Data sheet Sulfo-6-Methyl-Tetrazine-DBCO - Jena Bioscience

Data sheet
Sulfo-6-Methyl-Tetrazine-DBCO
Sulfo-6-Methyl-Tetrazine-Dibenzylcyclooctyne
Triethylammonium salt
Cat. No.
CLK-1022-10
CLK-1022-25
CLK-1022-100
Amount
10 mg
25 mg
100 mg
Applications
Protein-protein conjugates
Protein-peptide conjugates
Protein-small molecule conjugates
O
O
18F radiolabelling
H 3 C
N
N
N
N
N
H
O
S
O –
O
N
H
O
N
Protein-oligonucleotide conjugates
Surface modification
Molecular Formula: C 32 H 28 N 7 O 6 S (Anion)
Molecular Weight: 638.67 g/mol (Anion)
Purity: > 95 % (HPLC)
Appearance: solid
Solubility: DMF, DMSO, MeOH, water
Storage conditions: store at -20 °C
Shelf life: 12 months
For research use only!
Description
Sulfo-6-Methyl-Tetrazine-DBCO is a water-soluble reagent
for converting of azido-modified peptides or biopolymers
into tetrazine-modified peptides or biopolymers. When
applied in stoichiometric amount Sulfo-6-Methyl-Tetrazine-
DBCO can serve as a heterobifunctional azide-to-TCO
crosslinker for covalently linking azido- and TCO-modified
peptides or biopolymers. Once a peptide or biopolymer
is tetrazine-labeled, it can be reacted with a TCO-labeled
molecule to produce a stable conjugate.
Important Product Information
• Molecules to be reacted with Sulfo-6-Methyl-
Tetrazine-DBCO must have available azides.
• Do not use buffers that contain azides.
Procedure for Azide-to-Tetrazine Conversion
• Prepare the azido-containing sample in reaction
buffer or organic solvent.
• Dissolve Sulfo-6-Methyl-Tetrazine-DBCO in organic
solvent.
• Add 2-10 fold excess of Sulfo-6-Methyl-Tetrazine-
DBCO to azido-containing sample.
• Incubate the reaction at room temperature for 1-4
hour. Incubation at 4°C requires 2-12 hours.
Jena Bioscience GmbH | Löbstedter Str. 80 | 07749 Jena, Germany | Tel.:+49-3641-6285 000 | Fax:+49-3641-6285 100
http://www.jenabioscience.com
Last update: Jun 14, 2013

Data sheet
Sulfo-6-Methyl-Tetrazine-DBCO
Sulfo-6-Methyl-Tetrazine-Dibenzylcyclooctyne
Triethylammonium salt
• Remove excess reagent by desalting the labeled
protein through a desalt spin column or by azidemodified
agarose.
Procedure for TCO-Tetrazine Conjugation
• Calculate 1.1 - 5 fold molar excess of tetrazineor
TCO-modified biopolymer over the corresponding
complimentary reagent.
• Mix calculated amount of tetrazine-labeled biopolymer
with desired amount of TCO-modified biopolymer.
• Allow reaction to proceed for 30 minutes at room
temperature. Longer reaction time might improve
conjugation efficiency.
Selected References:
Karver et al. (2012) Bioorthogonal Reaction Pairs Enable Simultaneous,
Selective, Multi-Target Imaging. Angew. Chem. Int. Ed. 51:920.
Devaraj et al. (2009) Fast and Sensitive Pre-Targeted Labeling of
Cancer Cells through a Tetrazine/trans-Cyclooctene Cycloaddition.
Angew. Chem. Int. Ed. 48:7013.
Haun et al. (2009) Probing Intracellular Biomarkers and Mediators of
Cell Activation Using Nanosensor and Bioorthogonal Chemistry. ACS
Nano. 5:3204.
Blackman et al. (2008) Tetrazine Ligation: Fast Bioconjugation Based
on Inverse-Electron-Demand Diels-Alder Reactivity. J. Am. Chem. Soc.
130:13518.
Devaraj et al. (2008) Tetrazine-Based Cycloadditions: Application to
Pretargeted Live Cell Imaging. Bioconjugate Chem. 19:2297.
• Store conjugate at 4°C until ready for purification or
use.
Jena Bioscience GmbH | Löbstedter Str. 80 | 07749 Jena, Germany | Tel.:+49-3641-6285 000 | Fax:+49-3641-6285 100
http://www.jenabioscience.com
Last update: Jun 14, 2013