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Asian J. Pharm. Ana. 2012; Vol. 2: Issue 4, Pg 114-115
from light. From this stock solution, a series of standards
were freshly prepared during the analysis day 3 .
ASSAY OF CHOLINE BITATRATE IN TABLET
FORMULATION
Working Standard Solution:
Absorbance
0.08
0.06
0.04
0.02
0
Graph no.1 Standard curve for choline Bitartrate
by U.V Spectrophotometric Method
5 10 15 20 25 30 35
Concentration mcg/ml
A standard stock solution was prepared by dissolving 100
mg of Choline Bitartrate in 100 ml water, and further
diluted with same solvent to get 100 µg/ml.
Preparation of Sample Solution:
Ten tablets were weighed, and then powdered sample of the
powdered tablets, equivalent to 75mg of the active
ingredient was weighed and dissolve in 100ml of purified
water and further diluted with water to get 75 µg/ml. This
experiments was repeated 5 times for tablet formulation to
find the drug content in each tablet. Readings were taken
and shown in Table no.1.
[AJPAna.]
Recovery Studies:
To study the accuracy and reproducibility of the UV
method, recovery experiments were carried out. The
recovery experiments were performed for tablet
formulation. A known volume of standard stock solution
was added to the flask containing pre analyzed sample
solution. The recovery of added standard drug was studied
at three different levels. Each level was repeated five times.
Readings were taken and shown in Table no. 2.
RESULTS AND DISCUSSION:
UV Scan of Choline Bitartrate in bulk Showed λmax value
at 208nm in 10 µg/ml concentration. Beers range were
performed to obtain linearity between 5-35 µg/ml, results
were shown in Graph no.1. Assay of Choline Bitartrate
using UV Spectrophotometry at maximum absorbance at
208 nm gives precise and accurate results for amount
present in tablet formulation.
CONCLUSIONS:
The proposed method for Choline Bitartrate have many
advantages over other analytical methods due to its lower
cost, convenient method and environmental safety.
Economically compared to other analytical methods, this
method can be extended for the routine assay of Choline
Bitartrate and its formulations. The low value of standard
deviation and co efficient of variation indicates that the
proposed method is accurate, precise and reproducible.
Table no.1 Assay of Choline Bitartrate in Tablet Formulation
Sl. No. Weight of sample taken
(mg)
Absorbance Amount present in
mg/tablet
% of drug
content
1 156.9 0.1926 540 98.1%
2 157.0 0.1928 540 98.1%
3 157.2 0.1931 541 98.3%
4 157.3 0.1931 540 98.1%
5 157.5 0.1934 541 98.3%
Standard
division
Co efficient of
variance
0.547 98.1%±0.10%
Table no.2 Recovery Studies
Sl. No. Formulation Amount of Standard added Amount Found % Recovery
1 Sample 8.0mg 7.5mg
2 Sample 15.0mg 15.0mg
94
3 Sample 28.0mg 22.5mg
ACKNOWLEDGEMENT:
The authors are thankful to the Founder Chairperson of
Periyar College of Pharmaceutical Sciences, Trichy for
extending Laboratory facilities to carry out this work.
REFERENCES:
1. Huang T et al ; Liq chromatography B 670 : 323 – 327 , 1995.
2. K Lein J et al ; Neurochem Int 22:293 – 300 , 1993.
3. Heilbronn E, and Carrisson, B., J. Chromatogr. 4, 257 – 259,
1960.
4. Kneczke M. J. Chromatogr 1980.
5. USP 30 – NF 25, 905 Pharmacopeial Forum No.30 (3) 950.
115