4951 95 288 hypothetical protein 2547498 2547785 12.1 10953 + 6.65 -1.71 0.33 19.12<br />
2789 RB4966 84 255 hypothetical protein 2552311 2552057 11.3 10016 - 9.52 -2.05 0.27 13.28<br />
2794 RB4972 56 171 hypothetical protein 2557220 2557390 9 6197 + 7.05 -1.33 0.19 14.53<br />
2825 RB5016 235 708 hypothetical protein 2579933 2579226 4.3 27492 - 5.13 -1.11 0.05 4.38<br />
2858 RB5076 113 342 hypothetical protein 2612505 2612164 10.5 12883 - 5.03 -1.13 0.24 21.16<br />
2936 RB5211 159 480 conserved hypothetical protein 2688867 2689346 7.4 17871 + 5.93 -1.15 0.12 10.39<br />
2964 RB5262 95 288 membrane protein 2713742 2714029 6.3 10993 + 5.76 -1.11 0.24 21.84<br />
3045 RB5409 97 294 hypothetical protein 2794195 2794488 12.7 10898 + 7.53 -3.71 0.16 4.40<br />
3049 RB5415 62 189 hypothetical protein 2798737 2798925 12.3 7098 + 6.53 -1.69 0.31 18.40<br />
3052 RB5418 73 222 secreted protein 2805185 2804964 11 8406 - 5.41 -1.10 0.24 21.92<br />
3239 RB5745 130 393 hypothetical protein 3001234 3000842 10.7 13522 - 6.75 -2.90 0.44 15.14<br />
3258 RB5779 187 564 hypothetical protein 3028410 3028973 4.4 21341 + 7.19 -1.11 0.18 15.93<br />
3271 RB5802 156 471 hypothetical protein 3039864 3040334 10.1 17001 + 6.76 -1.57 0.08 5.34<br />
3291 RB5841 670 2013 serine/threonine protein phosphatase family 3061924 3063936 5.4 73664 + 5.57 -1.05 0.09 8.83<br />
3356 RB5957 154 465 secreted protein 3122093 3122557 3.3 15514 + 7.13 -1.83 0.30 16.44<br />
3368 RB5976 54 165 hypothetical protein 3135318 3135482 11.8 6532 + 5.19 -1.04 0.19 17.81<br />
3388 RB6009 122 369 hypothetical protein 3149941 3149573 4.3 14124 - 5.86 -1.31 0.28 21.18<br />
3430 RB6092 361 1086 Peptidase M50 3.4.24.- 3192387 3193472 9.6 39370 + 5.85 -1.13 0.01 0.94<br />
3466 RB6158 142 429 hypothetical protein 3233532 3233104 6 15746 - 6.19 -1.66 0.30 17.97<br />
3476 RB6174 69 210 hypothetical protein 3239734 3239525 10.6 7870 - 5.70 -1.07 0.07 7.00<br />
3485 RB6193 62 189 hypothetical protein 3246096 3245908 11.4 6970 - 6.34 -2.04 0.21 10.19<br />
3513 RB6245 107 324 hypothetical protein 3278762 3279085 12.4 12397 + 6.25 -1.35 0.27 20.06<br />
3547 RB6314 52 159 hypothetical protein 3319320 3319478 6.3 6180 + 6.84 -3.31 0.23 6.95<br />
3559 RB6332 250 753 conserved hypothetical protein, secreted 3325222 3325974 9.7 27162 + 5.33 -1.07 0.09 8.81
3643 RB6462 90 273 hypothetical protein 3414908 3415180 9.9 10005 + 6.14 -1.08 0.01 0.52<br />
3644 RB6463 189 570 conserved hypothetical protein, membrane 3415155 3415724 10.2 19818 + 6.25 -1.10 0.14 12.86<br />
3652 RB6477 369 1110 hypothetical protein 3424543 3425652 5.7 39787 + 5.98 -1.48 0.14 9.42<br />
3666 RB6495 39 120 hypothetical protein 3441961 3441842 12.7 4765 - 5.88 -1.09 0.11 10.36<br />
3701 RB6553 74 225 hypothetical protein 3479627 3479403 12.1 8987 - 6.46 -1.94 0.05 2.74<br />
3709 RB6567 37 114 hypothetical protein 3489298 3489185 7.6 4468 - 5.99 -1.23 0.08 6.20<br />
3718 RB6583 996 2991 secreted protein 3498012 3501002 4.5 112232 + 6.01 -1.56 0.38 24.04<br />
3725 RB6594 295 888 hypothetical protein 3505472 3506359 10.2 32951 + 5.96 -1.67 0.22 12.98<br />
3731 RB6609 45 138 hypothetical protein 3511456 3511593 10.4 5004 + 6.73 -2.24 0.18 8.19<br />
3744 RB6634 365 1098 protein containing DUF1559 3525785 3526882 5.3 40217 + 6.03 -1.84 0.12 6.61<br />
3780 RB6699 47 144 hypothetical protein 3559781 3559924 11.1 5290 + 5.82 -1.08 0.22 20.49<br />
3820 RB6766 55 168 hypothetical protein 3597367 3597534 12 6133 + 6.04 -1.44 0.21 14.41<br />
3826 RB6777 137 414 hypothetical protein 3602786 3603199 11.5 15512 + 7.26 -1.59 0.13 8.21<br />
3827 RB6778 59 180 hypothetical protein 3603142 3603321 5.5 6533 + 6.65 -1.40 0.13 8.99<br />
3836 RB6789 180 543 secreted protein 3608487 3607945 5 20191 - 5.94 -1.48 0.03 2.36<br />
3867 RB6849 101 306 hypothetical protein 3642537 3642232 12.8 12002 - 6.06 -1.30 0.09 6.75<br />
3971 RB7042 91 276 hypothetical protein 3743971 3744246 10.4 10835 + 5.72 -1.22 0.13 10.76<br />
3977 RB7056 95 288 hypothetical protein 3746488 3746775 4.7 11164 + 5.91 -1.28 0.22 16.91<br />
4009 RB7109 53 162 hypothetical protein 3775707 3775546 6.7 5850 - 5.51 -1.06 0.14 12.86<br />
4015 RB7116 59 180 hypothetical protein 3779586 3779765 11.7 6458 + 6.93 -2.16 0.20 9.11<br />
4016 RB7117 181 546 Ribosomal protein L35 3780322 3779777 11.4 19816 - 6.82 -1.77 0.23 12.78<br />
4048 RB7177 35 108 hypothetical protein 3806891 3806784 12 4191 - 6.18 -1.18 0.02 1.55<br />
4049 RB7178 45 138 hypothetical protein 3806924 3807061 12.3 5320 + 6.74 -2.24 0.41 18.36<br />
4050 RB7180 104 315 hypothetical protein 3807345 3807031 10.8 11535 - 6.85 -2.34 0.21 9.15<br />
4051 RB7184 190 573 conserved hypothetical protein 3807517 3808089 9.2 21306 + 6.49 -1.92 0.26 13.78<br />
4092 RB7246 50 153 hypothetical protein 3850393 3850545 9.7 5973 + 6.80 -1.23 0.29 24.04<br />
4131 RB7313 51 156 hypothetical protein 3892097 3891942 10.5 5558 - 7.27 -3.17 0.11 3.44<br />
4163 RB7397 37 114 hypothetical protein 3956551 3956438 9.4 3746 - 5.60 -1.00 0.20 19.82<br />
4200 RB7455 838 2517 conserved hypothetical protein containing YTV domains 3999277 3996761 9 92217 - 6.71 -1.93 0.31 16.03<br />
4236 RB7525 281 846 secreted protein 4048930 4048085 4.2 27759 - 5.67 -2.67 0.02 0.59<br />
4237 RB7530 97 294 hypothetical protein 4049298 4049005 12.4 11656 - 6.16 -3.24 0.46 14.24<br />
4241 RB7535 136 411 hypothetical protein 4050024 4050434 10.9 15058 + 6.94 -1.20 0.11 9.39<br />
4251 RB7549 60 183 hypothetical protein 4057545 4057727 11.8 6684 + 5.61 -1.22 0.06 5.07<br />
4278 RB7589 86 261 hypothetical protein 4080238 4079978 12.1 9472 - 5.73 -1.26 0.04 3.50<br />
4314 RB7646 62 189 hypothetical protein 4111735 4111923 10.5 6950 + 6.71 -2.38 0.13 5.29<br />
4315 RB7647 73 222 hypothetical protein 4111931 4112152 7.4 8269 + 8.97 -2.89 0.15 5.18<br />
4327 RB7667 38 117 hypothetical protein 4117097 4116981 6.7 4315 - 7.56 -1.51 0.20 13.14<br />
4348 RB7709 134 405 protein containing DUF1582 4134747 4134343 11.9 15682 - 5.33 -1.02 0.23 22.89<br />
4384 RB7756 74 225 hypothetical protein 4154712 4154488 12.1 8781 - 6.28 -1.13 0.19 16.49<br />
4401 RB7791 533 1602 conserved hypothetical protein, membrane 4173444 4175045 6.3 57958 + 5.26 -1.04 0.03 2.98<br />
4422 RB7828 40 123 hypothetical protein 4192037 4191915 10.4 4526 - 7.62 -1.23 0.09 7.37<br />
4424 RB7832 50 153 hypothetical protein 4192499 4192651 13 5566 + 7.51 -1.85 0.23 12.27<br />
4481 RB7929 75 228 hypothetical protein 4236583 4236810 11.9 8270 + 5.56 -1.16 0.24 20.46<br />
4484 RB7937 41 126 hypothetical protein 4237809 4237684 10.7 4936 - 5.69 -1.08 0.13 11.67<br />
4486 RB7939 105 318 hypothetical protein 4238296 4238613 11 12019 + 6.22 -1.41 0.16 11.10<br />
4531 RB8034 147 444 membrane protein 4295344 4295787 10.2 16546 + 5.89 -1.37 0.11 8.26<br />
4552 RB8070 59 180 hypothetical protein 4319538 4319717 4.5 6478 + 5.78 -1.46 0.06 4.33<br />
4568 RB8098 71 216 hypothetical protein 4337334 4337119 10.8 7950 - 9.38 -2.59 0.23 8.96<br />
4571 RB8102 387 1164 protein containing DUF1559 4339048 4337885 4.5 42114 - 5.37 -1.27 0.18 13.82<br />
4578 RB8119 142 429 hypothetical protein 4346427 4346855 10.1 15659 + 5.67 -1.36 0.23 16.67<br />
4589 RB8144 143 432 hypothetical protein 4354622 4354191 10.1 16269 - 8.53 -1.02 0.16 15.55<br />
4613 RB8193 51 156 hypothetical protein 4382501 4382656 10.5 5994 + 5.88 -1.02 0.22 21.70<br />
4629 RB8227 59 180 hypothetical protein 4400365 4400186 9.3 6533 - 5.84 -1.03 0.14 13.11<br />
4677 RB8319 37 114 hypothetical protein 4442421 4442308 4.4 4328 - 5.74 -1.10 0.12 10.45<br />
4696 RB8353 82 249 hypothetical protein 4459374 4459622 10.8 9402 + 6.17 -3.27 0.44 13.45<br />
4718 RB8389 939 2820 membrane protein 4484734 4481915 10.1 105627 - 5.49 -1.11 0.11 9.87<br />
4749 RB8457 113 342 hypothetical protein 4518458 4518117 11.5 12525 - 7.08 -1.02 0.22 21.96<br />
4755 RB8468 88 267 hypothetical protein 4520799 4520533 11.9 9719 - 6.60 -1.20 0.15 12.67<br />
4830 RB8594 41 126 hypothetical protein 4581645 4581770 9.2 4509 + 6.06 -1.36 0.26 19.03<br />
4861 RB8669 37 114 hypothetical protein 4614557 4614670 11.5 4395 + 9.22 -2.87 0.27 9.24<br />
4971 RB8866 445 1338 secreted conserved hypothetical protein 4716964 4718301 6 49999 + 5.86 -1.27 0.05 4.08<br />
5052 RB9020 381 1146 secreted protein 4807486 4808631 5.9 42557 + 6.78 -1.76 0.42 23.89<br />
5066 RB9050 56 171 hypothetical protein 4829846 4829676 9 6404 - 5.81 -1.01 0.11 11.04<br />
5068 RB9057 95 288 hypothetical protein 4834849 4834562 10.9 10885 - 6.98 -1.70 0.20 11.66<br />
5074 RB9070 409 1230 secreted protein containing DUF1559 4840267 4841496 8.6 44198 + 5.38 -2.02 0.24 11.99<br />
5124 RB9160 118 357 hypothetical protein 4900491 4900847 10.8 12876 + 6.23 -1.47 0.00 0.34<br />
5204 RB9322 42 129 hypothetical protein 4992459 4992331 7.3 4319 - 5.71 -1.24 0.10 8.01<br />
5205 RB9324 267 804 secreted protein 4992474 4993277 6.7 29463 + 5.69 -1.32 0.18 13.55<br />
5214 RB9343 59 180 hypothetical protein 5003789 5003968 11.4 6521 + 6.01 -1.31 0.02 1.69<br />
5225 RB9354 183 552 hypothetical protein 5011855 5012406 10.5 20002 + 6.49 -1.04 0.15 14.62<br />
5258 RB9417 103 312 hypothetical protein 5050621 5050932 10.5 11390 + 5.90 -1.38 0.17 12.16<br />
5280 RB9460 79 240 hypothetical protein 5078860 5078621 10.3 8991 - 8.85 -2.89 0.23 8.11<br />
5313 RB9520 532 1599 conserved hypothetical protein 5114495 5112897 4.4 59231 - 5.87 -1.36 0.19 14.27<br />
5371 RB9624 357 1074 hypothetical protein 5181259 5180186 5.2 38849 - 5.76 -1.14 0.12 10.32<br />
5380 RB9641 73 222 hypothetical protein 5189877 5190098 12.1 7925 + 5.89 -1.04 0.20 19.50<br />
5419 RB9713 1082 3249 large, multifunctional secreted protein - Streptomyces coelicolor (fragment) 5243297 5240049 4.9 120210 - 5.83 -1.02 0.08 7.65<br />
5469 RB9806 165 498 membrane protein 5280590 5281087 10.2 18166 + 5.83 -1.04 0.07 6.44<br />
5488 RB9844 317 954 cytochrome c biogenesis protein, CcmF/CcyK/CcsA family 5301844 5302797 9.4 34187 + 6.03 -1.17 0.11 9.48<br />
5498 RB9861 98 297 hypothetical protein 5313829 5314125 10.7 10762 + 5.83 -1.13 0.15 13.00<br />
5503 RB9872 67 204 hypothetical protein 5316692 5316895 5.4 6912 + 5.99 -1.50 0.19 12.71<br />
5644 RB10123 72 219 hypothetical protein 5463443 5463661 10.8 8181 + 5.72 -1.19 0.14 11.46<br />
5662 RB10146 105 318 hypothetical protein 5476874 5476557 6.1 11800 - 5.33 -1.04 0.01 0.68<br />
5694 RB10209 110 333 H+-transporting two-sector ATPase, C subunit 3.6.3.14 5515135 5515467 10.2 11565 + 6.44 -1.58 0.28 17.80<br />
5837 RB10481 82 249 hypothetical protein 5685184 5685432 10.6 9492 + 5.77 -1.00 0.09 8.55<br />
5855 RB10521 53 162 hypothetical protein 5708182 5708343 9.1 6074 + 6.54 -1.30 0.27 20.40<br />
5885 RB10579 101 306 secreted protein 5739254 5738949 12.4 11507 - 7.16 -1.63 0.17 10.42<br />
5887 RB10581 384 1155 secreted protein containing DUF1559 5739353 5740507 6.2 41431 + 7.14 -1.58 0.21 13.04<br />
5908 RB10620 49 150 hypothetical protein 5768561 5768412 10.6 5707 - 6.80 -1.10 0.26 23.37<br />
5933 RB10668 58 177 hypothetical protein 5796528 5796352 9.3 6645 - 5.93 -1.17 0.20 16.79<br />
5937 RB10674 58 177 hypothetical protein 5800080 5799904 11.9 6706 - 5.59 -1.10 0.02 2.18<br />
5962 RB10712 84 255 hypothetical protein 5824976 5825230 10.2 9185 + 5.83 -1.34 0.28 21.12<br />
6048 RB10868 136 411 hypothetical protein 5900330 5899920 11.3 15100 - 6.78 -1.09 0.20 18.43<br />
6280 RB11287 75 228 hypothetical protein 6118405 6118632 9.1 8478 + 5.42 -1.10 0.27 24.70<br />
6343 RB11392 148 447 conserved hypothetical protein 6177208 6177654 5 16382 + 6.29 -1.37 0.05 3.61<br />
6364 RB11429 37 114 conserved hypothetical protein 6196478 6196591 12.4 4245 + 6.85 -2.38 0.14 5.98<br />
6367 RB11434 69 210 conserved hypothetical protein 6197442 6197233 9.8 7670 - 6.02 -1.16 0.20 16.89<br />
6371 RB11439 93 282 conserved hypothetical protein 6197993 6198274 10.6 10906 + 8.25 -3.09 0.16 5.22<br />
6385 RB11450 60 183 conserved hypothetical protein 6200046 6200228 11.8 6517 + 8.52 -2.44 0.17 6.89<br />
6539 RB11707 83 252 conserved hypothetical protein 6312937 6313188 9.8 9109 + 5.60 -1.34 0.19 14.46<br />
6569 RB11766 129 390 conserved hypothetical protein 6334355 6334744 10.4 14762 + 5.82 -1.20 0.26 21.35<br />
6579 RB11804 132 399 conserved hypothetical protein 6363537 6363139 11.3 15013 - 5.94 -1.05 0.18 17.53<br />
6623 RB11886 933 2802 protein containing DUF1585 6403682 6400881 5.1 105437 - 5.90 -1.00 0.19 18.57<br />
6626 RB11897 253 762 conserved hypothetical protein 6407046 6407807 11.3 27903 + 5.67 -1.01 0.02 1.89<br />
6697 RB12021 82 249 conserved hypothetical protein 6485018 6484770 11.9 9305 - 6.50 -1.93 0.23 11.74<br />
6744 RB12114 110 333 conserved hypothetical protein 6540259 6539927 11.8 12716 - 5.85 -1.09 0.04 3.38<br />
6781 RB12193 36 111 conserved hypothetical protein 6575977 6575867 7.5 3719 - 6.19 -1.89 0.19 10.31<br />
6787 RB12201 69 210 conserved hypothetical protein 6578161 6577952 12.5 7444 - 7.03 -1.25 0.20 16.25<br />
6822 RB12260 81 246 hypothetical protein-signal peptide prediction 6600777 6600532 11.9 9294 - 5.85 -1.18 0.11 9.43<br />
6858 RB12319 187 564 conserved hypothetical protein 6626077 6625514 11.2 21283 - 5.86 -1.16 0.16 13.98<br />
6867 RB12329 110 333 conserved hypothetical protein, membrane 6634234 6634566 4 11862 + 6.86 -2.13 0.23 10.84<br />
6904 RB12396 57 174 conserved hypothetical protein 6670870 6670697 11.3 6680 - 5.99 -1.54 0.22 14.34<br />
6920 RB12426 137 414 conserved hypothetical protein, membrane 6684059 6684472 10.6 15166 + 5.80 -1.06 0.09 8.88<br />
6936 RB12447 103 312 conserved hypothetical protein 6700272 6700583 10.6 11445 + 6.03 -1.21 0.23 18.72<br />
7009 RB12581 174 525 conserved hypothetical protein 6780597 6780073 9.9 19433 - 5.86 -1.27 0.17 13.18<br />
7141 RB12818 163 492 conserved hypothetical protein 6915989 6915498 11.7 18010 - 7.56 -3.62 0.19 5.24<br />
7153 RB12837 65 198 conserved hypothetical protein 6921732 6921929 9.8 7318 + 7.18 -1.45 0.16 10.88<br />
7323 RB13171 43 132 conserved hypothetical protein 7069906 7069775 4.4 4834 - 5.69 -1.02 0.16 16.06<br />
7342 RB13207 58 177 secreted protein 7086761 7086585 11.9 6443 - 6.10 -1.15 0.23 20.48<br />
7403 RB13327 421 1266 protein containing DUF1559 7143601 7142336 6.9 45140 - 5.17 -1.28 0.06 5.02
Cold Induced<br />
10min ID Locus AA Nuc Product Gene EC Start Stop IEP MW(kDa) Strand Intensity Stdev Ratio Stdev %<br />
5144 RB9200 283 852 4-nitrophenylphosphatase PHO13 3.1.3.41 4919220 4918369 4.9 30413 - 6.01 0.05 2.05 0.10 5.05<br />
212 RB370 553 1662 nitrate transporter substrate-binding protein 184428 186089 4.6 60742 + 6.07 0.04 1.50 0.19 12.45<br />
6089 RB10939 62 189 conserved hypothetical protein 5934628 5934816 4.3 6627 + 6.09 0.05 1.71 0.14 8.22<br />
2463 RB4433 162 489 Ferritin and Dps 2277208 2277696 4.3 17757 + 5.70 0.10 1.68 0.17 10.07<br />
5783 RB10378 144 435 Thioredoxin trxA 5610497 5610063 4.6 15274 - 6.37 0.07 1.96 0.10 5.05<br />
6216 RB11174 469 1410 metallo-beta-lactamase family protein 6065046 6063637 4.8 51160 - 6.00 0.16 1.80 0.14 7.52<br />
2488 RB4474 168 507 CsbD-like 2295048 2295554 8.8 18451 + 6.92 0.14 1.60 0.14 9.04<br />
6086 RB10934 68 207 CsbD-like 5934135 5934341 4.3 7929 + 6.98 0.09 1.55 0.16 10.42<br />
6348 RB11399 132 399 conserved hypothetical protein DUF393 6179167 6179565 6.5 15047 + 6.49 0.03 1.65 0.10 5.84<br />
216 RB375 311 936 conserved hypothetical protein 189235 190170 4.4 33465 + 5.81 0.17 1.81 0.05 2.63<br />
818 RB1522 387 1164 protein containing DUF1559 777494 778657 5.6 42272 + 5.95 0.12 1.85 0.25 13.51<br />
1788 RB3299 330 993 protein containing DUF1559 1693917 1692925 6.3 35808 - 6.29 0.12 1.72 0.21 12.00<br />
2407 RB4347 156 471 conserved hypothetical protein 2235735 2236205 4.7 17459 + 6.80 0.04 1.71 0.13 7.81<br />
2816 RB5001 103 312 hypothetical protein 2572354 2572665 10.3 11694 + 6.95 0.19 1.64 0.10 6.30<br />
2817 RB5003 498 1497 Rh-like protein/ammonium transporter AMT1.4 2572662 2574158 4 51180 + 6.63 0.21 2.11 0.04 1.91<br />
3346 RB5938 370 1113 hypothetical protein 3113484 3112372 5.6 41837 - 5.89 0.19 1.95 0.09 4.38<br />
3588 RB6379 153 462 hypothetical protein 3353122 3352661 5.8 17398 - 5.90 0.14 1.59 0.10 6.06<br />
3902 RB6912 55 168 secreted protein 3672793 3672960 9 5999 + 7.16 0.11 3.15 0.09 2.85<br />
3907 RB6920 355 1068 protein containing DUF1559 3676847 3677914 6.5 39241 + 6.75 0.05 1.82 0.05 2.58<br />
3909 RB6925 161 486 hypothetical protein 3678461 3677976 9.8 17641 - 7.10 0.08 2.01 0.15 7.43<br />
3910 RB6928 160 483 hypothetical protein 3678479 3678961 4.1 16965 + 6.22 0.10 2.84 0.12 4.34<br />
4791 RB8527 330 993 protein containing DUF1559 4549097 4550089 6.6 35645 + 6.03 0.14 2.15 0.23 10.56<br />
5122 RB9157 90 273 secreted protein 4899549 4899277 9.4 10178 - 6.25 0.13 1.52 0.15 9.67<br />
5143 RB9199 67 204 hypothetical protein 4918323 4918120 11.9 7371 - 5.67 0.05 1.77 0.25 14.04<br />
5618 RB10077 88 267 secreted protein 5436817 5437083 3.3 9650 + 6.00 0.18 1.66 0.18 10.96<br />
5968 RB10728 132 399 secreted protein 5831649 5832047 9.9 13833 + 5.73 0.07 2.48 0.15 6.16<br />
6043 RB10861 46 141 hypothetical protein 5896851 5896991 4.2 4833 + 7.46 0.08 2.60 0.16 6.18<br />
6067 RB10896 161 486 secreted protein 5917040 5916555 10 17034 - 5.66 0.04 2.08 0.01 0.68<br />
6084 RB10931 54 165 hypothetical protein 5933742 5933906 4.2 5888 + 6.11 0.16 1.56 0.20 13.15<br />
6098 RB10956 117 354 hypothetical protein 5940336 5940689 4.8 13013 + 5.64 0.12 2.00 0.39 19.31<br />
6099 RB10957 99 300 conserved hypothetical protein 5940708 5941007 5.6 11604 + 6.27 0.09 1.95 0.16 8.13<br />
6100 RB10958 158 477 hypothetical protein 5941015 5941491 5.4 18101 + 6.22 0.05 1.81 0.08 4.37<br />
6346 RB11396 36 111 conserved hypothetical protein 6178445 6178555 11.9 4104 + 6.89 0.20 1.87 0.24 12.96<br />
6347 RB11397 207 624 SOUL heme-binding protein 6178540 6179163 8.7 23072 + 6.51 0.04 1.78 0.10 5.41<br />
7202 RB12936 580 1743 conserved hypothetical protein 6965872 6964130 5.3 66248 - 5.77 0.16 1.58 0.23 14.63<br />
20min ID Locus AA Nuc Product Gene EC Start Stop IEP MW(kDa) Strand Intensity Stdev Ratio Stdev %<br />
6638 RB11919 671 2016 Dihydroxy-acid and 6-phosphogluconate dehydratase 6417713 6415698 5.3 70685 - 5.92 0.65 1.69 0.02 1.41<br />
317 RB548 633 1902 1,4-alpha-glucan branching enzyme glgB 2.4.1.18 306230 304329 4.4 72000 - 6.49 0.15 1.72 0.15 8.88<br />
212 RB370 553 1662 nitrate transporter substrate-binding protein 184428 186089 4.6 60742 + 6.85 0.11 1.98 0.14 7.26<br />
2817 RB5003 498 1497 Rh-like protein/ammonium transporter AMT1.4 2572662 2574158 4 51180 + 7.38 0.01 1.81 0.30 16.84<br />
3828 RB6780 557 1674 RNA polymerase sigma factor rpoD rpoD 3603381 3605054 9.6 64791 + 6.51 0.24 1.77 0.30 16.88<br />
6089 RB10939 62 189 conserved hypothetical protein 5934628 5934816 4.3 6627 + 6.88 0.10 1.82 0.43 23.74<br />
7362 RB13241 167 504 RNA polymerase ECF-type sigma factor 7103064 7102561 8.8 19488 - 6.30 0.47 2.28 0.57 24.79<br />
2463 RB4433 162 489 Ferritin and Dps 2277208 2277696 4.3 17757 + 6.62 0.10 2.19 0.43 19.68<br />
6086 RB10934 68 207 CsbD-like 5934135 5934341 4.3 7929 + 8.01 0.16 1.92 0.07 3.69<br />
6348 RB11399 132 399 Putative thiol-disulphide oxidoreductase DCC 6179167 6179565 6.5 15047 + 7.56 0.19 1.86 0.18 9.51<br />
216 RB375 311 936 conserved hypothetical protein 189235 190170 4.4 33465 + 6.61 0.22 2.03 0.32 15.68<br />
301 RB521 63 192 hypothetical protein 286399 286208 10.7 7040 - 6.30 0.11 1.78 0.21 11.95<br />
358 RB655 346 1041 conserved hypothetical protein, UCP012641 family 354993 353953 5.2 38936 - 6.30 0.16 1.57 0.25 15.93<br />
520 RB955 208 627 hypothetical protein 504140 503514 12.2 22575 - 5.89 0.60 1.66 0.32 19.09<br />
1151 RB2186 433 1302 ISXo8 transposase 1153993 1152692 9.4 49646 - 5.84 0.32 1.52 0.24 15.99<br />
1454 RB2682 83 252 hypothetical protein 1393781 1393530 7.4 8960 - 7.04 0.26 1.59 0.38 23.76<br />
1788 RB3299 330 993 protein containing DUF1559 1693917 1692925 6.3 35808 - 6.99 0.08 1.74 0.13 7.24<br />
2407 RB4347 156 471 conserved hypothetical protein 2235735 2236205 4.7 17459 + 7.59 0.21 1.69 0.05 2.80<br />
2465 RB4438 160 483 Pyridoxamine 5'-phosphate oxidase- 2278247 2278729 4.4 18043 + 6.61 0.15 1.70 0.09 5.36<br />
2649 RB4742 324 975 Universal stress protein uspE 2433589 2432615 4.7 35517 - 6.24 0.17 1.67 0.38 22.80<br />
2816 RB5001 103 312 hypothetical protein 2572354 2572665 10.3 11694 + 7.81 0.07 1.85 0.11 6.13<br />
2909 RB5167 44 135 hypothetical protein 2658500 2658366 12.4 4835 - 5.87 0.30 1.75 0.03 1.83<br />
2951 RB5238 73 222 hypothetical protein 2697775 2697996 10.6 7866 + 6.51 0.17 1.77 0.08 4.40<br />
3023 RB5371 109 330 hypothetical protein 2774829 2775158 7.5 12033 + 5.77 0.43 1.85 0.00 0.12<br />
3130 RB5551 663 1992 hypothetical protein 2889101 2891092 5.7 70932 + 6.15 0.18 1.50 0.30 20.00<br />
3304 RB5867 50 153 hypothetical protein 3077578 3077730 9.7 5871 + 6.06 0.56 1.90 0.17 9.08<br />
3346 RB5938 370 1113 hypothetical protein 3113484 3112372 5.6 41837 - 6.65 0.27 2.41 0.32 13.32<br />
3746 RB6637 50 153 hypothetical protein 3528317 3528469 10.1 5841 + 6.09 0.47 1.68 0.18 10.45<br />
3902 RB6912 55 168 secreted protein 3672793 3672960 9 5999 + 7.86 0.18 2.71 0.29 10.78<br />
3907 RB6920 355 1068 protein containing DUF1559 3676847 3677914 6.5 39241 + 7.75 0.27 2.09 0.24 11.40<br />
3910 RB6928 160 483 hypothetical protein 3678479 3678961 4.1 16965 + 7.15 0.32 3.25 0.38 11.58<br />
4157 RB7389 375 1128 Putative transposase 3953590 3954717 9.9 41895 + 6.19 0.59 1.95 0.42 21.65<br />
4595 RB8153 486 1461 conserved hypothetical protein 4359392 4357932 4.8 54199 - 5.90 0.20 1.61 0.19 11.86<br />
4606 RB8177 1318 3957 membrane protein 4371052 4375008 4.3 148031 + 6.21 0.30 2.31 0.28 12.30<br />
4608 RB8186 396 1191 secreted protein 4377386 4378576 4.5 43558 + 6.15 0.20 2.06 0.48 23.20<br />
4681 RB8325 296 891 hypothetical protein 4447029 4446139 6.8 32118 - 6.12 0.36 1.72 0.22 12.68<br />
4868 RB8679 477 1434 oxidoreductase 4620013 4618580 5.1 51903 - 5.83 0.25 1.56 0.13 8.46<br />
5038 RB8987 48 147 hypothetical protein 4790856 4790710 9.1 5309 - 6.06 0.41 1.93 0.16 8.10<br />
5143 RB9199 67 204 hypothetical protein 4918323 4918120 11.9 7371 - 6.63 0.13 2.12 0.40 19.00<br />
5525 RB9908 50 153 hypothetical protein 5339497 5339649 9.9 5867 + 6.02 0.61 1.79 0.15 8.42<br />
5618 RB10077 88 267 secreted protein 5436817 5437083 3.3 9650 + 6.97 0.32 2.04 0.40 19.69<br />
6043 RB10861 46 141 hypothetical protein 5896851 5896991 4.2 4833 + 8.20 0.04 2.38 0.48 20.12<br />
6051 RB10875 49 150 hypothetical protein 5901192 5901043 3.8 5020 - 5.91 0.37 1.62 0.18 11.32<br />
6085 RB10932 65 198 secreted protein 5933872 5934069 3.8 6983 + 7.40 0.16 1.73 0.09 5.17<br />
6098 RB10956 117 354 hypothetical protein 5940336 5940689 4.8 13013 + 6.71 0.31 2.90 0.65 22.39<br />
6100 RB10958 158 477 hypothetical protein 5941015 5941491 5.4 18101 + 7.03 0.08 1.91 0.41 21.51<br />
6177 RB11109 109 330 hypothetical protein 6022597 6022268 7.5 12033 - 5.85 0.23 1.51 0.27 17.86<br />
6347 RB11397 207 624 SOUL heme-binding protein 6178540 6179163 8.7 23072 + 7.46 0.20 1.87 0.39 20.98<br />
6577 RB11802 96 291 Transposase IS3/IS911 6361950 6362240 10.1 11124 + 6.03 0.14 1.50 0.16 10.74<br />
6585 RB11813 80 243 conserved hypothetical protein 6366331 6366573 13 9157 + 6.44 0.25 1.74 0.34 19.86<br />
6813 RB12240 50 153 conserved hypothetical protein 6594646 6594798 10.1 5841 + 6.09 0.34 1.58 0.30 18.73<br />
6897 RB12387 50 153 conserved hypothetical protein 6665894 6665742 10.1 5841 - 6.09 0.54 2.01 0.36 18.02<br />
7334 RB13190 50 153 conserved hypothetical protein 7083077 7083229 10.1 5841 + 6.16 0.22 1.75 0.35 20.07<br />
7351 RB13222 208 627 SOUL heme-binding protein 7094714 7094088 8.7 22867 - 6.27 0.40 2.95 0.04 1.26<br />
40min ID Locus AA Nuc Product Gene EC Start Stop IEP MW(kDa) Strand Intensity Stdev Ratio Stdev %<br />
1007 RB1909 166 501 Globin 992934 993434 4.5 18276 + 5.89 0.20 1.85 0.28 15.28<br />
5755 RB10327 127 384 Translation initiation factor SUI1 5582627 5583010 9.8 14121 + 6.88 0.24 1.56 0.33 21.23<br />
3828 RB6780 557 1674 RNA polymerase sigma factor rpoD rpoD 3603381 3605054 9.6 64791 + 6.13 0.15 1.55 0.06 4.05<br />
5571 RB9999 281 846 conserved hypothetical protein 5386828 5385983 4.6 29948 - 6.18 0.20 1.95 0.28 14.40<br />
5606 RB10049 217 654 RNA polymerase ECF-type sigma factor 5419507 5420160 10.1 25205 + 6.23 0.26 1.78 0.40 22.67<br />
2381 RB4299 96 291 Transposase IS3/IS911 2214533 2214823 9.9 11182 + 5.98 0.33 1.66 0.19 11.48<br />
3320 RB5888 96 291 Transposase IS3/IS911 3087636 3087346 10.1 11124 - 6.00 0.17 1.70 0.18 10.80<br />
6558 RB11749 96 291 Transposase IS3/IS911 6328064 6328354 10.1 11124 + 6.00 0.14 1.54 0.07 4.23<br />
6559 RB11750 292 879 integrase 6328357 6329235 10 33354 + 6.07 0.38 1.68 0.16 9.24<br />
6086 RB10934 68 207 CsbD-like 5934135 5934341 4.3 7929 + 7.34 0.17 1.57 0.22 13.90<br />
3605 RB6403 304 915 hypothetical protein 3369939 3370853 5.4 33688 + 6.54 0.15 1.57 0.22 13.73<br />
216 RB375 311 936 conserved hypothetical protein 189235 190170 4.4 33465 + 6.31 0.05 1.68 0.26 15.63<br />
301 RB521 63 192 hypothetical protein 286399 286208 10.7 7040 - 6.16 0.11 1.61 0.37 22.78<br />
2407 RB4347 156 471 conserved hypothetical protein 2235735 2236205 4.7 17459 + 7.09 0.11 1.93 0.08 3.98<br />
2431 RB4383 545 1638 decarboxylase 2258768 2257131 5.4 59839 - 6.33 0.06 1.61 0.33 20.28<br />
2465 RB4438 160 483 Pyridoxamine 5'-phosphate oxidase- 2278247 2278729 4.4 18043 + 6.23 0.05 1.79 0.28 15.52<br />
2503 RB4500 87 264 conserved hypothetical protein, secreted 2303645 2303908 3.5 9432 + 7.27 0.03 1.51 0.06 3.90<br />
2816 RB5001 103 312 hypothetical protein 2572354 2572665 10.3 11694 + 7.43 0.04 1.60 0.04 2.78<br />
2951 RB5238 73 222 hypothetical protein 2697775 2697996 10.6 7866 + 6.22 0.08 1.77 0.29 16.21<br />
3346 RB5938 370 1113 hypothetical protein 3113484 3112372 5.6 41837 - 6.48 0.02 2.44 0.32 12.99<br />
3902 RB6912 55 168 secreted protein 3672793 3672960 9 5999 + 7.32 0.24 1.94 0.44 22.83<br />
3909 RB6925 161 486 hypothetical protein 3678461 3677976 9.8 17641 - 7.53 0.14 2.19 0.25 11.49<br />
3910 RB6928 160 483 hypothetical protein 3678479 3678961 4.1 16965 + 6.83 0.27 2.53 0.42 16.57<br />
4606 RB8177 1318 3957 membrane protein 4371052 4375008 4.3 148031 + 5.83 0.36 1.68 0.08 4.91<br />
4608 RB8186 396 1191 secreted protein 4377386 4378576 4.5 43558 + 5.97 0.25 1.60 0.33 20.37<br />
4776 RB8505 439 1320 serine/threonine protein kinase related protein 4538446 4537127 4.7 48224 - 6.67 0.01 1.56 0.22 14.28<br />
5157 RB9230 107 324 hypothetical protein 4936673 4936996 9.9 11900 + 6.06 0.39 1.84 0.13 7.27<br />
5218 RB9347 68 207 hypothetical protein 5008600 5008394 9.9 7515 - 6.23 0.31 1.52 0.20 13.12
5524 RB9907 433 1302 ISXo8 transposase 5338185 5339486 9.4 49646 + 6.02 0.34 1.79 0.16 9.03<br />
5618 RB10077 88 267 secreted protein 5436817 5437083 3.3 9650 + 6.43 0.22 2.02 0.28 13.70<br />
6085 RB10932 65 198 secreted protein 5933872 5934069 3.8 6983 + 6.94 0.15 1.81 0.36 19.76<br />
6097 RB10954 143 432 hypothetical protein 5940749 5940318 10.4 16058 - 6.12 0.05 1.92 0.42 21.71<br />
6098 RB10956 117 354 hypothetical protein 5940336 5940689 4.8 13013 + 6.32 0.33 2.72 0.36 13.25<br />
6176 RB11107 225 678 secreted protein 6021425 6022102 6.5 25080 + 5.90 0.28 2.16 0.22 10.13<br />
6424 RB11504 72 219 conserved hypothetical protein 6221285 6221067 10.7 8212 - 6.15 0.14 2.56 0.09 3.69<br />
6455 RB11566 195 588 conserved hypothetical protein 6243111 6243698 10.8 22135 + 5.88 0.29 1.77 0.08 4.35<br />
6719 RB12067 107 324 conserved hypothetical protein 6510236 6510559 11.9 12169 + 5.77 0.32 1.57 0.29 18.43<br />
6817 RB12247 74 225 conserved hypothetical protein 6597048 6596824 6.3 8306 - 5.97 0.28 1.75 0.08 4.80<br />
6897 RB12387 50 153 conserved hypothetical protein 6665894 6665742 10.1 5841 - 5.70 0.27 1.87 0.41 22.16<br />
7124 RB12790 2396 7191 conserved hypothetical protein 6895351 6902541 4.4 261483 + 5.77 0.19 1.51 0.37 24.60<br />
60min ID Locus AA Nuc Product Gene EC Start Stop IEP MW(kDa) Strand Intensity Stdev Ratio Stdev %<br />
5144 RB9200 283 852 4-nitrophenylphosphatase PHO13 3.1.3.41 4919220 4918369 4.9 30413 - 6.09 0.18 1.99 0.21 10.63<br />
746 RB1392 340 1023 RNA polymerase sigma factor sigK 711705 710683 10.4 39326 - 7.02 0.14 2.09 0.02 0.82<br />
5571 RB9999 281 846 conserved hypothetical protein 5386828 5385983 4.6 29948 - 6.04 0.27 2.81 0.19 6.74<br />
5606 RB10049 217 654 RNA polymerase ECF-type sigma factor 5419507 5420160 10.1 25205 + 6.09 0.18 1.82 0.11 5.81<br />
6089 RB10939 62 189 conserved hypothetical protein 5934628 5934816 4.3 6627 + 6.49 0.16 1.96 0.17 8.64<br />
7362 RB13241 167 504 RNA polymerase ECF-type sigma factor 7103064 7102561 8.8 19488 - 5.75 0.13 2.83 0.29 10.20<br />
805 RB1502 133 402 two-component response regulator 769609 770010 5.6 14731 + 6.28 0.12 1.56 0.08 4.92<br />
6672 RB11976 237 714 DNA-binding response regulator mtrA 6455996 6455283 5.2 26398 - 5.95 0.18 1.72 0.02 1.21<br />
2463 RB4433 162 489 Ferritin and Dps 2277208 2277696 4.3 17757 + 6.09 0.15 1.95 0.10 5.09<br />
6425 RB11505 199 600 conserved hypothetical protein, secreted 6221829 6221230 7.5 21660 - 6.31 0.16 1.58 0.21 13.22<br />
5028 RB8970 540 1623 GroEL 4779905 4781527 4.8 57516 + 5.63 0.21 1.52 0.23 15.05<br />
6619 RB11878 168 507 peptide methionine sulfoxide reductase msrA msrA3 1.8.4.6 6396598 6397104 4.8 19100 + 6.06 0.18 1.61 0.06 3.67<br />
7103 RB12752 509 1530 HtrA-like serine protease 6871994 6873523 4.6 54858 + 5.88 0.16 1.52 0.04 2.66<br />
5783 RB10378 144 435 Thioredoxin trxA 5610497 5610063 4.6 15274 - 6.76 0.18 2.17 0.19 8.60<br />
5967 RB10727 276 831 manganese-containing catalase 5830775 5831605 5 30055 + 5.65 0.20 1.51 0.16 10.57<br />
745 RB1391 538 1617 cell division inhibitor- 710780 709164 7.8 59400 - 6.22 0.11 2.53 0.10 4.08<br />
6106 RB10971 303 912 Short-chain dehydrogenase/reductase SDR 5946406 5945495 9.8 32799 - 5.91 0.17 1.87 0.21 11.18<br />
6216 RB11174 469 1410 metallo-beta-lactamase family protein 6065046 6063637 4.8 51160 - 6.23 0.14 1.52 0.11 6.92<br />
6618 RB11877 465 1398 thiophene and furan oxidation protein 6395113 6396510 4.3 49500 + 6.23 0.15 1.60 0.19 11.63<br />
7369 RB13257 286 861 2-hydroxy-6-ketonona-2,4-dienedioic acid hydrolase bphD 3.7.1.- 7105590 7106450 4.6 32540 + 6.15 0.17 2.47 0.08 3.04<br />
6086 RB10934 68 207 CsbD-like 5934135 5934341 4.3 7929 + 7.36 0.17 1.54 0.06 4.13<br />
6348 RB11399 132 399 Putative thiol-disulphide oxidoreductase DCC 6179167 6179565 6.5 15047 + 7.13 0.16 2.22 0.08 3.69<br />
6266 RB11260 121 366 dnaK suppressor protein, 6107202 6106837 5.2 13639 - 5.70 0.20 2.02 0.47 23.38<br />
3605 RB6403 304 915 hypothetical protein 3369939 3370853 5.4 33688 + 6.46 0.14 1.92 0.09 4.67<br />
98 RB170 96 291 Transposase IS3/IS911 89333 89623 10.1 11124 + 5.89 0.22 1.54 0.12 7.92<br />
392 RB723 60 183 hypothetical protein 388051 388233 7.3 6624 + 5.86 0.21 1.82 0.09 5.20<br />
507 RB934 375 1128 Putative transposase 494203 493076 9.9 41881 - 5.68 0.19 1.86 0.11 6.10<br />
743 RB1389 152 459 hypothetical protein 708892 708434 11 16822 - 5.53 0.15 1.57 0.05 3.17<br />
744 RB1390 149 450 membrane protein 709167 708718 9.9 16437 - 6.09 0.10 1.71 0.18 10.49<br />
747 RB1394 78 237 hypothetical protein 711994 711758 10.1 8765 - 6.63 0.17 1.80 0.12 6.56<br />
748 RB1395 319 960 secreted protein 712097 713056 5.5 35212 + 6.66 0.12 1.84 0.06 3.26<br />
818 RB1522 387 1164 protein containing DUF1559 777494 778657 5.6 42272 + 6.14 0.23 1.82 0.15 8.06<br />
947 RB1789 243 732 conserved hypothetical protein 925861 926592 9.4 27028 + 6.04 0.19 1.57 0.08 5.06<br />
985 RB1872 38 117 hypothetical protein 966053 966169 12.3 4345 + 6.21 0.19 1.68 0.07 4.22<br />
2407 RB4347 156 471 conserved hypothetical protein 2235735 2236205 4.7 17459 + 7.21 0.18 1.74 0.11 6.41<br />
2425 RB4375 3056 9171 conserved hypothetical protein 2252103 2242933 3.5 296487 - 5.67 0.09 2.07 0.02 1.06<br />
2440 RB4397 55 168 protein containing DUF1560 2263976 2264143 10.5 5816 + 5.79 0.18 1.93 0.12 6.45<br />
2951 RB5238 73 222 hypothetical protein 2697775 2697996 10.6 7866 + 6.15 0.14 1.55 0.13 8.65<br />
3346 RB5938 370 1113 hypothetical protein 3113484 3112372 5.6 41837 - 6.46 0.15 2.01 0.12 6.12<br />
3472 RB6168 208 627 hypothetical protein 3236689 3237315 12.2 22575 + 5.63 0.19 1.66 0.25 15.21<br />
3588 RB6379 153 462 hypothetical protein 3353122 3352661 5.8 17398 - 6.42 0.18 2.20 0.28 12.57<br />
3902 RB6912 55 168 secreted protein 3672793 3672960 9 5999 + 7.20 0.18 2.54 0.06 2.29<br />
3907 RB6920 355 1068 protein containing DUF1559 3676847 3677914 6.5 39241 + 7.14 0.18 2.07 0.06 2.66<br />
3909 RB6925 161 486 hypothetical protein 3678461 3677976 9.8 17641 - 7.31 0.11 1.80 0.11 6.20<br />
3910 RB6928 160 483 hypothetical protein 3678479 3678961 4.1 16965 + 6.66 0.32 2.76 0.40 14.40<br />
4138 RB7341 7538 22617 HEMAGGLUTININ/HEMOLYSIN-RELATED PROTEIN 3913925 3936541 3.7 796559 + 5.67 0.25 1.93 0.37 19.00<br />
4157 RB7389 375 1128 Putative transposase 3953590 3954717 9.9 41895 + 5.94 0.14 1.87 0.15 7.88<br />
4465 RB7901 106 321 hypothetical protein 4221613 4221293 11.8 12088 - 6.16 0.21 1.66 0.03 1.82<br />
4606 RB8177 1318 3957 membrane protein 4371052 4375008 4.3 148031 + 5.48 0.15 1.68 0.35 20.62<br />
4714 RB8384 433 1302 ISXo8 transposase 4478623 4479924 9.4 49646 + 5.53 0.21 1.60 0.34 21.31<br />
4776 RB8505 439 1320 serine/threonine protein kinase related protein 4538446 4537127 4.7 48224 - 6.52 0.26 1.56 0.29 18.59<br />
4791 RB8527 330 993 protein containing DUF1559 4549097 4550089 6.6 35645 + 6.10 0.19 2.08 0.08 3.94<br />
5038 RB8987 48 147 hypothetical protein 4790856 4790710 9.1 5309 - 5.53 0.18 1.51 0.37 24.35<br />
5143 RB9199 67 204 hypothetical protein 4918323 4918120 11.9 7371 - 5.92 0.15 1.85 0.28 14.93<br />
5570 RB9998 277 834 ABC transporter ATP-binding protein 5385937 5385104 5.9 30617 - 7.08 0.18 1.53 0.09 5.61<br />
5618 RB10077 88 267 secreted protein 5436817 5437083 3.3 9650 + 6.35 0.24 2.06 0.21 10.40<br />
5750 RB10319 65 198 hypothetical protein 5577574 5577771 9.6 7480 + 6.21 0.16 1.98 0.09 4.48<br />
5753 RB10324 62 189 hypothetical protein 5580658 5580846 9 6582 + 6.11 0.19 1.57 0.14 8.88<br />
5968 RB10728 132 399 secreted protein 5831649 5832047 9.9 13833 + 5.90 0.24 2.67 0.18 6.84<br />
6043 RB10861 46 141 hypothetical protein 5896851 5896991 4.2 4833 + 7.80 0.18 2.14 0.07 3.26<br />
6067 RB10896 161 486 secreted protein 5917040 5916555 10 17034 - 6.00 0.19 2.90 0.17 5.87<br />
6084 RB10931 54 165 hypothetical protein 5933742 5933906 4.2 5888 + 6.55 0.18 2.17 0.15 6.74<br />
6085 RB10932 65 198 secreted protein 5933872 5934069 3.8 6983 + 6.79 0.17 1.89 0.10 5.05<br />
6097 RB10954 143 432 hypothetical protein 5940749 5940318 10.4 16058 - 5.82 0.18 2.08 0.18 8.56<br />
6098 RB10956 117 354 hypothetical protein 5940336 5940689 4.8 13013 + 6.14 0.24 2.94 0.07 2.26<br />
6099 RB10957 99 300 conserved hypothetical protein 5940708 5941007 5.6 11604 + 6.66 0.18 2.38 0.16 6.78<br />
6100 RB10958 158 477 hypothetical protein 5941015 5941491 5.4 18101 + 6.56 0.27 2.13 0.26 12.30<br />
6103 RB10966 60 183 Protein of unknown function, UPF0057 5942934 5942752 10.1 6793 - 6.37 0.17 1.62 0.11 6.98<br />
6176 RB11107 225 678 secreted protein 6021425 6022102 6.5 25080 + 5.82 0.25 2.42 0.38 15.63<br />
6217 RB11176 153 462 protein containing DUF442 6065605 6065144 4.8 17161 - 5.94 0.15 2.06 0.14 6.77<br />
6346 RB11396 36 111 conserved hypothetical protein 6178445 6178555 11.9 4104 + 7.35 0.11 1.69 0.10 5.79<br />
6347 RB11397 207 624 SOUL heme-binding protein 6178540 6179163 8.7 23072 + 6.96 0.20 2.16 0.14 6.66<br />
6406 RB11475 57 174 conserved hypothetical protein 6209468 6209641 4.7 5927 + 5.73 0.19 1.53 0.22 14.09<br />
6424 RB11504 72 219 conserved hypothetical protein 6221285 6221067 10.7 8212 - 5.99 0.20 2.10 0.23 10.74<br />
6455 RB11566 195 588 conserved hypothetical protein 6243111 6243698 10.8 22135 + 5.68 0.17 1.85 0.32 17.52<br />
6585 RB11813 80 243 conserved hypothetical protein 6366331 6366573 13 9157 + 6.25 0.16 1.64 0.24 14.58<br />
6673 RB11977 196 591 conserved hypothetical protein 6455958 6456548 9.5 21922 + 5.64 0.24 1.63 0.03 1.81<br />
6718 RB12066 135 408 conserved hypothetical protein 6510147 6510554 10 14924 + 6.42 0.10 1.79 0.21 11.65<br />
6719 RB12067 107 324 conserved hypothetical protein 6510236 6510559 11.9 12169 + 5.71 0.19 1.82 0.08 4.27<br />
6812 RB12239 433 1302 ISXo8 transposase 6593334 6594635 9.4 49646 + 5.57 0.11 1.54 0.08 5.21<br />
6817 RB12247 74 225 conserved hypothetical protein 6597048 6596824 6.3 8306 - 5.79 0.17 2.07 0.09 4.19<br />
6846 RB12297 169 510 conserved hypothetical protein 6615598 6616107 4.4 17963 + 6.12 0.11 1.70 0.08 4.61<br />
7124 RB12790 2396 7191 conserved hypothetical protein 6895351 6902541 4.4 261483 + 5.64 0.16 1.94 0.08 4.02<br />
7126 RB12797 57 174 conserved hypothetical protein 6905009 6905182 7.3 6555 + 5.94 0.26 2.17 0.05 2.15<br />
7202 RB12936 580 1743 conserved hypothetical protein 6965872 6964130 5.3 66248 - 6.18 0.18 2.01 0.26 13.05<br />
7203 RB12940 96 291 Transposase IS3/IS911 6966246 6966536 10.1 11124 + 6.00 0.15 1.57 0.05 3.08<br />
7278 RB13093 158 477 secreted protein 7025577 7025101 9.6 17298 - 6.16 0.28 1.70 0.20 11.47<br />
7281 RB13097 171 516 membrane protein 7027310 7026795 4.5 17420 - 6.39 0.22 1.56 0.27 17.42<br />
7361 RB13239 196 591 conserved hypothetical protein, membrane 7102571 7101981 9.5 22771 - 5.96 0.13 2.05 0.04 2.19<br />
300min ID Locus AA Nuc Product Gene EC Start Stop IEP MW(kDa) Strand Intensity Stdev Ratio Stdev %<br />
1961 RB3596 144 435 nitrogen fixation protein (NifU protein) 1868895 1869329 4.3 15958 + 5.97 0.21 1.74 0.25 14.28<br />
3637 RB6449 1932 5799 myosin-like membrane protein 3400438 3406236 4.7 214583 + 5.39 0.14 2.15 0.41 19.22<br />
1280 RB2394 236 711 conserved hypothetical protein 1245630 1244920 7 27126 - 5.96 0.25 1.74 0.20 11.35<br />
317 RB548 633 1902 1,4-alpha-glucan branching enzyme glgB 2.4.1.18 306230 304329 4.4 72000 - 5.90 0.24 1.50 0.11 7.12<br />
1622 RB2988 481 1446 Glycosidase, PH1107-related 1545296 1543851 5.7 54891 - 6.21 0.22 1.86 0.36 19.16<br />
1624 RB2991 391 1176 Glycosidase, PH1107-related 1548758 1547583 5.7 43690 - 5.74 0.20 1.91 0.13 6.80<br />
3104 RB5503 137 414 Ribosome-binding factor A rbfA 2847568 2847155 5.1 15253 - 5.95 0.22 1.61 0.12 7.23<br />
1218 RB2303 236 711 RNA POLYMERASE SIGMA-E FACTOR rpoE 1204509 1205219 6.4 26223 + 5.49 0.22 2.04 0.15 7.47<br />
5571 RB9999 281 846 conserved hypothetical protein 5386828 5385983 4.6 29948 - 6.15 0.24 2.10 0.22 10.60<br />
6089 RB10939 62 189 conserved hypothetical protein 5934628 5934816 4.3 6627 + 6.47 0.26 1.91 0.30 15.73<br />
3605 RB6403 304 915 hypothetical protein 3369939 3370853 5.4 33688 + 6.51 0.29 2.01 0.26 12.72<br />
1127 RB2146 139 420 Exonuclease VII, small subunit xseB 3.1.11.6 1136505 1136086 4.2 14778 - 5.91 0.25 1.78 0.08 4.30<br />
2381 RB4299 96 291 Transposase IS3/IS911 2214533 2214823 9.9 11182 + 5.52 0.16 1.63 0.06 3.61<br />
2463 RB4433 162 489 Ferritin and Dps 2277208 2277696 4.3 17757 + 5.69 0.31 3.36 0.23 6.92<br />
3588 RB6379 153 462 hypothetical protein 3353122 3352661 5.8 17398 - 6.34 0.21 1.68 0.16 9.28<br />
5728 RB10271 158 477 ribonuclease H rnhA 3.1.26.4 5552547 5552071 9.1 17665 - 5.68 0.33 1.91 0.10 5.49<br />
1623 RB2990 758 2277 mannosyltransferase 2.4.1.- 1547579 1545303 4.8 84426 - 5.90 0.23 1.80 0.08 4.63<br />
7150 RB12831 264 795 Glycosyltransferase 6921326 6920532 4.6 29501 - 6.13 0.24 2.12 0.23 10.91
1197 RB2268 282 849 peptide methionine sulfoxide reductase msrB 1.8.4.6 1191389 1190541 9.9 31115 - 6.22 0.27 2.06 0.36 17.21<br />
5028 RB8970 540 1623 GroEL 4779905 4781527 4.8 57516 + 5.68 0.26 1.60 0.14 9.06<br />
5649 RB10129 238 717 FKBP-type peptidyl-prolyl cis-trans isomerase fklB 5.2.1.8 5467429 5466713 4.9 26226 - 5.59 0.34 1.56 0.37 23.98<br />
5783 RB10378 144 435 Thioredoxin trxA 5610497 5610063 4.6 15274 - 6.65 0.21 1.51 0.03 1.96<br />
6675 RB11981 317 954 ABC transporter (substrate-binding protein) 6459460 6460413 4.8 34461 + 6.03 0.28 1.58 0.14 9.08<br />
1278 RB2386 528 1587 RND efflux membrane fusion protein 1244320 1242734 6 58921 - 6.19 0.26 1.74 0.12 6.76<br />
5570 RB9998 277 834 ABC transporter ATP-binding protein 5385937 5385104 5.9 30617 - 6.72 0.32 1.50 0.30 19.95<br />
7369 RB13257 286 861 2-hydroxy-6-ketonona-2,4-dienedioic acid hydrolase bphD 3.7.1.- 7105590 7106450 4.6 32540 + 6.08 0.21 1.53 0.09 6.00<br />
2438 RB4395 170 513 carboxymuconolactone decarboxylase 4.1.1.44 2262023 2262535 4.7 18791 + 5.96 0.25 1.63 0.34 21.08<br />
2488 RB4474 168 507 CsbD-like 2295048 2295554 8.8 18451 + 7.12 0.24 1.96 0.07 3.70<br />
5413 RB9699 697 2094 Rhs element Vgr protein 5226252 5228345 4.2 76047 + 5.94 0.32 1.63 0.37 22.47<br />
6266 RB11260 121 366 dnaK suppressor protein, 6107202 6106837 5.2 13639 - 5.95 0.18 1.82 0.06 3.37<br />
10 RB17 164 495 protein containing DUF1589 8251 7757 11.5 18498 - 5.48 0.29 1.97 0.30 15.11<br />
358 RB655 346 1041 conserved hypothetical protein, UCP012641 family 354993 353953 5.2 38936 - 6.01 0.30 2.32 0.15 6.66<br />
470 RB865 542 1629 hypothetical protein 458731 460359 3.1 54518 + 5.77 0.28 1.90 0.18 9.68<br />
507 RB934 375 1128 Putative transposase 494203 493076 9.9 41881 - 5.57 0.27 1.84 0.03 1.58<br />
947 RB1789 243 732 conserved hypothetical protein 925861 926592 9.4 27028 + 5.83 0.28 1.66 0.23 13.88<br />
1454 RB2682 83 252 hypothetical protein 1393781 1393530 7.4 8960 - 6.29 0.30 1.77 0.34 19.07<br />
1490 RB2745 41 126 hypothetical protein 1431275 1431400 9.9 4387 + 5.88 0.19 1.57 0.32 20.19<br />
1528 RB2829 38 117 hypothetical protein 1467448 1467564 4.1 3898 + 6.42 0.28 1.63 0.38 23.43<br />
1744 RB3228 962 2889 protein containing DUF1585 1656635 1659523 4.6 107564 + 5.75 0.27 1.52 0.35 23.38<br />
2351 RB4256 358 1077 conserved hypothetical protein, secreted 2180303 2181379 4.5 37091 + 6.62 0.30 1.89 0.24 12.95<br />
2407 RB4347 156 471 conserved hypothetical protein 2235735 2236205 4.7 17459 + 6.97 0.30 2.61 0.23 8.75<br />
2426 RB4376 493 1482 catalase katA 1.11.1.6 2253743 2252262 5.5 56567 - 5.89 0.24 1.69 0.30 17.74<br />
2431 RB4383 545 1638 decarboxylase 2258768 2257131 5.4 59839 - 5.90 0.18 2.06 0.13 6.20<br />
2461 RB4429 89 270 conserved hypothetical protein 2275996 2276265 4.9 10034 + 6.59 0.30 2.29 0.37 16.02<br />
2465 RB4438 160 483 Pyridoxamine 5'-phosphate oxidase- 2278247 2278729 4.4 18043 + 5.63 0.27 1.94 0.22 11.08<br />
2485 RB4468 162 489 hypothetical protein 2294104 2293616 4.6 16380 - 6.30 0.19 1.66 0.28 16.98<br />
2489 RB4476 128 387 hypothetical protein 2295557 2295943 11.4 14037 + 6.94 0.23 1.68 0.26 15.76<br />
2499 RB4493 83 252 hypothetical protein 2302374 2302123 10.2 8978 - 6.95 0.23 1.83 0.21 11.45<br />
2503 RB4500 87 264 conserved hypothetical protein, secreted 2303645 2303908 3.5 9432 + 7.27 0.34 2.49 0.22 8.92<br />
2509 RB4510 49 150 hypothetical protein 2307657 2307508 9 5554 - 5.82 0.15 1.60 0.09 5.77<br />
2951 RB5238 73 222 hypothetical protein 2697775 2697996 10.6 7866 + 6.04 0.22 2.57 0.10 4.03<br />
3118 RB5523 91 276 hypothetical protein 2860984 2860709 6.3 9939 - 5.87 0.24 2.52 0.19 7.62<br />
3346 RB5938 370 1113 hypothetical protein 3113484 3112372 5.6 41837 - 6.27 0.23 3.07 0.35 11.47<br />
3449 RB6131 102 309 hypothetical protein 3223482 3223790 12.1 12343 + 6.20 0.29 1.65 0.30 18.20<br />
3647 RB6467 252 759 phosphoglycerate/bisphosphoglycerate mutase family protein 5.4.2.1 3418620 3419378 5.9 27957 + 6.12 0.29 1.92 0.44 22.94<br />
4157 RB7389 375 1128 Putative transposase 3953590 3954717 9.9 41895 + 5.61 0.29 2.40 0.14 5.62<br />
4288 RB7603 836 2511 outer membrane efflux protein 4091070 4088560 5.1 91203 - 5.90 0.32 1.53 0.25 16.49<br />
4318 RB7651 44 135 hypothetical protein 4112517 4112383 6.7 4749 - 5.71 0.29 1.77 0.16 8.86<br />
4465 RB7901 106 321 hypothetical protein 4221613 4221293 11.8 12088 - 6.02 0.28 2.41 0.17 6.98<br />
4704 RB8365 90 273 membrane protein 4463903 4463631 11.7 9405 - 6.14 0.21 1.78 0.23 13.06<br />
4727 RB8409 97 294 hypothetical protein 4495547 4495840 8.9 10905 + 5.46 0.26 1.54 0.30 19.20<br />
5157 RB9230 107 324 hypothetical protein 4936673 4936996 9.9 11900 + 5.88 0.22 1.64 0.20 12.26<br />
5218 RB9347 68 207 hypothetical protein 5008600 5008394 9.9 7515 - 6.12 0.23 1.67 0.28 16.62<br />
5550 RB9955 452 1359 secreted protein containing DUF1552 5363605 5362247 5.6 50315 - 6.08 0.21 2.27 0.08 3.61<br />
5565 RB9986 112 339 hypothetical protein 5380717 5380379 11.1 12671 - 5.85 0.28 1.75 0.15 8.40<br />
5567 RB9990 53 162 hypothetical protein 5381822 5381983 10.1 6033 + 5.16 0.28 2.14 0.59 27.35<br />
5618 RB10077 88 267 secreted protein 5436817 5437083 3.3 9650 + 6.38 0.28 2.17 0.38 17.62<br />
6053 RB10877 453 1362 membrane protein 5905128 5903767 4.3 48882 - 5.49 0.30 1.55 0.10 6.46<br />
6084 RB10931 54 165 hypothetical protein 5933742 5933906 4.2 5888 + 6.97 0.25 1.92 0.02 0.81<br />
6085 RB10932 65 198 secreted protein 5933872 5934069 3.8 6983 + 6.96 0.27 2.15 0.17 7.78<br />
6097 RB10954 143 432 hypothetical protein 5940749 5940318 10.4 16058 - 5.83 0.22 1.66 0.33 20.04<br />
6098 RB10956 117 354 hypothetical protein 5940336 5940689 4.8 13013 + 6.42 0.32 3.24 0.38 11.68<br />
6099 RB10957 99 300 conserved hypothetical protein 5940708 5941007 5.6 11604 + 7.07 0.20 2.05 0.21 10.37<br />
6176 RB11107 225 678 secreted protein 6021425 6022102 6.5 25080 + 6.14 0.24 2.37 0.33 14.03<br />
6406 RB11475 57 174 conserved hypothetical protein 6209468 6209641 4.7 5927 + 5.81 0.26 1.68 0.15 9.04<br />
6430 RB11515 74 225 conserved hypothetical protein 6224420 6224644 11.7 8687 + 5.34 0.26 2.74 0.34 12.52<br />
6455 RB11566 195 588 conserved hypothetical protein 6243111 6243698 10.8 22135 + 5.62 0.25 1.84 0.33 17.86<br />
6585 RB11813 80 243 conserved hypothetical protein 6366331 6366573 13 9157 + 5.73 0.28 1.82 0.22 12.24<br />
6606 RB11855 101 306 conserved hypothetical protein 6383765 6383460 12.5 11384 - 5.97 0.23 2.42 0.10 4.22<br />
6637 RB11918 134 405 protein containing DUF971 6415701 6415297 6.1 14675 - 5.89 0.32 2.14 0.11 5.02<br />
6719 RB12067 107 324 conserved hypothetical protein 6510236 6510559 11.9 12169 + 5.87 0.35 2.73 0.28 10.34<br />
7128 RB12800 156 471 conserved hypothetical protein 6908240 6908710 9.5 16259 + 5.79 0.20 1.92 0.41 21.19<br />
7149 RB12830 102 309 conserved hypothetical protein 6920508 6920200 10.9 11753 - 5.86 0.22 2.11 0.42 19.84<br />
7278 RB13093 158 477 secreted protein 7025577 7025101 9.6 17298 - 5.88 0.25 1.59 0.12 7.33<br />
7351 RB13222 208 627 SOUL heme-binding protein 7094714 7094088 8.7 22867 - 5.81 0.19 3.86 0.31 7.96<br />
7354 RB13228 116 351 conserved hypothetical protein 7097952 7098302 10.4 13570 + 5.95 0.21 1.85 0.13 7.09
Cold repressed<br />
10min ID Locus AA Nuc Product Gene EC Start Stop IEP MW(kDa) Strand Intensity Stdev Ratio Stdev %<br />
3188 RB5653 500 1503 NADH-glutamate synthase small chain 2948867 2947365 5 54985 - 6.14 0.12 -1.45 0.05 3.16<br />
4429 RB7838 89 270 Ribosomal protein S19/S15 4195376 4195645 10.8 9943 + 6.90 0.03 -1.81 0.13 7.04<br />
4430 RB7839 119 360 Ribosomal protein L22/L17 4195706 4196065 11 13471 + 6.88 0.04 -1.45 0.13 8.77<br />
4431 RB7840 236 711 30S ribosomal protein S3 rpsC 4196161 4196871 10.4 26575 + 6.79 0.12 -1.84 0.15 7.94<br />
4432 RB7841 138 417 Ribosomal protein L16 4196810 4197226 11.1 15313 + 6.53 0.24 -2.59 0.16 6.23<br />
4434 RB7849 108 327 Ribosomal protein S17 4197700 4198026 10 12074 + 6.48 0.01 -2.94 0.16 5.46<br />
4435 RB7850 122 369 Ribosomal protein L14b/L23e 4198081 4198449 11 13526 + 6.51 0.04 -2.84 0.11 3.75<br />
4437 RB7852 196 591 50S ribosomal protein L5 rplE 4198935 4199525 10.4 22118 + 6.13 0.18 -2.06 0.12 5.99<br />
4438 RB7854 61 186 Ribosomal protein S14 4199624 4199809 11.8 6962 + 6.56 0.21 -2.33 0.18 7.89<br />
4440 RB7856 181 546 50S ribosomal protein L6 rplF 4200306 4200851 10 19733 + 6.53 0.19 -2.13 0.36 17.02<br />
4441 RB7857 149 450 Ribosomal protein L18P/L5E 4200854 4201303 11.6 16541 + 6.17 0.06 -2.22 0.10 4.46<br />
4442 RB7859 177 534 Ribosomal protein S5 4201351 4201884 10.6 18782 + 6.44 0.21 -1.47 0.15 10.41<br />
4459 RB7894 398 1197 translation elongation factor EF-Tu 4217484 4218680 5.2 43184 + 7.32 0.20 -1.95 0.05 2.43<br />
4463 RB7899 141 426 50S ribosomal protein L11 rplK 4220226 4220651 9.6 15063 + 6.31 0.11 -1.63 0.21 12.89<br />
7143 RB12821 117 354 Ribosomal protein L19 6916558 6916205 11.1 13347 - 6.10 0.12 -1.52 0.10 6.80<br />
7145 RB12824 146 441 Ribosomal protein S16 6917743 6917303 5.3 15885 - 6.57 0.20 -1.42 0.09 6.57<br />
7154 RB12839 225 678 Ribosomal protein L1 6921934 6922611 9.8 24159 + 7.34 0.04 -1.60 0.08 4.93<br />
179 RB314 309 930 malonyl CoA-acyl carrier protein transacylase fabD 2.3.1.39 156968 157897 4.4 32349 + 6.76 0.04 -1.10 0.21 18.82<br />
7140 RB12816 257 774 glutamine amidotransferase or related peptidase 6914710 6915483 4.5 28223 + 6.55 0.21 -1.34 0.04 3.00<br />
839 RB1563 136 411 hypothetical protein 798934 799344 10.2 14882 + 6.67 0.13 -1.37 0.25 18.20<br />
2312 RB4194 53 162 hypothetical protein 2156685 2156524 11.4 5728 - 7.26 0.05 -1.34 0.14 10.14<br />
2781 RB4951 95 288 hypothetical protein 2547498 2547785 12.1 10953 + 7.10 0.03 -1.25 0.22 17.32<br />
2782 RB4954 166 501 secreted protein 2547861 2548361 10.2 18478 + 6.26 0.07 -1.53 0.03 2.22<br />
2894 RB5138 58 177 hypothetical protein 2641024 2640848 12.4 6711 - 5.90 0.08 -1.13 0.15 13.38<br />
3049 RB5415 62 189 hypothetical protein 2798737 2798925 12.3 7098 + 6.51 0.05 -1.60 0.09 5.66<br />
3063 RB5435 78 237 hypothetical protein 2817808 2818044 7.4 8596 + 6.26 0.21 -1.23 0.25 20.69<br />
3239 RB5745 130 393 hypothetical protein 3001234 3000842 10.7 13522 - 7.04 0.14 -1.99 0.20 9.98<br />
4015 RB7116 59 180 hypothetical protein 3779586 3779765 11.7 6458 + 6.79 0.22 -1.72 0.06 3.21<br />
4314 RB7646 62 189 hypothetical protein 4111735 4111923 10.5 6950 + 6.93 0.07 -1.60 0.22 13.74<br />
4461 RB7897 152 459 Protein secE/sec61-gamma protein 4218945 4219403 9.6 16638 + 5.93 0.03 -1.34 0.22 16.26<br />
4486 RB7939 105 318 hypothetical protein 4238296 4238613 11 12019 + 6.02 0.12 -1.09 0.20 18.42<br />
4611 RB8191 72 219 hypothetical protein 4382096 4382314 12 8590 + 7.52 0.02 -1.19 0.15 12.30<br />
4749 RB8457 113 342 hypothetical protein 4518458 4518117 11.5 12525 - 7.00 0.15 -1.15 0.18 15.79<br />
4862 RB8670 47 144 hypothetical protein 4614842 4614985 11.1 5371 + 7.17 0.14 -1.25 0.16 13.05<br />
4880 RB8700 58 177 hypothetical protein 4630224 4630400 4.8 6268 + 6.46 0.07 -1.34 0.11 7.84<br />
5885 RB10579 101 306 secreted protein 5739254 5738949 12.4 11507 - 6.36 0.11 -1.93 0.14 7.02<br />
5887 RB10581 384 1155 secreted protein containing DUF1559 5739353 5740507 6.2 41431 + 6.05 0.07 -1.27 0.09 7.01<br />
6371 RB11439 93 282 conserved hypothetical protein 6197993 6198274 10.6 10906 + 8.02 0.12 -1.21 0.12 10.28<br />
6867 RB12329 110 333 conserved hypothetical protein, membrane 6634234 6634566 4 11862 + 7.05 0.21 -1.04 0.14 13.82<br />
7139 RB12815 43 132 conserved hypothetical protein 6914656 6914525 10.8 4782 - 6.65 0.17 -1.40 0.10 7.18<br />
7141 RB12818 163 492 conserved hypothetical protein 6915989 6915498 11.7 18010 - 6.96 0.15 -1.36 0.12 9.11<br />
20min ID Locus AA Nuc Product Gene EC Start Stop IEP MW(kDa) Strand Intensity Stdev Ratio Stdev %<br />
2359 RB4269 282 849 glutamic acid specific endopeptidase, GSE=serine endopeptidase 2185049 2184201 5.6 31283 - 6.31 0.24 -1.70 0.12 6.81<br />
3188 RB5653 500 1503 NADH-glutamate synthase small chain 2948867 2947365 5 54985 - 6.55 0.10 -1.53 0.27 17.79<br />
5375 RB9629 330 993 acyl-lipid delta-9 desaturase ADS2 1.14.19.- 5184385 5183393 6.5 37640 - 5.83 0.10 -1.32 0.01 0.39<br />
4429 RB7838 89 270 Ribosomal protein S19/S15 4195376 4195645 10.8 9943 + 7.49 0.13 -1.89 0.16 8.59<br />
4430 RB7839 119 360 Ribosomal protein L22/L17 4195706 4196065 11 13471 + 7.61 0.09 -1.63 0.21 12.76<br />
4431 RB7840 236 711 30S ribosomal protein S3 rpsC 4196161 4196871 10.4 26575 + 7.39 0.10 -2.09 0.20 9.38<br />
4432 RB7841 138 417 Ribosomal protein L16 4196810 4197226 11.1 15313 + 7.36 0.15 -2.41 0.09 3.76<br />
4434 RB7849 108 327 Ribosomal protein S17 4197700 4198026 10 12074 + 7.21 0.12 -2.85 0.20 6.86<br />
4435 RB7850 122 369 Ribosomal protein L14b/L23e 4198081 4198449 11 13526 + 7.18 0.08 -2.68 0.25 9.24<br />
4436 RB7851 115 348 50S ribosomal protein L24 rplX 4198529 4198876 10.5 12484 + 7.04 0.18 -2.68 0.20 7.40<br />
4437 RB7852 196 591 50S ribosomal protein L5 rplE 4198935 4199525 10.4 22118 + 6.54 0.27 -1.78 0.42 23.64<br />
4438 RB7854 61 186 Ribosomal protein S14 4199624 4199809 11.8 6962 + 7.10 0.14 -2.24 0.16 7.04<br />
4439 RB7855 133 402 Ribosomal protein S8 4199839 4200240 10.4 15217 + 6.73 0.18 -1.78 0.32 17.95<br />
4440 RB7856 181 546 50S ribosomal protein L6 rplF 4200306 4200851 10 19733 + 7.11 0.14 -1.99 0.16 8.09<br />
4441 RB7857 149 450 Ribosomal protein L18P/L5E 4200854 4201303 11.6 16541 + 6.94 0.13 -1.66 0.08 4.82<br />
4442 RB7859 177 534 Ribosomal protein S5 4201351 4201884 10.6 18782 + 7.06 0.16 -1.32 0.12 8.85<br />
4459 RB7894 398 1197 translation elongation factor EF-Tu 4217484 4218680 5.2 43184 + 7.84 0.04 -2.37 0.22 9.33<br />
4463 RB7899 141 426 50S ribosomal protein L11 rplK 4220226 4220651 9.6 15063 + 6.97 0.02 -1.61 0.23 14.06<br />
7143 RB12821 117 354 Ribosomal protein L19 6916558 6916205 11.1 13347 - 6.93 0.17 -1.15 0.23 19.82<br />
7145 RB12824 146 441 Ribosomal protein S16 6917743 6917303 5.3 15885 - 7.37 0.16 -1.28 0.09 6.64<br />
7154 RB12839 225 678 Ribosomal protein L1 6921934 6922611 9.8 24159 + 7.86 0.28 -1.75 0.22 12.35<br />
7156 RB12842 140 423 Ribosomal protein L7/L12 6923401 6923823 4.3 14489 + 6.57 0.31 -1.37 0.32 23.28<br />
181 RB318 81 246 Acyl carrier protein acpP 158928 159173 3.7 9018 + 6.89 0.10 -1.08 0.12 11.17<br />
2065 RB3751 371 1116 NAD-dependent epimerase/dehydratase 1938953 1940068 5.5 40775 + 6.12 0.37 -1.10 0.20 18.07<br />
3442 RB6114 690 2073 UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase murE 6.3.2.13 3208897 3210969 5 73645 + 6.36 0.14 -1.02 0.12 11.50<br />
3890 RB6895 401 1206 Glycosyl transferase, family 4 3666358 3665153 6.6 42272 - 5.96 0.09 -1.15 0.27 23.93<br />
2244 RB4061 488 1467 Peptidase A24A, prepilin type IV:Peptidase A24A, N-terminal 2095498 2096964 9.7 54251 + 6.18 0.22 -1.06 0.13 12.70<br />
2472 RB4454 739 2220 Flagellin A flaA 2283201 2285420 4 76924 + 6.30 0.15 -1.02 0.12 12.20<br />
1680 RB3109 411 1236 Methylamine utilization protein mauG mauG 1611359 1612594 5.5 45393 + 6.28 0.14 -1.19 0.02 1.37<br />
1850 RB3403 491 1476 arylsulfatase precursor arsA 3.1.6.8 1756892 1755417 5.5 55139 - 6.37 0.13 -1.18 0.21 17.64<br />
2363 RB4273 518 1557 iron-dependent peroxidase 1.1.1.- 2193431 2191875 5.1 57672 - 6.04 0.30 -1.22 0.29 23.61<br />
3246 RB5758 300 903 Integral membrane protein TerC 3014379 3013477 5 32769 - 6.23 0.20 -1.05 0.11 10.84<br />
4953 RB8834 331 996 Cation efflux protein 4697452 4698447 5.2 36348 + 5.80 0.09 -1.01 0.18 17.50<br />
597 RB1134 537 1614 NADH dehydrogenase subunit F 585258 583645 9.4 57512 - 6.77 0.12 -2.61 0.04 1.54<br />
1644 RB3032 477 1434 Major facilitator superfamily MFS_1 1567813 1569246 9.8 52109 + 6.36 0.10 -1.48 0.00 0.04<br />
7168 RB12861 309 930 Oligopeptide transport system permease protein OppB. oppB 6932929 6932000 9.5 33415 - 5.67 0.12 -1.30 0.07 5.05<br />
7140 RB12816 257 774 glutamine amidotransferase or related peptidase 6914710 6915483 4.5 28223 + 7.09 0.11 -1.57 0.15 9.45<br />
594 RB1129 895 2688 conserved hypothetical protein 583271 580584 5.8 99242 - 6.37 0.21 -2.46 0.56 22.67<br />
658 RB1233 206 621 30S ribosomal protein S4 rpsD 629490 628870 11.2 23193 - 7.38 0.09 -1.26 0.06 4.63<br />
4308 RB7634 773 2322 Abortive infection protein 4106267 4108588 6 84772 + 5.79 0.07 -1.23 0.25 20.18<br />
3336 RB5918 252 759 biotin-protein ligase 3098715 3097957 5.2 26935 - 6.01 0.13 -1.25 0.06 4.71<br />
3208 RB5695 288 867 carbon-nitrogen hydrolase family 2971401 2972267 4.5 31927 + 6.00 0.15 -1.13 0.12 10.55<br />
2222 RB4014 473 1422 Cip2 2075607 2074186 4.9 52531 - 6.24 0.18 -1.02 0.20 19.09<br />
38 RB62 131 396 conserved hypothetical protein 25588 25983 10.4 15483 + 5.88 0.08 -1.42 0.25 17.44<br />
1332 RB2479 273 822 conserved hypothetical protein 1296683 1295862 5.5 30436 - 6.45 0.10 -1.49 0.07 5.00<br />
1904 RB3495 218 657 conserved hypothetical protein 1808101 1808757 5 23739 + 5.97 0.31 -1.12 0.19 16.90<br />
1946 RB3569 123 372 conserved hypothetical protein 1854728 1854357 7.5 13875 - 6.23 0.26 -1.23 0.25 20.21<br />
2260 RB4097 733 2202 conserved hypothetical protein 2108244 2110445 6.2 83188 + 6.18 0.37 -1.57 0.34 21.71<br />
3726 RB6596 230 693 conserved hypothetical protein 3507067 3506375 10.2 25422 - 6.02 0.12 -1.01 0.23 22.87<br />
5313 RB9520 532 1599 conserved hypothetical protein 5114495 5112897 4.4 59231 - 5.95 0.21 -1.45 0.11 7.61<br />
5353 RB9588 268 807 conserved hypothetical protein 5156821 5156015 5.6 29455 - 5.75 0.11 -1.17 0.27 22.74<br />
6319 RB11343 93 282 conserved hypothetical protein 6154696 6154977 12.2 10760 + 6.01 0.03 -1.32 0.17 12.76<br />
6539 RB11707 83 252 conserved hypothetical protein 6312937 6313188 9.8 9109 + 5.91 0.05 -1.12 0.23 20.87<br />
6642 RB11925 76 231 conserved hypothetical protein 6419855 6420085 12.2 8248 + 5.92 0.14 -1.03 0.26 25.03<br />
6818 RB12250 104 315 conserved hypothetical protein 6597248 6597562 11.9 12364 + 6.04 0.13 -1.02 0.22 21.63<br />
6904 RB12396 57 174 conserved hypothetical protein 6670870 6670697 11.3 6680 - 6.01 0.17 -1.07 0.24 22.30<br />
7009 RB12581 174 525 conserved hypothetical protein 6780597 6780073 9.9 19433 - 5.92 0.26 -1.45 0.29 20.26<br />
7139 RB12815 43 132 conserved hypothetical protein 6914656 6914525 10.8 4782 - 7.28 0.18 -1.43 0.13 9.00<br />
7141 RB12818 163 492 conserved hypothetical protein 6915989 6915498 11.7 18010 - 7.47 0.17 -1.17 0.24 20.70<br />
7153 RB12837 65 198 conserved hypothetical protein 6921732 6921929 9.8 7318 + 7.96 0.22 -1.05 0.17 16.15<br />
7367 RB13251 64 195 conserved hypothetical protein 7104552 7104746 11.2 7416 + 5.70 0.09 -1.02 0.09 8.52<br />
1542 RB2852 240 723 conserved hypothetical protein, membrane 1482871 1482149 9.4 26727 - 6.19 0.05 -1.21 0.14 11.38<br />
2387 RB4305 380 1143 conserved hypothetical protein, membrane 2219737 2218595 9.6 40925 - 6.21 0.29 -1.19 0.20 16.72<br />
3247 RB5761 258 777 conserved hypothetical protein, membrane 3015202 3014426 10.9 27944 - 6.16 0.15 -1.22 0.18 14.52<br />
6416 RB11490 181 546 conserved hypothetical protein, membrane 6215558 6216103 10.3 20670 + 5.96 0.12 -1.23 0.07 5.90<br />
409 RB767 311 936 conserved hypothetical protein, secreted 411420 412355 5.7 34002 + 6.03 0.18 -1.33 0.31 23.56<br />
2935 RB5210 423 1272 conserved hypothetical protein, secreted 2687633 2688904 6.5 47488 + 5.91 0.35 -1.15 0.19 16.64<br />
2334 RB4224 396 1191 DNA polymerase IV dinB 2.7.7.7 2164703 2165893 10 44202 + 6.30 0.13 -1.11 0.27 23.93<br />
6819 RB12251 567 1704 DNA-directed RNA polymerase specialized sigma factor 6599262 6597559 9.4 59396 - 5.97 0.10 -1.23 0.10 7.92<br />
3528 RB6276 105 318 Histone-like bacterial DNA-binding protein 3297504 3297187 10.4 11299 - 8.40 0.12 -1.30 0.08 5.97<br />
37 RB61 58 177 hypothetical protein 25378 25554 9.6 6492 + 6.09 0.23 -1.01 0.21 21.19<br />
108 RB187 35 108 hypothetical protein 95474 95367 9.4 3992 - 6.08 0.14 -1.26 0.17 13.59<br />
111 RB192 56 171 hypothetical protein 100254 100084 10.1 6457 - 6.00 0.18 -1.10 0.11 9.80<br />
133 RB241 47 144 hypothetical protein 124403 124546 11.4 5298 + 6.06 0.12 -1.03 0.14 13.35<br />
178 RB313 93 282 hypothetical protein 156945 156664 11.6 10508 - 6.99 0.15 -1.02 0.21 20.58<br />
182 RB319 95 288 hypothetical protein 159334 159621 10.2 10505 + 6.89 0.13 -1.49 0.18 11.82<br />
202 RB354 51 156 hypothetical protein 177535 177380 12 5703 - 6.40 0.31 -1.07 0.22 20.74<br />
246 RB424 50 153 hypothetical protein 226220 226068 4.3 5601 - 6.07 0.29 -1.14 0.14 12.55<br />
274 RB469 44 135 hypothetical protein 252425 252291 4.2 5513 - 5.80 0.15 -1.00 0.25 25.03
276 RB471 181 546 hypothetical protein 253264 252719 10.8 20224 - 5.98 0.13 -1.25 0.05 4.08<br />
283 RB484 58 177 hypothetical protein 262633 262809 10.6 6245 + 5.78 0.09 -1.21 0.19 15.87<br />
348 RB625 68 207 hypothetical protein 339703 339497 5 7713 - 5.64 0.04 -1.09 0.28 25.80<br />
416 RB779 86 261 hypothetical protein 419045 418785 4.5 9321 - 5.78 0.05 -1.09 0.00 0.39<br />
449 RB825 117 354 hypothetical protein 434184 433831 7.8 13302 - 6.30 0.23 -1.05 0.11 10.84<br />
691 RB1291 97 294 hypothetical protein 656684 656977 10.4 10290 + 5.98 0.16 -1.38 0.14 10.21<br />
839 RB1563 136 411 hypothetical protein 798934 799344 10.2 14882 + 7.47 0.05 -1.09 0.07 6.19<br />
1012 RB1922 102 309 hypothetical protein 998917 998609 10.9 11658 - 6.23 0.06 -1.04 0.25 24.35<br />
1016 RB1931 68 207 hypothetical protein 1013831 1013625 11.4 7493 - 6.10 0.37 -1.33 0.10 7.31<br />
1200 RB2274 130 393 hypothetical protein 1193331 1192939 10 14362 - 6.27 0.13 -1.18 0.08 6.83<br />
1220 RB2306 41 126 hypothetical protein 1206782 1206907 9 4759 + 6.35 0.23 -1.36 0.05 3.43<br />
