Shakers

Selecting the Right Model Fermentor/Bioreactor
Model
FibraStage
BioFlo 110
Ferm/Bioreactor
BioFlo 310
Fermentor
CelliGen 310
Bioreactor
BioFlo 415
Fermentor
BioFlo 510
Fermentor
CelliGen 510
Bioreactor
BioFlo Pro
Fermentor
CelliGen Pro
Bioreactor
(W) 500 mL
(T) Total Volume
(T) 1.3 - 14 L
(W) 0.4 - 10.5 L
(T) 2.5 - 14 L
(W) 0.75 - 10.5 L
(T) 2.5 - 14 L
(W) 0.75 - 10.5 L
(T) 7 - 19.5 L
(W) 2.0 - 15.5 L
(T) 19.5 - 40 L
(W) 5.2 - 32 L
(T) 19.5 - 40 L
(W) 5.2 - 32 L
(T) 75 - 3000 L
(W) 32 - 2400 L
(T) 75 - 650 L
(W) 18.75 - 520 L
(W) Working Volume
D
A
A
A
SIP
SIP
SIP
SIP
SIP
(A) Autoclavable
(SIP) Sterilizable-In-Place
(D) Disposable
•
•
•
•
•
Plant/Algae
Bacterial/Yeast/Fungi
•
•
•
•
•
•
•
•
•
Insect
Mammalian/Animal
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
Interchangeable
Vessels
•
Microprocessor
Controller
Touchscreen
Controller
Heat Induction
R
• • or
TMFC
R
•
or
TMFC
•
•
•
•
TMFC
R
or
TMFC
•
•*
•*
R or
TMFC
R
or
TMFC
R
or
•
TMFC
R
or
TMFC
Air Flow Control ◊
2/4
Gas
2/3/4
Gas
2/3/4
Gas
2/3/4
Gas
2/3/4
Gas
2/3/4
Gas
2
Gas
4
Gas
Validatable
Gas Mixing Options
Page
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44-49
• 50-51
• 54-56
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•
•
58
59
59
• 60-61
• 60-62
(◊) Air Flow Controllers: R=Rotameter, TMFC=Thermal Mass Flow Controller (*) = Programmable Automation Controller (PAC)
A Lesson in Gas Supplementation:
What’s the difference between a Rotameter and a Flow Controller?
Gas Supplementation
Depending upon the culture that is being grown, additional
gases beyond air may be required to help sustain growth.
Bacterial/yeast cultures, (Pichia, E. coli, streptomyces) often
require oxygen to supplement the flow of air entering the
system to meet the increased oxygen demands of these
cultures. Insect cells usually require air, O 2 , and CO 2 to sustain
the dissolved oxygen and pH in the vessel at levels conducive
to growth. Mammalian cells, which tend to be the most
sensitive to changes in their growth environment, typically
require Air, O 2 , N 2 , and CO 2 to attain even tighter control of
DO and pH in the medium.
Rotameters
This manually-operated device can be used to control the
flow of gas or gasses entering the system. A Rotameter uses
a graduated tube and a float to provide a visual indication of
flow rate.
Thermal Mass Flow Controllers (TMFC)
This sophisticated device is used to enter the gas flow rate on
the operator interface/display screen, and automatically control
the actual gas flow rate entering your fermentor/
bioreactor vessel. Setpoint and process (current) value are
displayed in SLPM (Standard Liters Per Minute).
1.732.287.1200 • 1.800.631.5417 • Fax: 1.732.287.4222 • bioinfo@nbsc.com
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