Dr. Ross Mikkelsen - Pathology - Virginia Commonwealth University

NAME
Mikkelsen, Ross B.
eRA COMMONS USER NAME
RMIKKELSEN
BIOGRAPHICAL SKETCH
Provide the following information for the key personnel and other significant contributors.
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POSITION TITLE
Professor
EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.)
INSTITUTION AND LOCATION
DEGREE
(if applicable)
YEAR(s)
FIELD OF STUDY
University of California at Santa Barbara B.A. 1964­1968 Biology
University of California at Santa Barbara Ph.D. 1968­1973 Biology
Tufts University School of Medicine, Boston MA Post­doc 1974­1978 Membrane Biochemistry
A. Positions and Honors
Positions and Employment
1973­1975 Research Fellow, Division of Radiobiology, Department of Therapeutic Radiology, Tufts
University School of Medicine, Boston, MA
1976­1978 Instructor, Division of Radiobiology, Department of Therapeutic Radiology, Tufts University
School of Medicine, Boston, MA
1978­1988 Assistant Professor, Division of Radiobiology, Department of Therapeutic Radiology, Tufts
University School of Medicine, Boston, MA
1985­1988 Assistant Professor, Department of Physiology, Tufts University School of Medicine,
Boston, MA
1988­1995 Associate Professor, Department of Radiation Oncology, Virginia Commonwealth
University (VCU), Richmond, VA
1990­1996 Section Chief, Research Section, Department of Radiation Oncology, VCU, Richmond, Virginia
1989­1995 Associate Professor, Department of Microbiology and Immunology, VCU, Richmond, VA
1995­present Professor, Departments of Radiation Oncology and Microbiology/Immunology, VCU,
Richmond VA
2004­present Professor of Physics, VCU, Richmond, VA
2007­present Chairman, Division of Molecular Radiobiology, VCU, Richmond, VA
Other Experience and Professional Memberships
1992­1994 Malaria Study Section, U.S. Agency for International Development
2001­2003 Radiation Oncology Review Panel, DOD Breast Cancer Program
2004­2006 Radiation Oncology Review Panel, DOD Prostate Cancer Program
2007­ Co­Chairman, Physical Imaging Review Panel, DOD Prostate Cancer Program
2005 Member, NIAID Study Section on Radiation Countermeasures
2006 Member, NIAID ARS Medical Countermeasures Review Committee
2007 Member, NCI Study Section, Lung Cancer and Inflammation
2007 Member, 2008/01 NCI­Subcommittee F ­ Manpower & Training
2008­ Member, 2008 VA Oncology Subcommittee
Honors
1973­1975 Fellow, Damon Runyon­Walter Winchell Memorial Cancer Foundation
1975­1976 Fellow, National Cancer Institute

B. Selected Peer­Reviewed Publications (in chronological order)
1. Mikkelsen, R. B., Stedman, T., Schmidt­Ullrich, R., and Lin, P. S. Effects of tamoxifen and protein kinase C
inhibitors on hyperthermic cell killing. Int J Radiat Oncol Biol Phys, 20: 1039­1045, 1991.
2. Mikkelsen, R. B. and Gentry, C. A radiation­induced inhibitor of chromosome condensation and nuclear
envelope breakdown in HeLa cells. Radiat Res, 132: 158­161, 1992.
3. Todd, D. G. and Mikkelsen, R. B. Ionizing radiation induces a transient increase in cytosolic free [Ca 2+ ] in
human epithelial tumor cells. Cancer Res, 54: 5224­5230, 1994.
4. Grant, S., Freemerman, A. J., Gregory, P. C., Martin, H. A., Turner, A. J., Mikkelsen, R., Chelliah, J.,
Yanovich, S., and Jarvis, W. D. Induction of apoptotic DNA fragmentation and c­jun downregulation in human
myeloid leukemia cells by the permeant Ca 2+ chelator BAPTA/AM. Oncol Res, 7: 381­392, 1995.
5. Gatmaitan, Z., Varticovski, L., Ling, L., Mikkelsen, R., Steffan, A. M., and Arias, I. M. Studies on fenestral
contraction in rat liver endothelial cells in culture. Am J Pathol, 148: 2027­2041, 1996.