1227 RB2317 162 489 hypothetical protein 1211131 1210643 7.7 18020 - 6.06 0.14 -1.00 0.11 10.84<br />
1251 RB2350 37 114 hypothetical protein 1220890 1221003 11.9 4181 + 6.08 0.21 -1.18 0.09 7.53<br />
1252 RB2351 133 402 hypothetical protein 1220991 1221392 10.4 15178 + 6.04 0.28 -1.12 0.28 24.95<br />
1297 RB2425 102 309 hypothetical protein 1260766 1261074 11.1 11189 + 6.26 0.09 -1.06 0.14 13.59<br />
1344 RB2495 43 132 hypothetical protein 1303912 1304043 11.7 4921 + 6.26 0.27 -1.03 0.19 18.80<br />
1543 RB2853 42 129 hypothetical protein 1482846 1482974 11.8 5043 + 6.12 0.15 -1.09 0.16 14.70<br />
1734 RB3205 76 231 hypothetical protein 1652079 1651849 11.3 9082 - 5.91 0.29 -1.00 0.12 11.71<br />
1761 RB3256 140 423 hypothetical protein 1676574 1676996 4.5 16074 + 6.45 0.14 -1.29 0.24 18.25<br />
1776 RB3277 221 666 hypothetical protein 1686552 1685887 10.4 24222 - 6.21 0.17 -1.23 0.10 8.23<br />
1825 RB3362 87 264 hypothetical protein 1728546 1728283 11.9 10017 - 6.44 0.33 -1.29 0.23 17.84<br />
1828 RB3366 78 237 hypothetical protein 1730380 1730616 5.6 9481 + 6.05 0.33 -1.11 0.07 6.75<br />
1833 RB3372 115 348 hypothetical protein 1733666 1734013 10 12916 + 6.09 0.22 -1.17 0.06 5.18<br />
1844 RB3394 36 111 hypothetical protein 1752044 1752154 10.5 4239 + 6.37 0.21 -1.94 0.04 2.07<br />
1846 RB3398 67 204 hypothetical protein 1753293 1753496 11.4 7537 + 5.97 0.14 -1.08 0.13 12.46<br />
1847 RB3399 65 198 hypothetical protein 1753650 1753453 9.7 7418 - 6.27 0.19 -1.19 0.15 12.44<br />
1854 RB3407 89 270 hypothetical protein 1759935 1759666 10.5 10136 - 6.24 0.33 -1.34 0.29 21.53<br />
1943 RB3566 64 195 hypothetical protein 1854080 1854274 9.4 7400 + 6.13 0.18 -1.24 0.13 10.17<br />
2006 RB3660 94 285 hypothetical protein 1900854 1900570 9.4 10411 - 6.51 0.47 -1.23 0.29 23.38<br />
2025 RB3688 53 162 hypothetical protein 1910629 1910468 9.3 5981 - 6.24 0.28 -1.21 0.10 8.42<br />
2048 RB3719 141 426 hypothetical protein 1927500 1927075 10.8 16330 - 6.49 0.20 -1.25 0.21 16.69<br />
2098 RB3813 75 228 hypothetical protein 1975188 1975415 4.5 8522 + 6.18 0.32 -1.21 0.26 21.52<br />
2139 RB3880 82 249 hypothetical protein 2006953 2006705 10.7 9535 - 6.13 0.11 -1.13 0.06 5.02<br />
2184 RB3953 857 2574 hypothetical protein 2048784 2046211 5.2 93881 - 6.29 0.26 -1.40 0.36 25.65<br />
2200 RB3981 161 486 hypothetical protein 2058000 2058485 4.1 18283 + 6.29 0.17 -1.17 0.11 9.03<br />
2216 RB4002 483 1452 hypothetical protein 2065341 2066792 4.6 53759 + 6.28 0.18 -1.01 0.23 23.32<br />
2258 RB4093 45 138 hypothetical protein 2107600 2107737 10 5285 + 6.21 0.15 -1.04 0.08 7.24<br />
2287 RB4145 90 273 hypothetical protein 2131933 2131661 12 10727 - 6.91 0.26 -1.07 0.19 17.63<br />
2298 RB4165 721 2166 hypothetical protein 2139632 2141797 4.5 77994 + 6.19 0.22 -1.07 0.21 20.09<br />
2312 RB4194 53 162 hypothetical protein 2156685 2156524 11.4 5728 - 8.07 0.12 -1.09 0.03 2.67<br />
2350 RB4255 204 615 hypothetical protein 2180806 2180192 10.3 21582 - 9.54 0.03 -1.63 0.20 12.54<br />
2416 RB4358 123 372 hypothetical protein 2239474 2239103 6.5 13459 - 6.33 0.30 -1.49 0.27 17.98<br />
2424 RB4373 109 330 hypothetical protein 2242863 2242534 4.8 12263 - 6.41 0.14 -1.15 0.10 8.36<br />
2444 RB4402 53 162 hypothetical protein 2265340 2265501 10.4 5980 + 6.17 0.27 -1.36 0.26 19.15<br />
2528 RB4547 422 1269 hypothetical protein 2330116 2328848 7.6 46274 - 6.07 0.21 -1.20 0.29 23.86<br />
2552 RB4589 62 189 hypothetical protein 2351649 2351461 9.4 7014 - 5.90 0.12 -1.17 0.14 12.25<br />
2553 RB4590 74 225 hypothetical protein 2352024 2351800 10.1 8330 - 9.62 0.01 -1.89 0.26 13.47<br />
2596 RB4657 123 372 hypothetical protein 2381865 2382236 12.2 13398 + 6.50 0.24 -1.69 0.17 10.05<br />
2647 RB4740 50 153 hypothetical protein 2431814 2431966 11.4 5598 + 6.35 0.24 -1.03 0.04 4.02<br />
2687 RB4801 96 291 hypothetical protein 2459502 2459212 9.8 10561 - 6.11 0.34 -1.00 0.05 4.69<br />
2746 RB4897 184 555 hypothetical protein 2519505 2518951 10.9 20446 - 6.03 0.32 -1.33 0.19 14.60<br />
2781 RB4951 95 288 hypothetical protein 2547498 2547785 12.1 10953 + 7.46 0.34 -1.52 0.24 16.10<br />
2864 RB5088 57 174 hypothetical protein 2617615 2617442 6.7 6093 - 5.91 0.24 -1.04 0.15 14.51<br />
2963 RB5261 126 381 hypothetical protein 2713712 2713332 7.4 13761 - 6.00 0.28 -1.00 0.23 23.41<br />
3049 RB5415 62 189 hypothetical protein 2798737 2798925 12.3 7098 + 6.88 0.37 -1.87 0.32 17.33<br />
3063 RB5435 78 237 hypothetical protein 2817808 2818044 7.4 8596 + 6.96 0.20 -1.10 0.05 4.92<br />
3132 RB5555 54 165 hypothetical protein 2891274 2891438 6.2 5971 + 6.30 0.34 -1.16 0.22 18.56<br />
3239 RB5745 130 393 hypothetical protein 3001234 3000842 10.7 13522 - 7.72 0.11 -2.40 0.06 2.62<br />
3389 RB6010 53 162 hypothetical protein 3149911 3150072 11.9 5744 + 6.04 0.27 -1.25 0.11 8.98<br />
3466 RB6158 142 429 hypothetical protein 3233532 3233104 6 15746 - 6.57 0.19 -1.29 0.10 7.70<br />
3476 RB6174 69 210 hypothetical protein 3239734 3239525 10.6 7870 - 6.11 0.12 -1.06 0.21 19.64<br />
3485 RB6193 62 189 hypothetical protein 3246096 3245908 11.4 6970 - 6.81 0.25 -1.45 0.09 5.96<br />
3720 RB6587 50 153 hypothetical protein 3501747 3501595 7.5 5579 - 6.06 0.12 -1.08 0.10 8.84<br />
3742 RB6632 55 168 hypothetical protein 3524963 3525130 5.6 6193 + 5.97 0.24 -1.11 0.03 2.61<br />
3780 RB6699 47 144 hypothetical protein 3559781 3559924 11.1 5290 + 5.99 0.22 -1.09 0.16 14.69<br />
3820 RB6766 55 168 hypothetical protein 3597367 3597534 12 6133 + 6.21 0.23 -1.23 0.29 23.68<br />
3860 RB6838 55 168 hypothetical protein 3636563 3636730 4.8 5823 + 5.76 0.19 -1.16 0.18 15.70<br />
3867 RB6849 101 306 hypothetical protein 3642537 3642232 12.8 12002 - 6.25 0.37 -1.17 0.08 6.77<br />
3871 RB6856 62 189 hypothetical protein 3645613 3645425 9.9 6382 - 5.75 0.12 -1.19 0.15 12.19<br />
3921 RB6951 1120 3363 hypothetical protein 3693377 3690015 4.6 123187 - 6.22 0.14 -1.03 0.26 25.58<br />
3971 RB7042 91 276 hypothetical protein 3743971 3744246 10.4 10835 + 6.19 0.11 -1.22 0.08 6.26<br />
4009 RB7109 53 162 hypothetical protein 3775707 3775546 6.7 5850 - 5.86 0.15 -1.54 0.21 13.93<br />
4015 RB7116 59 180 hypothetical protein 3779586 3779765 11.7 6458 + 7.48 0.04 -1.60 0.16 10.27<br />
4278 RB7589 86 261 hypothetical protein 4080238 4079978 12.1 9472 - 5.85 0.13 -1.00 0.18 18.08<br />
4313 RB7645 77 234 hypothetical protein 4111716 4111483 10.3 8725 - 5.73 0.14 -1.45 0.25 17.05<br />
4314 RB7646 62 189 hypothetical protein 4111735 4111923 10.5 6950 + 7.38 0.33 -1.81 0.19 10.35<br />
4552 RB8070 59 180 hypothetical protein 4319538 4319717 4.5 6478 + 5.84 0.21 -1.27 0.30 23.31<br />
4568 RB8098 71 216 hypothetical protein 4337334 4337119 10.8 7950 - 9.57 0.02 -1.70 0.24 14.33<br />
4578 RB8119 142 429 hypothetical protein 4346427 4346855 10.1 15659 + 5.95 0.11 -1.21 0.24 20.07<br />
4677 RB8319 37 114 hypothetical protein 4442421 4442308 4.4 4328 - 5.79 0.08 -1.58 0.20 12.94<br />
4755 RB8468 88 267 hypothetical protein 4520799 4520533 11.9 9719 - 6.80 0.13 -1.00 0.09 8.75<br />
4830 RB8594 41 126 hypothetical protein 4581645 4581770 9.2 4509 + 6.31 0.04 -1.23 0.14 11.58<br />
4861 RB8669 37 114 hypothetical protein 4614557 4614670 11.5 4395 + 9.53 0.16 -2.04 0.37 18.16<br />
5245 RB9392 175 528 hypothetical protein 5038107 5037580 4.9 19499 - 5.92 0.16 -1.16 0.07 5.93<br />
5253 RB9410 138 417 hypothetical protein 5048582 5048166 10 15146 - 5.78 0.13 -1.01 0.25 25.13<br />
5280 RB9460 79 240 hypothetical protein 5078860 5078621 10.3 8991 - 9.45 0.13 -1.41 0.33 23.24<br />
5445 RB9767 97 294 hypothetical protein 5264348 5264641 8.9 10547 + 5.89 0.07 -1.14 0.18 15.87<br />
5988 RB10755 41 126 hypothetical protein 5839576 5839451 12.7 5083 - 5.93 0.17 -1.05 0.08 7.51<br />
6280 RB11287 75 228 hypothetical protein 6118405 6118632 9.1 8478 + 6.04 0.07 -1.17 0.11 9.69<br />
2047 RB3718 535 1608 Major facilitator superfamily MFS_1 1927114 1925507 4.6 57372 - 6.04 0.20 -1.16 0.12 10.66<br />
22 RB37 160 483 membrane protein 18328 17846 5.6 17283 - 6.16 0.41 -1.28 0.16 12.77<br />
43 RB73 98 297 membrane protein 28727 29023 10.1 11150 + 5.79 0.09 -1.16 0.05 3.97<br />
70 RB125 297 894 membrane protein 54571 53678 6 32981 - 5.82 0.11 -1.12 0.08 7.05<br />
547 RB1015 222 669 membrane protein 531723 532391 5.9 24319 + 5.88 0.08 -1.09 0.13 11.53<br />
1105 RB2105 470 1413 membrane protein 1114012 1112600 9.6 51853 - 6.47 0.07 -1.76 0.22 12.29<br />
1147 RB2179 199 600 membrane protein 1149988 1150587 10.5 22376 + 6.14 0.25 -1.03 0.22 21.09<br />
1446 RB2667 236 711 membrane protein 1387198 1386488 10.7 25698 - 6.30 0.23 -1.08 0.16 15.03<br />
1597 RB2951 174 525 membrane protein 1523616 1523092 9.3 20061 - 6.13 0.40 -1.38 0.20 14.80<br />
1668 RB3067 153 462 membrane protein 1582850 1582389 7.6 17200 - 6.06 0.19 -1.05 0.22 20.90<br />
1701 RB3147 168 507 membrane protein 1624840 1624334 8.7 18759 - 6.31 0.18 -1.05 0.14 13.51<br />
1950 RB3575 152 459 membrane protein 1858779 1859237 10.1 17195 + 6.22 0.21 -1.33 0.05 3.64<br />
2664 RB4766 130 393 membrane protein 2442991 2442599 9.7 14452 - 6.05 0.21 -1.13 0.08 6.78<br />
2884 RB5118 140 423 membrane protein 2630572 2630150 9.1 16117 - 6.08 0.21 -1.24 0.28 22.54<br />
2964 RB5262 95 288 membrane protein 2713742 2714029 6.3 10993 + 6.16 0.21 -1.48 0.30 20.54<br />
3169 RB5622 175 528 membrane protein 2930538 2930011 4.3 19919 - 5.94 0.23 -1.13 0.07 6.44<br />
4282 RB7596 175 528 membrane protein 4084008 4084535 7.5 19347 + 5.76 0.07 -1.01 0.22 21.89<br />
4718 RB8389 939 2820 membrane protein 4484734 4481915 10.1 105627 - 5.93 0.14 -1.07 0.08 7.33<br />
5469 RB9806 165 498 membrane protein 5280590 5281087 10.2 18166 + 5.74 0.01 -1.03 0.18 17.85<br />
2083 RB3779 1030 3093 membrane-bound sensor and histidine kinase response regulator hybrid 1957809 1954717 4.8 114806 - 6.10 0.25 -1.14 0.16 13.93<br />
1352 RB2503 515 1548 O-antigen flippase wzx 1311056 1309509 10 55740 - 6.13 0.32 -1.17 0.12 10.30<br />
2774 RB4937 369 1110 oxidoreductase 2540086 2541195 5 39670 + 6.14 0.31 -1.06 0.09 8.68<br />
3278 RB5820 355 1068 oxidoreductase 3050638 3051705 4.9 39347 + 5.97 0.27 -1.07 0.27 25.63<br />
3430 RB6092 361 1086 Peptidase M50 3.4.24.- 3192387 3193472 9.6 39370 + 6.19 0.25 -1.28 0.30 23.11<br />
6979 RB12521 726 2181 Peptidase M50 3.4.24.- 6744279 6746459 10.7 80320 + 5.80 0.27 -1.15 0.11 9.37<br />
3183 RB5645 556 1671 periplasmic serine protease 2944822 2943152 5.2 60661 - 5.73 0.18 -1.13 0.10 8.95<br />
3092 RB5478 131 396 pilus protein 2834459 2834064 4.2 14264 - 5.75 0.16 -1.01 0.06 6.24<br />
562 RB1052 391 1176 protein containing DUF1559 548353 549528 5.5 42194 + 5.71 0.09 -1.05 0.19 18.49<br />
3744 RB6634 365 1098 protein containing DUF1559 3525785 3526882 5.3 40217 + 6.11 0.12 -1.17 0.14 11.89<br />
1659 RB3056 90 273 protein containing DUF1560 1579951 1579679 10.3 10144 - 6.19 0.24 -1.05 0.12 11.62<br />
377 RB693 260 783 protein containing DUF1568 371190 370408 6.8 30735 - 5.91 0.16 -1.23 0.28 22.47<br />
516 RB951 234 705 protein containing DUF1596 502459 501755 12.3 25583 - 6.67 0.17 -1.51 0.23 15.00<br />
3688 RB6530 237 714 protein containing DUF480 3468520 3467807 5.4 25750 - 5.96 0.12 -1.01 0.17 16.62<br />
4461 RB7897 152 459 Protein secE/sec61-gamma protein 4218945 4219403 9.6 16638 + 6.69 0.13 -1.06 0.18 16.59<br />
3151 RB5593 1290 3873 protein serine/threonine kinase 2.7.1.37 2913054 2909182 4.8 144451 - 5.92 0.14 -1.09 0.05 4.66<br />
2369 RB4283 499 1500 PvdD/PvdJ 2201462 2199963 5.7 56411 - 6.28 0.25 -1.35 0.25 18.22
4428 RB7837 286 861 Ribosomal protein L2 4194481 4195341 11.8 31108 + 7.62 0.02 -1.12 0.24 21.29<br />
4016 RB7117 181 546 Ribosomal protein L35 3780322 3779777 11.4 19816 - 7.52 0.07 -1.01 0.15 15.12<br />
280 RB479 1101 3306 secreted protein 259174 255869 4.9 123358 - 5.94 0.31 -1.11 0.21 19.40<br />
1074 RB2051 367 1104 secreted protein 1079177 1080280 4.3 40652 + 6.21 0.16 -1.40 0.20 14.10<br />
1649 RB3042 291 876 secreted protein 1575622 1574747 6 32861 - 6.15 0.27 -1.12 0.25 21.89<br />
1967 RB3603 344 1035 secreted protein 1875461 1874427 4.6 37999 - 6.23 0.27 -1.69 0.27 16.14<br />
2017 RB3675 742 2229 secreted protein 1904552 1906780 8.4 82951 + 6.31 0.34 -1.34 0.29 21.98<br />
2782 RB4954 166 501 secreted protein 2547861 2548361 10.2 18478 + 6.90 0.18 -1.50 0.05 3.40<br />
5205 RB9324 267 804 secreted protein 4992474 4993277 6.7 29463 + 5.99 0.19 -1.60 0.20 12.27<br />
5885 RB10579 101 306 secreted protein 5739254 5738949 12.4 11507 - 7.13 0.10 -1.42 0.21 14.89<br />
7321 RB13168 81 246 secreted protein 7068345 7068590 7.4 8735 + 5.70 0.36 -1.08 0.04 3.84<br />
130 RB229 585 1758 secreted protein containing DUF1551 122782 121025 4.5 62454 - 6.23 0.23 -1.18 0.18 15.61<br />
6899 RB12390 239 720 secreted protein containing DUF1555 6668063 6667344 4.1 25406 - 6.47 0.11 -1.00 0.09 9.39<br />
3197 RB5674 237 714 serine/threonine protein phosphatase 2962887 2962174 5.6 26548 - 5.93 0.23 -1.15 0.18 15.70<br />
3291 RB5841 670 2013 serine/threonine protein phosphatase family 3061924 3063936 5.4 73664 + 6.07 0.06 -1.03 0.16 15.11<br />
2729 RB4870 1081 3246 transporter, AcrB/D/F family 2502527 2499282 5 116198 - 6.17 0.23 -1.18 0.16 13.91<br />
4255 RB7557 327 984 von Willebrand factor type A domain protein 4058936 4059919 4.9 35328 + 5.82 0.18 -1.27 0.20 16.04<br />
40 min ID Locus AA Nuc Product Gene EC Start Stop IEP MW(kDa) Strand Intensity Stdev Ratio Stdev %<br />
1967 RB3603 344 1035 secreted protein 1875461 1874427 4.6 37999 - 6.40 0.26 -1.06 0.11 10.27<br />
2359 RB4269 282 849 glutamic acid specific endopeptidase, GSE=serine endopeptidase 2185049 2184201 5.6 31283 - 6.50 0.23 -1.09 0.23 21.37<br />
4429 RB7838 89 270 Ribosomal protein S19/S15 4195376 4195645 10.8 9943 + 7.30 0.15 -1.36 0.14 10.34<br />
4430 RB7839 119 360 Ribosomal protein L22/L17 4195706 4196065 11 13471 + 7.37 0.05 -1.15 0.12 10.52<br />
4431 RB7840 236 711 30S ribosomal protein S3 rpsC 4196161 4196871 10.4 26575 + 7.29 0.04 -1.24 0.12 9.94<br />
4432 RB7841 138 417 Ribosomal protein L16 4196810 4197226 11.1 15313 + 7.16 0.10 -1.88 0.15 7.95<br />
4434 RB7849 108 327 Ribosomal protein S17 4197700 4198026 10 12074 + 6.95 0.05 -2.41 0.29 12.13<br />
4435 RB7850 122 369 Ribosomal protein L14b/L23e 4198081 4198449 11 13526 + 6.93 0.09 -2.15 0.27 12.53<br />
4436 RB7851 115 348 50S ribosomal protein L24 rplX 4198529 4198876 10.5 12484 + 6.59 0.15 -2.22 0.19 8.70<br />
4438 RB7854 61 186 Ribosomal protein S14 4199624 4199809 11.8 6962 + 6.88 0.21 -1.75 0.12 6.99<br />
4439 RB7855 133 402 Ribosomal protein S8 4199839 4200240 10.4 15217 + 6.49 0.14 -1.47 0.16 10.70<br />
4440 RB7856 181 546 50S ribosomal protein L6 rplF 4200306 4200851 10 19733 + 6.83 0.19 -1.75 0.25 14.56<br />
4442 RB7859 177 534 Ribosomal protein S5 4201351 4201884 10.6 18782 + 6.87 0.14 -1.06 0.11 10.48<br />
4459 RB7894 398 1197 translation elongation factor EF-Tu 4217484 4218680 5.2 43184 + 7.68 0.10 -2.02 0.02 1.12<br />
4463 RB7899 141 426 50S ribosomal protein L11 rplK 4220226 4220651 9.6 15063 + 6.81 0.13 -1.23 0.11 9.19<br />
7154 RB12839 225 678 Ribosomal protein L1 6921934 6922611 9.8 24159 + 7.46 0.19 -1.69 0.21 12.59<br />
7156 RB12842 140 423 Ribosomal protein L7/L12 6923401 6923823 4.3 14489 + 6.20 0.42 -1.52 0.32 21.07<br />
179 RB314 309 930 malonyl CoA-acyl carrier protein transacylase fabD 2.3.1.39 156968 157897 4.4 32349 + 7.15 0.05 -1.01 0.11 11.11<br />
597 RB1134 537 1614 NADH dehydrogenase subunit F 585258 583645 9.4 57512 - 6.39 0.26 -2.17 0.32 14.59<br />
7140 RB12816 257 774 glutamine amidotransferase or related peptidase 6914710 6915483 4.5 28223 + 6.87 0.03 -1.26 0.18 13.84<br />
182 RB319 95 288 hypothetical protein 159334 159621 10.2 10505 + 6.86 0.23 -1.24 0.18 14.18<br />
516 RB951 234 705 protein containing DUF1596 502459 501755 12.3 25583 - 6.69 0.12 -1.10 0.02 2.10<br />
1220 RB2306 41 126 hypothetical protein 1206782 1206907 9 4759 + 6.49 0.54 -1.05 0.19 18.04<br />
1847 RB3399 65 198 hypothetical protein 1753650 1753453 9.7 7418 - 6.57 0.36 -1.00 0.21 20.78<br />
2553 RB4590 74 225 hypothetical protein 2352024 2351800 10.1 8330 - 9.62 0.00 -1.53 0.18 11.55<br />
2596 RB4657 123 372 hypothetical protein 2381865 2382236 12.2 13398 + 6.59 0.16 -1.14 0.15 13.30<br />
2781 RB4951 95 288 hypothetical protein 2547498 2547785 12.1 10953 + 7.15 0.37 -1.36 0.28 20.46<br />
2789 RB4966 84 255 hypothetical protein 2552311 2552057 11.3 10016 - 9.59 0.04 -1.43 0.08 5.78<br />
3045 RB5409 97 294 hypothetical protein 2794195 2794488 12.7 10898 + 8.37 0.31 -1.07 0.09 7.97<br />
3239 RB5745 130 393 hypothetical protein 3001234 3000842 10.7 13522 - 7.49 0.10 -1.67 0.11 6.76<br />
3820 RB6766 55 168 hypothetical protein 3597367 3597534 12 6133 + 6.43 0.34 -1.04 0.17 15.98<br />
4015 RB7116 59 180 hypothetical protein 3779586 3779765 11.7 6458 + 7.38 0.15 -1.04 0.05 4.73<br />
4314 RB7646 62 189 hypothetical protein 4111735 4111923 10.5 6950 + 7.17 0.38 -1.56 0.24 15.23<br />
4315 RB7647 73 222 hypothetical protein 4111931 4112152 7.4 8269 + 9.38 0.33 -1.13 0.14 12.09<br />
4568 RB8098 71 216 hypothetical protein 4337334 4337119 10.8 7950 - 9.47 0.17 -1.10 0.22 19.69<br />
4861 RB8669 37 114 hypothetical protein 4614557 4614670 11.5 4395 + 9.54 0.14 -1.52 0.18 11.96<br />
5205 RB9324 267 804 secreted protein 4992474 4993277 6.7 29463 + 6.31 0.03 -1.02 0.04 3.78<br />
5280 RB9460 79 240 hypothetical protein 5078860 5078621 10.3 8991 - 9.35 0.30 -1.16 0.08 7.23<br />
6781 RB12193 36 111 conserved hypothetical protein 6575977 6575867 7.5 3719 - 6.50 0.06 -1.05 0.11 10.74<br />
7141 RB12818 163 492 conserved hypothetical protein 6915989 6915498 11.7 18010 - 7.35 0.29 -1.06 0.06 5.40<br />
60 min ID Locus AA Nuc Product Gene EC Start Stop IEP MW(kDa) Strand I Stdev R Stdev %<br />
2359 RB4269 282 849 glutamic acid specific endopeptidase, GSE=serine endopeptidase 2185049 2184201 5.6 31283 - 6.39 0.04 -1.13 0.26 22.71<br />
3188 RB5653 500 1503 NADH-glutamate synthase small chain 2948867 2947365 5 54985 - 6.55 0.10 -1.31 0.11 8.11<br />
4429 RB7838 89 270 Ribosomal protein S19/S15 4195376 4195645 10.8 9943 + 7.39 0.18 -1.37 0.15 11.01<br />
4430 RB7839 119 360 Ribosomal protein L22/L17 4195706 4196065 11 13471 + 7.36 0.17 -1.19 0.04 3.32<br />
4431 RB7840 236 711 30S ribosomal protein S3 rpsC 4196161 4196871 10.4 26575 + 7.27 0.27 -1.45 0.08 5.26<br />
4432 RB7841 138 417 Ribosomal protein L16 4196810 4197226 11.1 15313 + 7.13 0.25 -2.14 0.07 3.39<br />
4434 RB7849 108 327 Ribosomal protein S17 4197700 4198026 10 12074 + 6.99 0.15 -2.38 0.27 11.32<br />
4435 RB7850 122 369 Ribosomal protein L14b/L23e 4198081 4198449 11 13526 + 6.94 0.15 -2.53 0.14 5.35<br />
4436 RB7851 115 348 50S ribosomal protein L24 rplX 4198529 4198876 10.5 12484 + 6.66 0.23 -2.36 0.22 9.20<br />
4437 RB7852 196 591 50S ribosomal protein L5 rplE 4198935 4199525 10.4 22118 + 6.64 0.16 -1.97 0.34 17.17<br />
4438 RB7854 61 186 Ribosomal protein S14 4199624 4199809 11.8 6962 + 6.96 0.25 -2.21 0.09 4.21<br />
4439 RB7855 133 402 Ribosomal protein S8 4199839 4200240 10.4 15217 + 6.46 0.20 -1.95 0.21 11.02<br />
4440 RB7856 181 546 50S ribosomal protein L6 rplF 4200306 4200851 10 19733 + 6.84 0.10 -2.05 0.19 9.42<br />
4441 RB7857 149 450 Ribosomal protein L18P/L5E 4200854 4201303 11.6 16541 + 6.42 0.25 -1.76 0.31 17.58<br />
4442 RB7859 177 534 Ribosomal protein S5 4201351 4201884 10.6 18782 + 6.87 0.18 -1.38 0.16 11.82<br />
4463 RB7899 141 426 50S ribosomal protein L11 rplK 4220226 4220651 9.6 15063 + 6.72 0.23 -1.57 0.20 12.83<br />
7145 RB12824 146 441 Ribosomal protein S16 6917743 6917303 5.3 15885 - 7.00 0.18 -1.25 0.16 12.86<br />
7154 RB12839 225 678 Ribosomal protein L1 6921934 6922611 9.8 24159 + 7.67 0.16 -1.69 0.09 5.18<br />
7156 RB12842 140 423 Ribosomal protein L7/L12 6923401 6923823 4.3 14489 + 6.05 0.24 -1.57 0.08 5.31<br />
4459 RB7894 398 1197 translation elongation factor EF-Tu 4217484 4218680 5.2 43184 + 7.62 0.20 -2.38 0.03 1.45<br />
179 RB314 309 930 malonyl CoA-acyl carrier protein transacylase fabD 2.3.1.39 156968 157897 4.4 32349 + 7.09 0.12 -1.15 0.12 10.27<br />
597 RB1134 537 1614 NADH dehydrogenase subunit F 585258 583645 9.4 57512 - 6.47 0.17 -2.37 0.17 7.12<br />
7140 RB12816 257 774 glutamine amidotransferase or related peptidase 6914710 6915483 4.5 28223 + 6.86 0.18 -1.40 0.04 2.67<br />
594 RB1129 895 2688 conserved hypothetical protein 583271 580584 5.8 99242 - 6.34 0.14 -2.46 0.25 10.12<br />
182 RB319 95 288 hypothetical protein 159334 159621 10.2 10505 + 6.91 0.11 -1.34 0.09 7.04<br />
416 RB779 86 261 hypothetical protein 419045 418785 4.5 9321 - 5.77 0.02 -1.02 0.16 15.42<br />
595 RB1132 54 165 hypothetical protein 583247 583411 10 6091 + 6.04 0.13 -1.05 0.25 24.11<br />
1843 RB3393 60 183 secreted protein 1751865 1752047 12.3 7156 + 6.61 0.04 -1.10 0.12 11.12<br />
1844 RB3394 36 111 hypothetical protein 1752044 1752154 10.5 4239 + 6.43 0.04 -1.27 0.21 16.72<br />
2210 RB3994 191 576 hypothetical protein 2061291 2061866 4.1 20739 + 6.27 0.02 -1.00 0.17 17.02<br />
2287 RB4145 90 273 hypothetical protein 2131933 2131661 12 10727 - 6.67 0.10 -1.11 0.07 6.01<br />
2312 RB4194 53 162 hypothetical protein 2156685 2156524 11.4 5728 - 7.63 0.13 -1.20 0.06 5.15<br />
2553 RB4590 74 225 hypothetical protein 2352024 2351800 10.1 8330 - 9.62 0.00 -1.19 0.20 16.62<br />
2781 RB4951 95 288 hypothetical protein 2547498 2547785 12.1 10953 + 7.38 0.09 -1.45 0.07 4.80<br />
2782 RB4954 166 501 secreted protein 2547861 2548361 10.2 18478 + 6.60 0.13 -1.29 0.14 10.58<br />
2789 RB4966 84 255 hypothetical protein 2552311 2552057 11.3 10016 - 9.62 0.01 -1.19 0.20 16.55<br />
3045 RB5409 97 294 hypothetical protein 2794195 2794488 12.7 10898 + 8.44 0.13 -1.25 0.02 1.48<br />
3049 RB5415 62 189 hypothetical protein 2798737 2798925 12.3 7098 + 6.83 0.15 -1.70 0.05 2.72<br />
3239 RB5745 130 393 hypothetical protein 3001234 3000842 10.7 13522 - 7.49 0.19 -2.08 0.12 5.91<br />
4015 RB7116 59 180 hypothetical protein 3779586 3779765 11.7 6458 + 7.16 0.21 -1.46 0.08 5.57<br />
4314 RB7646 62 189 hypothetical protein 4111735 4111923 10.5 6950 + 7.17 0.16 -1.79 0.10 5.57<br />
4568 RB8098 71 216 hypothetical protein 4337334 4337119 10.8 7950 - 9.50 0.05 -1.05 0.15 13.86<br />
4749 RB8457 113 342 hypothetical protein 4518458 4518117 11.5 12525 - 7.35 0.16 -1.11 0.12 10.35<br />
4861 RB8669 37 114 hypothetical protein 4614557 4614670 11.5 4395 + 9.63 0.00 -1.20 0.19 15.88<br />
5885 RB10579 101 306 secreted protein 5739254 5738949 12.4 11507 - 6.90 0.21 -1.23 0.09 7.39<br />
7139 RB12815 43 132 conserved hypothetical protein 6914656 6914525 10.8 4782 - 6.89 0.20 -1.46 0.09 6.45<br />
7141 RB12818 163 492 conserved hypothetical protein 6915989 6915498 11.7 18010 - 7.28 0.19 -1.40 0.07 4.66<br />
7153 RB12837 65 198 conserved hypothetical protein 6921732 6921929 9.8 7318 + 7.81 0.19 -1.12 0.07 5.93<br />
300 minID Locus AA Nuc Product Gene EC Start Stop IEP MW(kDa) Strand Intensity Stdev Ratio Stdev %<br />
6656 RB11945 556 1671 aldehyde dehydrogenase family protein 6434111 6432441 10.9 59626 - 6.33 0.14 -1.32 0.29 21.78<br />
6865 RB12327 686 2061 TGF-beta receptor, type I/II extracellular region 6633918 6631858 4.5 75645 - 6.26 0.12 -1.42 0.19 13.19<br />
4459 RB7894 398 1197 translation elongation factor EF-Tu 4217484 4218680 5.2 43184 + 7.17 0.32 -1.51 0.33 21.65<br />
3525 RB6271 325 978 Tyrosine recombinase xerD xerD 3294814 3295791 10 36623 + 6.23 0.15 -1.13 0.25 22.21<br />
5375 RB9629 330 993 acyl-lipid delta-9 desaturase ADS2 1.14.19.- 5184385 5183393 6.5 37640 - 6.04 0.22 -1.12 0.10 8.90<br />
642 RB1205 456 1371 choline sulfatase betC 3.1.6.6 615037 613667 6.6 51848 - 6.31 0.08 -1.29 0.13 10.47<br />
597 RB1134 537 1614 NADH dehydrogenase subunit F 585258 583645 9.4 57512 - 6.36 0.21 -1.72 0.31 18.20<br />
982 RB1866 335 1008 ABC transporter, ATP-binding protein 961124 962131 4.9 36905 + 6.17 0.10 -1.11 0.06 5.46<br />
5016 RB8937 560 1683 NADH oxidase 1.6.99.3 4763877 4762195 5.3 59977 - 6.22 0.08 -1.18 0.20 17.24<br />
594 RB1129 895 2688 conserved hypothetical protein 583271 580584 5.8 99242 - 6.13 0.19 -1.28 0.28 21.59<br />
516 RB951 234 705 protein containing DUF1596 502459 501755 12.3 25583 - 6.27 0.13 -1.13 0.11 9.49<br />
1950 RB3575 152 459 membrane protein 1858779 1859237 10.1 17195 + 6.06 0.07 -1.04 0.09 8.84<br />
2312 RB4194 53 162 hypothetical protein 2156685 2156524 11.4 5728 - 7.13 0.31 -1.10 0.26 23.81<br />
2553 RB4590 74 225 hypothetical protein 2352024 2351800 10.1 8330 - 9.26 0.31 -1.91 0.18 9.45
2596 RB4657 123 372 hypothetical protein 2381865 2382236 12.2 13398 + 6.22 0.12 -1.41 0.18 12.70<br />
2789 RB4966 84 255 hypothetical protein 2552311 2552057 11.3 10016 - 9.02 0.29 -1.40 0.19 13.40<br />
3239 RB5745 130 393 hypothetical protein 3001234 3000842 10.7 13522 - 7.03 0.28 -1.21 0.21 17.25<br />
3836 RB6789 180 543 secreted protein 3608487 3607945 5 20191 - 6.49 0.11 -1.94 0.10 4.91<br />
4315 RB7647 73 222 hypothetical protein 4111931 4112152 7.4 8269 + 8.52 0.24 -1.36 0.11 8.34<br />
4568 RB8098 71 216 hypothetical protein 4337334 4337119 10.8 7950 - 8.88 0.49 -1.50 0.26 17.16<br />
4749 RB8457 113 342 hypothetical protein 4518458 4518117 11.5 12525 - 6.97 0.28 -1.12 0.26 22.83<br />
4952 RB8833 317 954 Zinc transporter ZIP 4696475 4697428 6.8 33766 + 6.25 0.04 -1.25 0.27 21.90<br />
5167 RB9243 326 981 hypothetical protein 4943853 4942873 6.1 36695 - 6.22 0.12 -1.10 0.05 4.74<br />
5214 RB9343 59 180 hypothetical protein 5003789 5003968 11.4 6521 + 6.30 0.11 -1.57 0.05 3.30<br />
5225 RB9354 183 552 hypothetical protein 5011855 5012406 10.5 20002 + 6.23 0.24 -1.03 0.11 11.19<br />
5236 RB9376 1827 5484 protein containing cadherin domain 5029102 5023619 3.8 197136 - 6.12 0.23 -1.06 0.04 4.11<br />
5253 RB9410 138 417 hypothetical protein 5048582 5048166 10 15146 - 6.18 0.21 -1.29 0.19 14.77<br />
5258 RB9417 103 312 hypothetical protein 5050621 5050932 10.5 11390 + 6.07 0.19 -1.01 0.19 19.26<br />
5283 RB9469 409 1230 membrane protein 5081396 5082625 4.3 44608 + 6.31 0.11 -1.50 0.10 6.45<br />
5304 RB9501 169 510 conserved hypothetical protein 5102922 5103431 5.6 18781 + 6.25 0.19 -1.23 0.12 9.54<br />
5318 RB9529 113 342 hypothetical protein 5117956 5118297 9.3 13082 + 6.25 0.16 -1.17 0.18 15.51<br />
5503 RB9872 67 204 hypothetical protein 5316692 5316895 5.4 6912 + 6.14 0.21 -1.13 0.21 18.39<br />
5735 RB10286 48 147 hypothetical protein 5558157 5558011 11.9 5389 - 8.17 0.47 -1.05 0.06 6.04<br />
6258 RB11245 53 162 hypothetical protein 6099126 6098965 6.3 6073 - 6.23 0.14 -1.08 0.13 12.23<br />
6343 RB11392 148 447 conserved hypothetical protein 6177208 6177654 5 16382 + 6.30 0.20 -1.25 0.14 11.48<br />
6371 RB11439 93 282 conserved hypothetical protein 6197993 6198274 10.6 10906 + 7.54 0.28 -1.51 0.17 10.95<br />
6385 RB11450 60 183 conserved hypothetical protein 6200046 6200228 11.8 6517 + 8.24 0.25 -1.52 0.11 7.51<br />
6569 RB11766 129 390 conserved hypothetical protein 6334355 6334744 10.4 14762 + 6.20 0.16 -1.06 0.04 3.64<br />
6781 RB12193 36 111 conserved hypothetical protein 6575977 6575867 7.5 3719 - 6.29 0.14 -1.47 0.27 18.06<br />
6803 RB12223 253 762 conserved hypothetical protein 6587469 6588230 10.1 27335 + 6.26 0.05 -1.07 0.19 18.27<br />
6822 RB12260 81 246 hypothetical protein-signal peptide prediction 6600777 6600532 11.9 9294 - 6.10 0.17 -1.02 0.08 7.91<br />
6857 RB12318 55 168 conserved hypothetical protein 6625565 6625398 11 6095 - 6.27 0.30 -1.43 0.15 10.66<br />
6941 RB12454 199 600 conserved hypothetical protein 6703647 6703048 10.3 22347 - 6.08 0.21 -1.04 0.23 22.60<br />
6958 RB12485 78 237 conserved hypothetical protein 6720957 6721193 7.6 8458 + 6.34 0.15 -1.38 0.15 10.99<br />
7009 RB12581 174 525 conserved hypothetical protein 6780597 6780073 9.9 19433 - 6.15 0.10 -1.12 0.25 22.39
Salinity Induced<br />
10min ID Locus START STOP EC AA Nuc Product Gene EC IEP Strand Ratio Stdev %<br />
6831 RB12276 6605112 6605444 110 333 conserved hypothetical protein 4.7 + 1.41 0.42 30<br />
3911 RB6930 3680317 3679076 1.4.1.3 413 1242 glutamate dehydrogenase 1.4.1.3 5.9 - 1.02 0.32 31<br />
7362 RB13241 7103064 7102561 167 504 RNA polymerase ECF-type sigma factor 8.8 - 2.41 0.85 35<br />
6578 RB11803 6362243 6363121 292 879 ISxac3 transposase 10 + 1.10 0.34 31<br />
6898 RB12388 6667206 6665905 433 1302 ISXo8 transposase 9.4 - 1.13 0.11 10<br />
1197 RB2268 1191389 1190541 1.8.4.6 282 849 peptide methionine sulfoxide reductase msrB 1.8.4.6 9.9 - 1.41 0.25 18<br />
6414 RB11488 6213893 6214690 265 798 oxidoreductase, short-chain dehydrogenase/reductase family 5.1 + 1.21 0.28 23<br />
5109 RB9132 4886727 4888004 425 1278 conserved hypothetical protein 5.5 + 1.07 0.20 19<br />
362 RB663 357608 357369 79 240 hypothetical protein 11.4 - 1.24 0.36 29<br />
748 RB1395 712097 713056 319 960 secreted protein 5.5 + 1.19 0.32 26<br />
1028 RB1961 1033791 1034144 117 354 hypothetical protein 11.9 + 1.11 0.13 12<br />
1454 RB2682 1393781 1393530 83 252 hypothetical protein 7.4 - 1.03 0.28 27<br />
1553 RB2875 1497313 1497534 73 222 hypothetical protein 9.9 + 2.42 0.58 24<br />
1711 RB3159 1629337 1629116 73 222 protein containing DUF1590 12.1 - 1.09 0.33 30<br />
2465 RB4438 2278247 2278729 160 483 Pyridoxamine 5'-phosphate oxidase- 4.4 + 1.43 0.45 31<br />
2493 RB4482 2297240 2296905 111 336 hypothetical protein 12 - 1.04 0.23 22<br />
3009 RB5347 2764290 2764442 50 153 hypothetical protein 11.9 + 1.30 0.37 29<br />
3305 RB5869 3078569 3077796 257 774 conserved hypothetical protein 9.8 - 3.09 0.34 11<br />
3319 RB5887 3087343 3086465 292 879 integrase 9.9 - 1.49 0.42 28<br />
4157 RB7389 3953590 3954717 375 1128 Putative transposase 9.9 + 1.33 0.21 16<br />
5217 RB9346 5008335 5008550 71 216 hypothetical protein 10.5 + 1.25 0.40 32<br />
5218 RB9347 5008600 5008394 68 207 hypothetical protein 9.9 - 1.23 0.42 34<br />
5720 RB10256 5543753 5544559 268 807 hypothetical protein 4.1 + 1.09 0.31 29<br />
5723 RB10263 5546338 5546162 58 177 hypothetical protein 9.6 - 1.31 0.35 27<br />
5764 RB10341 5593416 5593601 61 186 hypothetical protein 4.5 + 1.16 0.37 32<br />
5968 RB10728 5831649 5832047 132 399 secreted protein 9.9 + 2.07 0.49 24<br />
6424 RB11504 6221285 6221067 72 219 conserved hypothetical protein 10.7 - 1.97 0.41 21<br />
6760 RB12150 6556901 6557062 53 162 conserved hypothetical protein 4.1 + 1.04 0.16 16<br />
6815 RB12243 6596256 6596423 55 168 conserved hypothetical protein 10.5 + 1.11 0.31 28<br />
6915 RB12416 6679578 6680456 292 879 secreted protein 6 + 1.42 0.39 27<br />
7130 RB12803 6909112 6908939 57 174 conserved hypothetical protein 10.2 - 1.23 0.26 21<br />
7151 RB12833 6921305 6921634 109 330 conserved hypothetical protein 12 + 1.69 0.36 21<br />
20min ID Locus AA Nuc Product Gene EC IEP Strand Ratio Stdev %<br />
6831 RB12276 6605112 6605444 110 333 conserved hypothetical protein 4.7 + 1.11 0.20 18<br />
7371 RB13260 7108384 7109364 1.-.-.- 326 981 Zinc-containing alcohol dehydrogenase superfamily 1.-.-.- 4.6 + 1.08 0.12 11<br />
5783 RB10378 5610497 5610063 144 435 Thioredoxin trxA 4.6 - 1.18 0.15 13<br />
6884 RB12362 6653786 6652962 274 825 bacterioferritin comigratory protein 5.8 - 1.54 0.09 6<br />
7362 RB13241 7103064 7102561 167 504 RNA polymerase ECF-type sigma factor 8.8 - 1.16 0.28 24<br />
1079 RB2059 1084172 1082949 407 1224 NAD-dependent epimerase/dehydratase 10.5 - 1.07 0.20 19<br />
7369 RB13257 7105590 7106450 3.7.1.- 286 861 2-hydroxy-6-ketonona-2,4-dienedioic acid hydrolase bphD 3.7.1.- 4.6 + 1.09 0.15 13<br />
5721 RB10258 5544584 5545453 3.1.3.2 289 870 Survival protein SurE 3.1.3.2 5.3 + 2.66 0.26 10<br />
2494 RB4484 2297267 2298565 3.-.-.- 432 1299 Peptidase M20 3.-.-.- 6 + 1.70 0.14 8<br />
1813 RB3342 1716496 1715684 270 813 LmbE-like protein 5.4 - 1.20 0.20 17<br />
2453 RB4419 2272912 2273448 178 537 MgtC/SapB transporter 6.2 + 1.56 0.29 18<br />
6720 RB12068 6512255 6510594 553 1662 serine/threonine protein kinase 8.5 - 1.12 0.03 3<br />
5931 RB10665 5789912 5790514 200 603 hypothetical protein 5.3 + 1.61 0.10 6<br />
5753 RB10324 5580658 5580846 62 189 hypothetical protein 9 + 1.11 0.08 7<br />
7092 RB12737 6863917 6864114 65 198 conserved hypothetical protein 9.1 + 1.19 0.14 12<br />
2679 RB4792 2454360 2454998 212 639 secreted protein 9.4 + 1.09 0.18 16<br />
3305 RB5869 3078569 3077796 257 774 conserved hypothetical protein 9.8 - 1.49 0.18 12<br />
1553 RB2875 1497313 1497534 73 222 hypothetical protein 9.9 + 1.73 0.15 9<br />
1728 RB3196 1650093 1650422 109 330 hypothetical protein 10.1 + 1.22 0.16 13<br />
6097 RB10954 5940749 5940318 143 432 hypothetical protein 10.4 - 1.15 0.16 14<br />
6066 RB10895 5916348 5917151 267 804 hypothetical protein 10.5 + 1.19 0.07 5<br />
6424 RB11504 6221285 6221067 72 219 conserved hypothetical protein 10.7 - 1.10 0.11 10<br />
3326 RB5899 3092014 3091748 88 267 hypothetical protein 10.9 - 1.67 0.04 2<br />
5766 RB10343 5593898 5594059 53 162 hypothetical protein 11.3 + 1.73 0.27 16<br />
6101 RB10960 5941602 5942021 139 420 conserved hypothetical protein, membrane 11.4 + 1.05 0.15 14<br />
5815 RB10440 5655693 5655842 49 150 hypothetical protein 11.5 + 1.48 0.03 2<br />
4982 RB8884 4727666 4727878 70 213 hypothetical protein 11.8 + 1.04 0.05 5<br />
7113 RB12770 6884806 6884306 166 501 conserved hypothetical protein 11.9 - 1.22 0.27 22<br />
2493 RB4482 2297240 2296905 111 336 hypothetical protein 12 - 1.44 0.02 1<br />
40min ID Locus AA Nuc Product Gene EC IEP Strand Ratio Stdev %<br />
6831 RB12276 6605112 6605444 110 333 conserved hypothetical protein 4.7 + 2.02 0.22 11<br />
5783 RB10378 5610497 5610063 144 435 Thioredoxin trxA 4.6 - 1.49 0.33 22<br />
6884 RB12362 6653786 6652962 274 825 bacterioferritin comigratory protein 5.8 - 1.07 0.09 9<br />
4626 RB8222 4396604 4398475 2.3.1.31 623 1872 Homoserine O-acetyltransferase metX 2.3.1.31 4.5 + 1.13 0.24 21<br />
6891 RB12379 6662808 6662086 240 723 riboflavin synthase, alpha subunit 4.6 - 1.09 0.12 11<br />
364 RB668 357993 358883 296 891 lysophospholipase 5 + 1.43 0.22 15<br />
2463 RB4433 2277208 2277696 162 489 Ferritin and Dps 4.3 + 1.48 0.33 22<br />
2213 RB3999 2064741 2063431 436 1311 site-specific recombinase, 10.4 - 1.17 0.06 5<br />
403 RB754 403211 404305 364 1095 Polysaccharide export protein 4.2 + 1.04 0.25 24<br />
6833 RB12279 6606558 6605545 337 1014 MscS Mechanosensitive ion channel 4.5 - 2.22 0.75 34<br />
5719 RB10255 5542656 5543669 337 1014 MscS Mechanosensitive ion channel 5.4 + 2.47 0.26 10<br />
6425 RB11505 6221829 6221230 199 600 conserved hypothetical protein, secreted 7.5 - 2.30 0.16 7<br />
5782 RB10374 5609916 5609188 3.4.23.36 242 729 Lipoprotein signal peptidase lspA 3.4.23.36 7.5 - 1.38 0.16 12<br />
3325 RB5897 3091814 3090681 377 1134 secreted protein 8.6 - 1.14 0.75 66<br />
2462 RB4432 2276333 2277139 1.1.1.- 268 807 glucose 1-dehydrogenase or 3-oxoacyl-[acyl-carrier-protein] reductase 1.1.1.- 4.7 + 1.31 0.04 3<br />
2437 RB4394 2261372 2261938 3.2.-.- 188 567 protease I pfpI 3.2.-.- 4.4 + 1.12 0.09 8<br />
6104 RB10967 5944040 5943015 341 1026 quinone oxidoreductase-like protein 4.5 - 1.53 0.18 12<br />
6215 RB11173 6063194 6063586 130 393 conserved hypothetical protein, secreted 4.7 + 1.65 0.34 20<br />
5762 RB10338 5591839 5590379 486 1461 TPR domain protein 5 - 2.52 2.70 107<br />
5721 RB10258 5544584 5545453 3.1.3.2 289 870 Survival protein SurE 3.1.3.2 5.3 + 4.50 0.52 11<br />
2494 RB4484 2297267 2298565 3.-.-.- 432 1299 Peptidase M20 3.-.-.- 6 + 2.50 0.13 5<br />
2495 RB4485 2298652 2299770 6.3.2.2 372 1119 Glutamate--cysteine ligase, GCS2 6.3.2.2 4.7 + 1.89 0.84 45<br />
2453 RB4419 2272912 2273448 178 537 MgtC/SapB transporter 6.2 + 3.06 0.15 5<br />
2505 RB4504 2304293 2304838 181 546 membrane protein containing DUF421 7.5 + 1.14 0.17 15<br />
6720 RB12068 6512255 6510594 553 1662 serine/threonine protein kinase 8.5 - 3.72 0.11 3<br />
2488 RB4474 2295048 2295554 168 507 CsbD-like 8.8 + 1.59 0.28 17<br />
2454 RB4420 2274093 2273473 206 621 DedA family protein 9.3 - 1.79 0.16 9<br />
6266 RB11260 6107202 6106837 121 366 dnaK suppressor protein, 5.2 - 1.11 0.24 22<br />
7296 RB13118 7037043 7037675 210 633 two-component system response regulator 8.8 + 2.01 0.23 11<br />
1449 RB2673 1390600 1389086 2.7.1.37 504 1515 serine/threonine protein kinase spk1 2.7.1.37 9.2 - 1.06 0.15 14<br />
2503 RB4500 2303645 2303908 87 264 conserved hypothetical protein, secreted 3.5 + 1.19 0.30 25<br />
4705 RB8367 4463905 4464057 50 153 hypothetical protein 3.7 + 1.01 0.06 6<br />
4586 RB8138 4351960 4352187 75 228 hypothetical protein 4.1 + 2.08 0.36 17<br />
4587 RB8140 4353931 4352189 580 1743 hypothetical protein 4.1 - 1.16 0.20 17<br />
5720 RB10256 5543753 5544559 268 807 hypothetical protein 4.1 + 2.51 0.65 26<br />
1053 RB2011 1059411 1059692 93 282 hypothetical protein 4.4 + 1.04 0.13 12<br />
2465 RB4438 2278247 2278729 160 483 Pyridoxamine 5'-phosphate oxidase- 4.4 + 1.95 0.16 8<br />
6848 RB12303 6618759 6616882 625 1878 serine/threonine protein kinase related protein 4.5 - 2.05 2.28 111<br />
2485 RB4468 2294104 2293616 162 489 hypothetical protein 4.6 - 1.25 0.07 6<br />
2407 RB4347 2235735 2236205 156 471 conserved hypothetical protein 4.7 + 1.44 0.29 20<br />
6098 RB10956 5940336 5940689 117 354 hypothetical protein 4.8 + 1.36 0.20 14<br />
2461 RB4429 2275996 2276265 89 270 conserved hypothetical protein 4.9 + 1.16 0.13 11<br />
2486 RB4470 2294676 2294167 169 510 secreted protein 4.9 - 1.25 0.23 19<br />
5931 RB10665 5789912 5790514 200 603 hypothetical protein 5.3 + 1.48 0.34 23<br />
2497 RB4489 2301387 2301905 172 519 hypothetical protein 5.4 + 2.36 0.05 2<br />
6100 RB10958 5941015 5941491 158 477 hypothetical protein 5.4 + 1.41 0.19 14<br />
748 RB1395 712097 713056 319 960 secreted protein 5.5 + 1.36 0.14 10<br />
1452 RB2679 1391557 1392078 173 522 hypothetical protein 5.5 + 2.33 0.15 6<br />
6099 RB10957 5940708 5941007 99 300 conserved hypothetical protein 5.6 + 1.06 0.19 18<br />
2492 RB4480 2296646 2296915 89 270 hypothetical protein 6.1 + 1.66 0.17 10<br />
1453 RB2680 1393566 1392163 467 1404 conserved hypothetical protein, membrane 6.4 - 1.07 0.05 5<br />
868 RB1617 834021 833623 132 399 hypothetical protein 6.8 - 1.55 0.17 11<br />
2491 RB4478 2296122 2296640 172 519 membrane protein 7.3 + 1.64 0.19 11<br />
1454 RB2682 1393781 1393530 83 252 hypothetical protein 7.4 - 1.55 0.31 20
4882 RB8703 4630992 4631468 158 477 hypothetical protein 7.4 + 1.03 0.11 11<br />
3395 RB6018 3154134 3153562 190 573 hypothetical protein 8.7 - 1.22 0.05 4<br />
3585 RB6375 3349681 3350574 297 894 hypothetical protein 8.7 + 1.41 0.23 16<br />
2509 RB4510 2307657 2307508 49 150 hypothetical protein 9 - 2.56 0.22 9<br />
7092 RB12737 6863917 6864114 65 198 conserved hypothetical protein 9.1 + 2.09 0.08 4<br />
2501 RB4498 2303522 2303250 90 273 hypothetical protein 9.4 - 1.58 0.80 51<br />
3900 RB6910 3671484 3671356 42 129 hypothetical protein 9.5 - 1.46 0.81 56<br />
946 RB1787 925904 925686 72 219 hypothetical protein 9.6 - 1.04 0.05 5<br />
6064 RB10893 5914894 5915067 57 174 hypothetical protein 9.7 + 1.68 0.35 21<br />
3909 RB6925 3678461 3677976 161 486 hypothetical protein 9.8 - 1.67 0.06 4<br />
7293 RB13115 7036614 7036369 81 246 conserved hypothetical protein 9.8 - 2.87 0.16 6<br />
1553 RB2875 1497313 1497534 73 222 hypothetical protein 9.9 + 1.62 0.26 16<br />
2498 RB4492 2302138 2301833 101 306 hypothetical protein 9.9 - 1.41 0.14 10<br />
6718 RB12066 6510147 6510554 135 408 conserved hypothetical protein 10 + 1.22 0.15 12<br />
365 RB670 359315 358905 136 411 hypothetical protein 10.1 - 2.17 0.22 10<br />
1728 RB3196 1650093 1650422 109 330 hypothetical protein 10.1 + 1.49 0.17 12<br />
6096 RB10951 5940232 5939927 101 306 secreted protein 10.2 - 1.64 0.18 11<br />
6157 RB11070 6000321 6000479 52 159 hypothetical protein 10.2 + 1.71 0.32 19<br />
6097 RB10954 5940749 5940318 143 432 hypothetical protein 10.4 - 3.01 0.12 4<br />
1207 RB2283 1197719 1197102 205 618 protein containing DUF1590 10.5 - 1.03 0.08 8<br />
2440 RB4397 2263976 2264143 55 168 protein containing DUF1560 10.5 + 1.41 0.41 29<br />
6066 RB10895 5916348 5917151 267 804 hypothetical protein 10.5 + 2.41 0.12 5<br />
1911 RB3505 1816597 1816704 35 108 secreted protein containing DUF1586 10.6 + 1.72 0.24 14<br />
2457 RB4425 2275100 2275483 127 384 hypothetical protein 10.6 + 1.04 0.13 13<br />
2490 RB4477 2295940 2296149 69 210 hypothetical protein 10.6 + 1.07 0.03 2<br />
301 RB521 286399 286208 63 192 hypothetical protein 10.7 - 2.64 0.10 4<br />
366 RB671 359218 359586 122 369 hypothetical protein 10.7 + 2.60 0.15 6<br />
2502 RB4499 2303764 2303519 81 246 hypothetical protein 10.7 - 2.15 0.14 6<br />
6424 RB11504 6221285 6221067 72 219 conserved hypothetical protein 10.7 - 3.37 0.07 2<br />
5068 RB9057 4834849 4834562 95 288 hypothetical protein 10.9 - 1.47 0.12 8<br />
5901 RB10607 5759926 5759537 129 390 hypothetical protein 11 - 1.74 0.23 13<br />
1487 RB2741 1430170 1429994 58 177 hypothetical protein 11.2 - 1.31 0.15 11<br />
1656 RB3052 1577799 1577692 35 108 hypothetical protein 11.2 - 1.48 0.20 13<br />
5766 RB10343 5593898 5594059 53 162 hypothetical protein 11.3 + 1.79 0.09 5<br />
6048 RB10868 5900330 5899920 136 411 hypothetical protein 11.3 - 1.06 0.27 25<br />
2489 RB4476 2295557 2295943 128 387 hypothetical protein 11.4 + 1.57 0.38 24<br />
6220 RB11181 6067002 6067109 35 108 secreted protein containing DUF1586 11.4 + 1.50 0.22 14<br />
5815 RB10440 5655693 5655842 49 150 hypothetical protein 11.5 + 1.23 0.11 9<br />
7113 RB12770 6884806 6884306 166 501 conserved hypothetical protein 11.9 - 2.25 0.23 10<br />
2493 RB4482 2297240 2296905 111 336 hypothetical protein 12 - 1.65 0.21 13<br />
7151 RB12833 6921305 6921634 109 330 conserved hypothetical protein 12 + 1.25 0.13 10<br />
60min ID Locus AA Nuc Product Gene EC IEP Strand Ratio Stdev %<br />
6270 RB11269 6109738 6110583 281 846 CYTOCHROME C SIGNAL PEPTIDE PROTEIN 8.6 + 1.45 0.37 25<br />
6831 RB12276 6605112 6605444 110 333 conserved hypothetical protein 4.7 + 2.62 0.16 6<br />
7243 RB13025 6997019 6996438 193 582 monooxygenase 9.7 - 1.58 0.37 23<br />
5783 RB10378 5610497 5610063 144 435 Thioredoxin trxA 4.6 - 1.21 0.16 13<br />
4626 RB8222 4396604 4398475 2.3.1.31 623 1872 Homoserine O-acetyltransferase metX 2.3.1.31 4.5 + 1.73 0.32 18<br />
2927 RB5196 2678058 2675953 2.4.1.4 701 2106 trehalose synthase treS 5.4.99.16 5.6 - 1.39 0.18 13<br />
5803 RB10416 5634693 5639387 3.2.1.8 1564 4695 xylanase-like protein 3.2.1.8 4 + 1.52 0.30 20<br />
6283 RB11291 6120208 6120648 146 441 conserved hypothetical protein 4.5 + 1.02 0.26 26<br />
6723 RB12073 6513974 6513066 5.3.1.22 302 909 hydroxypyruvate isomerase protein hyi 5.3.1.22 5.6 - 1.22 0.18 15<br />
4744 RB8445 4514475 4514050 141 426 Penicillinase repressor 9.9 - 1.26 0.20 16<br />
5348 RB9581 5150921 5150535 128 387 protein containing DUF24 8.7 - 1.06 0.25 23<br />
5571 RB9999 5386828 5385983 281 846 conserved hypothetical protein 4.6 - 1.25 0.03 2<br />
5606 RB10049 5419507 5420160 217 654 RNA polymerase ECF-type sigma factor 10.1 + 1.80 0.22 12<br />
7362 RB13241 7103064 7102561 167 504 RNA polymerase ECF-type sigma factor 8.8 - 3.06 0.38 12<br />
629 RB1190 607131 608009 292 879 integrase 10 + 1.51 0.35 23<br />
2381 RB4299 2214533 2214823 96 291 Transposase IS3/IS911 9.9 + 1.81 0.49 27<br />
2382 RB4300 2214826 2215704 292 879 integrase 10 + 1.16 0.22 19<br />
2463 RB4433 2277208 2277696 162 489 Ferritin and Dps 4.3 + 1.20 0.10 8<br />
3319 RB5887 3087343 3086465 292 879 integrase 9.9 - 1.27 0.08 7<br />
3474 RB6171 3239091 3238048 347 1044 site-specific recombinase 10.7 - 1.22 0.23 19<br />
6559 RB11750 6328357 6329235 292 879 integrase 10 + 1.59 0.26 17<br />
7203 RB12940 6966246 6966536 96 291 Transposase IS3/IS911 10.1 + 1.53 0.38 25<br />
7204 RB12941 6966539 6967417 292 879 integrase 10 + 1.08 0.12 11<br />
7333 RB13189 7081765 7083066 433 1302 ISXo8 transposase 9.4 + 1.43 0.09 7<br />
5367 RB9617 5176667 5175471 2.4.1.- 398 1197 mannosyltransferase 2.4.1.- 5.4 - 1.25 0.02 2<br />
5719 RB10255 5542656 5543669 337 1014 MscS Mechanosensitive ion channel 5.4 + 2.65 0.15 6<br />
6833 RB12279 6606558 6605545 337 1014 MscS Mechanosensitive ion channel 4.5 - 2.70 0.10 4<br />
1079 RB2059 1084172 1082949 407 1224 NAD-dependent epimerase/dehydratase 10.5 - 1.56 0.34 22<br />
6425 RB11505 6221829 6221230 199 600 conserved hypothetical protein, secreted 7.5 - 2.40 0.09 4<br />
7116 RB12774 6885973 6887763 596 1791 Bacterial type II secretion system protein E 4.2 + 1.18 0.30 25<br />
7118 RB12781 6888383 6889822 479 1440 pilus assembly protein PilC 5.6 + 1.14 0.13 11<br />
6727 RB12080 6517231 6515753 2.1.1.77 492 1479 Protein-L-isoaspartate(D-aspartate) O-methyltransferase 2.1.1.77 8.9 - 1.43 0.12 8<br />
6102 RB10965 5942116 5942730 204 615 conserved hypothetical protein, membrane 4.9 + 1.74 0.38 22<br />
3555 RB6327 3321757 3322500 247 744 oxidoreductase, short chain dehydrogenase/reductase family 6.2 + 1.39 0.04 3<br />
3584 RB6374 3348288 3349616 442 1329 conserved hypothetical protein 9 + 1.16 0.15 13<br />
3648 RB6469 3419515 3420273 252 759 ABC transporter ATP-binding protein 5.6 + 1.33 0.30 22<br />
6414 RB11488 6213893 6214690 265 798 oxidoreductase, short-chain dehydrogenase/reductase family 5.1 + 2.02 0.20 10<br />
6423 RB11502 6219763 6221100 445 1338 alkyl sulfatase or beta-lactamase 5.9 + 1.04 0.09 9<br />
2800 RB4981 2559627 2560415 1.1.1.100 262 789 3-oxoacyl-[acyl-carrier-protein] reductase fabG 1.1.1.100 4.4 + 1.39 0.29 21<br />
2494 RB4484 2297267 2298565 3.-.-.- 432 1299 Peptidase M20 3.-.-.- 6 + 1.36 0.07 5<br />
5109 RB9132 4886727 4888004 425 1278 conserved hypothetical protein 5.5 + 1.11 0.24 21<br />
5721 RB10258 5544584 5545453 3.1.3.2 289 870 Survival protein SurE 3.1.3.2 5.3 + 2.77 0.17 6<br />
6215 RB11173 6063194 6063586 130 393 conserved hypothetical protein, secreted 4.7 + 1.98 0.02 1<br />
7369 RB13257 7105590 7106450 3.7.1.- 286 861 2-hydroxy-6-ketonona-2,4-dienedioic acid hydrolase bphD 3.7.1.- 4.6 + 1.91 0.13 7<br />
7371 RB13260 7108384 7109364 1.-.-.- 326 981 Zinc-containing alcohol dehydrogenase superfamily 1.-.-.- 4.6 + 1.34 0.17 13<br />
2453 RB4419 2272912 2273448 178 537 MgtC/SapB transporter 6.2 + 2.11 0.15 7<br />
2454 RB4420 2274093 2273473 206 621 DedA family protein 9.3 - 1.04 0.25 24<br />
2495 RB4485 2298652 2299770 6.3.2.2 372 1119 Glutamate--cysteine ligase, GCS2 6.3.2.2 4.7 + 1.33 0.14 11<br />
3490 RB6207 3252449 3253759 436 1311 conserved hypothetical protein, secreted 4.8 + 1.15 0.21 18<br />
4648 RB8260 4415394 4415068 108 327 protein containing DUF167 10.5 - 1.57 0.17 11<br />
6265 RB11258 6106840 6106127 237 714 conserved hypothetical protein, membrane 10.3 - 1.30 0.05 4<br />
6422 RB11498 6218361 6219701 446 1341 conserved hypothetical protein 5.4 + 1.30 0.12 9<br />
6720 RB12068 6512255 6510594 553 1662 serine/threonine protein kinase 8.5 - 2.41 0.14 6<br />
6266 RB11260 6107202 6106837 121 366 dnaK suppressor protein, 5.2 - 1.08 0.17 16<br />
4 RB5 1514 399 371 1116 secreted protein 4.1 - 3.14 0.32 10<br />
101 RB174 91448 91585 45 138 hypothetical protein 5.2 + 1.29 0.28 21<br />
300 RB520 284989 286116 375 1128 conserved hypothetical protein 4.8 + 1.29 0.35 27<br />
301 RB521 286399 286208 63 192 hypothetical protein 10.7 - 3.43 0.15 4<br />
328 RB568 312345 312112 77 234 hypothetical protein 10.2 - 1.28 0.28 22<br />
362 RB663 357608 357369 79 240 hypothetical protein 11.4 - 1.78 0.08 4<br />
365 RB670 359315 358905 136 411 hypothetical protein 10.1 - 2.40 0.19 8<br />
366 RB671 359218 359586 122 369 hypothetical protein 10.7 + 1.43 0.03 2<br />
507 RB934 494203 493076 375 1128 Putative transposase 9.9 - 1.28 0.03 2<br />
528 RB968 507840 507079 253 762 protein containing DUF1596 11.3 - 1.79 0.34 19<br />
556 RB1043 543083 543352 89 270 conserved hypothetical protein 4.7 + 1.07 0.08 7<br />
579 RB1093 567412 567152 86 261 hypothetical protein 9.8 - 1.13 0.13 11<br />
623 RB1180 604378 604656 92 279 hypothetical protein 12.6 + 1.23 0.28 23<br />
692 RB1294 657236 657051 61 186 hypothetical protein 9.8 - 1.10 0.13 12<br />
697 RB1302 659166 659762 198 597 hypothetical protein 8.8 + 1.44 0.09 6<br />
707 RB1322 671526 671633 35 108 hypothetical protein 12.8 + 1.32 0.03 2<br />
748 RB1395 712097 713056 319 960 secreted protein 5.5 + 1.47 0.17 12<br />
868 RB1617 834021 833623 132 399 hypothetical protein 6.8 - 1.58 0.21 14<br />
910 RB1716 895805 895557 82 249 protein containing DUF1589 10.1 - 1.33 0.23 17<br />
913 RB1722 897688 899643 651 1956 hypothetical protein 5.4 + 1.81 0.02 1<br />
914 RB1726 899926 899633 97 294 hypothetical protein 10.8 - 1.12 0.28 25<br />
915 RB1728 899954 900811 285 858 membrane protein containing DUF1206 9.9 + 1.74 0.35 20<br />
916 RB1729 901146 900784 120 363 hypothetical protein 7.7 - 3.11 0.24 8<br />
936 RB1767 919059 916336 907 2724 60-kDa cysteine-rich outer membrane protein precursor omcB 4.2 - 1.43 0.10 7<br />
941 RB1778 921245 921036 69 210 hypothetical protein 10.7 - 1.22 0.22 18
1151 RB2186 1153993 1152692 433 1302 ISXo8 transposase 9.4 - 1.46 0.09 6<br />
1207 RB2283 1197719 1197102 205 618 protein containing DUF1590 10.5 - 1.08 0.25 23<br />
1371 RB2525 1320284 1320054 76 231 hypothetical protein 6.1 - 1.11 0.28 25<br />
1435 RB2649 1374987 1375148 53 162 hypothetical protein 9.1 + 1.55 0.19 12<br />
1452 RB2679 1391557 1392078 173 522 hypothetical protein 5.5 + 1.63 0.16 10<br />
1516 RB2806 1458677 1458802 41 126 hypothetical protein 6.5 + 1.24 0.19 15<br />
1518 RB2809 1459745 1459065 226 681 hypothetical protein 9.1 - 1.13 0.05 5<br />
1519 RB2810 1459901 1459752 49 150 hypothetical protein 11.3 - 1.03 0.10 10<br />
1553 RB2875 1497313 1497534 73 222 hypothetical protein 9.9 + 1.43 0.08 6<br />
1656 RB3052 1577799 1577692 35 108 hypothetical protein 11.2 - 1.51 0.15 10<br />
1706 RB3153 1626911 1626681 76 231 hypothetical protein (protein containing DUF1584) 12.4 - 1.40 0.14 10<br />
1707 RB3154 1627155 1626925 76 231 hypothetical protein 12.7 - 1.02 0.17 17<br />
1722 RB3189 1646085 1646393 102 309 hypothetical protein 11.1 + 1.53 0.09 6<br />
1728 RB3196 1650093 1650422 109 330 hypothetical protein 10.1 + 2.28 0.13 6<br />
1791 RB3303 1695709 1694447 420 1263 conserved hypothetical protein 9.4 - 1.05 0.20 19<br />
1911 RB3505 1816597 1816704 35 108 secreted protein containing DUF1586 10.6 + 1.50 0.08 6<br />
1957 RB3587 1864796 1864455 113 342 hypothetical protein 11.8 - 1.84 0.23 13<br />
2189 RB3963 2052742 2052870 42 129 hypothetical protein 4.1 + 1.14 0.28 25<br />
2304 RB4176 2146928 2146638 96 291 hypothetical protein 12.4 - 1.28 0.25 20<br />
2352 RB4258 2181475 2180966 169 510 conserved hypothetical protein 10.3 - 1.15 0.02 2<br />
2407 RB4347 2235735 2236205 156 471 conserved hypothetical protein 4.7 + 1.22 0.10 8<br />
2412 RB4353 2237771 2237331 146 441 hypothetical protein 4.1 - 1.08 0.07 6<br />
2440 RB4397 2263976 2264143 55 168 protein containing DUF1560 10.5 + 2.56 0.21 8<br />
2457 RB4425 2275100 2275483 127 384 hypothetical protein 10.6 + 2.20 0.45 20<br />
2458 RB4426 2275786 2275604 60 183 hypothetical protein 6.3 - 1.31 0.20 15<br />
2465 RB4438 2278247 2278729 160 483 Pyridoxamine 5'-phosphate oxidase- 4.4 + 1.69 0.09 5<br />
2467 RB4443 2280143 2279973 56 171 hypothetical protein 10.1 - 1.45 0.17 12<br />
2475 RB4457 2285628 2285924 98 297 hypothetical protein 10 + 1.59 0.37 23<br />
2478 RB4460 2289696 2288428 422 1269 conserved hypothetical protein, membrane 4.8 - 1.03 0.23 22<br />
2493 RB4482 2297240 2296905 111 336 hypothetical protein 12 - 1.87 0.12 7<br />
2497 RB4489 2301387 2301905 172 519 hypothetical protein 5.4 + 1.33 0.15 12<br />
2509 RB4510 2307657 2307508 49 150 hypothetical protein 9 - 1.37 0.36 26<br />
2607 RB4676 2391079 2390414 221 666 hypothetical protein 10.4 - 1.37 0.04 3<br />
2654 RB4750 2435462 2436076 204 615 membrane protein 10 + 1.09 0.27 24<br />
2722 RB4859 2491989 2491429 186 561 RNA polymerase sigma factor 6.4 - 1.30 0.07 5<br />
2816 RB5001 2572354 2572665 103 312 hypothetical protein 10.3 + 1.26 0.07 6<br />
2819 RB5007 2574916 2575812 3.1.1.- 298 897 esterase 6 + 1.12 0.21 19<br />
2951 RB5238 2697775 2697996 73 222 hypothetical protein 10.6 + 2.06 0.02 1<br />
3326 RB5899 3092014 3091748 88 267 hypothetical protein 10.9 - 1.09 0.19 17<br />
3345 RB5937 3112147 3113067 306 921 hypothetical protein 11.7 + 1.02 0.09 9<br />
3346 RB5938 3113484 3112372 370 1113 hypothetical protein 5.6 - 1.77 0.27 15<br />
3449 RB6131 3223482 3223790 102 309 hypothetical protein 12.1 + 1.64 0.25 15<br />
3457 RB6144 3228305 3228189 38 117 hypothetical protein 11.1 - 1.83 0.29 16<br />
3585 RB6375 3349681 3350574 297 894 hypothetical protein 8.7 + 1.61 0.11 7<br />
3739 RB6627 3522169 3522699 176 531 hypothetical protein 6 + 1.21 0.11 9<br />
3770 RB6684 3554034 3553717 105 318 hypothetical protein 11.6 - 1.27 0.07 6<br />
3900 RB6910 3671484 3671356 42 129 hypothetical protein 9.5 - 2.47 0.07 3<br />
3909 RB6925 3678461 3677976 161 486 hypothetical protein 9.8 - 1.28 0.02 2<br />
3969 RB7039 3743573 3742965 202 609 hypothetical protein 7.9 - 2.01 0.47 23<br />
4077 RB7221 3832041 3831646 131 396 hypothetical protein 10.4 - 1.07 0.16 15<br />
4109 RB7279 3870019 3870309 96 291 hypothetical protein 6 + 1.02 0.21 21<br />
4135 RB7318 3895264 3894884 126 381 hypothetical protein 7.3 - 1.04 0.17 16<br />
4157 RB7389 3953590 3954717 375 1128 Putative transposase 9.9 + 1.18 0.22 19<br />
4235 RB7523 4047925 4048074 49 150 hypothetical protein 12.4 + 2.73 0.03 1<br />
4380 RB7752 4153019 4152765 84 255 hypothetical protein 9.8 - 1.20 0.27 22<br />
4465 RB7901 4221613 4221293 106 321 hypothetical protein 11.8 - 1.55 0.13 8<br />
4499 RB7965 4258303 4258464 53 162 hypothetical protein 7.4 + 1.20 0.05 4<br />
4583 RB8132 4349972 4350505 177 534 hypothetical protein 10.8 + 1.81 0.20 11<br />
4586 RB8138 4351960 4352187 75 228 hypothetical protein 4.1 + 1.73 0.38 22<br />
4590 RB8146 4355122 4354667 151 456 hypothetical protein 4.3 - 1.71 0.12 7<br />
4705 RB8367 4463905 4464057 50 153 hypothetical protein 3.7 + 1.44 0.26 18<br />
4852 RB8641 4605970 4606248 92 279 hypothetical protein 7.5 + 2.61 0.55 21<br />
4991 RB8898 4737468 4738433 321 966 acetyltransferase 4.5 + 1.65 0.27 16<br />
4994 RB8902 4740474 4740226 82 249 hypothetical protein 11.2 - 1.10 0.18 16<br />
5038 RB8987 4790856 4790710 48 147 hypothetical protein 9.1 - 1.13 0.15 13<br />
5074 RB9070 4840267 4841496 409 1230 secreted protein containing DUF1559 8.6 + 2.40 0.08 3<br />
5137 RB9191 4914516 4914653 45 138 secreted protein containing DUF1586 9.8 + 1.31 0.05 4<br />
5217 RB9346 5008335 5008550 71 216 hypothetical protein 10.5 + 1.87 0.18 10<br />
5347 RB9578 5150392 5150592 66 201 hypothetical protein 5.4 + 1.41 0.05 4<br />
5377 RB9635 5187031 5185973 352 1059 hypothetical protein 5.9 - 1.34 0.36 27<br />
5455 RB9786 5270645 5270205 146 441 hypothetical protein 12 - 1.25 0.19 15<br />
5473 RB9811 5284365 5284673 102 309 secreted protein 11.7 + 2.79 0.37 13<br />
5524 RB9907 5338185 5339486 433 1302 ISXo8 transposase 9.4 + 1.95 0.42 22<br />
5552 RB9959 5366417 5366220 65 198 hypothetical protein 11.9 - 1.32 0.29 22<br />
5723 RB10263 5546338 5546162 58 177 hypothetical protein 9.6 - 1.02 0.23 22<br />
5753 RB10324 5580658 5580846 62 189 hypothetical protein 9 + 1.24 0.20 16<br />
5764 RB10341 5593416 5593601 61 186 hypothetical protein 4.5 + 1.71 0.11 6<br />
5766 RB10343 5593898 5594059 53 162 hypothetical protein 11.3 + 2.10 0.15 7<br />
5815 RB10440 5655693 5655842 49 150 hypothetical protein 11.5 + 1.81 0.05 3<br />
5834 RB10473 5679433 5681868 811 2436 conserved hypothetical protein 5.4 + 1.62 0.42 26<br />
5876 RB10560 5731867 5729063 934 2805 hypothetical protein 4.6 - 1.09 0.09 8<br />
5901 RB10607 5759926 5759537 129 390 hypothetical protein 11 - 1.36 0.08 6<br />
5931 RB10665 5789912 5790514 200 603 hypothetical protein 5.3 + 2.55 0.29 11<br />
6064 RB10893 5914894 5915067 57 174 hypothetical protein 9.7 + 1.43 0.30 21<br />
6066 RB10895 5916348 5917151 267 804 hypothetical protein 10.5 + 2.03 0.11 6<br />
6067 RB10896 5917040 5916555 161 486 secreted protein 10 - 1.33 0.03 2<br />
6097 RB10954 5940749 5940318 143 432 hypothetical protein 10.4 - 1.15 0.04 3<br />
6101 RB10960 5941602 5942021 139 420 conserved hypothetical protein, membrane 11.4 + 1.15 0.11 9<br />
6124 RB11000 5964054 5963380 224 675 conserved hypothetical protein, membrane 8.8 - 2.06 0.03 1<br />
6157 RB11070 6000321 6000479 52 159 hypothetical protein 10.2 + 1.28 0.07 6<br />
6178 RB11110 6022600 6023529 309 930 Rhodopirellula transposase 5.8 + 1.30 0.10 7<br />
6220 RB11181 6067002 6067109 35 108 secreted protein containing DUF1586 11.4 + 1.29 0.03 2<br />
6236 RB11209 6080603 6080914 103 312 hypothetical protein 9.5 + 1.86 0.41 22<br />
6237 RB11210 6080955 6080653 100 303 hypothetical protein 9.9 - 1.49 0.04 2<br />
6269 RB11268 6109768 6109475 97 294 hypothetical protein 10.8 - 1.41 0.22 16<br />
6321 RB11348 6155692 6155237 151 456 conserved hypothetical protein 3.4 - 1.02 0.03 3<br />
6424 RB11504 6221285 6221067 72 219 conserved hypothetical protein 10.7 - 3.38 0.08 2<br />
6430 RB11515 6224420 6224644 74 225 conserved hypothetical protein 11.7 + 1.79 0.18 10<br />
6472 RB11603 6256722 6256835 37 114 conserved hypothetical protein 9 + 1.48 0.29 19<br />
6571 RB11768 6334808 6334939 43 132 conserved hypothetical protein 9.4 + 1.50 0.33 22<br />
6606 RB11855 6383765 6383460 101 306 conserved hypothetical protein 12.5 - 1.30 0.09 7<br />
6673 RB11977 6455958 6456548 196 591 conserved hypothetical protein 9.5 + 1.49 0.14 10<br />
6691 RB12009 6479495 6477951 514 1545 conserved hypothetical protein 4.8 - 1.17 0.15 12<br />
6721 RB12071 6512255 6512866 203 612 conserved hypothetical protein 10.7 + 1.15 0.19 17<br />
6760 RB12150 6556901 6557062 53 162 conserved hypothetical protein 4.1 + 1.83 0.10 5<br />
6810 RB12236 6590935 6591546 203 612 conserved hypothetical protein, membrane 4.8 + 1.16 0.18 16<br />
6812 RB12239 6593334 6594635 433 1302 ISXo8 transposase 9.4 + 2.57 0.02 1<br />
6815 RB12243 6596256 6596423 55 168 conserved hypothetical protein 10.5 + 3.56 0.01 0<br />
6817 RB12247 6597048 6596824 74 225 conserved hypothetical protein 6.3 - 2.33 0.32 14<br />
6844 RB12295 6614190 6614450 86 261 conserved hypothetical protein 9.1 + 1.32 0.24 18<br />
6861 RB12323 6627001 6627747 248 747 conserved hypothetical protein 4.4 + 1.32 0.03 2<br />
6862 RB12324 6627901 6627713 62 189 conserved hypothetical protein 12.3 - 1.30 0.03 2<br />
6898 RB12388 6667206 6665905 433 1302 ISXo8 transposase 9.4 - 1.92 0.18 9<br />
6951 RB12473 6712524 6712706 60 183 conserved hypothetical protein 7.3 + 1.47 0.13 9<br />
7092 RB12737 6863917 6864114 65 198 conserved hypothetical protein 9.1 + 2.02 0.07 3<br />
7099 RB12747 6870758 6870919 53 162 conserved hypothetical protein 11.8 + 1.13 0.18 16<br />
7113 RB12770 6884806 6884306 166 501 conserved hypothetical protein 11.9 - 2.28 0.32 14<br />
7119 RB12782 6889986 6889819 55 168 conserved hypothetical protein 10.9 - 2.17 0.05 2<br />
7120 RB12783 6889970 6891277 435 1308 conserved hypothetical protein 4.5 + 1.45 0.28 19<br />
7130 RB12803 6909112 6908939 57 174 conserved hypothetical protein 10.2 - 1.12 0.14 12<br />
7148 RB12829 6919813 6920292 159 480 conserved hypothetical protein 9.5 + 1.33 0.04 3<br />
7151 RB12833 6921305 6921634 109 330 conserved hypothetical protein 12 + 1.14 0.07 6<br />
7202 RB12936 6965872 6964130 580 1743 conserved hypothetical protein 5.3 - 1.32 0.18 14
7211 RB12959 6974205 6973882 107 324 conserved hypothetical protein 11.4 - 1.20 0.28 23<br />
7251 RB13038 7005061 7002800 753 2262 conserved hypothetical protein, membrane 6.9 - 1.32 0.09 7<br />
7256 RB13053 7008596 7009024 142 429 secreted protein 5.6 + 1.05 0.15 15<br />
7270 RB13082 7020677 7020898 73 222 conserved hypothetical protein 11.7 + 1.17 0.26 22<br />
7293 RB13115 7036614 7036369 81 246 conserved hypothetical protein 9.8 - 2.00 0.27 14<br />
7340 RB13202 7086189 7086052 45 138 conserved hypothetical protein 9.4 - 1.44 0.07 5<br />
7350 RB13221 7093018 7093986 322 969 secreted protein 4.5 + 1.56 0.13 8<br />
7351 RB13222 7094714 7094088 208 627 SOUL heme-binding protein 8.7 - 1.72 0.28 16<br />
7360 RB13238 7101410 7101904 164 495 TadE-like 5.3 + 3.52 0.12 3<br />
7361 RB13239 7102571 7101981 196 591 conserved hypothetical protein, membrane 9.5 - 1.74 0.18 10<br />
7370 RB13259 7108365 7106461 634 1905 conserved hypothetical protein 5.7 - 1.95 0.41 21<br />
300min ID Locus AA Nuc Product Gene EC IEP Strand Ratio Stdev %<br />
1004 RB1902 988719 990776 1.6.99.3 685 2058 NADH oxidase 1.6.99.3 9.3 + 1.20 0.14 9<br />
1007 RB1909 992934 993434 166 501 Globin 4.5 + 2.10 0.15 8<br />
1961 RB3596 1868895 1869329 144 435 nitrogen fixation protein (NifU protein) 4.3 + 1.08 0.20 8<br />
6831 RB12276 6605112 6605444 110 333 conserved hypothetical protein 4.7 + 2.88 0.18 20<br />
7243 RB13025 6997019 6996438 193 582 monooxygenase 9.7 - 1.20 0.05 9<br />
1212 RB2291 1199317 1200063 248 747 Sporulation initiation inhibitor protein soj soj 4.5 + 1.06 0.06 6<br />
1546 RB2860 1487828 1489987 719 2160 type IV pilus assembly protein PilM 4.5 + 1.20 0.08 10<br />
4149 RB7375 3948831 3947593 3.4.11.4 412 1239 Peptidase T pepT 3.4.11.4 4.4 - 1.18 0.23 18<br />
