Data sheet - Jena Bioscience
Data sheet - Jena Bioscience
Data sheet - Jena Bioscience
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<strong>Data</strong> <strong>sheet</strong><br />
5-Methyl-dCTP<br />
5-Methyl-2’-deoxy-cytidine-5’-triphosphate, Sodium salt<br />
Cat. No.<br />
NU-1125S<br />
NU-1125L<br />
Amount<br />
100 Units<br />
500 Units<br />
Applications:<br />
Analyzing methylation state of DNA [0pt][1]<br />
Cloning of identical sequences, FISH [0pt][2]<br />
NH 2<br />
HO<br />
O O O<br />
P P P<br />
O O O<br />
OH OH OH<br />
H 3 C<br />
O<br />
N<br />
N<br />
O<br />
X-ray of NTP-pyrophosphate hydrolase [0pt][3]<br />
Substrate specificity of Nudix hydrolase (Orf 135) [0pt][4]<br />
Incorporation by PCR [0pt][5]<br />
OH<br />
C 10 H 18 N 3 O 13 P 3<br />
481. 18<br />
Molecular Formula: C 10 H 18 N 3 O 13 P 3 (free acid)<br />
481. 005253<br />
C 25.0% H 3.8% N 8.7% O 43.2% P 19.3%<br />
Molecular Weight: 481.18 (free acid)<br />
Purity: > 95%, clear aqueous solution, pH 7.5<br />
Spectroscopic properties: λ max 277 nm;<br />
ɛ 9000<br />
Storage conditions:<br />
Short term exposure (up to 1 week cumulative) to ambient<br />
temperature possible. Long term storage at < -20°C.<br />
For research use only!<br />
* 1 unit = 1 µl of a 10 mM solution<br />
Selected References:<br />
[1] Kaito et al. (2001) Activation of the maternally preset program of<br />
apoptosis by microinjection of 5-aza-2’-deoxycytidine and<br />
5-methyl-2’-deoxycytidine-5’-triphosphate in Xenopus laevis embryos.<br />
Dev Growth Differ. 43(4):383.<br />
[2] Zabarovska et al.(2000) CIS-cloning of identical sequences<br />
between two complex genoms. Chromosome Research 8:77.<br />
[3] Wu et al. (2007) Crystal structure of RS21-C6, involved in<br />
nucleoside triphosphate pyrophosphohydrolysis. J. Mol. Biol.<br />
367:1405.<br />
[4] Kamiya et al. (2004) Important amino acids in the<br />
phosphohydrolase module of Escherichia coli Orf135. Biochem<br />
Biophys Res Commun. 323(3):1063.<br />
[5] Dezhurov et al. (2005) A new highly efficient photoreactive<br />
analogue of dCTP. Synthesis, characterization, and application in<br />
photoaffinity modification of DNA binding proteins. Bioconjug. Chem.<br />
16(1):215.<br />
Szwagierczak A et al. (2011) Characterization of PvuRts1I<br />
endonuclease as a tool to investigate genomic<br />
5-hydroxymethylcytosine. Nucleic Acids Res. 39 (12):5149.<br />
Zeschnigk et al. (2009) Massive parallel bisulfite sequencing of CG-rich<br />
DNA fragments reveals that methylation of many X-chromosomal CpG<br />
islands in female blood DNA is incomplete. Human Molecular Genetics<br />
18 (8):1439.<br />
Frauer et al. (2009) A versatile non-radioactive assay for DNA<br />
methyltransferase activity and DNA binding. Nucleic Acids Research<br />
37 (3):e22.<br />
Holliday et al. (2002) DNA methylation and epigenetic inheritance.<br />
Methods. 27(2):179.<br />
Wong et al. (1997) A novel method for producing partial restriction<br />
digestion of DNA fragments by PCR with 5-methyl-CTP. Nucleic Acids<br />
Res. 25(20):4169.<br />
<strong>Jena</strong> <strong>Bioscience</strong> GmbH | Löbstedter Str. 80 | 07749 <strong>Jena</strong>, Germany | Tel.:+49-3641-6285 000 | Fax:+49-3641-6285 100<br />
http://www.jenabioscience.com<br />
Last update: Jul 10, 2013
<strong>Data</strong> <strong>sheet</strong><br />
5-Methyl-dCTP<br />
5-Methyl-2’-deoxy-cytidine-5’-triphosphate, Sodium salt<br />
Chen et al. (1993) Direct induction of DNA hypermethylation in sea<br />
urchin embryos by microinjection of 5-methyl dCTP stimulates early<br />
histone gene expression and leads to developmental arrest. Dev Biol.<br />
155(1):75.<br />
Ito, A. et al. (1998) Blood 91-9:3210<br />
Lefaucheur, L. et al. (1998) J. Anim. Sci. 76:1584<br />
Nelson, P. S. et al. (1993) Nucl. Acid. Res. 21-3:681<br />
Asamizu, E. et al. (1999) DNA Research 6:369<br />
Wong, K. K. et al. (1991) Nucl. Acid. Res. 19-5:1081<br />
<strong>Jena</strong> <strong>Bioscience</strong> GmbH | Löbstedter Str. 80 | 07749 <strong>Jena</strong>, Germany | Tel.:+49-3641-6285 000 | Fax:+49-3641-6285 100<br />
http://www.jenabioscience.com<br />
Last update: Jul 10, 2013