6. Rao, A. S., Freemerman, A. J., Jarvis, W. D., Chelliah, J., Bear, H. D., Mikkelsen, R., and Grant, S. Effect
of AS101 on bryostatin 1­mediated differentiation induction, cell cycle arrest, and modulation of drug­induced
apoptosis in human myeloid leukemia cells. Leukemia, 10: 1150­1158, 1996.
7. Schmidt­Ullrich, R. K., Mikkelsen, R. B., Dent, P., Todd, D. G., Valerie, K., Kavanagh, B. D., Contessa, J.
N., Rorrer, W. K., and Chen, P. B. Radiation­induced proliferation of the human A431 squamous carcinoma
cells is dependent on EGFR tyrosine phosphorylation. Oncogene, 15: 1191­1197, 1997.
8. Carter, S., Auer, K. L., Reardon, D. B., Birrer, M., Fisher, P. B., Valerie, K., Schmidt­Ullrich, R., Mikkelsen,
R., and Dent, P. Inhibition of the mitogen activated protein (MAP) kinase cascade potentiates cell killing by low
dose ionizing radiation in A431 human squamous carcinoma cells. Oncogene, 16: 2787­2796, 1998.
9. Kavanagh, B. D., Dent, P., Schmidt­Ullrich, R. K., Chen, P., and Mikkelsen, R. B. Calcium­dependent
stimulation of mitogen­activated protein kinase activity in A431 cells by low doses of ionizing radiation. Radiat
Res, 149: 579­587, 1998.
10. Contessa, J. N., Reardon, D. B., Todd, D., Dent, P., Mikkelsen, R. B., Valerie, K., Bowers, G. D., and
Schmidt­Ullrich, R. K. The inducible expression of dominant­negative epidermal growth factor receptor­CD533
results in radiosensitization of human mammary carcinoma cells. Clin Cancer Res, 5: 405­411, 1999.
11. Reardon, D. B., Contessa, J. N., Mikkelsen, R. B., Valerie, K., Amir, C., Dent, P., and Schmidt­Ullrich, R. K.
Dominant negative EGFR­CD533 and inhibition of MAPK modify JNK1 activation and enhance radiation
toxicity of human mammary carcinoma cells. Oncogene, 18: 4756­4766, 1999.
12. Todd, D. G., Mikkelsen, R. B., Rorrer, W. K., Valerie, K., and Schmidt­Ullrich, R. K. Ionizing radiation
stimulates existing signal transduction pathways involving the activation of epidermal growth factor receptor
and ERBB­3, and changes of intracellular calcium in A431 human squamous carcinoma cells. J Recept Signal
Transduct Res, 19: 885­908, 1999.
13. Tombes, R. M., Mikkelsen, R. B., Jarvis, W. D., and Grant, S. Downregulation of delta CaM kinase II in
human tumor cells. Biochim Biophys Acta, 1452: 1­11, 1999.
14. Schmidt­Ullrich, R. K., Dent, P., Grant, S., Mikkelsen, R. B., and Valerie, K. Signal transduction and cellular
radiation responses. Radiat Res, 153: 245­257, 2000.
15. Bowers, G., Reardon, D., Hewitt, T., Dent, P., Mikkelsen, R. B., Valerie, K., Lammering, G., Amir, C., and
Schmidt­Ullrich, R. K. The relative role of ErbB1­4 receptor tyrosine kinases in radiation signal transduction
responses of human carcinoma cells. Oncogene, 20: 1388­1397, 2001.

16. Lammering, G., Hewit, T. H., Hawkins, W. T., Contessa, J. N., Reardon, D. B., Lin, P. S., Valerie, K., Dent,
P., Mikkelsen, R. B., and Schmidt­Ullrich, R. K. Epidermal growth factor receptor as a genetic therapy target for
carcinoma cell radiosensitization. J Natl Cancer Inst, 93: 921­929, 2001.
17. Lammering, G., Valerie, K., Lin, P. S., Mikkelsen, R. B., Contessa, J. N., Feden, J. P., Farnsworth, J., Dent,
P., and Schmidt­Ullrich, R. K. Radiosensitization of malignant glioma cells through overexpression of
dominant­negative epidermal growth factor receptor. Clin Cancer Res, 7: 682­690, 2001.
18. Leach, J. K., Van Tuyle, G., Lin, P. S., Schmidt­Ullrich, R., and Mikkelsen, R. B. Ionizing radiation­induced,
mitochondria­dependent generation of reactive oxygen/nitrogen. Cancer Res, 61: 3894­3901, 2001.