5034 RB8979 4785013 4786671 4.3.1.17 552 1659 L-serine dehydratase, iron-sulfur-dependent, alpha subunit 4.3.1.17 4.4 + 1.46 0.06 12<br />
5144 RB9200 4919220 4918369 3.1.3.41 283 852 4-nitrophenylphosphatase PHO13 3.1.3.41 4.9 - 1.58 0.23 9<br />
7094 RB12740 6865551 6864364 3.1.1.17 395 1188 gluconolactonase [precursor] gnl 3.1.1.17 4.8 - 1.12 0.15 5<br />
5878 RB10566 5734146 5732695 483 1452 Gluconate transporter 9.2 - 1.60 0.10 8<br />
4579 RB8123 4346827 4347360 4.2.1.- 177 534 (3R)-hydroxymyristoyl-[acyl carrier protein] dehydratase fabZ 4.2.1.- 4.9 + 1.05 0.13 19<br />
948 RB1790 926593 927168 191 576 RNA polymerase ECF-type sigma factor 10.4 + 1.48 0.13 8<br />
4744 RB8445 4514475 4514050 141 426 Penicillinase repressor 9.9 - 1.95 0.23 2<br />
5571 RB9999 5386828 5385983 281 846 conserved hypothetical protein 4.6 - 1.35 0.13 10<br />
5606 RB10049 5419507 5420160 217 654 RNA polymerase ECF-type sigma factor 10.1 + 1.27 0.12 1<br />
6160 RB11075 6005727 6005224 167 504 Bacterial regulatory protein, MarR 6.4 - 1.03 0.16 4<br />
6161 RB11077 6006371 6005904 155 468 Protein of unknown function, UPF0074 8.8 - 1.99 0.08 9<br />
4187 RB7437 3987953 3988672 3.4.21.88 239 720 LexA repressor lexA 3.4.21.88 9.8 + 1.73 0.15 20<br />
98 RB170 89333 89623 96 291 Transposase IS3/IS911 10.1 + 1.34 0.21 15<br />
99 RB171 89626 90504 292 879 ISxac3 transposase 10 + 1.29 0.14 11<br />
382 RB704 378137 378427 96 291 Transposase IS3/IS911 10.1 + 1.83 0.08 8<br />
383 RB706 378430 379308 292 879 ISxac3 transposase 10 + 1.64 0.13 10<br />
628 RB1189 606838 607128 96 291 Transposase IS3/IS911 10.1 + 1.81 0.07 15<br />
629 RB1190 607131 608009 292 879 integrase 10 + 1.74 0.26 7<br />
2213 RB3999 2064741 2063431 436 1311 site-specific recombinase, 10.4 - 1.21 0.08 1<br />
2381 RB4299 2214533 2214823 96 291 Transposase IS3/IS911 9.9 + 1.69 0.18 9<br />
2382 RB4300 2214826 2215704 292 879 integrase 10 + 1.56 0.14 9<br />
3319 RB5887 3087343 3086465 292 879 integrase 9.9 - 1.58 0.13 8<br />
3420 RB6070 3186811 3187416 201 606 HNH endonuclease family protein 9.9 + 1.63 0.13 16<br />
3474 RB6171 3239091 3238048 347 1044 site-specific recombinase 10.7 - 1.47 0.14 4<br />
3787 RB6709 3564146 3563856 96 291 Transposase IS3/IS911 10.1 - 1.71 0.08 9<br />
6558 RB11749 6328064 6328354 96 291 Transposase IS3/IS911 10.1 + 1.64 0.24 3<br />
6559 RB11750 6328357 6329235 292 879 integrase 10 + 2.02 0.06 2<br />
6577 RB11802 6361950 6362240 96 291 Transposase IS3/IS911 10.1 + 1.10 0.08 8<br />
6578 RB11803 6362243 6363121 292 879 ISxac3 transposase 10 + 1.27 0.10 19<br />
6789 RB12202 6579810 6578185 541 1626 integrase-recombinase protein 9.9 - 1.39 0.29 6<br />
7203 RB12940 6966246 6966536 96 291 Transposase IS3/IS911 10.1 + 1.81 0.17 11<br />
7204 RB12941 6966539 6967417 292 879 integrase 10 + 1.15 0.13 22<br />
1355 RB2507 1313064 1311886 4.2.1.46 392 1179 dTDP-glucose 4,6-dehydratase rfbB 4.2.1.46 5.2 - 1.01 0.13 8<br />
1727 RB3195 1650068 1648239 609 1830 carboxy-terminal processing protease 6.1 - 1.31 0.18 5<br />
5719 RB10255 5542656 5543669 337 1014 MscS Mechanosensitive ion channel 5.4 + 1.56 0.10 9<br />
7070 RB12690 6834088 6833336 2.7.7.38 250 753 3-deoxy-manno-octulosonate cytidylyltransferase kdsB 2.7.7.38 4.7 - 1.54 0.13 14<br />
6711 RB12053 6502558 6502199 119 360 biopolymer transport protein, ExbD/TolR family 4.2 - 1.08 0.18 6<br />
6712 RB12055 6503587 6502628 319 960 MotA/TolQ/ExbB proton channel family protein 5.3 - 1.11 0.07 23<br />
7115 RB12773 6884826 6885932 368 1107 twitching motility protein PilT 6.3 + 1.17 0.20 6<br />
7118 RB12781 6888383 6889822 479 1440 pilus assembly protein PilC 5.6 + 1.21 0.08 5<br />
731 RB1359 693488 695377 3.4.21.- 629 1890 Protease do precursor degP 3.4.21.- 6.1 + 1.16 0.12 11<br />
3529 RB6278 3302303 3297678 1541 4626 Putative Ig 3.6 - 2.20 0.08 4<br />
3812 RB6751 3586111 3588690 859 2580 chaperone ClpB clpB 5.2 + 1.21 0.10 13<br />
6727 RB12080 6517231 6515753 2.1.1.77 492 1479 Protein-L-isoaspartate(D-aspartate) O-methyltransferase 2.1.1.77 8.9 - 1.09 0.25 5<br />
212 RB370 184428 186089 553 1662 nitrate transporter substrate-binding protein 4.6 + 2.96 0.11 4<br />
214 RB373 187285 188283 332 999 ABC transporter, ATP-binding protein 9.9 + 1.50 0.09 6<br />
220 RB380 196136 197740 1.8.1.2 534 1605 sulfite reductase [NADPH] flavoprotein cysJ 1.8.1.2 4.4 + 1.54 0.04 3<br />
766 RB1433 732848 732411 145 438 Na(+):H(+) antiporter subunit B mrpB 9.2 - 1.03 0.18 5<br />
791 RB1477 755327 756943 3.1.6.8 538 1617 arylsulfatase precursor ARSA 3.1.6.8 5.5 + 1.62 0.09 14<br />
2817 RB5003 2572662 2574158 498 1497 Rh-like protein/ammonium transporter AMT1.4 4 + 1.92 0.18 0<br />
2984 RB5294 2732584 2730983 533 1602 sulfatase 5.6 - 1.20 0.16 9<br />
5967 RB10727 5830775 5831605 276 831 manganese-containing catalase 5 + 1.41 0.24 13<br />
6128 RB11009 5969752 5969036 238 717 sulfite reductase-like flavoprotein 3.9 - 1.13 0.06 11<br />
6675 RB11981 6459460 6460413 317 954 ABC transporter (substrate-binding protein) 4.8 + 1.41 0.26 3<br />
7165 RB12858 6929710 6928649 353 1062 oligopeptide transport ATP-binding protein OppF oppF 7 - 1.14 0.12 22<br />
7169 RB12862 6934893 6932926 655 1968 Oligopeptide-binding protein oppA oppA 4.5 - 1.10 0.25 8<br />
4332 RB7678 4123339 4122659 226 681 protocatechuate 3,4-dioxygenase type II beta subunit 9.4 - 1.25 0.11 10<br />
1347 RB2498 1305777 1304887 296 891 methyltransferase, 6.2 - 1.10 0.12 13<br />
557 RB1044 543422 544231 269 810 TatD-related deoxyribonuclease 5.2 + 1.23 0.19 12<br />
835 RB1555 797278 795848 476 1431 dehydrogenases and related proteins 5.8 - 1.02 0.03 4<br />
2896 RB5140 2641937 2643778 613 1842 hypothetical protein 5.5 + 1.29 0.21 14<br />
4603 RB8173 4366881 4367900 339 1020 MoxR protein 5.1 + 1.51 0.09 5<br />
4796 RB8533 4552172 4552762 3.2.-.- 196 591 protease I pfpI 3.2.-.- 4.8 + 1.63 0.24 6<br />
5109 RB9132 4886727 4888004 425 1278 conserved hypothetical protein 5.5 + 1.40 0.08 3<br />
5568 RB9992 5383545 5382085 486 1461 SufBD 5.9 - 1.07 0.25 10<br />
5721 RB10258 5544584 5545453 3.1.3.2 289 870 Survival protein SurE 3.1.3.2 5.3 + 1.22 0.11 3<br />
5815 RB10440 5655693 5655842 49 150 hypothetical protein 11.5 + 1.31 0.05 8<br />
6215 RB11173 6063194 6063586 130 393 conserved hypothetical protein, secreted 4.7 + 1.56 0.18 4<br />
6216 RB11174 6065046 6063637 469 1410 metallo-beta-lactamase family protein 4.8 - 1.08 0.05 10<br />
6641 RB11923 6419736 6419041 231 696 Electron transport protein SCO1/SenC 4.3 - 1.30 0.14 2<br />
6667 RB11964 6444840 6445160 106 321 conserved hypothetical protein 3.9 + 1.04 0.20 18<br />
7369 RB13257 7105590 7106450 3.7.1.- 286 861 2-hydroxy-6-ketonona-2,4-dienedioic acid hydrolase bphD 3.7.1.- 4.6 + 1.03 0.07 7<br />
324 RB560 309384 308542 280 843 protein containing DGPFAETKE domain DGPF 4.9 - 1.34 0.11 8<br />
2453 RB4419 2272912 2273448 178 537 MgtC/SapB transporter 6.2 + 1.09 0.10 7<br />
4648 RB8260 4415394 4415068 108 327 protein containing DUF167 10.5 - 1.31 0.06 17<br />
5413 RB9699 5226252 5228345 697 2094 Rhs element Vgr protein 4.2 + 1.66 0.03 19<br />
6217 RB11176 6065605 6065144 153 462 protein containing DUF442 4.8 - 1.63 0.16 15<br />
80 RB140 61578 64541 2.7.1.37 987 2964 serine/threonine protein kinase pkn3 2.7.1.37 5.2 + 1.09 0.24 22<br />
3259 RB5780 3028975 3029472 165 498 two-component response regulator 6.2 + 2.09 0.23 8<br />
4202 RB7461 3999634 4001478 614 1845 hypothetical protein 4.4 + 1.27 0.25 6<br />
5788 RB10388 5613357 5615438 2.7.1.37 693 2082 protein kinase KIN11 2.7.1.37 9 + 1.19 0.10 7<br />
6679 RB11986 6465612 6462064 1182 3549 two-component sensor/response regulator hybrid 6.4 - 1.04 0.03 18<br />
7209 RB12952 6972002 6972667 221 666 two-component system, regulatory protein 4.7 + 1.53 0.32 2<br />
7291 RB13112 7036128 7034035 697 2094 Hybrid sensor histidine kinase 5.5 - 1.31 0.11 7<br />
4 RB5 1514 399 371 1116 secreted protein 4.1 - 2.35 0.29 12<br />
50 RB85 31507 32739 410 1233 conserved hypothetical protein, secreted 5.1 + 1.10 0.10 9<br />
79 RB138 60872 61516 214 645 Sigma factor, ECF-like 4.8 + 1.93 0.14 7<br />
90 RB160 84284 84027 85 258 hypothetical protein 10.7 - 1.62 0.22 14<br />
101 RB174 91448 91585 45 138 hypothetical protein 5.2 + 1.64 0.17 10<br />
152 RB275 135819 135370 149 450 hypothetical protein 9.3 - 1.16 0.09 8<br />
300 RB520 284989 286116 375 1128 conserved hypothetical protein 4.8 + 1.02 0.12 12<br />
301 RB521 286399 286208 63 192 hypothetical protein 10.7 - 2.06 0.11 5<br />
392 RB723 388051 388233 60 183 hypothetical protein 7.3 + 2.37 0.24 9<br />
469 RB864 458119 458598 159 480 hypothetical protein 6.1 + 1.42 0.13 6<br />
470 RB865 458731 460359 542 1629 hypothetical protein 3.1 + 1.69 0.11 17<br />
471 RB867 460731 460429 100 303 hypothetical protein 9.7 - 1.47 0.25 4<br />
528 RB968 507840 507079 253 762 protein containing DUF1596 11.3 - 1.25 0.05 5<br />
530 RB971 509411 508101 436 1311 Patatin-like phospholipase 10.4 - 1.06 0.06 14<br />
556 RB1043 543083 543352 89 270 conserved hypothetical protein 4.7 + 1.41 0.20 15<br />
599 RB1138 585492 585959 155 468 conserved hypothetical protein 10.5 + 1.54 0.19 9<br />
619 RB1174 603031 603498 155 468 secreted protein 9.8 + 1.88 0.17 1
622 RB1179 603998 604384 128 387 hypothetical protein 12 + 2.03 0.01 7<br />
623 RB1180 604378 604656 92 279 hypothetical protein 12.6 + 1.69 0.12 4<br />
638 RB1201 612754 612629 41 126 hypothetical protein 12.5 - 1.31 0.10 12<br />
644 RB1210 617416 616571 281 846 AP endonuclease 2, C-terminal 4.5 - 2.51 0.31 3<br />
662 RB1239 632500 632033 155 468 hypothetical protein 9.7 - 1.43 0.05 15<br />
668 RB1254 640610 640747 45 138 hypothetical protein 10.6 + 2.44 0.36 11<br />
692 RB1294 657236 657051 61 186 hypothetical protein 9.8 - 1.75 0.19 5<br />
707 RB1322 671526 671633 35 108 hypothetical protein 12.8 + 1.49 0.07 5<br />
725 RB1352 686463 687803 446 1341 hypothetical protein 5.5 + 1.04 0.05 10<br />
747 RB1394 711994 711758 78 237 hypothetical protein 10.1 - 1.21 0.13 6<br />
748 RB1395 712097 713056 319 960 secreted protein 5.5 + 1.30 0.08 18<br />
792 RB1478 756943 758943 666 2001 hypothetical protein 5.3 + 1.88 0.26 6<br />
818 RB1522 777494 778657 387 1164 protein containing DUF1559 5.6 + 1.69 0.10 10<br />
819 RB1523 778654 779130 158 477 hypothetical protein 7.3 + 1.81 0.18 16<br />
823 RB1531 782534 783889 451 1356 membrane protein 4.5 + 1.29 0.21 3<br />
836 RB1557 797241 797420 59 180 hypothetical protein 11.8 + 1.60 0.07 5<br />
868 RB1617 834021 833623 132 399 hypothetical protein 6.8 - 1.13 0.05 9<br />
910 RB1716 895805 895557 82 249 protein containing DUF1589 10.1 - 1.28 0.12 23<br />
916 RB1729 901146 900784 120 363 hypothetical protein 7.7 - 1.19 0.28 12<br />
927 RB1750 909661 909837 58 177 hypothetical protein 10.1 + 1.25 0.15 13<br />
936 RB1767 919059 916336 907 2724 60-kDa cysteine-rich outer membrane protein precursor omcB 4.2 - 1.49 0.19 2<br />
937 RB1771 919524 919273 83 252 hypothetical protein 7.6 - 1.39 0.03 13<br />
947 RB1789 925861 926592 243 732 conserved hypothetical protein 9.4 + 1.10 0.14 9<br />
985 RB1872 966053 966169 38 117 hypothetical protein 12.3 + 1.96 0.15 12<br />
1006 RB1908 992277 992900 207 624 secreted protein 4.6 + 1.42 0.13 7<br />
1008 RB1910 993764 993393 123 372 hypothetical protein 11.2 - 1.93 0.15 16<br />
1142 RB2173 1147959 1146895 354 1065 hypothetical protein 6.3 - 2.25 0.37 10<br />
1185 RB2252 1181701 1181540 53 162 hypothetical protein 9.8 - 1.67 0.17 6<br />
1207 RB2283 1197719 1197102 205 618 protein containing DUF1590 10.5 - 2.03 0.12 6<br />
1223 RB2310 1209545 1209222 107 324 hypothetical protein 11.5 - 1.23 0.07 17<br />
1313 RB2457 1279788 1280144 118 357 hypothetical protein 12.7 + 1.15 0.20 18<br />
1346 RB2497 1304778 1304906 42 129 hypothetical protein 6.7 + 1.19 0.22 11<br />
1365 RB2517 1316854 1317012 52 159 hypothetical protein 10.3 + 1.87 0.15 3<br />
1370 RB2524 1319449 1319796 115 348 hypothetical protein 7.4 + 1.28 0.04 16<br />
1380 RB2545 1328931 1328773 52 159 hypothetical protein 11.2 - 1.86 0.30 9<br />
1435 RB2649 1374987 1375148 53 162 hypothetical protein 9.1 + 1.39 0.12 16<br />
1461 RB2697 1402745 1404271 508 1527 protein containing DUF1501 6.9 + 1.32 0.22 22<br />
1506 RB2784 1448065 1447454 203 612 membrane protein containing DUF107 4.5 - 1.23 0.28 14<br />
1515 RB2804 1458645 1458040 201 606 hypothetical protein 5.3 - 1.38 0.19 12<br />
1518 RB2809 1459745 1459065 226 681 hypothetical protein 9.1 - 1.06 0.13 8<br />
1524 RB2822 1463434 1464933 499 1500 secreted protein containing DUF1598 6.9 + 1.52 0.13 8<br />
1528 RB2829 1467448 1467564 38 117 hypothetical protein 4.1 + 1.17 0.09 7<br />
1549 RB2867 1491909 1494002 697 2094 hypothetical protein 4.7 + 1.91 0.18 6<br />
1645 RB3034 1572089 1569573 838 2517 secreted protein containing DUF1585 5.7 - 1.11 0.06 9<br />
1646 RB3036 1573582 1572092 496 1491 protein containing DUF1552 9.1 - 1.52 0.14 5<br />
1656 RB3052 1577799 1577692 35 108 hypothetical protein 11.2 - 1.57 0.08 9<br />
1695 RB3140 1620838 1621173 111 336 hypothetical protein 10.2 + 1.06 0.09 10<br />
1705 RB3152 1626667 1626461 68 207 hypothetical protein 12.2 - 1.21 0.12 4<br />
1706 RB3153 1626911 1626681 76 231 hypothetical protein (protein containing DUF1584) 12.4 - 2.20 0.09 5<br />
1707 RB3154 1627155 1626925 76 231 hypothetical protein 12.7 - 1.93 0.10 6<br />
1708 RB3155 1627430 1627149 93 282 hypothetical protein 12.7 - 2.69 0.16 10<br />
1711 RB3159 1629337 1629116 73 222 protein containing DUF1590 12.1 - 1.66 0.17 4<br />
1722 RB3189 1646085 1646393 102 309 hypothetical protein 11.1 + 1.49 0.06 13<br />
1728 RB3196 1650093 1650422 109 330 hypothetical protein 10.1 + 1.98 0.10 19<br />
1788 RB3299 1693917 1692925 330 993 protein containing DUF1559 6.3 - 1.68 0.31 7<br />
1809 RB3333 1713409 1713528 39 120 hypothetical protein 12.2 + 1.04 0.07 15<br />
1827 RB3365 1730124 1730255 43 132 hypothetical protein 11.4 + 1.03 0.16 6<br />
1853 RB3406 1759455 1759589 44 135 hypothetical protein 10.5 + 1.01 0.06 6<br />
1865 RB3426 1771404 1771622 72 219 hypothetical protein 12.4 + 1.86 0.11 17<br />
1868 RB3430 1772646 1773392 248 747 conserved hypothetical protein, membrane 7.5 + 1.03 0.18 18<br />
2005 RB3659 1900428 1900607 59 180 hypothetical protein 10.7 + 3.01 0.25 7<br />
2272 RB4114 2117690 2117971 93 282 protein containing DUF1582 11.1 + 1.76 0.01 6<br />
2308 RB4185 2151951 2152220 89 270 hypothetical protein 12 + 1.75 0.11 11<br />
2388 RB4306 2221207 2219834 457 1374 transposase and inactivated derivative 8.4 - 1.20 0.11 5<br />
2395 RB4320 2226114 2226611 165 498 hypothetical protein 12 + 1.06 0.05 2<br />
2440 RB4397 2263976 2264143 55 168 protein containing DUF1560 10.5 + 1.92 0.04 9<br />
2584 RB4642 2377301 2377558 85 258 conserved hypothetical protein 4.4 + 1.37 0.10 13<br />
2597 RB4658 2382780 2382199 193 582 inosine monophosphate dehydrogenase-related protein 4.8 - 2.03 0.26 11<br />
2615 RB4694 2396117 2397352 411 1236 protein containing DUF1559 6.5 + 1.81 0.20 11<br />
2616 RB4697 2397357 2397539 60 183 secreted protein 3.1 + 1.96 0.22 18<br />
2629 RB4715 2413294 2417169 1291 3876 conserved hypothetical protein 5.3 + 1.09 0.19 6<br />
2654 RB4750 2435462 2436076 204 615 membrane protein 10 + 1.06 0.07 23<br />
2694 RB4810 2465774 2465418 118 357 hypothetical protein 10.8 - 1.05 0.24 7<br />
2783 RB4957 2548380 2548553 57 174 hypothetical protein 12.4 + 1.07 0.07 6<br />
2792 RB4969 2554443 2555447 334 1005 hypothetical protein 10 + 1.65 0.10 2<br />
2816 RB5001 2572354 2572665 103 312 hypothetical protein 10.3 + 2.62 0.04 9<br />
2819 RB5007 2574916 2575812 3.1.1.- 298 897 esterase 6 + 1.59 0.00 10<br />
2894 RB5138 2641024 2640848 58 177 hypothetical protein 12.4 - 2.31 0.22 16<br />
3009 RB5347 2764290 2764442 50 153 hypothetical protein 11.9 + 1.32 0.12 13<br />
3010 RB5348 2765787 2764414 457 1374 transposase and inactivated derivative 8.4 - 1.45 0.18 6<br />
3022 RB5370 2774826 2773897 309 930 Rhodopirellula transposase 5.9 - 2.11 0.12 21<br />
3117 RB5522 2860696 2860331 121 366 hypothetical protein 10.3 - 1.35 0.28 11<br />
3130 RB5551 2889101 2891092 663 1992 hypothetical protein 5.7 + 2.16 0.23 19<br />
3139 RB5567 2897766 2897566 66 201 hypothetical protein 12.1 - 1.55 0.29 4<br />
3142 RB5572 2901618 2901499 39 120 hypothetical protein 13 - 2.17 0.09 8<br />
3233 RB5737 2994415 2994777 120 363 hypothetical protein 11.9 + 1.12 0.09 11<br />
3320 RB5888 3087636 3087346 96 291 Transposase IS3/IS911 10.1 - 2.02 0.16 16<br />
3345 RB5937 3112147 3113067 306 921 hypothetical protein 11.7 + 1.10 0.18 15<br />
3346 RB5938 3113484 3112372 370 1113 hypothetical protein 5.6 - 1.28 0.19 8<br />
3426 RB6081 3191035 3190877 52 159 hypothetical protein 11.5 - 1.49 0.23 6<br />
3449 RB6131 3223482 3223790 102 309 hypothetical protein 12.1 + 1.57 0.10 11<br />
3457 RB6144 3228305 3228189 38 117 hypothetical protein 11.1 - 1.78 0.20 10<br />
3482 RB6188 3244384 3244178 68 207 hypothetical protein 9.6 - 2.22 0.09 7<br />
3498 RB6221 3264012 3260995 1005 3018 conserved hypothetical protein, secreted 4.8 - 1.07 0.08 4<br />
3585 RB6375 3349681 3350574 297 894 hypothetical protein 8.7 + 1.68 0.07 19<br />
3591 RB6382 3355162 3355043 39 120 hypothetical protein 10.4 - 1.03 0.19 2<br />
3640 RB6457 3407990 3407787 67 204 hypothetical protein 11.7 - 1.00 0.02 18<br />
3668 RB6497 3442109 3442804 231 696 conserved hypothetical protein 3.9 + 1.01 0.18 18<br />
3756 RB6661 3538752 3538486 88 267 hypothetical protein 10.6 - 1.07 0.19 5<br />
3893 RB6903 3669220 3669059 53 162 hypothetical protein 9.3 - 1.42 0.18 4<br />
3894 RB6904 3669200 3670501 433 1302 secreted protein containing DUF1552 7.1 + 1.68 0.07 3<br />
3900 RB6910 3671484 3671356 42 129 hypothetical protein 9.5 - 3.33 0.09 7<br />
3902 RB6912 3672793 3672960 55 168 secreted protein 9 + 2.38 0.16 3<br />
3907 RB6920 3676847 3677914 355 1068 protein containing DUF1559 6.5 + 1.92 0.05 17<br />
3909 RB6925 3678461 3677976 161 486 hypothetical protein 9.8 - 2.17 0.38 8<br />
3910 RB6928 3678479 3678961 160 483 hypothetical protein 4.1 + 2.64 0.20 15<br />
3941 RB6990 3719456 3719166 96 291 hypothetical protein 11.9 - 1.62 0.24 3<br />
3969 RB7039 3743573 3742965 202 609 hypothetical protein 7.9 - 1.18 0.04 19<br />
4151 RB7381 3950326 3949595 243 732 membrane protein 7.4 - 1.00 0.18 13<br />
4158 RB7390 3954342 3954968 208 627 hypothetical protein 12.2 + 1.77 0.23 5<br />
4162 RB7395 3955956 3956351 131 396 hypothetical protein 11.9 + 1.12 0.05 9<br />
4203 RB7464 4001614 4001480 44 135 hypothetical protein 10.2 - 1.14 0.07 5<br />
4288 RB7603 4091070 4088560 836 2511 outer membrane efflux protein 5.1 - 1.41 0.07 12<br />
4291 RB7612 4092907 4093317 136 411 hypothetical protein 11.4 + 1.16 0.14 14<br />
4318 RB7651 4112517 4112383 44 135 hypothetical protein 6.7 - 1.43 0.20 9<br />
4398 RB7786 4172318 4171854 154 465 hypothetical protein 10.5 - 1.47 0.14 8<br />
4465 RB7901 4221613 4221293 106 321 hypothetical protein 11.8 - 1.79 0.14 17<br />
4497 RB7960 4257723 4256632 2.7.3.- 363 1092 ATP:guanido phosphotransferase 2.7.3.- 5.4 - 1.12 0.19 13<br />
4586 RB8138 4351960 4352187 75 228 hypothetical protein 4.1 + 1.26 0.24 2<br />
4590 RB8146 4355122 4354667 151 456 hypothetical protein 4.3 - 1.45 0.03 6<br />
4705 RB8367 4463905 4464057 50 153 hypothetical protein 3.7 + 1.14 0.19 6<br />
4715 RB8385 4479935 4480087 50 153 hypothetical protein 10.1 + 2.24 0.14 11<br />
4727 RB8409 4495547 4495840 97 294 hypothetical protein 8.9 + 1.06 0.12 12
4791 RB8527 4549097 4550089 330 993 protein containing DUF1559 6.6 + 1.90 0.04 7<br />
4793 RB8529 4550288 4550716 142 429 secreted protein 4.5 + 1.59 0.11 17<br />
4794 RB8530 4550886 4550725 53 162 hypothetical protein 12.3 - 1.43 0.24 15<br />
4852 RB8641 4605970 4606248 92 279 hypothetical protein 7.5 + 2.42 0.15 4<br />
5062 RB9038 4823103 4822642 153 462 hypothetical protein 10.5 - 1.05 0.12 6<br />
5072 RB9067 4839661 4839834 57 174 secreted protein 3.4 + 1.79 0.11 8<br />
5074 RB9070 4840267 4841496 409 1230 secreted protein containing DUF1559 8.6 + 3.75 0.30 6<br />
5120 RB9155 4896042 4895920 40 123 hypothetical protein 10.4 - 1.38 0.04 3<br />
5131 RB9174 4908450 4907485 321 966 protein containing DUF1559 6.7 - 1.62 0.05 7<br />
5143 RB9199 4918323 4918120 67 204 hypothetical protein 11.9 - 1.15 0.09 14<br />
5157 RB9230 4936673 4936996 107 324 hypothetical protein 9.9 + 1.73 0.16 6<br />
5217 RB9346 5008335 5008550 71 216 hypothetical protein 10.5 + 1.93 0.12 5<br />
5218 RB9347 5008600 5008394 68 207 hypothetical protein 9.9 - 1.87 0.10 5<br />
5230 RB9365 5017351 5017809 152 459 hypothetical protein 11.6 + 1.71 0.08 2<br />
5455 RB9786 5270645 5270205 146 441 hypothetical protein 12 - 1.22 0.24 12<br />
5473 RB9811 5284365 5284673 102 309 secreted protein 11.7 + 1.61 0.20 13<br />
5550 RB9955 5363605 5362247 452 1359 secreted protein containing DUF1552 5.6 - 1.48 0.19 23<br />
5647 RB10126 5465210 5465341 43 132 hypothetical protein 12.9 + 1.38 0.02 0<br />
5654 RB10137 5469772 5469551 73 222 protein containing DUF1590 12.1 - 2.12 0.00 7<br />
5717 RB10249 5541332 5539851 493 1482 conserved hypothetical protein, secreted 5.5 - 1.50 0.10 6<br />
5738 RB10292 5559646 5561391 581 1746 conserved hypothetical protein, secreted 8.3 + 2.01 0.06 1<br />
5739 RB10295 5562427 5561501 308 927 methylene tetrahydrofolate mtdC 5.2 - 1.53 0.02 8<br />
5789 RB10391 5615419 5616087 222 669 membrane protein 4.3 + 1.06 0.08 4<br />
5826 RB10462 5674183 5674386 67 204 hypothetical protein 10.6 + 1.47 0.11 12<br />
5834 RB10473 5679433 5681868 811 2436 conserved hypothetical protein 5.4 + 1.70 0.20 6<br />
5902 RB10608 5759903 5761276 457 1374 transposase and inactivated derivative 8.4 + 1.58 0.12 5<br />
5931 RB10665 5789912 5790514 200 603 hypothetical protein 5.3 + 1.40 0.07 12<br />
5942 RB10682 5807422 5805998 474 1425 secreted protein 12.2 - 1.45 0.18 17<br />
6043 RB10861 5896851 5896991 46 141 hypothetical protein 4.2 + 1.46 0.19 7<br />
6124 RB11000 5964054 5963380 224 675 conserved hypothetical protein, membrane 8.8 - 1.41 0.10 5<br />
6129 RB11010 5970585 5969749 278 837 conserved hypothetical protein, secreted 4.9 - 1.05 0.11 15<br />
6165 RB11082 6007371 6007033 112 339 hypothetical protein 10.9 - 1.75 0.16 7<br />
6176 RB11107 6021425 6022102 225 678 secreted protein 6.5 + 1.43 0.10 11<br />
6178 RB11110 6022600 6023529 309 930 Rhodopirellula transposase 5.8 + 2.07 0.23 12<br />
6236 RB11209 6080603 6080914 103 312 hypothetical protein 9.5 + 1.71 0.26 13<br />
6382 RB11446 6199492 6199833 113 342 conserved hypothetical protein 10 + 1.41 0.18 3<br />
6406 RB11475 6209468 6209641 57 174 conserved hypothetical protein 4.7 + 2.30 0.08 13<br />
6411 RB11484 6212742 6212611 43 132 conserved hypothetical protein 5.5 - 1.12 0.15 7<br />
6424 RB11504 6221285 6221067 72 219 conserved hypothetical protein 10.7 - 1.17 0.08 25<br />
6455 RB11566 6243111 6243698 195 588 conserved hypothetical protein 10.8 + 1.23 0.30 18<br />
6470 RB11599 6256485 6255736 249 750 conserved hypothetical protein 4.4 - 1.02 0.18 5<br />
6472 RB11603 6256722 6256835 37 114 conserved hypothetical protein 9 + 1.11 0.06 13<br />
6474 RB11607 6258999 6260507 502 1509 cytoskeleton assembly control protein 3.3 + 1.03 0.13 12<br />
6475 RB11612 6260715 6260470 81 246 conserved hypothetical protein 12 - 2.00 0.23 14<br />
6534 RB11698 6308550 6307780 256 771 conserved hypothetical protein, secreted 4.1 - 1.88 0.26 15<br />
6571 RB11768 6334808 6334939 43 132 conserved hypothetical protein 9.4 + 1.44 0.03 8<br />
6585 RB11813 6366331 6366573 80 243 conserved hypothetical protein 13 + 1.70 0.33 9<br />
6637 RB11918 6415701 6415297 134 405 protein containing DUF971 6.1 - 1.73 0.15 11<br />
6660 RB11951 6437923 6438510 195 588 conserved hypothetical protein 6.1 + 1.49 0.03 19<br />
6688 RB12005 6475396 6475013 127 384 conserved hypothetical protein 3.6 - 1.21 0.21 17<br />
6760 RB12150 6556901 6557062 53 162 conserved hypothetical protein 4.1 + 1.61 0.07 13<br />
6786 RB12200 6577955 6577800 51 156 conserved hypothetical protein 11 - 1.30 0.16 21<br />
6898 RB12388 6667206 6665905 433 1302 ISXo8 transposase 9.4 - 2.25 0.44 10<br />
6899 RB12390 6668063 6667344 239 720 secreted protein containing DUF1555 4.1 - 1.95 0.19 9<br />
6915 RB12416 6679578 6680456 292 879 secreted protein 6 + 1.24 0.11 8<br />
6947 RB12461 6708942 6707206 578 1737 conserved hypothetical protein, secreted 4.2 - 1.80 0.15 3<br />
6950 RB12471 6712442 6712185 85 258 conserved hypothetical protein 9.7 - 2.34 0.06 22<br />
6951 RB12473 6712524 6712706 60 183 conserved hypothetical protein 7.3 + 1.51 0.34 19<br />
6952 RB12475 6712706 6715966 1086 3261 conserved hypothetical protein 5.5 + 1.20 0.23 10<br />
6954 RB12479 6717557 6716985 190 573 conserved hypothetical protein 5.8 - 1.27 0.13 7<br />
7033 RB12629 6799726 6799598 42 129 conserved hypothetical protein 10.7 - 1.37 0.10 15<br />
7053 RB12662 6817193 6817408 71 216 conserved hypothetical protein 11 + 1.03 0.16 9<br />
7066 RB12684 6831038 6830874 54 165 conserved hypothetical protein 5.4 - 2.68 0.23 9<br />
7076 RB12697 6837436 6842547 1703 5112 conserved hypothetical protein 3.6 + 1.59 0.22 16<br />
7088 RB12727 6860301 6858259 680 2043 conserved hypothetical protein 5.7 - 1.02 0.16 7<br />
7090 RB12732 6862068 6860800 422 1269 secreted protein containing DUF1501 6.3 - 1.82 0.14 14<br />
7091 RB12734 6863889 6862186 567 1704 conserved hypothetical protein, secreted 5.2 - 1.19 0.16 1<br />
7092 RB12737 6863917 6864114 65 198 conserved hypothetical protein 9.1 + 1.98 0.02 14<br />
7113 RB12770 6884806 6884306 166 501 conserved hypothetical protein 11.9 - 2.33 0.12 17<br />
7119 RB12782 6889986 6889819 55 168 conserved hypothetical protein 10.9 - 2.38 0.13 8<br />
7120 RB12783 6889970 6891277 435 1308 conserved hypothetical protein 4.5 + 1.58 0.12 3<br />
7126 RB12797 6905009 6905182 57 174 conserved hypothetical protein 7.3 + 1.37 0.04 9<br />
7130 RB12803 6909112 6908939 57 174 conserved hypothetical protein 10.2 - 1.59 0.14 22<br />
7133 RB12808 6912350 6911241 369 1110 secreted protein 4.6 - 1.58 0.36 11<br />
7134 RB12810 6912339 6912542 67 204 conserved hypothetical protein 11.3 + 1.03 0.11 23<br />
7147 RB12827 6919729 6919361 122 369 conserved hypothetical protein, membrane 12.5 - 1.24 0.29 10<br />
7148 RB12829 6919813 6920292 159 480 conserved hypothetical protein 9.5 + 1.04 0.10 10<br />
7170 RB12863 6934850 6935002 50 153 conserved hypothetical protein 12.6 + 1.21 0.10 14<br />
7197 RB12925 6960998 6960879 39 120 protein containing DUF1560 9.9 - 1.17 0.16 9<br />
7207 RB12946 6969668 6970402 244 735 conserved hypothetical protein 4.5 + 1.04 0.23 21<br />
7211 RB12959 6974205 6973882 107 324 conserved hypothetical protein 11.4 - 1.73 0.04 4<br />
7381 RB13283 7122232 7122041 63 192 conserved hypothetical protein 9.6 - 1.28 0.09 12<br />
7385 RB13294 7127129 7127677 182 549 conserved hypothetical protein 10.2 + 1.25 0.15 1<br />
7388 RB13300 7130249 7130061 62 189 conserved hypothetical protein 10.5 - 1.42 0.02 12<br />
7403 RB13327 7143601 7142336 421 1266 protein containing DUF1559 6.9 - 2.88 0.33
Salinity repressed<br />
10min ID Locus AA Nuc START STOP Product Gene EC IEP Strand Ratio Stdev %<br />
4093 RB7247 366 1101 3850538 3851638 glutamine synthetase II glnII 6.3.1.2 4.8 + -1.27 0.18 14<br />
4609 RB8188 259 780 4378573 4379352 hydroxypyruvate isomerase hyi 5.3.1.22 4.4 + -1.14 0.01 1<br />
3959 RB7022 90 273 3735291 3735563 Ribosomal protein S20 12.3 + -1.13 0.26 23<br />
4016 RB7117 181 546 3780322 3779777 Ribosomal protein L35 11.4 - -1.21 0.05 4<br />
4425 RB7833 228 687 4192692 4193378 Ribosomal protein L3 11 + -1.28 0.32 25<br />
4426 RB7834 222 669 4193390 4194058 Ribosomal protein L4/L1e 10.7 + -1.86 0.49 26<br />
4427 RB7836 107 324 4194060 4194383 Ribosomal L23 protein 10.8 + -2.36 0.76 32<br />
4428 RB7837 286 861 4194481 4195341 Ribosomal protein L2 11.8 + -1.87 0.05 3<br />
4430 RB7839 119 360 4195706 4196065 Ribosomal protein L22/L17 11 + -1.88 0.07 4<br />
4438 RB7854 61 186 4199624 4199809 Ribosomal protein S14 11.8 + -2.40 0.97 41<br />
4463 RB7899 141 426 4220226 4220651 50S ribosomal protein L11 rplK 9.6 + -1.24 0.05 4<br />
4750 RB8459 55 168 4518496 4518663 30S ribosomal protein S18 rpsR 10.5 + -1.50 0.33 22<br />
4892 RB8725 54 165 4638301 4638465 50S ribosomal protein L33 rpmG 10.6 + -1.15 0.09 8<br />
5832 RB10471 151 456 5678759 5678304 Ribosomal protein L13 10 - -1.63 0.64 39<br />
7145 RB12824 146 441 6917743 6917303 Ribosomal protein S16 5.3 - -1.70 0.01 0<br />
7154 RB12839 225 678 6921934 6922611 Ribosomal protein L1 9.8 + -1.98 0.58 29<br />
4459 RB7894 398 1197 4217484 4218680 translation elongation factor EF-Tu 5.2 + -2.39 0.61 26<br />
1030 RB1964 500 1503 1034475 1035977 hypothetical protein 4.7 + -1.19 0.11 9<br />
5531 RB9917 169 510 5344370 5343861 Single-strand binding protein ssb 5.3 - -1.28 0.20 16<br />
415 RB778 413 1242 418773 417532 secreted protein containing DUF1559 7 - -1.18 0.22 19<br />
583 RB1103 441 1326 572105 570780 capsule biosynthesis protein capB 6.3 - -1.08 0.02 2<br />
1130 RB2150 93 282 1138311 1138030 hypothetical protein 4.4 - -1.73 0.02 1<br />
3239 RB5745 130 393 3001234 3000842 hypothetical protein 10.7 - -1.78 0.05 3<br />
3271 RB5802 156 471 3039864 3040334 hypothetical protein 10.1 + -1.02 0.21 20<br />
5810 RB10431 323 972 5650397 5649426 conserved hypothetical protein 10.1 - -1.22 0.15 12<br />
7108 RB12760 50 153 6876908 6877060 conserved hypothetical protein 7.3 + -1.22 0.20 16<br />
7109 RB12761 54 165 6877026 6877190 conserved hypothetical protein 12 + -1.03 0.14 13<br />
20min ID Locus AA Nuc Product Gene EC IEP Strand Ratio Stdev %<br />
5700 RB10219 128 387 5521003 5521389 ATP synthase epsilon chain atpE 3.6.3.14 4.6 + -1.02 0.06 6<br />
179 RB314 309 930 156968 157897 malonyl CoA-acyl carrier protein transacylase fabD 2.3.1.39 4.4 + -1.45 0.36 25<br />
2733 RB4876 397 1194 2506214 2505021 delta 9 acyl-lipid fatty acid desaturase 1.14.19.- 9.5 - -1.25 0.22 18<br />
658 RB1233 206 621 629490 628870 30S ribosomal protein S4 rpsD 11.2 - -1.24 0.27 22<br />
4013 RB7114 347 1044 3779252 3778209 phenylalanyl-tRNA synthetase alpha chain pheS 6.1.1.20 4.6 - -1.05 0.27 25<br />
4015 RB7116 59 180 3779586 3779765 hypothetical protein 11.7 + -1.41 0.26 19<br />
4092 RB7246 50 153 3850393 3850545 hypothetical protein 9.7 + -1.05 0.15 14<br />
4314 RB7646 62 189 4111735 4111923 hypothetical protein 10.5 + -1.24 0.25 20<br />
4315 RB7647 73 222 4111931 4112152 hypothetical protein 7.4 + -1.91 0.10 5<br />
4413 RB7818 121 366 4185730 4186095 Ribosomal protein S12/S23 11.8 + -1.50 0.11 8<br />
4414 RB7820 157 474 4186170 4186643 Ribosomal protein S7 10.3 + -1.69 0.21 12<br />
4423 RB7829 108 327 4192031 4192357 Ribosomal protein S10 10.3 + -1.70 0.28 17<br />
4424 RB7832 50 153 4192499 4192651 hypothetical protein 13 + -1.67 0.25 15<br />
4425 RB7833 228 687 4192692 4193378 Ribosomal protein L3 11 + -2.01 0.11 6<br />
4427 RB7836 107 324 4194060 4194383 Ribosomal L23 protein 10.8 + -2.13 0.37 17<br />
4438 RB7854 61 186 4199624 4199809 Ribosomal protein S14 11.8 + -2.12 0.27 13<br />
4442 RB7859 177 534 4201351 4201884 Ribosomal protein S5 10.6 + -1.36 0.34 25<br />
4463 RB7899 141 426 4220226 4220651 50S ribosomal protein L11 rplK 9.6 + -1.10 0.10 9<br />
4750 RB8459 55 168 4518496 4518663 30S ribosomal protein S18 rpsR 10.5 + -2.05 0.31 15<br />
4824 RB8580 113 342 4577393 4577734 sigma-54 modulation protein 5.7 + -1.14 0.20 18<br />
5193 RB9304 81 246 4974378 4974623 Ribosomal protein L31 10.1 + -1.04 0.19 18<br />
5832 RB10471 151 456 5678759 5678304 Ribosomal protein L13 10 - -2.13 0.31 15<br />
7029 RB12623 127 384 6796904 6797287 Ribosomal protein S13 10.8 + -1.76 0.24 13<br />
7032 RB12628 210 633 6798878 6799510 50S ribosomal protein L17 rplQ 10.2 + -1.28 0.32 25<br />
7154 RB12839 225 678 6921934 6922611 Ribosomal protein L1 9.8 + -2.24 0.42 19<br />
7166 RB12859 361 1086 6930771 6929686 Oligopeptide transport ATP-binding protein oppD oppD 5.5 - -1.00 0.12 12<br />
5823 RB10458 206 621 5671130 5670510 Rnpc2-prov protein 10.3 - -1.52 0.07 4<br />
3528 RB6276 105 318 3297504 3297187 Histone-like bacterial DNA-binding protein 10.4 - -2.03 0.16 8<br />
3620 RB6428 164 495 3382816 3382322 conserved hypothetical protein, secreted 6.5 - -1.40 0.18 13<br />
6086 RB10934 68 207 5934135 5934341 CsbD-like 4.3 + -1.26 0.07 6<br />
581 RB1097 404 1215 570281 569067 membrane protein 5.3 - -1.31 0.21 16<br />
883 RB1644 123 372 845824 845453 secreted protein 9.7 - -1.05 0.15 14<br />
1505 RB2782 589 1770 1445576 1447345 protein containing DUF1559 7.2 + -1.24 0.14 11<br />
2312 RB4194 53 162 2156685 2156524 hypothetical protein 11.4 - -1.15 0.08 7<br />
3045 RB5409 97 294 2794195 2794488 hypothetical protein 12.7 + -1.41 0.25 18<br />
3588 RB6379 153 462 3353122 3352661 hypothetical protein 5.8 - -1.08 0.16 15<br />
3621 RB6429 61 186 3382998 3382813 hypothetical protein 12.1 - -1.29 0.29 22<br />
4589 RB8144 143 432 4354622 4354191 hypothetical protein 10.1 - -1.74 0.20 11<br />
4749 RB8457 113 342 4518458 4518117 hypothetical protein 11.5 - -1.11 0.07 6<br />
5280 RB9460 79 240 5078860 5078621 hypothetical protein 10.3 - -1.26 0.12 10<br />
5810 RB10431 323 972 5650397 5649426 conserved hypothetical protein 10.1 - -1.14 0.20 17<br />
6085 RB10932 65 198 5933872 5934069 secreted protein 3.8 + -1.20 0.08 7<br />
6094 RB10948 153 462 5938575 5939036 conserved hypothetical protein, secreted 4.2 + -1.74 0.11 7<br />
7153 RB12837 65 198 6921732 6921929 conserved hypothetical protein 9.8 + -1.75 0.35 20<br />
40min ID Locus AA Nuc Product Gene EC IEP Strand Ratio Stdev %<br />
2733 RB4876 397 1194 2506214 2505021 delta 9 acyl-lipid fatty acid desaturase 1.14.19.- 9.5 - -1.57 0.25 16<br />
3528 RB6276 105 318 3297504 3297187 Histone-like bacterial DNA-binding protein 10.4 - -2.82 0.09 3<br />
3620 RB6428 164 495 3382816 3382322 conserved hypothetical protein, secreted 6.5 - -1.78 0.21 12<br />
2312 RB4194 53 162 2156685 2156524 hypothetical protein 11.4 - -1.21 0.13 11<br />
3045 RB5409 97 294 2794195 2794488 hypothetical protein 12.7 + -1.83 0.48 26<br />
3621 RB6429 61 186 3382998 3382813 hypothetical protein 12.1 - -1.30 0.06 5<br />
4315 RB7647 73 222 4111931 4112152 hypothetical protein 7.4 + -1.71 0.39 23<br />
4413 RB7818 121 366 4185730 4186095 Ribosomal protein S12/S23 11.8 + -2.00 0.13 7<br />
4422 RB7828 40 123 4192037 4191915 hypothetical protein 10.4 - -1.15 0.17 15<br />
4611 RB8191 72 219 4382096 4382314 hypothetical protein 12 + -1.18 0.02 2<br />
4749 RB8457 113 342 4518458 4518117 hypothetical protein 11.5 - -1.47 0.09 6<br />
4861 RB8669 37 114 4614557 4614670 hypothetical protein 11.5 + -1.92 0.37 19<br />
5280 RB9460 79 240 5078860 5078621 hypothetical protein 10.3 - -1.99 0.30 15<br />
6094 RB10948 153 462 5938575 5939036 conserved hypothetical protein, secreted 4.2 + -1.15 0.22 19<br />
60min ID Locus AA Nuc Product Gene EC IEP Strand Ratio Stdev %<br />
5699 RB10217 479 1440 5519560 5520999 ATP synthase beta subunit 4.9 + -1.13 0.25 22<br />
1967 RB3603 344 1035 1875461 1874427 secreted protein 4.6 - -1.12 0.25 22<br />
2359 RB4269 282 849 2185049 2184201 glutamic acid specific endopeptidase, GSE=serine endopeptidase 5.6 - -1.13 0.31 28<br />
2432 RB4386 349 1050 2259814 2258765 CYSTEINE SYNTHASE A cysK 2.5.1.47 5.6 - -1.54 0.08 5<br />
4093 RB7247 366 1101 3850538 3851638 glutamine synthetase II glnII 6.3.1.2 4.8 + -1.90 0.24 13<br />
4459 RB7894 398 1197 4217484 4218680 translation elongation factor EF-Tu 5.2 + -3.10 0.15 5<br />
4756 RB8469 398 1197 4520779 4521975 lysine/ornithine decarboxylase ldc 4.1.1.18 5.2 + -1.22 0.07 6<br />
6865 RB12327 686 2061 6633918 6631858 TGF-beta receptor, type I/II extracellular region 4.5 - -1.64 0.21 13<br />
1423 RB2627 342 1029 1364551 1365579 Glyceraldehyde 3-phosphate dehydrogenase gap 1.2.1.12 5.4 + -1.50 0.05 4<br />
3774 RB6690 343 1032 3555887 3556918 fructose-bisphosphate aldolase fba 4.1.2.13 4.7 + -1.40 0.25 18<br />
4609 RB8188 259 780 4378573 4379352 hydroxypyruvate isomerase hyi 5.3.1.22 4.4 + -1.21 0.13 11<br />
3533 RB6285 448 1347 3304945 3303599 S-adenosyl-L-homocysteine hydrolase 3.3.1.1 4.8 - -1.52 0.02 1<br />
7190 RB12905 619 1860 6950939 6949080 acetolactate synthase, large subunit, biosynthetic type 5.6 - -1.18 0.30 25<br />
179 RB314 309 930 156968 157897 malonyl CoA-acyl carrier protein transacylase fabD 2.3.1.39 4.4 + -2.18 0.07 3<br />
181 RB318 81 246 158928 159173 Acyl carrier protein acpP 3.7 + -1.22 0.12 10<br />
2733 RB4876 397 1194 2506214 2505021 delta 9 acyl-lipid fatty acid desaturase 1.14.19.- 9.5 - -1.16 0.05 4<br />
145 RB264 64 195 132677 132871 Ribosomal protein S21 12.3 + -1.82 0.12 7<br />
658 RB1233 206 621 629490 628870 30S ribosomal protein S4 rpsD 11.2 - -1.71 0.20 12<br />
3270 RB5801 89 270 3039583 3039852 Ribosomal protein S15 10.6 + -1.48 0.13 9<br />
3618 RB6425 50 153 3380727 3380879 50S ribosomal protein L36 rpl36 11.9 + -1.35 0.15 11<br />
3822 RB6770 81 246 3599862 3599617 Ribosomal protein L27 11.4 - -1.17 0.31 27<br />
3959 RB7022 90 273 3735291 3735563 Ribosomal protein S20 12.3 + -2.08 0.17 8<br />
4014 RB7115 118 357 3779618 3779262 Ribosomal protein L20 12.2 - -1.65 0.21 13<br />
4016 RB7117 181 546 3780322 3779777 Ribosomal protein L35 11.4 - -1.99 0.14 7<br />
4413 RB7818 121 366 4185730 4186095 Ribosomal protein S12/S23 11.8 + -1.68 0.10 6<br />
4414 RB7820 157 474 4186170 4186643 Ribosomal protein S7 10.3 + -1.76 0.06 4<br />
4423 RB7829 108 327 4192031 4192357 Ribosomal protein S10 10.3 + -2.14 0.12 6
4425 RB7833 228 687 4192692 4193378 Ribosomal protein L3 11 + -1.81 0.16 9<br />
4426 RB7834 222 669 4193390 4194058 Ribosomal protein L4/L1e 10.7 + -2.94 0.06 2<br />
4427 RB7836 107 324 4194060 4194383 Ribosomal L23 protein 10.8 + -2.78 0.09 3<br />
4428 RB7837 286 861 4194481 4195341 Ribosomal protein L2 11.8 + -2.68 0.15 6<br />
4429 RB7838 89 270 4195376 4195645 Ribosomal protein S19/S15 10.8 + -2.48 0.05 2<br />
4430 RB7839 119 360 4195706 4196065 Ribosomal protein L22/L17 11 + -2.44 0.11 4<br />
4431 RB7840 236 711 4196161 4196871 30S ribosomal protein S3 rpsC 10.4 + -2.58 0.12 5<br />
4432 RB7841 138 417 4196810 4197226 Ribosomal protein L16 11.1 + -2.77 0.27 10<br />
4434 RB7849 108 327 4197700 4198026 Ribosomal protein S17 10 + -3.32 0.16 5<br />
4435 RB7850 122 369 4198081 4198449 Ribosomal protein L14b/L23e 11 + -3.17 0.12 4<br />
4437 RB7852 196 591 4198935 4199525 50S ribosomal protein L5 rplE 10.4 + -2.01 0.06 3<br />
4438 RB7854 61 186 4199624 4199809 Ribosomal protein S14 11.8 + -3.32 0.19 6<br />
4440 RB7856 181 546 4200306 4200851 50S ribosomal protein L6 rplF 10 + -2.95 0.35 12<br />
4442 RB7859 177 534 4201351 4201884 Ribosomal protein S5 10.6 + -2.47 0.13 5<br />
4463 RB7899 141 426 4220226 4220651 50S ribosomal protein L11 rplK 9.6 + -1.61 0.11 7<br />
4750 RB8459 55 168 4518496 4518663 30S ribosomal protein S18 rpsR 10.5 + -2.53 0.20 8<br />
4824 RB8580 113 342 4577393 4577734 sigma-54 modulation protein 5.7 + -1.65 0.10 6<br />
4892 RB8725 54 165 4638301 4638465 50S ribosomal protein L33 rpmG 10.6 + -1.79 0.13 7<br />
5534 RB9922 190 573 5345441 5344869 Peptidyl-tRNA hydrolase pth 3.1.1.29 8.6 - -1.22 0.18 15<br />
5651 RB10134 104 315 5469243 5468929 Ribosomal protein L21 10 - -1.39 0.10 7<br />
5830 RB10468 73 222 5677835 5677614 Translation initiation factor IF-1 infA 10.3 - -1.36 0.18 14<br />
5831 RB10469 137 414 5678261 5677848 30S ribosomal protein S9 rpsI 11.1 - -1.41 0.16 11<br />
5832 RB10471 151 456 5678759 5678304 Ribosomal protein L13 10 - -2.27 0.02 1<br />
5918 RB10640 326 981 5774380 5775360 Elongation factor Ts tsf 4.6 + -1.15 0.14 12<br />
6055 RB10880 477 1434 5907340 5905907 2-methylthioadenine synthetase 4.4 - -1.20 0.12 10<br />
7029 RB12623 127 384 6796904 6797287 Ribosomal protein S13 10.8 + -2.12 0.15 7<br />
7030 RB12625 127 384 6797354 6797737 Ribosomal protein S11 11.4 + -1.56 0.11 7<br />
7032 RB12628 210 633 6798878 6799510 50S ribosomal protein L17 rplQ 10.2 + -1.03 0.18 17<br />
7143 RB12821 117 354 6916558 6916205 Ribosomal protein L19 11.1 - -1.41 0.19 13<br />
7144 RB12823 242 729 6917306 6916578 tRNA (guanine-N1-)-methyltransferase 2.1.1.31 4.9 - -2.21 0.24 11<br />
7145 RB12824 146 441 6917743 6917303 Ribosomal protein S16 5.3 - -1.07 0.15 14<br />
7154 RB12839 225 678 6921934 6922611 Ribosomal protein L1 9.8 + -1.36 0.15 11<br />
4610 RB8189 931 2796 4382134 4379339 ATP-dependent RNA helicase DeaD 5 - -1.64 0.05 3<br />
1030 RB1964 500 1503 1034475 1035977 hypothetical protein 4.7 + -1.35 0.10 7<br />
2610 RB4681 65 198 2391934 2392131 cold shock protein cspA 4.7 + -1.86 0.36 19<br />
3662 RB6491 526 1581 3438676 3437096 RNA polymerase sigma-54 factor rpoN 4.6 - -1.37 0.09 6<br />
5823 RB10458 206 621 5671130 5670510 Rnpc2-prov protein 10.3 - -1.51 0.10 7<br />
7031 RB12626 331 996 6797797 6798792 DNA-directed RNA polymerase alpha chain rpoA 2.7.7.6 4.9 + -1.83 0.08 4<br />
3605 RB6403 304 915 3369939 3370853 hypothetical protein 5.4 + -1.47 0.10 7<br />
3528 RB6276 105 318 3297504 3297187 Histone-like bacterial DNA-binding protein 10.4 - -2.19 0.25 11<br />
5531 RB9917 169 510 5344370 5343861 Single-strand binding protein/Primosomal replication protein n ssb 5.3 - -2.32 0.14 6<br />
2400 RB4333 361 1086 2230615 2231700 glycosyl transferase, group 1 family protein 6.4 + -1.12 0.18 16<br />
3620 RB6428 164 495 3382816 3382322 conserved hypothetical protein, secreted 6.5 - -1.20 0.14 12<br />
5812 RB10434 379 1140 5652902 5651763 glycosyl transferase, group 1 family protein 9.9 - -1.32 0.05 4<br />
5813 RB10436 399 1200 5654064 5652865 glycosyl transferase, group 1 family protein 9.6 - -1.47 0.14 9<br />
3646 RB6466 640 1923 3416688 3418610 GTP-binding protein TypA/BipA typA 5.1 + -1.13 0.13 11<br />
7146 RB12825 491 1476 6919309 6917834 signal recognition particle protein ffh 9.8 - -2.03 0.11 5<br />
1609 RB2970 261 786 1530415 1531200 Phosphatidylethanolamine N-methyltransferase pmtA 2.1.1.17 6.6 + -1.24 0.13 11<br />
7140 RB12816 257 774 6914710 6915483 glutamine amidotransferase or related peptidase 4.5 + -1.09 0.16 15<br />
594 RB1129 895 2688 583271 580584 conserved hypothetical protein 5.8 - -1.27 0.06 5<br />
6086 RB10934 68 207 5934135 5934341 CsbD-like 4.3 + -1.47 0.08 6<br />
4825 RB8584 159 480 4577819 4578298 Nitrogen regulatory IIA protein ptsN 2.7.1.69 4.4 + -1.15 0.10 9<br />
2 RB2 59 180 182 3 secreted protein 3.7 - -1.15 0.23 20<br />
182 RB319 95 288 159334 159621 hypothetical protein 10.2 + -1.42 0.05 3<br />
415 RB778 413 1242 418773 417532 secreted protein containing DUF1559 7 - -1.41 0.13 9<br />
581 RB1097 404 1215 570281 569067 membrane protein 5.3 - -1.93 0.14 7<br />
582 RB1100 166 501 570778 570278 capsule biosynthesis protein CapC capC 4.9 - -2.09 0.19 9<br />
583 RB1103 441 1326 572105 570780 capsule biosynthesis protein capB 6.3 - -1.96 0.25 13<br />
839 RB1563 136 411 798934 799344 hypothetical protein 10.2 + -1.22 0.07 6<br />
852 RB1588 151 456 817362 817817 ACP-like protein 5 + -1.15 0.22 19<br />
1130 RB2150 93 282 1138311 1138030 hypothetical protein 4.4 - -2.47 0.09 4<br />
1992 RB3644 127 384 1895184 1894801 hypothetical protein 4.9 - -1.09 0.06 6<br />
2312 RB4194 53 162 2156685 2156524 hypothetical protein 11.4 - -1.10 0.14 13<br />
2401 RB4336 372 1119 2231697 2232815 conserved hypothetical protein 5.6 + -1.07 0.25 24<br />
2481 RB4464 223 672 2292075 2291404 secreted protein 5.1 - -1.16 0.03 3<br />
2500 RB4497 49 150 2302950 2303099 hypothetical protein 10.3 + -1.12 0.03 3<br />
2596 RB4657 123 372 2381865 2382236 hypothetical protein 12.2 + -1.05 0.28 26<br />
2781 RB4951 95 288 2547498 2547785 hypothetical protein 12.1 + -1.86 0.14 7<br />
2782 RB4954 166 501 2547861 2548361 secreted protein 10.2 + -1.91 0.15 8<br />
3045 RB5409 97 294 2794195 2794488 hypothetical protein 12.7 + -2.26 0.04 2<br />
3046 RB5411 35 108 2794516 2794623 hypothetical protein 10.5 + -1.24 0.06 5<br />
3049 RB5415 62 189 2798737 2798925 hypothetical protein 12.3 + -1.04 0.09 9<br />
3239 RB5745 130 393 3001234 3000842 hypothetical protein 10.7 - -2.34 0.26 11<br />
3271 RB5802 156 471 3039864 3040334 hypothetical protein 10.1 + -1.57 0.06 4<br />
3588 RB6379 153 462 3353122 3352661 hypothetical protein 5.8 - -1.37 0.12 8<br />
3617 RB6423 45 138 3380603 3380740 hypothetical protein 5.5 + -1.08 0.17 16<br />
3621 RB6429 61 186 3382998 3382813 hypothetical protein 12.1 - -1.03 0.10 10<br />
3643 RB6462 90 273 3414908 3415180 hypothetical protein 9.9 + -1.06 0.00 0<br />
3701 RB6553 74 225 3479627 3479403 hypothetical protein 12.1 - -1.22 0.07 6<br />
4015 RB7116 59 180 3779586 3779765 hypothetical protein 11.7 + -1.64 0.11 7<br />
4063 RB7203 147 444 3819614 3820057 hypothetical protein 4.6 + -1.24 0.20 16<br />
4092 RB7246 50 153 3850393 3850545 hypothetical protein 9.7 + -2.04 0.15 7<br />
4131 RB7313 51 156 3892097 3891942 hypothetical protein 10.5 - -1.00 0.07 7<br />
4200 RB7455 838 2517 3999277 3996761 conserved hypothetical protein containing YTV domains 9 - -1.72 0.30 18<br />
4314 RB7646 62 189 4111735 4111923 hypothetical protein 10.5 + -1.89 0.19 10<br />
4315 RB7647 73 222 4111931 4112152 hypothetical protein 7.4 + -2.13 0.09 4<br />
4327 RB7667 38 117 4117097 4116981 hypothetical protein 6.7 - -2.00 0.14 7<br />
4424 RB7832 50 153 4192499 4192651 hypothetical protein 13 + -2.24 0.08 4<br />
4461 RB7897 152 459 4218945 4219403 Protein secE/sec61-gamma protein 9.6 + -1.67 0.06 3<br />
4589 RB8144 143 432 4354622 4354191 hypothetical protein 10.1 - -2.22 0.10 4<br />
4698 RB8356 69 210 4459802 4460011 hypothetical protein 12.3 + -1.49 0.16 11<br />
4749 RB8457 113 342 4518458 4518117 hypothetical protein 11.5 - -1.04 0.05 5<br />
4818 RB8565 305 918 4573365 4572448 Chain A, 1.8 A Crystal Structure Of Ioli Protein With A Binding Zinc Atom 4.3 - -1.57 0.14 9<br />
4827 RB8590 107 324 4578825 4579148 hypothetical protein 10.4 + -1.28 0.17 13<br />
4830 RB8594 41 126 4581645 4581770 hypothetical protein 9.2 + -1.02 0.19 19<br />
4861 RB8669 37 114 4614557 4614670 hypothetical protein 11.5 + -1.72 0.26 15<br />
5226 RB9356 129 390 5012771 5012382 protein containing DUF1579 9.2 - -1.90 0.23 12<br />
5280 RB9460 79 240 5078860 5078621 hypothetical protein 10.3 - -1.92 0.21 11<br />
5694 RB10209 110 333 5515135 5515467 H+-transporting two-sector ATPase, C subunit 3.6.3.14 10.2 + -1.35 0.19 14<br />
5810 RB10431 323 972 5650397 5649426 conserved hypothetical protein 10.1 - -1.90 0.12 6<br />
5855 RB10521 53 162 5708182 5708343 hypothetical protein 9.1 + -1.01 0.19 19<br />
5887 RB10581 384 1155 5739353 5740507 secreted protein containing DUF1559 6.2 + -1.11 0.24 21<br />
6084 RB10931 54 165 5933742 5933906 hypothetical protein 4.2 + -1.35 0.21 16<br />
6085 RB10932 65 198 5933872 5934069 secreted protein 3.8 + -1.56 0.14 9<br />
6094 RB10948 153 462 5938575 5939036 conserved hypothetical protein, secreted 4.2 + -1.89 0.09 5<br />
7055 RB12664 234 705 6817708 6818412 conserved hypothetical protein, secreted 6.7 + -1.02 0.02 2<br />
7058 RB12669 473 1422 6821952 6823373 membrane protein 9.6 + -1.80 0.05 3<br />
7108 RB12760 50 153 6876908 6877060 conserved hypothetical protein 7.3 + -1.05 0.00 0<br />
7109 RB12761 54 165 6877026 6877190 conserved hypothetical protein 12 + -1.23 0.18 15<br />
7141 RB12818 163 492 6915989 6915498 conserved hypothetical protein 11.7 - -1.89 0.08 4<br />
7153 RB12837 65 198 6921732 6921929 conserved hypothetical protein 9.8 + -2.31 0.19 8<br />
300min ID Locus AA Nuc Product Gene EC IEP Strand Ratio Stdev %<br />
2757 RB4913 109 330 2527319 2527648 ATP synthase C chain atpE 3.6.3.14 6.3 + -1.03 0.19 18<br />
3570 RB6350 832 2499 3333612 3336110 Cytochrome C oxidase, mono-heme subunit/FixO 6 + -1.20 0.25 21<br />
3649 RB6470 701 2106 3420270 3422375 Abortive infection protein 5.9 + -1.37 0.09 7<br />
6105 RB10968 457 1374 5945417 5944044 Succinate-semialdehyde dehydrogenase [NADP+] ssdA 1.2.1.16 4.4 - -1.02 0.17 17<br />
3418 RB6065 1234 3705 3185756 3182052 chromosome segregation SMC protein 5.5 - -1.05 0.21 20<br />
238 RB410 342 1029 219986 218958 anthranilate phosphoribosyltransferase trpD 2.4.2.18 5.1 - -1.06 0.20 19<br />
1967 RB3603 344 1035 1875461 1874427 secreted protein 4.6 - -2.00 0.18 9<br />
1984 RB3627 280 843 1888482 1887640 Transglutaminase-like domain 4.5 - -1.09 0.04 4<br />
2359 RB4269 282 849 2185049 2184201 glutamic acid specific endopeptidase, GSE=serine endopeptidase 5.6 - -2.04 0.37 18
2432 RB4386 349 1050 2259814 2258765 CYSTEINE SYNTHASE A cysK 2.5.1.47 5.6 - -1.52 0.09 6<br />
3188 RB5653 500 1503 2948867 2947365 NADH-glutamate synthase small chain 5 - -1.23 0.10 8<br />
4459 RB7894 398 1197 4217484 4218680 translation elongation factor EF-Tu 5.2 + -2.68 0.03 1<br />
4534 RB8038 1381 4146 4298294 4302439 Hydantoinase/oxoprolinase 5.3 + -1.28 0.06 5<br />
6865 RB12327 686 2061 6633918 6631858 TGF-beta receptor, type I/II extracellular region 4.5 - -1.89 0.20 11<br />
2192 RB3967 544 1635 2053237 2054871 sodium:proline/pantothenate symporter (proline/pantothenate permease) 9.4 + -1.23 0.20 16<br />
571 RB1074 353 1062 558194 559255 Bacterial inner-membrane translocator 9.2 + -1.39 0.05 3<br />
1905 RB3496 511 1536 1808754 1810289 ribose ABC transporter, ATP-binding protein rbsA 3.6.3.17 5.4 + -1.24 0.07 6<br />
4609 RB8188 259 780 4378573 4379352 hydroxypyruvate isomerase hyi 5.3.1.22 4.4 + -1.16 0.12 11<br />
181 RB318 81 246 158928 159173 Acyl carrier protein acpP 3.7 + -1.03 0.09 9<br />
2733 RB4876 397 1194 2506214 2505021 delta 9 acyl-lipid fatty acid desaturase 1.14.19.- 9.5 - -1.51 0.06 4<br />
5006 RB8921 393 1182 4751930 4750749 conserved hypothetical protein, membrane 9.9 - -1.13 0.19 17<br />
145 RB264 64 195 132677 132871 Ribosomal protein S21 12.3 + -1.44 0.08 5<br />
658 RB1233 206 621 629490 628870 30S ribosomal protein S4 rpsD 11.2 - -1.09 0.03 3<br />
3959 RB7022 90 273 3735291 3735563 Ribosomal protein S20 12.3 + -1.44 0.14 9<br />
4016 RB7117 181 546 3780322 3779777 Ribosomal protein L35 11.4 - -1.68 0.05 3<br />
4413 RB7818 121 366 4185730 4186095 Ribosomal protein S12/S23 11.8 + -1.49 0.07 5<br />
4414 RB7820 157 474 4186170 4186643 Ribosomal protein S7 10.3 + -1.66 0.14 8<br />
4423 RB7829 108 327 4192031 4192357 Ribosomal protein S10 10.3 + -1.89 0.18 10<br />
4424 RB7832 50 153 4192499 4192651 hypothetical protein 13 + -1.92 0.08 4<br />
4425 RB7833 228 687 4192692 4193378 Ribosomal protein L3 11 + -1.31 0.03 2<br />
4426 RB7834 222 669 4193390 4194058 Ribosomal protein L4/L1e 10.7 + -2.60 0.03 1<br />
4427 RB7836 107 324 4194060 4194383 Ribosomal L23 protein 10.8 + -2.24 0.09 4<br />
4428 RB7837 286 861 4194481 4195341 Ribosomal protein L2 11.8 + -1.84 0.13 7<br />
4429 RB7838 89 270 4195376 4195645 Ribosomal protein S19/S15 10.8 + -1.79 0.09 5<br />
4430 RB7839 119 360 4195706 4196065 Ribosomal protein L22/L17 11 + -1.60 0.08 5<br />
4431 RB7840 236 711 4196161 4196871 30S ribosomal protein S3 rpsC 10.4 + -1.72 0.04 2<br />
4432 RB7841 138 417 4196810 4197226 Ribosomal protein L16 11.1 + -1.56 0.14 9<br />
4434 RB7849 108 327 4197700 4198026 Ribosomal protein S17 10 + -2.33 0.09 4<br />
4435 RB7850 122 369 4198081 4198449 Ribosomal protein L14b/L23e 11 + -2.31 0.01 0<br />
4436 RB7851 115 348 4198529 4198876 50S ribosomal protein L24 rplX 10.5 + -2.06 0.08 4<br />
4437 RB7852 196 591 4198935 4199525 50S ribosomal protein L5 rplE 10.4 + -2.06 0.06 3<br />
4438 RB7854 61 186 4199624 4199809 Ribosomal protein S14 11.8 + -2.29 0.06 3<br />
4439 RB7855 133 402 4199839 4200240 Ribosomal protein S8 10.4 + -1.91 0.17 9<br />
4440 RB7856 181 546 4200306 4200851 50S ribosomal protein L6 rplF 10 + -2.10 0.18 9<br />
4441 RB7857 149 450 4200854 4201303 Ribosomal protein L18P/L5E 11.6 + -1.80 0.03 2<br />
4442 RB7859 177 534 4201351 4201884 Ribosomal protein S5 10.6 + -1.52 0.10 7<br />
4443 RB7863 164 495 4202048 4202542 Ribosomal protein L15 11.2 + -1.05 0.22 20<br />
4750 RB8459 55 168 4518496 4518663 30S ribosomal protein S18 rpsR 10.5 + -1.11 0.07 7<br />
5534 RB9922 190 573 5345441 5344869 Peptidyl-tRNA hydrolase pth 3.1.1.29 8.6 - -1.29 0.09 7<br />
5651 RB10134 104 315 5469243 5468929 Ribosomal protein L21 10 - -1.36 0.27 20<br />
5832 RB10471 151 456 5678759 5678304 Ribosomal protein L13 10 - -1.34 0.08 6<br />
7029 RB12623 127 384 6796904 6797287 Ribosomal protein S13 10.8 + -1.56 0.09 6<br />
7030 RB12625 127 384 6797354 6797737 Ribosomal protein S11 11.4 + -1.58 0.08 5<br />
7032 RB12628 210 633 6798878 6799510 50S ribosomal protein L17 rplQ 10.2 + -1.22 0.03 3<br />
7145 RB12824 146 441 6917743 6917303 Ribosomal protein S16 5.3 - -1.08 0.03 3<br />
7154 RB12839 225 678 6921934 6922611 Ribosomal protein L1 9.8 + -1.72 0.06 4<br />
7156 RB12842 140 423 6923401 6923823 Ribosomal protein L7/L12 4.3 + -1.52 0.08 5<br />
4610 RB8189 931 2796 4382134 4379339 ATP-dependent RNA helicase DeaD 5 - -1.77 0.16 9<br />
6709 RB12047 485 1458 6497993 6499450 ATP-dependent RNA helicase rhlE rhlE 10.6 + -1.60 0.15 10<br />
2610 RB4681 65 198 2391934 2392131 cold shock protein cspA 4.7 + -1.65 0.09 6<br />
3662 RB6491 526 1581 3438676 3437096 RNA polymerase sigma-54 factor rpoN 4.6 - -1.26 0.11 9<br />
5823 RB10458 206 621 5671130 5670510 Rnpc2-prov protein 10.3 - -1.47 0.08 5<br />
6819 RB12251 567 1704 6599262 6597559 DNA-directed RNA polymerase specialized sigma factor 9.4 - -1.57 0.01 1<br />
7031 RB12626 331 996 6797797 6798792 DNA-directed RNA polymerase alpha chain rpoA 2.7.7.6 4.9 + -1.02 0.11 11<br />
3605 RB6403 304 915 3369939 3370853 hypothetical protein 5.4 + -1.84 0.06 3<br />
3528 RB6276 105 318 3297504 3297187 Histone-like bacterial DNA-binding protein 10.4 - -1.10 0.06 5<br />
2400 RB4333 361 1086 2230615 2231700 glycosyl transferase, group 1 family protein 6.4 + -1.29 0.08 6<br />
2479 RB4461 473 1422 2291114 2289693 MscS Mechanosensitive ion channel 4.5 - -1.38 0.08 6<br />
3738 RB6624 503 1512 3520589 3522100 Bacterial sugar transferase 2.7.8.6 7.7 + -1.08 0.09 9<br />
5813 RB10436 399 1200 5654064 5652865 glycosyl transferase, group 1 family protein 9.6 - -1.09 0.16 14<br />
5814 RB10439 526 1581 5655714 5654134 Bacterial sugar transferase 2.7.8.6 10.1 - -1.16 0.15 13<br />
3479 RB6180 100 303 3240284 3240586 Bacterial sec-independent translocation protein mttA/Hcf106 5.5 + -1.01 0.20 20<br />
6766 RB12160 108 327 6563104 6562778 thioredoxin trxA 4 - -1.12 0.09 8<br />
374 RB684 653 1962 368557 366596 Arylsulfatase precursor atsA 3.1.6.1 5.5 - -1.04 0.14 14<br />
2185 RB3956 489 1470 2050223 2048754 SULFATASE 5.5 - -1.06 0.04 4<br />
5754 RB10326 588 1767 5580833 5582599 sulfate permease family protein 6 + -1.18 0.07 6<br />
537 RB990 500 1503 520003 521505 Major facilitator superfamily MFS_1 9.7 + -1.11 0.12 11<br />
4107 RB7276 916 2751 3866177 3868927 peptide synthetase 5 + -1.08 0.14 13<br />
1332 RB2479 273 822 1296683 1295862 conserved hypothetical protein 5.5 - -1.13 0.17 15<br />
1609 RB2970 261 786 1530415 1531200 Phosphatidylethanolamine N-methyltransferase pmtA 2.1.1.17 6.6 + -1.30 0.04 3<br />
359 RB658 412 1239 356228 354990 transport protein 5.2 - -1.68 0.06 3<br />
2427 RB4377 395 1188 2255017 2253830 transport protein 5.7 - -1.11 0.06 5<br />
2476 RB4458 342 1029 2287089 2286061 Protein of unknown function, UPF0118 6.4 - -1.13 0.12 10<br />
4255 RB7557 327 984 4058936 4059919 von Willebrand factor type A domain protein 4.9 + -1.09 0.05 5<br />
4619 RB8208 412 1239 4387234 4388472 chlorohydrolase family protein 5.9 + -1.15 0.13 12<br />
5487 RB9840 869 2610 5299200 5301809 DNA internalization-related competence protein ComEC/ 8.6 + -1.27 0.19 15<br />
6115 RB10987 294 885 5954713 5955597 Phenazine biosynthesis PhzC/PhzF protein 4.6 + -1.04 0.08 8<br />
6639 RB11920 249 750 6418443 6417694 Protein of unknown function Zn-binding 9.1 - -1.02 0.10 10<br />
594 RB1129 895 2688 583271 580584 conserved hypothetical protein 5.8 - -1.53 0.13 9<br />
2337 RB4229 419 1260 2167854 2166595 conserved hypothetical protein 4.6 - -1.22 0.06 5<br />
5039 RB8988 303 912 4790886 4791797 conserved hypothetical protein 6.1 + -1.02 0.05 5<br />
5277 RB9448 115 348 5076267 5076614 conserved hypothetical membrane protein 7.4 + -1.01 0.16 15<br />
3918 RB6945 589 1770 3686749 3688518 ADENYLATE CYCLASE TRANSMEMBRANE PROTEIN 9.8 + -1.08 0.18 16<br />
6279 RB11286 131 396 6117961 6118356 TspO/MBR-related protein 9.8 + -1.12 0.21 19<br />
37 RB61 58 177 25378 25554 hypothetical protein 9.6 + -1.44 0.09 6<br />
56 RB95 157 474 40090 39617 conserved hypothetical protein, membrane 9.6 - -1.02 0.13 12<br />
182 RB319 95 288 159334 159621 hypothetical protein 10.2 + -1.26 0.08 6<br />
193 RB337 97 294 169241 169534 hypothetical protein 12.5 + -1.10 0.15 14<br />
242 RB418 363 1092 222222 223313 conserved hypothetical protein, membrane 10.3 + -1.25 0.09 7<br />
250 RB434 65 198 231791 231988 hypothetical protein 7.4 + -1.02 0.20 20<br />
402 RB752 1026 3081 399966 403046 hypothetical protein 4.8 + -1.13 0.11 10<br />
409 RB767 311 936 411420 412355 conserved hypothetical protein, secreted 5.7 + -1.65 0.12 7<br />
415 RB778 413 1242 418773 417532 secreted protein containing DUF1559 7 - -1.95 0.09 5<br />
449 RB825 117 354 434184 433831 hypothetical protein 7.8 - -1.12 0.21 19<br />
516 RB951 234 705 502459 501755 protein containing DUF1596 12.3 - -1.12 0.27 24<br />
581 RB1097 404 1215 570281 569067 membrane protein 5.3 - -1.80 0.07 4<br />
582 RB1100 166 501 570778 570278 capsule biosynthesis protein CapC capC 4.9 - -2.91 0.07 3<br />
583 RB1103 441 1326 572105 570780 capsule biosynthesis protein capB 6.3 - -2.52 0.33 13<br />
849 RB1581 582 1749 814265 812517 membrane protein 6.7 - -1.54 0.15 10<br />
883 RB1644 123 372 845824 845453 secreted protein 9.7 - -2.32 0.10 4<br />
1074 RB2051 367 1104 1079177 1080280 secreted protein 4.3 + -1.06 0.15 14<br />
1105 RB2105 470 1413 1114012 1112600 membrane protein 9.6 - -1.14 0.06 6<br />
1130 RB2150 93 282 1138311 1138030 hypothetical protein 4.4 - -1.20 0.04 4<br />
1220 RB2306 41 126 1206782 1206907 hypothetical protein 9 + -1.03 0.08 8<br />
1285 RB2400 66 201 1248699 1248499 hypothetical protein 12.1 - -1.01 0.09 9<br />
1626 RB2993 97 294 1550784 1550491 hypothetical protein 5.3 - -1.02 0.15 14<br />
1627 RB2995 95 288 1551052 1550765 hypothetical protein 6.5 - -1.23 0.15 12<br />
1649 RB3042 291 876 1575622 1574747 secreted protein 6 - -1.24 0.10 8<br />
1776 RB3277 221 666 1686552 1685887 hypothetical protein 10.4 - -1.21 0.10 9<br />
1825 RB3362 87 264 1728546 1728283 hypothetical protein 11.9 - -1.19 0.18 15<br />
1828 RB3366 78 237 1730380 1730616 hypothetical protein 5.6 + -1.24 0.23 19<br />
1844 RB3394 36 111 1752044 1752154 hypothetical protein 10.5 + -1.29 0.14 11<br />
1847 RB3399 65 198 1753650 1753453 hypothetical protein 9.7 - -1.28 0.20 16<br />
1854 RB3407 89 270 1759935 1759666 hypothetical protein 10.5 - -1.35 0.14 10<br />
1950 RB3575 152 459 1858779 1859237 membrane protein 10.1 + -1.38 0.07 5<br />
1992 RB3644 127 384 1895184 1894801 hypothetical protein 4.9 - -1.50 0.03 2<br />
2017 RB3675 742 2229 1904552 1906780 secreted protein 8.4 + -1.21 0.03 3<br />
2025 RB3688 53 162 1910629 1910468 hypothetical protein 9.3 - -1.27 0.10 8<br />
2139 RB3880 82 249 2006953 2006705 hypothetical protein 10.7 - -1.11 0.14 13<br />
2146 RB3890 125 378 2011554 2011177 hypothetical protein 4.4 - -1.04 0.16 15<br />
2166 RB3925 50 153 2033534 2033382 hypothetical protein 10.2 - -1.13 0.10 8<br />
2184 RB3953 857 2574 2048784 2046211 hypothetical protein 5.2 - -1.44 0.06 4
2198 RB3975 204 615 2056726 2057340 hypothetical protein 12.3 + -1.22 0.09 8<br />
2200 RB3981 161 486 2058000 2058485 hypothetical protein 4.1 + -1.23 0.19 15<br />
2210 RB3994 191 576 2061291 2061866 hypothetical protein 4.1 + -1.04 0.20 19<br />
2254 RB4084 119 360 2104677 2105036 hypothetical protein 10 + -1.04 0.25 24<br />
2260 RB4097 733 2202 2108244 2110445 conserved hypothetical protein 6.2 + -1.61 0.04 2<br />
2287 RB4145 90 273 2131933 2131661 hypothetical protein 12 - -1.27 0.04 3<br />
2356 RB4264 120 363 2183157 2182795 membrane protein 10.7 - -1.05 0.10 10<br />
2357 RB4266 54 165 2183250 2183414 hypothetical protein 11.2 + -1.11 0.15 13<br />
2368 RB4282 291 876 2199894 2199019 conserved hypothetical protein 5.3 - -1.20 0.15 13<br />
2406 RB4343 144 435 2235597 2235163 hypothetical protein 11.1 - -1.02 0.14 13<br />
2407 RB4347 156 471 2235735 2236205 conserved hypothetical protein 4.7 + -1.06 0.08 8<br />
2416 RB4358 123 372 2239474 2239103 hypothetical protein 6.5 - -1.19 0.18 16<br />
2424 RB4373 109 330 2242863 2242534 hypothetical protein 4.8 - -1.30 0.09 7<br />
2428 RB4378 50 153 2255166 2255014 hypothetical protein 4.5 - -1.05 0.13 12<br />
2444 RB4402 53 162 2265340 2265501 hypothetical protein 10.4 + -1.09 0.21 19<br />
2481 RB4464 223 672 2292075 2291404 secreted protein 5.1 - -1.05 0.10 9<br />
2490 RB4477 69 210 2295940 2296149 hypothetical protein 10.6 + -1.23 0.14 12<br />
2491 RB4478 172 519 2296122 2296640 membrane protein 7.3 + -1.02 0.12 11<br />
2503 RB4500 87 264 2303645 2303908 conserved hypothetical protein, secreted 3.5 + -1.20 0.03 2<br />
2504 RB4502 59 180 2303992 2304171 Protein of unknown function, UPF0057 9.1 + -1.47 0.05 3<br />
2781 RB4951 95 288 2547498 2547785 hypothetical protein 12.1 + -1.45 0.13 9<br />
2782 RB4954 166 501 2547861 2548361 secreted protein 10.2 + -1.17 0.07 6<br />
