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RNA Guide: Purifying RNA and mRNA - Promega

RNA Guide: Purifying RNA and mRNA - Promega

RNA Guide: Purifying RNA and mRNA - Promega

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<strong>Purifying</strong> <strong>RNA</strong> <strong>and</strong> m<strong>RNA</strong><br />

Cultured<br />

Cells<br />

Unique product!<br />

Novel clearing agent<br />

removes all detectable<br />

genomic DNA.<br />

No DNase<br />

treatment needed!<br />

Automated<br />

Manual<br />

MagneSil ® Total <strong>RNA</strong><br />

mini-Isolation System<br />

SV 96 Total <strong>RNA</strong><br />

Isolation System<br />

PureYield <strong>RNA</strong><br />

Midiprep System<br />

SV Total <strong>RNA</strong><br />

Isolation System<br />

Can process: ~10 5<br />

cells in 96-well<br />

format; s10 3 cells in<br />

384-well format<br />

Precipitation of <strong>RNA</strong> Samples<br />

DNase included to<br />

greatly reduce<br />

gDNA carry-over.<br />

Precipitation of <strong>RNA</strong> is commonly used for purification<br />

or concentration purposes. To ensure maximum<br />

recovery when precipitating small quantities (

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