Data sheet - Jena Bioscience

Data sheet
5-Methyl-dCTP
5-Methyl-2’-deoxy-cytidine-5’-triphosphate, Sodium salt
Cat. No.
NU-1125S
NU-1125L
Amount
100 Units
500 Units
Applications:
Analyzing methylation state of DNA [0pt][1]
Cloning of identical sequences, FISH [0pt][2]
NH 2
HO
O O O
P P P
O O O
OH OH OH
H 3 C
O
N
N
O
X-ray of NTP-pyrophosphate hydrolase [0pt][3]
Substrate specificity of Nudix hydrolase (Orf 135) [0pt][4]
Incorporation by PCR [0pt][5]
OH
C 10 H 18 N 3 O 13 P 3
481. 18
Molecular Formula: C 10 H 18 N 3 O 13 P 3 (free acid)
481. 005253
C 25.0% H 3.8% N 8.7% O 43.2% P 19.3%
Molecular Weight: 481.18 (free acid)
Purity: > 95%, clear aqueous solution, pH 7.5
Spectroscopic properties: λ max 277 nm;
ɛ 9000
Storage conditions:
Short term exposure (up to 1 week cumulative) to ambient
temperature possible. Long term storage at < -20°C.
For research use only!
* 1 unit = 1 µl of a 10 mM solution
Selected References:
[1] Kaito et al. (2001) Activation of the maternally preset program of
apoptosis by microinjection of 5-aza-2’-deoxycytidine and
5-methyl-2’-deoxycytidine-5’-triphosphate in Xenopus laevis embryos.
Dev Growth Differ. 43(4):383.
[2] Zabarovska et al.(2000) CIS-cloning of identical sequences
between two complex genoms. Chromosome Research 8:77.
[3] Wu et al. (2007) Crystal structure of RS21-C6, involved in
nucleoside triphosphate pyrophosphohydrolysis. J. Mol. Biol.
367:1405.
[4] Kamiya et al. (2004) Important amino acids in the
phosphohydrolase module of Escherichia coli Orf135. Biochem
Biophys Res Commun. 323(3):1063.
[5] Dezhurov et al. (2005) A new highly efficient photoreactive
analogue of dCTP. Synthesis, characterization, and application in
photoaffinity modification of DNA binding proteins. Bioconjug. Chem.
16(1):215.
Szwagierczak A et al. (2011) Characterization of PvuRts1I
endonuclease as a tool to investigate genomic
5-hydroxymethylcytosine. Nucleic Acids Res. 39 (12):5149.
Zeschnigk et al. (2009) Massive parallel bisulfite sequencing of CG-rich
DNA fragments reveals that methylation of many X-chromosomal CpG
islands in female blood DNA is incomplete. Human Molecular Genetics
18 (8):1439.
Frauer et al. (2009) A versatile non-radioactive assay for DNA
methyltransferase activity and DNA binding. Nucleic Acids Research
37 (3):e22.
Holliday et al. (2002) DNA methylation and epigenetic inheritance.
Methods. 27(2):179.
Wong et al. (1997) A novel method for producing partial restriction
digestion of DNA fragments by PCR with 5-methyl-CTP. Nucleic Acids
Res. 25(20):4169.
Jena Bioscience GmbH | Löbstedter Str. 80 | 07749 Jena, Germany | Tel.:+49-3641-6285 000 | Fax:+49-3641-6285 100
http://www.jenabioscience.com
Last update: Jul 10, 2013

Data sheet
5-Methyl-dCTP
5-Methyl-2’-deoxy-cytidine-5’-triphosphate, Sodium salt
Chen et al. (1993) Direct induction of DNA hypermethylation in sea
urchin embryos by microinjection of 5-methyl dCTP stimulates early
histone gene expression and leads to developmental arrest. Dev Biol.
155(1):75.
Ito, A. et al. (1998) Blood 91-9:3210
Lefaucheur, L. et al. (1998) J. Anim. Sci. 76:1584
Nelson, P. S. et al. (1993) Nucl. Acid. Res. 21-3:681
Asamizu, E. et al. (1999) DNA Research 6:369
Wong, K. K. et al. (1991) Nucl. Acid. Res. 19-5:1081
Jena Bioscience GmbH | Löbstedter Str. 80 | 07749 Jena, Germany | Tel.:+49-3641-6285 000 | Fax:+49-3641-6285 100
http://www.jenabioscience.com
Last update: Jul 10, 2013