2947 RB5229 244 735 2695138 2695872 membrane protein 9.6 + -1.25 0.23 18<br />
2964 RB5262 95 288 2713742 2714029 membrane protein 6.3 + -1.28 0.07 5<br />
3239 RB5745 130 393 3001234 3000842 hypothetical protein 10.7 - -1.73 0.07 4<br />
3271 RB5802 156 471 3039864 3040334 hypothetical protein 10.1 + -1.57 0.04 3<br />
3388 RB6009 122 369 3149941 3149573 hypothetical protein 4.3 - -1.30 0.10 8<br />
3430 RB6092 361 1086 3192387 3193472 Peptidase M50 3.4.24.- 9.6 + -1.38 0.09 7<br />
3466 RB6158 142 429 3233532 3233104 hypothetical protein 6 - -1.14 0.22 20<br />
3476 RB6174 69 210 3239734 3239525 hypothetical protein 10.6 - -1.12 0.12 11<br />
3527 RB6274 59 180 3297130 3296951 hypothetical protein 12 - -1.18 0.08 7<br />
3571 RB6351 58 177 3336171 3336347 hypothetical protein 4.2 + -1.42 0.05 4<br />
3588 RB6379 153 462 3353122 3352661 hypothetical protein 5.8 - -1.10 0.09 8<br />
3617 RB6423 45 138 3380603 3380740 hypothetical protein 5.5 + -1.05 0.08 8<br />
3625 RB6434 48 147 3388682 3388536 hypothetical protein 7.4 - -1.03 0.15 14<br />
3644 RB6463 189 570 3415155 3415724 conserved hypothetical protein, membrane 10.2 + -1.17 0.08 7<br />
3701 RB6553 74 225 3479627 3479403 hypothetical protein 12.1 - -1.07 0.09 9<br />
3709 RB6567 37 114 3489298 3489185 hypothetical protein 7.6 - -1.15 0.07 6<br />
3725 RB6594 295 888 3505472 3506359 hypothetical protein 10.2 + -1.46 0.12 8<br />
3742 RB6632 55 168 3524963 3525130 hypothetical protein 5.6 + -1.09 0.07 7<br />
3744 RB6634 365 1098 3525785 3526882 protein containing DUF1559 5.3 + -1.27 0.08 6<br />
3780 RB6699 47 144 3559781 3559924 hypothetical protein 11.1 + -1.00 0.11 11<br />
3820 RB6766 55 168 3597367 3597534 hypothetical protein 12 + -1.12 0.11 10<br />
3867 RB6849 101 306 3642537 3642232 hypothetical protein 12.8 - -1.09 0.21 19<br />
3971 RB7042 91 276 3743971 3744246 hypothetical protein 10.4 + -1.60 0.36 23<br />
3991 RB7082 119 360 3761766 3761407 membrane protein 9.6 - -1.04 0.14 13<br />
4200 RB7455 838 2517 3999277 3996761 conserved hypothetical protein containing YTV domains 9 - -1.17 0.11 9<br />
4314 RB7646 62 189 4111735 4111923 hypothetical protein 10.5 + -1.72 0.09 5<br />
4315 RB7647 73 222 4111931 4112152 hypothetical protein 7.4 + -1.27 0.18 14<br />
4531 RB8034 147 444 4295344 4295787 membrane protein 10.2 + -1.36 0.11 8<br />
4578 RB8119 142 429 4346427 4346855 hypothetical protein 10.1 + -1.24 0.09 7<br />
4588 RB8143 72 219 4354179 4353961 hypothetical protein 10.3 - -1.63 0.16 10<br />
4589 RB8144 143 432 4354622 4354191 hypothetical protein 10.1 - -1.89 0.16 8<br />
4629 RB8227 59 180 4400365 4400186 hypothetical protein 9.3 - -1.06 0.08 8<br />
4830 RB8594 41 126 4581645 4581770 hypothetical protein 9.2 + -1.28 0.13 10<br />
4843 RB8625 156 471 4599385 4599855 secreted protein 5.5 + -1.18 0.04 4<br />
4971 RB8866 445 1338 4716964 4718301 secreted conserved hypothetical protein 6 + -1.17 0.17 15<br />
5091 RB9101 183 552 4863681 4863130 secreted YceI like family protein 6.5 - -1.02 0.04 4<br />
5124 RB9160 118 357 4900491 4900847 hypothetical protein 10.8 + -1.17 0.23 20<br />
5214 RB9343 59 180 5003789 5003968 hypothetical protein 11.4 + -1.11 0.10 9<br />
5258 RB9417 103 312 5050621 5050932 hypothetical protein 10.5 + -1.66 0.22 13<br />
5280 RB9460 79 240 5078860 5078621 hypothetical protein 10.3 - -1.67 0.15 9<br />
5371 RB9624 357 1074 5181259 5180186 hypothetical protein 5.2 - -1.34 0.22 17<br />
5445 RB9767 97 294 5264348 5264641 hypothetical protein 8.9 + -1.27 0.22 17<br />
5503 RB9872 67 204 5316692 5316895 hypothetical protein 5.4 + -1.11 0.13 12<br />
5644 RB10123 72 219 5463443 5463661 hypothetical protein 10.8 + -1.27 0.25 19<br />
5694 RB10209 110 333 5515135 5515467 H+-transporting two-sector ATPase, C subunit 3.6.3.14 10.2 + -1.60 0.04 3<br />
5809 RB10430 330 993 5649511 5648519 enzyme, C-terminal domain similar to sulfotransferase protein 9.9 - -1.03 0.24 24<br />
5810 RB10431 323 972 5650397 5649426 conserved hypothetical protein 10.1 - -1.95 0.09 5<br />
5900 RB10603 272 819 5758745 5759563 secreted protein 10.3 + -1.15 0.10 8<br />
5933 RB10668 58 177 5796528 5796352 hypothetical protein 9.3 - -1.42 0.09 6<br />
5937 RB10674 58 177 5800080 5799904 hypothetical protein 11.9 - -1.14 0.06 5<br />
5962 RB10712 84 255 5824976 5825230 hypothetical protein 10.2 + -1.55 0.12 8<br />
6098 RB10956 117 354 5940336 5940689 hypothetical protein 4.8 + -1.05 0.06 5<br />
6099 RB10957 99 300 5940708 5941007 conserved hypothetical protein 5.6 + -1.14 0.08 7<br />
6280 RB11287 75 228 6118405 6118632 hypothetical protein 9.1 + -1.29 0.09 7<br />
6319 RB11343 93 282 6154696 6154977 conserved hypothetical protein 12.2 + -1.26 0.26 21<br />
6343 RB11392 148 447 6177208 6177654 conserved hypothetical protein 5 + -1.16 0.09 8<br />
6416 RB11490 181 546 6215558 6216103 conserved hypothetical protein, membrane 10.3 + -1.31 0.26 20<br />
6495 RB11639 63 192 6276931 6277122 conserved hypothetical protein 10.1 + -1.00 0.03 3<br />
6515 RB11671 57 174 6296756 6296929 conserved hypothetical protein 10.3 + -1.24 0.13 10<br />
6539 RB11707 83 252 6312937 6313188 conserved hypothetical protein 9.8 + -1.15 0.11 9<br />
6569 RB11766 129 390 6334355 6334744 conserved hypothetical protein 10.4 + -1.40 0.04 3<br />
6623 RB11886 933 2802 6403682 6400881 protein containing DUF1585 5.1 - -1.22 0.18 14<br />
6708 RB12046 51 156 6497841 6497996 conserved hypothetical protein 11.9 + -1.12 0.01 1<br />
6781 RB12193 36 111 6575977 6575867 conserved hypothetical protein 7.5 - -1.70 0.08 5<br />
6867 RB12329 110 333 6634234 6634566 conserved hypothetical protein, membrane 4 + -1.30 0.06 4<br />
6904 RB12396 57 174 6670870 6670697 conserved hypothetical protein 11.3 - -1.43 0.06 4<br />
6920 RB12426 137 414 6684059 6684472 conserved hypothetical protein, membrane 10.6 + -1.20 0.02 1<br />
6941 RB12454 199 600 6703647 6703048 conserved hypothetical protein 10.3 - -1.28 0.14 11<br />
6996 RB12557 41 126 6765784 6765659 conserved hypothetical protein 9.2 - -1.27 0.16 12<br />
7028 RB12621 85 258 6796861 6796604 conserved hypothetical protein 8.9 - -1.15 0.19 17<br />
7153 RB12837 65 198 6921732 6921929 conserved hypothetical protein 9.8 -1.61 0.09 6<br />
7280 RB13095 229 690 7026753 7027442 conserved hypothetical protein 7.7 -1.09 0.23 21
Cluster 1<br />
Original row UID START STOP heat_10min heat_20min heat_40min heat_60min heat_300min cold_10min cold_20min cold_40min cold_60min cold_300min salt_10min salt_20min salt_40min salt_60min salt_300min<br />
2774 RB2627_Glyceraldehyde 3-phosphate dehydrogenase 1364551 1365579 -1.44 -1.58 -1.3 -1.66 -1.97 -0.42 -0.73 -0.44 -0.5 -0.03 NaN NaN NaN -1.50 -0.94<br />
5947 RB7646_hypothetical protein 4111735 4111923 -1.63 -1.5 -1.26 -1.42 -2.38 -1.6 -1.81 -1.56 -1.79 -1.18 -1.19 -1.24 -0.71 -1.89 -1.72<br />
5948 RB7647_hypothetical protein 4111931 4112152 -1.65 -2.2 -2.99 -2.21 -2.89 0.33 -1.26 -1.13 -0.93 -1.36 -1.51 -1.91 -1.71 -2.13 -1.27<br />
6072 RB7837_Ribosomal protein L2 4194481 4195341 -0.7 -1.25 -1.42 -1.29 -1.3 -0.68 -1.12 -0.83 -0.72 -0.58 -1.87 NaN NaN -2.68 -1.84<br />
283 RB10471_Ribosomal protein L13 5678759 5678304 -1.15 -1.45 -1.83 -1.94 -1.62 -0.82 -0.89 -0.95 -0.69 -0.72 -1.63 -2.13 NaN -2.27 -1.34<br />
864 RB11439_conserved hypothetical protein 6197993 6198274 -2.53 -2.35 -1.91 -1.73 -3.09 -1.21 -0.64 -0.69 -0.98 -1.51 -0.99 -0.34 -0.24 -0.62 0.13<br />
1391 RB12327_TGF-beta receptor, type I/II extracellular region 6633918 6631858 -1.29 -1.69 -1.62 -2.84 -1.81 -0.74 -1.04 -0.78 -0.62 -1.42 -0.79 -0.53 -0.55 -1.18 -1.89<br />
1574 RB12623_Ribosomal protein S13 6796904 6797287 -0.75 -1.35 -1.38 -1.06 -1.08 -0.66 -0.63 -0.58 -0.22 -0.3 -1.19 -1.76 NaN -1.64 -1.56<br />
1575 RB12625_Ribosomal protein S11 6797354 6797737 -0.9 -1.04 -1.23 -1.48 -1.32 -0.68 -0.67 -0.44 -0.35 -0.38 -1.33 -1.76 NaN -2.12 -1.58<br />
1699 RB12818_conserved hypothetical protein 6915989 6915498 -1.82 -1.97 -2.38 -2.23 -3.62 -1.36 -1.17 -1.06 -1.4 -0.93 -0.83 -0.66 -0.12 -1.09 0.09<br />
1701 RB12821_Ribosomal protein L19 6916558 6916205 -0.94 -1.55 -1.29 -1.28 -1.1 -1.52 -1.15 -0.71 -0.86 -0.69 NaN NaN NaN -1.89 -0.52<br />
1703 RB12824_Ribosomal protein S16 6917743 6917303 -0.99 -1.54 -1.46 -1.65 -1.44 -1.42 -1.28 -0.92 -1.25 -0.64 -1.70 NaN NaN -2.21 -1.08<br />
1711 RB12837_conserved hypothetical protein 6921732 6921929 -0.97 -0.82 -0.73 -0.77 -1.45 -0.7 -1.05 -0.97 -1.12 -0.38 -1.64 -1.75 NaN -2.03 -1.61<br />
1712 RB12839_Ribosomal protein L1 6921934 6922611 -1.82 -1.54 -1.33 -1.49 -2.42 -1.6 -1.75 -1.69 -1.69 -0.59 -1.98 -2.24 NaN -2.31 -1.72<br />
1715 RB12842_Ribosomal protein L7/L12 6923401 6923823 -0.88 -1.74 -0.98 -1.03 -1.55 NaN -1.37 -1.52 -1.57 -0.13 NaN NaN NaN NaN -1.52<br />
2182 RB1644_secreted protein 845824 845453 -0.74 -0.76 -2.49 -2.54 -1.52 -0.2 -0.32 -0.04 0.12 -0.21 -1.99 -1.05 -0.27 -0.88 -2.32<br />
2367 RB2_secreted protein 182 3 -2.66 -3.11 -2.51 -3.23 -3.57 -0.82 -0.73 -0.64 -0.85 0.24 -0.96 NaN NaN -1.15 -0.15<br />
2460 RB2150_hypothetical protein 1138311 1138030 -2.06 -1.92 -1.73 -2.08 -2.17 -0.52 -0.78 -0.75 -0.67 -0.03 -1.73 NaN NaN -2.47 -1.20<br />
3082 RB314_malonyl CoA-acyl carrier protein transacylase 156968 157897 -1.91 -2.1 -2.23 -2.09 -2.82 -1.1 -0.97 -1.01 -1.15 -0.57 -1.02 -1.45 -1.11 -2.18 -0.78<br />
3112 RB319_hypothetical protein 159334 159621 -1.56 -1.23 -1.18 -1.37 -1.71 -0.91 -1.49 -1.24 -1.34 -0.88 -0.74 -0.69 -0.49 -1.42 -1.26<br />
3769 RB4194_hypothetical protein 2156685 2156524 -1.35 -1.11 -1.51 -1.86 -1.98 -1.34 -1.09 -0.84 -1.2 -1.1 -0.47 -1.15 -1.21 -1.10 0.24<br />
4036 RB4590_hypothetical protein 2352024 2351800 -0.93 -1.62 -4.03 -3.1 -2.32 -0.57 -1.89 -1.53 -1.19 -1.91 -1.39 -0.25 -0.63 -0.66 -0.18<br />
4084 RB4657_hypothetical protein 2381865 2382236 -1.87 -1.59 -1.61 -1.64 -1.93 -0.75 -1.69 -1.14 -1.12 -1.41 -0.96 -0.59 -0.62 -1.05 -0.26<br />
4100 RB4681_cold shock protein 2391934 2392131 -1.93 -2.75 -2.69 -2.42 -2.85 0.35 -0.13 0.06 -0.5 0.24 -0.81 NaN NaN -1.86 -1.65<br />
4234 RB4876_delta 9 acyl-lipid fatty acid desaturase 2506214 2505021 -1.19 -1.17 -2.67 -2.54 -1.93 -0.34 -0.46 -0.27 -0.09 -0.64 -1.60 -1.25 -1.57 -1.16 -1.51<br />
4285 RB4951_hypothetical protein 2547498 2547785 -1.38 -0.79 -0.64 -0.77 -1.71 -1.25 -1.52 -1.36 -1.45 -0.93 -1.22 -1.11 -0.36 -1.86 -1.45<br />
4293 RB4966_hypothetical protein 2552311 2552057 -0.97 -1.57 -2.67 -2.29 -2.05 0.07 -1.66 -1.43 -1.19 -1.4 -0.74 0.12 -0.29 -0.79 -0.38<br />
4577 RB5409_hypothetical protein 2794195 2794488 -1.98 -2.73 -3.11 -2.8 -3.71 -0.64 -0.78 -1.07 -1.25 -0.8 -1.43 -1.41 -1.83 -2.26 -0.05<br />
4789 RB5745_hypothetical protein 3001234 3000842 -1.89 -2.96 -3.11 -3.11 -2.9 -1.99 -2.4 -1.67 -2.08 -1.21 -1.78 NaN NaN -2.34 -1.73<br />
5097 RB6276_Histone-like bacterial DNA-binding protein 3297504 3297187 -2.04 -2.23 -3.21 -2.79 -2.57 -0.84 -1.3 -0.8 -0.98 -0.7 -1.50 -2.03 -2.82 -2.19 -1.10<br />
5622 RB7116_hypothetical protein 3779586 3779765 -1.37 -1.53 -1.5 -1.59 -2.16 -1.72 -1.6 -1.04 -1.46 -0.98 -0.92 -1.41 NaN -1.64 -0.63<br />
5623 RB7117_Ribosomal protein L35 3780322 3779777 -1.48 -1.85 -1.75 -1.88 -1.77 -0.49 -1.01 -0.65 -0.74 -0.78 -1.21 -1.19 -1.04 -1.99 -1.68<br />
5823 RB7455_conserved hypothetical protein containing YTV domains 3999277 3996761 -1.24 -1.56 -1.43 -1.39 -1.93 -0.6 -0.72 -0.53 -0.51 -0.17 -0.91 NaN NaN -1.72 -1.17<br />
6066 RB7829_Ribosomal protein S10 4192031 4192357 -0.95 -1.07 -1.82 -2.06 -1.55 -0.89 -0.49 -0.46 -0.13 -0.68 -2.05 -1.70 NaN -2.14 -1.89<br />
6068 RB7832_hypothetical protein 4192499 4192651 -1.16 -1.38 -2.2 -1.99 -1.85 -0.36 -0.18 -0.68 -0.06 -0.88 -1.79 -1.67 NaN -2.24 -1.92<br />
6070 RB7834_Ribosomal protein L4/L1e 4193390 4194058 -0.87 -0.98 -1.64 -1.52 -1.47 -0.57 -0.43 -0.52 -0.37 -0.32 -1.86 NaN NaN -2.94 -2.60<br />
6071 RB7836_Ribosomal L23 protein 4194060 4194383 -1.02 -1.27 -2.01 -1.85 -1.94 -0.78 -0.98 -0.7 -0.77 -0.69 -2.36 -2.13 NaN -2.78 -2.24<br />
6073 RB7838_Ribosomal protein S19/S15 4195376 4195645 -1.05 -1.29 -1.05 -1.18 -1.79 -1.81 -1.89 -1.36 -1.37 -0.7 NaN -1.41 NaN -2.48 -1.79<br />
6074 RB7839_Ribosomal protein L22/L17 4195706 4196065 -0.95 -1.21 -1 -1.25 -1.44 -1.45 -1.63 -1.15 -1.19 -0.35 -1.88 NaN NaN -2.44 -1.60<br />
6075 RB7840_30S ribosomal protein S3 4196161 4196871 -0.87 -1.37 -1.38 -1.53 -1.6 -1.84 -2.09 -1.24 -1.45 -0.5 NaN NaN NaN -2.58 -1.72<br />
6076 RB7841_Ribosomal protein L16 4196810 4197226 -0.87 -1.63 -1.02 -1.36 -1.78 -2.59 -2.41 -1.88 -2.14 -0.49 NaN NaN NaN -2.77 -1.56<br />
6078 RB7849_Ribosomal protein S17 4197700 4198026 -1.04 -1.61 -1.35 -1.58 -2.08 -2.94 -2.85 -2.41 -2.38 -0.75 NaN NaN NaN -3.32 -2.33<br />
6080 RB7850_Ribosomal protein L14b/L23e 4198081 4198449 -1.05 -1.35 -1.08 -1.32 -1.99 -2.84 -2.68 -2.15 -2.53 -0.73 NaN NaN NaN -3.17 -2.31<br />
6081 RB7851_50S ribosomal protein L24 4198529 4198876 -0.68 -1.06 -1.15 -1.34 -1.23 NaN -2.68 -2.22 -2.36 -0.46 NaN NaN NaN NaN -2.06<br />
6082 RB7852_50S ribosomal protein L5 4198935 4199525 -0.67 -0.75 -0.53 -0.97 -1.5 -2.06 -1.78 -1.43 -1.97 -0.32 NaN -0.92 -0.68 -2.01 -2.06<br />
6083 RB7854_Ribosomal protein S14 4199624 4199809 -0.98 -1.17 -0.76 -1.17 -2.04 -2.33 -2.24 -1.75 -2.21 -0.24 -2.40 -2.12 NaN -3.32 -2.29<br />
6085 RB7856_50S ribosomal protein L6 4200306 4200851 -1.13 -1.47 -1.06 -1.38 -2.06 -2.13 -1.99 -1.75 -2.05 -0.38 NaN -1.44 NaN -2.95 -2.10<br />
6086 RB7857_Ribosomal protein L18P/L5E 4200854 4201303 -0.92 -1.6 -1.27 -1.49 -1.83 -2.22 -1.66 -1.4 -1.76 -0.64 NaN NaN NaN NaN -1.80<br />
6087 RB7859_Ribosomal protein S5 4201351 4201884 -0.89 -1.21 -0.93 -1.52 -1.67 -1.47 -1.32 -1.06 -1.38 -0.49 -1.36 -1.36 NaN -2.47 -1.52<br />
6101 RB7894_translation elongation factor EF-Tu 4217484 4218680 -1.75 -2.56 -2.77 -2.75 -2.97 -1.95 -2.37 -2.02 -2.38 -1.51 -2.39 NaN NaN -3.10 -2.68<br />
6104 RB7899_50S ribosomal protein L11 4220226 4220651 -0.7 -1.19 -0.97 -1.07 -1.56 -1.63 -1.61 -1.23 -1.57 -0.81 -1.24 -1.10 -0.70 -1.61 -0.48<br />
6221 RB8098_hypothetical protein 4337334 4337119 -1.09 -1.63 -3.48 -2.79 -2.59 -0.31 -1.7 -1.1 -1.05 -1.5 -1.02 -0.08 -0.45 -0.48 0.07<br />
6426 RB8459_30S ribosomal protein S18 4518496 4518663 -1.39 -1.37 -1.35 -1.9 -1.48 -0.82 -0.72 -0.67 -0.45 -0.28 -1.50 -2.05 NaN -2.53 -1.11<br />
6546 RB8669_hypothetical protein 4614557 4614670 -1.08 -2.37 -4.19 -3.89 -2.87 -0.54 -2.04 -1.52 -1.2 -1.15 -2.00 -0.86 -1.92 -1.72 -0.61<br />
7007 RB9460_hypothetical protein 5078860 5078621 -1.24 -2.03 -4.04 -3.67 -2.89 -0.55 -1.41 -1.16 -0.9 -0.76 -2.47 -1.26 -1.99 -1.92 -1.67<br />
7282 RB9917_Single-strand binding protein/Primosomal replication protein n 5344370 5343861 -0.8 -1.36 -1.36 -1.2 -1.22 -0.65 -0.98 -0.61 -0.68 -0.28 -1.28 NaN NaN -2.32 -0.84
Cluster 2<br />
Original row UID START STOP heat_10min heat_20min heat_40min heat_60min heat_300min cold_10min cold_20min cold_40min cold_60min cold_300min salt_10min salt_20min salt_40min salt_60min salt_300min<br />
162 RB10261_Carbon storage regulator 5545964 5545719 0 -1.11 -1.88 -1.69 -1.81 1.22 0.99 0.78 1.23 0.15 -0.32 -0.05 0.95 -0.75 -0.62<br />
290 RB10483_conserved hypothetical protein 5685437 5685838 -1.38 -1.78 -1.34 -1.51 -1.42 NaN 0.01 0.2 0.24 0.64 -0.02 NaN NaN -0.05 0.96<br />
1922 RB13207_secreted protein 7086761 7086585 -0.59 -1.44 -1.24 -1.53 -1.15 0.5 1 1.18 1.45 -0.18 -0.14 -0.59 -0.11 0.26 -0.01<br />
2517 RB2247_conserved hypothetical protein containing YTV domains 1181238 1179499 -1.67 -1.77 -1.54 -1.37 -2.3 0.47 0.56 0.24 0.02 0.73 0.28 -0.49 NaN -0.53 0.37<br />
2518 RB2251_hypothetical protein 1181714 1181487 -1.84 -1.64 -1.33 -1.27 -2.64 0.31 0.73 0.33 0.14 0.47 0.20 -0.52 -0.26 -0.64 0.52<br />
4912 RB5957_secreted protein 3122093 3122557 -0.46 -1.32 -1.19 -1.21 -1.83 0.82 0.48 0.27 0.44 -0.51 -0.81 -0.79 -0.52 -0.89 -0.14<br />
5659 RB7178_hypothetical protein 3806924 3807061 -1.64 -1.96 -1.53 -1.78 -2.24 0.78 0.77 0.87 0.88 1.01 0.62 0.15 -0.72 -0.39 0.22<br />
5661 RB7180_hypothetical protein 3807345 3807031 -1.5 -1.84 -1.45 -1.58 -2.34 0.33 0.89 1.12 0.85 0.89 0.70 0.26 0.67 0.80 0.82<br />
5662 RB7184_conserved hypothetical protein 3807517 3808089 -1.58 -1.22 -0.63 -0.82 -1.92 0.25 1.1 0.86 0.77 0.45 0.02 -0.02 -0.30 -0.27 0.09<br />
5862 RB7525_secreted protein 4048930 4048085 NaN -1.49 -1.04 -1.35 -2.67 NaN 1 NaN NaN 0.8 0.35 NaN NaN -0.45 NaN<br />
5898 RB7578_Carbon storage regulator 4070466 4071035 -0.1 -1.77 -1.74 -1.94 -0.95 0.74 1.17 0.87 1.16 0.3 0.64 -0.32 -0.31 -0.27 -0.20<br />
6560 RB869_hypothetical protein 460848 461063 -0.99 -0.59 -0.29 -1.6 -2.13 -0.5 0.22 0.36 0.21 1.29 -0.49 0.04 NaN -0.28 0.38<br />
6771 RB9057_hypothetical protein 4834849 4834562 -1.08 -1.13 -1.3 -1.46 -1.7 0.02 0.49 0.7 0.5 0.01 0.88 0.72 1.47 0.93 0.64<br />
857 RB11429_conserved hypothetical protein 6196478 6196591 -1.4 -1.12 -0.76 -1.14 -2.38 -0.7 -0.21 0.11 -0.16 0.08 -0.13 0.12 0.66 0.00 0.54<br />
2615 RB2384_hypothetical protein 1242697 1242500 -1.27 -0.7 -0.65 -0.96 -1.73 0.27 -0.06 0.24 0.15 0.71 -0.75 -0.15 -0.39 -0.81 -0.37<br />
3228 RB3365_hypothetical protein 1730124 1730255 -1.8 -2.19 -3.89 -2.76 -3.2 -0.44 -0.12 -0.35 -0.4 -0.76 -0.28 0.38 0.96 0.18 1.03<br />
4081 RB4652_hypothetical protein 2380494 2380652 -1.2 -1.34 -1.3 -1.16 -1.61 -0.56 -0.33 -0.18 -0.52 -0.45 -0.05 -0.39 0.57 0.16 0.52<br />
4281 RB4941_hypothetical protein 2541619 2542140 -1.51 -0.83 NaN NaN -1.95 0.67 1.22 0.61 0.74 0.96 NaN NaN NaN NaN 0.40<br />
5414 RB6777_hypothetical protein 3602786 3603199 -0.74 -1.07 -1.31 -1.35 -1.59 1.43 1.32 1.06 0.99 -0.08 0.47 0.48 0.39 0.48 -0.44<br />
5863 RB7530_hypothetical protein 4049298 4049005 -2.97 -2.14 -1.8 -2.09 -3.24 NaN 0.47 -0.01 0.03 -0.01 0.12 NaN NaN NaN -0.27<br />
6366 RB8353_hypothetical protein 4459374 4459622 -2.57 -1.66 -1.64 -2.31 -3.27 NaN -0.04 0.12 0.04 0.02 0.49 -0.10 0.45 0.16 -0.51<br />
5118 RB6314_hypothetical protein 3319320 3319478 -2.29 -1.77 -1.72 -1.86 -3.31 -0.87 -0.68 -0.61 -0.6 -0.37 0.56 0.19 0.61 0.49 0.52<br />
5749 RB7313_hypothetical protein 3892097 3891942 -1.61 -1.9 -2.26 -1.91 -3.17 0.12 -0.02 -0.85 -0.3 -0.35 0.02 -0.53 -0.28 -1.00 0.31
Cluster 3<br />
Original row UID START STOP heat_10min heat_20min heat_40min heat_60min heat_300min cold_10min cold_20min cold_40min cold_60min cold_300min salt_10min salt_20min salt_40min salt_60min salt_300min<br />
2867 RB2784_membrane protein containing DUF107 1448065 1447454 0.98 1.99 0.95 2.25 1.93 0.25 0.93 0.73 0.32 0.74 0.35 0.08 -0.07 0.46 1.23<br />
3992 RB4517_Transcriptional coactivator/pterin dehydratase 2315930 2316283 1.1 1.27 1.85 1.61 1.86 0.36 0.68 0.83 0.36 0.91 0.21 NaN NaN 0.72 0.53<br />
4108 RB4698_hypothetical protein 2397601 2397861 0.62 1.35 1.57 2.15 2.83 NaN 0.51 0.24 -0.04 0.24 NaN NaN NaN NaN NaN<br />
4319 RB4995_6,7-dimethyl-8-ribityllumazine synthase 2569838 2569371 0.22 1.09 NaN NaN 2.19 0.51 1.14 0.79 0.65 1.22 NaN NaN NaN NaN NaN<br />
4436 RB5181_hypothetical protein 2668763 2668873 NaN 0.87 NaN 1.8 NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN<br />
5100 RB6282_conserved hypothetical protein 3302795 3303397 0.61 0.89 2.22 2.18 1.93 NaN 0.84 0.88 0.58 0.81 NaN NaN NaN -0.10 0.66<br />
5809 RB7437_LexA repressor 3987953 3988672 0.53 1.37 1.67 2.11 1.65 NaN 0.96 0.72 0.4 0.82 0.89 NaN NaN 0.14 1.73<br />
6144 RB7960_ATP:guanido phosphotransferase 4257723 4256632 1.5 1.47 1.8 1.25 1.93 0.04 0.69 0.6 0.42 1.03 NaN NaN NaN -0.04 1.12<br />
6400 RB8409_hypothetical protein 4495547 4495840 0.31 1.62 NaN NaN 1.5 NaN 0.99 1.19 0.76 1.54 -0.17 NaN NaN 1.22 1.06<br />
854 RB11421_60-kDa SS-A/Ro ribonucleoprotein 6195538 6193880 NaN 0.54 2.55 2.32 0.99 NaN NaN NaN NaN 0.27 NaN NaN NaN NaN NaN<br />
2062 RB1449_hypothetical protein 736500 736697 NaN 0.94 1.66 1.91 1.26 NaN 1.26 0.82 0.14 0.3 NaN NaN NaN 1.02 1.30<br />
2137 RB1573_hypothetical protein 805094 804984 0.47 1.41 0.29 2.22 2.19 NaN 1.24 NaN NaN 1.43 NaN NaN NaN NaN NaN<br />
2211 RB171_ISxac3 transposase 89626 90504 0.41 1.27 1.28 1.82 1.49 NaN 1.55 0.95 1.12 0.7 NaN NaN NaN 0.44 1.29<br />
2355 RB1977_mRNA 3'-end processing factor 1039488 1040645 1.14 1.85 1.55 2.05 1.56 0.78 1.09 0.76 0.49 0.86 0.24 NaN -0.17 0.68 0.65<br />
2848 RB2745_hypothetical protein 1431275 1431400 0.45 1.27 1.58 1.57 0.87 0.58 0.98 0.77 0.63 1.57 1.30 0.33 NaN 0.95 0.87<br />
2862 RB2774_conserved hypothetical protein 1443786 1444034 1.13 1.88 1.94 1.51 1.89 0.4 0.59 0.87 0.45 1.28 NaN NaN NaN -0.19 0.68<br />
2982 RB2963_hypothetical protein 1526707 1527066 0.78 2.31 1.96 1.79 1.89 0.76 0.5 0.61 0.54 1.24 0.37 0.12 NaN 0.63 -0.02<br />
3860 RB4322_transposase and inactivated derivative 2226866 2228239 1.1 1.49 1.36 1.58 2.02 NaN NaN NaN NaN 0.29 NaN NaN NaN 0.46 NaN<br />
4227 RB4867_SmpB RNA-binding protein 2496354 2495722 0.89 1.35 2.52 2.58 1.87 0.17 0.64 0.5 0.32 0.57 0.27 NaN NaN 1.06 0.23<br />
4365 RB5069_hypothetical protein 2609575 2609378 NaN 0.36 2.02 2.06 NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN<br />
4405 RB5135_6-pyruvoyl tetrahydrobiopterin synthase. 2640266 2639853 1.26 1.66 1.73 2.19 2.15 0.28 0.85 0.45 0.12 0.86 0.61 NaN NaN 0.61 NaN<br />
5355 RB6685_hypothetical protein 3554265 3554047 1.03 2 1.84 1.95 1.85 NaN 0.92 0.74 0.24 0.55 NaN NaN NaN 1.07 0.53<br />
5531 RB6973_tellurite resistance protein 3710584 3711420 1.11 1.56 1.79 2.07 2.67 NaN 0.76 0.53 0.17 0.64 NaN NaN NaN 0.17 NaN<br />
5571 RB7039_hypothetical protein 3743573 3742965 0.82 0.77 2.19 1.61 1.85 NaN NaN 0.55 NaN 0.15 NaN NaN NaN 2.01 1.18<br />
5923 RB7612_hypothetical protein 4092907 4093317 NaN 0.82 2.82 1.89 1.27 NaN 0.98 0.27 0.52 0.64 NaN NaN NaN NaN 1.16<br />
6420 RB8445_Penicillinase repressor 4514475 4514050 0.61 1.11 1.77 1.33 2.44 NaN 0.93 0.48 0.28 0.64 0.20 NaN NaN 1.26 1.95<br />
6474 RB8533_protease I 4552172 4552762 0.85 1.33 1.55 1.57 1.08 NaN 0.87 NaN 0.6 1.46 0.81 NaN NaN 1.06 1.63<br />
7308 RB9966_Fe-S oxidoreductase 5370040 5368655 NaN NaN NaN 1.58 NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN<br />
1068 RB11802_Transposase IS3/IS911 6361950 6362240 0.49 1.17 1.73 1.75 2.02 NaN 1.5 0.8 1 0.56 0.14 NaN NaN 0.93 1.10<br />
1476 RB12458_conserved hypothetical protein 6704719 6704976 NaN 0.79 2.67 1.95 0.92 NaN NaN NaN NaN 1.14 NaN NaN NaN NaN NaN<br />
2203 RB17_protein containing DUF1589 8251 7757 0.18 1.44 2.49 2.46 1.22 NaN NaN NaN 0.71 1.97 NaN NaN NaN NaN NaN<br />
2295 RB1862_transcriptional regulator, GntR family 960630 961037 NaN 0.75 1.85 1.33 NaN NaN NaN NaN NaN 1.46 NaN NaN NaN NaN NaN<br />
2457 RB2146_Exonuclease VII, small subunit 1136505 1136086 0.99 2.47 1.6 2.06 1.1 1.01 0.83 1.15 0.7 1.78 0.21 NaN 0.36 0.22 0.17<br />
2914 RB286_hypothetical protein 142779 143096 NaN 0.89 2.02 2.14 NaN NaN NaN NaN NaN 0.57 NaN NaN NaN NaN NaN<br />
3099 RB3159_protein containing DUF1590 1629337 1629116 1.62 1.17 1.53 1.47 1.45 0.16 0.72 0.99 0.8 1.16 1.09 NaN NaN 0.61 1.66<br />
5328 RB6637_hypothetical protein 3528317 3528469 0.59 1.72 1.63 2.34 0.9 NaN 1.68 1.37 0.92 1 NaN NaN NaN NaN NaN<br />
6349 RB8325_hypothetical protein 4447029 4446139 0.19 1.28 2.36 1.53 2.14 NaN 1.72 1.11 0.89 1.22 NaN NaN NaN NaN NaN<br />
6929 RB933_hypothetical protein 493451 492825 NaN 1.01 2.1 1.94 NaN NaN NaN NaN NaN 0.68 NaN NaN NaN NaN NaN<br />
7079 RB957_Tyrosine recombinase xerD 505476 504889 NaN 1.04 1.64 2.07 NaN NaN NaN NaN NaN 0.83 NaN NaN NaN NaN NaN<br />
7324 RB9998_ABC transporter ATP-binding protein 5385937 5385104 0.95 1.33 1.57 1.23 1.28 0.65 0.96 1.14 1.53 1.5 0.63 -0.25 NaN 0.47 0.89<br />
91 RB10137_protein containing DUF1590 5469772 5469551 0.86 1.66 2.03 2.31 2.03 NaN 1.34 0.79 0.58 0.87 NaN NaN NaN 0.03 2.12<br />
640 RB11077_Protein of unknown function, UPF0074 6006371 6005904 0.4 0.93 1.34 1.17 1.36 0.53 1.26 1 1.02 0.87 0.61 NaN NaN 0.55 1.99<br />
1162 RB11952_secreted protein 6438438 6438043 NaN 0.76 2.7 2.36 1.28 NaN NaN NaN NaN 0.61 NaN NaN NaN NaN NaN<br />
1689 RB12806_conserved hypothetical protein 6911165 6910548 NaN 0.99 1.27 2.06 2.3 NaN NaN NaN NaN 0.62 NaN NaN NaN NaN NaN<br />
1913 RB13190_conserved hypothetical protein 7083077 7083229 0.19 1.18 1.23 1.98 0.71 NaN 1.75 1.37 1.06 0.99 1.02 NaN NaN NaN NaN<br />
3673 RB402_hypothetical protein 212820 213275 0.47 1.13 2.33 2.63 2.14 1.29 1.23 1.23 0.47 1.35 NaN NaN NaN 0.19 0.89<br />
3862 RB4325_Planctomycete PGAMP 2228477 2228623 NaN 1.15 0.96 1.99 2.3 NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN<br />
4849 RB5848_dnaK suppressor protein, 3066328 3065906 1.75 1.67 1.84 1.79 2.39 0.65 0.81 0.98 0.84 1.04 0.45 -0.61 NaN 0.20 0.67<br />
5055 RB6207_conserved hypothetical protein, secreted 3252449 3253759 1.65 2.03 1.44 1.59 1.44 0.83 1.08 0.99 0.82 1.19 0.64 NaN -0.17 1.15 0.92<br />
5211 RB6449_myosin-like membrane protein 3400438 3406236 NaN 1.44 1.41 2.28 0.88 NaN NaN NaN NaN 2.15 NaN NaN NaN NaN NaN<br />
6385 RB8384_ISXo8 transposase 4478623 4479924 0.83 1.3 2.1 1.79 1.61 NaN NaN NaN 1.6 1.02 NaN NaN NaN NaN NaN<br />
6543 RB865_hypothetical protein 458731 460359 0.99 1.16 1.21 1.49 1.47 0.64 1.52 1.31 1.34 1.9 0.63 0.27 NaN 0.47 1.69<br />
7277 RB9908_hypothetical protein 5339497 5339649 0.41 1.33 1.8 2.42 1.23 NaN 1.79 1.61 1.1 1.17 NaN NaN NaN NaN NaN<br />
952 RB11603_conserved hypothetical protein 6256722 6256835 2.03 1.41 1.76 2.09 2.41 0.32 0.96 0.74 0.65 0.17 0.68 0.12 0.03 1.48 1.11<br />
1538 RB1256_hypothetical protein 641044 640889 NaN 1.77 1.64 2.66 1.57 NaN NaN NaN NaN 0.2 NaN NaN NaN NaN NaN<br />
1641 RB12732_secreted protein containing DUF1501 6862068 6860800 0.1 1.12 1.5 1.61 3.01 NaN 1.62 0.99 1.3 0.91 NaN NaN NaN NaN 1.82
3845 RB4300_integrase 2214826 2215704 1.46 1.04 1.36 1.23 1.13 -0.12 1 1.14 1.11 1.12 0.57 NaN NaN 1.16 1.56<br />
4638 RB550_secreted protein 306709 306296 1.65 2.18 2.32 2.16 2.04 -0.22 1.1 1 0.62 1.27 0.45 NaN 0.36 0.78 0.58<br />
4900 RB5934_Squalene--hopene cyclase 3112260 3110275 NaN 1.56 NaN NaN NaN NaN NaN NaN NaN 0.87 NaN NaN NaN NaN NaN<br />
5034 RB6167_Putative transposase 3235937 3237064 NaN 1.14 1.74 1.75 1.38 NaN 1.61 NaN 1.43 1.34 NaN NaN NaN 0.37 NaN<br />
5046 RB6188_hypothetical protein 3244384 3244178 1.11 1.09 1.73 1.36 1.54 NaN 1.26 NaN 1.22 1.34 0.76 NaN NaN 0.94 2.22<br />
5372 RB6709_Transposase IS3/IS911 3564146 3563856 1.4 1.26 1.39 1.33 2.08 NaN 1.13 0.91 1.14 0.88 0.39 NaN NaN 1.18 1.71<br />
6373 RB8363_peptidase 4463592 4462387 NaN 1.53 NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN<br />
1720 RB12851_conserved hypothetical protein 6926556 6926885 1.11 1.68 1.86 1.81 2.36 NaN NaN NaN NaN 1.25 NaN NaN NaN 0.42 NaN<br />
2881 RB2809_hypothetical protein 1459745 1459065 0.74 2.28 2.59 2.39 1.77 0.88 1.1 0.91 0.52 0.84 1.16 0.06 NaN 1.13 1.06<br />
4669 RB5551_hypothetical protein 2889101 2891092 1.79 0.83 2.31 1.82 2.59 NaN 1.5 0.61 0.7 0.66 NaN NaN NaN 0.34 2.16<br />
4949 RB6011_hypothetical protein 3150235 3150083 0.68 1.58 1.96 2.25 1.28 NaN 1.68 1.48 1.27 1.47 NaN NaN NaN NaN NaN<br />
5011 RB6131_hypothetical protein 3223482 3223790 0.69 1.22 1.44 1.16 1.18 0.19 0.53 1.31 0.92 1.65 0.85 0.31 NaN 1.64 1.57<br />
5020 RB6144_hypothetical protein 3228305 3228189 1.14 1.81 2.94 2.16 2.46 -0.08 0.74 0.74 0.33 1.21 0.75 NaN NaN 1.83 1.78<br />
5583 RB706_ISxac3 transposase 378430 379308 1.17 0.88 1.21 1.29 1.03 NaN 1.27 1.19 1.13 1.27 0.59 NaN NaN 0.90 1.64<br />
169 RB10271_ribonuclease H 5552547 5552071 1.02 1.24 1.97 1.77 1.41 0.58 1.58 1.09 1.38 1.91 NaN NaN NaN 0.93 1.06<br />
2481 RB2185_hypothetical protein 1152681 1152529 0.79 1.76 1.92 2.61 1.04 NaN NaN 1.84 1.37 1.45 NaN NaN NaN NaN NaN<br />
2532 RB2268_peptide methionine sulfoxide reductase 1191389 1190541 1.17 2.4 2.34 2.01 1.59 0.62 0.86 1.49 0.77 2.06 1.41 -0.02 0.08 0.58 0.59<br />
3376 RB3596_nitrogen fixation protein (NifU protein) 1868895 1869329 0.59 1.6 1.99 2.03 2.18 1.03 1.12 1.15 0.92 1.74 NaN 0.63 NaN 1.01 1.08<br />
4448 RB520_conserved hypothetical protein 284989 286116 1.73 2.32 1.47 2.04 2.31 0.25 1.09 0.69 0.51 1.02 0.52 NaN 0.80 1.29 1.02<br />
6756 RB9023_hypothetical protein 4808850 4809074 NaN NaN NaN 1.8 NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN<br />
863 RB11438_conserved hypothetical protein 6197926 6197762 NaN 0.7 2.55 2.86 2.12 NaN NaN NaN NaN 0.35 NaN NaN NaN NaN NaN<br />
1076 RB11813_conserved hypothetical protein 6366331 6366573 0.65 1.08 1.19 1.6 0.79 0.52 1.74 NaN 1.64 1.82 0.92 NaN NaN 0.92 1.70<br />
1101 RB11855_conserved hypothetical protein 6383765 6383460 NaN 1.06 1.57 1.62 1.59 NaN NaN NaN NaN 2.42 NaN NaN NaN 1.30 NaN<br />
1130 RB11909_conserved hypothetical protein 6410157 6410663 1.59 1.64 1.33 1.91 2.35 NaN 1.09 NaN NaN 0.91 NaN NaN NaN NaN NaN<br />
2300 RB1872_hypothetical protein 966053 966169 1.26 1.1 1.69 1.29 1.18 0.88 1.75 1.42 1.68 1.39 0.87 NaN NaN 0.70 1.96<br />
5035 RB6168_hypothetical protein 3236689 3237315 0.88 1.29 2.17 1.68 1.74 NaN 1.49 1.16 1.66 1.89 0.83 NaN NaN NaN NaN<br />
880 RB11475_conserved hypothetical protein 6209468 6209641 1.27 1.46 1.72 1.71 1.07 NaN NaN NaN 1.53 1.68 1.21 NaN NaN 1.43 2.30<br />
1427 RB12387_conserved hypothetical protein 6665894 6665742 0.58 1.63 1.91 2.63 1.15 NaN 2.01 1.87 1.48 1.27 NaN NaN NaN NaN NaN<br />
1719 RB12850_Rhodopirellula transposase 6926553 6925624 1.47 1.81 2.07 1.71 2.16 NaN 1.38 0.81 1.02 1.15 NaN NaN NaN 0.99 NaN<br />
2031 RB1394_hypothetical protein 711994 711758 1.68 1.17 1.14 0.83 2.54 0.79 1.03 1.64 1.8 1.08 1.05 0.01 0.05 1.10 1.21<br />
5390 RB6736_ISXo8 transposase 3578852 3577551 NaN 1.44 2.13 1.82 1.44 NaN NaN 1.39 1.4 1.04 NaN NaN NaN NaN NaN<br />
5400 RB6751_chaperone ClpB 3586111 3588690 1.94 1.74 1.51 1.57 2.12 0.87 1.27 0.77 1.09 1.45 NaN NaN NaN 0.24 1.21<br />
5697 RB723_hypothetical protein 388051 388233 1.16 1.16 1.42 1.29 1.53 0.44 2.21 1.61 1.82 0.75 1.03 NaN NaN 0.99 2.37<br />
7066 RB955_hypothetical protein 504140 503514 NaN 1.25 2.49 2.5 1.37 NaN 1.66 NaN NaN 1.03 NaN NaN NaN NaN NaN<br />
180 RB10292_conserved hypothetical protein, secreted 5559646 5561391 2.36 1.38 1.43 1.25 2.65 0.29 1.38 0.77 0.77 0.33 0.68 NaN NaN 0.91 2.01<br />
4679 RB5567_hypothetical protein 2897766 2897566 1.82 1.95 2.84 2.55 2.64 0.45 0.79 0.49 0.46 0.5 NaN NaN NaN 1.07 1.55<br />
4950 RB6012_ISXo8 transposase 3151547 3150246 NaN 1.3 2.31 2 NaN NaN NaN NaN NaN 0.81 NaN NaN NaN NaN NaN<br />
2256 RB1789_conserved hypothetical protein 925861 926592 1.6 1.27 1.52 1.44 1.85 0.85 1.61 1.41 1.57 1.66 0.63 NaN NaN 0.39 1.10<br />
3611 RB3929_hypothetical protein 2034407 2034243 NaN 1.17 2.41 2.79 1.83 NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN<br />
6933 RB934_Putative transposase 494203 493076 0.99 1.44 2.21 2.24 1.63 0.39 1.81 NaN 1.86 1.84 NaN NaN NaN 1.28 NaN<br />
1277 RB12150_conserved hypothetical protein 6556901 6557062 2.39 0.99 1.63 1.08 2.5 0.27 0.87 1.22 0.92 0.84 1.04 0.27 0.50 1.83 1.61<br />
4552 RB5371_hypothetical protein 2774829 2775158 1.52 1.51 1.62 1.76 2.23 NaN 1.85 NaN NaN 1.04 NaN NaN NaN NaN NaN<br />
5369 RB6706_hypothetical protein 3560942 3560829 1.61 1.64 1.94 1.78 1.96 NaN NaN 1.27 1.25 1.13 NaN NaN NaN NaN 1.31<br />
1119 RB1189_Transposase IS3/IS911 606838 607128 1.78 1.36 1.58 1.62 2.24 NaN 1.36 1.16 1.32 1.04 NaN NaN NaN 1.27 1.81<br />
1135 RB11918_protein containing DUF971 6415701 6415297 1.94 1.36 1.56 1.31 1.58 0.55 1.87 1.29 1.42 2.14 0.61 NaN NaN 0.92 1.73<br />
1525 RB1254_hypothetical protein 640610 640747 1.42 0.87 1.27 1.36 1.05 NaN 1.38 1.1 0.9 0.62 0.77 NaN NaN 1.07 2.44<br />
4656 RB5523_hypothetical protein 2860984 2860709 1.79 2.07 2.19 2 1.98 0.06 1.31 1.11 1.02 2.52 NaN NaN NaN 0.31 0.80<br />
5534 RB6982_ABC transporter ATP-binding protein 3715019 3715879 1.73 2.07 1.16 2.5 2.8 NaN 1.2 0.84 0.74 1.18 0.10 NaN NaN 0.88 NaN<br />
6264 RB8177_membrane protein 4371052 4375008 1.19 1.53 NaN 0.56 1.96 0.19 2.31 1.68 1.68 0.23 NaN NaN NaN NaN 0.99<br />
6374 RB8365_membrane protein 4463903 4463631 1.68 2.07 2.01 1.91 1.58 1.34 1.44 1.32 1.35 1.78 0.35 -0.39 NaN 0.64 0.39<br />
7303 RB9955_secreted protein containing DUF1552 5363605 5362247 2.2 1.61 1.23 1.54 2.42 NaN 1.17 0.94 1.44 2.27 -0.05 NaN NaN 0.65 1.48<br />
7319 RB9986_hypothetical protein 5380717 5380379 NaN 1.13 2.34 NaN 1.46 0.48 1.66 1.46 1.08 1.75 NaN NaN NaN 0.26 0.35<br />
1046 RB11750_integrase 6328357 6329235 1.85 1.37 1.62 1.64 1.31 NaN 1.69 1.68 1.46 1.44 0.91 NaN NaN 1.59 2.02<br />
1178 RB11981_ABC transporter (substrate-binding protein) 6459460 6460413 0.65 0.9 0.98 1.25 0.92 1.22 1.57 1.13 1.45 1.58 0.89 0.13 NaN 0.80 1.41<br />
1852 RB13093_secreted protein 7025577 7025101 0.75 0.71 2.81 1.96 2.38 1.01 1.86 1.59 1.7 1.59 NaN NaN NaN 0.45 -0.42<br />
4551 RB5370_Rhodopirellula transposase 2774826 2773897 2.09 1.52 1.6 1.43 2.23 NaN 1.73 1.19 1.3 1.34 NaN NaN NaN 0.64 2.11<br />
4859 RB5867_hypothetical protein 3077578 3077730 NaN 1.38 1.24 1.92 0.85 NaN 1.9 1.42 1.13 0.88 NaN NaN NaN NaN NaN<br />
5176 RB6395_conserved hypothetical protein 3364879 3367215 1.71 2.06 1.92 2.57 2.44 0.7 1.39 0.97 0.79 1 0.62 NaN NaN 0.74 0.98<br />
6851 RB9198_hypothetical protein 4918032 4918208 NaN NaN 0.99 2.24 NaN NaN NaN NaN NaN 1.44 NaN NaN NaN NaN NaN
1124 RB1190_integrase 607131 608009 1.49 0.98 0.98 1.36 1.31 0.09 1.29 1.32 1.33 0.84 0.76 NaN NaN 1.51 1.74<br />
5323 RB663_hypothetical protein 357608 357369 1.71 2.53 1.69 2.53 2.32 0.58 0.86 0.71 0.7 0.84 1.24 0.49 0.32 1.78 0.95<br />
1175 RB11977_conserved hypothetical protein 6455958 6456548 1.94 1.5 1.06 1.13 2.45 NaN 1.17 1.41 1.63 1.39 1.79 0.35 0.17 1.49 0.39<br />
1690 RB12808_secreted protein 6912350 6911241 NaN 1.48 2.14 2.29 2.46 NaN 1.57 0.83 NaN 0.71 NaN NaN NaN NaN 1.58<br />
697 RB11176_protein containing DUF442 6065605 6065144 1.86 1.3 1.31 1.03 1.68 1.47 2.33 1.86 2.06 1.26 0.54 -0.05 0.84 0.70 1.63<br />
960 RB11618_type I restriction-modification system, M subunit, 6263708 6265366 NaN 1.36 2.25 2.3 2.42 NaN NaN NaN NaN 0.62 NaN NaN NaN NaN NaN<br />
5572 RB704_Transposase IS3/IS911 378137 378427 1.75 1.52 1.89 1.73 2.26 1.47 1.49 1.14 1.36 0.87 0.78 NaN NaN 1.33 1.83<br />
574 RB10966_Protein of unknown function, UPF0057 5942934 5942752 1.13 1.54 1.07 1.44 1.22 1.43 1.67 1.45 1.62 0.97 0.80 0.53 0.45 0.99 0.15<br />
5778 RB7389_Putative transposase 3953590 3954717 1.02 1.7 2.58 2.39 1.82 0.73 1.95 NaN 1.87 2.4 1.33 NaN NaN 1.18 NaN<br />
6940 RB9347_hypothetical protein 5008600 5008394 1 0.64 1.08 0.95 1.24 0.64 1.24 1.52 1.39 1.67 1.23 NaN NaN 0.71 1.87<br />
6469 RB8527_protein containing DUF1559 4549097 4550089 1.09 0.6 1.26 1.4 1.54 2.15 2.54 2.05 2.08 0.75 0.88 0.11 NaN 0.50 1.90<br />
1685 RB12800_conserved hypothetical protein 6908240 6908710 0.56 1.08 1.16 1.47 1.3 0.89 1.61 NaN NaN 1.92 NaN NaN NaN NaN NaN<br />
6454 RB8505_serine/threonine protein kinase related protein 4538446 4537127 0.89 0.8 1.53 1.13 0.82 1.12 1.57 1.56 1.56 1.12 1.39 0.11 0.55 0.14 0.52
Cluster 4<br />
Original row UID START STOP heat_10min heat_20min heat_40min heat_60min heat_300min cold_10min cold_20min cold_40min cold_60min cold_300min salt_10min salt_20min salt_40min salt_60min salt_300min<br />
168 RB10270_phytoene dehydrogenase 5551969 5550356 2.81 2.15 1.46 1.89 2.82 NaN NaN NaN NaN 0.6 0.22 NaN NaN 1.03 NaN<br />
2204 RB170_Transposase IS3/IS911 89333 89623 2.16 2.34 2.99 2.58 2.59 NaN 1.99 1.46 1.54 1.47 NaN NaN NaN 1.65 1.34<br />
3935 RB4438_Pyridoxamine 5'-phosphate oxidase- 2278247 2278729 1.77 2.65 2.52 2.37 2.88 1.37 1.7 1.79 1.44 1.94 1.43 0.68 1.95 1.69 0.87<br />
37 RB10049_RNA polymerase ECF-type sigma factor 5419507 5420160 3.14 2.35 1.46 1.8 3.18 1.19 1.76 1.78 1.82 1.17 1.06 NaN NaN 1.80 1.27<br />
174 RB10279_heat shock protein, Hsp20 family 5557118 5557573 2.84 2.75 3.26 3.43 4.02 NaN 0.82 0.15 0.29 -0.16 0.23 0.26 NaN 0.25 -0.19<br />
175 RB10283_small heat shock protein 5557737 5558060 4.58 3.65 3.68 3.44 4.53 1.12 2.11 1.13 1.36 0.77 1.11 0.72 NaN -0.02 0.12<br />
227 RB10378_Thioredoxin 5610497 5610063 1.15 1.08 1.3 0.95 1.7 1.96 1.86 1.93 2.17 1.51 1.62 1.18 1.49 1.21 0.32<br />
393 RB10665_hypothetical protein 5789912 5790514 1.75 1.7 1.95 2.35 1.67 1 1.88 1.27 1.19 0.91 2.08 1.61 1.48 2.55 1.40<br />
431 RB10728_secreted protein 5831649 5832047 2.96 1.76 1.22 1.68 3.22 2.48 2.76 2.28 2.67 1.29 2.07 0.36 NaN NaN 0.83<br />
534 RB10896_secreted protein 5917040 5916555 2.1 0.74 1.24 1.32 2.06 2.08 2.24 2.03 2.9 1.48 1.74 0.38 0.42 1.33 0.63<br />
557 RB10936_hypothetical protein 5934348 5934518 2.64 1.63 1.78 1.12 2.91 NaN NaN NaN NaN 1.52 NaN NaN NaN NaN NaN<br />
568 RB10954_hypothetical protein 5940749 5940318 1.78 1.56 1.7 1.06 1.33 0.54 1.91 1.92 2.08 1.66 0.31 1.15 3.01 1.15 -0.36<br />
751 RB11260_dnaK suppressor protein, 6107202 6106837 2.55 1.66 1.53 1.15 1.85 0.91 2.02 1.46 2.02 1.82 0.85 0.12 1.11 1.08 0.45<br />
838 RB11397_SOUL heme-binding protein 6178540 6179163 3.24 1.45 0.66 1.73 2.66 1.78 1.87 1.98 2.16 1.51 0.46 -0.19 0.00 0.87 0.68<br />
839 RB11399_Putative thiol-disulphide oxidoreductase DCC 6179167 6179565 3.28 2.09 0.91 1.03 2.58 1.65 1.86 1.89 2.22 1.84 0.55 -0.44 0.05 0.42 0.56<br />
901 RB11504_conserved hypothetical protein 6221285 6221067 3.88 2.65 1.32 1.84 3.56 1.47 2.2 2.56 2.1 1.47 1.97 1.10 3.37 3.38 1.17<br />
907 RB11515_conserved hypothetical protein 6224420 6224644 NaN 3.69 4.04 4.65 3.72 0.39 NaN NaN NaN 2.74 NaN NaN NaN 1.79 NaN<br />
935 RB11566_conserved hypothetical protein 6243111 6243698 2.45 1.91 2.2 1.55 2.51 1.16 NaN 1.77 1.85 1.84 0.59 NaN NaN -0.03 1.23<br />
1044 RB11749_Transposase IS3/IS911 6328064 6328354 2.08 2.18 2.92 2.56 2.61 0.34 1.75 1.54 1.38 1.07 0.80 NaN NaN 1.53 1.64<br />
1136 RB11919_Dihydroxy-acid and 6-phosphogluconate dehydratase 6417713 6415698 2.1 2.4 1.34 2.54 2.51 NaN 1.69 NaN NaN 1.21 NaN NaN NaN NaN NaN<br />
1230 RB12067_conserved hypothetical protein 6510236 6510559 2.71 1.93 2.49 1.78 2.08 NaN 2.25 1.57 1.82 2.73 0.06 NaN 0.87 0.16 0.46<br />
1336 RB12243_conserved hypothetical protein 6596256 6596423 NaN 2.82 1.74 2.35 3.19 NaN NaN 0.91 NaN 0.71 1.11 NaN NaN 3.56 NaN<br />
1338 RB12247_conserved hypothetical protein 6597048 6596824 4.03 1.35 0.91 1.62 3.47 1.76 1.71 1.75 2.07 0.76 NaN 0.26 NaN 2.33 0.53<br />
1353 RB12276_conserved hypothetical protein 6605112 6605444 3.04 1.6 1.31 1.29 3.7 NaN 1.39 NaN NaN 0.28 1.41 1.11 2.02 2.62 2.88<br />
1530 RB1255_hypothetical protein 640910 640755 NaN 1.56 1.96 3.22 2.13 NaN NaN NaN NaN 0.67 NaN NaN NaN NaN NaN<br />
1650 RB12746_Formamidopyrimidine-DNA glycolase 6869843 6870727 3.07 2.05 1.91 2.07 3.87 NaN NaN NaN NaN 0.87 0.18 NaN NaN 1.38 0.11<br />
1707 RB12830_conserved hypothetical protein 6920508 6920200 2.35 2.07 2.16 1.83 2.5 0.42 1.41 1.37 1.36 2.11 0.60 NaN NaN 0.46 0.48<br />
1708 RB12831_Glycosyltransferase 6921326 6920532 2.57 2.11 2.1 1.53 2.55 0.88 1.46 1.31 1.46 2.12 0.72 NaN NaN -0.06 -0.16<br />
1709 RB12833_conserved hypothetical protein 6921305 6921634 2.37 1.37 1.28 0.99 1.64 0.51 1.43 1.38 1.45 1.09 1.69 0.67 1.25 1.14 0.86<br />
1766 RB12936_conserved hypothetical protein 6965872 6964130 2.98 2.24 1.75 2.1 2.84 1.58 2.17 1.67 2.01 1.32 0.69 NaN NaN 1.32 0.93<br />
1768 RB12940_Transposase IS3/IS911 6966246 6966536 1.92 1.99 2.44 2.31 2.5 0.17 1.76 1.36 1.57 1.4 NaN NaN NaN 1.53 1.81<br />
1885 RB13142_Planctomycete PGAMP 7051226 7050849 NaN 1.81 3.01 3.02 2.95 NaN NaN NaN NaN 0.53 NaN NaN NaN NaN NaN<br />
1933 RB13222_SOUL heme-binding protein 7094714 7094088 NaN 4.96 3.54 5.02 NaN 0.57 2.95 NaN NaN 3.86 1.32 NaN NaN 1.72 0.55<br />
1954 RB13257_2-hydroxy-6-ketonona-2,4-dienedioic acid hydrolase 7105590 7106450 2.31 1.99 2.15 2.04 2.67 1.46 2.28 1.99 2.47 1.53 2.28 1.09 0.77 1.91 1.03<br />
2023 RB138_Sigma factor, ECF-like 60872 61516 2.67 2.15 2.18 1.98 3.59 0.3 1.81 1.17 1.3 1.28 0.51 NaN NaN 1.38 1.93<br />
2219 RB1722_hypothetical protein 897688 899643 NaN 2.57 1.26 2.08 4.18 NaN NaN NaN NaN -0.12 NaN NaN NaN 1.81 NaN<br />
2221 RB1728_membrane protein containing DUF1206 899954 900811 2.71 1.67 1.42 1.27 2.42 NaN NaN 1.07 NaN 1.01 0.86 NaN 0.73 1.74 0.86<br />
2222 RB1729_hypothetical protein 901146 900784 2.76 2.09 1.4 1.83 2.95 NaN NaN NaN NaN 0.82 1.13 NaN NaN 3.11 1.19<br />
3118 RB3196_hypothetical protein 1650093 1650422 1.35 2.98 2.87 2.62 2.2 0.72 0.88 1.31 0.59 1 1.47 1.22 1.49 2.28 1.98<br />
3573 RB3871_transposase of IS5377-like element 2001638 2002954 NaN 2.86 NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN<br />
3842 RB4299_Transposase IS3/IS911 2214533 2214823 2.37 1.83 2.19 2.1 2.72 0.67 1.89 1.66 1.46 1.63 1.03 NaN NaN 1.81 1.69<br />
3907 RB4397_protein containing DUF1560 2263976 2264143 2.96 1.84 1.69 1.93 2.8 1.03 2.53 1.89 1.93 0.73 1.77 0.36 1.41 2.56 1.92<br />
3927 RB4426_hypothetical protein 2275786 2275604 1.87 1.47 2.12 1.81 2.65 NaN NaN NaN NaN 0.75 NaN NaN NaN 1.31 NaN<br />
3933 RB4433_Ferritin and Dps 2277208 2277696 2.04 2.09 2.15 2.15 2.39 1.68 2.19 2.25 1.95 3.36 0.73 0.32 1.48 1.20 0.14<br />
4193 RB4811_ISPg4, transposase 2465768 2467084 NaN 3.18 0.82 2.21 3.4 NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN<br />
4194 RB4812_secreted protein 2467011 2467289 NaN 2.51 1.26 1.89 2.73 NaN NaN NaN NaN 0.77 NaN NaN NaN NaN NaN<br />
4441 RB519_hypothetical protein 285036 284872 NaN 3.4 1.73 3.64 NaN NaN NaN NaN NaN 0.77 NaN NaN NaN NaN NaN<br />
4454 RB521_hypothetical protein 286399 286208 2.31 3.53 1.7 3.68 3.4 0.58 1.78 1.61 0.88 0.9 2.10 0.86 2.64 3.43 2.06<br />
4473 RB5238_hypothetical protein 2697775 2697996 2.22 2.97 2.66 2.71 3.64 1.18 1.77 1.77 1.55 2.57 0.91 NaN NaN 2.06 -0.36<br />
4876 RB5888_Transposase IS3/IS911 3087636 3087346 2.09 1.93 2.22 2.23 2.46 0.74 1.83 1.7 1.49 1.31 1.10 NaN NaN 1.59 2.02<br />
4902 RB5938_hypothetical protein 3113484 3112372 2.78 3.47 2.57 3.21 4.02 1.95 2.41 2.44 2.01 3.07 0.90 NaN 0.11 1.77 1.28<br />
5223 RB6469_ABC transporter ATP-binding protein 3419515 3420273 2.01 2.43 2.3 2.19 2.6 0.35 1.11 0.9 0.67 1.5 NaN NaN NaN 1.33 0.64<br />
5483 RB6888_secreted protein containing DUF1573 3662449 3661187 2.43 2.14 2.92 2.56 3.35 NaN 1.01 0.61 0.36 0.4 NaN NaN NaN 1.23 0.86<br />
5506 RB6928_hypothetical protein 3678479 3678961 1.51 1.3 0.93 0.75 1.8 2.84 3.25 2.53 2.76 1.05 1.12 NaN NaN 0.85 2.64<br />
5772 RB7381_membrane protein 3950326 3949595 2.37 2.34 2.98 2.54 3.08 NaN 1.47 1.04 0.6 0.88 0.52 0.08 NaN 0.90 1.00<br />
5926 RB7616_protein containing DUF162 4094752 4094087 2.27 1.96 0.91 2.19 2.44 0.69 1.96 1.08 1.45 1.08 NaN NaN NaN NaN 1.08
6247 RB8146_hypothetical protein 4355122 4354667 2.38 1.9 1.8 1.46 2.31 0.5 0.95 1.18 1.43 1.21 0.86 0.07 0.98 1.71 1.45<br />
6475 RB8534_hypothetical protein 4552802 4552930 NaN NaN 1.79 2.24 NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN<br />
6726 RB8969_Chaperonin Cpn10 4779554 4779859 3.13 1.42 1.02 2.03 2.8 NaN NaN NaN NaN 0 NaN NaN NaN NaN NaN<br />
6728 RB8970_GroEL 4779905 4781527 2.95 2.09 1.45 1.92 2.31 0.9 1.7 0.94 1.52 1.6 NaN NaN NaN 0.44 0.59<br />
6732 RB8976_conserved hypothetical protein 4783568 4783846 NaN 2.49 NaN 3.55 NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN<br />
6739 RB8987_hypothetical protein 4790856 4790710 NaN 2.38 2.4 3 2.86 NaN 1.93 NaN 1.51 1.27 NaN NaN NaN 1.13 NaN<br />
6798 RB9105_Heat shock protein Hsp70 4868834 4866720 3.47 2.63 1.82 2.12 3.28 0.27 1.39 0.44 0.99 0.62 0.54 NaN NaN 0.82 0.19<br />
6870 RB9230_hypothetical protein 4936673 4936996 2.43 1.64 2.13 2.07 2.1 0.78 2.2 1.84 1.43 1.64 NaN NaN NaN 0.59 1.73<br />
6879 RB924_hypothetical protein 490400 489774 NaN 1.89 3.08 2.52 NaN NaN NaN NaN NaN 1.11 NaN NaN NaN NaN NaN<br />
7203 RB9787_hypothetical protein 5270904 5270719 NaN 2.32 3.29 3.54 2.73 NaN NaN NaN NaN 0.99 NaN NaN NaN NaN NaN<br />
7221 RB9810_transposase of IS5377-like element 5283122 5284438 NaN 2.83 NaN 1.64 NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN<br />
7222 RB9811_secreted protein 5284365 5284673 1.92 2.37 1.61 1.76 2.44 NaN 1.42 1.01 0.75 0.97 0.68 NaN NaN 2.79 1.61<br />
7276 RB9907_ISXo8 transposase 5338185 5339486 1.44 1.83 1.92 1.7 1.83 NaN 1.74 1.79 1.46 1.23 1.58 NaN NaN 1.95 NaN<br />
7325 RB9999_conserved hypothetical protein 5386828 5385983 NaN 1.72 2.72 2.41 2.49 0.6 2.41 1.95 2.81 2.1 0.35 NaN NaN 1.25 1.35<br />
519 RB10877_membrane protein 5905128 5903767 2.42 2.15 1.6 2.35 2.78 NaN 1.1 0.58 0.34 1.55 NaN NaN NaN 0.26 0.08
Cluster 5<br />
Original row UID START STOP heat_10min heat_20min heat_40min heat_60min heat_300min cold_10min cold_20min cold_40min cold_60min cold_300min salt_10min salt_20min salt_40min salt_60min salt_300min<br />
555 RB10932_secreted protein 5933872 5934069 0.76 0.47 0.14 -0.12 0.48 1.26 1.73 1.81 1.89 2.15 -0.81 -1.20 -0.43 -1.56 0.08<br />
556 RB10934_CsbD-like 5934135 5934341 1 0.72 0.12 0.4 0.2 1.55 1.92 1.57 1.54 1.5 -1.22 -1.26 -0.62 -1.47 0.09<br />
564 RB10948_conserved hypothetical protein, secreted 5938575 5939036 1.94 1.69 0.32 0.74 1.55 1.28 1.34 0.93 1.13 0.95 -1.98 -1.74 -1.15 -1.89 -0.87<br />
1935 RB13225_ATP-dependent DNA helicase RecQ 7096300 7097982 1.89 1.24 0.64 0.04 0.9 NaN 1.06 0.58 1.03 1.14 -0.34 NaN NaN -0.67 -0.82<br />
5179 RB6403_hypothetical protein 3369939 3370853 1.39 0.79 0.67 0.46 0.76 1.05 1.34 1.57 1.92 2.01 -0.32 NaN 0.22 -1.47 -1.84<br />
5417 RB6780_RNA polymerase sigma factor rpoD 3603381 3605054 0.8 0.43 0.47 0.75 0.25 1.13 1.77 1.55 1.42 1.06 -0.18 NaN -0.18 -0.19 -0.40<br />
6273 RB8194_secreted protein containing DUF1559 4382684 4383916 1.47 0.64 0.82 0.52 0.52 0.29 0.94 1.02 1.24 0.84 -0.27 -0.61 0.07 -0.67 -0.94<br />
554 RB10931_hypothetical protein 5933742 5933906 1.03 0.6 0.41 0.25 0.77 1.56 2.31 2.32 2.17 1.92 -0.60 NaN -0.67 -1.35 -0.15<br />
559 RB10939_conserved hypothetical protein 5934628 5934816 1.22 0.71 0.79 0.65 0.71 1.71 1.82 1.91 1.96 1.91 0.17 -0.54 -0.35 -0.58 -0.01<br />
570 RB10957_conserved hypothetical protein 5940708 5941007 1.68 0.77 -0.35 -0.13 0.55 1.95 2.34 2.58 2.38 2.05 -0.28 -0.28 1.06 -0.47 -1.14<br />
578 RB10971_Short-chain dehydrogenase/reductase SDR 5946406 5945495 2.05 0.93 0.63 0.23 1.59 0.82 1.79 1.33 1.87 1.08 0.17 -0.49 0.28 0.26 -0.55<br />
1229 RB12066_conserved hypothetical protein 6510147 6510554 1.54 0.39 0.51 0.28 0.55 1.13 1.57 1.49 1.79 0.5 0.40 -0.52 1.22 0.20 -0.18<br />
2027 RB1389_hypothetical protein 708892 708434 1.42 0.54 -0.14 0.28 1.69 NaN 0.88 0.74 1.57 1.44 -0.30 NaN NaN 0.63 0.02<br />
3414 RB3647_hypothetical protein 1895447 1895295 NaN 0.45 NaN NaN 0.73 NaN NaN 1.25 NaN 1.41 NaN NaN NaN NaN NaN<br />
3811 RB4256_conserved hypothetical protein, secreted 2180303 2181379 1.65 1.26 0.43 0.64 1.32 0.82 1.17 0.93 1.08 1.89 -0.60 -0.72 0.58 -0.68 -0.94<br />
3897 RB4383_decarboxylase 2258768 2257131 1.4 1.26 0.83 0.86 1.57 1.25 1.42 1.61 1.07 2.06 -0.32 NaN NaN -0.71 -0.48<br />
3961 RB4474_CsbD-like 2295048 2295554 1.09 0.96 0.71 0.5 0.5 1.6 1.37 1.28 1.44 1.96 -0.14 0.86 1.59 0.42 -0.64<br />
3962 RB4476_hypothetical protein 2295557 2295943 1.32 1.32 0.54 0.75 0.61 1.11 1.24 0.87 1.3 1.68 -0.05 0.64 1.57 0.35 -0.72<br />
4475 RB5240_secreted protein 2698074 2698631 0.72 0.92 1.36 0.98 0.77 0.71 0.88 1.05 0.93 1.43 0.42 -0.04 -0.14 0.25 -0.74<br />
4555 RB5377_membrane protein 2776583 2776104 0.66 0.69 1.34 1.17 0.55 1.03 1.49 1.1 0.95 1.08 0.63 0.11 NaN 0.26 -0.81<br />
4629 RB548_1,4-alpha-glucan branching enzyme 306230 304329 1.39 0.7 0.49 0.7 0.87 1.01 1.72 1.2 1.1 1.5 -0.03 -0.01 0.18 NaN -0.77<br />
5161 RB6379_hypothetical protein 3353122 3352661 1.99 1.61 1.5 1.38 1.37 1.59 2.44 2.15 2.2 1.68 -0.44 -1.08 NaN -1.37 -1.10<br />
50 RB10077_secreted protein 5436817 5437083 0.97 0.59 0.69 0.52 0.51 1.66 2.04 2.02 2.06 2.17 0.45 NaN 0.44 -0.35 0.23<br />
558 RB10937_hypothetical protein 5934561 5934376 2.34 0.9 0.92 0.66 1.51 NaN 1.68 1.27 1.38 1.45 -0.28 -0.60 0.60 0.34 0.33<br />
566 RB10950_membrane or secreted protein 5939124 5939825 2.64 0.96 1.05 0.77 1.55 NaN 1.37 0.89 1.1 1.39 -0.42 NaN NaN NaN -0.58<br />
569 RB10956_hypothetical protein 5940336 5940689 2.14 1.39 1.35 0.95 1.24 2 2.9 2.72 2.94 3.24 -0.01 -0.05 1.36 -0.29 -1.05<br />
571 RB10958_hypothetical protein 5941015 5941491 1.6 0.9 0.47 0.57 0.77 1.81 1.91 1.74 2.13 1.28 0.33 0.76 1.41 0.44 -0.73<br />
575 RB10967_quinone oxidoreductase-like protein 5944040 5943015 1.71 0.43 0.29 0.14 1.18 0.96 1.41 1.35 1.38 0.99 0.22 0.60 1.53 0.79 -0.08<br />
681 RB11150_NADH:flavin oxidoreductase/NADH oxidase 6046862 6048019 1.51 1.03 0.31 0.56 1.32 1.46 1.28 1.12 1.36 0.72 1.45 0.55 0.18 0.40 -0.69<br />
1114 RB11878_peptide methionine sulfoxide reductase msrA 6396598 6397104 2.32 1.06 0.93 0.2 2.06 1.12 1.66 1.24 1.61 0.69 -0.14 -0.31 NaN 0.24 0.14<br />
2029 RB1391_cell division inhibitor- 710780 709164 1.84 0.65 0.16 0.14 2.02 0.88 1.45 1.66 2.53 1.06 0.30 -0.22 0.56 0.77 0.25<br />
2555 RB2303_RNA POLYMERASE SIGMA-E FACTOR 1204509 1205219 0.99 1.22 NaN 1.42 1.21 0.99 1.26 0.72 0.84 2.04 NaN NaN NaN -0.11 0.15<br />
3872 RB4347_conserved hypothetical protein 2235735 2236205 1.57 1.49 0.55 0.55 1.14 1.71 1.69 1.93 1.74 2.61 -0.15 0.65 1.44 1.22 -1.06<br />
3930 RB4429_conserved hypothetical protein 2275996 2276265 1.62 1.57 1.33 0.93 1.77 0.94 1.19 1.4 1.21 2.29 0.07 -0.51 1.16 -0.57 -0.81<br />
5275 RB655_conserved hypothetical protein, UCP012641 family 354993 353953 1.74 1.62 0.13 1.2 1.51 0.57 1.57 0.81 0.94 2.32 NaN NaN NaN -0.22 NaN<br />
7321 RB9990_hypothetical protein 5381822 5381983 NaN NaN NaN 0.91 NaN NaN NaN NaN NaN 2.14 NaN NaN NaN NaN NaN<br />
192 RB10319_hypothetical protein 5577574 5577771 1.63 1.38 0.52 0.78 1.46 0.81 1.83 2.23 1.98 1.41 NaN NaN NaN NaN NaN<br />
1936 RB13228_conserved hypothetical protein 7097952 7098302 2.64 1.45 1.11 1.31 1.14 NaN NaN NaN NaN 1.85 NaN NaN NaN 0.18 0.17<br />
2807 RB2682_hypothetical protein 1393781 1393530 1.11 0.9 1.08 0.58 1.3 1.39 1.59 1.56 1.23 1.77 1.03 0.13 1.55 0.75 -0.14<br />
656 RB11107_secreted protein 6021425 6022102 1.37 0.92 0.75 0.94 1.44 0.98 NaN 2.16 2.42 2.37 NaN NaN NaN NaN 1.43<br />
1855 RB13097_membrane protein 7027310 7026795 NaN NaN 1.62 0.86 1.62 0.85 1.37 1.48 1.56 1.45 NaN NaN NaN NaN NaN<br />
5819 RB7451_hypothetical protein 3994530 3994706 NaN NaN NaN NaN NaN NaN NaN NaN NaN 1.87 NaN NaN NaN NaN NaN<br />
237 RB10396_conserved hypothetical protein, membrane 5620857 5620009 NaN NaN NaN NaN NaN NaN NaN NaN NaN 1.62 NaN NaN NaN NaN NaN<br />
6028 RB7765_secreted protein 4160757 4160044 NaN NaN 0.79 0.63 NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN<br />
6980 RB9411_ISPg4, transposase 5048517 5049893 NaN 1.97 -0.26 0.68 NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN<br />
5280 RB6556_protein containing DUF1501 3482471 3481050 NaN 0.76 NaN 0.63 1.32 NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN<br />
3170 RB3276_hypothetical protein 1685794 1686438 NaN NaN NaN 0.64 NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN<br />
7204 RB9788_hypothetical protein 5270809 5271042 NaN NaN NaN 0.67 NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN<br />
5050 RB6194_hypothetical protein 3246119 3245988 NaN NaN NaN 0.68 NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Cluster 7<br />
Original row UID START STOP heat_10min heat_20min heat_40min heat_60min heat_300min cold_10min cold_20min cold_40min cold_60min cold_300min salt_10min salt_20min salt_40min salt_60min salt_300min<br />
49 RB10075_hypothetical protein 5435202 5436659 0.48 0.17 0.28 -0.15 -0.32 0.97 1.07 1.01 1.3 0.3 0.66 0.10 NaN -0.04 0.44<br />
6828 RB9157_secreted protein 4899549 4899277 0.42 -0.11 -0.39 -0.06 -0.16 1.52 1.6 1.23 1.31 0.01 0.07 0.10 NaN 0.05 0.42<br />
163 RB10263_hypothetical protein 5546338 5546162 0.69 -0.58 -0.61 -0.58 0.2 1.19 1.54 0.85 1.32 -0.27 1.31 -0.05 0.17 1.02 0.92<br />
509 RB10861_hypothetical protein 5896851 5896991 -0.01 -1 -1.34 -1.15 -0.33 2.6 2.38 1.9 2.14 -0.31 0.54 -0.06 -0.26 0.08 1.46<br />
513 RB10867_hypothetical protein 5899359 5899982 0.59 0.01 -0.38 -0.25 -0.02 1.49 1.5 1.13 1.38 -0.01 -0.10 -0.15 NaN 0.11 0.81<br />
2324 RB1909_Globin 992934 993434 NaN NaN NaN NaN 0.08 1.4 1.44 1.85 1.1 -0.19 0.09 NaN NaN -0.32 2.10<br />
4326 RB5003_Rh-like protein/ammonium transporter 2572662 2574158 -0.2 0.03 -0.01 0.14 0.1 2.11 1.81 1.18 1.39 0.46 0.98 -0.12 -0.33 0.62 1.92<br />
510 RB10862_Rhs family protein 5896997 5898142 0.46 -0.01 -0.13 NaN 0.33 1.57 1.76 1.11 1.32 0.14 0.00 0.04 NaN 0.03 0.67<br />
1063 RB11798_conserved hypothetical protein 6360319 6360450 0.16 -0.04 0.15 -0.03 -0.03 1.06 0.78 1.29 0.8 0.55 0.76 -0.09 NaN -0.07 0.88<br />
3519 RB379_ferredoxin--nitrite reductase 194217 196058 NaN NaN NaN NaN NaN 1.06 1.32 1.19 1.03 -0.04 -0.09 0.16 NaN NaN NaN<br />
4144 RB4742_Universal stress protein 2433589 2432615 0.67 NaN NaN NaN -0.14 0.58 1.67 1.29 0.86 NaN NaN NaN NaN NaN NaN<br />
5757 RB7341_HEMAGGLUTININ/HEMOLYSIN-RELATED PROTEIN 3913925 3936541 0.56 0.15 -0.51 -0.41 -0.46 0.82 2.11 1.12 1.93 0.86 NaN NaN NaN NaN NaN<br />
108 RB10167_hypothetical protein 5497096 5496917 0.31 -0.16 -0.09 -0.1 0.67 0.67 1.39 1.1 1.08 0.41 0.24 NaN NaN 0.25 0.63<br />
1105 RB11862_conserved hypothetical protein, secreted 6387327 6387157 0.34 -0.22 -0.16 -0.09 0.59 0.27 1.29 0.77 1.16 0.05 0.72 -0.13 0.11 0.95 0.15<br />
1113 RB11877_thiophene and furan oxidation protein 6395113 6396510 0.16 -0.03 0.11 -0.12 0.3 1.4 1.35 1.23 1.6 0.67 NaN -0.23 NaN -0.08 0.28<br />
1526 RB12541_thioredoxin 6756939 6758414 NaN 0.03 -0.1 NaN NaN 0.52 1.53 0.69 NaN 0.23 NaN NaN NaN NaN NaN<br />
1681 RB12795_secreted protein 6902552 6904978 0.73 0.04 -0.47 -0.42 1.24 1.23 0.97 0.98 1.41 -0.32 NaN -0.15 -0.58 NaN NaN<br />
2106 RB1517_Lipoprotein releasing system ATP-binding protein lolD 776879 776190 -0.15 0.16 0.09 -0.2 0.47 0.56 1.29 0.77 1.09 0.5 NaN NaN NaN NaN 0.57<br />
3491 RB375_conserved hypothetical protein 189235 190170 -0.21 NaN NaN 0.46 0.28 1.81 2.03 1.68 1.33 0.27 NaN NaN NaN NaN NaN<br />
4145 RB4744_universal stress family protein 2434533 2433604 0.49 0.64 NaN NaN 0.35 1.15 1.31 1.07 0.84 0.11 0.07 NaN NaN -0.24 NaN<br />
6472 RB8530_hypothetical protein 4550886 4550725 0.53 0.07 0.21 -0.23 0.01 1.16 1.57 1.19 1.28 0.3 0.49 0.02 0.12 0.59 1.43<br />
7171 RB973_Lipolytic enzyme, G-D-S-L 511727 509484 0.34 0.1 -0.19 -0.05 0.32 0.76 1.31 0.7 0.76 0.04 0.45 0.26 0.73 0.35 0.57<br />
234 RB10391_membrane protein 5615419 5616087 0.22 0.14 -0.04 0.17 0.72 0.75 1.46 0.95 1.03 0.61 0.36 NaN NaN 0.19 1.06<br />
604 RB11011_conserved hypothetical protein, secreted 5971127 5970582 1.01 0.35 -0.03 -0.34 0.88 0.67 1.35 0.97 1.22 0.47 0.07 -0.31 NaN 0.23 0.79<br />
675 RB11137_hypothetical protein 6041524 6042972 0.56 0.19 0.1 NaN 0.35 1.26 1.27 1 1.31 0.71 0.03 0.07 -0.24 -0.06 0.22<br />
3184 RB3299_protein containing DUF1559 1693917 1692925 0.1 0.42 0.06 0.64 0.37 1.72 1.74 1.27 0.86 0.82 0.62 0.12 0.33 0.38 1.68<br />
6676 RB8881_hypothetical protein 4726480 4726013 0.52 0.43 0.59 0.29 0.06 0.5 0.94 0.94 1.27 0.63 0.51 -0.06 0.34 0.56 0.75<br />
7268 RB9897_hypothetical protein 5333773 5333549 0.45 0.28 0.17 0.64 0.28 0.71 1.21 0.96 1.36 0.48 0.32 -0.18 -0.17 0.21 -0.01<br />
971 RB11636_conserved hypothetical protein 6275447 6275232 0.11 0.01 0.6 -0.11 0.58 0.37 1.54 1.3 1.4 0.88 NaN NaN NaN NaN 0.33<br />
1417 RB12372_RNA polymerase ECF-type sigma factor 6658702 6659430 0.9 -0.36 -0.06 0.37 0.9 0.79 0.53 0.88 0.88 0.09 0.84 0.26 0.36 0.87 0.29<br />
1616 RB12689_secreted protein 6833004 6833324 0.51 0.37 0.54 0.11 0.72 0.66 1.3 1.07 1.06 0.29 0.22 NaN NaN 0.25 0.78<br />
1944 RB13239_conserved hypothetical protein, membrane 7102571 7101981 0.62 -0.02 0.13 -0.07 0.13 1.04 1.39 1.49 2.05 0.63 1.28 0.43 0.03 1.74 0.05<br />
2097 RB1502_two-component response regulator 769609 770010 1.19 -0.21 0.2 0.03 1.27 1.02 0.65 0.64 1.56 0.05 0.35 -0.22 NaN 0.54 0.76<br />
6266 RB8181_Protein of unknown function DUF1355 4375005 4377428 0.33 0.85 -0.44 0.13 1.22 NaN 1.82 1.33 1.47 0.13 NaN NaN NaN NaN -0.19<br />
110 RB10170_cysteine synthase B, O-acetylserine sulfhydrolase B 5497260 5498231 NaN 0.06 0.36 -0.1 0.74 0.37 1.49 1.11 1.07 0.55 NaN NaN NaN NaN 0.49<br />
197 RB10327_Translation initiation factor SUI1 5582627 5583010 0.79 0.52 -0.51 -0.28 0.82 0.79 0.84 1.56 1.09 1.15 NaN NaN NaN NaN NaN<br />
603 RB11010_conserved hypothetical protein, secreted 5970585 5969749 0.97 0.11 -0.01 -0.03 0.87 0.43 1.46 0.97 1.24 0.77 0.12 NaN NaN 0.43 1.05<br />
607 RB11016_protein containing DUF1559 5974211 5973102 1 -0.08 -0.09 0.31 0.83 0.33 0.66 1.16 1.08 0.73 0.27 -0.21 NaN 0.39 0.73<br />
1174 RB11976_DNA-binding response regulator 6455996 6455283 1.26 0.48 0.26 0.07 0.33 0.69 1.23 1.23 1.72 0.58 0.83 0.10 NaN 0.59 0.61<br />
1651 RB12747_conserved hypothetical protein 6870758 6870919 1.41 0.07 0.02 0.08 0.38 NaN NaN 0.48 NaN -0.07 0.71 0.00 NaN 1.13 0.44<br />
2007 RB1352_hypothetical protein 686463 687803 0.32 0.33 0.52 NaN 0.62 0.58 1.26 0.78 1.01 0.69 NaN NaN NaN 0.21 1.04<br />
7146 RB968_protein containing DUF1596 507840 507079 1.25 -0.39 -0.22 -0.33 0.85 0.25 1.02 1.22 1.35 -0.16 1.13 0.03 0.16 1.79 1.25<br />
601 RB11008_secreted protein 5967078 5968262 1.11 -0.01 0.11 0.11 1.14 0.28 0.73 1.21 1.35 0.61 0.13 -0.75 NaN 0.00 0.94<br />
837 RB11396_conserved hypothetical protein 6178445 6178555 1.51 0.41 -0.52 0.44 0.93 1.87 1.39 1.07 1.69 0.12 0.42 -0.02 -0.11 0.83 -0.05<br />
1624 RB12697_conserved hypothetical protein 6837436 6842547 0.85 -0.02 -0.49 0.17 0.65 0.32 1.54 0.7 1.08 0.3 NaN NaN NaN NaN 1.59<br />
1680 RB12790_conserved hypothetical protein 6895351 6902541 0.79 0.63 -0.28 0.21 1.38 0.48 2.23 1.51 1.94 0.32 NaN NaN NaN 0.53 0.84<br />
3891 RB4375_conserved hypothetical protein 2252103 2242933 0.65 0.68 -0.16 -0.14 0.68 0.46 2.45 1.66 2.07 0.54 NaN NaN NaN 0.12 -0.42<br />
5498 RB6912_secreted protein 3672793 3672960 0.72 0.24 0.42 0.64 0.38 3.15 2.71 1.94 2.54 0.75 0.35 0.00 0.12 0.78 2.38<br />
5503 RB6920_protein containing DUF1559 3676847 3677914 1.4 0.05 0.37 0.16 1.02 1.82 2.09 1.76 2.07 0.43 0.56 -0.04 0.05 0.95 1.92<br />
6267 RB8186_secreted protein 4377386 4378576 0.06 0.43 NaN NaN 1.28 1.05 2.06 1.6 1.47 0.53 NaN NaN NaN NaN -0.11<br />
6852 RB9199_hypothetical protein 4918323 4918120 0.4 0.43 0.47 0.99 0.57 1.77 2.12 1.44 1.85 0.85 0.66 0.22 NaN -0.30 1.15<br />
6854 RB9200_4-nitrophenylphosphatase 4919220 4918369 1.21 0.58 0.8 0.57 0.58 2.05 1.84 1.36 1.99 0.33 0.79 -0.03 -0.28 0.36 1.58<br />
1198 RB1201_hypothetical protein 612754 612629 -0.07 0.49 0.39 0.31 0.42 NaN 1.42 0.91 0.92 0.73 0.52 NaN NaN 0.86 1.31<br />
2030 RB1392_RNA polymerase sigma factor 711705 710683 1.02 0.52 0.28 0.2 1.04 1.03 1.06 1.09 2.09 0.16 0.36 -0.24 0.04 -0.41 -0.10<br />
2112 RB1523_hypothetical protein 778654 779130 1.01 0.69 0.39 0.55 1.34 1.4 2.32 1.38 1.49 0.24 0.59 NaN NaN 0.28 1.81<br />
5920 RB7603_outer membrane efflux protein 4091070 4088560 0.67 0.44 0.63 0.35 0.31 0.75 0.83 1.12 1.4 1.53 0.51 NaN NaN 0.21 1.41
6693 RB8904_NAD-dependent epimerase/dehydratase 4740862 4741962 0.71 0.39 0.69 0.37 0.29 0.46 0.9 0.85 1.2 1.2 0.38 NaN NaN 0.35 0.88<br />
208 RB10342_hypothetical protein 5593929 5593621 0.62 0.81 0.67 0.74 0.89 1.29 1.29 1.37 1.12 0.56 0.89 0.85 -0.27 0.88 -0.04<br />
696 RB11174_metallo-beta-lactamase family protein 6065046 6063637 1.27 0.91 0.43 0.57 1.16 1.8 1.65 1.24 1.52 0.28 0.42 0.24 0.39 0.69 1.08<br />
1682 RB12797_conserved hypothetical protein 6905009 6905182 0.99 1.05 -0.34 0.28 1.1 1.47 2.4 1.89 2.17 0.97 -0.37 NaN NaN 0.71 1.37<br />
1972 RB13294_conserved hypothetical protein 7127129 7127677 0.47 0.43 0.56 0.32 0.79 0.45 1.29 0.5 1.39 0.78 NaN NaN NaN 0.38 1.25<br />
2111 RB1522_protein containing DUF1559 777494 778657 1.26 0.87 0.56 0.77 1.1 1.85 1.9 1.6 1.82 0.42 0.60 -0.13 NaN 0.66 1.69<br />
4325 RB5001_hypothetical protein 2572354 2572665 0 0.39 0.91 0.7 0.48 1.64 1.85 1.6 1.13 0.88 NaN 0.49 0.16 1.26 2.62<br />
6262 RB8174_conserved hypothetical protein 4367851 4368960 1.27 0.78 -0.25 0.52 1.5 0.81 1.4 0.97 1.37 0.02 -0.24 0.42 -0.23 -0.09 0.80<br />
6891 RB9268_flagellar protein FlbD family protein 4955675 4955920 NaN 0.38 -0.12 0.39 0.8 NaN NaN NaN NaN 0.2 NaN 0.39 NaN 0.47 NaN<br />
7290 RB9930_hypothetical protein 5349331 5348975 1.43 0.58 0.16 0.57 0.88 0.76 0.98 1.1 0.97 0.64 0.83 NaN NaN 0.12 0.52<br />
537 RB10902_hypothetical protein 5918562 5918819 NaN 0.45 0.15 0.74 0.27 NaN 1.44 1 0.7 0.69 NaN NaN NaN 0.01 NaN<br />
1642 RB12734_conserved hypothetical protein, secreted 6863889 6862186 1.07 0.18 0.43 0.01 1.36 0.49 1.15 0.96 1.21 0.05 0.42 NaN NaN 0.83 1.19<br />
1656 RB12752_HtrA-like serine protease 6871994 6873523 1.14 0.63 0.34 NaN 1.4 0.75 1.87 1.18 1.52 0.67 0.37 NaN NaN 0.07 0.77<br />
2080 RB1477_arylsulfatase precursor 755327 756943 -0.06 0.36 0.39 NaN 1.59 0.9 1.72 1.26 1.13 0.4 NaN NaN NaN NaN 1.62<br />
3413 RB3646_hypothetical protein 1895252 1895407 NaN 0.39 NaN NaN 0.3 NaN 1.54 NaN NaN 1.28 NaN NaN NaN NaN NaN<br />
5010 RB6127_secreted protein 3223592 3219093 NaN 0.57 -0.12 0.19 0.79 NaN 1.3 0.69 1.12 1.56 NaN NaN NaN NaN 0.53<br />
5699 RB7233_hypothetical protein 3840182 3840385 0.57 0.58 0.84 0.75 1.08 1.13 1.32 1.17 1.48 0.8 1.83 0.65 0.15 0.93 0.22<br />
5740 RB7299_ribosomal protein S6 modification protein 3878200 3879417 0.84 0.84 0.44 0.75 0.9 0.75 1.22 0.5 1.14 1.16 0.52 0.27 NaN 0.40 0.60<br />
6607 RB8773_Band 7 protein 4665094 4663364 NaN 0.24 0.04 0.31 NaN NaN NaN NaN NaN 0.54 NaN NaN NaN NaN NaN<br />
639 RB11075_Bacterial regulatory protein, MarR 6005727 6005224 -0.12 0.09 0.75 0.44 1.85 0.23 1.12 0.93 1.11 0.37 0.16 0.05 NaN 0.94 1.03<br />
