Precellys DNA Kits - Peqlab

Precellys RNA & DNA Kits
Optimised system including
homogenisation tubes plus
nucleic acid isolation kit
Highly reproducible and high
yielding results
Specially formulated protocols for
a wide range of starting materials
Ideal for use on the
Precellys ® 24 Homogeniser
ORDERING INFORMATION • Precellys RNA & DNA Kits
Introduction
Effective homogenisation 'opens up'
the test material while effective iso lation
purifies the target molecules by removing
contaminants. So the com bination of
a fully optimised and matching set of
approaches – one for homogenisation
and one for purification – maximises
yield, the reproducibility and the quality
of your downstream method.
This is the approach used in the
Precellys RNA & DNA kits, carefully
selected optimised tubes with ceramic,
steel or glass beads and purification kits
redesigned from scratch to get the very
best from your samples, all supplied as
a single, easy to use kit. Used with the
Precellys, there is no risk of cross contamination
and with very rapid protocols
these kits represent a great 'first
step' in many studies.
Precellys ® homogenisation
plus nucleic acid isolation
The key to all of this is optimisation;
optimisation in sample type, volume
and throughput. The Precellys ® 24 can
accommodate up to 24 x 2 ml tubes
and the Precellys ® Dual can accommodate
2 ml tubes or 7 ml tubes.
The selection of ceramic or glass beads
(or a combination of more than one)
in 2 ml-tubes is matched to the sample
type to bring even the toughest of materials
into fine particles. The control of
the system enables the sample to be
homogenised to release nucleic acids
but not destroy or damage them.
The complementary nucleic acid isolation
kits combines the homogenisation
and lysis in just one step, shortening
protocol times and avoiding sample
handling. The lysate is passed through
the high yielding PerfectBind ® silica columns
which, through centrifugation and
washing, are cleared of proteins, polysaccharides
and unwanted materials
before the high purity DNA or RNA is
eluted into RNase free water or elution
buffer ready for downstream use.
The outcome – shorter working times,
less sample handling, less waste and
more reliable and stable downstream
work.
Further information on the Precellys ®
homogenisers can be found on
www.peqlab.com
Telefon +49 (0) 9131 - 610 70 20 · Telefax +49 (0) 9131 - 610 70 99 Freecall(D) 0800 - 100 20 16 · Freefax(D) 0800 - 100 90 16

Precellys DNA Kits
For the homogenisation and DNA
purification from soil, microorganisms,
animal or plant material
Reproducible and effective results
Works in conjunction with the
Precellys homogeniser and other
'bead beating' instruments
M 1 2 3
A
B
Agarose gel electrophoresis (1 %, TAE) of
DNA purified using Precellys DNA kits
(M = DNA Sizer II).
A) DNA purified from soil using the
Precellys Soil DNA kit.
B) DNA from yeast purified using the
Precellys Bacterial/Fungal DNA kit.
C) DNA from pig ear purified using
the Precellys Tissue DNA kit.
D) DNA from Arabidopsis leaves
purified using the Precellys
Plant DNA kit.
M 1 2 3
ORDERING INFORMATION • Precellys DNA Kits
C
D
Features
- Supplied with optimised selection of
homogenisation beads
- Free from organic extraction and time
consuming alcohol precipitation
- 'Safety Line' columns with caps remove
risk of sample contamination
Method
Precellys DNA kits use the reversible
binding characteristics of PerfectBind ®
silica centrifugation tubes for rapid and
effective sample processing. Samples
are first homogenised and lysed and
then passed through the silica column.
DNA selectively binds to the silica
matrix and, after washing, is eluted in
elution buffer or water ready for downstream
use.
PRECELLYS SOIL DNA KIT
- For the isolation of high purity DNA
from soil and mixed material samples.
- Particularly well suited for the isolation
of DNA from difficult to lyse microorganisms
- Kit includes Precellys glass and
ceramic bead tubes (SK38).
- For up to 250 mg sample per column
PRECELLYS BACTERIAL/FUNGAL
DNA KIT
- Designed for difficult to lyse
mushrooms, yeasts, gram + bacteria
and spores
- Kit includes Precellys 0.5 mm glass
bead tubes for effective sample
homogenisation prior to purification
- For up to 100 mg mushroom material,
1 x 10 8 yeast cells or 1 x 10 9 bacteria
per column
PRECELLYS TISSUE DNA KIT
- Ideal for tough, resistant tissues like
tumour biopsies, cartilage and
connective tissue
- Kit includes Precellys 1.4/2.8 mm
ceramic beads for effective tissue
homogenisation
- For up to 30 mg animal tissue
PRECELLYS PLANT DNA KIT
- Ideal for plant stems, roots and leaves
- Contains microfilter columns for
efficient removal of debris
- Kit includes Precellys 1.4/2.8 mm
ceramic beads for effective homogenisation
- For up to 100 mg plant tissue
material
A total methodology from starting
sample to purified DNA.
PRODUCT DESCRIPTION YIELD PREPS CAT. NO.
Precellys Soil DNA Kit
(Safety Line)
Precellys Bacterial/Fungal
DNA Kit (Safety Line)
Precellys Tissue DNA Kit
(Safety Line)
Precellys Plant DNA Kit
(Safety Line)
DNA from up to 250 mg soil up to 30 µg 5 12-7501-00
50 12-7501-01
200 12-7501-02
DNA from up to 100 mg material
up to 30 µg 5 12-7511-00
or 1 x 10 9 cells
50 12-7511-01
200 12-7511-02
DNA from up to 30 mg animal tissue up to 50 µg 5 12-7521-00
50 12-7521-01
200 12-7521-02
DNA from up to 100 mg plant material up to 30 µg 5 12-7531-00
50 12-7531-01
200 12-7531-02
Telefon +49 (0) 9131 - 610 70 20 · Telefax +49 (0) 9131 - 610 70 99 Freecall(D) 0800 - 100 20 16 · Freefax(D) 0800 - 100 90 16