19. Leach, J. K., Black, S. J., Schmidt­Ullrich, R. K., and Mikkelsen, R.B. Activation of constitutive nitric oxide
synthase activity as an early signaling event induced by ionizing radiation. J Biol Chem, 277: 15400­15406,
2002.
20. Contessa, J. N., Hampton, J., Lammering, G., Mikkelsen, R. B., Dent, P., Valerie, K., and Schmidt­Ullrich,
R. K. Ionizing radiation activates Erb­B receptor dependent Akt and p70 S6 kinase signaling in carcinoma cells.
Oncogene. 21: 4032­4041, 2002.
21. Ho, K. C., Leach, J. K., Eley, K., Mikkelsen, R.B., and Lin, P. S. A simple method of producing low oxygen
conditions with oxyrase for cultured cells exposed to radiation and tirapazimine. Am J Clin Oncol, 26, E86­E91,
2003.
22. Mikkelsen, RB and Wardman, P. Biological chemistry of reactive oxygen and nitrogen and radiationinduced
signal transduction mechanisms. Oncogene Reviews, 22, 5734­5754, 2003.
23. Amorino GP, Mikkelsen RB, Valerie K, and Schmidt­Ullrich RK. Dominant­negative CREB inhibits
proliferating cell nuclear antigen and DNA repair, leading to increased cellular radiosensitivity. J. Biol. Chem.
278(32), 29394­29399, 2003.
24. Mikkelsen, RB. Redox signaling mechanisms and radiation­induced bystander effects. Human and
Experimental Toxicology, 23, 75­81, 2004.
25. Lammering G, Hewit TH, Holmes M, Valerie K, Hawkins W, Lin PS, Mikkelsen RB, Schmidt­Ullrich RK.
Inhibition of the Type III Epidermal Growth Factor Receptor Variant Mutant Receptor by Dominant­Negative
EGFR­CD533 Enhances Malignant Glioma Cell Radiosensitivity. Clin Cancer Res. 10(19), 6732­6743, 2004.
26. Sturla L­M, Amorino GP, Alexander MS, Mikkelsen RB, Valerie K and Schmidt­Ullrich RK. Requirement of
Tyr992 and Tyr1173 in phosphorylation of the epidermal growth factor receptor by ionizing radiation and
modulation by SHP2, J. Biol. Chem. 280, 14597­604, 2005
27. Barrett, DM, Black SM, Todor H, Schmidt­Ullrich RK, and Mikkelsen, RB. Inhibition of Protein Tyrosine
Phosphatases by Mild Oxidative Stresses is Dependent on S­Nitrosylation, J. Biol. Chem. 280, 14453­14461,
2005.
28. Contessa JN, Abell A, Mikkelsen RB, Valerie K, Schmidt­Ullrich RK. Compensatory ErbB3/c­Src signaling
enhances carcinoma cell survival to ionizing radiation. Breast Cancer Res Treat. Nov 3, 1­11, 2006.
29. Yakovlev VA and Mikkelsen RB A role for CaM­kinases in the cellular response to oxidative stress? Cancer
Biol Ther 5, 1031­2, 2006.
30. Yakovlev VA, Barani IJ, Rabender CS, Black SM, Leach KJ and Mikkelsen RB, Tyrosine Nitration of IkBa:
A novel mechanism for NF­kB activation, Biochemistry, 46 (42), 11671 ­11683, 2007.

C. Research Support
Ongoing Research Support
NIH/NCI, 5R01CA65896­11 (Mikkelsen) 03/01/1995 – 02/28/09
Radiation, ErbB activation and cellular growth control
The major goals of this project are studying the role of protein tyrosine kinase activation of EGFR as an
immediate early response to radiation and growth regulatory pathways and cellular radiosensitivity.
Role: PI
NIH/NCI T32 CA113277­01, (Mikkelsen) 07/01/07­06/30/12
Training in Radiation Oncology Translational Research
Training for 3 post­docs and 1 pre­doc in radiation oncology research.
Role: PI
NIH/NCI, 5RO1CA 90881­05 (Mikkelsen) 07/01/02­06/30/13
Radiation­induced Nitric Oxide and Cellular Radiosensitivity
The major goals of this project are to identify and characterize nitric oxide dependent
sensing/amplification mechanisms of cytoplasmic ionization events.
Role: PI