1016 RB11698_conserved hypothetical protein, secreted 6308550 6307780 0.6 0.18 0.61 0.9 1.2 NaN 0.98 0.88 1.01 0.78 0.52 NaN NaN 0.15 1.88<br />
1106 RB11863_RecA bacterial DNA recombination protein 6388538 6387411 1.18 0.2 0.4 0.83 0.81 0.5 1.21 0.61 1.21 0.87 0.97 -0.20 NaN 0.43 0.92<br />
1670 RB12773_twitching motility protein PilT 6884826 6885932 0.69 -0.52 0.41 0.12 0.8 0.69 1.13 1.22 1.4 1 1.26 0.08 0.84 1.15 1.17<br />
755 RB11269_CYTOCHROME C SIGNAL PEPTIDE PROTEIN 6109738 6110583 0.5 0.48 0.35 0.43 1.11 NaN 1.4 0.79 1.07 0.7 1.69 0.55 0.15 1.45 0.38<br />
886 RB11484_conserved hypothetical protein 6212742 6212611 1.12 0.43 0.46 0.31 0.64 0.5 0.83 0.68 1.16 0.2 0.64 0.31 NaN 0.69 1.12<br />
4423 RB5167_hypothetical protein 2658500 2658366 0.75 0.91 -0.02 1.37 1.23 NaN 1.75 1.23 NaN 0.91 0.75 NaN NaN 0.74 0.64<br />
5246 RB6500_Beta-ketoacyl synthase 3454800 3443803 NaN NaN -0.28 -0.06 NaN NaN NaN NaN NaN 1.03 1.09 NaN NaN NaN NaN<br />
5274 RB6549_membrane protein 3477191 3478189 NaN 0.18 0.64 0.57 0.1 NaN 1.34 0.45 1.16 1.3 NaN NaN NaN NaN 0.60<br />
2363 RB1994_hypothetical protein 1050487 1051065 NaN 0.33 0.41 NaN 0.51 NaN NaN NaN NaN 0.76 NaN NaN NaN NaN NaN<br />
1447 RB12416_secreted protein 6679578 6680456 0.72 0 0.24 0.18 0.21 0.75 1.09 1.23 1.18 1.19 1.42 NaN NaN 1.00 1.24<br />
625 RB11050_Na+/Pi-cotransporter family protein 5991910 5990726 NaN 0.07 0.37 0.3 NaN NaN NaN NaN NaN 0.89 NaN NaN NaN NaN NaN<br />
6248 RB8149_Carbon storage regulator 4355339 4355551 1.18 0.73 0.98 0.72 0.78 1.11 1.14 1.15 1.09 0.71 0.51 -0.17 NaN 0.39 0.34<br />
1749 RB12897_secreted protein 6947028 6946117 NaN 0.62 0.5 0.18 0.38 NaN NaN NaN NaN 0.75 NaN NaN NaN NaN NaN<br />
6453 RB8504_hypothetical protein 4536996 4536661 NaN 0.56 0.76 0.9 0.63 0.48 1.85 0.94 1.29 0.96 0.86 NaN NaN 0.06 0.09<br />
5700 RB7235_secreted protein 3840505 3841299 0.48 0.79 0.92 0.85 1.38 NaN 1.62 1.2 1.37 1.44 1.67 0.19 NaN 0.70 0.01<br />
6536 RB8639_conserved hypothetical protein, secreted 4604495 4605874 NaN 0.32 0.68 0.17 1.03 NaN 1.21 0.72 0.89 0.37 1.16 NaN NaN 0.73 0.75<br />
1851 RB13092_conserved hypothetical protein 7025003 7025143 0.26 -0.13 0.38 NaN 0.43 0.71 1.33 0.62 1.1 0.07 -0.22 -0.21 NaN 0.63 NaN<br />
6887 RB9260_secreted protein 4951242 4952522 0.38 0.9 0.56 0.48 0.16 1.26 1.32 1 1.05 0.49 0.20 -0.18 -0.39 -0.45 -0.02<br />
286 RB10478_conserved hypothetical protein, secreted 5681878 5682843 NaN 0.43 0.3 0.21 0.39 NaN NaN NaN NaN 0.52 NaN NaN NaN NaN NaN<br />
24 RB10033_hypothetical protein 5409271 5408291 0.92 0.47 0.03 0.6 0.87 0.87 1.02 0.94 1.31 0.46 0.43 -0.21 -0.18 0.11 -0.19<br />
1012 RB11687_conserved hypothetical protein 6301412 6301528 NaN NaN NaN 0.23 NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN<br />
5079 RB6244_hypothetical protein 3278979 3278695 NaN NaN NaN 0.24 NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN<br />
581 RB10978_Small multidrug resistance protein 5947515 5947883 1.22 0.87 0.68 0.53 0.99 0.71 1.38 1.01 0.84 0.9 0.37 0.34 0.14 0.36 -0.06<br />
6305 RB8247_hypothetical protein 4409386 4409183 NaN 0.39 0.42 NaN 0.65 NaN NaN NaN NaN 0.44 NaN NaN NaN NaN NaN<br />
1072 RB11807_secreted protein containing DUF1555 6363652 6364356 0.76 0.17 0.43 -0.32 0.15 0.67 0.88 0.61 1.21 0.01 0.47 -0.27 NaN 0.09 0.45
Cluster 15<br />
Original row UID START STOP heat_10min heat_20min heat_40min heat_60min heat_300min cold_10min cold_20min cold_40min cold_60min cold_300min salt_10min salt_20min salt_40min salt_60min salt_300min<br />
3954 RB4464_secreted protein 2292075 2291404 0.48 0.17 -0.18 -0.66 0.14 -0.04 -0.16 0.16 0.15 0.44 -0.79 -0.58 -0.08 -1.16 -1.05<br />
6268 RB8188_hydroxypyruvate isomerase 4378573 4379352 0.27 0.26 -0.42 -0.42 0.07 -0.91 -0.74 -0.13 0.07 0.4 -1.14 -0.36 NaN -1.21 -1.16<br />
6498 RB8565_Chain A, 1.8 A Crystal Structure Of Ioli Protein With A Binding Zinc Atom 4573365 4572448 -0.46 -0.09 -0.28 -0.24 -0.32 -0.57 -0.54 -0.14 -0.16 0.57 NaN NaN NaN -1.57 -0.76<br />
261 RB10436_glycosyl transferase, group 1 family protein 5654064 5652865 0.79 0.09 -0.14 -0.02 0.17 0.08 0.09 0.09 0.12 -0.08 -0.67 -0.56 -0.45 -1.47 -1.09<br />
1602 RB12664_conserved hypothetical protein, secreted 6817708 6818412 0.31 -0.07 -0.07 -0.12 -0.14 -0.48 -0.29 0.08 0.11 0.05 -0.99 NaN NaN -1.03 -0.67<br />
1662 RB12760_conserved hypothetical protein 6876908 6877060 0.33 0.2 0.55 0.51 0.59 -0.98 -0.85 -0.21 -0.54 0.15 -1.22 NaN NaN -1.80 -0.95<br />
1702 RB12823_tRNA (guanine-N1-)-methyltransferase 6917306 6916578 0.09 0.05 0.32 -0.24 0.01 -0.96 -0.33 -0.11 -0.12 0.12 NaN NaN NaN -1.41 -0.73<br />
3864 RB4333_glycosyl transferase, group 1 family protein 2230615 2231700 0.89 0.67 0.1 0.03 -0.46 0.24 -0.01 0.02 -0.16 0.46 -0.72 -0.70 -0.22 -1.12 -1.29<br />
3898 RB4386_CYSTEINE SYNTHASE A 2259814 2258765 0.75 0.03 -0.42 -0.58 -0.01 0.2 -0.04 0.15 0.24 0.66 -1.02 -0.83 -0.66 -1.54 -1.52<br />
5358 RB6690_fructose-bisphosphate aldolase 3555887 3556918 -0.6 -0.78 -0.11 -0.77 -0.57 -0.5 -0.28 0.14 0.02 0.86 -1.18 NaN NaN -1.40 -0.27<br />
5677 RB7203_hypothetical protein 3819614 3820057 -0.67 -0.73 -0.44 -0.71 -0.62 -0.1 0 0.1 0.31 0.22 -0.69 NaN NaN -1.24 -0.35<br />
6055 RB7818_Ribosomal protein S12/S23 4185730 4186095 -0.64 -0.49 -1.25 -1.21 -0.63 0.01 0.14 0.4 0.31 0.07 -0.88 -1.50 -2.00 -1.68 -1.49<br />
6057 RB7820_Ribosomal protein S7 4186170 4186643 -0.67 -0.53 -0.69 -0.88 -0.63 0.6 0.44 0.57 0.59 0.81 -0.69 -1.69 NaN -1.76 -1.66<br />
6069 RB7833_Ribosomal protein L3 4192692 4193378 -0.55 -0.41 -0.61 -0.53 -0.52 -0.26 -0.24 -0.3 -0.1 -0.17 -1.28 -2.01 NaN -1.81 -1.31<br />
6245 RB8143_hypothetical protein 4354179 4353961 0.33 -0.59 -0.51 -0.3 -0.58 0.5 0.2 0.32 0.4 0.49 -0.53 -0.43 0.08 -0.60 -1.63<br />
6246 RB8144_hypothetical protein 4354622 4354191 -0.05 -0.42 -1.51 -1.44 -1.02 -0.19 0.04 0.12 0.15 0.47 -2.43 -1.74 -0.76 -2.22 -1.89<br />
6269 RB8189_ATP-dependent RNA helicase DeaD 4382134 4379339 0.45 0.34 -0.48 -0.74 0.18 -0.39 -0.41 -0.29 -0.05 -0.16 -0.79 NaN -0.78 -1.64 -1.77<br />
6580 RB8725_50S ribosomal protein L33 4638301 4638465 -0.45 -0.46 0.17 -0.52 -0.82 -0.6 -0.39 -0.03 -0.28 0.06 -1.15 -1.08 NaN -1.79 -0.97<br />
6911 RB9304_Ribosomal protein L31 4974378 4974623 -0.35 -0.46 -0.21 -0.51 -0.66 -0.03 -0.36 -0.3 -0.39 0.04 -0.87 -1.04 NaN -0.95 -0.82<br />
7285 RB9922_Peptidyl-tRNA hydrolase 5345441 5344869 -0.29 -0.29 -0.09 -0.22 -0.44 -0.47 -0.33 -0.28 -0.16 -0.15 -0.43 -0.69 -0.51 -1.22 -1.29<br />
88 RB10134_Ribosomal protein L21 5469243 5468929 -0.48 -0.32 -0.19 -0.23 -0.86 0.36 -0.2 -0.08 -0.02 -0.2 -0.93 -0.85 NaN -1.39 -1.36<br />
281 RB10469_30S ribosomal protein S9 5678261 5677848 -0.67 -0.65 -0.39 -0.55 -0.89 -0.19 -0.43 -0.17 -0.11 0.19 -0.97 NaN NaN -1.41 -0.88<br />
378 RB10640_Elongation factor Ts 5774380 5775360 0.08 -0.66 -0.42 -0.74 -0.43 -0.53 -0.4 -0.11 -0.29 0.06 -0.82 NaN NaN -1.15 -0.20<br />
522 RB10880_2-methylthioadenine synthetase 5907340 5905907 -0.54 -0.23 -0.4 -0.62 -0.84 -0.31 -0.34 -0.3 -0.11 -0.09 -0.70 -0.59 NaN -1.20 -0.34<br />
577 RB1097_membrane protein 570281 569067 0.83 -0.28 -0.28 -0.03 -0.08 -0.09 0.08 0.3 0.39 0.13 -1.20 -1.31 NaN -1.93 -1.80<br />
597 RB1100_capsule biosynthesis protein CapC 570778 570278 0.46 0 -0.22 -0.12 -0.36 0.07 0.15 0.18 0.32 0.13 -1.33 -1.01 NaN -2.09 -2.91<br />
613 RB1103_capsule biosynthesis protein 572105 570780 1 -0.31 -0.3 -0.65 0.07 0.48 0.57 0.51 0.69 0.21 -1.08 NaN NaN -1.96 -2.52<br />
2350 RB1964_hypothetical protein 1034475 1035977 -0.67 -0.15 0.03 -0.23 -0.67 -0.64 -0.86 -0.57 -0.72 0.26 -1.19 NaN NaN -1.35 -0.22<br />
2645 RB2436_hypothetical protein 1268971 1267766 -0.62 -0.23 -0.01 -0.62 -0.85 -0.29 -0.48 0 -0.19 0.53 -0.32 -1.04 0.03 -0.86 -0.61<br />
2986 RB2970_Phosphatidylethanolamine N-methyltransferase 1530415 1531200 -0.78 -0.14 -0.5 -0.64 -0.69 -0.09 -0.35 -0.02 -0.06 0.56 -0.75 NaN -0.36 -1.24 -1.30<br />
4065 RB4633_SecD/SecF/SecDF export membrane protein 2371842 2375180 -0.01 -0.12 NaN NaN -0.48 -0.53 0.02 -0.15 -0.25 0.29 NaN NaN NaN NaN NaN<br />
4579 RB5411_hypothetical protein 2794516 2794623 -0.82 -0.35 -0.08 -0.16 -0.91 -0.24 -0.35 -0.54 -0.4 -0.4 -0.47 -0.93 -0.10 -1.24 -0.56<br />
4749 RB5681_Trigger factor 2965080 2966843 -0.08 -0.26 NaN -0.27 -0.36 -0.68 0.1 -0.27 -0.19 0.36 NaN NaN NaN NaN NaN<br />
4824 RB5801_Ribosomal protein S15 3039583 3039852 -0.64 -0.65 -0.44 -0.4 -0.74 -0.13 -0.25 -0.26 -0.16 -0.19 -0.49 NaN NaN -1.48 -0.46<br />
5102 RB6285_S-adenosyl-L-homocysteine hydrolase 3304945 3303599 -0.33 -0.06 0.24 0.04 -0.18 -0.59 -0.82 -0.52 -0.7 0.06 NaN NaN -0.10 -1.52 -0.85<br />
5709 RB7247_glutamine synthetase II 3850538 3851638 0.01 -0.63 -0.22 -0.73 -0.7 -0.54 -0.7 -0.51 -0.5 -0.22 -1.27 NaN NaN -1.90 0.27<br />
6036 RB778_secreted protein containing DUF1559 418773 417532 1.12 0.23 0.93 0.65 0.36 -0.43 0 0.03 -0.23 0.15 -1.18 NaN NaN -1.41 -1.95<br />
6102 RB7897_Protein secE/sec61-gamma protein 4218945 4219403 0.16 -0.28 -0.05 -0.4 -0.45 -1.34 -1.06 -0.62 -0.76 0.07 NaN NaN NaN -1.67 -1.20<br />
6432 RB8469_lysine/ornithine decarboxylase 4520779 4521975 0.05 -0.63 -0.28 -0.43 -0.65 NaN -0.35 -0.6 -0.81 0.07 NaN NaN NaN -1.22 NaN<br />
6507 RB8584_Nitrogen regulatory IIA protein 4577819 4578298 -0.88 -0.43 -0.66 -0.55 -1.07 -0.04 -0.49 -0.16 -0.04 0.11 -0.55 -0.53 -0.49 -1.15 -0.44<br />
258 RB10431_conserved hypothetical protein 5650397 5649426 -0.25 -0.82 -1.1 -1.59 -0.68 -0.48 -0.55 -0.14 -0.09 -0.81 -1.22 -1.14 -0.31 -1.90 -1.95<br />
1576 RB12626_DNA-directed RNA polymerase alpha chain 6797797 6798792 -0.6 -0.81 -0.65 -0.05 -1.1 -0.02 -0.62 -0.23 -0.18 -0.28 -0.80 -0.66 -0.42 -1.56 -1.02<br />
1577 RB12628_50S ribosomal protein L17 6798878 6799510 NaN -0.78 -0.57 -0.62 -1.44 NaN -0.68 -0.28 -0.33 -0.06 NaN -1.28 NaN -1.83 -1.22<br />
2118 RB1533_conserved hypothetical protein 787091 785889 NaN -0.35 -0.39 NaN -0.35 NaN NaN -0.17 -0.08 -0.03 NaN NaN NaN NaN NaN<br />
2782 RB264_Ribosomal protein S21 132677 132871 -1.03 -0.63 -0.28 -0.65 -1.35 -0.19 -0.58 -0.4 -0.36 0.4 -0.71 NaN NaN -1.82 -1.44<br />
4286 RB4954_secreted protein 2547861 2548361 -0.18 -0.18 0.17 -0.17 -0.33 -1.53 -1.5 -0.9 -1.29 -0.03 NaN NaN NaN -1.91 -1.17<br />
5561 RB7022_Ribosomal protein S20 3735291 3735563 -0.93 -0.85 -0.48 -0.77 -1.45 -0.41 -0.38 -0.34 -0.15 0.16 -1.13 NaN -0.33 -2.08 -1.44<br />
6368 RB8356_hypothetical protein 4459802 4460011 -0.78 -0.81 -0.78 -0.8 -0.42 -0.39 -0.37 -0.15 -0.39 -0.38 -0.82 NaN NaN -1.49 -0.91<br />
139 RB10219_ATP synthase epsilon chain 5521003 5521389 0.07 -0.67 -0.64 NaN -1.03 NaN -0.48 -0.42 -0.6 -0.64 -0.98 -1.02 NaN NaN -0.84<br />
280 RB10468_Translation initiation factor IF-1 5677835 5677614 -0.34 -0.71 -0.19 -0.56 -0.96 -0.74 -0.74 -0.42 -0.62 0.12 NaN NaN NaN -1.36 -0.85<br />
1244 RB12087_Dihydroxy-acid and 6-phosphogluconate dehydratase 6520558 6522321 NaN NaN NaN NaN -0.48 NaN NaN NaN NaN 0.46 NaN NaN NaN NaN NaN<br />
1714 RB12840_50S ribosomal protein L10 6922785 6923309 -0.14 -0.43 -0.42 0.02 -0.63 NaN -0.83 NaN NaN 0.63 NaN NaN NaN NaN NaN<br />
2902 RB2840_RNA polymerase sigma factor 1473891 1474409 NaN NaN NaN NaN NaN NaN -0.11 -0.33 -0.28 0.3 NaN NaN NaN NaN NaN<br />
4639 RB5500_protein containing DUF28 2845759 2845019 NaN NaN NaN -0.41 -0.38 NaN -0.25 -0.28 -0.47 0.77 NaN NaN NaN NaN NaN<br />
6088 RB7863_Ribosomal protein L15 4202048 4202542 -0.62 -0.82 -1 NaN -1.29 NaN -0.59 -0.38 -0.51 0.73 NaN NaN NaN NaN -1.05<br />
6164 RB8001_hypothetical protein 4277541 4276849 NaN -0.3 -0.37 -0.47 NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
6509 RB8590_hypothetical protein 4578825 4579148 -0.44 -0.56 -0.57 -0.73 -0.7 -0.83 -1.07 -0.38 -0.48 -0.19 -0.92 -0.56 0.04 -1.28 -0.49<br />
4572 RB5403_hypothetical protein 2790360 2790208 NaN NaN NaN NaN NaN NaN -0.32 NaN NaN NaN NaN NaN NaN NaN NaN<br />
4658 RB5540_LmbE-like protein 2882665 2883432 NaN NaN NaN NaN NaN NaN -0.34 NaN NaN NaN NaN NaN NaN NaN NaN<br />
4755 RB5694_Dethiobiotin synthetase 2971417 2970632 NaN NaN NaN NaN NaN NaN -0.36 -0.26 -0.33 0.1 NaN NaN NaN NaN NaN<br />
6948 RB9356_protein containing DUF1579 5012771 5012382 -0.59 -0.58 -1.84 -1.73 -0.57 0.25 0.79 0.7 0.44 0.08 -0.48 NaN NaN -1.90 -0.72<br />
4041 RB4598_membrane protein 2355806 2356726 NaN NaN NaN NaN NaN NaN -0.11 -0.2 -0.39 0.2 NaN NaN NaN NaN NaN<br />
3640 RB3972_hypothetical protein 2056175 2056288 NaN -0.32 NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN<br />
5250 RB6505_hypothetical protein 3456265 3456110 NaN -0.36 -0.35 NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN<br />
6941 RB9348_hypothetical protein 5008826 5008536 NaN -0.31 NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN<br />
136 RB10215_ATP synthase alpha chain 5516975 5518498 0.08 -0.54 -0.41 -0.75 -0.77 NaN -0.61 -0.56 -0.6 -0.39 -0.82 -0.43 -0.41 -0.87 -0.88<br />
7252 RB9871_Acetolactate synthase small subunit 5316629 5316045 0.11 -0.28 0.02 -0.49 -0.34 NaN -0.1 -0.38 -0.1 0 -0.56 NaN -0.45 NaN -0.53<br />
5162 RB638_PQQ enzyme repeat domain protein 346447 347757 NaN NaN NaN -0.52 NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN<br />
5218 RB6462_hypothetical protein 3414908 3415180 -0.22 -0.55 -0.52 -0.53 -1.08 -0.69 -0.81 -0.55 -0.47 -0.1 -0.73 -0.44 -0.42 -1.06 -0.99<br />
6116 RB7913_hypothetical protein 4228083 4227877 NaN -0.27 -0.43 -0.55 NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN<br />
260 RB10434_glycosyl transferase, group 1 family protein 5652902 5651763 1.07 -0.24 -0.73 -0.67 0.64 0.14 0.09 0.12 0.13 -0.93 -0.87 -0.55 NaN -1.32 -0.86<br />
1284 RB12160_thioredoxin 6563104 6562778 0.18 -0.61 -0.99 -0.76 -0.69 0.07 0.2 -0.14 0.29 0.26 -0.35 -0.54 0.29 -0.88 -1.12<br />
4355 RB5055_ABC transporter, ATP-binding protein 2601971 2600094 -0.46 -0.48 -0.55 -0.56 -0.61 NaN -0.41 -0.39 -0.49 0.22 NaN NaN NaN NaN NaN<br />
3724 RB4113_transposase 2117681 2116503 NaN -0.23 -0.59 -0.24 NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN<br />
4154 RB4756_conserved hypothetical protein 2437784 2438053 0.05 -0.38 NaN NaN -0.68 NaN 0.05 -0.5 NaN NaN NaN NaN NaN NaN NaN<br />
189 RB10310_NADH dehydrogenase I chain M 5572980 5571523 0.14 -0.07 -0.7 -1 -0.65 -0.02 -0.06 0.12 0.34 0.4 -0.32 -0.37 -0.63 NaN -0.78<br />
4607 RB5450_hypothetical protein 2824513 2824635 0.08 -0.12 -0.17 -0.13 -0.26 0.03 -0.29 -0.27 -0.25 -0.19 -0.41 NaN -0.66 -0.67 -1.04<br />
4598 RB5437_Purine phosphorylase, family 2 2818164 2819081 -0.22 -0.13 -0.13 NaN -0.2 NaN -0.61 -0.34 -0.5 -0.18 -0.70 -0.47 NaN NaN NaN<br />
1939 RB13231_conserved hypothetical protein 7098472 7099530 0.54 -0.26 -0.44 -0.6 -0.52 NaN -0.42 -0.61 NaN -0.42 NaN NaN NaN NaN NaN<br />
134 RB10211_H+-transporting two-sector ATPase, B/B' subunit 5515421 5516206 0.56 0.23 NaN NaN -0.53 NaN 0.15 -0.45 -0.31 0.01 NaN NaN NaN NaN NaN<br />
1973 RB13297_hypothetical protein 7128034 7128630 NaN NaN NaN NaN NaN NaN NaN NaN NaN 0.11 NaN NaN NaN NaN NaN<br />
6360 RB8343_Adenylosuccinate lyase 4454677 4456134 NaN -0.17 -0.19 -0.35 -0.47 NaN -0.26 -0.26 -0.28 0.03 NaN NaN NaN NaN NaN
Cluster 22<br />
Original row UID START STOP heat_10min heat_20min heat_40min heat_60min heat_300min cold_10min cold_20min cold_40min cold_60min cold_300min salt_10min salt_20min salt_40min salt_60min salt_300min<br />
1814 RB13025_monooxygenase 6997019 6996438 1.09 -0.04 0.31 -0.05 1.05 0.59 1.44 0.74 1.23 -0.26 0.92 NaN NaN 1.58 1.20<br />
1872 RB13118_two-component system response regulator 7037043 7037675 1.35 -0.18 -0.32 -1.18 1.33 0.53 1.35 0.94 1.28 0.9 0.75 0.05 2.01 0.95 0.10<br />
1955 RB13259_conserved hypothetical protein 7108365 7106461 NaN 0.25 0.5 -0.18 0.7 NaN NaN 0.53 NaN 0.12 0.62 NaN NaN 1.95 0.10<br />
2215 RB1716_protein containing DUF1589 895805 895557 0.75 0.11 0.53 0.67 0.25 NaN NaN 0.47 0.59 0.39 1.92 -0.23 NaN 1.33 1.28<br />
2543 RB2283_protein containing DUF1590 1197719 1197102 0.88 -0.03 0.13 0.42 0.57 -0.4 0.85 0.92 0.71 0.62 1.24 NaN 1.03 1.08 2.03<br />
2918 RB2867_hypothetical protein 1491909 1494002 NaN 0.21 0.03 0.22 1.5 NaN 1.18 0.4 0.79 0.23 NaN NaN NaN NaN 1.91<br />
3322 RB3505_secreted protein containing DUF1586 1816597 1816704 1.11 0.49 -0.8 -0.37 0.04 0.17 0.64 0.75 0.42 0.06 1.02 0.57 1.72 1.50 0.91<br />
5038 RB6171_site-specific recombinase 3239091 3238048 0.76 0.14 0.37 0.21 0.5 NaN 1.05 0.71 0.86 0.96 0.95 NaN NaN 1.22 1.47<br />
5779 RB7390_hypothetical protein 3954342 3954968 0.81 0.26 0.59 0.3 0.73 NaN 1.01 0.61 0.81 0.97 0.50 NaN NaN 1.08 1.77<br />
6805 RB9114_hypothetical protein 4876598 4878172 NaN 0.55 0.47 0.28 0.63 NaN 0.79 0.59 0.58 0.51 NaN 0.63 NaN NaN 1.02<br />
6846 RB9191_secreted protein containing DUF1586 4914516 4914653 1.39 0.28 0.3 0.6 0.61 0.46 1.06 1.09 1.38 0.51 0.79 NaN NaN 1.31 0.93<br />
6939 RB9346_hypothetical protein 5008335 5008550 0.97 -0.29 0.09 -0.08 1.01 NaN 0.75 NaN 1.14 0.71 1.25 NaN 1.17 1.87 1.93<br />
205 RB10338_TPR domain protein 5591839 5590379 0.68 -0.21 -0.24 -0.14 0.7 0.55 0.79 0.52 1.17 -0.74 0.73 0.07 2.52 0.56 0.79<br />
209 RB10343_hypothetical protein 5593898 5594059 0.61 0.45 0.24 0.15 0.73 0.87 0.95 0.97 0.73 -0.38 1.10 1.73 1.79 2.10 0.72<br />
226 RB10374_Lipoprotein signal peptidase 5609916 5609188 -0.02 0.16 0.58 0.21 0.36 NaN 1.24 0.81 1.22 0.59 1.04 0.63 1.38 0.93 0.43<br />
531 RB10893_hypothetical protein 5914894 5915067 0.68 -0.17 -0.35 0.21 0.9 0.47 1.16 0.57 0.71 -0.09 0.72 0.49 1.68 1.43 0.69<br />
533 RB10895_hypothetical protein 5916348 5917151 0.87 0.31 0.1 0.24 0.17 1.13 1.29 1.14 1.16 0.34 0.93 1.19 2.41 2.03 0.98<br />
572 RB10960_conserved hypothetical protein, membrane 5941602 5942021 1.32 0.83 0.28 0.37 0.84 0.61 1.11 0.62 1.05 0.56 1.09 1.05 0.83 1.15 0.01<br />
573 RB10965_conserved hypothetical protein, membrane 5942116 5942730 1.52 0.46 -0.01 0.38 1.24 NaN 1.08 0.32 0.39 0.21 1.28 0.74 0.78 1.74 0.41<br />
636 RB11070_hypothetical protein 6000321 6000479 1.39 0.67 0.25 0.39 1.07 0.15 0.85 1.11 0.55 0.29 1.28 0.92 1.71 1.28 -0.37<br />
695 RB11173_conserved hypothetical protein, secreted 6063194 6063586 1.2 0.1 -0.06 -0.18 1.02 0.05 0.95 0.56 0.77 -0.12 0.90 0.58 1.65 1.98 1.56<br />
754 RB11268_hypothetical protein 6109768 6109475 0.44 0.4 0.29 0.46 0.41 NaN 1.09 0.65 0.33 0.19 1.56 1.08 1.55 1.41 0.11<br />
825 RB1138_conserved hypothetical protein 585492 585959 0.56 0.38 0.74 0.62 1.27 NaN 0.73 0.46 0.44 0.26 NaN NaN NaN NaN 1.54<br />
1231 RB12068_serine/threonine protein kinase 6512255 6510594 1.47 0.14 -0.03 -0.38 0.45 0.53 1.1 1.14 1.18 0.74 1.12 1.12 3.72 2.41 0.52<br />
1355 RB12279_MscS Mechanosensitive ion channel 6606558 6605545 1.14 0.43 0.22 0.89 1.38 NaN NaN 0.3 NaN 0.4 0.87 0.59 2.22 2.70 0.75<br />
1366 RB12295_conserved hypothetical protein 6614190 6614450 1.22 0.66 0.38 0.84 1.22 NaN NaN 0.41 NaN 0.38 NaN NaN NaN 1.32 NaN<br />
1646 RB12740_gluconolactonase [precursor] 6865551 6864364 NaN 0.39 0.68 0.89 1.37 NaN 1.19 0.38 0.47 0.44 NaN NaN NaN NaN 1.12<br />
1741 RB12882_conserved hypothetical protein 6940549 6941553 0.76 0.45 1.21 0.68 0.65 NaN 1.18 0.66 0.81 0.68 NaN NaN NaN NaN 0.66<br />
1776 RB12959_conserved hypothetical protein 6974205 6973882 0.38 0.18 0.27 0.3 1.22 NaN NaN 0.42 1.24 0.66 0.73 NaN NaN 1.20 1.73<br />
1867 RB13112_Hybrid sensor histidine kinase 7036128 7034035 1.07 0.71 0.16 0.82 1.39 NaN 0.84 0.2 -0.08 0.4 NaN NaN NaN 1.63 1.31<br />
1869 RB13115_conserved hypothetical protein 7036614 7036369 1.63 0.36 0.53 0.34 1.55 NaN 1.05 0.67 0.59 -0.01 0.92 0.49 2.87 2.00 0.46<br />
2116 RB1531_membrane protein 782534 783889 1.02 0.43 0.74 0.95 0.49 0.17 1.12 0.39 1.24 0.39 0.50 NaN NaN 0.93 1.29<br />
2407 RB2059_NAD-dependent epimerase/dehydratase 1084172 1082949 0.23 0.31 0.82 1.17 0.88 0.6 1.13 1.08 0.57 0.97 2.17 1.07 1.14 1.56 0.91<br />
3971 RB4489_hypothetical protein 2301387 2301905 0.58 0.21 -0.14 -0.04 0.44 0.15 0.15 0.6 0.33 1.2 0.45 0.66 2.36 1.33 0.01<br />
4875 RB5887_integrase 3087343 3086465 0.63 0.42 0.31 0.7 0.26 0.27 1.21 1.56 0.8 1.03 1.49 NaN NaN 1.27 1.58<br />
5098 RB6278_Putative Ig 3302303 3297678 1 0.69 0.44 0.56 1.22 0.06 0.79 0.89 0.54 0.47 NaN NaN NaN NaN 2.20<br />
5365 RB670_hypothetical protein 359315 358905 0.56 0.14 -0.57 -0.65 0.48 0.08 -0.03 0.26 0.46 0.03 0.81 0.77 2.17 2.40 0.72<br />
5496 RB6910_hypothetical protein 3671484 3671356 0.3 -0.11 0.55 0.28 0.64 0.83 0.95 0.85 1.42 -0.33 0.52 0.33 1.46 2.47 3.33<br />
5505 RB6925_hypothetical protein 3678461 3677976 0.97 0.07 -0.39 0.65 0.28 2.01 2.02 2.19 1.8 0.72 1.22 0.85 1.67 1.28 2.17<br />
6242 RB8138_hypothetical protein 4351960 4352187 0.61 0.37 0.68 0.14 1.01 NaN 0.68 0.85 0.64 0.38 1.49 0.73 2.08 1.73 1.26<br />
6343 RB8318_membrane protein 4442318 4442037 0.88 0.65 0.82 0.68 0.84 0.2 1.24 0.59 0.98 0.85 0.92 NaN NaN 1.25 0.69<br />
159 RB10255_MscS Mechanosensitive ion channel 5542656 5543669 NaN -0.27 0.12 -0.03 0.34 NaN 0 NaN NaN -0.21 1.39 1.04 2.47 2.65 1.56<br />
160 RB10256_hypothetical protein 5543753 5544559 0.16 0.14 0.2 -0.36 0.06 0.2 0.53 0.6 0.53 0.3 1.09 0.84 2.51 0.84 0.68<br />
161 RB10258_Survival protein SurE 5544584 5545453 0.99 0.2 -0.14 -0.21 0.19 NaN 0.66 0.67 0.8 0.28 2.49 2.66 4.50 2.77 1.22<br />
195 RB10324_hypothetical protein 5580658 5580846 0.15 0.34 0.47 0.06 0.75 0.46 1.19 1.53 1.57 1.4 1.55 1.11 0.92 1.24 0.89<br />
207 RB10341_hypothetical protein 5593416 5593601 0.87 0.6 0.52 0.52 0.83 0.73 0.85 1.21 1.03 0.4 1.16 0.90 0.90 1.71 0.44<br />
1428 RB12388_ISXo8 transposase 6667206 6665905 0.59 0.82 0.96 0.88 1.3 NaN 1.35 1.15 1.27 0.46 1.13 NaN NaN 1.92 2.25<br />
1674 RB12782_conserved hypothetical protein 6889986 6889819 NaN 0.67 0.01 1.25 1.27 NaN 0.28 0.24 NaN 0.61 NaN NaN NaN 2.17 2.38<br />
1769 RB12941_integrase 6966539 6967417 1.19 1.08 1.2 0.89 0.72 0.05 0.65 1.09 1.01 1.02 0.90 NaN NaN 1.08 1.15<br />
1911 RB13189_ISXo8 transposase 7081765 7083066 0.25 0.81 1.14 1.34 0.56 NaN 1.49 0.67 0.96 0.75 NaN NaN NaN 1.43 NaN<br />
1943 RB13238_TadE-like 7101410 7101904 0 0.22 0.18 0.32 0.35 NaN NaN NaN NaN 0.6 2.00 NaN NaN 3.52 NaN<br />
2032 RB1395_secreted protein 712097 713056 2.1 0.41 -0.12 0.04 2.42 0.97 1.24 1.78 1.84 0.76 1.19 -0.12 1.36 1.47 1.30<br />
2165 RB1617_hypothetical protein 834021 833623 1.18 0.96 1.06 0.89 1.14 0.54 0.98 1.06 0.86 0.45 1.04 0.74 1.55 1.58 1.13<br />
2705 RB2511_hypothetical protein 1313530 1313667 NaN 0.57 0.41 0.68 0.82 NaN 1.28 0.47 NaN 0.55 NaN NaN NaN NaN NaN<br />
2804 RB2679_hypothetical protein 1391557 1392078 0.5 1.15 0.37 0.74 0.69 0.19 0.31 0.54 0.04 0.54 0.74 0.49 2.33 1.63 -0.03<br />
3039 RB3052_hypothetical protein 1577799 1577692 0.93 0.94 1.18 1.29 0.63 0.4 1.5 1.37 1.06 0.61 1.20 0.52 1.48 1.51 1.57<br />
3096 RB3155_hypothetical protein 1627430 1627149 0.35 0.91 1.46 0.93 0.79 NaN 1.06 0.75 0.58 0.8 NaN NaN NaN 1.27 2.69
3725 RB4114_protein containing DUF1582 2117690 2117971 1.25 0.91 1.2 1.09 0.4 -0.22 0.54 0.85 0.74 1.05 1.13 NaN NaN 0.95 1.76<br />
3923 RB4420_DedA family protein 2274093 2273473 0.49 1.27 1.09 0.97 1.43 0.51 0.49 0.56 0.15 1.29 0.92 0.93 1.79 1.04 0.12<br />
3968 RB4484_Peptidase M20 2297267 2298565 0.61 0.67 0.17 0.5 0.29 0.88 0.54 0.35 0.43 0.63 1.29 1.70 2.50 1.36 0.38<br />
3969 RB4485_Glutamate--cysteine ligase, GCS2 2298652 2299770 0.23 1.2 0.01 1 0.2 0.72 0.69 0.44 0.12 0.6 1.24 1.31 1.89 1.33 0.54<br />
3987 RB4510_hypothetical protein 2307657 2307508 0.83 1.56 0.98 1.22 0.82 0.23 0.57 0.72 0.29 1.6 1.23 0.89 2.56 1.37 0.93<br />
4860 RB5869_conserved hypothetical protein 3078569 3077796 -0.03 0.5 0.96 1.07 0.62 1.13 1.27 1.02 0.87 1.28 3.09 1.49 0.86 0.82 -0.08<br />
5158 RB6375_hypothetical protein 3349681 3350574 0.94 1.21 0.75 0.54 0.42 0.24 0.38 0.71 0.49 0.7 1.08 0.80 1.41 1.61 1.68<br />
6107 RB7901_hypothetical protein 4221613 4221293 1.06 0.43 1.13 0.75 0.3 1.24 1.92 1.34 1.66 2.41 1.26 NaN NaN 1.55 1.79<br />
6178 RB8029_oxidoreductase, short-chain dehydrogenase/reductase family 4292625 4291888 NaN 0.58 1.54 1 0.77 NaN 1.37 0.82 0.54 0.59 2.35 0.84 NaN 0.96 NaN<br />
6386 RB8385_hypothetical protein 4479935 4480087 NaN 0.72 0.92 1.6 0.38 NaN 1.46 1.02 0.82 0.81 0.85 NaN NaN 0.93 2.24<br />
7157 RB9699_Rhs element Vgr protein 5226252 5228345 NaN 0.57 1.05 0.97 0.81 NaN 0.57 NaN 1.02 1.63 0.60 NaN NaN 1.59 1.66<br />
264 RB10440_hypothetical protein 5655693 5655842 1.55 1.13 1.02 0.79 1.68 0.53 1.01 0.96 0.67 -0.17 1.42 1.48 1.23 1.81 1.31<br />
285 RB10473_conserved hypothetical protein 5679433 5681868 1.23 1 1.06 0.58 1.49 NaN 1.35 0.84 0.91 0.83 0.43 0.20 NaN 1.62 1.70<br />
658 RB11110_Rhodopirellula transposase 6022600 6023529 1.98 0.71 0.69 0.48 1.76 NaN 1.41 0.76 0.86 0.76 NaN NaN NaN 1.30 2.07<br />
719 RB11209_hypothetical protein 6080603 6080914 0.18 0.76 0.96 1.04 0.78 NaN 1.07 NaN 0.71 0.96 1.94 NaN NaN 1.86 1.71<br />
1058 RB11768_conserved hypothetical protein 6334808 6334939 0.4 0.2 0.97 0.7 0.6 NaN NaN 0.44 0.49 0.4 0.76 0.52 NaN 1.50 1.44<br />
2923 RB2875_hypothetical protein 1497313 1497534 0.04 0.62 1.03 1.12 0.44 0.53 0.62 0.54 0.16 0.44 2.42 1.73 1.62 1.43 0.82<br />
3238 RB3375_hypothetical protein 1740198 1740070 NaN 0.8 0.28 0.56 NaN NaN 1.22 NaN NaN 0.46 NaN NaN NaN NaN NaN<br />
3458 RB370_nitrate transporter substrate-binding protein 184428 186089 0.32 0.71 0.68 1.76 1.23 1.5 1.98 1.91 1.21 1.19 1.67 NaN NaN 2.39 2.96<br />
4858 RB5866_ISXo8 transposase 3076242 3077567 1.52 0.82 1.15 0.85 1.52 NaN 1.44 1.26 1.35 0.25 1.05 NaN NaN 1.98 NaN<br />
5373 RB671_hypothetical protein 359218 359586 1.8 1.02 0.61 0.89 2.03 NaN 1.24 0.76 0.78 1.02 0.91 0.51 2.60 1.43 0.92<br />
6416 RB8437_conserved hypothetical protein, secreted 4506842 4509907 NaN 0.29 1.05 1.52 0.55 NaN NaN NaN NaN NaN 2.69 NaN NaN NaN NaN<br />
181 RB10295_methylene tetrahydrofolate 5562427 5561501 1.23 0.85 1.45 0.92 0.95 NaN 1.52 0.92 1.24 1.37 1.02 NaN NaN 1.42 1.53<br />
491 RB10824_hypothetical protein 5878877 5878999 NaN 0.52 1.08 1.29 0.88 NaN 1.37 0.67 0.81 0.75 NaN NaN NaN NaN NaN<br />
1267 RB12132_conserved hypothetical protein 6548702 6548550 NaN NaN 0.48 NaN NaN NaN NaN NaN NaN 0.82 NaN NaN NaN NaN NaN<br />
1479 RB12460_protein containing DUF1571 6707170 6706103 NaN 0.69 1.04 0.8 0.91 NaN 1.25 NaN 0.87 0.71 NaN NaN NaN NaN NaN<br />
1643 RB12737_conserved hypothetical protein 6863917 6864114 1.08 0.99 1.45 1.3 1.87 0.35 0.92 1.03 0.81 0.14 1.26 1.19 2.09 2.02 1.98<br />
1897 RB13165_xylose operon regulatory protein 7067089 7068300 NaN 0.56 0.55 0.79 NaN NaN NaN NaN NaN 0.57 NaN NaN NaN NaN NaN<br />
1920 RB13202_conserved hypothetical protein 7086189 7086052 NaN 0.27 1.4 0.71 0.49 NaN NaN NaN NaN -0.15 NaN NaN NaN 1.44 NaN<br />
3126 RB3207_hypothetical protein 1652052 1652453 NaN NaN NaN 0.68 NaN NaN NaN NaN NaN 0.55 NaN NaN NaN NaN NaN<br />
4322 RB5_secreted protein 1514 399 NaN NaN 0.91 0.81 0.51 NaN -0.06 NaN -0.56 NaN NaN 0.01 NaN 3.14 2.35<br />
6538 RB8641_hypothetical protein 4605970 4606248 1.88 1.18 1.3 1.24 1.31 NaN NaN 0.25 NaN 0.63 1.52 0.36 0.56 2.61 2.42<br />
902 RB11505_conserved hypothetical protein, secreted 6221829 6221230 2.37 1.22 0.49 0.87 1.99 0.96 1.08 1.1 1.58 0.92 0.66 0.46 2.30 2.40 0.76<br />
1764 RB12932_conserved hypothetical protein 6963242 6963015 0.22 1 0.02 1.29 1.37 NaN 1.35 NaN NaN 0.72 NaN NaN NaN NaN NaN<br />
1946 RB13241_RNA polymerase ECF-type sigma factor 7103064 7102561 0.73 1.09 1.79 0.85 1.36 NaN 2.28 NaN 2.83 1.27 2.41 1.16 1.09 3.06 0.86<br />
3869 RB4340_Rhodopirellula transposase 2234846 2233917 0.62 0.83 0.56 1.13 1.59 NaN 1.15 NaN 0.66 0.31 NaN NaN NaN NaN NaN<br />
6399 RB8407_Endonuclease/exonuclease/phosphatase 4495545 4494700 NaN 0.41 NaN NaN NaN NaN NaN NaN NaN 0.71 NaN NaN NaN NaN NaN<br />
1332 RB12239_ISXo8 transposase 6593334 6594635 1.49 1.15 1.48 1.06 1.65 NaN 1.53 1.49 1.54 0.78 1.32 NaN NaN 2.57 NaN<br />
3921 RB4419_MgtC/SapB transporter 2272912 2273448 0.95 1.77 1.79 1.38 2.23 0.82 0.9 1.11 0.77 1.4 1.00 1.56 3.06 2.11 1.09<br />
2385 RB2019_hypothetical protein 1065619 1065798 NaN NaN NaN 0.59 NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN<br />
3201 RB3326_hypothetical protein 1711353 1711640 NaN 0.63 0.67 0.88 0.48 NaN NaN NaN NaN 1.09 NaN NaN NaN NaN NaN<br />
7307 RB9964_membrane protein 5368651 5367674 NaN NaN NaN NaN 0.81 NaN NaN NaN NaN 0.6 NaN NaN NaN NaN NaN<br />
1300 RB12194_SAICAR synthetase 6576972 6575974 NaN 0.84 0.77 NaN 0.48 NaN NaN NaN NaN 0.67 NaN NaN NaN NaN NaN<br />
2290 RB1854_protein containing DUF1080 958387 956276 NaN 0.46 0.97 NaN 0.45 NaN NaN NaN NaN 0.82 NaN NaN NaN NaN NaN<br />
5729 RB7284_hypothetical protein 3871505 3871639 NaN 0.22 0.83 1.11 0.19 NaN 0.81 0.6 NaN 0.68 NaN NaN NaN NaN NaN<br />
6021 RB7755_hypothetical protein 4154446 4154631 0.19 0.4 1.09 0.46 0.45 NaN 1 0.76 0.57 1.06 NaN NaN NaN NaN 0.84<br />
6372 RB8362_Serine/threonine protein phosphatase 4462144 4460687 0.24 0.74 0.34 NaN 0.68 NaN 1.14 0.69 0.43 0.82 NaN NaN NaN NaN NaN<br />
614 RB11030_ABC transporter, ATP-binding protein 5980829 5980074 NaN 0.49 1.32 0.58 0.72 NaN 0.83 NaN NaN 0.53 NaN NaN NaN NaN NaN<br />
4147 RB4748_hypothetical protein 2435328 2435465 0.37 NaN NaN NaN NaN 0.36 1.25 0.86 0.44 NaN NaN NaN NaN NaN NaN<br />
360 RB10607_hypothetical protein 5759926 5759537 1.78 0.21 0.38 0.15 0.99 NaN 0.17 NaN NaN 0.17 0.41 -0.04 1.74 1.36 -0.03<br />
1748 RB12895_conserved hypothetical protein 6945636 6946097 NaN 0.72 0.36 NaN 0.66 NaN NaN 0.8 NaN 0.25 NaN NaN NaN NaN NaN<br />
6652 RB8843_conserved hypothetical protein 4705018 4706850 NaN NaN NaN 0.8 NaN NaN NaN NaN NaN 0.43 NaN NaN NaN NaN NaN<br />
5930 RB7621_hypothetical protein 4097160 4098164 NaN 0.34 0.57 NaN 0.67 NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN<br />
2173 RB1632_hypothetical protein 839673 840332 NaN 0.39 0.22 0.75 NaN NaN NaN NaN NaN 0.48 NaN NaN NaN NaN NaN<br />
3060 RB3077_conserved hypothetical protein 1606106 1588083 NaN NaN NaN NaN NaN NaN NaN NaN NaN 0.64 NaN NaN NaN NaN NaN<br />
517 RB10875_hypothetical protein 5901192 5901043 NaN 0.51 -0.11 1.26 0.54 NaN 1.62 0.56 0.08 0.17 NaN NaN NaN NaN NaN<br />
6572 RB871_hypothetical protein 463841 462534 NaN 0.5 NaN NaN 0.72 NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN<br />
1304 RB1220_Aldose 1-epimerase 622840 623925 0.32 0.46 0.86 0.67 0.71 NaN 0.83 0.39 0.49 0.52 NaN NaN NaN NaN NaN<br />
5811 RB7440_hypothetical protein 3990113 3990535 NaN NaN NaN 0.58 NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
The Rhodopirellula baltica life cycle: Growth-dependent<br />
expression profiling of the complete genome with<br />
microarrays<br />
ADDITIONAL FILE 1 -<br />
List of differentially expressed genes<br />
ADDITIONAL FILE 4 -<br />
List of selected clusters out of the k-means clustering
62hvs44h_down<br />
ID Locus Ratio AA Nuc Product Gene EC Start Stop IEP MW(kDa) Strand<br />
547 RB1015 -1.62 222 669 membrane protein 531723 532391 5.9 24319 +<br />
5741 RB10299 -1.70 455 1368 conserverd hypothetical protein 5563036 5564403 10 49949 +<br />
5910 RB10623 -2.40 60 183 secreted protein 5769140 5768958 3.9 6284 -<br />
5921 RB10648 -1.61 310 933 conserved hypothetical protein 5779160 5778228 9.7 34602 -<br />
5938 RB10675 -1.82 779 2340 membrane protein 5802507 5800168 7.2 86215 -<br />
5939 RB10678 -1.73 116 351 hypothetical protein 5802466 5802816 12 13353 +<br />
63 RB110 -1.50 582 1749 choline-sulfatase 49093 47345 6 65751 -<br />
6308 RB11325 -1.92 47 144 conserved hypothetical protein 6142535 6142392 12.2 5354 -<br />
598 RB1137 -1.55 94 285 hypothetical protein 585204 585488 11.6 10805 +<br />
6818 RB12250 -1.53 104 315 conserved hypothetical protein 6597248 6597562 11.9 12364 +<br />
6870 RB12334 -1.59 266 801 Peptidase M50 3.4.24.- 6636156 6635356 5.4 29077 -<br />
6920 RB12426 -1.53 137 414 conserved hypothetical protein, membrane 6684059 6684472 10.6 15166 +<br />
6981 RB12526 -1.60 256 771 Rhomboid-like protein 6747132 6747902 6 27269 +<br />
7009 RB12581 -1.65 174 525 conserved hypothetical protein 6780597 6780073 9.9 19433 -<br />
7113 RB12770 -2.26 166 501 conserved hypothetical protein 6884806 6884306 11.9 18496 -<br />
691 RB1291 -1.74 97 294 hypothetical protein 656684 656977 10.4 10290 +<br />
7401 RB13324 -1.74 36 111 hypothetical protein 7141677 7141787 7.3 4100 +<br />
1016 RB1931 -1.96 68 207 hypothetical protein 1013831 1013625 11.4 7493 -<br />
1105 RB2105 -1.54 470 1413 membrane protein 1114012 1112600 9.6 51853 -<br />
1107 RB2110 -1.52 467 1404 multidrug resistance protein norM norM 1114250 1115653 9.6 50647 +<br />
1220 RB2306 -1.68 41 126 hypothetical protein 1206782 1206907 9 4759 +<br />
1265 RB2370 -1.59 54 165 hypothetical protein 1229187 1229023 6.3 6204 -<br />
1332 RB2479 -1.59 273 822 conserved hypothetical protein 1296683 1295862 5.5 30436 -<br />
1352 RB2503 -1.61 515 1548 O-antigen flippase wzx 1311056 1309509 10 55740 -<br />
1372 RB2529 -1.74 195 588 membrane protein 1320541 1321128 3.9 20867 +<br />
1407 RB2592 -1.57 197 594 hypothetical protein 1348926 1348333 4.7 22032 -<br />
1701 RB3147 -1.72 168 507 membrane protein 1624840 1624334 8.7 18759 -<br />
1742 RB3224 -1.58 148 447 hypothetical protein 1656004 1656450 12.4 16377 +<br />
1778 RB3281 -1.53 139 420 Histone-like bacterial DNA-binding protein 1687272 1686853 7.3 15802 -<br />
1806 RB3329 -1.66 64 195 hypothetical protein 1711937 1711743 12 6908 -<br />
1833 RB3372 -1.72 115 348 hypothetical protein 1733666 1734013 10 12916 +<br />
1857 RB3413 -1.66 373 1122 Esterase/lipase/ 1762402 1761281 5.1 40640 -<br />
1879 RB3450 -1.52 61 186 hypothetical protein 1782543 1782358 4.7 6530 -<br />
1984 RB3627 -1.65 280 843 Transglutaminase-like domain 1888482 1887640 4.5 30412 -<br />
2017 RB3675 -1.53 742 2229 secreted protein 1904552 1906780 8.4 82951 +<br />
22 RB37 -1.61 160 483 membrane protein 18328 17846 5.6 17283 -<br />
2154 RB3905 -1.89 38 117 hypothetical protein 2018601 2018717 5.1 4131 +<br />
2184 RB3953 -2.00 857 2574 hypothetical protein 2048784 2046211 5.2 93881 -<br />
2232 RB4032 -1.58 481 1446 secreted protein containing DUF1501 2084885 2083440 6.2 52620 -<br />
2260 RB4097 -1.88 733 2202 conserved hypothetical protein 2108244 2110445 6.2 83188 +<br />
2263 RB4104 -1.58 72 219 hypothetical protein 2112335 2112553 5.5 7870 +<br />
2298 RB4165 -1.57 721 2166 hypothetical protein 2139632 2141797 4.5 77994 +<br />
242 RB418 -1.96 363 1092 conserved hypothetical protein, membrane 222222 223313 10.3 39119 +<br />
2357 RB4266 -1.66 54 165 hypothetical protein 2183250 2183414 11.2 6166 +<br />
2359 RB4269 -1.61 282 849 glutamic acid specific endopeptidase, GSE=serine endopeptidase 2185049 2184201 5.6 31283 -<br />
2363 RB4273 -1.77 518 1557 iron-dependent peroxidase 1.1.1.- 2193431 2191875 5.1 57672 -<br />
250 RB434 -1.54 65 198 hypothetical protein 231791 231988 7.4 7385 +<br />
2437 RB4394 -1.70 188 567 protease I pfpI 3.2.-.- 2261372 2261938 4.4 20601 +<br />
2444 RB4402 -1.63 53 162 hypothetical protein 2265340 2265501 10.4 5980 +<br />
2451 RB4416 -1.85 833 2502 Cytochrome c oxidase, subunit I 2271803 2269302 5.8 92293 -<br />
2472 RB4454 -1.54 739 2220 Flagellin A flaA 2283201 2285420 4 76924 +<br />
2476 RB4458 -1.77 342 1029 Protein of unknown function, UPF0118 2287089 2286061 6.4 37001 -<br />
2477 RB4459 -1.52 462 1389 MscS Mechanosensitive ion channel 2288477 2287089 4.8 51157 -<br />
2512 RB4513 -1.54 326 981 serum resistance protein 2315098 2314118 7.4 35351 -<br />
2568 RB4613 -1.59 110 333 HesB/YadR/YfhF 2362510 2362178 4.6 12216 -<br />
277 RB472 -1.76 47 144 hypothetical protein 253266 253409 10.9 5373 +<br />
2669 RB4773 -1.87 208 627 hypothetical protein 2446723 2447349 10.8 23392 +<br />
2746 RB4897 -1.78 184 555 hypothetical protein 2519505 2518951 10.9 20446 -<br />
2763 RB4920 -2.34 69 210 hypothetical protein 2531850 2531641 4.4 7587 -<br />
2914 RB5174 -1.60 589 1770 Bacterial type II and III secretion system protein 2660936 2662705 4.8 63998 +<br />
3055 RB5424 -1.76 573 1722 sulfatase 2807339 2809060 5.7 64433 +<br />
3062 RB5434 -1.51 695 2088 Elongation factor G 1 fusA1 2817863 2815776 4.7 77788 -<br />
3146 RB5581 -1.68 397 1194 conserved hypothetical protein 2905973 2904780 4.8 44297 -<br />
3208 RB5695 -1.69 288 867 carbon-nitrogen hydrolase family 2971401 2972267 4.5 31927 +<br />
3212 RB5701 -1.50 1111 3336 oxidase subunit 2975188 2978523 9.1 122095 +<br />
3247 RB5761 -1.55 258 777 conserved hypothetical protein, membrane 3015202 3014426 10.9 27944 -<br />
3291 RB5841 -1.64 670 2013 serine/threonine protein phosphatase family 3061924 3063936 5.4 73664 +<br />
3307 RB5872 -1.51 83 252 hypothetical protein 3080059 3080310 13 9379 +<br />
3328 RB5903 -1.54 58 177 hypothetical protein 3094315 3094491 10.1 6651 +<br />
3389 RB6010 -1.65 53 162 hypothetical protein 3149911 3150072 11.9 5744 +<br />
3396 RB6019 -1.62 161 486 hypothetical protein 3154683 3154198 4.1 18098 -<br />
3400 RB6031 -1.55 492 1479 hypothetical protein 3160462 3158984 4.1 54352 -<br />
3407 RB6042 -1.55 415 1248 CBS domain protein 3166072 3164825 4.4 45534 -<br />
3527 RB6274 -1.71 59 180 hypothetical protein 3297130 3296951 12 7254 -<br />
3544 RB6305 -1.52 437 1314 dolichol-phosphate mannosyltransferase 3312842 3314155 8.9 47433 +<br />
3617 RB6423 -2.17 45 138 hypothetical protein 3380603 3380740 5.5 5402 +<br />
3625 RB6434 -1.77 48 147 hypothetical protein 3388682 3388536 7.4 5104 -<br />
3632 RB6443 -1.59 397 1194 Cys/Met metabolism pyridoxal-phosphate-dependent enzymes 3393276 3394469 5.3 42845 +<br />
3649 RB6470 -1.63 701 2106 Abortive infection protein 3420270 3422375 5.9 76321 +<br />
3667 RB6496 -1.57 57 174 hypothetical protein 3441948 3442121 9.9 6289 +<br />
3693 RB6537 -1.57 384 1155 Aldose 1-epimerase 5.1.3.3 3472890 3471736 4.7 42197 -<br />
3695 RB6539 -1.75 374 1125 membrane protein 3474598 3475722 10.1 41456 +<br />
3713 RB6574 -2.19 723 2172 protein containing DUF1585 3493615 3491444 5.2 81617 -<br />
3720 RB6587 -1.92 50 153 hypothetical protein 3501747 3501595 7.5 5579 -<br />
3732 RB6610 -1.68 1235 3708 conserved hypothetical protein 3511694 3515401 5.3 137776 +<br />
3738 RB6624 -1.63 503 1512 Bacterial sugar transferase 2.7.8.6 3520589 3522100 7.7 55315 +<br />
3742 RB6632 -1.68 55 168 hypothetical protein 3524963 3525130 5.6 6193 +<br />
3757 RB6663 -1.50 461 1386 Major facilitator superfamily MFS_1 3540255 3538870 9.9 50033 -<br />
3780 RB6699 -1.76 47 144 hypothetical protein 3559781 3559924 11.1 5290 +<br />
3795 RB6724 -1.54 131 396 hypothetical protein 3569189 3569584 10.5 14916 +<br />
3836 RB6789 -1.61 180 543 secreted protein 3608487 3607945 5 20191 -<br />
370 RB679 -1.54 60 183 hypothetical protein 362787 362605 10.7 7113 -<br />
3872 RB6858 -1.58 341 1026 dehydrogenases and related proteins 3646807 3645782 5.5 36680 -<br />
375 RB686 -1.66 549 1650 arylsulfatase atsA 3.1.6.1 370206 368557 5.4 62276 -<br />
3875 RB6864 -1.65 44 135 hypothetical protein 3648295 3648429 2.8 4573 +<br />
3923 RB6958 -1.55 188 567 conserved hypothetical protein 3695752 3695186 9.4 20788 -<br />
3971 RB7042 -1.63 91 276 hypothetical protein 3743971 3744246 10.4 10835 +<br />
3985 RB7072 -1.53 257 774 lipoate-protein ligase A lplA 6.3.2.- 3754556 3753783 5 29306 -<br />
4009 RB7109 -1.72 53 162 hypothetical protein 3775707 3775546 6.7 5850 -<br />
4092 RB7246 -1.50 50 153 hypothetical protein 3850393 3850545 9.7 5973 +<br />
4303 RB7628 -1.51 122 369 hypothetical protein 4104029 4103661 12.2 13804 -<br />
4578 RB8119 -1.60 142 429 hypothetical protein 4346427 4346855 10.1 15659 +<br />
4627 RB8223 -1.68 410 1233 protein containing DUF1559 4399759 4398527 6.3 44709 -<br />
5060 RB9034 -1.54 1031 3096 conserved hypothetical protein, secreted 4818355 4821450 5.4 113674 +<br />
5113 RB9141 -1.50 346 1041 rRNA (guanine-N(2)-)-methyltransferase 4891836 4892876 7.8 38276 +<br />
5156 RB9228 -1.51 637 1914 hypothetical protein 4934679 4936592 7.8 73174 +<br />
5240 RB9385 -1.56 507 1524 ribose transport ATP-binding protein rbsA rbsA 3.6.3.17 5033732 5032209 4.8 54554 -<br />
5275 RB9445 -1.78 249 750 integral membrane protein 5074872 5075621 10.4 26862 +<br />
5318 RB9529 -1.76 113 342 hypothetical protein 5117956 5118297 9.3 13082 +<br />
5386 RB9651 -2.07 544 1635 sialic acid-specific 9-O-acetylesterase 3.1.1.53 5198277 5196643 5.9 60120 -
62hvs44h_up<br />
ID Locus Ratio AA Nuc Product Gene EC Start Stop IEP MW(kDa) Strand<br />
5764 RB10341 2.27 61 186 hypothetical protein 5593416 5593601 4.5 6613 +<br />
5766 RB10343 3.62 53 162 hypothetical protein 5593898 5594059 11.3 6302 +<br />
5815 RB10440 3.33 49 150 hypothetical protein 5655693 5655842 11.5 5080 +<br />
6096 RB10951 3.24 101 306 secreted protein 5940232 5939927 10.2 11754 -<br />
584 RB1104 2.89 59 180 hypothetical protein 572078 572257 10.7 6510 +<br />
622 RB1179 1.84 128 387 hypothetical protein 603998 604384 12 13863 +<br />
6720 RB12068 1.63 553 1662 serine/threonine protein kinase 6512255 6510594 8.5 61271 -<br />
6785 RB12199 1.58 54 165 conserved hypothetical protein 6577614 6577778 11.3 5569 +<br />
6867 RB12329 2.02 110 333 conserved hypothetical protein, membrane 6634234 6634566 4 11862 +<br />
7023 RB12610 2.12 85 258 conserved hypothetical protein 6793700 6793957 9.7 9221 +<br />
7141 RB12818 1.59 163 492 conserved hypothetical protein 6915989 6915498 11.7 18010 -<br />
7151 RB12833 1.85 109 330 conserved hypothetical protein 6921305 6921634 12 12221 +<br />
7293 RB13115 2.31 81 246 conserved hypothetical protein 7036614 7036369 9.8 8735 -<br />
1028 RB1961 3.07 117 354 hypothetical protein 1033791 1034144 11.9 13392 +<br />
1330 RB2477 2.51 123 372 hypothetical protein 1295793 1295422 10.2 13737 -<br />
1706 RB3153 1.83 76 231 hypothetical protein (protein containing DUF1584) 1626911 1626681 12.4 8266 -<br />
1728 RB3196 1.65 109 330 hypothetical protein 1650093 1650422 10.1 12287 +<br />
1835 RB3374 1.50 95 288 hypothetical protein 1740350 1740063 11 11130 -<br />
195 RB341 1.67 90 273 hypothetical protein 169852 170124 11.2 10096 +<br />
1992 RB3644 2.98 127 384 hypothetical protein 1895184 1894801 4.9 14294 -<br />
2501 RB4498 1.51 90 273 hypothetical protein 2303522 2303250 9.4 9942 -<br />
2502 RB4499 2.06 81 246 hypothetical protein 2303764 2303519 10.7 9029 -<br />
2553 RB4590 1.51 74 225 hypothetical protein 2352024 2351800 10.1 8330 -<br />
3341 RB5929 1.86 74 225 hypothetical protein 3107256 3107480 12.1 8243 +<br />
3585 RB6375 1.86 297 894 hypothetical protein 3349681 3350574 8.7 31772 +<br />
3587 RB6378 2.75 228 687 hypothetical protein 3352598 3353284 12.3 25698 +<br />
3639 RB6454 1.67 93 282 hypothetical protein 3407776 3407495 11.7 10063 -<br />
3900 RB6910 3.07 42 129 hypothetical protein 3671484 3671356 9.5 4575 -<br />
3909 RB6925 3.02 161 486 hypothetical protein 3678461 3677976 9.8 17641 -<br />
3911 RB6930 2.24 413 1242 glutamate dehydrogenase 1.4.1.3 3680317 3679076 5.9 45734 -<br />
4422 RB7828 2.96 40 123 hypothetical protein 4192037 4191915 10.4 4526 -<br />
4590 RB8146 2.10 151 456 hypothetical protein 4355122 4354667 4.3 17016 -<br />
4609 RB8188 2.30 259 780 hydroxypyruvate isomerase hyi 5.3.1.22 4378573 4379352 4.4 28228 +<br />
4699 RB8357 1.72 56 171 hypothetical protein 4460174 4460004 7.4 6097 -<br />
4861 RB8669 2.61 37 114 hypothetical protein 4614557 4614670 11.5 4395 +<br />
4862 RB8670 2.47 47 144 hypothetical protein 4614842 4614985 11.1 5371 +<br />
4880 RB8700 1.51 58 177 hypothetical protein 4630224 4630400 4.8 6268 +<br />
4919 RB8775 2.49 81 246 hypothetical protein 4665497 4665252 5.3 8745 -<br />
5278 RB9450 3.13 52 159 hypothetical protein 5076581 5076739 7.3 5967 +
82 h vs 62 h down<br />
ID Locus RATIO AA Nuc Product EC Start Stop IEP MW(kDa) Strand<br />
6048 RB10868 -2.12 136 411 hypothetical protein 5900330 5899920 11.3 15100 -<br />
6097 RB10954 -1.83 143 432 hypothetical protein 5940749 5940318 10.4 16058 -<br />
6867 RB12329 -1.72 110 333 conserved hypothetical protein, membrane 6634234 6634566 4 11862 +<br />
7147 RB12827 -1.69 122 369 conserved hypothetical protein, membrane 6919729 6919361 12.5 13367 -<br />
7313 RB13148 -1.49 1012 3039 arylsulfatase A [precursor] 7058006 7054968 6.2 111097 -<br />
1008 RB1910 -2.39 123 372 hypothetical protein 993764 993393 11.2 13384 -<br />
1705 RB3152 -1.80 68 207 hypothetical protein 1626667 1626461 12.2 7261 -<br />
1827 RB3365 -1.85 43 132 hypothetical protein 1730124 1730255 11.4 4886 +<br />
1992 RB3644 -1.60 127 384 hypothetical protein 1895184 1894801 4.9 14294 -<br />
2093 RB3802 -1.86 64 195 hypothetical protein 1969456 1969262 9.7 6927 -<br />
2403 RB4338 -1.57 57 174 hypothetical protein 2233809 2233636 6.3 6607 -<br />
2493 RB4482 -1.67 111 336 hypothetical protein 2297240 2296905 12 12929 -<br />
2629 RB4715 -1.52 1291 3876 conserved hypothetical protein 2413294 2417169 5.3 138888 +<br />
2637 RB4727 -1.80 54 165 hypothetical protein 2422602 2422766 9.1 5908 +<br />
2698 RB4814 -1.51 87 264 hypothetical protein 2467537 2467800 11.3 9301 +<br />
2989 RB5303 -1.73 67 204 hypothetical protein 2736717 2736514 10.1 7354 -<br />
3049 RB5415 -1.58 62 189 hypothetical protein 2798737 2798925 12.3 7098 +<br />
3649 RB6470 -1.63 701 2106 Abortive infection protein 3420270 3422375 5.9 76321 +<br />
3909 RB6925 -1.91 161 486 hypothetical protein 3678461 3677976 9.8 17641 -<br />
3911 RB6930 -1.94 413 1242 glutamate dehydrogenase 1.4.1.3 3680317 3679076 5.9 45734 -<br />
4861 RB8669 -1.57 37 114 hypothetical protein 4614557 4614670 11.5 4395 +<br />
4880 RB8700 -2.34 58 177 hypothetical protein 4630224 4630400 4.8 6268 +<br />
5052 RB9020 -2.13 381 1146 secreted protein 4807486 4808631 5.9 42557 +<br />
5297 RB9493 -1.50 155 468 acetyltransferase 5096754 5097221 5.3 17410 +<br />
5545 RB9945 -1.86 317 954 secreted protein 5355360 5356313 5.1 35540 +
82 h vs 62 h up<br />
ID Locus Ratio AA Nuc Product Gene EC Start Stop IEP MW(kDa) Strand<br />
5649 RB10129 1.54 238 717 FKBP-type peptidyl-prolyl cis-trans isomerase fklB 5.2.1.8 5467429 5466713 4.9 26226 -<br />
5713 RB10242 2.44 330 993 Curved DNA-binding protein cbpA 5536118 5535126 6.7 35177 -<br />
5733 RB10279 2.63 151 456 heat shock protein, Hsp20 family 5557118 5557573 4.9 17072 +<br />
5734 RB10283 2.58 107 324 small heat shock protein 5557737 5558060 4.7 12267 +<br />
5741 RB10299 2.45 455 1368 conserverd hypothetical protein 5563036 5564403 10 49949 +<br />
5785 RB10384 2.44 96 291 hypothetical protein 5612174 5611884 4.1 10517 -<br />
5910 RB10623 2.03 60 183 secreted protein 5769140 5768958 3.9 6284 -<br />
5912 RB10629 1.76 539 1620 GroEL 5769500 5771119 4.9 57511 +<br />
5967 RB10727 2.64 276 831 manganese-containing catalase 5830775 5831605 5 30055 +<br />
5968 RB10728 1.70 132 399 secreted protein 5831649 5832047 9.9 13833 +<br />
6256 RB11242 2.49 201 606 RNA polymerase sigma-70 factor, ECF subfamily 6095778 6096383 7.6 22688 +<br />
6361 RB11421 1.73 552 1659 60-kDa SS-A/Ro ribonucleoprotein rsr 6195538 6193880 9.6 59999 -<br />
6414 RB11488 1.61 265 798 oxidoreductase, short-chain dehydrogenase/reductase family 6213893 6214690 5.1 27687 +<br />
625 RB1183 1.54 164 495 membrane protein 605059 605553 9.3 18224 +<br />
6611 RB11863 1.63 375 1128 RecA bacterial DNA recombination protein 6388538 6387411 5.5 39958 -<br />
6719 RB12067 1.86 107 324 conserved hypothetical protein 6510236 6510559 11.9 12169 +<br />
6832 RB12278 3.27 52 159 conserved hypothetical protein 6605390 6605548 11 5893 +<br />
6845 RB12296 1.66 339 1020 Short-chain dehydrogenase/reductase SDR 6615422 6614403 10.1 36620 -<br />
6903 RB12395 1.66 52 159 conserved hypothetical protein 6670730 6670572 10.1 5914 -<br />
6950 RB12471 1.82 85 258 conserved hypothetical protein 6712442 6712185 9.7 9841 -<br />
7078 RB12702 1.53 264 795 secreted protein 6844877 6844083 4.6 28179 -<br />
7277 RB13092 1.64 46 141 conserved hypothetical protein 7025003 7025143 5.4 4905 +<br />
7314 RB13150 1.83 527 1584 sialic acid-specific 9-O-acetylesterase 3.1.1.53 7059586 7058003 6.3 58659 -<br />
7340 RB13202 1.68 45 138 conserved hypothetical protein 7086189 7086052 9.4 4758 -<br />
804 RB1501 1.65 50 153 hypothetical protein 769419 769571 7.3 5710 +<br />
913 RB1722 2.37 651 1956 hypothetical protein 897688 899643 5.4 70377 +<br />
1566 RB2900 2.08 90 273 hypothetical protein 1506927 1507199 9.1 9939 +<br />
1606 RB2963 1.62 119 360 hypothetical protein 1526707 1527066 12.2 13440 +<br />
1740 RB3217 1.82 164 495 S-adenosylmethionine decarboxylase proenzyme speH 4.1.1.50 1654745 1654251 5.4 17585 -<br />
1868 RB3430 1.54 248 747 conserved hypothetical protein, membrane 1772646 1773392 7.5 26767 +<br />
1985 RB3630 2.06 313 942 conserved hypothetical protein 1888468 1889409 6.3 34702 +<br />
2065 RB3751 1.95 371 1116 NAD-dependent epimerase/dehydratase 1938953 1940068 5.5 40775 +<br />
2288 RB4147 1.54 136 411 hypothetical protein 2131928 2132338 5.5 15439 +<br />
2304 RB4176 1.90 96 291 hypothetical protein 2146928 2146638 12.4 11402 -<br />
2389 RB4308 2.04 79 240 protein containing DUF1586 2221461 2221222 11.9 9086 -<br />
2568 RB4613 2.48 110 333 HesB/YadR/YfhF 2362510 2362178 4.6 12216 -<br />
2607 RB4676 1.75 221 666 hypothetical protein 2391079 2390414 10.4 23751 -<br />
2608 RB4678 1.53 199 600 secreted protein 2390618 2391217 4.2 20927 +<br />
2649 RB4742 1.60 324 975 Universal stress protein uspE 2433589 2432615 4.7 35517 -<br />
2693 RB4809 2.08 98 297 membrane protein 2464977 2465273 10.1 10285 +<br />
2696 RB4812 1.88 92 279 secreted protein 2467011 2467289 12.5 10014 +<br />
2726 RB4867 1.80 210 633 SmpB RNA-binding protein SmpB 2496354 2495722 10.8 23762 -<br />
2740 RB4886 1.56 67 204 hypothetical protein 2513213 2513010 12.4 7587 -<br />
2750 RB4903 1.91 793 2382 xylulose-5-phosphate/fructose-6-phosphate phosphoketolase xfp 4.1.2.9 2520925 2523306 6 89499 +<br />
4 RB5 1.71 371 1116 secreted protein 1514 399 4.1 41410 -<br />
2834 RB5036 1.54 56 171 hypothetical protein 2590789 2590959 9.3 5985 +<br />
2951 RB5238 1.79 73 222 hypothetical protein 2697775 2697996 10.6 7866 +<br />
328 RB568 3.58 77 234 hypothetical protein 312345 312112 10.2 8689 -<br />
3294 RB5848 1.99 140 423 dnaK suppressor protein, 3066328 3065906 4.6 15633 -<br />
3340 RB5927 1.50 445 1338 protein containing DUF1501 3107242 3105905 6.4 48462 -<br />
3615 RB6417 2.23 249 750 conserved hypothetical protein 3378045 3378794 5.9 27679 +<br />
3724 RB6595 1.90 234 705 Rickettsia 17 kDa surface antigen 3506173 3505469 10.1 24227 -<br />
3812 RB6751 1.78 859 2580 chaperone ClpB clpB 3586111 3588690 5.2 95764 +<br />
3866 RB6848 1.86 75 228 secreted protein 3642216 3642443 3.8 8093 +<br />
3993 RB7085 2.58 75 228 hypothetical protein 3763662 3763435 10.4 8270 -<br />
4142 RB7362 1.77 85 258 hypothetical protein 3939758 3939501 5.6 9005 -<br />
4197 RB7452 1.78 170 513 conserved hypothetical protein 3994713 3995225 5.7 19622 +<br />
4291 RB7612 1.55 136 411 hypothetical protein 4092907 4093317 11.4 15607 +<br />
4292 RB7613 1.71 46 141 hypothetical protein 4093558 4093418 12.4 5359 -<br />
4735 RB8421 1.55 156 471 conserved hypothetical protein, membrane 4501840 4502310 10.3 16657 +<br />
5026 RB8966 2.04 660 1983 Chaperonin Cpn60/TCP-1 4777493 4779475 5.3 69819 +<br />
5094 RB9105 2.31 704 2115 Heat shock protein Hsp70 dnaK 4868834 4866720 4.6 76038 -<br />
5234 RB9372 2.43 205 618 Imidazole glycerol phosphate synthase subunit hisH hisH 2.4.2.- 5021363 5020746 4.9 22047 -<br />
5377 RB9635 1.51 352 1059 hypothetical protein 5187031 5185973 5.9 40195 -<br />
5571 RB9999 2.22 281 846 conserved hypothetical protein-putative transcriptional regulator 5386828 5385983 4.6 29948 -
92h vs 82h down<br />
ID<br />
Locus<br />
Ratio<br />
AA<br />
Nuc<br />
Product<br />
EC<br />
Start<br />
Stop<br />
IEP<br />
MW(kDa)<br />
5688 RB10199 -2.24 45 138 hypothetical protein 5512721 5512584 11.1 5010 -<br />
5741 RB10299 -1.73 455 1368 conserverd hypothetical protein 5563036 5564403 10 49949 +<br />
5933 RB10668 -1.95 58 177 hypothetical protein 5796528 5796352 9.3 6645 -<br />
5973 RB10734 -1.60 277 834 conserved hypothetical protein, secreted 5833947 5834780 3.9 29392 +<br />
6088 RB10937 -1.48 61 186 hypothetical protein 5934561 5934376 9.7 6825 -<br />
6222 RB11184 -1.51 41 126 secreted protein 6068441 6068316 10.6 4542 -<br />
6640 RB11922 -1.53 150 453 membrane protein containing DUF420 6419037 6418585 10.2 17134 -<br />
6711 RB12053 -1.50 119 360 biopolymer transport protein, ExbD/TolR family 6502558 6502199 4.2 12926 -<br />
655 RB1230 -2.95 117 354 hypothetical protein 627328 626975 11.7 13093 -<br />
6867 RB12329 -2.44 110 333 conserved hypothetical protein, membrane 6634234 6634566 4 11862 +<br />
663 RB1240 -2.43 504 1515 protein containing DUF1080 634060 632546 9.4 55473 -<br />
678 RB1269 -3.09 203 612 hypothetical protein 649412 648801 11.9 22612 -<br />
7071 RB12691 -1.76 59 180 conserved hypothetical protein 6834264 6834085 10.7 6778 -<br />
7313 RB13148 -2.64 1012 3039 arylsulfatase A [precursor] 7058006 7054968 6.2 111097 -<br />
748 RB1395 -2.33 319 960 secreted protein similar to DNA-binding protein 712097 713056 5.5 35212 +<br />
811 RB1513 -1.59 98 297 secreted protein 775203 774907 11.6 10491 -<br />
928 RB1751 -2.24 68 207 hypothetical protein 910027 909821 10.5 7181 -<br />
957 RB1814 -1.72 244 735 hypothetical protein 939221 938487 12 26734 -<br />
1098 RB2096 -1.49 327 984 hypothetical protein 1106467 1105484 5.1 36441 -<br />
1472 RB2714 -1.59 302 909 protein containing DUF541 1415817 1414909 9.5 33087 -<br />
1500 RB2764 -1.68 841 2526 secreted protein containing DUF1585 1442155 1439630 5 94741 -<br />
1603 RB2958 -1.48 79 240 hypothetical protein 1524723 1524962 11.8 8676 +<br />
1673 RB3074 -1.99 53 162 hypothetical protein 1585325 1585164 11.2 5818 -<br />
1705 RB3152 -1.65 68 207 hypothetical protein 1626667 1626461 12.2 7261 -<br />
1835 RB3374 -1.75 95 288 hypothetical protein 1740350 1740063 11 11130 -<br />
1992 RB3644 -2.27 127 384 hypothetical protein 1895184 1894801 4.9 14294 -<br />
2057 RB3739 -1.64 139 420 conserved hypothetical protein 1935471 1935052 4 15381 -<br />
2093 RB3802 -2.09 64 195 hypothetical protein 1969456 1969262 9.7 6927 -<br />
2139 RB3880 -1.64 82 249 hypothetical protein 2006953 2006705 10.7 9535 -<br />
2166 RB3925 -1.57 50 153 hypothetical protein 2033534 2033382 10.2 5399 -<br />
2181 RB3949 -1.98 79 240 hypothetical protein 2042289 2042528 6.7 8631 +<br />
2198 RB3975 -1.54 204 615 hypothetical protein 2056726 2057340 12.3 22602 +<br />
2200 RB3981 -1.86 161 486 hypothetical protein 2058000 2058485 4.1 18283 +<br />
2359 RB4269 -1.57 282 849 glutamic acid specific endopeptidase, GSE=serine endopeptidase 2185049 2184201 5.6 31283 -<br />
2436 RB4393 -1.51 73 222 hypothetical protein 2261154 2261375 11.9 8421 +<br />
2593 RB4652 -1.59 52 159 hypothetical protein 2380494 2380652 12.6 5628 +<br />
2596 RB4657 -1.84 123 372 hypothetical protein 2381865 2382236 12.2 13398 +<br />
277 RB472 -1.58 47 144 hypothetical protein 253266 253409 10.9 5373 +<br />
2637 RB4727 -2.98 54 165 hypothetical protein 2422602 2422766 9.1 5908 +<br />
2654 RB4750 -2.71 204 615 membrane protein 2435462 2436076 10 23312 +<br />
2669 RB4773 -1.50 208 627 hypothetical protein 2446723 2447349 10.8 23392 +<br />
2740 RB4886 -1.74 67 204 hypothetical protein 2513213 2513010 12.4 7587 -<br />
2970 RB5271 -1.78 89 270 hypothetical protein 2717327 2717596 10.4 9809 +<br />
2989 RB5303 -2.50 67 204 hypothetical protein 2736717 2736514 10.1 7354 -<br />
3009 RB5347 -2.27 50 153 hypothetical protein 2764290 2764442 11.9 5353 +<br />
3049 RB5415 -1.52 62 189 hypothetical protein 2798737 2798925 12.3 7098 +<br />
3132 RB5555 -1.78 54 165 hypothetical protein 2891274 2891438 6.2 5971 +<br />
3149 RB5587 -1.52 219 660 secreted protein 2908004 2907345 8.9 23917 -<br />
35 RB59 -1.50 89 270 hypothetical protein 25208 24939 12.3 9957 -<br />
3466 RB6158 -1.51 142 429 hypothetical protein 3233532 3233104 6 15746 -<br />
3485 RB6193 -1.86 62 189 hypothetical protein 3246096 3245908 11.4 6970 -<br />
3547 RB6314 -1.88 52 159 hypothetical protein 3319320 3319478 6.3 6180 +<br />
3718 RB6583 -1.62 996 2991 secreted protein 3498012 3501002 4.5 112232 +<br />
3725 RB6594 -1.82 295 888 hypothetical protein 3505472 3506359 10.2 32951 +<br />
3806 RB6740 -1.58 330 993 nonspecific acid phosphatase precursor 3579474 3580466 5.3 36852 +<br />
3836 RB6789 -1.53 180 543 secreted protein 3608487 3607945 5 20191 -<br />
4066 RB7207 -1.61 119 360 hypothetical protein 3822537 3822896 10.5 13512 +<br />
4219 RB7494 -1.61 345 1038 oxidoreductase protein 4019046 4020083 5.7 38758 +<br />
4241 RB7535 -1.94 136 411 hypothetical protein 4050024 4050434 10.9 15058 +<br />
4657 RB8279 -1.49 71 216 hypothetical protein 4425128 4425343 5.8 7835 +<br />
4990 RB8895 -2.02 418 1257 Sialidase 3.2.1.18 4736087 4737343 6.3 45859 +<br />
5052 RB9020 -2.63 381 1146 secreted protein 4807486 4808631 5.9 42557 +<br />
5109 RB9132 -1.68 425 1278 conserved hypothetical protein 4886727 4888004 5.5 46519 +<br />
5217 RB9346 -1.86 71 216 hypothetical protein 5008335 5008550 10.5 7755 +<br />
5347 RB9578 -1.50 66 201 hypothetical protein 5150392 5150592 5.4 6959 +<br />
5455 RB9786 -1.68 146 441 hypothetical protein 5270645 5270205 12 16326 -<br />
5545 RB9945 -3.42 317 954 secreted protein 5355360 5356313 5.1 35540 +<br />
Strand
92h vs 82h up<br />
ID Locus Ratio Parent ID AA Nuc Product Gene EC Start Stop IEP MW(kDa) Strand<br />
5576 RB10007 2.48 1 313 942 conserved hypothetical protein 5390130 5391071 5 34128 +<br />
5647 RB10126 2.24 1 43 132 hypothetical protein 5465210 5465341 12.9 4964 +<br />
5694 RB10209 2.01 1 110 333 H+-transporting two-sector ATPase, C subunit 3.6.3.14 5515135 5515467 10.2 11565 +<br />
5722 RB10261 3.21 1 81 246 Carbon storage regulator csrA 5545964 5545719 5.1 8704 -<br />
5723 RB10263 2.07 1 58 177 hypothetical protein 5546338 5546162 9.6 6643 -<br />
5754 RB10326 1.84 1 588 1767 sulfate permease family protein 5580833 5582599 6 62785 +<br />
5765 RB10342 2.17 1 102 309 hypothetical protein 5593929 5593621 12.2 11300 -<br />
5823 RB10458 1.54 1 206 621 Rnpc2-prov protein 5671130 5670510 10.3 21043 -<br />
5838 RB10483 1.84 1 133 402 conserved hypothetical protein 5685437 5685838 5.7 14433 +<br />
5911 RB10627 2.48 1 93 282 Chaperonin Cpn10 5769148 5769429 3.9 9966 +<br />
5967 RB10727 3.91 1 276 831 manganese-containing catalase 5830775 5831605 5 30055 +<br />
6043 RB10861 3.60 1 46 141 hypothetical protein 5896851 5896991 4.2 4833 +<br />
6047 RB10867 1.71 1 207 624 hypothetical protein 5899359 5899982 5.7 23041 +<br />
6085 RB10932 3.11 1 65 198 secreted protein 5933872 5934069 3.8 6983 +<br />
6089 RB10939 1.69 1 62 189 conserved hypothetical protein 5934628 5934816 4.3 6627 +<br />
6096 RB10951 1.51 1 101 306 secreted protein 5940232 5939927 10.2 11754 -<br />
6100 RB10958 3.41 1 158 477 hypothetical protein 5941015 5941491 5.4 18101 +<br />
6103 RB10966 2.02 1 60 183 Protein of unknown function, UPF0057 5942934 5942752 10.1 6793 -<br />
6104 RB10967 2.40 1 341 1026 quinone oxidoreductase-like protein 5944040 5943015 4.5 37393 -<br />
6106 RB10971 2.81 1 303 912 Short-chain dehydrogenase/reductase SDR 5946406 5945495 9.8 32799 -<br />
6127 RB11008 3.55 1 394 1185 secreted protein 5967078 5968262 5.1 42891 +<br />
6133 RB11016 1.59 1 369 1110 protein containing DUF1559 5974211 5973102 5.4 39469 -<br />
6135 RB11021 1.60 1 295 888 ATP-binding protein of ABC transporter 5975385 5974498 5 31784 -<br />
6140 RB11031 1.66 1 62 189 hypothetical protein 5980865 5981053 12.1 7649 +<br />
6144 RB11041 1.79 1 349 1050 secreted protein containing DUF1559 5987234 5988283 6.1 38326 +<br />
6157 RB11070 1.71 1 52 159 hypothetical protein 6000321 6000479 10.2 5582 +<br />
6201 RB11150 1.94 1 385 1158 NADH:flavin oxidoreductase/NADH oxidase 6046862 6048019 4.5 42128 +<br />
6216 RB11174 1.63 1 469 1410 metallo-beta-lactamase family protein 6065046 6063637 4.8 51160 -<br />
6265 RB11258 2.39 1 237 714 conserved hypothetical protein, membrane 6106840 6106127 10.3 26175 -<br />
6268 RB11266 1.84 1 304 915 3',5'-cyclic-nucleotide phosphodiesterase 6108475 6109389 9.7 33880 +<br />
6269 RB11268 1.83 1 97 294 hypothetical protein 6109768 6109475 10.8 11235 -<br />
6346 RB11396 1.50 1 36 111 conserved hypothetical protein 6178445 6178555 11.9 4104 +<br />
6347 RB11397 3.07 1 207 624 SOUL heme-binding protein 6178540 6179163 8.7 23072 +<br />
6348 RB11399 2.49 1 132 399 conserved hypothetical protein 6179167 6179565 6.5 15047 +<br />
6362 RB11424 4.52 1 158 477 conserved hypothetical protein 6195656 6196132 11.1 17372 +<br />
6363 RB11427 2.45 1 93 282 conserved hypothetical protein 6196136 6196417 9.7 10315 +<br />
6364 RB11429 4.88 1 37 114 conserved hypothetical protein 6196478 6196591 12.4 4245 +<br />
6367 RB11434 2.50 1 69 210 conserved hypothetical protein 6197442 6197233 9.8 7670 -<br />
6371 RB11439 4.95 1 93 282 conserved hypothetical protein 6197993 6198274 10.6 10906 +<br />
6382 RB11446 2.40 1 113 342 conserved hypothetical protein 6199492 6199833 10 12579 +<br />
6385 RB11450 3.94 1 60 183 conserved hypothetical protein 6200046 6200228 11.8 6517 +<br />
6414 RB11488 1.74 1 265 798 oxidoreductase, short-chain dehydrogenase/reductase family 6213893 6214690 5.1 27687 +<br />
6417 RB11492 1.64 1 95 288 conserved hypothetical protein, membrane 6216103 6216390 12.3 10176 +<br />
6425 RB11505 1.68 1 199 600 conserved hypothetical protein, secreted 6221829 6221230 7.5 21660 -<br />
6718 RB12066 2.33 1 135 408 hypothetical protein 6510147 6510554 10 14924 +<br />
6766 RB12160 2.53 1 108 327 thioredoxin trxA 6563104 6562778 4 11608 -<br />
