presence of aflatoxins in smoked-dried fish sold in abeokuta, ogun ...

. 250ml graduated cylinder
c. Container with 125ml capacity.
d. Neogen filter syringes. Whatman #1 filter paper
e. Sample collection tubes.
8. High-speed blender.
9. Agri-Grind grinder or blender.
10. Scale capable of weighing 5-50grams
11. Microwell reader with a 650nm filter.
12. Pipettor, 12-channel.
13. Pipettor, 100µl.
14. Pipette tips for 100µl and 12-channel pipettors.
15. Towel as an absorbent material.
16. Plastic bucket for use as a waste receptacle.
17. Microwell holder.
18. Timer.
19. Waterproof marker.
20. Wash bottle.
21. 2 reagents boats for 12-channel pipettor.
22. Good quality grade distilled water.
3.4 Sample Preparation and Extraction
1. The collected samples (smoked-dried fish) were ground into powdery form with the use
of high-speed blender, thoroughly mixed together and made into composite, followed by
weighing on an electronic scale. 5 grams of the representative sample was put into an
extraction cup.
2. 25ml of 70% methanol was added, the extraction cup was covered and shaked vigorously
using hand for 3 minutes, then the mixture was allowed to settle down.
3. The extract was filtered by pouring 5ml through a Whatman #1 filter syringe, and filtrate
was collected as a sample.
4. The sample is now ready for testing.
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