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Control Cuvette

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Quality <strong>Control</strong><br />

<strong>Control</strong> <strong>Cuvette</strong><br />

Check the function of the photometer on a daily<br />

basis by measuring the control cuvette. Values<br />

obtained should not deviate from the assigned value<br />

on the control cuvette card more than ±0.3 g/dl<br />

(±3 g/l, 0.2 mmol/l).<br />

Hemoglobin <strong>Control</strong>s, whole blood or hemolysate<br />

A control of the total system i.e. photometer and<br />

microcuvette can be obtained by using whole blood<br />

or hemolysate with a given hemoglobin value. The<br />

blood control used should have a viscosity equal to<br />

that of normal blood. It is important that the control<br />

blood is mixed well before use, preferably ten minutes<br />

in a blood mixer.<br />

HemoCue AB supplies controls in different concentrations.<br />

N.B.: Many whole blood or hemolysate controls that<br />

are available commercially contain additives that<br />

cause turbidity (cloudiness). As the photometer<br />

compensates for turbidity it can give a lower hemoglobin<br />

value than that given for the control blood/<br />

hemolysate.<br />

Specifications<br />

For performance characteristics, see HemoCue B-<br />

Hemoglobin microcuvette instructions for use.<br />

Measuring Range<br />

0-25.6 g/ldl (0-256 g/l, 0-15.9 mmol/l)<br />

Values above 23.5 g/dl (235 g/l, 14.6 mmol/l) should be<br />

confirmed using a suitable laboratory method.<br />

Values above 25.6 g/dl (256 g/l, 15.9 mmol/l) will display an<br />

error code: “ERROR 999 ” or ERROR HHH”.<br />

Reference Values<br />

Adult Males<br />

13.0–18.0 g/dl (130-180 g/l, 3.1-11.2 mmol/l)<br />

Adult Females<br />

11.0–16.0 g/dl (110-160 g/l, 6.8-9.9 mmol/l)<br />

Infants, after neonatal period 10.0–14.0 g/dl (100-140 g/l, 6.2-8.7 mmol/l)<br />

Children, two years to teenage: gradual increase to adult normals.<br />

Due to the wide range of conditions (dietary geographical, etc) which affect<br />

normal values, it is recommended that each laboratory establish its own<br />

normal range.<br />

Limitations<br />

The HemoCue B-Hemoglobin photometer is only to be used together<br />

with HemoCue B-Hemoglobin microcuvettes.<br />

The HemoCue B-Hemoglobin microcuvettes are for in vitro diagnostic<br />

use only. For further limitations of the procedure, see HemoCue B-<br />

Hemoglobin Microcuvette instructions for use.<br />

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