6836 RB12283 2.92 1 52 159 conserved hypothetical protein 6609220 6609378 7.6 5927 +<br />
6837 RB12285 2.19 1 224 675 phospholipid N-methyltransferase 2.1.1.17 6609391 6610065 6.8 24262 +<br />
6846 RB12297 2.40 1 169 510 conserved hypothetical protein 6615598 6616107 4.4 17963 +<br />
6884 RB12362 2.03 1 274 825 bacterioferritin comigratory protein 6653786 6652962 5.8 30428 -<br />
6890 RB12377 1.49 1 284 855 Ribosomal RNA adenine methylase transferase 6661963 6661109 5.7 31560 -<br />
673 RB1259 1.79 1 59 180 hypothetical protein 642452 642631 10.5 6484 +<br />
7069 RB12689 2.18 1 106 321 secreted protein 6833004 6833324 9.8 11239 +<br />
7149 RB12830 1.83 1 102 309 conserved hypothetical protein 6920508 6920200 10.9 11753 -<br />
7153 RB12837 1.92 1 65 198 conserved hypothetical protein 6921732 6921929 9.8 7318 +<br />
7295 RB13117 1.68 1 156 471 nitrogen regulation protein 7036595 7037065 4.6 17819 +<br />
7341 RB13205 2.60 1 75 228 hypothetical protein 7086564 7086337 12.4 8283 -<br />
7342 RB13207 3.98 1 58 177 secreted protein 7086761 7086585 11.9 6443 -<br />
707 RB1322 2.28 1 35 108 hypothetical protein 671526 671633 12.8 3914 +<br />
7369 RB13257 2.81 1 286 861 2-hydroxy-6-ketonona-2,4-dienedioic acid hydrolase bphD 3.7.1.- 7105590 7106450 4.6 32540 +<br />
733 RB1362 1.85 1 82 249 hypothetical protein 696352 696600 10.4 9054 +<br />
804 RB1501 2.68 1 50 153 hypothetical protein 769419 769571 7.3 5710 +<br />
839 RB1563 2.09 1 136 411 hypothetical protein 798934 799344 10.2 14882 +<br />
883 RB1644 3.27 1 123 372 secreted protein 845824 845453 9.7 13720 -<br />
915 RB1728 2.29 1 285 858 membrane protein containing DUF1206 899954 900811 9.9 30055 +<br />
946 RB1787 1.88 1 72 219 hypothetical protein 925904 925686 9.6 8167 -<br />
1053 RB2011 2.47 1 93 282 hypothetical protein 1059411 1059692 4.4 9880 +<br />
1182 RB2244 1.85 1 192 579 Glutathione peroxidase GPXMC1 1.11.1.12 1178675 1179253 5.2 20717 +<br />
1183 RB2247 3.12 1 579 1740 conserved hypothetical protein containing YTV domains 1181238 1179499 9.8 65013 -<br />
1275 RB2382 1.61 1 75 228 hypothetical protein 1238921 1239148 9.7 8092 +<br />
1278 RB2386 1.98 1 528 1587 RND efflux membrane fusion protein 1244320 1242734 6 58921 -<br />
1280 RB2394 3.13 1 236 711 conserved hypothetical protein 1245630 1244920 7 27126 -<br />
1341 RB2492 2.12 1 69 210 hypothetical protein 1302190 1302399 9.9 7373 +<br />
1364 RB2516 1.76 1 63 192 hypothetical protein 1316669 1316860 11.2 6506 +<br />
1428 RB2636 2.94 1 116 351 hypothetical protein 1368450 1368100 11.7 12761 -<br />
1452 RB2679 1.53 1 173 522 hypothetical protein 1391557 1392078 5.5 19502 +<br />
1453 RB2680 2.91 1 467 1404 conserved hypothetical protein, membrane 1393566 1392163 6.4 49834 -<br />
1505 RB2782 3.32 1 589 1770 protein containing DUF1559 1445576 1447345 7.2 63826 +<br />
1566 RB2900 1.94 1 90 273 hypothetical protein 1506927 1507199 9.1 9939 +<br />
1567 RB2901 2.39 1 392 1179 conserved hypothetical protein, secreted 1507210 1508388 4.8 42805 +<br />
177 RB309 1.94 1 482 1449 Magnesium chelatase, ChlI subunit chlI 6.6.1.1 156441 154993 5.1 53979 -<br />
1786 RB3295 2.35 1 140 423 secreted protein 1692681 1692259 3.9 14786 -<br />
1787 RB3298 2.24 1 39 120 hypothetical protein 1692797 1692678 12.2 4684 -<br />
1882 RB3455 2.38 1 57 174 hypothetical protein 1785458 1785631 10.2 6258 +<br />
2024 RB3686 2.12 1 327 984 oxidoreductase, zinc-binding 1910523 1909540 5.5 35045 -<br />
2059 RB3742 2.18 1 718 2157 Flagellin, N-terminal 1935693 1937849 3.8 72031 +<br />
2239 RB4051 1.68 1 180 543 hypothetical protein 2092752 2092210 10.1 20712 -<br />
2243 RB4060 2.42 1 190 573 TadE-like 2095403 2094831 10.2 20868 -<br />
2308 RB4185 1.51 1 89 270 hypothetical protein 2151951 2152220 12 9730 +<br />
2338 RB4230 2.31 1 331 996 conserved hypothetical protein 2168783 2167788 5.3 37197 -<br />
2351 RB4256 3.13 1 358 1077 conserved hypothetical protein, secreted 2180303 2181379 4.5 37091 +<br />
2401 RB4336 2.54 1 372 1119 conserved hypothetical protein 2231697 2232815 5.6 41876 +<br />
2429 RB4381 2.50 1 59 180 hypothetical protein 2255621 2255442 10.7 7087 -<br />
2430 RB4382 1.53 1 555 1668 Diaminopimelate decarboxylase lysA 4.1.1.20 2257285 2255618 7.7 62732 -<br />
2437 RB4394 1.61 1 188 567 protease I pfpI 3.2.-.- 2261372 2261938 4.4 20601 +<br />
2440 RB4397 2.12 1 55 168 protein containing DUF1560 2263976 2264143 10.5 5816 +<br />
2442 RB4399 2.02 1 328 987 zinc-binding oxidoreductase 2265237 2264251 4.6 35121 -<br />
2443 RB4400 2.51 1 58 177 hypothetical protein 2265382 2265206 9 6610 -<br />
2445 RB4404 1.82 1 302 909 oxidoreductase, short chain dehydrogenase/reductase family 2265539 2266447 4.9 32804 +<br />
2453 RB4419 2.71 1 178 537 MgtC/SapB transporter 2272912 2273448 6.2 18995 +<br />
2456 RB4423 1.72 1 165 498 cytidine and deoxycytidylate deaminase family protein 2275085 2274588 4.7 17104 -<br />
2462 RB4432 2.69 1 268 807 glucose 1-dehydrogenase or 3-oxoacyl-[acyl-carrier-protein] reductase 1.1.1.- 2276333 2277139 4.7 28181 +<br />
2464 RB4436 1.84 1 90 273 membrane protein 2277820 2278092 12.5 9479 +<br />
2478 RB4460 1.51 1 422 1269 conserved hypothetical protein, membrane 2289696 2288428 4.8 48179 -<br />
2481 RB4464 1.87 1 223 672 secreted protein 2292075 2291404 5.1 25669 -<br />
2485 RB4468 2.36 1 162 489 hypothetical protein 2294104 2293616 4.6 16380 -<br />
2486 RB4470 2.85 1 169 510 secreted protein 2294676 2294167 4.9 19277 -<br />
2488 RB4474 3.76 1 168 507 CsbD-like 2295048 2295554 8.8 18451 +<br />
2489 RB4476 3.10 1 128 387 hypothetical protein 2295557 2295943 11.4 14037 +<br />
2490 RB4477 2.70 1 69 210 hypothetical protein 2295940 2296149 10.6 7470 +<br />
2491 RB4478 2.30 1 172 519 membrane protein 2296122 2296640 7.3 18536 +<br />
2492 RB4480 1.85 1 89 270 hypothetical protein 2296646 2296915 6.1 9442 +<br />
2494 RB4484 2.16 1 432 1299 Peptidase M20 3.-.-.- 2297267 2298565 6 46450 +<br />
2496 RB4487 1.93 1 514 1545 two-component system response regulator 2299859 2301403 4.6 56207 +
2498 RB4492 2.31 1 101 306 hypothetical protein 2302138 2301833 9.9 10841 -<br />
2500 RB4497 3.12 1 49 150 hypothetical protein 2302950 2303099 10.3 5351 +<br />
2504 RB4502 1.53 1 59 180 Protein of unknown function, UPF0057 2303992 2304171 9.1 6443 +<br />
2509 RB4510 1.60 1 49 150 hypothetical protein 2307657 2307508 9 5554 -<br />
2816 RB5001 3.33 1 103 312 hypothetical protein 2572354 2572665 10.3 11694 +<br />
2817 RB5003 3.11 1 498 1497 Rh-like protein/ammonium transporter AMT1.4 2572662 2574158 4 51180 +<br />
296 RB514 1.54 1 56 171 hypothetical protein 284567 284397 10.2 6284 -<br />
2908 RB5164 3.00 1 218 657 hypothetical protein 2658365 2657709 11.4 24275 -<br />
2913 RB5173 2.75 1 44 135 hypothetical protein 2660933 2660799 12.2 4858 -<br />
2927 RB5196 2.39 1 701 2106 trehalose synthase treS 5.4.99.16 2678058 2675953 5.6 81037 -<br />
301 RB521 2.57 1 63 192 hypothetical protein 286399 286208 10.7 7040 -<br />
2967 RB5266 1.60 1 45 138 hypothetical protein 2715721 2715584 11.8 5097 -<br />
3047 RB5412 1.69 1 72 219 hypothetical protein 2794838 2794620 12.3 8592 -<br />
317 RB548 1.51 1 633 1902 1,4-alpha-glucan branching enzyme glgB 2.4.1.18 306230 304329 4.4 72000 -<br />
3215 RB5707 2.80 1 183 552 hypothetical protein 2979435 2979986 10.2 20474 +<br />
3233 RB5737 1.56 1 120 363 hypothetical protein 2994415 2994777 11.9 13608 +<br />
3295 RB5850 2.37 1 63 192 hypothetical protein 3066498 3066307 5.8 7096 -<br />
3334 RB5914 3.77 1 56 171 hypothetical protein 3097264 3097434 10.5 6574 +<br />
3478 RB6178 2.15 1 41 126 hypothetical protein 3240162 3240287 12.7 4414 +<br />
3528 RB6276 1.82 1 105 318 Histone-like bacterial DNA-binding protein 3297504 3297187 10.4 11299 -<br />
3588 RB6379 3.01 1 153 462 hypothetical protein 3353122 3352661 5.8 17398 -<br />
3620 RB6428 3.39 1 164 495 conserved hypothetical protein, secreted 3382816 3382322 6.5 17292 -<br />
3621 RB6429 3.74 1 61 186 hypothetical protein 3382998 3382813 12.1 7095 -<br />
362 RB663 2.72 1 79 240 hypothetical protein 357608 357369 11.4 8792 -<br />
3770 RB6684 2.45 1 105 318 hypothetical protein 3554034 3553717 11.6 11612 -<br />
3826 RB6777 3.04 1 137 414 hypothetical protein 3602786 3603199 11.5 15512 +<br />
3901 RB6911 2.09 1 430 1293 protein containing DUF1559 3671445 3672737 6 45752 +<br />
3902 RB6912 3.15 1 55 168 secreted protein 3672793 3672960 9 5999 +<br />
3909 RB6925 2.05 1 161 486 hypothetical protein 3678461 3677976 9.8 17641 -<br />
4049 RB7178 1.70 1 45 138 hypothetical protein 3806924 3807061 12.3 5320 +<br />
392 RB723 1.78 1 60 183 hypothetical protein 388051 388233 7.3 6624 +<br />
4131 RB7313 2.26 1 51 156 hypothetical protein 3892097 3891942 10.5 5558 -<br />
4203 RB7464 2.38 1 44 135 hypothetical protein 4001614 4001480 10.2 4711 -<br />
4315 RB7647 4.19 1 73 222 hypothetical protein 4111931 4112152 7.4 8269 +<br />
4327 RB7667 1.75 1 38 117 hypothetical protein 4117097 4116981 6.7 4315 -<br />
4425 RB7833 2.41 1 228 687 Ribosomal protein L3 4192692 4193378 11 24681 +<br />
4518 RB8011 2.06 1 394 1185 secreted protein containing DUF1571 4282298 4281114 5.2 43074 -<br />
4588 RB8143 1.79 1 72 219 hypothetical protein 4354179 4353961 10.3 8288 -<br />
4589 RB8144 3.31 1 143 432 hypothetical protein 4354622 4354191 10.1 16269 -<br />
4696 RB8353 2.43 1 82 249 hypothetical protein 4459374 4459622 10.8 9402 +<br />
457 RB839 2.22 1 60 183 hypothetical protein 441662 441844 6.1 6654 +<br />
4749 RB8457 2.14 1 113 342 hypothetical protein 4518458 4518117 11.5 12525 -<br />
4791 RB8527 2.90 1 330 993 protein containing DUF1559 4549097 4550089 6.6 35645 +<br />
4940 RB8814 2.75 1 78 237 hypothetical protein 4687433 4687669 10.1 8719 +<br />
4941 RB8815 2.55 1 71 216 hypothetical protein 4687955 4687740 4.8 7683 -<br />
5023 RB8959 1.77 1 662 1989 hypothetical protein 4777121 4775133 5.3 70536 -<br />
5026 RB8966 2.16 1 660 1983 Chaperonin Cpn60/TCP-1 4777493 4779475 5.3 69819 +<br />
5144 RB9200 2.83 1 283 852 4-nitrophenylphosphatase PHO13 3.1.3.41 4919220 4918369 4.9 30413 -<br />
5173 RB9261 1.63 1 412 1239 conserved hypothetical protein 4952589 4953827 5 45911 +<br />
5228 RB9360 2.01 1 510 1533 Protein of unknown function, UPF0027 5015563 5014031 6.5 55186 -<br />
5241 RB9386 1.59 1 402 1209 secreted protein 5034937 5033729 4.2 44578 -<br />
5280 RB9460 1.93 1 79 240 hypothetical protein 5078860 5078621 10.3 8991 -<br />
5491 RB9852 1.94 1 223 672 RNA polymerase ECF-type sigma factor 5304982 5305653 5.7 25389 +<br />
5517 RB9897 1.82 1 74 225 hypothetical protein 5333773 5333549 7.6 8433 -
240h vs 82h down<br />
ID Locus Ratio AA Nuc Product Gene EC Start Stop IEP MW(kDa) Strand<br />
5576 RB10007 -2.950535456 313 942 conserved hypothetical protein 5390130 5391071 5 34128 +<br />
5577 RB10009 -1.972663257 127 384 similar to cold shock-like protein cspI 5391076 5391459 8.8 15428 +<br />
5618 RB10077 -4.910606182 88 267 secreted protein 5436817 5437083 3.3 9650 +<br />
5677 RB10178 -1.731163012 50 153 hypothetical protein 5502418 5502570 10.1 5494 +<br />
5682 RB10184 -1.588328294 93 282 hypothetical protein 5507891 5507610 4.7 10508 -<br />
5688 RB10199 -1.60726058 45 138 hypothetical protein 5512721 5512584 11.1 5010 -<br />
5702 RB10221 -1.496663605 402 1209 conserved hypothetical protein 5521594 5522802 5 45779 +<br />
5713 RB10242 -2.372898063 330 993 Curved DNA-binding protein cbpA 5536118 5535126 6.7 35177 -<br />
5722 RB10261 -4.163719815 81 246 Carbon storage regulator csrA 5545964 5545719 5.1 8704 -<br />
5733 RB10279 -3.629176353 151 456 heat shock protein, Hsp20 family 5557118 5557573 4.9 17072 +<br />
5734 RB10283 -3.799142749 107 324 small heat shock protein 5557737 5558060 4.7 12267 +<br />
5735 RB10286 -1.652654973 48 147 hypothetical protein 5558157 5558011 11.9 5389 -<br />
5763 RB10339 -1.892047037 519 1560 RNA polymerase sigma factor RpoD rpoD 5593398 5591839 10 60403 -<br />
5765 RB10342 -3.807868174 102 309 hypothetical protein 5593929 5593621 12.2 11300 -<br />
5785 RB10384 -3.181502151 96 291 hypothetical protein 5612174 5611884 4.1 10517 -<br />
556 RB1043 -2.148805836 89 270 conserved hypothetical protein 543083 543352 4.7 10039 +<br />
5815 RB10440 -1.999001876 49 150 hypothetical protein 5655693 5655842 11.5 5080 +<br />
5823 RB10458 -2.305925106 206 621 Rnpc2-prov protein 5671130 5670510 10.3 21043 -<br />
5871 RB10553 -1.753306 294 885 succinate dehydrogenase (cytochrome b558 subunit) sdhC 5723185 5724069 6 32847 +<br />
5910 RB10623 -1.852381909 60 183 secreted protein 5769140 5768958 3.9 6284 -<br />
5911 RB10627 -3.648306104 93 282 Chaperonin Cpn10 5769148 5769429 3.9 9966 +<br />
5939 RB10678 -1.571397244 116 351 hypothetical protein 5802466 5802816 12 13353 +<br />
5967 RB10727 -2.965232157 276 831 manganese-containing catalase 5830775 5831605 5 30055 +<br />
5968 RB10728 -1.908311366 132 399 secreted protein 5831649 5832047 9.9 13833 +<br />
5988 RB10755 -1.49616214 41 126 hypothetical protein 5839576 5839451 12.7 5083 -<br />
6043 RB10861 -3.483779094 46 141 hypothetical protein 5896851 5896991 4.2 4833 +<br />
6067 RB10896 -2.82978587 161 486 secreted protein 5917040 5916555 10 17034 -<br />
6084 RB10931 -3.454183366 54 165 hypothetical protein 5933742 5933906 4.2 5888 +<br />
6085 RB10932 -3.820050427 65 198 secreted protein 5933872 5934069 3.8 6983 +<br />
6086 RB10934 -4.349319572 68 207 CsbD-like 5934135 5934341 4.3 7929 +<br />
6094 RB10948 -4.571806624 153 462 conserved hypothetical protein, secreted 5938575 5939036 4.2 17070 +<br />
6095 RB10950 -2.285964397 233 702 membrane or secreted protein 5939124 5939825 4.9 25130 +<br />
6096 RB10951 -3.113701962 101 306 secreted protein 5940232 5939927 10.2 11754 -<br />
6098 RB10956 -3.206279336 117 354 hypothetical protein 5940336 5940689 4.8 13013 +<br />
6100 RB10958 -1.7585121 158 477 hypothetical protein 5941015 5941491 5.4 18101 +<br />
6106 RB10971 -1.740562506 303 912 Short-chain dehydrogenase/reductase SDR 5946406 5945495 9.8 32799 -<br />
6124 RB11000 -2.265626845 224 675 conserved hypothetical protein, membrane 5964054 5963380 8.8 24185 -<br />
6176 RB11107 -3.374113273 225 678 secreted protein 6021425 6022102 6.5 25080 +<br />
6217 RB11176 -3.194535765 153 462 protein containing DUF442 6065605 6065144 4.8 17161 -<br />
6244 RB11223 -1.530266559 120 363 ArsR family transcriptional regulator 6084224 6084586 10 13666 +<br />
6252 RB11235 -1.662941276 197 594 hypothetical protein 6090398 6090991 6.1 21804 +<br />
6256 RB11242 -1.973757083 201 606 RNA polymerase sigma-70 factor, ECF subfamily 6095778 6096383 7.6 22688 +<br />
6266 RB11260 -4.070997706 121 366 dnaK suppressor protein, 6107202 6106837 5.2 13639 -<br />
6346 RB11396 -1.511285155 36 111 conserved hypothetical protein 6178445 6178555 11.9 4104 +<br />
6347 RB11397 -2.503747863 207 624 SOUL heme-binding protein 6178540 6179163 8.7 23072 +<br />
6550 RB11731 -2.168186074 55 168 conserved hypothetical protein 6321063 6320896 10.6 6662 -<br />
6555 RB11742 -1.570721937 952 2859 conserved hypothetical protein, secreted 6323302 6326160 4.6 107208 +<br />
6601 RB11847 -1.971553443 243 732 Peptide methionine sulfoxide reductase msrA msrA 1.8.4.6 6379337 6380068 6.5 26839 +<br />
6611 RB11863 -2.03313839 375 1128 RecA bacterial DNA recombination protein 6388538 6387411 5.5 39958 -<br />
6623 RB11886 -1.845138428 933 2802 protein containing DUF1585 6403682 6400881 5.1 105437 -<br />
6708 RB12046 -3.474759639 51 156 conserved hypothetical protein 6497841 6497996 11.9 5529 +<br />
6714 RB12060 -1.591771433 142 429 conserved hypothetical protein 6507405 6506977 4.2 15422 -<br />
6718 RB12066 -4.196035054 135 408 hypothetical protein 6510147 6510554 10 14924 +<br />
6753 RB12137 -1.794707386 116 351 ATP-dependent Clp protease adaptor protein ClpS clpS 6550288 6550638 6.1 13341 +<br />
6760 RB12150 -1.53716483 53 162 conserved hypothetical protein 6556901 6557062 4.1 5983 +<br />
6766 RB12160 -4.183447848 108 327 thioredoxin trxA 6563104 6562778 4 11608 -<br />
6784 RB12197 -1.665469594 176 531 Initiation factor 3 6577659 6577129 10.2 20147 -<br />
6826 RB12266 -1.562865478 65 198 conserved hypothetical protein 6602558 6602755 12.1 7507 +<br />
6831 RB12276 -3.093532299 110 333 conserved hypothetical protein 6605112 6605444 4.7 11911 +<br />
6836 RB12283 -3.414193087 52 159 conserved hypothetical protein 6609220 6609378 7.6 5927 +<br />
6845 RB12296 -1.580558967 339 1020 Short-chain dehydrogenase/reductase SDR 6615422 6614403 10.1 36620 -<br />
6846 RB12297 -2.802982743 169 510 conserved hypothetical protein 6615598 6616107 4.4 17963 +<br />
6861 RB12323 -1.848909985 248 747 conserved hypothetical protein 6627001 6627747 4.4 27447 +<br />
6878 RB12348 -5.771592144 260 783 FxsA cytoplasmic membrane protein 6645520 6644738 11.6 28277 -<br />
6888 RB12372 -2.481519065 242 729 RNA polymerase ECF-type sigma factor 6658702 6659430 5.5 27478 +<br />
6904 RB12396 -1.657462567 57 174 hypothetical protein 6670870 6670697 11.3 6680 -<br />
6941 RB12454 -1.531429375 199 600 conserved hypothetical protein 6703647 6703048 10.3 22347 -<br />
6950 RB12471 -1.843727345 85 258 conserved hypothetical protein 6712442 6712185 9.7 9841 -<br />
7023 RB12610 -2.186910121 85 258 conserved hypothetical protein 6793700 6793957 9.7 9221 +<br />
7028 RB12621 -1.819879252 85 258 conserved hypothetical protein 6796861 6796604 8.9 9777 -<br />
7050 RB12658 -1.538038963 196 591 3-isopropylmalate dehydratase small subunit leuD 4.2.1.33 6814851 6815441 4.4 22377 +<br />
7096 RB12744 -1.582855123 36 111 conserved hypothetical protein 6868430 6868320 3.8 4043 -<br />
7150 RB12831 -1.7777588 264 795 Glycosyltransferase 6921326 6920532 4.6 29501 -<br />
7168 RB12861 -1.724523137 309 930 Oligopeptide transport system permease protein OppB. oppB 6932929 6932000 9.5 33415 -<br />
691 RB1291 -1.681127797 97 294 hypothetical protein 656684 656977 10.4 10290 +<br />
7278 RB13093 -2.422768724 158 477 secreted protein 7025577 7025101 9.6 17298 -<br />
7280 RB13095 -2.854720284 229 690 conserved hypothetical protein 7026753 7027442 7.7 24080 +<br />
7293 RB13115 -2.499869928 81 246 conserved hypothetical protein 7036614 7036369 9.8 8735 -<br />
7295 RB13117 -2.205679146 156 471 nitrogen regulation protein 7036595 7037065 4.6 17819 +<br />
703 RB1316 -1.56016378 184 555 membrane protein 664924 665478 7.3 19862 +<br />
7332 RB13186 -1.548743328 772 2319 subtilisin proteinase-like protein 7081960 7079642 6.1 86065 -<br />
7346 RB13214 -1.703514574 36 111 conserved hypothetical protein 7090176 7090286 9 3969 +<br />
7369 RB13257 -1.997869142 286 861 2-hydroxy-6-ketonona-2,4-dienedioic acid hydrolase bphD 3.7.1.- 7105590 7106450 4.6 32540 +<br />
7401 RB13324 -1.516645887 36 111 hypothetical protein 7141677 7141787 7.3 4100 +<br />
731 RB1359 -1.860782335 629 1890 Protease do precursor degP 3.4.21.- 693488 695377 6.1 66729 +<br />
79 RB138 -1.731437429 214 645 Sigma factor, ECF-like 60872 61516 4.8 24057 +<br />
746 RB1392 -1.845364991 340 1023 RNA polymerase sigma factor sigK 711705 710683 10.4 39326 -<br />
747 RB1394 -3.170631547 78 237 hypothetical protein 711994 711758 10.1 8765 -<br />
781 RB1461 -1.680149593 184 555 membrane protein 743517 742963 10 21051 -<br />
838 RB1561 -1.531156924 235 708 Ribulose-phosphate 3-epimerase rpe 5.1.3.1 798959 798252 4.3 25143 -<br />
869 RB1618 -1.579127747 97 294 hypothetical protein 833743 834036 5.3 11108 +<br />
883 RB1644 -4.895671621 123 372 secreted protein 845824 845453 9.7 13720 -<br />
915 RB1728 -2.530308084 285 858 membrane protein containing DUF1206 899954 900811 9.9 30055 +<br />
2 RB2 -1.548585963 59 180 secreted protein 182 3 3.7 6489 -<br />
1200 RB2274 -1.701113593 130 393 hypothetical protein 1193331 1192939 10 14362 -<br />
1236 RB2331 -1.510099259 133 402 hypothetical protein 1215088 1214687 10.3 15081 -<br />
1332 RB2479 -1.515017864 273 822 conserved hypothetical protein 1296683 1295862 5.5 30436 -<br />
1379 RB2543 -1.518521316 691 2076 30S ribosomal protein S1 rpsA 1326685 1328760 4.4 76492 +<br />
145 RB264 -1.784218682 64 195 Ribosomal protein S21 132677 132871 12.3 8074 +<br />
1434 RB2647 -2.035828497 257 774 conserved hypothetical protein, secreted 1374889 1374116 4.9 27907 -<br />
1435 RB2649 -2.484632275 53 162 hypothetical protein 1374987 1375148 9.1 5813 +<br />
1453 RB2680 -1.725932617 467 1404 conserved hypothetical protein, membrane 1393566 1392163 6.4 49834 -<br />
1454 RB2682 -3.204746944 83 252 hypothetical protein 1393781 1393530 7.4 8960 -<br />
1519 RB2810 -5.365744728 49 150 hypothetical protein 1459901 1459752 11.3 5618 -<br />
1566 RB2900 -3.217833661 90 273 hypothetical protein 1506927 1507199 9.1 9939 +<br />
1606 RB2963 -1.983225367 119 360 hypothetical protein 1526707 1527066 12.2 13440 +<br />
1613 RB2975 -1.621747024 133 402 Nitrogen regulatory protein P-II glnB 1535384 1534983 8.8 14941 -<br />
3 RB3 -1.505962523 73 222 hypothetical protein 39 260 8.9 8439 +<br />
1641 RB3023 -2.31685063 155 468 secreted protein 1566881 1566414 10 16924 -<br />
1734 RB3205 -1.630395388 76 231 hypothetical protein 1652079 1651849 11.3 9082 -<br />
1778 RB3281 -1.623405135 139 420 Histone-like bacterial DNA-binding protein 1687272 1686853 7.3 15802 -<br />
1835 RB3374 -2.36745013 95 288 hypothetical protein 1740350 1740063 11 11130 -<br />
1847 RB3399 -1.646194515 65 198 hypothetical protein 1753650 1753453 9.7 7418 -<br />
198 RB344 -1.667074676 700 2103 conserved hypothetical protein, secreted 173795 171693 5 77140 -
1967 RB3603 -1.519398413 344 1035 secreted protein 1875461 1874427 4.6 37999 -<br />
1984 RB3627 -1.69976521 280 843 Transglutaminase-like domain 1888482 1887640 4.5 30412 -<br />
2088 RB3789 -3.05899621 159 480 hypothetical protein 1962878 1962399 6.4 18218 -<br />
2098 RB3813 -1.586022583 75 228 hypothetical protein 1975188 1975415 4.5 8522 +<br />
2141 RB3882 -1.638277832 50 153 hypothetical protein 2007369 2007217 12.4 5918 -<br />
2154 RB3905 -1.769567638 38 117 hypothetical protein 2018601 2018717 5.1 4131 +<br />
2164 RB3923 -1.624470467 55 168 hypothetical protein 2032453 2032286 10.5 5956 -<br />
2168 RB3928 -1.546926335 173 522 hypothetical protein 2033789 2034310 10 19677 +<br />
2184 RB3953 -1.717863646 857 2574 hypothetical protein 2048784 2046211 5.2 93881 -<br />
2205 RB3989 -1.571221978 110 333 membrane protein 2060382 2060714 6.1 12440 +<br />
2210 RB3994 -1.62263894 191 576 hypothetical protein 2061291 2061866 4.1 20739 +<br />
234 RB402 -2.202205745 151 456 hypothetical protein 212820 213275 4.4 17306 +<br />
2247 RB4071 -1.932729384 369 1110 integral membrane protein 2098441 2099550 6.7 40439 +<br />
2260 RB4097 -1.760503575 733 2202 conserved hypothetical protein 2108244 2110445 6.2 83188 +<br />
2298 RB4165 -1.637930288 721 2166 hypothetical protein 2139632 2141797 4.5 77994 +<br />
241 RB417 -2.048581122 314 945 nonspecific nucleoside hydrolase 221324 222268 4.8 33970 +<br />
2304 RB4176 -3.424167784 96 291 hypothetical protein 2146928 2146638 12.4 11402 -<br />
242 RB418 -1.961141914 363 1092 conserved hypothetical protein, membrane 222222 223313 10.3 39119 +<br />
2314 RB4196 -1.517887215 114 345 hypothetical protein 2157140 2157484 9 12738 +<br />
2350 RB4255 -1.647794393 204 615 hypothetical protein 2180806 2180192 10.3 21582 -<br />
2351 RB4256 -1.700309632 358 1077 conserved hypothetical protein, secreted 2180303 2181379 4.5 37091 +<br />
248 RB430 -1.514532205 522 1569 protein containing DUF1501 229809 231377 6 58047 +<br />
2407 RB4347 -3.317265914 156 471 conserved hypothetical protein 2235735 2236205 4.7 17459 +<br />
2417 RB4360 -1.741651416 156 471 hypothetical protein 2240075 2239605 4.7 17592 -<br />
2437 RB4394 -3.867110768 188 567 protease I pfpI 3.2.-.- 2261372 2261938 4.4 20601 +<br />
2439 RB4396 -1.561295647 451 1356 glutathione reductase gor 1.8.1.7 2262532 2263887 5 48547 +<br />
2441 RB4398 -1.720721935 35 108 hypothetical protein 2264261 2264154 9 3825 -<br />
2443 RB4400 -3.078351438 58 177 hypothetical protein 2265382 2265206 9 6610 -<br />
2451 RB4416 -1.710492788 833 2502 Cytochrome c oxidase, subunit I 2271803 2269302 5.8 92293 -<br />
2461 RB4429 -3.079626574 89 270 conserved hypothetical protein 2275996 2276265 4.9 10034 +<br />
2462 RB4432 -2.017757176 268 807 glucose 1-dehydrogenase or 3-oxoacyl-[acyl-carrier-protein] reductase 1.1.1.- 2276333 2277139 4.7 28181 +<br />
2465 RB4438 -3.0073471 160 483 Pyridoxamine 5'-phosphate oxidase- 2278247 2278729 4.4 18043 +<br />
2472 RB4454 -1.784472046 739 2220 Flagellin A flaA 2283201 2285420 4 76924 +<br />
2477 RB4459 -1.720756801 462 1389 MscS Mechanosensitive ion channel 2288477 2287089 4.8 51157 -<br />
2481 RB4464 -3.330533712 223 672 secreted protein 2292075 2291404 5.1 25669 -<br />
2485 RB4468 -2.265372113 162 489 hypothetical protein 2294104 2293616 4.6 16380 -<br />
2486 RB4470 -3.659108285 169 510 secreted protein 2294676 2294167 4.9 19277 -<br />
2488 RB4474 -4.231918079 168 507 CsbD-like 2295048 2295554 8.8 18451 +<br />
2489 RB4476 -2.194667158 128 387 hypothetical protein 2295557 2295943 11.4 14037 +<br />
2490 RB4477 -2.465392744 69 210 hypothetical protein 2295940 2296149 10.6 7470 +<br />
2492 RB4480 -2.486728305 89 270 hypothetical protein 2296646 2296915 6.1 9442 +<br />
2498 RB4492 -1.813744209 101 306 hypothetical protein 2302138 2301833 9.9 10841 -<br />
2499 RB4493 -4.82841864 83 252 hypothetical protein 2302374 2302123 10.2 8978 -<br />
2500 RB4497 -2.781127188 49 150 hypothetical protein 2302950 2303099 10.3 5351 +<br />
2503 RB4500 -4.108372895 87 264 conserved hypothetical protein, secreted 2303645 2303908 3.5 9432 +<br />
2504 RB4502 -1.587856413 59 180 Protein of unknown function, UPF0057 2303992 2304171 9.1 6443 +<br />
2505 RB4504 -1.515612594 181 546 membrane protein containing DUF421 2304293 2304838 7.5 19859 +<br />
2524 RB4538 -1.598974592 172 519 secreted protein 2326219 2325701 5.4 18500 -<br />
2568 RB4613 -3.390322421 110 333 HesB/YadR/YfhF 2362510 2362178 4.6 12216 -<br />
2569 RB4614 -3.763789304 44 135 hypothetical protein 2362625 2362491 3.9 4907 -<br />
2584 RB4642 -2.739852866 85 258 conserved hypothetical protein 2377301 2377558 4.4 9855 +<br />
2607 RB4676 -1.862123889 221 666 hypothetical protein 2391079 2390414 10.4 23751 -<br />
2608 RB4678 -1.853751899 199 600 secreted protein 2390618 2391217 4.2 20927 +<br />
2657 RB4754 -2.122934062 58 177 hypothetical protein 2437589 2437765 6 6098 +<br />
280 RB479 -1.7608127 1101 3306 secreted protein 259174 255869 4.9 123358 -<br />
2693 RB4809 -2.337028238 98 297 membrane protein 2464977 2465273 10.1 10285 +<br />
2726 RB4867 -2.169969784 210 633 SmpB RNA-binding protein SmpB 2496354 2495722 10.8 23762 -<br />
2733 RB4876 -3.631304332 397 1194 delta 9 acyl-lipid fatty acid desaturase 1.14.19.- 2506214 2505021 9.5 44741 -<br />
2747 RB4900 -1.59067028 166 501 hypothetical protein 2520067 2519567 11.4 18298 -<br />
2770 RB4931 -1.666717737 217 654 secreted protein 2537481 2538134 5.9 24246 +<br />
2773 RB4936 -1.738166802 269 810 O-acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine O-acyltransferase lpxA 2.3.1.129 2539280 2540089 8.7 29110 +<br />
2784 RB4958 -1.77450529 250 753 hypothetical protein 2549283 2548531 9.8 27813 -<br />
289 RB497 -1.56741782 368 1107 methanol dehydrogenase regulatory protein; (moxR) 269443 270549 5.4 40725 +<br />
2816 RB5001 -1.64544186 103 312 hypothetical protein 2572354 2572665 10.3 11694 +<br />
2850 RB5064 -1.7367901 648 1947 secreted protein containing DUF1585 2605707 2607653 4.6 72145 +<br />
2875 RB5103 -1.67343724 352 1059 membrane protein 2622260 2623318 9.1 39152 +<br />
296 RB514 -2.436714418 56 171 hypothetical protein 284567 284397 10.2 6284 -<br />
2927 RB5196 -1.84638216 701 2106 trehalose synthase treS 5.4.99.16 2678058 2675953 5.6 81037 -<br />
300 RB520 -2.441328044 375 1128 conserved hypothetical protein 284989 286116 4.8 41300 +<br />
2963 RB5261 -1.777215676 126 381 hypothetical protein 2713712 2713332 7.4 13761 -<br />
2964 RB5262 -1.987732634 95 288 membrane protein 2713742 2714029 6.3 10993 +<br />
2978 RB5282 -1.563469412 578 1737 choline-sulfatase betC 3.1.6.6 2727443 2725707 5 65084 -<br />
3024 RB5373 -1.610866446 44 135 hypothetical protein 2775404 2775270 10.4 5046 -<br />
3100 RB5499 -1.718253529 447 1344 conserved hypothetical protein c 2844998 2843655 7.1 48835 -<br />
3107 RB5506 -1.763298362 51 156 hypothetical protein 2851102 2850947 10.3 6122 -<br />
3130 RB5551 -1.726697769 663 1992 hypothetical protein 2889101 2891092 5.7 70932 +<br />
3139 RB5567 -2.807682104 66 201 hypothetical protein 2897766 2897566 12.1 7106 -<br />
3162 RB5613 -1.718686676 360 1083 protein containing DUF1006 2921546 2922628 9.9 42148 +<br />
327 RB566 -2.408254421 294 885 exopolysaccharide synthesis, ExoD family 312007 311123 10 32340 -<br />
328 RB568 -1.54389448 77 234 hypothetical protein 312345 312112 10.2 8689 -<br />
3202 RB5681 -1.899201568 587 1764 Trigger factor tig 2965080 2966843 4.5 66546 +<br />
3219 RB5715 -1.852875395 210 633 hypothetical protein 2982826 2983458 4.9 24368 +<br />
3247 RB5761 -1.678461629 258 777 conserved hypothetical protein, membrane 3015202 3014426 10.9 27944 -<br />
3270 RB5801 -1.514537677 89 270 Ribosomal protein S15 3039583 3039852 10.6 10154 +<br />
3272 RB5804 -1.667295501 818 2457 polynucleotide phosphorylase 3040160 3042616 5.3 88202 +<br />
3294 RB5848 -4.212733889 140 423 dnaK suppressor protein, 3066328 3065906 4.6 15633 -<br />
3295 RB5850 -2.107814411 63 192 hypothetical protein 3066498 3066307 5.8 7096 -<br />
3316 RB5882 -2.135767975 243 732 hypothetical protein 3084330 3083599 9.8 27773 -<br />
3339 RB5924 -1.591642193 847 2544 hypothetical protein 3105828 3103285 5.2 92073 -<br />
3355 RB5955 -2.57021459 177 534 hypothetical protein 3121488 3122021 4.5 19364 +<br />
3389 RB6010 -1.641562068 53 162 hypothetical protein 3149911 3150072 11.9 5744 +<br />
3421 RB6071 -1.658689447 213 642 hypothetical protein 3187463 3188104 5.1 23869 +<br />
3465 RB6156 -1.525242339 134 405 membrane protein 3233026 3232622 9.5 14531 -<br />
3478 RB6178 -1.844910512 41 126 hypothetical protein 3240162 3240287 12.7 4414 +<br />
3547 RB6314 -2.653088765 52 159 hypothetical protein 3319320 3319478 6.3 6180 +<br />
3571 RB6351 -1.737859307 58 177 hypothetical protein 3336171 3336347 4.2 6411 +<br />
3587 RB6378 -1.986811448 228 687 hypothetical protein 3352598 3353284 12.3 25698 +<br />
3588 RB6379 -3.063507449 153 462 hypothetical protein 3353122 3352661 5.8 17398 -<br />
354 RB638 -1.810321183 436 1311 PQQ enzyme repeat domain protein 346447 347757 4.5 47685 +<br />
3605 RB6403 -3.371448655 304 915 hypothetical protein 3369939 3370853 5.4 33688 +<br />
3615 RB6417 -2.965326241 249 750 conserved hypothetical protein 3378045 3378794 5.9 27679 +<br />
3620 RB6428 -1.530633865 164 495 conserved hypothetical protein, secreted 3382816 3382322 6.5 17292 -<br />
3621 RB6429 -2.59203864 61 186 hypothetical protein 3382998 3382813 12.1 7095 -<br />
3644 RB6463 -1.646018383 189 570 conserved hypothetical protein, membrane 3415155 3415724 10.2 19818 +<br />
3674 RB6504 -1.754818069 53 162 hypothetical protein 3456120 3455959 9.6 5968 -<br />
3716 RB6578 -1.495239464 311 936 secreted protein 3495957 3495022 4.7 34703 -<br />
359 RB658 -1.69573478 412 1239 transport protein 356228 354990 5.2 44434 -<br />
3718 RB6583 -1.91791944 996 2991 secreted protein 3498012 3501002 4.5 112232 +<br />
3724 RB6595 -1.552798644 234 705 Rickettsia 17 kDa surface antigen 3506173 3505469 10.1 24227 -<br />
3768 RB6681 -1.549810836 139 420 hypothetical protein 3551775 3552194 4.9 15804 +<br />
3781 RB6701 -1.632497838 87 264 hypothetical protein 3559944 3560207 12 9863 +<br />
3795 RB6724 -1.861775884 131 396 hypothetical protein 3569189 3569584 10.5 14916 +<br />
3797 RB6728 -1.553174354 113 342 hypothetical protein 3570581 3570922 9.8 12410 +<br />
3820 RB6766 -1.498615661 55 168 hypothetical protein 3597367 3597534 12 6133 +<br />
3827 RB6778 -2.494952322 59 180 hypothetical protein 3603142 3603321 5.5 6533 +<br />
374 RB684 -1.537572032 653 1962 Arylsulfatase precursor atsA 3.1.6.1 368557 366596 5.5 73598 -
3871 RB6856 -1.574937983 62 189 hypothetical protein 3645613 3645425 9.9 6382 -<br />
3890 RB6895 -1.624779575 401 1206 Glycosyl transferase, family 4 3666358 3665153 6.6 42272 -<br />
3900 RB6910 -3.124391756 42 129 hypothetical protein 3671484 3671356 9.5 4575 -<br />
3901 RB6911 -3.192466719 430 1293 protein containing DUF1559 3671445 3672737 6 45752 +<br />
3902 RB6912 -1.79865869 55 168 secreted protein 3672793 3672960 9 5999 +<br />
3908 RB6923 -1.699483974 171 516 hypothetical protein 3677883 3678398 4.4 17942 +<br />
3917 RB6941 -1.666439291 199 600 secreted protein 3686675 3686076 6.2 21829 -<br />
3959 RB7022 -1.796981564 90 273 Ribosomal protein S20 3735291 3735563 12.3 10255 +<br />
3969 RB7039 -1.693951445 202 609 hypothetical protein 3743573 3742965 7.9 23105 -<br />
3993 RB7085 -4.428779467 75 228 hypothetical protein 3763662 3763435 10.4 8270 -<br />
4022 RB7129 -2.299584218 76 231 iron-dependent transcriptional repressor 3782388 3782618 9.1 8059 +<br />
4109 RB7279 -2.03956414 96 291 hypothetical protein 3870019 3870309 6 10990 +<br />
4120 RB7296 -1.660060013 82 249 hypothetical protein 3877224 3877472 11.7 9406 +<br />
43 RB73 -1.564568283 98 297 membrane protein 28727 29023 10.1 11150 +<br />
4130 RB7312 -1.645528305 54 165 hypothetical protein 3891868 3891704 12.1 5909 -<br />
4131 RB7313 -2.518930041 51 156 hypothetical protein 3892097 3891942 10.5 5558 -<br />
4197 RB7452 -2.490276153 170 513 conserved hypothetical protein 3994713 3995225 5.7 19622 +<br />
4225 RB7507 -1.59774427 521 1566 conserved hypothetical protein, membrane 4036748 4038313 5.1 57952 +<br />
406 RB758 -1.510478448 72 219 hypothetical protein 407558 407340 9.9 8100 -<br />
4278 RB7589 -1.664248577 86 261 hypothetical protein 4080238 4079978 12.1 9472 -<br />
4291 RB7612 -1.816717055 136 411 hypothetical protein 4092907 4093317 11.4 15607 +<br />
4308 RB7634 -1.65869293 773 2322 Abortive infection protein 4106267 4108588 6 84772 +<br />
4315 RB7647 -5.223347511 73 222 hypothetical protein 4111931 4112152 7.4 8269 +<br />
4327 RB7667 -2.594139449 38 117 hypothetical protein 4117097 4116981 6.7 4315 -<br />
4357 RB7722 -1.666327346 278 837 membrane protein 4137251 4136415 9.7 30699 -<br />
4413 RB7818 -2.422254757 121 366 Ribosomal protein S12/S23 4185730 4186095 11.8 13642 +<br />
4474 RB7914 -1.518244974 199 600 hypothetical protein 4228476 4227877 9.8 20970 -<br />
4484 RB7937 -1.526677279 41 126 hypothetical protein 4237809 4237684 10.7 4936 -<br />
4494 RB7955 -1.502357171 123 372 hypothetical protein 4247114 4246743 5.1 14227 -<br />
4503 RB7978 -1.504930954 114 345 hypothetical protein 4265473 4265817 12.7 12583 +<br />
4519 RB8012 -1.534648135 129 390 hypothetical protein 4282669 4282280 10.5 14076 -<br />
4526 RB8027 -1.51323771 232 699 Sigma factor, ECF-like 4289305 4290003 5.5 26899 +<br />
4530 RB8031 -2.199787647 396 1191 6-phosphogluconolactonase pgl 3.1.1.31 4295373 4294183 5.8 42309 -<br />
4531 RB8034 -1.58729228 147 444 membrane protein 4295344 4295787 10.2 16546 +<br />
4549 RB8066 -1.505494445 38 117 hypothetical protein 4318635 4318519 6.3 4358 -<br />
4589 RB8144 -6.015829652 143 432 hypothetical protein 4354622 4354191 10.1 16269 -<br />
4590 RB8146 -2.207593403 151 456 hypothetical protein 4355122 4354667 4.3 17016 -<br />
4591 RB8149 -1.930738553 70 213 Carbon storage regulator csrA 4355339 4355551 5.6 7884 +<br />
4628 RB8226 -1.677165624 104 315 hypothetical protein 4400097 4399783 4.7 11488 -<br />
4629 RB8227 -1.896205229 59 180 hypothetical protein 4400365 4400186 9.3 6533 -<br />
4644 RB8253 -1.684893885 592 1779 ASPARTYL-TRNA SYNTHETASE ASPS (ASPARTATE--TRNA aspS 6.1.1.12 4410353 4412131 4.8 66195 +<br />
4645 RB8254 -1.658128547 729 2190 amino acid transporter, permease 4412322 4414511 10.1 77104 +<br />
4677 RB8319 -1.831492165 37 114 hypothetical protein 4442421 4442308 4.4 4328 -<br />
4689 RB8337 -1.520816995 699 2100 hypothetical protein 4454547 4452448 4.8 77373 -<br />
4690 RB8342 -1.573979225 43 132 hypothetical protein 4454696 4454565 10 4601 -<br />
4698 RB8356 -1.731193223 69 210 hypothetical protein 4459802 4460011 12.3 7927 +<br />
4758 RB8473 -1.610739411 41 126 hypothetical protein 4523410 4523535 9 4372 +<br />
466 RB861 -1.557762906 499 1500 conserved hypothetical protein, membrane 457825 456326 5.6 54035 -<br />
4847 RB8633 -2.655123844 464 1395 Aminotransferase class-III 2.6.1.- 4601126 4602520 5.6 50436 +<br />
4892 RB8725 -1.731814086 54 165 50S ribosomal protein L33 rpmG 4638301 4638465 10.6 6465 +<br />
52 RB88 -1.756791964 470 1413 phosphodiesterase/alkaline phosphatase 34254 35666 4.8 53751 +<br />
4937 RB8807 -1.615250042 411 1236 membrane protein containing DUF502 4686313 4685078 9.5 45282 -<br />
5006 RB8921 -1.566810255 393 1182 conserved hypothetical protein, membrane 4751930 4750749 9.9 43096 -<br />
5026 RB8966 -2.915090463 660 1983 Chaperonin Cpn60/TCP-1 4777493 4779475 5.3 69819 +<br />
5027 RB8969 -1.548585963 101 306 Chaperonin Cpn10 4779554 4779859 4.9 10628 +<br />
5046 RB9011 -1.54245952 355 1068 secreted protein 4800960 4802027 8.8 38071 +<br />
5093 RB9104 -1.72739241 45 138 hypothetical protein 4866696 4866559 9 4979 -<br />
5094 RB9105 -3.701526149 704 2115 Heat shock protein Hsp70 dnaK 4868834 4866720 4.6 76038 -<br />
5106 RB9126 -1.851222433 54 165 hypothetical protein 4885253 4885417 10.4 6196 +<br />
5113 RB9141 -1.510999263 346 1041 rRNA (guanine-N(2)-)-methyltransferase 4891836 4892876 7.8 38276 +<br />
5144 RB9200 -1.585069921 283 852 4-nitrophenylphosphatase PHO13 3.1.3.41 4919220 4918369 4.9 30413 -<br />
5155 RB9227 -1.719216359 105 318 hypothetical protein 4934649 4934332 6.7 11414 -<br />
5193 RB9304 -3.286314686 81 246 Ribosomal protein L31 4974378 4974623 10.1 9068 +<br />
5205 RB9324 -1.673298626 267 804 secreted protein 4992474 4993277 6.7 29463 +<br />
5215 RB9344 -1.630389285 1386 4161 probable swi/snf family helicase 2 5003979 5008139 5.6 153977 +<br />
5243 RB9389 -2.197015543 130 393 hypothetical protein 5035390 5034998 11.6 14637 -<br />
5245 RB9392 -1.857212458 175 528 hypothetical protein 5038107 5037580 4.9 19499 -<br />
5275 RB9445 -1.846433581 249 750 integral membrane protein 5074872 5075621 10.4 26862 +<br />
5277 RB9448 -1.693010753 115 348 conserved hypothetical membrane protein 5076267 5076614 7.4 12838 +<br />
5292 RB9484 -1.508990361 519 1560 conserved hypothetical protein 5094011 5092452 6.3 57312 -<br />
516 RB951 -2.181294685 234 705 protein containing DUF1596 502459 501755 12.3 25583 -<br />
5313 RB9520 -1.638871436 532 1599 conserved hypothetical protein 5114495 5112897 4.4 59231 -<br />
5318 RB9529 -1.658070617 113 342 hypothetical protein 5117956 5118297 9.3 13082 +<br />
5345 RB9574 -1.634265363 268 807 Short-chain dehydrogenase/reductase SDR 1.-.-.- 5150355 5149549 5 28959 -<br />
5348 RB9581 -2.386454842 128 387 protein containing DUF24 5150921 5150535 8.7 14614 -<br />
5353 RB9588 -1.838366822 268 807 conserved hypothetical protein 5156821 5156015 5.6 29455 -<br />
5362 RB9610 -1.705062421 501 1506 NAD-dependent epimerase/dehydratase 5169123 5170628 6.2 56132 +<br />
5386 RB9651 -1.601623777 544 1635 sialic acid-specific 9-O-acetylesterase 3.1.1.53 5198277 5196643 5.9 60120 -<br />
5390 RB9657 -1.523443287 359 1080 OXIDOREDUCTASE PROTEIN 5202179 5203258 4.8 39151 +<br />
5411 RB9696 -1.827291105 633 1902 Protein of unknown function DUF879, bacterial 5223290 5225191 6.4 70360 +<br />
5419 RB9713 -1.501753835 1082 3249 large, multifunctional secreted protein 5243297 5240049 4.9 120210 -<br />
5434 RB9745 -1.598772986 656 1971 serine/threonine protein kinase pkn2 2.7.1.37 5252442 5254412 5.2 72146 +<br />
5445 RB9767 -1.562821415 97 294 hypothetical protein 5264348 5264641 8.9 10547 +<br />
5469 RB9806 -1.763185009 165 498 membrane protein 5280590 5281087 10.2 18166 +<br />
5473 RB9811 -1.60581527 102 309 secreted protein 5284365 5284673 11.7 11169 +<br />
5474 RB9813 -2.144492962 161 486 hypothetical protein 5285123 5284638 12 17657 -<br />
5480 RB9830 -2.028072773 76 231 hypothetical protein 5293341 5293111 9.5 8437 -<br />
5520 RB9902 -1.61739367 178 537 secreted protein 5336299 5335763 3.6 19680 -<br />
5533 RB9920 -1.519562977 140 423 Ribosomal protein S6 5344827 5344405 4.3 15428 -<br />
5538 RB9930 -4.203188124 118 357 hypothetical protein 5349331 5348975 4 12809 -<br />
5552 RB9959 -2.486765443 65 198 hypothetical protein 5366417 5366220 11.9 7376 -<br />
5571 RB9999 -2.415288141 281 846 conserved hypothetical protein-putative transcriptional regulator 5386828 5385983 4.6 29948 -
240h vs 82h up<br />
ID Locus Ratio AA Nuc Product Gene EC Start Stop IEP MW(kDa) Strand<br />
58 RB100 1.654514659 123 372 membrane protein 41097 40726 7.5 13930 -<br />
5578 RB10010 4.373631074 840 2523 secreted protein containing DUF1585 5393982 5391460 4.9 95249 -<br />
5579 RB10014 2.042872232 534 1605 protein containing DUF1552 5395577 5393973 7.2 58534 -<br />
5582 RB10020 2.189639697 64 195 hypothetical protein 5396788 5396594 9.1 7272 -<br />
5584 RB10022 1.605837782 165 498 membrane protein 5396916 5397413 6.3 17893 +<br />
5588 RB10026 2.159526021 883 2652 conserved hypothetical protein 5401902 5399251 6.5 98323 -<br />
5608 RB10053 1.579968872 496 1491 secreted protein containing DUF1501 5423854 5425344 6.6 54820 +<br />
5625 RB10096 2.67333667 341 1026 Transposase IS116/IS110/IS902 5446891 5445866 10 38283 -<br />
5627 RB10098 1.885841688 47 144 hypothetical protein 5447310 5447453 9.7 5129 +<br />
5631 RB10105 1.531582074 115 348 protein containing DUF1582 5451202 5450855 8.9 12499 -<br />
5633 RB10107 1.628209869 50 153 hypothetical protein 5451876 5451724 9.1 5550 -<br />
5642 RB10119 1.627089735 766 2301 serine/threonine protein kinase pknD 2.7.1.37 5462723 5460423 5.3 82693 -<br />
5647 RB10126 4.215826536 43 132 hypothetical protein 5465210 5465341 12.9 4964 +<br />
5670 RB10167 1.676202415 59 180 hypothetical protein 5497096 5496917 9 6356 -<br />
5673 RB10172 1.509922517 489 1470 aldehyde dehydrogenase family protein (Betaine) 1.2.1.36 5499799 5498330 5 53366 -<br />
5711 RB10238 1.71830836 577 1734 protein containing DUF1559 5531834 5533567 5.8 62799 +<br />
5715 RB10245 2.200657651 328 987 haloalkane dehalogenase dhmA2 3.8.1.5 5537951 5538937 7.7 36310 +<br />
5716 RB10246 2.57631347 316 951 Triphosphoribosyl-dephospho-CoA protein (ORF7) citG 5538891 5539841 5.1 34552 +<br />
5718 RB10252 2.292741304 386 1161 hypothetical protein 5541386 5542546 4.3 42018 +<br />
5719 RB10255 3.701967215 337 1014 MscS Mechanosensitive ion channel 5542656 5543669 5.4 35647 +<br />
5720 RB10256 3.225664198 268 807 hypothetical protein 5543753 5544559 4.1 30801 +<br />
5721 RB10258 3.223167304 289 870 Survival protein SurE 3.1.3.2 5544584 5545453 5.3 31584 +<br />
5732 RB10277 2.90124719 476 1431 Pyruvate kinase pykF 2.7.1.40 5555414 5556844 5.2 52151 +<br />
5737 RB10290 2.928019934 142 429 hypothetical protein 5559631 5559203 12.4 16122 -<br />
5739 RB10295 1.699754772 308 927 methylene tetrahydrofolate/ mtdC 5562427 5561501 5.2 32122 -<br />
5745 RB10308 1.548630277 308 927 response regulator 5569131 5570057 4.9 34171 +<br />
5750 RB10319 3.610640028 65 198 hypothetical protein 5577574 5577771 9.6 7480 +<br />
5756 RB10328 2.940635557 227 684 protein containing DUF633 5583675 5582992 9.5 25921 -<br />
5762 RB10338 2.43243988 486 1461 TPR domain protein 5591839 5590379 5 53945 -<br />
5770 RB10347 1.690703459 184 555 hypothetical protein 5596258 5595704 5.5 20536 -<br />
5804 RB10419 1.500890259 40 123 hypothetical protein 5639506 5639384 12.1 4388 -<br />
5807 RB10427 1.50099123 47 144 hypothetical protein 5647025 5646882 12.1 5635 -<br />
5811 RB10433 1.722495307 441 1326 membrane protein 5651746 5650421 9.9 48642 -<br />
5880 RB10569 1.581605225 388 1167 protein containing DUF1559 5736174 5735008 5.4 41686 -<br />
5902 RB10608 2.057744599 457 1374 transposase and inactivated derivative 5759903 5761276 8.4 52492 +<br />
5903 RB10612 1.886271614 492 1479 choline-sulfatase betC 3.1.6.6 5761454 5762932 5.8 54603 +<br />
5942 RB10682 1.654794846 474 1425 secreted protein 5807422 5805998 12.2 53138 -<br />
5947 RB10689 1.616678827 85 258 hypothetical protein 5808207 5807950 12.2 9609 -<br />
5953 RB10698 1.843186951 150 453 hypothetical protein 5813025 5812573 5.8 16611 -<br />
5974 RB10735 1.978886422 332 999 hypothetical protein 5834722 5835720 5.3 37343 +<br />
5985 RB10752 1.904220821 42 129 hypothetical protein 5838740 5838612 10.7 4817 -<br />
5992 RB10764 1.988421087 195 588 hypothetical protein 5845271 5844684 8.6 22760 -<br />
6018 RB10816 1.863339078 376 1131 DNA polymerase III tau and gamma subunits 5875071 5873941 8.6 40800 -<br />
6040 RB10855 1.989745932 395 1188 mandelate racemase/muconate lactonizing enzyme family protein 5895378 5894191 6.4 43363 -<br />
6046 RB10866 3.289082377 82 249 hypothetical protein 5899291 5899043 10.2 9214 -<br />
6048 RB10868 2.008192745 136 411 hypothetical protein 5900330 5899920 11.3 15100 -<br />
6052 RB10876 1.853661907 839 2520 excinuclease ABC subunit A 5903770 5901251 6.4 90046 -<br />
6064 RB10893 2.84725917 57 174 hypothetical protein 5914894 5915067 9.7 6059 +<br />
580 RB1095 1.805808757 525 1578 hypothetical protein 567469 569046 4.5 57051 +<br />
6101 RB10960 1.540150809 139 420 conserved hypothetical protein, membrane 5941602 5942021 11.4 15743 +<br />
6102 RB10965 1.700480847 204 615 conserved hypothetical protein, membrane 5942116 5942730 4.9 21483 +<br />
581 RB1097 1.579125526 404 1215 membrane protein 570281 569067 5.3 42931 -<br />
6109 RB10978 1.591971636 122 369 Small multidrug resistance protein 5947515 5947883 4.6 12506 +<br />
6125 RB11001 3.10014494 294 885 conserved hypothetical protein 5964931 5964047 9.6 33860 -<br />
6128 RB11009 3.753956045 238 717 sulfite reductase-like flavoprotein 5969752 5969036 3.9 25651 -<br />
6129 RB11010 2.909442479 278 837 conserved hypothetical protein, secreted 5970585 5969749 4.9 30852 -<br />
6130 RB11011 2.956454785 181 546 conserved hypothetical protein, secreted 5971127 5970582 9.5 20389 -<br />
6131 RB11013 2.913287746 218 657 conserved hypothetical protein, membrane 5971802 5971146 4.9 24214 -<br />
6136 RB11023 1.809482834 59 180 hypothetical protein 5975603 5975424 3.8 6205 -<br />
6140 RB11031 2.682575659 62 189 hypothetical protein 5980865 5981053 12.1 7649 +<br />
6142 RB11036 1.911367719 470 1413 two-component system, regulatory protein 5985609 5984197 5.3 51036 -<br />
6143 RB11037 1.859310972 493 1482 two-component system sensor protein 5987098 5985617 5.8 55133 -<br />
6152 RB11062 1.687148515 214 645 conserved hypothetical protein 5995358 5994714 4.7 23983 -<br />
6157 RB11070 2.836648643 52 159 hypothetical protein 6000321 6000479 10.2 5582 +<br />
6165 RB11082 2.919691689 112 339 hypothetical protein 6007371 6007033 10.9 12552 -<br />
6206 RB11156 1.841373417 350 1053 secreted protein 6056074 6055022 5.8 38283 -<br />
6212 RB11168 2.882446961 149 450 thiosulfate sulfurtransferase 6062177 6061728 9.8 17041 -<br />
591 RB1120 1.607182316 651 1956 multidrug efflux protein, outer membrane component 576988 575033 4.7 72081 -<br />
6248 RB11228 2.001536123 42 129 hypothetical protein 6087599 6087471 13 5329 -<br />
6251 RB11233 2.219312927 295 888 hypothetical protein 6090340 6089453 4.7 33694 -<br />
6269 RB11268 1.86099064 97 294 hypothetical protein 6109768 6109475 10.8 11235 -<br />
6285 RB11293 1.562813644 97 294 hypothetical protein 6121182 6120889 10.3 11032 -<br />
6292 RB11303 1.816331576 464 1395 Transposase IS66 6128599 6127205 10.2 51739 -<br />
6321 RB11348 2.306868407 151 456 conserved hypothetical protein 6155692 6155237 3.4 16759 -<br />
6324 RB11356 1.621754315 237 714 glycosyltransferase, family 2 6158199 6157486 7.1 27585 -<br />
599 RB1138 2.497575401 155 468 conserved hypothetical protein 585492 585959 10.5 17130 +<br />
6362 RB11424 3.968781865 158 477 conserved hypothetical protein 6195656 6196132 11.1 17372 +<br />
6363 RB11427 2.669809078 93 282 conserved hypothetical protein 6196136 6196417 9.7 10315 +<br />
6364 RB11429 4.836258644 37 114 conserved hypothetical protein 6196478 6196591 12.4 4245 +<br />
6371 RB11439 4.771980115 93 282 conserved hypothetical protein 6197993 6198274 10.6 10906 +<br />
6382 RB11446 4.373489974 113 342 conserved hypothetical protein 6199492 6199833 10 12579 +<br />
6385 RB11450 3.292516777 60 183 conserved hypothetical protein 6200046 6200228 11.8 6517 +<br />
6399 RB11462 2.292414397 129 390 CoA-binding protein 6202949 6203338 5.1 13908 +<br />
6401 RB11468 1.600332302 239 720 conserved hypothetical protein, secreted 6205637 6204918 4.9 26639 -<br />
6411 RB11484 1.731982898 43 132 conserved hypothetical protein 6212742 6212611 5.5 4566 -<br />
6479 RB11616 2.050443667 56 171 conserved hypothetical protein 6262622 6262452 5.5 6002 -<br />
6480 RB11617 2.653050908 297 894 conserved hypothetical protein 6263689 6262796 5.5 33558 -<br />
6497 RB11641 2.148369724 297 894 conserved hypothetical protein 6278514 6277621 5.5 33558 -<br />
6508 RB11663 1.781211968 450 1353 sugar transport protein 6290653 6292005 9.6 48415 +<br />
6521 RB11677 2.207148141 47 144 conserved hypothetical protein 6300221 6300078 10.9 5108 -<br />
6524 RB11680 1.902883532 59 180 conserved hypothetical protein 6300635 6300456 10.1 6549 -<br />
6528 RB11685 1.624754627 59 180 conserved hypothetical protein 6301256 6301077 10.2 6734 -<br />
66 RB117 1.845310737 129 390 membrane or secreted protein 51525 51136 11 14217 -<br />
6544 RB11720 1.526186688 264 795 conserved hypothetical protein 6316234 6317028 10.7 27475 +<br />
6545 RB11722 1.764001072 342 1029 conserved hypothetical protein 6317037 6318065 6.7 37460 +<br />
6547 RB11728 1.859522593 295 888 D-Tagatose 3-epimerase 5.3.1.- 6318857 6319744 4.6 32417 +<br />
6548 RB11729 2.095879613 85 258 conserved hypothetical protein 6320009 6319752 10.6 9507 -<br />
6559 RB11750 2.072392265 292 879 integrase 6328357 6329235 10 33354 +<br />
6571 RB11768 2.488324614 43 132 conserved hypothetical protein 6334808 6334939 9.4 4603 +<br />
6572 RB11769 1.550502675 8173 24522 domain/calx-beta domain protein 6335006 6359527 3 826151 +<br />
622 RB1179 1.836873669 128 387 hypothetical protein 603998 604384 12 13863 +<br />
623 RB1180 1.682087161 92 279 hypothetical protein 604378 604656 12.6 9917 +<br />
6589 RB11823 1.936606455 634 1905 conserved hypothetical protein, secreted 6371378 6373282 5 68778 +<br />
6590 RB11826 1.664574845 100 303 conserved hypothetical protein 6373298 6373600 12.3 11008 +<br />
626 RB1184 1.629663004 98 297 protein containing DUF1556 605971 605675 11.4 10926 -<br />
6598 RB11844 1.69066366 94 285 conserved hypothetical protein 6378510 6378794 6.8 10448 +<br />
6605 RB11854 1.570100857 77 234 conserved hypothetical protein 6383404 6383171 13.5 8576 -
6606 RB11855 2.19926923 101 306 conserved hypothetical protein 6383765 6383460 12.5 11384 -<br />
6610 RB11862 1.6336847 56 171 conserved hypothetical protein, secreted 6387327 6387157 12 6073 -<br />
629 RB1190 1.531303399 292 879 integrase 607131 608009 10 33354 +<br />
630 RB1191 2.621249142 126 381 hypothetical transposase 608435 608055 9.6 13925 -<br />
6660 RB11951 3.121435039 195 588 conserved hypothetical protein 6437923 6438510 6.1 21768 +<br />
6670 RB11973 1.521276023 273 822 conserved hypothetical protein, membrane 6447543 6446722 10.1 28672 -<br />
6689 RB12006 1.721932414 341 1026 regulatory subunit 6475429 6476454 4.4 36562 +<br />
6691 RB12009 1.689190216 514 1545 conserved hypothetical protein 6479495 6477951 4.8 54935 -<br />
638 RB1201 2.499019821 41 126 hypothetical protein 612754 612629 12.5 4691 -<br />
6694 RB12015 1.614312795 321 966 conserved hypothetical protein 6483388 6482423 5.9 34513 -<br />
6701 RB12031 1.954623073 146 441 conserved hypothetical protein 6490799 6490359 9 16668 -<br />
6711 RB12053 1.729367164 119 360 biopolymer transport protein, ExbD/TolR family 6502558 6502199 4.2 12926 -<br />
6712 RB12055 2.850799464 319 960 MotA/TolQ/ExbB proton channel family protein 6503587 6502628 5.3 34206 -<br />
6720 RB12068 2.037532136 553 1662 serine/threonine protein kinase 6512255 6510594 8.5 61271 -<br />
6727 RB12080 1.62139257 492 1479 Protein-L-isoaspartate(D-aspartate) O-methyltransferase 2.1.1.77 6517231 6515753 8.9 53385 -<br />
644 RB1210 2.291039759 281 846 AP endonuclease 2, C-terminal 617416 616571 4.5 30665 -<br />
6745 RB12116 1.528505705 355 1068 DNA polymerase III epsilon subunit 6541348 6540281 9.2 38668 -<br />
6786 RB12200 1.907346776 51 156 conserved hypothetical protein 6577955 6577800 11 5747 -<br />
6800 RB12219 1.829206519 472 1419 conserved hypothetical protein 6586411 6584993 6.4 53798 -<br />
652 RB1223 2.353571948 73 222 hypothetical protein 624091 624312 10.4 7925 +<br />
6812 RB12239 2.627303086 433 1302 ISXo8 transposase 6593334 6594635 9.4 49646 +<br />
6815 RB12243 2.005776569 55 168 conserved hypothetical protein 6596256 6596423 10.5 5848 +<br />
654 RB1225 1.555906229 834 2505 dipeptidyl peptidase IV DPP4 3.4.14.5 627017 624513 4.8 93242 -<br />
6825 RB12265 1.506648506 42 129 conserved hypothetical protein 6602530 6602402 10.4 4412 -<br />
6833 RB12279 1.845574656 337 1014 MscS Mechanosensitive ion channel 6606558 6605545 4.5 36901 -<br />
6838 RB12286 1.697767005 57 174 conserved hypothetical protein 6610293 6610120 10.9 6473 -<br />
6844 RB12295 1.549620958 86 261 conserved hypothetical protein 6614190 6614450 9.1 9350 +<br />
6890 RB12377 4.034629067 284 855 Ribosomal RNA adenine methylase transferase 6661963 6661109 5.7 31560 -<br />
6891 RB12379 3.873999592 240 723 riboflavin synthase, alpha subunit 6662808 6662086 4.6 25728 -<br />
6894 RB12383 2.261031645 88 267 conserved hypothetical protein 6664767 6664501 3.9 9433 -<br />
6896 RB12386 1.963666988 61 186 conserved hypothetical protein, secreted 6665667 6665852 11.2 6546 +<br />
6897 RB12387 2.351753993 50 153 conserved hypothetical protein 6665894 6665742 10.1 5841 -<br />
6901 RB12392 2.308597949 35 108 conserved hypothetical protein 6668543 6668436 10.5 3832 -<br />
6902 RB12393 2.22524548 668 2007 c-type cytochrome precursor 6670561 6668555 5.9 74121 -<br />
6905 RB12398 1.713211441 361 1086 conserved hypothetical protein 6672013 6670928 10.1 39805 -<br />
663 RB1240 1.521961995 504 1515 protein containing DUF1080 634060 632546 9.4 55473 -<br />
6907 RB12403 2.082915079 59 180 conserved hypothetical protein 6673741 6673562 7.3 6301 -<br />
6908 RB12404 2.145144818 97 294 conserved hypothetical protein 6674021 6673728 12.2 10758 -<br />
6915 RB12416 2.659967583 292 879 secreted protein 6679578 6680456 6 31935 +<br />
6921 RB12427 2.178493515 56 171 conserved hypothetical protein 6684645 6684475 12.8 6556 -<br />
6930 RB12439 1.835694179 44 135 protein containing DUF1586 6695571 6695437 10.7 4885 -<br />
6933 RB12442 1.766365352 511 1536 conserved hypothetical protein 6698977 6697442 4.4 55156 -<br />
6954 RB12479 2.301385379 190 573 conserved hypothetical protein 6717557 6716985 5.8 21665 -<br />
6957 RB12484 1.921747867 324 975 conserved hypothetical protein, membrane 6721052 6720078 10.6 34787 -<br />
668 RB1254 1.631322418 45 138 hypothetical protein 640610 640747 10.6 4883 +<br />
6989 RB12543 1.623659414 331 996 conserved hypothetical protein, membrane 6759514 6758519 9.6 37564 -<br />
669 RB1255 1.967180069 51 156 hypothetical protein 640910 640755 12.3 5933 -<br />
6994 RB12554 2.448543373 71 216 conserved hypothetical protein 6764163 6764378 10.2 7146 +<br />
7045 RB12648 1.928441091 69 210 conserved hypothetical protein 6810287 6810496 11.2 7886 +<br />
677 RB1266 1.583975133 99 300 hypothetical protein 648804 648505 11.8 11071 -<br />
7066 RB12684 1.536286714 54 165 conserved hypothetical protein 6831038 6830874 5.4 5628 -<br />
7069 RB12689 2.115580227 106 321 secreted protein 6833004 6833324 9.8 11239 +<br />
678 RB1269 1.855473031 203 612 hypothetical protein 649412 648801 11.9 22612 -<br />
7070 RB12690 1.932274176 250 753 3-deoxy-manno-octulosonate cytidylyltransferase kdsB 2.7.7.38 6834088 6833336 4.7 27384 -<br />
7074 RB12694 1.792575761 343 1032 serine/threonine-protein kinase pknB 2.7.1.37 6837178 6836147 9.6 37011 -<br />
7081 RB12710 1.734953496 36 111 conserved hypothetical protein 6846593 6846703 9.2 4110 +<br />
7084 RB12716 1.68107695 114 345 conserved hypothetical protein 6849459 6849115 12.4 13128 -<br />
7088 RB12727 2.18182508 680 2043 conserved hypothetical protein 6860301 6858259 5.7 73171 -<br />
7092 RB12737 2.385652769 65 198 conserved hypothetical protein 6863917 6864114 9.1 6753 +<br />
7093 RB12739 2.910582075 56 171 conserved hypothetical protein 6864276 6864106 10.8 6057 -<br />
7119 RB12782 3.953832549 55 168 conserved hypothetical protein 6889986 6889819 10.9 5879 -<br />
7132 RB12806 1.69219261 205 618 conserved hypothetical protein 6911165 6910548 10.5 21914 -<br />
7134 RB12810 2.10152585 67 204 conserved hypothetical protein 6912339 6912542 11.3 7443 +<br />
7139 RB12815 2.403965196 43 132 conserved hypothetical protein 6914656 6914525 10.8 4782 -<br />
7140 RB12816 1.596657606 257 774 glutamine amidotransferase or related peptidase 6914710 6915483 4.5 28223 +<br />
7141 RB12818 4.913335797 163 492 conserved hypothetical protein 6915989 6915498 11.7 18010 -<br />
7147 RB12827 2.06699605 122 369 conserved hypothetical protein, membrane 6919729 6919361 12.5 13367 -<br />
7148 RB12829 2.28308508 159 480 conserved hypothetical protein 6919813 6920292 9.5 17677 +<br />
7152 RB12836 2.071792125 38 117 conserved hypothetical protein 6921619 6921735 10.8 4178 +<br />
7161 RB12851 1.900824009 109 330 conserved hypothetical protein 6926556 6926885 7.5 12033 +<br />
7167 RB12860 1.592005277 411 1236 Oligopeptide transport system permease protein oppC oppC 6932003 6930768 9.3 45876 -<br />
7170 RB12863 2.070343076 50 153 conserved hypothetical protein 6934850 6935002 12.6 5550 +<br />
7184 RB12891 1.69510928 258 777 LmbE-like protein 6944411 6945187 4.8 28480 +<br />
7186 RB12895 2.394774712 153 462 conserved hypothetical protein 6945636 6946097 10.7 17703 +<br />
7211 RB12959 1.765394992 107 324 conserved hypothetical protein 6974205 6973882 11.4 12121 -<br />
7221 RB12978 1.519476268 221 666 conserved hypothetical protein 6980722 6980057 4.9 23400 -<br />
7243 RB13025 2.580278072 193 582 monooxygenase 6997019 6996438 9.7 21953 -<br />
7251 RB13038 2.214420043 753 2262 conserved hypothetical protein, membrane 7005061 7002800 6.9 84132 -<br />
7273 RB13088 1.93093716 210 633 conserved hypothetical protein 7022309 7021677 5.6 24052 -<br />
7307 RB13139 2.205283961 416 1251 xylose operon regulatory protein xylR 7049875 7048625 9.5 46427 -<br />
7324 RB13172 1.940192944 78 237 conserved hypothetical protein 7070198 7069962 10.2 8753 -<br />
7330 RB13184 1.558333715 207 624 protein disulphide isomerase 7079314 7078691 6.2 23072 -<br />
7338 RB13198 1.638651618 124 375 conserved hypothetical protein 7084995 7085369 11.8 13938 +<br />
7348 RB13217 1.632026935 127 384 Penicillinase repressor blaI 7091881 7091498 9.3 14071 -<br />
707 RB1322 3.633490801 35 108 hypothetical protein 671526 671633 12.8 3914 +<br />
7352 RB13224 1.638640446 485 1458 nucleoside-diphosphate-sugar epimerase 7096270 7094813 9.5 53929 -<br />
7354 RB13228 1.822963597 116 351 conserved hypothetical protein 7097952 7098302 10.4 13570 +<br />
7363 RB13244 2.110430508 89 270 conserved hypothetical protein 7103330 7103061 10.3 9849 -<br />
7370 RB13259 1.966297351 634 1905 conserved hypothetical protein 7108365 7106461 5.7 72709 -<br />
7376 RB13267 1.800702079 239 720 conserved hypothetical protein 7117297 7116578 10.7 24985 -<br />
7400 RB13323 1.547636022 45 138 conserved hypothetical protein 7141536 7141673 11.7 5169 +<br />
715 RB1336 2.117833351 220 663 hypothetical protein 679357 680019 5.4 24662 +<br />
717 RB1339 1.700307676 101 306 hypothetical protein 681689 681384 11.1 11329 -<br />
725 RB1352 1.534628777 446 1341 hypothetical protein 686463 687803 5.5 46849 +<br />
733 RB1362 3.653375571 82 249 hypothetical protein 696352 696600 10.4 9054 +<br />
741 RB1384 1.983097668 177 534 membrane protein 707461 706928 9.8 19787 -<br />
759 RB1420 1.635284548 350 1053 inosine-uridine preferring nucleoside hydrolase 723375 724427 4.7 38646 +<br />
765 RB1432 1.713195711 199 600 NADH-ubiquinone oxidoreductase, chain 4L 1.6.5.3 732414 731815 4.2 21323 -<br />
766 RB1433 1.984728044 145 438 Na(+):H(+) antiporter subunit B (Multiple resistance and pH homeostasis protein B) mrpB 732848 732411 9.2 15860 -<br />
82 RB144 1.705177287 467 1404 conserved hypothetical protein 68273 66870 6.4 51000 -<br />
783 RB1463 1.653889741 100 303 hypothetical protein 743584 743886 9.6 10972 +<br />
799 RB1491 2.793289876 111 336 hypothetical protein 766600 766265 12.3 12531 -<br />
807 RB1507 2.441120945 334 1005 tRNA isopentenyltransferase 2.5.1.8 772202 771198 5.5 37063 -<br />
809 RB1509 2.837151983 156 471 hypothetical protein 773379 772909 6.2 17244 -<br />
810 RB1511 2.137167157 539 1620 hypothetical protein 774995 773376 5.2 59301 -<br />
811 RB1513 1.660911513 98 297 secreted protein 775203 774907 11.6 10491 -<br />
816 RB1519 1.508592061 74 225 hypothetical protein 777299 777075 11.3 8617 -<br />
823 RB1531 1.772778159 451 1356 membrane protein 782534 783889 4.5 50616 +<br />
