Control Cuvette

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Method Vanzetti, G.,J. Lab & Clin Med 67:1, 116 (1966). Principle Sodium deoxycholate hemolyses the erythrocytes and hemoglobin is released. Sodium nitrite converts hemoglobin to methemoglobin which, together with sodium azide, gives azidemethemoglobin. The absorbance is measured at two wavelengths (570 and 880 mm) in order to compensate for turbidity in the sample. 12 Storage and environmental requirements The microcuvette The photometer Calibration Sample Material The microcuvettes are to be stored at room temperature (15-30 °C, 59-86 °F). The expiry date is printed on each container. Once opened the microcuvettes are stable for three months. Always keep the container properly closed. The photometer can be stored at temperature 0-50 °C (32-122 °F). The operating temperature is 15-40 °C (59-104 °F). Allow the photometer to reach ambient temperature before use. The photometer should not be operated at high (>90 % noncondensing) humidity. The photometer is delivered calibrated against the hemiglobincyanide (HiCN) method which is the international reference method for the determination of the total hemoglobin concentration in blood. After the factory calibration, which is carried out at ≈14 g/dl (140 g/l, 8.7 mmol/l), a maximum deviation of ± 0.3 g/dl (± 3 g/l, 0.2 mmol/l) is tolerated. Capillary, venous or arterial blood may be used. Anticoagulant in solid form e.g. EDTA or heparin/ fluoride are recommended in order to avoid dilutional effect. Venous or arterial blood must be used within 24 hours.
Quality Control Control Cuvette Check the function of the photometer on a daily basis by measuring the control cuvette. Values obtained should not deviate from the assigned value on the control cuvette card more than ±0.3 g/dl (±3 g/l, 0.2 mmol/l). Hemoglobin Controls, whole blood or hemolysate A control of the total system i.e. photometer and microcuvette can be obtained by using whole blood or hemolysate with a given hemoglobin value. The blood control used should have a viscosity equal to that of normal blood. It is important that the control blood is mixed well before use, preferably ten minutes in a blood mixer. HemoCue AB supplies controls in different concentrations. N.B.: Many whole blood or hemolysate controls that are available commercially contain additives that cause turbidity (cloudiness). As the photometer compensates for turbidity it can give a lower hemoglobin value than that given for the control blood/ hemolysate. Specifications For performance characteristics, see HemoCue B- Hemoglobin microcuvette instructions for use. Measuring Range 0-25.6 g/ldl (0-256 g/l, 0-15.9 mmol/l) Values above 23.5 g/dl (235 g/l, 14.6 mmol/l) should be confirmed using a suitable laboratory method. Values above 25.6 g/dl (256 g/l, 15.9 mmol/l) will display an error code: “ERROR 999 ” or ERROR HHH”. Reference Values Adult Males 13.0–18.0 g/dl (130-180 g/l, 3.1-11.2 mmol/l) Adult Females 11.0–16.0 g/dl (110-160 g/l, 6.8-9.9 mmol/l) Infants, after neonatal period 10.0–14.0 g/dl (100-140 g/l, 6.2-8.7 mmol/l) Children, two years to teenage: gradual increase to adult normals. Due to the wide range of conditions (dietary geographical, etc) which affect normal values, it is recommended that each laboratory establish its own normal range. Limitations The HemoCue B-Hemoglobin photometer is only to be used together with HemoCue B-Hemoglobin microcuvettes. The HemoCue B-Hemoglobin microcuvettes are for in vitro diagnostic use only. For further limitations of the procedure, see HemoCue B- Hemoglobin Microcuvette instructions for use. 13
Page 1 and 2: Blood Hemoglobin Photometer Operati
Page 3 and 4: General The HemoCue ® B-Hemoglobin
Page 5 and 6: Battery Power Underneath the photom
Page 7 and 8: Checking the Photometer Pull out th
Page 9 and 10: The thumb has moved up to the tip o
Page 14 and 15: Trouble Shooting Guide If you are u
Page 16: Guarantee The photometer carries a

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