839 RB1563 4.115142655 136 411 hypothetical protein 798934 799344 10.2 14882 +
840 RB1565 2.454683213 76 231 hypothetical protein 799552 799322 10.4 8648 -<br />
859 RB1601 2.37761474 75 228 hypothetical protein 824863 824636 10 8335 -<br />
861 RB1603 2.226007927 77 234 hypothetical protein 825177 824944 11.7 8721 -<br />
862 RB1604 1.654313812 399 1200 DNA alkylation repair enzyme 826373 825174 10.1 45416 -<br />
870 RB1621 2.277332839 350 1053 Phosphoesterase, PA-phosphatase related 835361 834309 6.5 38846 -<br />
871 RB1624 1.756863483 97 294 hypothetical protein 835745 835452 10.2 10697 -<br />
880 RB1638 1.825309659 65 198 hypothetical protein 842521 842718 6.1 6959 +<br />
94 RB164 1.995785075 166 501 hypothetical protein 87060 87560 9.8 19615 +<br />
885 RB1648 1.904541392 101 306 secreted protein 846423 846118 11.1 10776 -<br />
10 RB17 1.857901865 164 495 protein containing DUF1589 8251 7757 11.5 18498 -<br />
911 RB1717 1.687655588 541 1626 conserved hypothetical protein, membrane 897501 895876 10.3 60139 -<br />
927 RB1750 1.745721227 58 177 hypothetical protein 909661 909837 10.1 6339 +<br />
928 RB1751 1.940030528 68 207 hypothetical protein 910027 909821 10.5 7181 -<br />
937 RB1771 1.872017804 83 252 hypothetical protein 919524 919273 7.6 9390 -<br />
941 RB1778 3.146980452 69 210 hypothetical protein 921245 921036 10.7 7677 -<br />
963 RB1830 3.073407605 37 114 hypothetical protein 945611 945498 6.6 3673 -<br />
980 RB1860 1.704480171 110 333 hypothetical protein 960361 960693 10.9 11827 +<br />
981 RB1862 1.745419592 135 408 transcriptional regulator, GntR family 960630 961037 10.2 14884 +<br />
983 RB1868 1.995003137 835 2508 membrane protein 962128 964635 5.4 93195 +<br />
997 RB1893 1.700274014 1090 3273 Serine/threonine-protein kinase spk1 2.7.1.37 979639 982911 4.7 120745 +<br />
999 RB1896 2.940217947 106 321 membrane protein containing DUF1294 985295 985615 11.2 11976 +<br />
1003 RB1901 1.929260348 134 405 hypothetical protein 988429 988833 10.5 14815 +<br />
1008 RB1910 4.066965296 123 372 hypothetical protein 993764 993393 11.2 13384 -<br />
1053 RB2011 2.329166223 93 282 hypothetical protein 1059411 1059692 4.4 9880 +<br />
1068 RB2038 1.837125761 1553 4662 hypothetical protein 1076265 1071604 6.9 172935 -<br />
1069 RB2043 2.364366155 61 186 hypothetical protein 1076233 1076418 10.7 6909 +<br />
1122 RB2140 1.713943258 49 150 hypothetical protein 1131458 1131607 11.9 5498 +<br />
1126 RB2144 1.568531299 317 954 g eranylgeranyl pyrophosphate synthetase 1136089 1135136 5 33509 -<br />
1133 RB2155 1.989149473 156 471 hypothetical protein 1140660 1140190 4.9 17026 -<br />
1142 RB2173 2.986620064 354 1065 hypothetical protein 1147959 1146895 6.3 37862 -<br />
1150 RB2185 2.599831227 50 153 hypothetical protein 1152681 1152529 10.1 5841 -<br />
1151 RB2186 2.366402589 433 1302 ISXo8 transposase 1153993 1152692 9.4 49646 -<br />
1185 RB2252 1.718849778 53 162 hypothetical protein 1181701 1181540 9.8 6301 -<br />
1187 RB2254 1.811503778 541 1626 choline sulfatase betC 3.1.6.6 1184068 1182443 7.6 60782 -<br />
1193 RB2262 1.883608885 62 189 hypothetical protein 1188965 1188777 10.6 7138 -<br />
129 RB227 1.542512543 59 180 hypothetical protein 120858 121037 11.3 6741 +<br />
1209 RB2287 1.753647245 63 192 protein containing DUF1586 1199020 1198829 9.3 6890 -<br />
1223 RB2310 2.050664594 107 324 hypothetical protein 1209545 1209222 11.5 11581 -<br />
1235 RB2330 2.654605928 183 552 secreted protein 1214565 1214014 8.7 20019 -<br />
1245 RB2342 1.572016185 72 219 hypothetical protein 1217901 1218119 11.6 7727 +<br />
1275 RB2382 1.798186499 75 228 hypothetical protein 1238921 1239148 9.7 8092 +<br />
1283 RB2398 1.990644567 294 885 conserved hypothetical protein 1248404 1247520 4.7 31364 -<br />
1286 RB2401 2.51191176 706 2121 Phosphoesterase, PA-phosphatase related 1248679 1250799 3.7 76509 +<br />
1313 RB2457 2.235239536 118 357 hypothetical protein 1279788 1280144 12.7 13138 +<br />
1330 RB2477 1.792540082 123 372 hypothetical protein 1295793 1295422 10.2 13737 -<br />
1331 RB2478 2.716028379 38 117 hypothetical protein 1295756 1295872 10.7 4233 +<br />
1341 RB2492 3.097118649 69 210 hypothetical protein 1302190 1302399 9.9 7373 +<br />
1347 RB2498 1.838717804 296 891 methyltransferase, 1305777 1304887 6.2 33105 -<br />
1355 RB2507 2.535623852 392 1179 dTDP-glucose 4,6-dehydratase rfbB 4.2.1.46 1313064 1311886 5.2 42907 -<br />
1357 RB2509 1.680878147 46 141 hypothetical protein 1313237 1313377 5.4 4895 +<br />
1358 RB2510 1.686900431 55 168 hypothetical protein 1313396 1313563 10.7 5612 +<br />
1359 RB2511 2.147696849 45 138 hypothetical protein 1313530 1313667 11.8 5256 +<br />
1361 RB2513 1.567537981 46 141 hypothetical protein 1314624 1314764 4.7 4748 +<br />
1364 RB2516 2.301111387 63 192 hypothetical protein 1316669 1316860 11.2 6506 +<br />
1370 RB2524 2.265273084 115 348 hypothetical protein 1319449 1319796 7.4 11654 +<br />
1376 RB2534 2.03212221 100 303 hypothetical protein 1324897 1325199 11.8 11196 +<br />
1380 RB2545 3.271000229 52 159 hypothetical protein 1328931 1328773 11.2 5992 -<br />
1387 RB2556 2.296831119 60 183 Planctomycete PGAMP 1335620 1335438 13.1 6572 -<br />
1396 RB2574 1.570776807 249 750 hypothetical protein 1342282 1341533 10.8 26506 -<br />
1401 RB2585 2.259234668 89 270 Bacterial microcompartments protein 1346035 1345766 4.6 8850 -<br />
1402 RB2586 1.972115656 98 297 Bacterial microcompartments protein 1346402 1346106 9 9952 -<br />
1423 RB2627 1.611677151 342 1029 Glyceraldehyde 3-phosphate dehydrogenase gap 1.2.1.12 1364551 1365579 5.4 36716 +<br />
1428 RB2636 2.493759853 116 351 hypothetical protein 1368450 1368100 11.7 12761 -<br />
1431 RB2640 1.945623977 349 1050 Sugar phosphate isomerase/epimerase 1372769 1371720 4.6 38081 -<br />
1452 RB2679 1.995932725 173 522 hypothetical protein 1391557 1392078 5.5 19502 +<br />
1486 RB2740 1.59555329 126 381 hypothetical protein 1430028 1429648 10.5 13639 -<br />
1492 RB2747 2.234430805 80 243 hypothetical protein 1432443 1432685 10.1 8485 +<br />
1493 RB2748 1.778516188 387 1164 conserved hypothetical protein 1433804 1432641 5.8 44722 -<br />
1494 RB2749 1.673075235 305 918 4-hydroxybenzoate octaprenyltransferase ubiA 2.5.1.- 1434783 1433866 9.8 32877 -<br />
1495 RB2750 3.030088826 222 669 3-octaprenyl-4-hydroxybenzoate carboxy-lyase ubiX 4.1.1.- 1435465 1434797 6.3 24338 -<br />
1500 RB2764 2.655704279 841 2526 secreted protein containing DUF1585 1442155 1439630 5 94741 -<br />
1506 RB2784 2.156914688 203 612 membrane protein containing DUF107 1448065 1447454 4.5 21076 -<br />
155 RB279 2.278596176 308 927 ABC-type multidrug transport system, ATPase component 138584 139510 5 34067 +<br />
1511 RB2795 1.525548971 214 645 hypothetical protein 1454726 1455370 10.5 24354 +<br />
1522 RB2818 1.85753569 54 165 hypothetical protein 1463057 1462893 9.6 5981 -<br />
163 RB286 1.869403058 105 318 hypothetical protein 142779 143096 11.6 12056 +<br />
1553 RB2875 1.57863758 73 222 hypothetical protein 1497313 1497534 9.9 8090 +<br />
1555 RB2878 1.501456248 219 660 conserved hypothetical protein 1497670 1498329 6.1 24857 +<br />
1572 RB2912 1.974925237 313 942 secreted protein 1512238 1511297 4.4 33483 -<br />
1578 RB2924 1.531150096 143 432 secreted protein 1515456 1515025 8.9 16141 -<br />
1591 RB2943 2.015262346 86 261 hypothetical protein 1521275 1521535 9.5 9549 +<br />
1614 RB2976 1.647844896 196 591 ATP:cob(I)alamin adenosyltransferase 1536008 1535418 4.9 21602 -<br />
1619 RB2983 2.05395807 293 882 Hydrogen peroxide-inducible genes activator oxyR 1540680 1539799 6 33942 -<br />
1620 RB2985 2.358237497 365 1098 glycosyl transferase, group 1 family protein 1542040 1540943 5.1 40609 -<br />
1637 RB3013 2.141860925 77 234 hypothetical protein 1563386 1563619 12.3 7957 +<br />
1672 RB3073 2.369863432 51 156 hypothetical protein 1585180 1585025 9.1 5384 -<br />
179 RB314 2.187367848 309 930 malonyl CoA-acyl carrier protein transacylase fabD 2.3.1.39 156968 157897 4.4 32349 +<br />
1695 RB3140 1.675840507 111 336 hypothetical protein 1620838 1621173 10.2 12286 +<br />
1698 RB3144 1.582245667 348 1047 INTEGRASE/RECOMBINASE 1621739 1622785 9.6 39332 +<br />
1700 RB3146 2.738232396 98 297 protein containing DUF1556 1623952 1624248 11.4 10926 +<br />
1705 RB3152 2.029559749 68 207 hypothetical protein 1626667 1626461 12.2 7261 -<br />
1706 RB3153 2.297179262 76 231 hypothetical protein (protein containing DUF1584) 1626911 1626681 12.4 8266 -<br />
1708 RB3155 1.737756771 93 282 hypothetical protein 1627430 1627149 12.7 10369 -<br />
180 RB316 2.357048696 252 759 3-oxoacyl-[acyl-carrier-protein] reductase fabG 1.1.1.100 158031 158789 8.7 26160 +<br />
1722 RB3189 2.063916051 102 309 hypothetical protein 1646085 1646393 11.1 11570 +<br />
1742 RB3224 1.97493902 148 447 hypothetical protein 1656004 1656450 12.4 16377 +<br />
186 RB324 2.065626064 49 150 hypothetical protein 162731 162880 10.5 5404 +<br />
1755 RB3247 1.855081086 91 276 hypothetical protein 1668665 1668390 9.8 10018 -<br />
1766 RB3261 1.779391508 94 285 hypothetical protein 1678001 1677717 11.6 10565 -<br />
1803 RB3326 1.954199744 95 288 hypothetical protein 1711353 1711640 10.7 10480 +<br />
1809 RB3333 2.026225922 39 120 hypothetical protein 1713409 1713528 12.2 4095 +<br />
191 RB335 2.057101688 85 258 hypothetical protein 168642 168385 12.6 9143 -<br />
195 RB341 2.265892107 90 273 hypothetical protein 169852 170124 11.2 10096 +<br />
1878 RB3449 1.601850015 52 159 hypothetical protein 1782475 1782317 11.3 5845 -<br />
1915 RB3509 1.521458235 336 1011 glycosyl hydrolase 1818015 1819025 5 37024 +<br />
1942 RB3565 1.920719145 137 414 membrane protein 1853670 1854083 10.1 15653 +<br />
1964 RB3599 2.279590265 365 1098 KLHL5 protein 1871888 1872985 6.5 40419 +<br />
1987 RB3633 1.666390417 90 273 hypothetical protein 1890483 1890211 12 10096 -<br />
2005 RB3659 2.633863645 59 180 hypothetical protein 1900428 1900607 10.7 6782 +
2008 RB3662 2.034633667 43 132 hypothetical protein 1902024 1901893 10.6 4816 -<br />
2009 RB3663 1.866613594 69 210 secreted protein 1902328 1902119 12.1 7609 -<br />
2129 RB3864 2.159302365 130 393 hypothetical protein 1998657 1999049 12.2 14855 +<br />
2130 RB3865 1.657844263 178 537 acetyltransferase, GNAT family 1999065 1999601 6.1 20057 +<br />
2295 RB4160 2.226402394 386 1161 conserved hypothetical protein 2136438 2137598 5.1 43127 +<br />
2300 RB4167 1.933027443 979 2940 secreted cytochrome c-like protein 2144846 2141907 4.8 107275 -<br />
2308 RB4185 2.383681463 89 270 hypothetical protein 2151951 2152220 12 9730 +<br />
2312 RB4194 2.433488216 53 162 hypothetical protein 2156685 2156524 11.4 5728 -<br />
2388 RB4306 1.625468979 457 1374 transposase and inactivated derivative 2221207 2219834 8.4 52492 -<br />
2398 RB4325 3.765600993 48 147 Planctomycete PGAMP 2228477 2228623 12.5 5403 +<br />
2403 RB4338 2.78496096 57 174 hypothetical protein 2233809 2233636 6.3 6607 -<br />
2405 RB4341 1.659604036 109 330 hypothetical protein 2234849 2235178 7.5 12018 +<br />
2440 RB4397 2.691758559 55 168 protein containing DUF1560 2263976 2264143 10.5 5816 +<br />
2454 RB4420 1.767751346 206 621 DedA family protein 2274093 2273473 9.3 22775 -<br />
2457 RB4425 1.812016826 127 384 hypothetical protein 2275100 2275483 10.6 14111 +<br />
2466 RB4440 1.634912345 347 1044 Amidohydrolase 2 4.1.1.45 2278882 2279925 6.6 39008 +<br />
2475 RB4457 2.385063444 98 297 hypothetical protein 2285628 2285924 10 10915 +<br />
2487 RB4473 1.514698444 57 174 hypothetical protein 2294981 2294808 9.1 5884 -<br />
2493 RB4482 2.526830138 111 336 hypothetical protein 2297240 2296905 12 12929 -<br />
2501 RB4498 2.197458016 90 273 hypothetical protein 2303522 2303250 9.4 9942 -<br />
2509 RB4510 3.283537625 49 150 hypothetical protein 2307657 2307508 9 5554 -<br />
2521 RB4530 3.168417353 1130 3393 heavy metal efflux pump, CzcA family 2323867 2320475 4.8 123379 -<br />
2542 RB4572 2.349259751 90 273 hypothetical protein 2339375 2339103 7.7 9938 -<br />
2558 RB4598 1.584206651 306 921 membrane protein 2355806 2356726 4 33125 +<br />
2559 RB4601 2.100239027 318 957 protein containing DUF1559 2356778 2357734 5.3 34567 +<br />
2587 RB4645 1.641416077 69 210 hypothetical protein 2378266 2378057 10.9 7946 -<br />
2593 RB4652 1.897678047 52 159 hypothetical protein 2380494 2380652 12.6 5628 +<br />
2601 RB4662 3.347879694 37 114 secreted protein 2383563 2383676 4.4 4069 +<br />
2613 RB4691 2.507103018 284 855 hypothetical protein 2395001 2395855 12 32256 +<br />
2622 RB4705 1.622136032 56 171 hypothetical protein 2403864 2403694 12.6 6330 -<br />
2637 RB4727 2.004479953 54 165 hypothetical protein 2422602 2422766 9.1 5908 +<br />
2654 RB4750 1.53101482 204 615 membrane protein 2435462 2436076 10 23312 +<br />
2673 RB4781 2.200497845 156 471 HesB/YadR/YfhF 2449655 2450125 4.6 17413 +<br />
2699 RB4815 1.865895895 491 1476 arylsulfatase precursor ARSA 3.1.6.8 2469289 2467814 5.7 54617 -<br />
2704 RB4826 1.656393726 464 1395 Transposase IS66 2471205 2472599 10.2 51739 +<br />
282 RB483 2.063475736 54 165 hypothetical protein 262569 262733 12.2 6454 +<br />
2717 RB4850 1.857021056 150 453 membrane protein 2484018 2484470 7.6 15931 +<br />
2745 RB4894 1.639002853 712 2139 glycogen debranching enzyme glgX 3.2.1.- 2518939 2516801 5 81197 -<br />
2783 RB4957 2.146895728 57 174 hypothetical protein 2548380 2548553 12.4 6569 +<br />
2792 RB4969 2.023603795 334 1005 hypothetical protein 2554443 2555447 10 37084 +<br />
2821 RB5010 2.70359212 94 285 hypothetical protein 2577161 2576877 11.9 10778 -<br />
2830 RB5029 2.463803039 236 711 conserved hypothetical protein, secreted 2589744 2589034 4.6 26291 -<br />
2867 RB5093 1.893127073 67 204 hypothetical protein 2619010 2619213 6.3 7246 +<br />
2894 RB5138 2.710550482 58 177 hypothetical protein 2641024 2640848 12.4 6711 -<br />
2908 RB5164 2.562994028 218 657 hypothetical protein 2658365 2657709 11.4 24275 -<br />
2910 RB5168 1.794347644 60 183 hypothetical protein 2658508 2658690 9.7 6626 +<br />
299 RB519 2.066265663 54 165 hypothetical protein 285036 284872 10.6 6171 -<br />
2926 RB5195 1.527558092 505 1518 arylsulfatase Arsb 3.1.6.12 2674328 2675845 5 56413 +<br />
301 RB521 4.512236615 63 192 hypothetical protein 286399 286208 10.7 7040 -<br />
2984 RB5294 1.540465479 533 1602 sulfatase 2732584 2730983 5.6 61291 -<br />
3023 RB5371 1.76572172 109 330 hypothetical protein 2774829 2775158 7.5 12033 +<br />
3047 RB5412 3.524059925 72 219 hypothetical protein 2794838 2794620 12.3 8592 -<br />
316 RB546 3.008885687 485 1458 secreted protein containing DUF1501 302834 304291 7.8 53385 +<br />
3103 RB5502 2.242859912 144 435 membrane protein 2845859 2846293 3.1 15161 +<br />
321 RB556 1.708513615 47 144 hypothetical protein 308213 308356 12.4 5508 +<br />
322 RB557 1.801550469 53 162 hypothetical protein 308223 308384 10.5 5688 +<br />
3142 RB5572 1.937860486 39 120 hypothetical protein 2901618 2901499 13 4731 -<br />
3153 RB5597 2.927301342 170 513 conserved hypothetical protein, secreted 2913295 2913807 11.9 17556 +<br />
324 RB560 2.240012693 280 843 protein containing DGPFAETKE domain DGPF 309384 308542 4.9 30721 -<br />
3215 RB5707 3.494468009 183 552 hypothetical protein 2979435 2979986 10.2 20474 +<br />
3233 RB5737 2.165159487 120 363 hypothetical protein 2994415 2994777 11.9 13608 +<br />
3292 RB5846 1.552211655 297 894 glycosyltransferase 3064164 3065057 6.3 32718 +<br />
3304 RB5867 1.636075198 50 153 hypothetical protein 3077578 3077730 9.7 5871 +<br />
3309 RB5874 1.69375552 47 144 hypothetical protein 3080941 3080798 4.6 5338 -<br />
3312 RB5877 1.674709272 35 108 hypothetical protein 3082302 3082409 10.6 3895 +<br />
3319 RB5887 2.051189207 292 879 integrase 3087343 3086465 9.9 33335 -<br />
3334 RB5914 2.906505812 56 171 hypothetical protein 3097264 3097434 10.5 6574 +<br />
3345 RB5937 2.853944961 306 921 hypothetical protein 3112147 3113067 11.7 34734 +<br />
3361 RB5966 1.580545054 140 423 hypothetical protein 3128115 3128537 10.1 15593 +<br />
3374 RB5988 2.208521161 174 525 secreted protein 3141494 3140970 9.4 18622 -<br />
3376 RB5990 2.310006948 113 342 hypothetical protein 3141918 3141577 11.8 12587 -<br />
3390 RB6011 1.682557663 50 153 hypothetical protein 3150235 3150083 10.1 5841 -<br />
3394 RB6016 1.906776176 188 567 hypothetical protein 3152860 3153426 11.7 20633 +<br />
3426 RB6081 1.51616219 52 159 hypothetical protein 3191035 3190877 11.5 5837 -<br />
3440 RB6108 1.523955685 1096 3291 secreted protein 3208185 3204895 5.4 123062 -<br />
3459 RB6147 1.516334798 394 1185 3-dehydroquinate synthase aroB 4.2.3.4 3229258 3230442 6.2 43267 +<br />
3471 RB6167 1.500109148 375 1128 Putative transposase 3235937 3237064 9.9 41895 +<br />
3472 RB6168 2.304188414 208 627 hypothetical protein 3236689 3237315 12.2 22575 +<br />
3477 RB6175 2.184505189 125 378 hypothetical protein 3240125 3239748 11.9 14247 -<br />
3482 RB6188 3.009885374 68 207 hypothetical protein 3244384 3244178 9.6 6986 -<br />
3513 RB6245 1.931203785 107 324 hypothetical protein 3278762 3279085 12.4 12397 +<br />
3516 RB6250 1.514075438 35 108 hypothetical protein 3282079 3282186 9.4 4025 +<br />
3520 RB6257 3.142660037 207 624 secreted protein 3288666 3288043 4.6 22458 -<br />
3573 RB6355 1.53314894 505 1518 iron-sulfur cluster-binding protein 3336973 3338490 9.6 55993 +<br />
3584 RB6374 2.969521508 442 1329 conserved hypothetical protein 3348288 3349616 9 48323 +<br />
3585 RB6375 2.716117152 297 894 hypothetical protein 3349681 3350574 8.7 31772 +<br />
3589 RB6380 2.144492471 74 225 hypothetical protein 3353359 3353583 12.4 8433 +<br />
3591 RB6382 2.35405938 39 120 hypothetical protein 3355162 3355043 10.4 4208 -<br />
3598 RB6390 1.942322628 86 261 hypothetical protein 3361959 3362219 12.8 9768 +<br />
3607 RB6405 1.914661933 93 282 hypothetical protein 3371368 3371087 9.6 10148 -<br />
3629 RB6440 1.624957167 57 174 hypothetical protein 3391656 3391829 10.4 6422 +<br />
3642 RB6459 1.590981738 2028 6087 cell surface protein 3408788 3414874 3.5 214858 +<br />
3711 RB6571 1.630308658 154 465 secreted protein 3489550 3490014 9 17657 +<br />
39 RB66 1.62209628 84 255 hypothetical protein 26082 26336 11.1 9333 +<br />
3735 RB6619 1.663215867 262 789 hypothetical protein 3518291 3517503 9.7 29227 -<br />
362 RB663 2.084810827 79 240 hypothetical protein 357608 357369 11.4 8792 -<br />
3753 RB6655 1.582402313 36 111 hypothetical protein 3535829 3535719 9.4 4005 -<br />
3756 RB6661 1.538114086 88 267 hypothetical protein 3538752 3538486 10.6 9093 -<br />
3770 RB6684 1.61337283 105 318 hypothetical protein 3554034 3553717 11.6 11612 -<br />
3771 RB6685 1.596892111 72 219 hypothetical protein 3554265 3554047 12.2 8077 -<br />
365 RB670 1.649686669 136 411 hypothetical protein 359315 358905 10.1 15444 -<br />
3784 RB6706 1.931579477 37 114 hypothetical protein 3560942 3560829 7.3 4375 -<br />
366 RB671 2.477782668 122 369 hypothetical protein 359218 359586 10.7 13383 +<br />
3793 RB6720 2.082468911 72 219 hypothetical protein 3567752 3567970 10.3 8140 +<br />
3804 RB6736 2.854676533 433 1302 ISXo8 transposase 3578852 3577551 9.4 49646 -<br />
3817 RB6761 1.720050338 198 597 bacterioferritin comigratory protein 3594163 3594759 5.7 21350 +<br />
3834 RB6786 1.685047733 172 519 hypothetical protein 3607257 3607775 12.3 20026 +<br />
3852 RB6822 2.882630068 404 1215 Chorismate synthase aroC 4.2.3.5 3623410 3624624 5.8 42918 +<br />
3885 RB6883 2.308615096 255 768 Lipoprotein releasing system ATP-binding protein lolD lolD 3657855 3658622 5.6 28302 +
3893 RB6903 1.641538975 53 162 hypothetical protein 3669220 3669059 9.3 6058 -<br />
3904 RB6916 2.497799815 67 204 hypothetical protein 3676188 3676391 12 7266 +<br />
3909 RB6925 3.784134297 161 486 hypothetical protein 3678461 3677976 9.8 17641 -<br />
3911 RB6930 3.097264296 413 1242 glutamate dehydrogenase 1.4.1.3 3680317 3679076 5.9 45734 -<br />
3912 RB6932 2.972180516 319 960 CYSTEINE SYNTHASE A cysK 2.5.1.47 3681308 3680349 5 33739 -<br />
3914 RB6935 1.797951989 627 1884 pyruvate:ferredoxin oxidoreductase (alpha subunit) 3684260 3682377 5.5 67263 -<br />
3940 RB6989 2.291433415 82 249 hypothetical protein 3719206 3718958 12.5 9143 -<br />
3949 RB7002 1.955400985 232 699 secreted protein 3725925 3726623 7.7 25003 +<br />
3956 RB7016 2.59004597 241 726 hypothetical protein 3732513 3733238 7.7 26263 +<br />
3963 RB7028 1.604036296 545 1638 hypothetical protein 3736857 3738494 5.1 58222 +<br />
4013 RB7114 1.673446914 347 1044 phenylalanyl-tRNA synthetase alpha chain pheS 6.1.1.20 3779252 3778209 4.6 38018 -<br />
4015 RB7116 3.58641694 59 180 hypothetical protein 3779586 3779765 11.7 6458 +<br />
4027 RB7136 1.638258201 68 207 hypothetical protein 3787932 3788138 7.4 7499 +<br />
4047 RB7176 1.561644294 46 141 hypothetical protein 3806783 3806643 10.1 4647 -<br />
392 RB723 2.617929723 60 183 hypothetical protein 388051 388233 7.3 6624 +<br />
4118 RB7292 2.345087554 256 771 metallo-beta-lactamase 3875577 3876347 5.4 28641 +<br />
4135 RB7318 3.003763502 126 381 hypothetical protein 3895264 3894884 7.3 13046 -<br />
4136 RB7319 2.55122604 76 231 hypothetical protein 3895491 3895261 12.4 8291 -<br />
4156 RB7388 1.795157437 282 849 INTEGRASE/RECOMBINASE 3952745 3953593 10.6 31873 +<br />
4157 RB7389 2.206131059 375 1128 Putative transposase 3953590 3954717 9.9 41895 +<br />
4158 RB7390 1.5717743 208 627 hypothetical protein 3954342 3954968 12.2 22575 +<br />
4160 RB7393 1.768724913 66 201 hypothetical protein 3955353 3955553 11 7563 +<br />
4203 RB7464 3.263712295 44 135 hypothetical protein 4001614 4001480 10.2 4711 -<br />
4207 RB7471 1.769148263 92 279 hypothetical protein 4006361 4006083 11.9 10550 -<br />
4216 RB7486 1.83812896 130 393 hypothetical protein 4017064 4016672 8.8 14588 -<br />
403 RB754 2.092237647 364 1095 Polysaccharide export protein 403211 404305 4.2 39246 +<br />
4285 RB7599 1.837320006 39 120 hypothetical protein 4086498 4086379 10.8 4566 -<br />
4318 RB7651 1.910539418 44 135 hypothetical protein 4112517 4112383 6.7 4749 -<br />
4319 RB7652 2.842117135 315 948 malate dehydrogenase mdh 1.1.1.37 4112505 4113452 4.5 33055 +<br />
4332 RB7678 1.89392125 226 681 protocatechuate 3,4-dioxygenase type II beta subunit 4123339 4122659 9.4 25090 -<br />
4345 RB7703 2.098931056 98 297 hypothetical protein 4131842 4132138 8.9 10578 +<br />
4400 RB7790 1.879833001 89 270 hypothetical protein 4173233 4173502 10.6 9770 +<br />
4425 RB7833 1.690086332 228 687 Ribosomal protein L3 4192692 4193378 11 24681 +<br />
4449 RB7875 2.32438741 492 1479 Arylsulfatase A precursor ARSA 3.1.6.8 4208176 4209654 5.4 54661 +<br />
4470 RB7909 1.671996189 330 993 secreted protein 4225494 4224502 10.6 34211 -<br />
4478 RB7924 1.688366017 228 687 RNA polymerase sigma factor 4231813 4232499 6.2 25242 +<br />
4548 RB8065 1.999674433 38 117 hypothetical protein 4318540 4318424 10.4 3952 -<br />
4561 RB8084 2.15890448 38 117 hypothetical protein 4330006 4329890 12.4 4461 -<br />
4573 RB8107 1.84185736 315 948 secreted protein 4339329 4340276 5.4 34412 +<br />
4582 RB8127 1.688135503 262 789 hypothetical protein 4349191 4349979 11.7 28414 +<br />
4586 RB8138 1.99933795 75 228 hypothetical protein 4351960 4352187 4.1 8440 +<br />
4632 RB8231 1.523420874 170 513 ACT domain protein 4401779 4402291 4.4 18932 +<br />
4643 RB8248 1.579525345 253 762 dienelactone hydrolase family protein 4409395 4410156 5.7 28139 +<br />
4648 RB8260 1.55919706 108 327 protein containing DUF167 4415394 4415068 10.5 11641 -<br />
4655 RB8275 1.614651716 45 138 hypothetical protein 4424897 4424760 11.4 5115 -<br />
4657 RB8279 2.194205192 71 216 hypothetical protein 4425128 4425343 5.8 7835 +<br />
4696 RB8353 1.828073353 82 249 hypothetical protein 4459374 4459622 10.8 9402 +<br />
4699 RB8357 1.947454134 56 171 hypothetical protein 4460174 4460004 7.4 6097 -<br />
4714 RB8384 1.753384306 433 1302 ISXo8 transposase 4478623 4479924 9.4 49646 +<br />
457 RB839 2.182062009 60 183 hypothetical protein 441662 441844 6.1 6654 +<br />
4724 RB8404 1.711268941 239 720 Silent information regulator protein Sir2 4492899 4493618 4.7 26142 +<br />
4749 RB8457 3.160312784 113 342 hypothetical protein 4518458 4518117 11.5 12525 -<br />
4755 RB8468 1.777768949 88 267 hypothetical protein 4520799 4520533 11.9 9719 -<br />
4761 RB8478 1.742151616 141 426 hypothetical protein 4524759 4524334 11.6 15805 -<br />
4794 RB8530 1.749276032 53 162 hypothetical protein 4550886 4550725 12.3 5816 -<br />
4802 RB8541 1.642385639 357 1074 Peptidase M42 4554758 4555831 4.6 38279 +<br />
4861 RB8669 1.687927001 37 114 hypothetical protein 4614557 4614670 11.5 4395 +<br />
471 RB867 2.017027094 100 303 hypothetical protein 460731 460429 9.7 11273 -<br />
4862 RB8670 4.214509032 47 144 hypothetical protein 4614842 4614985 11.1 5371 +<br />
4879 RB8697 3.261367962 56 171 hypothetical protein 4630281 4630111 9.7 6374 -<br />
4880 RB8700 3.773767421 58 177 hypothetical protein 4630224 4630400 4.8 6268 +<br />
4891 RB8723 2.487879984 74 225 hypothetical protein 4638077 4638301 12 8520 +<br />
4893 RB8726 1.567912793 599 1800 single-stranded-DNA-specific exonuclease RecJ recJ 3.1.-.- 4638505 4640304 5.3 64794 +<br />
4905 RB8747 2.083737467 218 657 DNA/pantothenate metabolism flavoprotein 4648152 4648808 5.5 23702 +<br />
4906 RB8748 2.332252043 305 918 dihydroorotate dehydrogenase pyrD 1.3.3.1 4648815 4649732 4.6 31813 +<br />
4941 RB8815 3.22054004 71 216 hypothetical protein 4687955 4687740 4.8 7683 -<br />
4943 RB8818 2.134712008 60 183 hypothetical protein 4689021 4688839 10.4 6695 -<br />
4956 RB8840 2.085169881 94 285 acylphosphatases 4701445 4701729 10.2 10806 +<br />
4976 RB8876 1.625211468 87 264 hypothetical protein 4723629 4723892 9.5 9540 +<br />
4982 RB8884 2.25520575 70 213 hypothetical protein 4727666 4727878 11.8 8000 +<br />
4994 RB8902 2.296035668 82 249 hypothetical protein 4740474 4740226 11.2 9412 -<br />
5004 RB8917 2.592910345 74 225 hypothetical protein 4747435 4747659 10.4 8075 +<br />
5031 RB8975 2.603055071 110 333 hypothetical protein 4783888 4783556 10.4 12068 -<br />
5035 RB8980 1.741796604 618 1857 protein containing DUF1559 4788689 4786833 4.8 67763 -<br />
5036 RB8983 1.679549934 346 1041 methanol dehydrogenase regulatory protein 4788960 4790000 4.8 37993 +<br />
5038 RB8987 1.637584191 48 147 hypothetical protein 4790856 4790710 9.1 5309 -<br />
5050 RB9017 1.676962039 193 582 hypthetical protein 4805225 4805806 6.3 21847 +<br />
5055 RB9024 1.646716221 46 141 hypothetical protein 4809190 4809050 10.3 5125 -<br />
5061 RB9037 1.681174613 344 1035 secreted protein 4821537 4822571 6 36987 +<br />
5062 RB9038 2.739410555 153 462 hypothetical protein 4823103 4822642 10.5 17019 -<br />
5079 RB9078 1.55341567 969 2910 2-oxoglutarate dehydrogenase E1 component OGDH 1.2.4.2 4846881 4849790 5.7 109319 +<br />
5116 RB9144 1.996588404 150 453 hypothetical protein 4893346 4893798 11 16267 +<br />
496 RB919 1.772829794 109 330 hypothetical protein 488799 488470 10.2 11958 -<br />
5136 RB9190 2.009547343 132 399 ferredoxin 4914039 4914437 5 14357 +<br />
5140 RB9196 2.109875658 446 1341 secreted protein containing DUF1080 4916635 4917975 5.4 50522 +<br />
5142 RB9198 5.126650093 58 177 hypothetical protein 4918032 4918208 11.8 6612 +<br />
5158 RB9231 2.16301151 109 330 hypothetical protein 4937008 4936679 7.5 12033 -<br />
500 RB924 2.860471873 208 627 hypothetical protein 490400 489774 12.2 22548 -<br />
5169 RB9249 1.661379564 810 2433 hypothetical protein 4947571 4945139 5.4 86740 -<br />
5181 RB9276 1.574652752 233 702 membrane protein 4958461 4959162 7.5 24790 +<br />
5197 RB9308 2.017185123 629 1890 Outer membrane efflux family protein 4978849 4976960 6.9 69055 -<br />
504 RB931 2.874769085 39 120 hypothetical protein 492159 492278 9.4 4169 +<br />
506 RB933 2.254207171 208 627 hypothetical protein 493451 492825 12.2 22575 -<br />
5211 RB9338 1.506192607 182 549 formaldehyde-activating enzyme fae 5002305 5002853 5.6 19774 +<br />
507 RB934 2.171115834 375 1128 Putative transposase 494203 493076 9.9 41881 -<br />
5217 RB9346 1.777973228 71 216 hypothetical protein 5008335 5008550 10.5 7755 +<br />
508 RB936 1.589630607 85 258 hypothetical protein 494524 494267 11.1 9715 -<br />
5230 RB9365 3.002822981 152 459 hypothetical protein 5017351 5017809 11.6 16637 +<br />
5257 RB9415 2.013265204 36 111 hypothetical protein 5050419 5050529 10.6 4057 +<br />
5278 RB9450 1.892134357 52 159 hypothetical protein 5076581 5076739 7.3 5967 +<br />
522 RB957 1.975418743 195 588 Tyrosine recombinase xerD xerD 505476 504889 10.2 21971 -<br />
5355 RB9592 1.552934579 269 810 conserved hypothetical protein 5157928 5158737 5.5 30261 +<br />
5415 RB9702 2.664021836 96 291 conserved hypothetical protein 5229342 5229632 4.6 9322 +<br />
5461 RB9793 1.845305093 63 192 hypothetical protein 5273041 5272850 12.5 6957 -<br />
5524 RB9907 1.925591118 433 1302 ISXo8 transposase 5338185 5339486 9.4 49646 +<br />
5525 RB9908 2.150644277 50 153 hypothetical protein 5339497 5339649 9.9 5867 +<br />
5532 RB9918 2.745466406 167 504 hypothetical protein 5344369 5344872 5.3 18361 +
Cluster 3<br />
Original row UID Start Stop EC 62 h vs 44 h 82 h va 62 h 96 h vs 82 h 240 h vs 82 h<br />
156 RB10246_Triphosphoribosyl-dephospho-CoA protein (ORF7) 5538891 5539841 NaN 0 NaN 2.58<br />
192 RB10319_hypothetical protein 5577574 5577771 NaN 0 NaN 3.61<br />
692 RB11168_thiosulfate sulfurtransferase 6062177 6061728 0 0 NaN 2.88<br />
1101 RB11855_conserved hypothetical protein 6383765 6383460 0 0 NaN 2.2<br />
1360 RB12286_conserved hypothetical protein 6610293 6610120 NaN 0 0 1.7<br />
1453 RB12427_conserved hypothetical protein 6684645 6684475 NaN 0 NaN 2.18<br />
1533 RB12554_conserved hypothetical protein 6764163 6764378 NaN 0 0 2.45<br />
1727 RB12860_Oligopeptide transport system permease protein oppC 6932003 6930768 NaN 0 0 1.59<br />
2101 RB1509_hypothetical protein 773379 772909 0 0 0 2.84<br />
2108 RB1519_hypothetical protein 777299 777075 0 0 0 1.51<br />
2854 RB2750_3-octaprenyl-4-hydroxybenzoate carboxy-lyase 1435465 1434797 4.1.1.- 0 0 0 3.03<br />
3356 RB3565_membrane protein 1853670 1854083 0 0 0 1.92<br />
3923 RB4420_DedA family protein 2274093 2273473 NaN 0 0 1.77<br />
4217 RB4850_membrane protein 2484018 2484470 0 0 0 1.86<br />
4330 RB5010_hypothetical protein 2577161 2576877 0 0 0 2.7<br />
5163 RB6380_hypothetical protein 3353359 3353583 0 0 0 2.14<br />
5423 RB6786_hypothetical protein 3607257 3607775 0 0 0 1.69<br />
5980 RB7703_hypothetical protein 4131842 4132138 0 0 0 2.1<br />
6295 RB8231_ACT domain protein 4401779 4402291 0 0 0 1.52<br />
6579 RB8723_hypothetical protein 4638077 4638301 0 0 NaN 2.49<br />
6709 RB893_protein containing cohesin and planctomycete extracellular domains (cellulosome-like) 476445 477704 0 0 0 1.45<br />
6916 RB931_hypothetical protein 492159 492278 0 0 NaN 2.87<br />
6983 RB9415_hypothetical protein 5050419 5050529 0 0 0 2.01<br />
1 RB100_membrane protein 41097 40726 NaN 0 0 1.65<br />
8 RB10010_secreted protein containing DUF1585 5393982 5391460 NaN 0 NaN 4.37<br />
13 RB10020_hypothetical protein 5396788 5396594 NaN 0 NaN 2.19<br />
19 RB10026_conserved hypothetical protein 5401902 5399251 NaN 0 NaN 2.16<br />
58 RB10096_Transposase IS116/IS110/IS902 5446891 5445866 NaN 0 NaN 2.67<br />
155 RB10245_haloalkane dehalogenase 5537951 5538937 3.8.1.5 NaN 0 NaN 2.2<br />
158 RB10252_hypothetical protein 5541386 5542546 NaN 0 NaN 2.29<br />
159 RB10255_MscS Mechanosensitive ion channel 5542656 5543669 NaN 0 0 3.7<br />
160 RB10256_hypothetical protein 5543753 5544559 0 0 0 3.23<br />
161 RB10258_Survival protein SurE 5544584 5545453 3.1.3.2 0 0 0 3.22<br />
173 RB10277_Pyruvate kinase 5555414 5556844 2.7.1.40 NaN 0 NaN 2.9<br />
179 RB10290_hypothetical protein 5559631 5559203 NaN 0 NaN 2.93<br />
181 RB10295_methylene tetrahydrofolate/methylene tetrahydromethanopterin dehydrogenase-like protein 5562427 5561501 0 0 0 1.7<br />
187 RB10308_response regulator 5569131 5570057 NaN 0 0 1.55<br />
198 RB10328_protein containing DUF633 5583675 5582992 NaN 0 NaN 2.94<br />
205 RB10338_TPR domain protein 5591839 5590379 NaN 0 0 2.43<br />
336 RB10569_protein containing DUF1559 5736174 5735008 0 0 0 1.58<br />
361 RB10608_transposase and inactivated derivative 5759903 5761276 0 0 0 2.06<br />
404 RB10682_secreted protein 5807422 5805998 0 0 0 1.65<br />
409 RB10689_hypothetical protein 5808207 5807950 0 0 0 1.62
414 RB10698_hypothetical protein 5813025 5812573 NaN 0 0 1.84<br />
437 RB10735_hypothetical protein 5834722 5835720 0 0 0 1.98<br />
512 RB10866_hypothetical protein 5899291 5899043 0 0 0 3.29<br />
531 RB10893_hypothetical protein 5914894 5915067 0 0 0 2.85<br />
565 RB1095_hypothetical protein 567469 569046 0 0 0 1.81<br />
572 RB10960_conserved hypothetical protein, membrane 5941602 5942021 0 0 0 1.54<br />
577 RB1097_membrane protein 570281 569067 NaN 0 0 1.58<br />
599 RB11001_conserved hypothetical protein 5964931 5964047 0 0 0 3.1<br />
602 RB11009_sulfite reductase-like flavoprotein 5969752 5969036 NaN 0 NaN 3.75<br />
603 RB11010_conserved hypothetical protein, secreted 5970585 5969749 0 0 0 2.91<br />
604 RB11011_conserved hypothetical protein, secreted 5971127 5970582 NaN 0 NaN 2.96<br />
605 RB11013_conserved hypothetical protein, membrane 5971802 5971146 NaN 0 NaN 2.91<br />
617 RB11036_two-component system, regulatory protein 5985609 5984197 NaN 0 0 1.91<br />
618 RB11037_two-component system sensor protein 5987098 5985617 NaN 0 0 1.86<br />
644 RB11082_hypothetical protein 6007371 6007033 0 0 0 2.92<br />
735 RB11233_hypothetical protein 6090340 6089453 0 0 0 2.22<br />
771 RB11293_hypothetical protein 6121182 6120889 0 0 0 1.56<br />
810 RB11348_conserved hypothetical protein 6155692 6155237 0 0 NaN 2.31<br />
825 RB1138_conserved hypothetical protein 585492 585959 NaN 0 0 2.5<br />
872 RB11462_CoA-binding protein 6202949 6203338 0 0 0 2.29<br />
959 RB11617_conserved hypothetical protein 6263689 6262796 NaN 0 NaN 2.65<br />
977 RB11641_conserved hypothetical protein 6278514 6277621 0 0 NaN 2.15<br />
984 RB11650_NADH-dependent dehydrogenase 6284983 6283604 0 0 0 1.47<br />
1002 RB11677_conserved hypothetical protein 6300221 6300078 NaN 0 NaN 2.21<br />
1029 RB11720_conserved hypothetical protein 6316234 6317028 0 0 0 1.53<br />
1032 RB11728_D-Tagatose 3-epimerase 6318857 6319744 5.3.1.- NaN 0 0 1.86<br />
1033 RB11729_conserved hypothetical protein 6320009 6319752 NaN 0 0 2.1<br />
1058 RB11768_conserved hypothetical protein 6334808 6334939 0 0 0 2.49<br />
1061 RB1179_hypothetical protein 603998 604384 1.84 0 0 1.84<br />
1100 RB11854_conserved hypothetical protein 6383404 6383171 0 0 0 1.57<br />
1105 RB11862_conserved hypothetical protein, secreted 6387327 6387157 0 0 0 1.63<br />
1131 RB1191_hypothetical transposase 608435 608055 NaN 0 NaN 2.62<br />
1161 RB11951_conserved hypothetical protein 6437923 6438510 NaN 0 0 3.12<br />
1195 RB12006_regulatory subunit 6475429 6476454 NaN 0 0 1.72<br />
1198 RB1201_hypothetical protein 612754 612629 0 0 NaN 2.5<br />
1201 RB12015_conserved hypothetical protein 6483388 6482423 NaN 0 0 1.61<br />
1210 RB12031_conserved hypothetical protein 6490799 6490359 NaN 0 0 1.95<br />
1222 RB12053_biopolymer transport protein, ExbD/TolR family 6502558 6502199 0 0 -1.5 1.73<br />
1223 RB12055_MotA/TolQ/ExbB proton channel family protein 6503587 6502628 NaN 0 0 2.85<br />
1231 RB12068_serine/threonine protein kinase 6512255 6510594 1.63 0 0 2.04<br />
1239 RB12080_Protein-L-isoaspartate(D-aspartate) O-methyltransferase 6517231 6515753 2.1.1.77 NaN 0 0 1.62<br />
1249 RB1210_AP endonuclease 2, C-terminal 617416 616571 NaN 0 0 2.29<br />
1305 RB12200_conserved hypothetical protein 6577955 6577800 0 0 0 1.91<br />
1325 RB1223_hypothetical protein 624091 624312 NaN 0 NaN 2.35<br />
1332 RB12239_ISXo8 transposase 6593334 6594635 NaN 0 NaN 2.63
1339 RB1225_dipeptidyl peptidase IV 627017 624513 3.4.14.5 NaN 0 0 1.56<br />
1355 RB12279_MscS Mechanosensitive ion channel 6606558 6605545 NaN 0 0 1.85<br />
1366 RB12295_conserved hypothetical protein 6614190 6614450 NaN 0 0 1.55<br />
1420 RB12379_riboflavin synthase, alpha subunit 6662808 6662086 0 0 0 3.87<br />
1424 RB12383_conserved hypothetical protein 6664767 6664501 NaN 0 NaN 2.26<br />
1426 RB12386_conserved hypothetical protein, secreted 6665667 6665852 0 0 0 1.96<br />
1427 RB12387_conserved hypothetical protein 6665894 6665742 NaN 0 NaN 2.35<br />
1432 RB12392_conserved hypothetical protein 6668543 6668436 NaN 0 0 2.31<br />
1433 RB12393_c-type cytochrome precursor 6670561 6668555 NaN 0 NaN 2.23<br />
1439 RB12403_conserved hypothetical protein 6673741 6673562 0 0 0 2.08<br />
1440 RB12404_conserved hypothetical protein 6674021 6673728 0 0 0 2.15<br />
1447 RB12416_secreted protein 6679578 6680456 0 0 0 2.66<br />
1462 RB12439_protein containing DUF1586 6695571 6695437 0 0 0 1.84<br />
1487 RB12479_conserved hypothetical protein 6717557 6716985 0 0 0 2.3<br />
1491 RB12484_conserved hypothetical protein, membrane 6721052 6720078 0 0 0 1.92<br />
1525 RB1254_hypothetical protein 640610 640747 NaN 0 0 1.63<br />
1527 RB12543_conserved hypothetical protein, membrane 6759514 6758519 NaN 0 0 1.62<br />
1591 RB12648_conserved hypothetical protein 6810287 6810496 NaN 0 0 1.93<br />
1613 RB12684_conserved hypothetical protein 6831038 6830874 0 0 0 1.54<br />
1634 RB12716_conserved hypothetical protein 6849459 6849115 0 0 0 1.68<br />
1639 RB12727_conserved hypothetical protein 6860301 6858259 NaN 0 NaN 2.18<br />
1643 RB12737_conserved hypothetical protein 6863917 6864114 0 0 0 2.39<br />
1644 RB12739_conserved hypothetical protein 6864276 6864106 0 0 0 2.91<br />
1674 RB12782_conserved hypothetical protein 6889986 6889819 NaN 0 0 3.95<br />
1692 RB12810_conserved hypothetical protein 6912339 6912542 NaN 0 NaN 2.1<br />
1697 RB12815_conserved hypothetical protein 6914656 6914525 0 1.46 0 2.4<br />
1698 RB12816_glutamine amidotransferase or related peptidase 6914710 6915483 NaN 0 0 1.6<br />
1699 RB12818_conserved hypothetical protein 6915989 6915498 1.59 0 0 4.91<br />
1705 RB12827_conserved hypothetical protein, membrane 6919729 6919361 0 -1.69 0 2.07<br />
1706 RB12829_conserved hypothetical protein 6919813 6920292 NaN 0 0 2.28<br />
1710 RB12836_conserved hypothetical protein 6921619 6921735 0 0 0 2.07<br />
1730 RB12863_conserved hypothetical protein 6934850 6935002 NaN 0 0 2.07<br />
1748 RB12895_conserved hypothetical protein 6945636 6946097 NaN 0 NaN 2.39<br />
1776 RB12959_conserved hypothetical protein 6974205 6973882 0 0 0 1.77<br />
1814 RB13025_monooxygenase 6997019 6996438 0 0 0 2.58<br />
1883 RB13139_xylose operon regulatory protein 7049875 7048625 0 0 NaN 2.21<br />
1917 RB13198_conserved hypothetical protein 7084995 7085369 0 0 0 1.64<br />
1934 RB13224_nucleoside-diphosphate-sugar epimerase 7096270 7094813 0 0 0 1.64<br />
1936 RB13228_conserved hypothetical protein 7097952 7098302 NaN 0 0 1.82<br />
1947 RB13244_conserved hypothetical protein 7103330 7103061 0 0 0 2.11<br />
1949 RB13247_conserved hypothetical protein 7104177 7103620 0 0 0 1.47<br />
1996 RB1336_hypothetical protein 679357 680019 NaN 0 NaN 2.12<br />
2053 RB1433_Na(+):H(+) antiporter subunit B (Multiple resistance and pH homeostasis protein B) 732848 732411 0 0 0 1.98<br />
2072 RB1463_hypothetical protein 743584 743886 0 0 0 1.65<br />
2090 RB1491_hypothetical protein 766600 766265 0 0 0 2.79
2099 RB1507_tRNA isopentenyltransferase 772202 771198 2.5.1.8 0 0 NaN 2.44<br />
2102 RB1511_hypothetical protein 774995 773376 NaN 0 NaN 2.14<br />
2133 RB1565_hypothetical protein 799552 799322 NaN 0 NaN 2.45<br />
2155 RB1601_hypothetical protein 824863 824636 0 0 0 2.38<br />
2157 RB1603_hypothetical protein 825177 824944 0 0 NaN 2.23<br />
2168 RB1621_Phosphoesterase, PA-phosphatase related 835361 834309 NaN 0 NaN 2.28<br />
2250 RB1778_hypothetical protein 921245 921036 0 0 0 3.15<br />
2276 RB1830_hypothetical protein 945611 945498 NaN 0 NaN 3.07<br />
2294 RB1860_hypothetical protein 960361 960693 0 0 0 1.7<br />
2315 RB1896_membrane protein containing DUF1294 985295 985615 NaN 0 0 2.94<br />
2326 RB1910_hypothetical protein 993764 993393 0 -2.39 0 4.07<br />
2397 RB2043_hypothetical protein 1076233 1076418 0 0 0 2.36<br />
2425 RB2090_hypothetical protein 1101973 1102308 NaN 0 0 1.45<br />
2452 RB2140_hypothetical protein 1131458 1131607 0 0 0 1.71<br />
2456 RB2144_g eranylgeranyl pyrophosphate synthetase 1136089 1135136 0 0 0 1.57<br />
2472 RB2173_hypothetical protein 1147959 1146895 0 0 NaN 2.99<br />
2481 RB2185_hypothetical protein 1152681 1152529 NaN 0 NaN 2.6<br />
2482 RB2186_ISXo8 transposase 1153993 1152692 NaN 0 NaN 2.37<br />
2519 RB2252_hypothetical protein 1181701 1181540 NaN 0 0 1.72<br />
2534 RB227_hypothetical protein 120858 121037 0 0 0 1.54<br />
2559 RB2310_hypothetical protein 1209545 1209222 0 0 0 2.05<br />
2572 RB2330_secreted protein 1214565 1214014 0 0 0 2.65<br />
2621 RB2398_conserved hypothetical protein 1248404 1247520 0 0 0 1.99<br />
2625 RB2401_Phosphoesterase, PA-phosphatase related 1248679 1250799 0 0 0 2.51<br />
2654 RB2457_hypothetical protein 1279788 1280144 0 0 0 2.24<br />
2672 RB2477_hypothetical protein 1295793 1295422 2.51 0 0 1.79<br />
2673 RB2478_hypothetical protein 1295756 1295872 0 0 0 2.72<br />
2691 RB2498_methyltransferase, 1305777 1304887 NaN 0 0 1.84<br />
2700 RB2507_dTDP-glucose 4,6-dehydratase 1313064 1311886 4.2.1.46 0 0 NaN 2.54<br />
2702 RB2509_hypothetical protein 1313237 1313377 0 0 0 1.68<br />
2705 RB2511_hypothetical protein 1313530 1313667 NaN 0 NaN 2.15<br />
2707 RB2513_hypothetical protein 1314624 1314764 NaN 0 0 1.57<br />
2717 RB2524_hypothetical protein 1319449 1319796 NaN 0 0 2.27<br />
2723 RB2534_hypothetical protein 1324897 1325199 0 0 0 2.03<br />
2727 RB2545_hypothetical protein 1328931 1328773 0 0 0 3.27<br />
2734 RB2556_Planctomycete PGAMP 1335620 1335438 NaN 0 NaN 2.3<br />
2750 RB2585_Bacterial microcompartments protein 1346035 1345766 NaN 0 0 2.26<br />
2751 RB2586_Bacterial microcompartments protein 1346402 1346106 0 0 0 1.97<br />
2774 RB2627_Glyceraldehyde 3-phosphate dehydrogenase 1364551 1365579 1.2.1.12 0 0 0 1.61<br />
2783 RB2640_Sugar phosphate isomerase/epimerase 1372769 1371720 0 0 0 1.95<br />
2784 RB2642_GTP cyclohydrolase I 1373147 1372782 3.5.4.16 0 0 0 1.49<br />
2844 RB2740_hypothetical protein 1430028 1429648 NaN 0 0 1.6<br />
2850 RB2747_hypothetical protein 1432443 1432685 0 0 0 2.23<br />
2851 RB2748_conserved hypothetical protein 1433804 1432641 0 0 0 1.78<br />
2852 RB2749_4-hydroxybenzoate octaprenyltransferase 1434783 1433866 2.5.1.- 0 0 0 1.67
2860 RB2764_secreted protein containing DUF1585 1442155 1439630 0 0 -1.68 2.66<br />
2867 RB2784_membrane protein containing DUF107 1448065 1447454 NaN 0 0 2.16<br />
2871 RB279_ABC-type multidrug transport system, ATPase component 138584 139510 NaN 0 NaN 2.28<br />
2923 RB2875_hypothetical protein 1497313 1497534 0 0 0 1.58<br />
2951 RB2924_secreted protein 1515456 1515025 0 0 0 1.53<br />
2965 RB2943_hypothetical protein 1521275 1521535 0 0 0 2.02<br />
2991 RB2976_ATP:cob(I)alamin adenosyltransferase 1536008 1535418 0 0 0 1.65<br />
2998 RB2985_glycosyl transferase, group 1 family protein 1542040 1540943 0 0 NaN 2.36<br />
3018 RB3013_hypothetical protein 1563386 1563619 NaN 0 NaN 2.14<br />
3057 RB3073_hypothetical protein 1585180 1585025 0 0 0 2.37<br />
3082 RB314_malonyl CoA-acyl carrier protein transacylase 156968 157897 2.3.1.39 0 0 0 2.19<br />
3083 RB3140_hypothetical protein 1620838 1621173 0 0 0 1.68<br />
3088 RB3146_protein containing DUF1556 1623952 1624248 0 0 NaN 2.74<br />
3094 RB3153_hypothetical protein (protein containing DUF1584) 1626911 1626681 1.83 0 0 2.3<br />
3100 RB316_3-oxoacyl-[acyl-carrier-protein] reductase 158031 158789 1.1.1.100 0 0 0 2.36<br />
3104 RB3172_hypothetical protein 1634919 1634326 0 0 0 1.46<br />
3111 RB3189_hypothetical protein 1646085 1646393 0 -1.47 0 2.06<br />
3135 RB3224_hypothetical protein 1656004 1656450 -1.58 0 0 1.97<br />
3144 RB324_hypothetical protein 162731 162880 0 0 0 2.07<br />
3149 RB3247_hypothetical protein 1668665 1668390 0 0 0 1.86<br />
3160 RB3261_hypothetical protein 1678001 1677717 0 0 0 1.78<br />
3207 RB3333_hypothetical protein 1713409 1713528 0 0 0 2.03<br />
3217 RB335_hypothetical protein 168642 168385 0 0 0 2.06<br />
3260 RB341_hypothetical protein 169852 170124 1.67 0 NaN 2.27<br />
3286 RB3449_hypothetical protein 1782475 1782317 0 0 0 1.6<br />
3379 RB3599_KLHL5 protein 1871888 1872985 -1.47 0 0 2.28<br />
3406 RB3633_hypothetical protein 1890483 1890211 0 0 0 1.67<br />
3425 RB3659_hypothetical protein 1900428 1900607 0 0 0 2.63<br />
3428 RB3662_hypothetical protein 1902024 1901893 NaN 0 0 2.03<br />
3429 RB3663_secreted protein 1902328 1902119 0 0 0 1.87<br />
3567 RB3864_hypothetical protein 1998657 1999049 0 0 0 2.16<br />
3568 RB3865_acetyltransferase, GNAT family 1999065 1999601 0 0 0 1.66<br />
3750 RB4160_conserved hypothetical protein 2136438 2137598 0 0 0 2.23<br />
3755 RB4167_secreted cytochrome c-like protein 2144846 2141907 0 0 0 1.93<br />
3769 RB4194_hypothetical protein 2156685 2156524 0 0 0 2.43<br />
3851 RB4306_transposase and inactivated derivative 2221207 2219834 NaN 0 0 1.63<br />
3862 RB4325_Planctomycete PGAMP 2228477 2228623 NaN 0 NaN 3.77<br />
3867 RB4338_hypothetical protein 2233809 2233636 0 -1.57 0 2.78<br />
3926 RB4425_hypothetical protein 2275100 2275483 0 0 0 1.81<br />
3937 RB4440_Amidohydrolase 2 2278882 2279925 4.1.1.45 NaN 0 0 1.63<br />
3947 RB4457_hypothetical protein 2285628 2285924 0 0 0 2.39<br />
3967 RB4482_hypothetical protein 2297240 2296905 0 -1.67 0 2.53<br />
3976 RB4498_hypothetical protein 2303522 2303250 1.51 0 0 2.2<br />
4001 RB4530_heavy metal efflux pump, CzcA family 2323867 2320475 0 0 0 3.17<br />
4024 RB4572_hypothetical protein 2339375 2339103 0 0 0 2.35
4044 RB4601_protein containing DUF1559 2356778 2357734 0 0 0 2.1<br />
4081 RB4652_hypothetical protein 2380494 2380652 0 0 -1.59 1.9<br />
4090 RB4662_secreted protein 2383563 2383676 -1.49 0 0 3.35<br />
4104 RB4691_hypothetical protein 2395001 2395855 0 0 0 2.51<br />
4114 RB4705_hypothetical protein 2403864 2403694 0 0 0 1.62<br />
4164 RB4772_hypothetical protein 2446610 2446741 0 0 0 1.48<br />
4169 RB4781_HesB/YadR/YfhF 2449655 2450125 0 0 NaN 2.2<br />
4205 RB483_hypothetical protein 262569 262733 0 0 0 2.06<br />
4287 RB4957_hypothetical protein 2548380 2548553 0 0 0 2.15<br />
4296 RB4969_hypothetical protein 2554443 2555447 0 0 0 2.02<br />
4340 RB5029_conserved hypothetical protein, secreted 2589744 2589034 0 0 0 2.46<br />
4378 RB5093_hypothetical protein 2619010 2619213 NaN 0 0 1.89<br />
4407 RB5138_hypothetical protein 2641024 2640848 0 0 0 2.71<br />
4424 RB5168_hypothetical protein 2658508 2658690 0 0 0 1.79<br />
4443 RB5195_arylsulfatase 2674328 2675845 3.1.6.12 0 0 0 1.53<br />
4507 RB5294_sulfatase 2732584 2730983 0 0 0 1.54<br />
4536 RB5348_transposase and inactivated derivative 2765787 2764414 0 0 0 1.46<br />
4552 RB5371_hypothetical protein 2774829 2775158 0 0 0 1.77<br />
4614 RB546_secreted protein containing DUF1501 302834 304291 0 0 0 3.01<br />
4641 RB5502_membrane protein 2845859 2846293 0 0 0 2.24<br />
4683 RB5572_hypothetical protein 2901618 2901499 0 0 0 1.94<br />
4695 RB5597_conserved hypothetical protein, secreted 2913295 2913807 NaN 0 0 2.93<br />
4697 RB560_protein containing DGPFAETKE domain 309384 308542 0 0 0 2.24<br />
4847 RB5846_glycosyltransferase 3064164 3065057 0 0 0 1.55<br />
4864 RB5874_hypothetical protein 3080941 3080798 0 0 0 1.69<br />
4867 RB5877_hypothetical protein 3082302 3082409 0 0 0 1.67<br />
4901 RB5937_hypothetical protein 3112147 3113067 0 0 0 2.85<br />
4917 RB5966_hypothetical protein 3128115 3128537 0 0 0 1.58<br />
4931 RB5988_secreted protein 3141494 3140970 0 0 0 2.21<br />
4933 RB5990_hypothetical protein 3141918 3141577 NaN 0 NaN 2.31<br />
4985 RB6081_hypothetical protein 3191035 3190877 0 0 0 1.52<br />
5022 RB6147_3-dehydroquinate synthase 3229258 3230442 4.2.3.4 0 0 0 1.52<br />
5035 RB6168_hypothetical protein 3236689 3237315 NaN 0 NaN 2.3<br />
5038 RB6171_site-specific recombinase 3239091 3238048 0 0 0 1.45<br />
5041 RB6175_hypothetical protein 3240125 3239748 0 0 NaN 2.18<br />
5046 RB6188_hypothetical protein 3244384 3244178 NaN 0 NaN 3.01<br />
5080 RB6245_hypothetical protein 3278762 3279085 0 0 0 1.93<br />
5088 RB6257_secreted protein 3288666 3288043 0 0 0 3.14<br />
5157 RB6374_conserved hypothetical protein 3348288 3349616 NaN 0 NaN 2.97<br />
5158 RB6375_hypothetical protein 3349681 3350574 1.86 0 0 2.72<br />
5165 RB6382_hypothetical protein 3355162 3355043 0 0 0 2.35<br />
5172 RB6390_hypothetical protein 3361959 3362219 0 0 0 1.94<br />
5180 RB6405_hypothetical protein 3371368 3371087 0 0 0 1.91<br />
5203 RB6440_hypothetical protein 3391656 3391829 0 0 0 1.62<br />
5316 RB6619_hypothetical protein 3518291 3517503 0 0 0 1.66
5339 RB6661_hypothetical protein 3538752 3538486 0 0 0 1.54<br />
5365 RB670_hypothetical protein 359315 358905 0 0 0 1.65<br />
5373 RB671_hypothetical protein 359218 359586 0 0 0 2.48<br />
5379 RB6720_hypothetical protein 3567752 3567970 0 0 0 2.08<br />
5390 RB6736_ISXo8 transposase 3578852 3577551 NaN 0 NaN 2.85<br />
5405 RB6761_bacterioferritin comigratory protein 3594163 3594759 0 0 0 1.72<br />
5445 RB6822_Chorismate synthase 3623410 3624624 4.2.3.5 NaN 0 NaN 2.88<br />
5480 RB6883_Lipoprotein releasing system ATP-binding protein lolD 3657855 3658622 NaN 0 NaN 2.31<br />
5500 RB6916_hypothetical protein 3676188 3676391 0 0 NaN 2.5<br />
5508 RB6930_glutamate dehydrogenase 3680317 3679076 1.4.1.3 2.24 -1.94 0 3.1<br />
5509 RB6932_CYSTEINE SYNTHASE A 3681308 3680349 2.5.1.47 NaN 0 0 2.97<br />
5538 RB6989_hypothetical protein 3719206 3718958 NaN 0 NaN 2.29<br />
5557 RB7016_hypothetical protein 3732513 3733238 NaN 0 NaN 2.59<br />
5620 RB7114_phenylalanyl-tRNA synthetase alpha chain 3779252 3778209 6.1.1.20 0 0 0 1.67<br />
5635 RB7136_hypothetical protein 3787932 3788138 NaN 0 0 1.64<br />
5657 RB7176_hypothetical protein 3806783 3806643 0 0 0 1.56<br />
5735 RB7292_metallo-beta-lactamase 3875577 3876347 0 -1.48 0 2.35<br />
5753 RB7318_hypothetical protein 3895264 3894884 0 0 0 3<br />
5754 RB7319_hypothetical protein 3895491 3895261 0 0 0 2.55<br />
5778 RB7389_Putative transposase 3953590 3954717 NaN 0 NaN 2.21<br />
5779 RB7390_hypothetical protein 3954342 3954968 0 0 0 1.57<br />
5869 RB754_Polysaccharide export protein 403211 404305 NaN 0 NaN 2.09<br />
5915 RB7599_hypothetical protein 4086498 4086379 0 0 0 1.84<br />
5952 RB7652_malate dehydrogenase 4112505 4113452 1.1.1.37 0 0 NaN 2.84<br />
5966 RB7678_protocatechuate 3,4-dioxygenase type II beta subunit 4123339 4122659 0 0 0 1.89<br />
6042 RB7790_hypothetical protein 4173233 4173502 0 -1.49 0 1.88<br />
6095 RB7875_Arylsulfatase A precursor 4208176 4209654 3.1.6.8 0 0 0 2.32<br />
6213 RB8084_hypothetical protein 4330006 4329890 0 0 0 2.16<br />
6227 RB8107_secreted protein 4339329 4340276 0 0 0 1.84<br />
6237 RB8127_hypothetical protein 4349191 4349979 NaN 0 0 1.69<br />
6242 RB8138_hypothetical protein 4351960 4352187 0 0 0 2<br />
6312 RB8260_protein containing DUF167 4415394 4415068 0 0 0 1.56<br />
6320 RB8275_hypothetical protein 4424897 4424760 NaN 0 0 1.61<br />
6322 RB8279_hypothetical protein 4425128 4425343 0 0 -1.49 2.19<br />
6369 RB8357_hypothetical protein 4460174 4460004 1.72 0 0 1.95<br />
6431 RB8468_hypothetical protein 4520799 4520533 0 0 0 1.78<br />
6472 RB8530_hypothetical protein 4550886 4550725 0 0 0 1.75<br />
6547 RB867_hypothetical protein 460731 460429 0 0 0 2.02<br />
6548 RB8670_hypothetical protein 4614842 4614985 2.47 0 0 4.21<br />
6564 RB8697_hypothetical protein 4630281 4630111 NaN 0 0 3.26<br />
6567 RB8700_hypothetical protein 4630224 4630400 1.51 -2.34 0 3.77<br />
6588 RB8739_hypothetical protein 4643832 4644191 0 0 0 1.49<br />
6595 RB8747_DNA/pantothenate metabolism flavoprotein 4648152 4648808 NaN 0 NaN 2.08<br />
6596 RB8748_dihydroorotate dehydrogenase 4648815 4649732 1.3.3.1 NaN 0 NaN 2.33<br />
6636 RB8818_hypothetical protein 4689021 4688839 0 0 0 2.13
6650 RB8840_acylphosphatases 4701445 4701729 NaN 0 NaN 2.09<br />
6672 RB8876_hypothetical protein 4723629 4723892 0 0 0 1.63<br />
6678 RB8884_hypothetical protein 4727666 4727878 0 0 NaN 2.26<br />
6691 RB8902_hypothetical protein 4740474 4740226 0 0 0 2.3<br />
6700 RB8917_hypothetical protein 4747435 4747659 0 0 0 2.59<br />
6731 RB8975_hypothetical protein 4783888 4783556 NaN 0 0 2.6<br />
6737 RB8983_methanol dehydrogenase regulatory protein 4788960 4790000 0 0 0 1.68<br />
6763 RB9037_secreted protein 4821537 4822571 NaN 0 0 1.68<br />
6764 RB9038_hypothetical protein 4823103 4822642 0 0 0 2.74<br />
6782 RB9078_2-oxoglutarate dehydrogenase E1 component 4846881 4849790 1.2.4.2 NaN 0 0 1.55<br />
6849 RB9196_secreted protein containing DUF1080 4916635 4917975 0 0 NaN 2.11<br />
6851 RB9198_hypothetical protein 4918032 4918208 NaN 0 NaN 5.13<br />
6871 RB9231_hypothetical protein 4937008 4936679 NaN 0 NaN 2.16<br />
6879 RB924_hypothetical protein 490400 489774 NaN 0 NaN 2.86<br />
6897 RB9276_membrane protein 4958461 4959162 0 0 0 1.57<br />
6929 RB933_hypothetical protein 493451 492825 NaN 0 NaN 2.25<br />
6933 RB934_Putative transposase 494203 493076 NaN 0 NaN 2.17<br />
6950 RB936_hypothetical protein 494524 494267 0 0 0 1.59<br />
6953 RB9365_hypothetical protein 5017351 5017809 0 0 0 3<br />
7157 RB9699_Rhs element Vgr protein 5226252 5228345 0 0 0 1.48<br />
7159 RB9702_conserved hypothetical protein 5229342 5229632 0 0 NaN 2.66<br />
7277 RB9908_hypothetical protein 5339497 5339649 NaN 0 NaN 2.15<br />
7283 RB9918_hypothetical protein 5344369 5344872 NaN 0 NaN 2.75<br />
7292 RB9936_hypothetical protein 5350464 5350640 0 0 0 1.48
Cluster 4<br />
Original row UID Start Stop EC 62 h vs 44 h 82 h va 62 h 96 h vs 82 h 240 h vs 82 h<br />
567 RB10951_secreted protein 5940232 5939927 3.24 0 1.51 -3.11<br />
207 RB10341_hypothetical protein 5593416 5593601 2.27 0 0 0<br />
209 RB10343_hypothetical protein 5593898 5594059 3.62 0 0 0<br />
264 RB10440_hypothetical protein 5655693 5655842 3.33 0 0 -2<br />
393 RB10665_hypothetical protein 5789912 5790514 1.47 1.47 0 0<br />
619 RB1104_hypothetical protein 572078 572257 2.89 0 0 0<br />
1303 RB12199_conserved hypothetical protein 6577614 6577778 1.58 0 0 0<br />
1567 RB12610_conserved hypothetical protein 6793700 6793957 2.12 0 0 -2.19<br />
1709 RB12833_conserved hypothetical protein 6921305 6921634 1.85 0 0 0<br />
1869 RB13115_conserved hypothetical protein 7036614 7036369 2.31 0 0 -2.5<br />
2349 RB1961_hypothetical protein 1033791 1034144 3.07 0 0 NaN<br />
3118 RB3196_hypothetical protein 1650093 1650422 1.65 0 0 0<br />
3147 RB3244_hypothetical protein 1667233 1666949 1.47 0 0 -1.47<br />
3237 RB3374_hypothetical protein 1740350 1740063 1.5 0 -1.75 -2.37<br />
3411 RB3644_hypothetical protein 1895184 1894801 2.98 -1.6 -2.27 0<br />
3977 RB4499_hypothetical protein 2303764 2303519 2.06 0 0 0<br />
4897 RB5929_hypothetical protein 3107256 3107480 1.86 0 0 0<br />
5160 RB6378_hypothetical protein 3352598 3353284 2.75 0 0 -1.99<br />
5214 RB6454_hypothetical protein 3407776 3407495 1.67 0 0 0<br />
5496 RB6910_hypothetical protein 3671484 3671356 3.07 0 0 -3.12<br />
6065 RB7828_hypothetical protein 4192037 4191915 2.96 0 0 0<br />
6247 RB8146_hypothetical protein 4355122 4354667 2.1 0 0 -2.21<br />
6268 RB8188_hydroxypyruvate isomerase 4378573 4379352 5.3.1.22 2.3 0 NaN 0<br />
6546 RB8669_hypothetical protein 4614557 4614670 2.61 -1.57 0 1.69<br />
6609 RB8775_hypothetical protein 4665497 4665252 2.49 0 0 0<br />
7006 RB9450_hypothetical protein 5076581 5076739 3.13 -1.46 NaN 1.89
Cluster 11<br />
Original row UID Start Stop EC 62 h vs 44 h 82 h va 62 h 96 h vs 82 h 240 h vs 82 h<br />
206 RB10339_RNA polymerase sigma factor RpoD 5593398 5591839 NaN 0 0 -1.89<br />
3919 RB4416_Cytochrome c oxidase, subunit I 2271803 2269302 -1.85 0 0 -1.71<br />
6797 RB9104_hypothetical protein 4866696 4866559 -1.46 0 0 -1.73<br />
142 RB10221_conserved hypothetical protein 5521594 5522802 NaN 0 0 -1.5<br />
153 RB10242_Curved DNA-binding protein 5536118 5535126 NaN 2.44 0 -2.37<br />
229 RB10384_hypothetical protein 5612174 5611884 NaN 2.44 0 -3.18<br />
256 RB1043_conserved hypothetical protein 543083 543352 NaN 0 0 -2.15<br />
326 RB10553_succinate dehydrogenase (cytochrome b558 subunit) 5723185 5724069 NaN 0 0 -1.75<br />
566 RB10950_membrane or secreted protein 5939124 5939825 NaN 0 0 -2.29<br />
598 RB11000_conserved hypothetical protein, membrane 5964054 5963380 0 1.49 0 -2.27<br />
702 RB11183_universal stress protein family 6068240 6067293 NaN 0 0 -1.46<br />
736 RB11235_hypothetical protein 6090398 6090991 NaN 0 0 -1.66<br />
860 RB11432_conserved hypothetical protein 6197188 6196988 NaN 0 0 -1.47<br />
1099 RB11853_GTP cyclohydrolase I 6382501 6383190 3.5.4.16 NaN 0 0 -1.49<br />
1106 RB11863_RecA bacterial DNA recombination protein 6388538 6387411 NaN 1.63 0 -2.03<br />
1118 RB11886_protein containing DUF1585 6403682 6400881 0 0 0 -1.85<br />
1225 RB12060_conserved hypothetical protein 6507405 6506977 NaN 1.47 0 -1.59<br />
1367 RB12296_Short-chain dehydrogenase/reductase SDR 6615422 6614403 NaN 1.66 0 -1.58<br />
1417 RB12372_RNA polymerase ECF-type sigma factor 6658702 6659430 NaN 0 0 -2.48<br />
1435 RB12396_ hypothetical protein 6670870 6670697 0 0 0 -1.66<br />
1483 RB12471_conserved hypothetical protein 6712442 6712185 NaN 1.82 0 -1.84<br />
1551 RB12581_conserved hypothetical protein 6780597 6780073 -1.65 0 0 -1.48<br />
1573 RB12621_conserved hypothetical protein 6796861 6796604 NaN 0 0 -1.82<br />
1596 RB12658_3-isopropylmalate dehydratase small subunit 6814851 6815441 4.2.1.33 NaN 0 0 -1.54<br />
1756 RB1291_hypothetical protein 656684 656977 -1.74 0 NaN -1.68<br />
1990 RB13324_hypothetical protein 7141677 7141787 -1.74 0 NaN -1.52<br />
2013 RB1359_Protease do precursor 693488 695377 3.4.21.- NaN 0 0 -1.86<br />
2023 RB138_Sigma factor, ECF-like 60872 61516 NaN 0 0 -1.73<br />
2030 RB1392_RNA polymerase sigma factor 711705 710683 NaN 0 0 -1.85<br />
2131 RB1561_Ribulose-phosphate 3-epimerase 798959 798252 5.1.3.1 NaN 0 0 -1.53<br />
2536 RB2274_hypothetical protein 1193331 1192939 0 0 -1.47 -1.7<br />
2557 RB2306_hypothetical protein 1206782 1206907 -1.68 0 NaN -1.47<br />
2573 RB2331_hypothetical protein 1215088 1214687 0 0 0 -1.51<br />
2674 RB2479_conserved hypothetical protein 1296683 1295862 -1.59 0 NaN -1.52<br />
2786 RB2647_conserved hypothetical protein, secreted 1374889 1374116 NaN 0 0 -2.04<br />
2787 RB2649_hypothetical protein 1374987 1375148 0 1.55 0 -2.48
2883 RB2810_hypothetical protein 1459901 1459752 NaN 0 0 -5.37<br />
2982 RB2963_hypothetical protein 1526707 1527066 NaN 1.62 0 -1.98<br />
2990 RB2975_Nitrogen regulatory protein P-II 1535384 1534983 NaN 0 0 -1.62<br />
3022 RB3023_secreted protein 1566881 1566414 0 0 0 -2.32<br />
3174 RB3281_Histone-like bacterial DNA-binding protein 1687272 1686853 -1.53 0 NaN -1.62<br />
3248 RB3395_secreted protein containing DUF1559 1752151 1753347 0 0 0 -1.47<br />
3301 RB3473_membrane protein 1794413 1795744 NaN 0 0 -1.47<br />
3402 RB3627_Transglutaminase-like domain 1888482 1887640 -1.65 0 NaN -1.7<br />
3518 RB3789_hypothetical protein 1962878 1962399 NaN 0 0 -3.06<br />
3532 RB3813_hypothetical protein 1975188 1975415 0 0 0 -1.59<br />
3580 RB3882_hypothetical protein 2007369 2007217 0 0 0 -1.64<br />
3595 RB3905_hypothetical protein 2018601 2018717 -1.89 0 0 -1.77<br />
3610 RB3928_hypothetical protein 2033789 2034310 0 0 0 -1.55<br />
3629 RB3953_hypothetical protein 2048784 2046211 -2 0 NaN -1.72<br />
3681 RB4032_secreted protein containing DUF1501 2084885 2083440 -1.58 0 0 -1.46<br />
3698 RB4071_integral membrane protein 2098441 2099550 0 0 0 -1.93<br />
3711 RB4097_conserved hypothetical protein 2108244 2110445 -1.88 0 0 -1.76<br />
3753 RB4165_hypothetical protein 2139632 2141797 -1.57 0 NaN -1.64<br />
3756 RB417_nonspecific nucleoside hydrolase 221324 222268 NaN 0 0 -2.05<br />
3760 RB4176_hypothetical protein 2146928 2146638 0 1.9 0 -3.42<br />
3763 RB418_conserved hypothetical protein, membrane 222222 223313 -1.96 0 NaN -1.96<br />
3771 RB4196_hypothetical protein 2157140 2157484 -1.47 0 NaN -1.52<br />
3795 RB4229_conserved hypothetical protein 2167854 2166595 -1.48 0 0 -1.46<br />
3810 RB4255_hypothetical protein 2180806 2180192 0 0 0 -1.65<br />
3904 RB4394_protease I 2261372 2261938 3.2.-.- -1.7 0 1.61 -3.87<br />
3906 RB4396_glutathione reductase 2262532 2263887 1.8.1.7 NaN 0 0 -1.56<br />
3944 RB4454_Flagellin A 2283201 2285420 -1.54 0 0 -1.78<br />
3949 RB4459_MscS Mechanosensitive ion channel 2288477 2287089 -1.52 0 0 -1.72<br />
3982 RB4504_membrane protein containing DUF421 2304293 2304838 NaN 0 0 -1.52<br />
4004 RB4538_secreted protein 2326219 2325701 NaN 0 0 -1.6<br />
4054 RB4613_HesB/YadR/YfhF 2362510 2362178 -1.59 2.48 0 -3.39<br />
4055 RB4614_hypothetical protein 2362625 2362491 -1.48 0 0 -3.76<br />
4097 RB4678_secreted protein 2390618 2391217 NaN 1.53 0 -1.85<br />
4153 RB4754_hypothetical protein 2437589 2437765 NaN 0 0 -2.12<br />
4234 RB4876_delta 9 acyl-lipid fatty acid desaturase 2506214 2505021 1.14.19.- NaN 0 0 -3.63<br />
4250 RB4900_hypothetical protein 2520067 2519567 NaN 0 0 -1.59<br />
4288 RB4958_hypothetical protein 2549283 2548531 NaN 0 0 -1.77<br />
4387 RB5103_membrane protein 2622260 2623318 0 0 0 -1.67
4487 RB5262_membrane protein 2713742 2714029 NaN 0 0 -1.99<br />
4596 RB5434_Elongation factor G 1 2817863 2815776 -1.51 0 0 -1.47<br />
4738 RB566_exopolysaccharide synthesis, ExoD family 312007 311123 0 0 0 -2.41<br />
4798 RB5761_conserved hypothetical protein, membrane 3015202 3014426 -1.55 0 NaN -1.68<br />
4824 RB5801_Ribosomal protein S15 3039583 3039852 NaN 0 0 -1.51<br />
4872 RB5882_hypothetical protein 3084330 3083599 NaN 0 0 -2.14<br />
4911 RB5955_hypothetical protein 3121488 3122021 NaN 0 0 -2.57<br />
4948 RB6010_hypothetical protein 3149911 3150072 -1.65 0 NaN -1.64<br />
5118 RB6314_hypothetical protein 3319320 3319478 0 0 -1.88 -2.65<br />
5143 RB6351_hypothetical protein 3336171 3336347 NaN 0 0 -1.74<br />
5189 RB6417_conserved hypothetical protein 3378045 3378794 NaN 2.23 0 -2.97<br />
5219 RB6463_conserved hypothetical protein, membrane 3415155 3415724 0 0 0 -1.65<br />
5294 RB658_transport protein 356228 354990 NaN 0 0 -1.7<br />
5296 RB6583_secreted protein 3498012 3501002 0 0 -1.62 -1.92<br />
5338 RB666_conserved hypothetical protein 357735 357956 NaN 0 0 -1.46<br />
5352 RB6681_hypothetical protein 3551775 3552194 0 0 0 -1.55<br />
5381 RB6724_hypothetical protein 3569189 3569584 -1.54 0 NaN -1.86<br />
5408 RB6766_hypothetical protein 3597367 3597534 0 0 0 -1.5<br />
5415 RB6778_hypothetical protein 3603142 3603321 0 0 0 -2.49<br />
5485 RB6895_Glycosyl transferase, family 4 3666358 3665153 NaN 0 0 -1.62<br />
5504 RB6923_hypothetical protein 3677883 3678398 NaN 0 0 -1.7<br />
5514 RB6941_secreted protein 3686675 3686076 NaN 0 0 -1.67<br />
5598 RB7085_hypothetical protein 3763662 3763435 NaN 2.58 0 -4.43<br />
5820 RB7452_conserved hypothetical protein 3994713 3995225 NaN 1.78 0 -2.49<br />
6232 RB8119_hypothetical protein 4346427 4346855 -1.6 0 NaN -1.47<br />
6304 RB8246_conserved hypothetical protein 4409241 4408441 NaN 0 0 -1.45<br />
6344 RB8319_hypothetical protein 4442421 4442308 NaN 0 0 -1.83<br />
6532 RB8633_Aminotransferase class-III 4601126 4602520 2.6.1.- 0 0 0 -2.66<br />
6795 RB9101_secreted YceI like family protein 4863681 4863130 NaN 0 0 -1.47<br />
6819 RB9141_rRNA (guanine-N(2)-)-methyltransferase 4891836 4892876 -1.5 0 NaN -1.51<br />
6967 RB9389_hypothetical protein 5035390 5034998 0 0 0 -2.2<br />
6969 RB9392_hypothetical protein 5038107 5037580 0 0 0 -1.86<br />
7002 RB9445_integral membrane protein 5074872 5075621 -1.78 0 NaN -1.85<br />
7040 RB951_protein containing DUF1596 502459 501755 0 0 0 -2.18<br />
7050 RB9529_hypothetical protein 5117956 5118297 -1.76 0 NaN -1.66<br />
7222 RB9811_secreted protein 5284365 5284673 0 0 0 -1.61<br />
7223 RB9813_hypothetical protein 5285123 5284638 NaN 0 0 -2.14<br />
176 RB10286_hypothetical protein 5558157 5558011 -1.5 0 0 -1.65
401 RB10678_hypothetical protein 5802466 5802816 -1.73 0 0 -1.57<br />
7127 RB9651_sialic acid-specific 9-O-acetylesterase 5198277 5196643 3.1.1.53 -2.07 0 NaN -1.6
Cluster 12<br />
Original row UID Start Stop EC 62 h vs 44 h 82 h va 62 h 96 h vs 82 h 240 h vs 82 h<br />
3229 RB3366_hypothetical protein 1730380 1730616 -1.46 0 NaN 0<br />
3704 RB4084_hypothetical protein 2104677 2105036 -1.49 0 NaN 0<br />
3780 RB4210_hypothetical protein 2160917 2161363 -1.45 0 NaN 0<br />
4267 RB4920_hypothetical protein 2531850 2531641 -2.34 0 0 0<br />
4756 RB5695_carbon-nitrogen hydrolase family 2971401 2972267 -1.69 0 NaN 0<br />
5155 RB6372_hypothetical protein 3346722 3347120 -1.48 0 NaN 0<br />
5468 RB686_arylsulfatase 370206 368557 3.1.6.1 -1.66 0 NaN 0<br />
102 RB1015_membrane protein 531723 532391 -1.62 0 NaN 0<br />
381 RB10648_conserved hypothetical protein 5779160 5778228 -1.61 0 NaN 0<br />
400 RB10675_membrane protein 5802507 5800168 -1.82 0 NaN 0<br />
596 RB110_choline-sulfatase 49093 47345 -1.5 0 NaN 0<br />
795 RB11325_conserved hypothetical protein 6142535 6142392 -1.92 0 NaN 0<br />
818 RB1137_hypothetical protein 585204 585488 -1.55 0 NaN 0<br />
1340 RB12250_conserved hypothetical protein 6597248 6597562 -1.53 0 0 0<br />
1397 RB12334_Peptidase M50 6636156 6635356 3.4.24.- -1.59 0 NaN 0<br />
1452 RB12426_conserved hypothetical protein, membrane 6684059 6684472 -1.53 0 NaN 0<br />
1518 RB12526_Rhomboid-like protein 6747132 6747902 -1.6 0 NaN 0<br />
1668 RB12770_conserved hypothetical protein 6884806 6884306 -2.26 0 0 0<br />
2336 RB1931_hypothetical protein 1013831 1013625 -1.96 0 NaN 0<br />
2436 RB2105_membrane protein 1114012 1112600 -1.54 0 0 0<br />
2438 RB2110_multidrug resistance protein norM 1114250 1115653 -1.52 0 NaN 0<br />
2603 RB2370_hypothetical protein 1229187 1229023 -1.59 0 NaN 0<br />
2667 RB2471_glycosyltransferase 1290824 1289514 -1.45 0 NaN 0<br />
2697 RB2503_O-antigen flippase 1311056 1309509 -1.61 0 0 0<br />
2719 RB2529_membrane protein 1320541 1321128 -1.74 0 0 0<br />
2757 RB2592_hypothetical protein 1348926 1348333 -1.57 0 NaN 0<br />
2976 RB2956_hypothetical protein 1524488 1524613 -1.48 0 NaN 0<br />
3089 RB3147_membrane protein 1624840 1624334 -1.72 0 NaN 0<br />
3171 RB3277_hypothetical protein 1686552 1685887 -1.5 0 0 0<br />
3204 RB3329_hypothetical protein 1711937 1711743 -1.66 0 NaN 0<br />
3235 RB3372_hypothetical protein 1733666 1734013 -1.72 0 0 0<br />
3262 RB3413_Esterase/lipase/ 1762402 1761281 -1.66 0 0 0<br />
3287 RB3450_hypothetical protein 1782543 1782358 -1.52 0 0 0<br />
3422 RB3656_polyktetide cyclase, secreted 1898389 1899330 -1.46 0 NaN 0<br />
3438 RB3675_secreted protein 1904552 1906780 -1.53 0 NaN 0<br />
3457 RB37_membrane protein 18328 17846 -1.61 0 NaN 0
3513 RB378_periplasmic nitrate reductase large subunit precursor 191963 194224 1.7.99.4 -1.46 0 0 0<br />
3650 RB3988_secreted protein 2059740 2060306 -1.45 0 NaN 0<br />
3693 RB406_arylsulfatase 217496 215823 -1.47 0 0 0<br />
3716 RB4104_hypothetical protein 2112335 2112553 -1.58 0 0 0<br />
3818 RB4266_hypothetical protein 2183250 2183414 -1.66 0 NaN 0<br />
3824 RB4273_iron-dependent peroxidase 2193431 2191875 1.1.1.- -1.77 0 NaN 0<br />
3868 RB434_hypothetical protein 231791 231988 -1.54 0 0 0<br />
3912 RB4402_hypothetical protein 2265340 2265501 -1.63 -1.51 NaN 0<br />
3948 RB4458_Protein of unknown function, UPF0118 2287089 2286061 -1.77 0 0 0<br />
3990 RB4513_serum resistance protein 2315098 2314118 -1.54 0 0 NaN<br />
4124 RB4719_hypothetical protein 2419276 2419112 -1.49 0 NaN 0<br />
4125 RB472_hypothetical protein 253266 253409 -1.76 0 -1.58 0<br />
4140 RB4738_dihydroorotate dehydrogenase 2430029 2429016 1.3.3.1 -1.47 0 NaN 0<br />
4165 RB4773_hypothetical protein 2446723 2447349 -1.87 0 -1.5 0<br />
4214 RB4844_hypothetical protein 2481716 2481868 -1.47 0 0 0<br />
4248 RB4897_hypothetical protein 2519505 2518951 -1.78 0 NaN 0<br />
4260 RB4913_ATP synthase C chain 2527319 2527648 3.6.3.14 -1.45 0 0 0<br />
4386 RB5102_hypothetical protein 2622248 2622000 -1.46 0 0 0<br />
4429 RB5174_Bacterial type II and III secretion system protein 2660936 2662705 -1.6 0 NaN 0<br />
4588 RB5424_sulfatase 2807339 2809060 -1.76 0 NaN 0<br />
4620 RB5468_O-antigen related protein 2831490 2830492 -1.48 0 NaN NaN<br />
4688 RB5581_conserved hypothetical protein 2905973 2904780 -1.68 0 NaN 0<br />
4760 RB5701_oxidase subunit 2975188 2978523 -1.5 0 NaN 0<br />
4846 RB5841_serine/threonine protein phosphatase family 3061924 3063936 -1.64 0 NaN 0<br />
4851 RB5851_hypothetical protein 3066676 3066461 -1.46 0 NaN 0<br />
4862 RB5872_hypothetical protein 3080059 3080310 -1.51 0 0 0<br />
4884 RB5903_hypothetical protein 3094315 3094491 -1.54 0 NaN 0<br />
4955 RB6019_hypothetical protein 3154683 3154198 -1.62 0 0 0<br />
4959 RB6031_hypothetical protein 3160462 3158984 -1.55 0 NaN 0<br />
4965 RB6042_CBS domain protein 3166072 3164825 -1.55 0 NaN 0<br />
5096 RB6274_hypothetical protein 3297130 3296951 -1.71 0 NaN 0<br />
5114 RB6305_dolichol-phosphate mannosyltransferase 3312842 3314155 -1.52 0 0 0<br />
5191 RB6423_hypothetical protein 3380603 3380740 -2.17 0 0 0<br />
5199 RB6434_hypothetical protein 3388682 3388536 -1.77 0 0 0<br />
5206 RB6443_Cys/Met metabolism pyridoxal-phosphate-dependent enzymes 3393276 3394469 -1.59 0 NaN 0<br />
5224 RB6470_Abortive infection protein 3420270 3422375 -1.63 -1.63 NaN 0<br />
5242 RB6496_hypothetical protein 3441948 3442121 -1.57 0 0 0<br />
5269 RB6537_Aldose 1-epimerase 3472890 3471736 5.1.3.3 -1.57 0 NaN 0
5271 RB6539_membrane protein 3474598 3475722 -1.75 0 NaN 0<br />
5287 RB6569_hypothetical protein 3489300 3489527 -1.47 0 NaN 0<br />
5290 RB6574_protein containing DUF1585 3493615 3491444 -2.19 0 NaN 0<br />
5298 RB6587_hypothetical protein 3501747 3501595 -1.92 0 0 0<br />
5313 RB6610_conserved hypothetical protein 3511694 3515401 -1.68 0 NaN 0<br />
5319 RB6624_Bacterial sugar transferase 3520589 3522100 2.7.8.6 -1.63 0 0 0<br />
5324 RB6632_hypothetical protein 3524963 3525130 -1.68 0 0 0<br />
5340 RB6663_Major facilitator superfamily MFS_1 3540255 3538870 -1.5 0 NaN 0<br />
5364 RB6699_hypothetical protein 3559781 3559924 -1.76 0 NaN 0<br />
5426 RB679_hypothetical protein 362787 362605 -1.54 0 NaN 0<br />
5462 RB6849_hypothetical protein 3642537 3642232 -1.48 0 0 0<br />
5467 RB6858_dehydrogenases and related proteins 3646807 3645782 -1.58 0 0 0<br />
5471 RB6864_hypothetical protein 3648295 3648429 -1.65 0 NaN 0<br />
5513 RB6938_membrane protein 3685985 3684477 -1.5 0 0 0<br />
5521 RB6958_conserved hypothetical protein 3695752 3695186 -1.55 0 0 0<br />
5536 RB6984_hypothetical protein 3717257 3717394 -1.46 0 NaN 0<br />
5589 RB7072_lipoate-protein ligase A 3754556 3753783 6.3.2.- -1.53 0 NaN 0<br />
5615 RB7109_hypothetical protein 3775707 3775546 -1.72 0 NaN 0<br />
5708 RB7246_hypothetical protein 3850393 3850545 -1.5 0 0 0<br />
5935 RB7628_hypothetical protein 4104029 4103661 -1.51 0 NaN 0<br />
5947 RB7646_hypothetical protein 4111735 4111923 -1.46 0 0 0<br />
6290 RB8223_protein containing DUF1559 4399759 4398527 -1.68 0 NaN 0<br />
6597 RB8749_hypothetical protein 4649927 4649739 -1.48 0 NaN 0<br />
6762 RB9034_conserved hypothetical protein, secreted 4818355 4821450 -1.54 0 NaN 0<br />
6814 RB9130_hypothetical protein 4886818 4886018 -1.48 0 0 0<br />
6868 RB9228_hypothetical protein 4934679 4936592 -1.51 0 NaN 0<br />
6964 RB9385_ribose transport ATP-binding protein rbsA 5033732 5032209 3.6.3.17 -1.56 0 NaN 0<br />
7248 RB9861_hypothetical protein 5313829 5314125 -1.47 0 NaN 0<br />
3820 RB4269_glutamic acid specific endopeptidase, GSE=serine endopeptidase 2185049 2184201 -1.61 0 -1.57 0<br />
5425 RB6789_secreted protein 3608487 3607945 -1.61 0 -1.53 0<br />
5574 RB7042_hypothetical protein 3743971 3744246 -1.63 0 -1.61 0
Cluster 13<br />
Original row UID Start Stop EC 62 h vs 44 h 82 h va 62 h 96 h vs 82 h 240 h vs 82 h<br />
7202 RB9786_hypothetical protein 5270645 5270205 0 0 -1.68 0<br />
126 RB10199_hypothetical protein 5512721 5512584 0 0 -2.24 -1.61<br />
395 RB10668_hypothetical protein 5796528 5796352 0 0 -1.95 0<br />
436 RB10734_conserved hypothetical protein, secreted 5833947 5834780 0 0 -1.6 0<br />
558 RB10937_hypothetical protein 5934561 5934376 0 0 -1.48 0<br />
703 RB11184_secreted protein 6068441 6068316 0 0 -1.51 0<br />
944 RB11589_conserved hypothetical protein, membrane 6251607 6250897 0 0 -1.47 0<br />
1139 RB11922_membrane protein containing DUF420 6419037 6418585 0 0 -1.53 0<br />
1619 RB12691_conserved hypothetical protein 6834264 6834085 0 0 -1.76 0<br />
2032 RB1395_secreted protein similar to DNA-binding protein 712097 713056 0 0 -2.33 0<br />
2428 RB2096_hypothetical protein 1106467 1105484 0 0 -1.49 0<br />
2554 RB2302_hypothetical protein 1204388 1204528 0 0 -1.46 0<br />
2828 RB2714_protein containing DUF541 1415817 1414909 0 0 -1.59 0<br />
2978 RB2958_hypothetical protein 1524723 1524962 0 0 -1.48 0<br />
3483 RB3739_conserved hypothetical protein 1935471 1935052 0 0 -1.64 0<br />
3578 RB3880_hypothetical protein 2006953 2006705 0 0 -1.64 0<br />
3608 RB3925_hypothetical protein 2033534 2033382 0 0 -1.57 0<br />
3625 RB3949_hypothetical protein 2042289 2042528 0 0 -1.98 0<br />
3643 RB3975_hypothetical protein 2056726 2057340 0 0 -1.54 0<br />
3646 RB3981_hypothetical protein 2058000 2058485 0 0 -1.86 0<br />
3903 RB4393_hypothetical protein 2261154 2261375 0 0 -1.51 0<br />
4084 RB4657_hypothetical protein 2381865 2382236 0 0 -1.84 0<br />
4242 RB4886_hypothetical protein 2513213 2513010 0 1.56 -1.74 0<br />
4493 RB5271_hypothetical protein 2717327 2717596 0 0 -1.78 0<br />
4535 RB5347_hypothetical protein 2764290 2764442 0 0 -2.27 0<br />
4671 RB5555_hypothetical protein 2891274 2891438 0 0 -1.78 0<br />
4691 RB5587_secreted protein 2908004 2907345 NaN 0 -1.52 0<br />
4882 RB59_hypothetical protein 25208 24939 0 0 -1.5 0<br />
5029 RB6158_hypothetical protein 3233532 3233104 0 0 -1.51 0<br />
5235 RB6489_low-specificity D-threonine aldolase 3435940 3434765 NaN 0 -1.46 0<br />
5303 RB6594_hypothetical protein 3505472 3506359 0 0 -1.82 0<br />
5393 RB6740_nonspecific acid phosphatase precursor 3579474 3580466 NaN 0 -1.58 0<br />
5680 RB7207_hypothetical protein 3822537 3822896 0 0 -1.71 0<br />
5843 RB7494_oxidoreductase protein 4019046 4020083 0 0 -1.61 0<br />
5867 RB7535_hypothetical protein 4050024 4050434 0 0 -1.94 0<br />
6676 RB8881_hypothetical protein 4726480 4726013 0 0 -1.48 0<br />
6815 RB9132_conserved hypothetical protein 4886727 4888004 0 0 -1.68 0<br />
6939 RB9346_hypothetical protein 5008335 5008550 0 0 -1.86 1.78<br />
7084 RB9578_hypothetical protein 5150392 5150592 0 0 -1.5 0<br />
1437 RB1240_protein containing DUF1080 634060 632546 0 0 -2.43 1.52<br />
1617 RB1269_hypothetical protein 649412 648801 0 0 -3.09 1.86<br />
2236 RB1751_hypothetical protein 910027 909821 0 0 -2.24 1.94<br />
2268 RB1814_hypothetical protein 939221 938487 0 0 -1.72 1.49
Cluster 14<br />
Original row UID Start Stop EC 62 h vs 44 h 82 h va 62 h 96 h vs 82 h 240 h vs 82 h<br />
7298 RB9945_secreted protein 5355360 5356313 0 -1.86 -3.42 0<br />
1370 RB1230_hypothetical protein 627328 626975 0 -1.46 -2.95 0<br />
1393 RB12329_conserved hypothetical protein, membrane 6634234 6634566 2.02 -1.72 -2.44 0<br />
1889 RB13148_arylsulfatase A [precursor] 7058006 7054968 0 -1.49 -2.64 1.48<br />
2103 RB1513_secreted protein 775203 774907 0 -1.49 -1.59 1.66<br />
3058 RB3074_hypothetical protein 1585325 1585164 0 -1.53 -1.99 0<br />
3093 RB3152_hypothetical protein 1626667 1626461 0 -1.8 -1.65 2.03<br />
3526 RB3802_hypothetical protein 1969456 1969262 0 -1.86 -2.09 0<br />
4131 RB4727_hypothetical protein 2422602 2422766 0 -1.8 -2.98 2<br />
4150 RB4750_membrane protein 2435462 2436076 0 -1.46 -2.71 1.53<br />
4512 RB5303_hypothetical protein 2736717 2736514 0 -1.73 -2.5 0<br />
4582 RB5415_hypothetical protein 2798737 2798925 0 -1.58 -1.52 0<br />
5049 RB6193_hypothetical protein 3246096 3245908 0 -2.27 -1.86 0<br />
6686 RB8895_Sialidase 4736087 4737343 3.2.1.18 0 -1.46 -2.02 0<br />
6754 RB9020_secreted protein 4807486 4808631 0 -2.13 -2.63 0
Cluster 16<br />
Original row UID Start Stop EC 62 h vs 44 h 82 h va 62 h 96 h vs 82 h 240 h vs 82 h<br />
83 RB10126_hypothetical protein 5465210 5465341 0 0 2.24 4.22<br />
6366 RB8353_hypothetical protein 4459374 4459622 NaN 0 2.43 1.83<br />
15 RB10022_membrane protein 5396916 5397413 NaN 0 NaN 1.61<br />
40 RB10053_secreted protein containing DUF1501 5423854 5425344 NaN 0 NaN 1.58<br />
42 RB10060_Bacterial regulatory protein, ArsR 5426810 5427139 0 0 NaN 1.46<br />
66 RB10105_protein containing DUF1582 5451202 5450855 NaN 0 NaN 1.53<br />
68 RB10107_hypothetical protein 5451876 5451724 NaN 0 NaN 1.63<br />
77 RB10119_serine/threonine protein kinase 5462723 5460423 2.7.1.37 NaN 0 NaN 1.63<br />
108 RB10167_hypothetical protein 5497096 5496917 NaN 0 NaN 1.68<br />
111 RB10172_aldehyde dehydrogenase family protein (Betaine) 5499799 5498330 1.2.1.36 NaN 0 NaN 1.51<br />
151 RB10238_protein containing DUF1559 5531834 5533567 NaN 0 NaN 1.72<br />
163 RB10263_hypothetical protein 5546338 5546162 NaN 0 2.07 1.49<br />
213 RB10347_hypothetical protein 5596258 5595704 0 0 NaN 1.69<br />
233 RB1039_Seryl-tRNA synthetase, class IIa 541472 542800 6.1.1.11 NaN 0 NaN 1.47<br />
251 RB10419_hypothetical protein 5639506 5639384 NaN 0 NaN 1.5<br />
254 RB10427_hypothetical protein 5647025 5646882 NaN 0 NaN 1.5<br />
259 RB10433_membrane protein 5651746 5650421 NaN 0 NaN 1.72<br />
470 RB10787_glycerate dehydrogenase/hydroxypyruvate reductase 5860031 5858568 1.1.1.81 NaN 0 NaN 1.45<br />
573 RB10965_conserved hypothetical protein, membrane 5942116 5942730 NaN 0 NaN 1.7<br />
581 RB10978_Small multidrug resistance protein 5947515 5947883 NaN 0 NaN 1.59<br />
607 RB11016_protein containing DUF1559 5974211 5973102 NaN 0 1.59 1.48<br />
615 RB11031_hypothetical protein 5980865 5981053 NaN 0 1.66 2.68<br />
630 RB11062_conserved hypothetical protein 5995358 5994714 NaN 0 NaN 1.69<br />
636 RB11070_hypothetical protein 6000321 6000479 0 0 1.71 2.84<br />
686 RB11156_secreted protein 6056074 6055022 NaN 0 NaN 1.84<br />
712 RB1120_multidrug efflux protein, outer membrane component 576988 575033 NaN 0 NaN 1.61<br />
754 RB11268_hypothetical protein 6109768 6109475 NaN 0 1.83 1.86<br />
778 RB11303_Transposase IS66 6128599 6127205 NaN 0 NaN 1.82<br />
783 RB11310_hypothetical protein 6130268 6130516 NaN 0 NaN 1.49<br />
813 RB11356_glycosyltransferase, family 2 6158199 6157486 NaN 0 NaN 1.62<br />
855 RB11424_conserved hypothetical protein 6195656 6196132 NaN 0 4.52 3.97<br />
856 RB11427_conserved hypothetical protein 6196136 6196417 NaN 0 2.45 2.67<br />
857 RB11429_conserved hypothetical protein 6196478 6196591 0 0 4.88 4.84<br />
864 RB11439_conserved hypothetical protein 6197993 6198274 0 0 4.95 4.77<br />
866 RB11446_conserved hypothetical protein 6199492 6199833 -1.45 0 2.4 4.37<br />
868 RB11450_conserved hypothetical protein 6200046 6200228 0 0 3.94 3.29<br />
874 RB11468_conserved hypothetical protein, secreted 6205637 6204918 NaN 0 NaN 1.6<br />
886 RB11484_conserved hypothetical protein 6212742 6212611 NaN 0 NaN 1.73<br />
989 RB11663_sugar transport protein 6290653 6292005 NaN 0 NaN 1.78
1004 RB11679_conserved hypothetical protein 6300368 6300523 NaN 0 NaN 1.46<br />
1006 RB11680_conserved hypothetical protein 6300635 6300456 NaN 0 NaN 1.9<br />
1010 RB11685_conserved hypothetical protein 6301256 6301077 NaN 0 NaN 1.62<br />
1017 RB117_membrane or secreted protein 51525 51136 NaN 0 NaN 1.85<br />
1030 RB11722_conserved hypothetical protein 6317037 6318065 NaN 0 NaN 1.76<br />
1059 RB11769_domain/calx-beta domain protein 6335006 6359527 NaN 0 NaN 1.55<br />
1066 RB1180_hypothetical protein 604378 604656 NaN 0 NaN 1.68<br />
1069 RB11803_ISxac3 transposase 6362243 6363121 0 0 NaN 1.48<br />
1081 RB11823_conserved hypothetical protein, secreted 6371378 6373282 NaN 0 NaN 1.94<br />
1082 RB11826_conserved hypothetical protein 6373298 6373600 0 0 NaN 1.66<br />
1090 RB1184_protein containing DUF1556 605971 605675 0 0 NaN 1.63<br />
1092 RB11844_conserved hypothetical protein 6378510 6378794 NaN 0 NaN 1.69<br />
1124 RB1190_integrase 607131 608009 NaN 0 NaN 1.53<br />
1137 RB1192_INTEGRASE/RECOMBINASE 609496 608450 0 0 NaN 1.47<br />
1172 RB11973_conserved hypothetical protein, membrane 6447543 6446722 NaN 0 NaN 1.52<br />
1197 RB12009_conserved hypothetical protein 6479495 6477951 NaN 0 NaN 1.69<br />
1259 RB12116_DNA polymerase III epsilon subunit 6541348 6540281 NaN 0 NaN 1.53<br />
1318 RB12219_conserved hypothetical protein 6586411 6584993 NaN 0 NaN 1.83<br />
1347 RB12265_conserved hypothetical protein 6602530 6602402 NaN 0 NaN 1.51<br />
1436 RB12398_conserved hypothetical protein 6672013 6670928 NaN 0 NaN 1.71<br />
1465 RB12442_conserved hypothetical protein 6698977 6697442 NaN 0 NaN 1.77<br />
1502 RB12500_flagellum-specific ATP synthase FliI 6730666 6729263 NaN 0 NaN 1.47<br />
1530 RB1255_hypothetical protein 640910 640755 NaN 0 NaN 1.97<br />
1597 RB1266_hypothetical protein 648804 648505 NaN 0 NaN 1.58<br />
1616 RB12689_secreted protein 6833004 6833324 NaN 0 2.18 2.12<br />
1618 RB12690_3-deoxy-manno-octulosonate cytidylyltransferase 6834088 6833336 2.7.7.38 NaN 0 NaN 1.93<br />
1622 RB12694_serine/threonine-protein kinase 6837178 6836147 2.7.1.37 0 0 NaN 1.79<br />
1631 RB12710_conserved hypothetical protein 6846593 6846703 NaN 0 NaN 1.73<br />
1642 RB12734_conserved hypothetical protein, secreted 6863889 6862186 NaN 0 NaN 1.49<br />
1683 RB12799_DNA-directed DNA polymerase 6908262 6905179 2.7.7.7 NaN 0 NaN 1.46<br />
1689 RB12806_conserved hypothetical protein 6911165 6910548 NaN 0 NaN 1.69<br />
1700 RB12820_conserved hypothetical protein 6916142 6915996 0 0 NaN 1.46<br />
1720 RB12851_conserved hypothetical protein 6926556 6926885 NaN 1.52 NaN 1.9<br />
1746 RB12891_LmbE-like protein 6944411 6945187 NaN 0 NaN 1.7<br />
1769 RB12941_integrase 6966539 6967417 NaN 0 NaN 1.48<br />
1787 RB12978_conserved hypothetical protein 6980722 6980057 NaN 0 NaN 1.52<br />
1818 RB13030_secreted protein containing DUF1559 6999186 6998161 NaN 0 NaN 1.49<br />
1822 RB13038_conserved hypothetical protein, membrane 7005061 7002800 NaN 0 1.46 2.21<br />
1847 RB13088_conserved hypothetical protein 7022309 7021677 0 0 NaN 1.93<br />
1902 RB13172_conserved hypothetical protein 7070198 7069962 NaN 0 NaN 1.94<br />
1908 RB13184_protein disulphide isomerase 7079314 7078691 0 0 NaN 1.56<br />
1929 RB13217_Penicillinase repressor 7091881 7091498 NaN 0 NaN 1.63
1930 RB1322_hypothetical protein 671526 671633 0 0 2.28 3.63<br />
1955 RB13259_conserved hypothetical protein 7108365 7106461 NaN 0 NaN 1.97<br />
1962 RB13267_conserved hypothetical protein 7117297 7116578 NaN 0 NaN 1.8<br />
1989 RB13323_conserved hypothetical protein 7141536 7141673 0 0 NaN 1.55<br />
1998 RB1339_hypothetical protein 681689 681384 NaN 0 NaN 1.7<br />
2007 RB1352_hypothetical protein 686463 687803 NaN 0 NaN 1.53<br />
2016 RB1362_hypothetical protein 696352 696600 0 0 1.85 3.65<br />
2017 RB1363_alanine dehydrogenase 697673 696588 1.4.1.1 NaN 0 NaN 1.49<br />
2025 RB1384_membrane protein 707461 706928 NaN 0 NaN 1.98<br />
2046 RB1420_inosine-uridine preferring nucleoside hydrolase 723375 724427 0 0 NaN 1.64<br />
2052 RB1432_NADH-ubiquinone oxidoreductase, chain 4L 732414 731815 1.6.5.3 NaN 0 NaN 1.71<br />
2057 RB144_conserved hypothetical protein 68273 66870 NaN 0 NaN 1.71<br />
2116 RB1531_membrane protein 782534 783889 NaN 0 NaN 1.77<br />
2132 RB1563_hypothetical protein 798934 799344 NaN 0 2.09 4.12<br />
2158 RB1604_DNA alkylation repair enzyme 826373 825174 0 0 NaN 1.65<br />
2169 RB1624_hypothetical protein 835745 835452 NaN 0 NaN 1.76<br />
2178 RB1638_hypothetical protein 842521 842718 NaN 0 NaN 1.83<br />
2180 RB164_hypothetical protein 87060 87560 NaN 0 NaN 2<br />
2184 RB1648_secreted protein 846423 846118 NaN 0 NaN 1.9<br />
2203 RB17_protein containing DUF1589 8251 7757 NaN 0 NaN 1.86<br />
2216 RB1717_conserved hypothetical protein, membrane 897501 895876 0 0 NaN 1.69<br />
2235 RB1750_hypothetical protein 909661 909837 NaN 0 NaN 1.75<br />
2246 RB1771_hypothetical protein 919524 919273 0 0 NaN 1.87<br />
2254 RB1785_hypothetical protein 925466 925621 NaN 0 NaN 1.46<br />
2295 RB1862_transcriptional regulator, GntR family 960630 961037 NaN 0 NaN 1.75<br />
2297 RB1868_membrane protein 962128 964635 0 0 NaN 2<br />
2313 RB1893_Serine/threonine-protein kinase 979639 982911 2.7.1.37 0 0 NaN 1.7<br />
2320 RB1901_hypothetical protein 988429 988833 0 0 NaN 1.93<br />
2379 RB2011_hypothetical protein 1059411 1059692 0 0 2.47 2.33<br />
2396 RB2038_hypothetical protein 1076265 1071604 0 0 NaN 1.84<br />
2424 RB2088_secreted protein 1100666 1101931 NaN 0 NaN 1.5<br />
2463 RB2155_hypothetical protein 1140660 1140190 NaN 0 NaN 1.99<br />
2521 RB2254_choline sulfatase 1184068 1182443 3.1.6.6 NaN 0 NaN 1.81<br />
2528 RB2262_hypothetical protein 1188965 1188777 NaN 0 NaN 1.88<br />
2545 RB2287_protein containing DUF1586 1199020 1198829 NaN 0 NaN 1.75<br />
2549 RB2291_Sporulation initiation inhibitor protein soj 1199317 1200063 NaN 0 NaN 1.46<br />
2582 RB2342_hypothetical protein 1217901 1218119 NaN 0 NaN 1.57<br />
2613 RB2382_hypothetical protein 1238921 1239148 0 0 1.61 1.8<br />
2685 RB2492_hypothetical protein 1302190 1302399 0 0 2.12 3.1<br />
2704 RB2510_hypothetical protein 1313396 1313563 NaN 0 NaN 1.69<br />
2710 RB2516_hypothetical protein 1316669 1316860 0 0 1.76 2.3<br />
2745 RB2574_hypothetical protein 1342282 1341533 NaN 0 NaN 1.57
2779 RB2636_hypothetical protein 1368450 1368100 0 0 2.94 2.49<br />
2804 RB2679_hypothetical protein 1391557 1392078 0 0 1.53 2<br />
2873 RB2795_hypothetical protein 1454726 1455370 NaN 0 NaN 1.53<br />
2886 RB2818_hypothetical protein 1463057 1462893 NaN 0 NaN 1.86<br />
2914 RB286_hypothetical protein 142779 143096 0 0 NaN 1.87<br />
2925 RB2878_conserved hypothetical protein 1497670 1498329 NaN 0 NaN 1.5<br />
2945 RB2912_secreted protein 1512238 1511297 NaN 0 NaN 1.97<br />
3086 RB3144_INTEGRASE/RECOMBINASE 1621739 1622785 NaN 0 NaN 1.58<br />
3096 RB3155_hypothetical protein 1627430 1627149 NaN 0 NaN 1.74<br />
3201 RB3326_hypothetical protein 1711353 1711640 NaN 0 NaN 1.95<br />
3326 RB3509_glycosyl hydrolase 1818015 1819025 NaN 0 NaN 1.52<br />
3765 RB4185_hypothetical protein 2151951 2152220 0 0 1.51 2.38<br />
3870 RB4341_hypothetical protein 2234849 2235178 NaN 0 NaN 1.66<br />
3907 RB4397_protein containing DUF1560 2263976 2264143 0 0 2.12 2.69<br />
3960 RB4473_hypothetical protein 2294981 2294808 NaN 0 NaN 1.51<br />
3987 RB4510_hypothetical protein 2307657 2307508 NaN 0 1.6 3.28<br />
4018 RB4558_hypothetical protein 2333224 2332976 NaN 0 NaN 1.46<br />
4041 RB4598_membrane protein 2355806 2356726 NaN 0 NaN 1.58<br />
4075 RB4645_hypothetical protein 2378266 2378057 0 0 NaN 1.64<br />
4197 RB4815_arylsulfatase precursor 2469289 2467814 3.1.6.8 0 0 NaN 1.87<br />
4202 RB4826_Transposase IS66 2471205 2472599 NaN 0 NaN 1.66<br />
4247 RB4894_glycogen debranching enzyme 2518939 2516801 3.2.1.- NaN 0 NaN 1.64<br />
4380 RB5096_hypothetical protein 2619419 2619643 0 0 NaN 1.47<br />
4422 RB5164_hypothetical protein 2658365 2657709 0 0 3 2.56<br />
4454 RB521_hypothetical protein 286399 286208 0 0 2.57 4.51<br />
4580 RB5412_hypothetical protein 2794838 2794620 NaN 0 1.69 3.52<br />
4674 RB556_hypothetical protein 308213 308356 NaN 0 NaN 1.71<br />
4681 RB557_hypothetical protein 308223 308384 NaN 0 NaN 1.8<br />
4763 RB5707_hypothetical protein 2979435 2979986 -1.47 0 2.8 3.49<br />
4782 RB5737_hypothetical protein 2994415 2994777 0 0 1.56 2.17<br />
4859 RB5867_hypothetical protein 3077578 3077730 NaN 0 NaN 1.64<br />
4890 RB5914_hypothetical protein 3097264 3097434 0 0 3.77 2.91<br />
4949 RB6011_hypothetical protein 3150235 3150083 NaN 0 NaN 1.68<br />
4953 RB6016_hypothetical protein 3152860 3153426 NaN 0 NaN 1.91<br />
5001 RB6108_secreted protein 3208185 3204895 NaN 0 NaN 1.52<br />
5034 RB6167_Putative transposase 3235937 3237064 NaN 0 NaN 1.5<br />
5084 RB6250_hypothetical protein 3282079 3282186 NaN 0 NaN 1.51<br />
5145 RB6355_iron-sulfur cluster-binding protein 3336973 3338490 0 0 NaN 1.53<br />
5217 RB6459_cell surface protein 3408788 3414874 NaN 0 NaN 1.59<br />
5288 RB6571_secreted protein 3489550 3490014 0 0 NaN 1.63<br />
5307 RB66_hypothetical protein 26082 26336 0 0 NaN 1.62<br />
5323 RB663_hypothetical protein 357608 357369 0 0 2.72 2.08
5328 RB6637_hypothetical protein 3528317 3528469 NaN 0 NaN 1.5<br />
5335 RB6655_hypothetical protein 3535829 3535719 NaN 0 NaN 1.58<br />
5354 RB6684_hypothetical protein 3554034 3553717 0 0 2.45 1.61<br />
5355 RB6685_hypothetical protein 3554265 3554047 NaN 0 NaN 1.6<br />
5369 RB6706_hypothetical protein 3560942 3560829 NaN 0 NaN 1.93<br />
5389 RB6735_hypothetical protein 3577540 3577388 NaN 0 NaN 1.48<br />
5488 RB6903_hypothetical protein 3669220 3669059 0 0 NaN 1.64<br />
5505 RB6925_hypothetical protein 3678461 3677976 3.02 -1.91 2.05 3.78<br />
5511 RB6935_pyruvate:ferredoxin oxidoreductase (alpha subunit) 3684260 3682377 NaN 0 NaN 1.8<br />
5549 RB7002_secreted protein 3725925 3726623 NaN 0 NaN 1.96<br />
5565 RB7028_hypothetical protein 3736857 3738494 NaN 0 NaN 1.6<br />
5622 RB7116_hypothetical protein 3779586 3779765 0 0 1.48 3.59<br />
5697 RB723_hypothetical protein 388051 388233 NaN 0 1.78 2.62<br />
5777 RB7388_INTEGRASE/RECOMBINASE 3952745 3953593 NaN 0 NaN 1.8<br />
5781 RB7393_hypothetical protein 3955353 3955553 0 0 NaN 1.77<br />
5826 RB7464_hypothetical protein 4001614 4001480 0 0 2.38 3.26<br />
5830 RB7471_hypothetical protein 4006361 4006083 NaN 0 NaN 1.77<br />
5839 RB7486_hypothetical protein 4017064 4016672 NaN 0 NaN 1.84<br />
5951 RB7651_hypothetical protein 4112517 4112383 NaN 0 NaN 1.91<br />
6069 RB7833_Ribosomal protein L3 4192692 4193378 NaN 0 2.41 1.69<br />
6112 RB7909_secreted protein 4225494 4224502 NaN 0 NaN 1.67<br />
6122 RB7924_RNA polymerase sigma factor 4231813 4232499 NaN 0 NaN 1.69<br />
6199 RB8065_hypothetical protein 4318540 4318424 0 0 NaN 2<br />
6306 RB8248_dienelactone hydrolase family protein 4409395 4410156 NaN 0 NaN 1.58<br />
6385 RB8384_ISXo8 transposase 4478623 4479924 NaN 0 NaN 1.75<br />
6390 RB839_hypothetical protein 441662 441844 NaN 0 2.22 2.18<br />
6397 RB8404_Silent information regulator protein Sir2 4492899 4493618 NaN 0 NaN 1.71<br />
6425 RB8457_hypothetical protein 4518458 4518117 0 0 2.14 3.16<br />
6435 RB8474_hypothetical protein 4524096 4523554 0 0 NaN 1.47<br />
6437 RB8478_hypothetical protein 4524759 4524334 NaN 0 NaN 1.74<br />
6481 RB8541_Peptidase M42 4554758 4555831 NaN 0 NaN 1.64<br />
6525 RB862_hypothetical protein 457775 457945 NaN 0 NaN 1.5<br />
6554 RB8681_hypothetical protein 4621513 4621959 NaN 0 NaN 1.48<br />
6581 RB8726_single-stranded-DNA-specific exonuclease RecJ 4638505 4640304 3.1.-.- NaN 0 NaN 1.57<br />
6634 RB8815_hypothetical protein 4687955 4687740 0 0 2.55 3.22<br />
6667 RB8866_secreted conserved hypothetical protein 4716964 4718301 0 0 NaN 1.45<br />
6736 RB8980_protein containing DUF1559 4788689 4786833 NaN 0 NaN 1.74<br />
6739 RB8987_hypothetical protein 4790856 4790710 NaN 0 NaN 1.64<br />
6752 RB9017_hypthetical protein 4805225 4805806 NaN 0 NaN 1.68<br />
6757 RB9024_hypothetical protein 4809190 4809050 NaN 0 NaN 1.65<br />
6822 RB9144_hypothetical protein 4893346 4893798 NaN 0 NaN 2<br />
6844 RB919_hypothetical protein 488799 488470 NaN 0 NaN 1.77
6845 RB9190_ferredoxin 4914039 4914437 NaN 0 NaN 2.01<br />
6883 RB9249_hypothetical protein 4947571 4945139 NaN 0 NaN 1.66<br />
6915 RB9308_Outer membrane efflux family protein 4978849 4976960 NaN 0 NaN 2.02<br />
6932 RB9338_formaldehyde-activating enzyme 5002305 5002853 0 0 NaN 1.51<br />
6988 RB9423_hypothetical protein 5056635 5057219 0 0 NaN 1.48<br />
7079 RB957_Tyrosine recombinase xerD 505476 504889 NaN 0 NaN 1.98<br />
7092 RB9592_conserved hypothetical protein 5157928 5158737 NaN 0 NaN 1.55<br />
7162 RB971_Patatin-like phospholipase 509411 508101 0 0 NaN 1.47<br />
7208 RB9793_hypothetical protein 5273041 5272850 NaN 0 NaN 1.85<br />
7276 RB9907_ISXo8 transposase 5338185 5339486 NaN 0 NaN 1.93<br />
9 RB10014_protein containing DUF1552 5395577 5393973 0 0 NaN 2.04<br />
60 RB10098_hypothetical protein 5447310 5447453 0 0 NaN 1.89<br />
362 RB10612_choline-sulfatase 5761454 5762932 3.1.6.6 NaN 0 NaN 1.89<br />
449 RB10752_hypothetical protein 5838740 5838612 NaN 0 NaN 1.9<br />
456 RB10764_hypothetical protein 5845271 5844684 0 0 NaN 1.99<br />
484 RB10816_DNA polymerase III tau and gamma subunits 5875071 5873941 0 0 NaN 1.86<br />
506 RB10855_mandelate racemase/muconate lactonizing enzyme family protein 5895378 5894191 0 0 NaN 1.99<br />
518 RB10876_excinuclease ABC subunit A 5903770 5901251 NaN 0 NaN 1.85<br />
610 RB11023_hypothetical protein 5975603 5975424 NaN 0 NaN 1.81<br />
732 RB11228_hypothetical protein 6087599 6087471 0 0 NaN 2<br />
958 RB11616_conserved hypothetical protein 6262622 6262452 NaN 0 NaN 2.05<br />
1046 RB11750_integrase 6328357 6329235 NaN 0 NaN 2.07<br />
1336 RB12243_conserved hypothetical protein 6596256 6596423 NaN 0 NaN 2.01<br />
1419 RB12377_Ribosomal RNA adenine methylase transferase 6661963 6661109 NaN 0 1.49 4.03<br />
2997 RB2983_Hydrogen peroxide-inducible genes activator 1540680 1539799 0 0 NaN 2.05<br />
4441 RB519_hypothetical protein 285036 284872 NaN 0 NaN 2.07<br />
4875 RB5887_integrase 3087343 3086465 0 0 NaN 2.05
Cluster 17<br />
Original row UID Start Stop EC 62 h vs 44 h 82 h va 62 h 96 h vs 82 h 240 h vs 82 h<br />
4122 RB4715_conserved hypothetical protein 2413294 2417169 0 -1.52 0 0<br />
568 RB10954_hypothetical protein 5940749 5940318 NaN -1.83 0 0<br />
3228 RB3365_hypothetical protein 1730124 1730255 0 -1.85 0 0<br />
4196 RB4814_hypothetical protein 2467537 2467800 0 -1.51 0 0<br />
4810 RB5779_hypothetical protein 3028410 3028973 0 -1.83 0 0<br />
6221 RB8098_hypothetical protein 4337334 4337119 0 -1.95 0 0<br />
6831 RB9160_hypothetical protein 4900491 4900847 0 -1.46 0 0<br />
7025 RB9493_acetyltransferase 5096754 5097221 0 -1.5 0 0<br />
4036 RB4590_hypothetical protein 2352024 2351800 1.51 -2.24 0 0<br />
514 RB10868_hypothetical protein 5900330 5899920 0 -2.12 0 2.01
Cluster 18<br />
Original row UID Start Stop EC 62 h vs 44 h 82 h va 62 h 96 h vs 82 h 240 h vs 82 h<br />
2096 RB1501_hypothetical protein 769419 769571 NaN 1.65 2.68 0<br />
2618 RB2394_conserved hypothetical protein 1245630 1244920 NaN 0 3.13 0<br />
2866 RB2782_protein containing DUF1559 1445576 1447345 NaN 0 3.32 0<br />
4326 RB5003_Rh-like protein/ammonium transporter 2572662 2574158 NaN 0 3.11 0<br />
6469 RB8527_protein containing DUF1559 4549097 4550089 NaN 0 2.9 0<br />
601 RB11008_secreted protein 5967078 5968262 NaN 0 3.55 0<br />
5414 RB6777_hypothetical protein 3602786 3603199 NaN 0 3.04 NaN
Cluster 19<br />
Original row UID Start Stop EC 62 h vs 44 h 82 h va 62 h 96 h vs 82 h 240 h vs 82 h<br />
85 RB10129_FKBP-type peptidyl-prolyl cis-trans isomerase 5467429 5466713 5.2.1.8 NaN 1.54 NaN NaN<br />
3583 RB3886_Ribosome recycling factor 2008765 2009367 NaN 1.47 NaN NaN<br />
4144 RB4742_Universal stress protein 2433589 2432615 NaN 1.6 NaN NaN<br />
4322 RB5_secreted protein 1514 399 NaN 1.71 NaN NaN<br />
4344 RB5036_hypothetical protein 2590789 2590959 NaN 1.54 NaN NaN
Cluster 20<br />
Original row UID Start Stop EC 62 h vs 44 h 82 h va 62 h 96 h vs 82 h 240 h vs 82 h<br />
555 RB10932_secreted protein 5933872 5934069 NaN 0 3.11 -3.82<br />
578 RB10971_Short-chain dehydrogenase/reductase SDR 5946406 5945495 NaN 0 2.81 -1.74<br />
3961 RB4474_CsbD-like 2295048 2295554 NaN 0 3.76 -4.23<br />
5161 RB6379_hypothetical protein 3353122 3352661 NaN 0 3.01 -3.06<br />
6 RB10007_conserved hypothetical protein 5390130 5391071 NaN 1.65 2.48 -2.95<br />
162 RB10261_Carbon storage regulator 5545964 5545719 NaN 0 3.21 -4.16<br />
430 RB10727_manganese-containing catalase 5830775 5831605 NaN 2.64 3.91 -2.97<br />
509 RB10861_hypothetical protein 5896851 5896991 0 0 3.6 -3.48<br />
571 RB10958_hypothetical protein 5941015 5941491 NaN 0 3.41 -1.76<br />
837 RB11396_conserved hypothetical protein 6178445 6178555 0 0 1.5 -1.51<br />
838 RB11397_SOUL heme-binding protein 6178540 6179163 NaN 0 3.07 -2.5<br />
839 RB11399_conserved hypothetical protein 6179167 6179565 NaN 0 2.49 -1.49<br />
1284 RB12160_thioredoxin 6563104 6562778 0 0 2.53 -4.18<br />
1358 RB12283_conserved hypothetical protein 6609220 6609378 NaN 0 2.92 -3.41<br />
1368 RB12297_conserved hypothetical protein 6615598 6616107 NaN 0 2.4 -2.8<br />
1871 RB13117_nitrogen regulation protein 7036595 7037065 NaN 0 1.68 -2.21<br />
1954 RB13257_2-hydroxy-6-ketonona-2,4-dienedioic acid hydrolase 7105590 7106450 3.7.1.- NaN 0 2.81 -2<br />
2182 RB1644_secreted protein 845824 845453 NaN 0 3.27 -4.9<br />
2221 RB1728_membrane protein containing DUF1206 899954 900811 NaN 1.46 2.29 -2.53<br />
2806 RB2680_conserved hypothetical protein, membrane 1393566 1392163 NaN 0 2.91 -1.73<br />
3811 RB4256_conserved hypothetical protein, secreted 2180303 2181379 NaN 0 3.13 -1.7<br />
3911 RB4400_hypothetical protein 2265382 2265206 NaN 0 2.51 -3.08<br />
3932 RB4432_glucose 1-dehydrogenase or 3-oxoacyl-[acyl-carrier-protein] reductase 2276333 2277139 1.1.1.- NaN 0 2.69 -2.02<br />
3958 RB4468_hypothetical protein 2294104 2293616 NaN 0 2.36 -2.27<br />
3959 RB4470_secreted protein 2294676 2294167 0 0 2.85 -3.66<br />
3962 RB4476_hypothetical protein 2295557 2295943 NaN 0 3.1 -2.19<br />
3963 RB4477_hypothetical protein 2295940 2296149 NaN 0 2.7 -2.47<br />
3966 RB4480_hypothetical protein 2296646 2296915 NaN 0 1.85 -2.49<br />
3973 RB4492_hypothetical protein 2302138 2301833 0 0 2.31 -1.81<br />
3975 RB4497_hypothetical protein 2302950 2303099 0 0 3.12 -2.78<br />
3981 RB4502_Protein of unknown function, UPF0057 2303992 2304171 NaN 0 1.53 -1.59<br />
4325 RB5001_hypothetical protein 2572354 2572665 NaN 0 3.33 -1.65<br />
4444 RB5196_trehalose synthase 2678058 2675953 5.4.99.16 NaN 0 2.39 -1.85<br />
4850 RB5850_hypothetical protein 3066498 3066307 NaN 0 2.37 -2.11<br />
5042 RB6178_hypothetical protein 3240162 3240287 NaN 0 2.15 -1.84<br />
5194 RB6428_conserved hypothetical protein, secreted 3382816 3382322 NaN 0 3.39 -1.53<br />
5195 RB6429_hypothetical protein 3382998 3382813 NaN 0 3.74 -2.59<br />
5498 RB6912_secreted protein 3672793 3672960 NaN 0 3.15 -1.8<br />
5749 RB7313_hypothetical protein 3892097 3891942 NaN 0 2.26 -2.52
5948 RB7647_hypothetical protein 4111931 4112152 NaN 0 4.19 -5.22<br />
6245 RB8143_hypothetical protein 4354179 4353961 NaN 0 1.79 -1.49<br />
6854 RB9200_4-nitrophenylphosphatase 4919220 4918369 3.1.3.41 0 0 2.83 -1.59<br />
908 RB11516_HD domain protein 6225331 6224708 NaN 0 NaN -1.47<br />
1772 RB12946_conserved hypothetical protein 6969668 6970402 NaN 0 NaN -1.45<br />
2298 RB187_hypothetical protein 95474 95367 NaN 0 NaN -1.46<br />
2726 RB2543_30S ribosomal protein S1 1326685 1328760 NaN 0 NaN -1.52<br />
3009 RB3_hypothetical protein 39 260 NaN 0 NaN -1.51<br />
3125 RB3205_hypothetical protein 1652079 1651849 NaN 0 NaN -1.63<br />
3156 RB3257_hypothetical protein 1677159 1677374 NaN 0 NaN -1.48<br />
3250 RB3399_hypothetical protein 1753650 1753453 0 0 NaN -1.65<br />
3384 RB3603_secreted protein 1875461 1874427 0 0 NaN -1.52<br />
3606 RB3923_hypothetical protein 2032453 2032286 NaN 0 NaN -1.62<br />
3651 RB3989_membrane protein 2060382 2060714 0 0 NaN -1.57<br />
3657 RB3994_hypothetical protein 2061291 2061866 NaN 0 NaN -1.62<br />
3727 RB4116_conserved hypothetical protein, membrane 2122532 2119149 0 0 NaN -1.47<br />
3807 RB4250_hypothetical protein 2179578 2179745 NaN 0 NaN -1.49<br />
3844 RB430_protein containing DUF1501 229809 231377 NaN 0 NaN -1.51<br />
3882 RB4360_hypothetical protein 2240075 2239605 0 0 NaN -1.74<br />
3908 RB4398_hypothetical protein 2264261 2264154 0 0 NaN -1.72<br />
3954 RB4464_secreted protein 2292075 2291404 NaN 0 1.87 -3.33<br />
4154 RB4756_conserved hypothetical protein 2437784 2438053 NaN 0 NaN -1.46<br />
4174 RB479_secreted protein 259174 255869 NaN 0 NaN -1.76<br />
4256 RB4908_ATP synthase epsilon chain 2525588 2525983 3.6.3.14 NaN 0 NaN -1.46<br />
4274 RB4931_secreted protein 2537481 2538134 NaN 0 NaN -1.67<br />
4277 RB4936_O-acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine O-acyltransferase 2539280 2540089 2.3.1.129 NaN 0 NaN -1.74<br />
4297 RB497_methanol dehydrogenase regulatory protein; (moxR) 269443 270549 NaN 0 NaN -1.57<br />
4361 RB5064_secreted protein containing DUF1585 2605707 2607653 NaN 0 NaN -1.74<br />
4409 RB514_hypothetical protein 284567 284397 NaN 0 1.54 -2.44<br />
4439 RB5186_GTP-binding protein YchF 2671136 2669970 NaN 0 NaN -1.48<br />
4486 RB5261_hypothetical protein 2713712 2713332 NaN 0 NaN -1.78<br />
4501 RB5282_choline-sulfatase 2727443 2725707 3.1.6.6 NaN 0 NaN -1.56<br />
4553 RB5373_hypothetical protein 2775404 2775270 NaN 0 NaN -1.61<br />
4583 RB5416_RNA polymerase, alpha subunit 2798945 2803234 2.7.7.6 NaN 0 NaN -1.49<br />
4637 RB5499_conserved hypothetical protein containing pectin lyase fold and parallel beta-helix repeats 2844998 2843655 NaN 0 NaN -1.72<br />
4645 RB5506_hypothetical protein 2851102 2850947 NaN 0 NaN -1.76<br />
4669 RB5551_hypothetical protein 2889101 2891092 NaN 0 NaN -1.73<br />
4706 RB5613_protein containing DUF1006 2921546 2922628 NaN 0 NaN -1.72<br />
4749 RB5681_Trigger factor 2965080 2966843 NaN 0 NaN -1.9<br />
4767 RB5715_hypothetical protein 2982826 2983458 NaN 0 NaN -1.85<br />
4811 RB578_hypothetical protein 320230 320069 NaN 0 NaN -1.49<br />
4826 RB5804_polynucleotide phosphorylase 3040160 3042616 NaN 0 NaN -1.67
4895 RB5924_hypothetical protein 3105828 3103285 0 0 NaN -1.59<br />
4928 RB5983_Acetylglutamate kinase 3137623 3138486 2.7.2.8 0 0 NaN -1.47<br />
4981 RB6071_hypothetical protein 3187463 3188104 NaN 0 NaN -1.66<br />
5028 RB6156_membrane protein 3233026 3232622 NaN 0 NaN -1.53<br />
5162 RB638_PQQ enzyme repeat domain protein 346447 347757 NaN 0 NaN -1.81<br />
5249 RB6504_hypothetical protein 3456120 3455959 NaN 0 NaN -1.75<br />
5293 RB6578_secreted protein 3495957 3495022 NaN 0 NaN -1.5<br />
5366 RB6701_hypothetical protein 3559944 3560207 NaN 0 NaN -1.63<br />
5377 RB6716_arsenical pump membrane protein 3567209 3565803 NaN 0 NaN -1.46<br />
5383 RB6728_hypothetical protein 3570581 3570922 0 0 NaN -1.55<br />
5392 RB674_membrane protein 359779 359528 0 0 NaN -1.46<br />
5417 RB6780_RNA polymerase sigma factor rpoD 3603381 3605054 NaN 0 NaN -1.46<br />
5456 RB684_Arylsulfatase precursor 368557 366596 3.1.6.1 0 0 NaN -1.54<br />
5466 RB6856_hypothetical protein 3645613 3645425 NaN 0 NaN -1.57<br />
5484 RB6891_O-antigen polymerase-like protein, membrane 3665156 3662541 0 0 NaN -1.45<br />
5497 RB6911_protein containing DUF1559 3671445 3672737 NaN 0 2.09 -3.19<br />
5507 RB693_protein containing DUF1568 371190 370408 NaN 0 NaN -1.45<br />
5561 RB7022_Ribosomal protein S20 3735291 3735563 NaN 0 NaN -1.8<br />
5592 RB7078_outer membrane efflux protein 3758295 3760328 NaN 0 NaN -1.47<br />
5629 RB7129_iron-dependent transcriptional repressor 3782388 3782618 NaN 0 NaN -2.3<br />
5725 RB7279_hypothetical protein 3870019 3870309 NaN 0 NaN -2.04<br />
5737 RB7296_hypothetical protein 3877224 3877472 NaN 0 NaN -1.66<br />
5741 RB73_membrane protein 28727 29023 NaN 0 NaN -1.56<br />
5748 RB7312_hypothetical protein 3891868 3891704 NaN 0 NaN -1.65<br />
5849 RB7507_conserved hypothetical protein, membrane 4036748 4038313 NaN 0 NaN -1.6<br />
5899 RB7579_sigma-54 dependent transcriptional regulator/response regulator 4071162 4072619 NaN 0 NaN -1.49<br />
5900 RB758_hypothetical protein 407558 407340 NaN 0 NaN -1.51<br />
5908 RB7589_hypothetical protein 4080238 4079978 NaN 0 NaN -1.66<br />
5941 RB7634_Abortive infection protein 4106267 4108588 NaN 0 NaN -1.66<br />
5960 RB7667_hypothetical protein 4117097 4116981 NaN 0 1.75 -2.59<br />
5964 RB7673_membrane protein containing DUF107 4120808 4119234 NaN 0 NaN -1.48<br />
5993 RB7722_membrane protein 4137251 4136415 0 0 NaN -1.67<br />
6117 RB7914_hypothetical protein 4228476 4227877 NaN 0 NaN -1.52<br />
6128 RB7937_hypothetical protein 4237809 4237684 NaN 0 NaN -1.53<br />
6140 RB7955_hypothetical protein 4247114 4246743 NaN 0 NaN -1.5<br />
6150 RB7978_hypothetical protein 4265473 4265817 NaN 0 NaN -1.5<br />
6169 RB8012_hypothetical protein 4282669 4282280 NaN 0 NaN -1.53<br />
6176 RB8027_Sigma factor, ECF-like 4289305 4290003 NaN 0 NaN -1.51<br />
6181 RB8031_6-phosphogluconolactonase 4295373 4294183 3.1.1.31 NaN 0 NaN -2.2<br />
6182 RB8034_membrane protein 4295344 4295787 NaN 0 NaN -1.59<br />
6200 RB8066_hypothetical protein 4318635 4318519 NaN 0 NaN -1.51<br />
6204 RB8070_hypothetical protein 4319538 4319717 NaN 0 NaN -1.48
6246 RB8144_hypothetical protein 4354622 4354191 NaN 0 3.31 -6.02<br />
6248 RB8149_Carbon storage regulator 4355339 4355551 NaN 0 NaN -1.93<br />
6291 RB8226_hypothetical protein 4400097 4399783 NaN 0 NaN -1.68<br />
6292 RB8227_hypothetical protein 4400365 4400186 0 0 NaN -1.9<br />
6308 RB8253_ASPARTYL-TRNA SYNTHETASE ASPS (ASPARTATE--TRNA 4410353 4412131 6.1.1.12 NaN 0 NaN -1.68<br />
6309 RB8254_amino acid transporter, permease 4412322 4414511 NaN 0 NaN -1.66<br />
6357 RB8337_hypothetical protein 4454547 4452448 NaN 0 NaN -1.52<br />
6359 RB8342_hypothetical protein 4454696 4454565 NaN 0 NaN -1.57<br />
6368 RB8356_hypothetical protein 4459802 4460011 NaN 0 NaN -1.73<br />
6389 RB8389_membrane protein 4484734 4481915 NaN 0 NaN -1.48<br />
6434 RB8473_hypothetical protein 4523410 4523535 0 0 NaN -1.61<br />
6520 RB861_conserved hypothetical protein, membrane 457825 456326 NaN 0 NaN -1.56<br />
6561 RB8690_membrane protein 4628703 4626859 NaN 0 NaN -1.46<br />
6580 RB8725_50S ribosomal protein L33 4638301 4638465 NaN 0 NaN -1.73<br />
6616 RB8783_Endonuclease/exonuclease/phosphatase 4671168 4670203 NaN 0 NaN -1.49<br />
6626 RB88_phosphodiesterase/alkaline phosphatase 34254 35666 NaN 0 NaN -1.76<br />
6630 RB8807_membrane protein containing DUF502 4686313 4685078 NaN 0 NaN -1.62<br />
6702 RB8921_conserved hypothetical protein, membrane 4751930 4750749 NaN 0 NaN -1.57<br />
6725 RB8966_Chaperonin Cpn60/TCP-1 4777493 4779475 NaN 2.04 2.16 -2.92<br />
6726 RB8969_Chaperonin Cpn10 4779554 4779859 NaN 0 NaN -1.55<br />
6748 RB9011_secreted protein 4800960 4802027 NaN 0 NaN -1.54<br />
6811 RB9126_hypothetical protein 4885253 4885417 NaN 0 NaN -1.85<br />
6867 RB9227_hypothetical protein 4934649 4934332 NaN 0 NaN -1.72<br />
6911 RB9304_Ribosomal protein L31 4974378 4974623 NaN 0 1.47 -3.29<br />
6925 RB9324_secreted protein 4992474 4993277 NaN 0 NaN -1.67<br />
6937 RB9344_probable swi/snf family helicase 2 5003979 5008139 NaN 0 NaN -1.63<br />
7004 RB9448_conserved hypothetical membrane protein 5076267 5076614 0 0 NaN -1.69<br />
7005 RB945_L-lactate permease 500020 498338 NaN 0 NaN -1.48<br />
7019 RB9484_conserved hypothetical protein 5094011 5092452 NaN 0 NaN -1.51<br />
7045 RB9520_conserved hypothetical protein 5114495 5112897 NaN 0 NaN -1.64<br />
7082 RB9574_Short-chain dehydrogenase/reductase SDR 5150355 5149549 1.-.-.- NaN 0 NaN -1.63<br />
7085 RB9581_protein containing DUF24 5150921 5150535 NaN 0 NaN -2.39<br />
7090 RB9588_conserved hypothetical protein 5156821 5156015 NaN 0 NaN -1.84<br />
7099 RB9610_NAD-dependent epimerase/dehydratase 5169123 5170628 NaN 0 NaN -1.71<br />
7131 RB9657_OXIDOREDUCTASE PROTEIN 5202179 5203258 NaN 0 NaN -1.52<br />
7155 RB9696_Protein of unknown function DUF879, bacterial 5223290 5225191 0 0 NaN -1.83<br />
7164 RB9713_large, multifunctional secreted protein - Streptomyces coelicolor (fragment) 5243297 5240049 NaN 0 NaN -1.5<br />
7181 RB9745_serine/threonine protein kinase 5252442 5254412 2.7.1.37 NaN 0 NaN -1.6<br />
7192 RB9767_hypothetical protein 5264348 5264641 NaN 0 NaN -1.56<br />
7217 RB9806_membrane protein 5280590 5281087 0 0 NaN -1.76<br />
7230 RB9830_hypothetical protein 5293341 5293111 NaN 0 NaN -2.03<br />
7272 RB9902_secreted protein 5336299 5335763 NaN 0 NaN -1.62
7284 RB9920_Ribosomal protein S6 5344827 5344405 NaN 0 NaN -1.52<br />
7 RB10009_similar to cold shock-like protein cspI 5391076 5391459 NaN 0 NaN -1.97<br />
115 RB10178_hypothetical protein 5502418 5502570 NaN 0 NaN -1.73<br />
120 RB10184_hypothetical protein 5507891 5507610 NaN 0 NaN -1.59<br />
208 RB10342_hypothetical protein 5593929 5593621 NaN 0 2.17 -3.81<br />
272 RB10458_Rnpc2-prov protein 5671130 5670510 NaN 0 1.54 -2.31<br />
370 RB10627_Chaperonin Cpn10 5769148 5769429 NaN 2.02 2.48 -3.65<br />
452 RB10755_hypothetical protein 5839576 5839451 NaN 0 NaN -1.5<br />
728 RB11223_ArsR family transcriptional regulator 6084224 6084586 NaN 0 NaN -1.53<br />
1035 RB11731_conserved hypothetical protein 6321063 6320896 NaN 0 NaN -2.17<br />
1041 RB11742_conserved hypothetical protein, secreted 6323302 6326160 NaN 0 NaN -1.57<br />
1095 RB11847_Peptide methionine sulfoxide reductase msrA 6379337 6380068 1.8.4.6 NaN 0 NaN -1.97<br />
1229 RB12066_hypothetical protein 6510147 6510554 NaN 0 2.33 -4.2<br />
1302 RB12197_Initiation factor 3 6577659 6577129 NaN 0 NaN -1.67<br />
1348 RB12266_conserved hypothetical protein 6602558 6602755 NaN 0 NaN -1.56<br />
1473 RB12454_conserved hypothetical protein 6703647 6703048 NaN 0 NaN -1.53<br />
1648 RB12744_conserved hypothetical protein 6868430 6868320 NaN 0 NaN -1.58<br />
1708 RB12831_Glycosyltransferase 6921326 6920532 NaN 0 NaN -1.78<br />
1728 RB12861_Oligopeptide transport system permease protein OppB. 6932929 6932000 NaN 0 NaN -1.72<br />
1895 RB1316_membrane protein 664924 665478 NaN 0 NaN -1.56<br />
1910 RB13186_subtilisin proteinase-like protein 7081960 7079642 NaN 0 NaN -1.55<br />
1927 RB13214_conserved hypothetical protein 7090176 7090286 NaN 0 NaN -1.7<br />
2070 RB1461_membrane protein 743517 742963 NaN 0 NaN -1.68<br />
2166 RB1618_hypothetical protein 833743 834036 NaN 0 NaN -1.58<br />
2367 RB2_secreted protein 182 3 NaN 0 NaN -1.55<br />
2782 RB264_Ribosomal protein S21 132677 132871 NaN 0 NaN -1.78<br />
2807 RB2682_hypothetical protein 1393781 1393530 0 0 NaN -3.2<br />
2938 RB2900_hypothetical protein 1506927 1507199 NaN 2.08 1.94 -3.22<br />
3281 RB344_conserved hypothetical protein, secreted 173795 171693 NaN 0 NaN -1.67<br />
4072 RB4642_conserved hypothetical protein 2377301 2377558 NaN 0 NaN -2.74<br />
4448 RB520_conserved hypothetical protein 284989 286116 NaN 0 NaN -2.44<br />
4679 RB5567_hypothetical protein 2897766 2897566 NaN 0 NaN -2.81<br />
6055 RB7818_Ribosomal protein S12/S23 4185730 4186095 NaN 0 NaN -2.42<br />
7305 RB9959_hypothetical protein 5366417 5366220 NaN 0 NaN -2.49<br />
1854 RB13095_conserved hypothetical protein 7026753 7027442 NaN 0 NaN -2.85
Cluster 21<br />
Original row UID Start Stop EC 62 h vs 44 h 82 h va 62 h 96 h vs 82 h 240 h vs 82 h<br />
7325 RB9999_conserved hypothetical protein-putative transcriptional regulator 5386828 5385983 NaN 2.22 NaN -2.42<br />
174 RB10279_heat shock protein, Hsp20 family 5557118 5557573 NaN 2.63 NaN -3.63<br />
175 RB10283_small heat shock protein 5557737 5558060 NaN 2.58 NaN -3.8<br />
431 RB10728_secreted protein 5831649 5832047 NaN 1.7 NaN -1.91<br />
534 RB10896_secreted protein 5917040 5916555 NaN 1.98 NaN -2.83<br />
656 RB11107_secreted protein 6021425 6022102 NaN 1.53 NaN -3.37<br />
740 RB11242_RNA polymerase sigma-70 factor, ECF subfamily 6095778 6096383 NaN 2.49 NaN -1.97<br />
1353 RB12276_conserved hypothetical protein 6605112 6605444 NaN 1.53 NaN -3.09<br />
1387 RB12323_conserved hypothetical protein 6627001 6627747 NaN 1.77 NaN -1.85<br />
1852 RB13093_secreted protein 7025577 7025101 NaN 2.29 NaN -2.42<br />
2219 RB1722_hypothetical protein 897688 899643 NaN 2.37 NaN NaN<br />
3404 RB3630_conserved hypothetical protein 1888468 1889409 NaN 2.06 NaN NaN<br />
3673 RB402_hypothetical protein 212820 213275 NaN 1.57 NaN -2.2<br />
3935 RB4438_Pyridoxamine 5'-phosphate oxidase- 2278247 2278729 NaN 1.74 NaN -3.01<br />
4096 RB4676_hypothetical protein 2391079 2390414 NaN 1.75 NaN -1.86<br />
4190 RB4809_membrane protein 2464977 2465273 NaN 2.08 NaN -2.34<br />
4227 RB4867_SmpB RNA-binding protein 2496354 2495722 NaN 1.8 NaN -2.17<br />
4849 RB5848_dnaK suppressor protein, 3066328 3065906 NaN 1.99 NaN -4.21<br />
5304 RB6595_Rickettsia 17 kDa surface antigen 3506173 3505469 NaN 1.9 NaN -1.55<br />
5571 RB7039_hypothetical protein 3743573 3742965 NaN 1.8 NaN -1.69<br />
5923 RB7612_hypothetical protein 4092907 4093317 NaN 1.55 NaN -1.82<br />
6798 RB9105_Heat shock protein Hsp70 4868834 4866720 NaN 2.31 NaN -3.7<br />
1269 RB12137_ATP-dependent Clp protease adaptor protein ClpS 6550288 6550638 NaN 1.47 NaN -1.79<br />
1628 RB12702_secreted protein 6844877 6844083 NaN 1.53 NaN -1.46
Cluster 22<br />
Original row UID Start Stop EC 62 h vs 44 h 82 h va 62 h 96 h vs 82 h 240 h vs 82 h<br />
3743 RB4147_hypothetical protein 2131928 2132338 NaN 1.54 NaN 0<br />
3992 RB4517_Transcriptional coactivator/pterin dehydratase 2315930 2316283 NaN 1.75 NaN 0<br />
4194 RB4812_secreted protein 2467011 2467289 NaN 1.88 NaN 0<br />
4896 RB5927_protein containing DUF1501 3107242 3105905 NaN 1.5 0 0<br />
5020 RB6144_hypothetical protein 3228305 3228189 NaN 1.46 NaN 0<br />
5763 RB7362_hypothetical protein 3939758 3939501 NaN 1.77 0 0<br />
6410 RB8421_conserved hypothetical protein, membrane 4501840 4502310 NaN 1.55 NaN 0<br />
6957 RB9372_Imidazole glycerol phosphate synthase subunit hisH 5021363 5020746 2.4.2.- NaN 2.43 -1.48 0<br />
7116 RB9635_hypothetical protein 5187031 5185973 NaN 1.51 NaN 0<br />
7319 RB9986_hypothetical protein 5380717 5380379 NaN 1.49 NaN 0<br />
183 RB10299_ATP-dependent carboligase related to biotin carboxylase (ORFY) 5563036 5564403 -1.7 2.45 -1.73 0<br />
369 RB10623_secreted protein 5769140 5768958 -2.4 2.03 0 -1.85<br />
371 RB10629_GroEL 5769500 5771119 NaN 1.76 NaN 0<br />
854 RB11421_60-kDa SS-A/Ro ribonucleoprotein 6195538 6193880 NaN 1.73 NaN 0<br />
960 RB11618_type I restriction-modification system, M subunit, 6263708 6265366 NaN 1.58 NaN 0<br />
1085 RB1183_membrane protein 605059 605553 NaN 1.54 0 0<br />
1136 RB11919_Dihydroxy-acid and 6-phosphogluconate dehydratase 6417713 6415698 NaN 1.67 NaN 0<br />
1354 RB12278_conserved hypothetical protein 6605390 6605548 NaN 3.27 0 0<br />
1434 RB12395_conserved hypothetical protein 6670730 6670572 NaN 1.66 0 0<br />
1851 RB13092_conserved hypothetical protein 7025003 7025143 NaN 1.64 NaN 0<br />
1916 RB13194_conserved hypothetical protein 7084723 7084920 NaN 1.94 0 0<br />
1920 RB13202_conserved hypothetical protein 7086189 7086052 NaN 1.68 NaN 0<br />
2062 RB1449_hypothetical protein 736500 736697 NaN 2.71 NaN 0<br />
2655 RB2459_hypothetical protein 1280452 1280204 NaN 1.58 NaN 0<br />
3275 RB3430_conserved hypothetical protein, membrane 1772646 1773392 NaN 1.54 NaN 0<br />
3493 RB3751_NAD-dependent epimerase/dehydratase 1938953 1940068 NaN 1.95 NaN 0<br />
3852 RB4308_protein containing DUF1586 2221461 2221222 NaN 2.04 NaN 0<br />
4473 RB5238_hypothetical protein 2697775 2697996 NaN 1.79 NaN NaN<br />
4748 RB568_hypothetical protein 312345 312112 0 3.58 0 -1.54<br />
5461 RB6848_secreted protein 3642216 3642443 NaN 1.86 NaN NaN<br />
5924 RB7613_hypothetical protein 4093558 4093418 NaN 1.71 0 1.5<br />
1230 RB12067_conserved hypothetical protein 6510236 6510559 NaN 1.86 NaN NaN<br />
1277 RB12150_conserved hypothetical protein 6556901 6557062 NaN 1.75 0 -1.54<br />
1890 RB13150_sialic acid-specific 9-O-acetylesterase 7059586 7058003 3.1.1.53 NaN 1.83 NaN NaN<br />
3132 RB3217_S-adenosylmethionine decarboxylase proenzyme 1654745 1654251 4.1.1.50 0 1.82 NaN 0<br />
5400 RB6751_chaperone ClpB 3586111 3588690 NaN 1.78 NaN NaN<br />
4253 RB4903_xylulose-5-phosphate/fructose-6-phosphate phosphoketolase 2520925 2523306 4.1.2.9 NaN 1.91 NaN NaN
Cluster 23<br />
Original row UID Start Stop EC 62 h vs 44 h 62 h vs 82 h 96 h vs 82 h 240 h vs 82 h<br />
7290 RB9930_hypothetical protein 5349331 5348975 NaN 0 NaN -4.2<br />
50 RB10077_secreted protein 5436817 5437083 NaN 0 NaN -4.91<br />
554 RB10931_hypothetical protein 5933742 5933906 NaN 0 NaN -3.45<br />
556 RB10934_CsbD-like 5934135 5934341 NaN 0 NaN -4.35<br />
564 RB10948_conserved hypothetical protein, secreted 5938575 5939036 NaN 0 NaN -4.57<br />
569 RB10956_hypothetical protein 5940336 5940689 NaN 0 NaN -3.21<br />
697 RB11176_protein containing DUF442 6065605 6065144 NaN 0 NaN -3.19<br />
1218 RB12046_conserved hypothetical protein 6497841 6497996 NaN 0 NaN -3.47<br />
2031 RB1394_hypothetical protein 711994 711758 NaN 0 NaN -3.17<br />
3872 RB4347_conserved hypothetical protein 2235735 2236205 NaN 0 NaN -3.32<br />
3930 RB4429_conserved hypothetical protein 2275996 2276265 NaN 0 NaN -3.08<br />
3974 RB4493_hypothetical protein 2302374 2302123 NaN 0 NaN -4.83<br />
3980 RB4500_conserved hypothetical protein, secreted 2303645 2303908 NaN 0 NaN -4.11<br />
5179 RB6403_hypothetical protein 3369939 3370853 NaN 0 NaN -3.37<br />
1405 RB12348_FxsA cytoplasmic membrane protein 6645520 6644738 NaN -2.33 NaN -5.77<br />
751 RB11260_dnaK suppressor protein, 6107202 6106837 NaN -1.66 NaN -4.07
Cluster 24<br />
Original row UID Start Stop EC 62 h vs 44 h 82 h va 62 h 96 h vs 82 h 240 h vs 82 h<br />
1359 RB12285_phospholipid N-methyltransferase 6609391 6610065 2.1.1.17 NaN 0 2.19 0<br />
3486 RB3742_Flagellin, N-terminal 1935693 1937849 NaN 0 2.18 0<br />
3797 RB4230_conserved hypothetical protein 2168783 2167788 NaN 0 2.31 0<br />
3182 RB3295_secreted protein 1692681 1692259 NaN 0 2.35 0<br />
3290 RB3455_hypothetical protein 1785458 1785631 NaN 0 2.38 0<br />
575 RB10967_quinone oxidoreductase-like protein 5944040 5943015 NaN 0 2.4 0<br />
750 RB11258_conserved hypothetical protein, membrane 6106840 6106127 NaN 0 2.39 0<br />
861 RB11434_conserved hypothetical protein 6197442 6197233 NaN 0 2.5 0<br />
2939 RB2901_conserved hypothetical protein, secreted 1507210 1508388 NaN 0 2.39 0<br />
3694 RB4060_TadE-like 2095403 2094831 NaN 0 2.42 0<br />
3865 RB4336_conserved hypothetical protein 2231697 2232815 NaN 0 2.54 0<br />
3895 RB4381_hypothetical protein 2255621 2255442 NaN 0 2.5 0<br />
1921 RB13205_ hypothetical protein 7086564 7086337 NaN 0 2.6 0<br />
3921 RB4419_MgtC/SapB transporter 2272912 2273448 NaN 0 2.71 0<br />
6633 RB8814_hypothetical protein 4687433 4687669 NaN 0 2.75 0
Cluster 25<br />
Original row UID Start Stop EC 62 h vs 44 h 82 h va 62 h 96 h vs 82 h 240 h vs 82 h<br />
6721 RB8959_hypothetical protein 4777121 4775133 0 1.54 1.77 0<br />
513 RB10867_hypothetical protein 5899359 5899982 0 0 1.71 0<br />
559 RB10939_conserved hypothetical protein 5934628 5934816 NaN 0 1.69 0<br />
609 RB11021_ATP-binding protein of ABC transporter 5975385 5974498 NaN 0 1.6 0<br />
696 RB11174_metallo-beta-lactamase family protein 6065046 6063637 NaN 0 1.63 0<br />
889 RB11488_oxidoreductase, short-chain dehydrogenase/reductase family 6213893 6214690 0 1.61 1.74 0<br />
893 RB11492_conserved hypothetical protein, membrane 6216103 6216390 NaN 0 1.64 0<br />
902 RB11505_conserved hypothetical protein, secreted 6221829 6221230 0 0 1.68 0<br />
1711 RB12837_conserved hypothetical protein 6921732 6921929 0 0 1.92 0<br />
2516 RB2244_Glutathione peroxidase 1178675 1179253 1.11.1.12 0 0 1.85 0<br />
3061 RB309_Magnesium chelatase, ChlI subunit 156441 154993 6.6.1.1 0 0 1.94 0<br />
3183 RB3298_hypothetical protein 1692797 1692678 0 0 2.24 0<br />
3689 RB4051_hypothetical protein 2092752 2092210 NaN 0 1.68 0<br />
3896 RB4382_Diaminopimelate decarboxylase 2257285 2255618 4.1.1.20 -1.47 0 1.53 0<br />
3909 RB4399_zinc-binding oxidoreductase 2265237 2264251 0 0 2.02 0<br />
3925 RB4423_cytidine and deoxycytidylate deaminase family protein 2275085 2274588 NaN 0 1.72 0<br />
3934 RB4436_membrane protein 2277820 2278092 0 0 1.84 0<br />
3951 RB4460_conserved hypothetical protein, membrane 2289696 2288428 NaN 0 1.51 0<br />
3964 RB4478_membrane protein 2296122 2296640 0 0 2.3 0<br />
3970 RB4487_two-component system response regulator 2299859 2301403 0 0 1.93 0<br />
4428 RB5173_hypothetical protein 2660933 2660799 0 0 2.75 0<br />
4490 RB5266_hypothetical protein 2715721 2715584 0 0 1.6 0<br />
4629 RB548_1,4-alpha-glucan branching enzyme 306230 304329 2.4.1.18 NaN 0 1.51 0<br />
5659 RB7178_hypothetical protein 3806924 3807061 NaN 0 1.7 0<br />
6888 RB9261_conserved hypothetical protein 4952589 4953827 NaN 0 1.63 0<br />
6965 RB9386_secreted protein 5034937 5033729 NaN 0 1.59 NaN<br />
7007 RB9460_hypothetical protein 5078860 5078621 0 0 1.93 0<br />
1922 RB13207_secreted protein 7086761 7086585 0 0 3.98 0<br />
2517 RB2247_conserved hypothetical protein containing YTV domains 1181238 1179499 0 0 3.12 0<br />
620 RB11041_secreted protein containing DUF1559 5987234 5988283 NaN 0 1.79 0<br />
753 RB11266_3',5'-cyclic-nucleotide phosphodiesterase 6108475 6109389 NaN 0 1.84 0<br />
1555 RB1259_hypothetical protein 642452 642631 NaN 0 1.79 0<br />
1707 RB12830_conserved hypothetical protein 6920508 6920200 NaN 0 1.83 0<br />
2255 RB1787_hypothetical protein 925904 925686 NaN 0 1.88 0<br />
3913 RB4404_oxidoreductase, short chain dehydrogenase/reductase family 2265539 2266447 NaN 0 1.82 0<br />
5097 RB6276_Histone-like bacterial DNA-binding protein 3297504 3297187 NaN 0 1.82 NaN<br />
7241 RB9852_RNA polymerase ECF-type sigma factor 5304982 5305653 NaN 0 1.94 0<br />
7268 RB9897_hypothetical protein 5333773 5333549 NaN 0 1.82 0<br />
196 RB10326_sulfate permease family protein 5580833 5582599 NaN 0 1.84 0<br />
290 RB10483_conserved hypothetical protein 5685437 5685838 NaN 0 1.84 0<br />
681 RB11150_NADH:flavin oxidoreductase/NADH oxidase 6046862 6048019 NaN 0 1.94 0<br />
1412 RB12362_bacterioferritin comigratory protein 6653786 6652962 NaN 0 2.03 0<br />
2616 RB2386_RND efflux membrane fusion protein 1244320 1242734 NaN 0 1.98 0<br />
6168 RB8011_secreted protein containing DUF1571 4282298 4281114 NaN 0 2.06 0<br />
6951 RB9360_Protein of unknown function, UPF0027 5015563 5014031 NaN 0 2.01 0<br />
132 RB10209_H+-transporting two-sector ATPase, C subunit 5515135 5515467 3.6.3.14 NaN 0 2.01 0<br />
574 RB10966_Protein of unknown function, UPF0057 5942934 5942752 NaN 0 2.02 0<br />
3446 RB3686_oxidoreductase, zinc-binding 1910523 1909540 NaN 0 2.12 0<br />
3968 RB4484_Peptidase M20 2297267 2298565 3.-.-.- NaN 0 2.16 0