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Asian J. Pharm. Ana. 2012; Vol. 2: Issue 4, Pg 114-115
[AJPAna.]
ISSN- 2231–5667 (Print)
ISSN- 2231–5675 (Online)
www.asianpharmaonline.org
RESEARCH ARTICLE
Spectrophotometric method of Choline Bitartrate in bulk and its tablet
formulation
G. Krishnamoorthy*, C. Diana Priyadarshini, R. Senthamarai
Department of Pharmaceutical Analysis, Periyar College of Pharmaceutical Sciences, Tiruchirappalli – 620 021.
*Corresponding Author E-mail: vkm292011@hotmail.com
ABSTRACT:
A Simple, precise, rapid and accurate UV spectrophotometric method was developed for the estimation of Choline
Bitartrate in bulk and its tablet formulation. UV scan of Choline Bitartrate showed λmax (absorbance) value at 208 nm
in the concentration range of 10 µg/ml. This method is based on the measurement of light absorption in the UV region
using water as solvent. Beer’s Law plot was constructed by measuring the absorbance at 208 nm using various
concentration of solution 5-35 µg/ml and show linearity and limit of detection. A simple and convenient method is
applied to the determination of amount of choline bitartrate present in tablet formulation with high percentage of
recovery, good accuracy and precision. The results are found satisfactory and reproducible. The proposed method was
successfully extended to pharmaceutical preparation tablet.
KEYWORDS: Choline Bitartrate, UV spectrophotometry absorption maxima, Pharmaceutical formulation, Beer’s
range, Recovery studies.
INTRODUCTION:
Choline Bitartrate is involved in variety of intricate
biochemical reactions involving multiple systems of the
body, including the brain, musclas and internal organs.
Specifically, we need choline in order to form healthy cell
membranes, breakdown of cholesterol, and aid in the
function of neurotransmitters in the brain 1-5 . Chemically it is
(2- Hydroxyethyl) trimethylammonium – L – (+) – tartrate
salt (Fig.1). Its molecular weight is 253.25 and molecular
formula is C 9 H 19 NO 7 . Literature survey reveals no ultra
violet spectrophotometry method developed for the
estimation of choline Bitartrate in bulk and its tablet
formulation. The aim of the study was to develop a simple,
precise and accurate spectrophotometric method for the
estimation of choline bitartrate in bulk and its tablet
formulation. Several analytical techniques like HPLC, Nonaqueous
titration have been reported for routine analysis. So
the authors have made some attempts in developing UV
spectrophotometric method.
Received on 29.08.2012 Accepted on 20.10.2012
© Asian Pharma Press All Right Reserved
Asian J. Pharm. Ana. 2(4): Oct. - Dec. 2012; Page 114-115
CHEMICAL STRUCTURE 2
Fig.1 Chemical structure of choline bitartrate
MATERIALS AND METHODS:
Apparatus and Chemicals:
A Shimadzu UV–Visible spectrophotometer 1601 with 1cm
matched quartz cells was used for all spectral
measurements. All chemicals were of analytical reagent
grade and solutions were prepared with purified water
pharmaceutical grade of choline Bitartrate was gifted by
Goodman pharmaceuticals, Puducherry, India.
Absorption Spectra of Choline Bitartrate in Water
About 100mg of choline bitrartrate in pure was dissolved in
100 ml of water to get 1 mg/ml stock solution. It was
diluted with same solvent to get 100 µg/ml. and it was
further diluted with same solvent to get 10 µg/ml. The
prepared stock solution was stored at dark place protected
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Asian J. Pharm. Ana. 2012; Vol. 2: Issue 4, Pg 114-115
from light. From this stock solution, a series of standards
were freshly prepared during the analysis day 3 .
ASSAY OF CHOLINE BITATRATE IN TABLET
FORMULATION
Working Standard Solution:
Absorbance
0.08
0.06
0.04
0.02
0
Graph no.1 Standard curve for choline Bitartrate
by U.V Spectrophotometric Method
5 10 15 20 25 30 35
Concentration mcg/ml
A standard stock solution was prepared by dissolving 100
mg of Choline Bitartrate in 100 ml water, and further
diluted with same solvent to get 100 µg/ml.
Preparation of Sample Solution:
Ten tablets were weighed, and then powdered sample of the
powdered tablets, equivalent to 75mg of the active
ingredient was weighed and dissolve in 100ml of purified
water and further diluted with water to get 75 µg/ml. This
experiments was repeated 5 times for tablet formulation to
find the drug content in each tablet. Readings were taken
and shown in Table no.1.
[AJPAna.]
Recovery Studies:
To study the accuracy and reproducibility of the UV
method, recovery experiments were carried out. The
recovery experiments were performed for tablet
formulation. A known volume of standard stock solution
was added to the flask containing pre analyzed sample
solution. The recovery of added standard drug was studied
at three different levels. Each level was repeated five times.
Readings were taken and shown in Table no. 2.
RESULTS AND DISCUSSION:
UV Scan of Choline Bitartrate in bulk Showed λmax value
at 208nm in 10 µg/ml concentration. Beers range were
performed to obtain linearity between 5-35 µg/ml, results
were shown in Graph no.1. Assay of Choline Bitartrate
using UV Spectrophotometry at maximum absorbance at
208 nm gives precise and accurate results for amount
present in tablet formulation.
CONCLUSIONS:
The proposed method for Choline Bitartrate have many
advantages over other analytical methods due to its lower
cost, convenient method and environmental safety.
Economically compared to other analytical methods, this
method can be extended for the routine assay of Choline
Bitartrate and its formulations. The low value of standard
deviation and co efficient of variation indicates that the
proposed method is accurate, precise and reproducible.
Table no.1 Assay of Choline Bitartrate in Tablet Formulation
Sl. No. Weight of sample taken
(mg)
Absorbance Amount present in
mg/tablet
% of drug
content
1 156.9 0.1926 540 98.1%
2 157.0 0.1928 540 98.1%
3 157.2 0.1931 541 98.3%
4 157.3 0.1931 540 98.1%
5 157.5 0.1934 541 98.3%
Standard
division
Co efficient of
variance
0.547 98.1%±0.10%
Table no.2 Recovery Studies
Sl. No. Formulation Amount of Standard added Amount Found % Recovery
1 Sample 8.0mg 7.5mg
2 Sample 15.0mg 15.0mg
94
3 Sample 28.0mg 22.5mg
ACKNOWLEDGEMENT:
The authors are thankful to the Founder Chairperson of
Periyar College of Pharmaceutical Sciences, Trichy for
extending Laboratory facilities to carry out this work.
REFERENCES:
1. Huang T et al ; Liq chromatography B 670 : 323 – 327 , 1995.
2. K Lein J et al ; Neurochem Int 22:293 – 300 , 1993.
3. Heilbronn E, and Carrisson, B., J. Chromatogr. 4, 257 – 259,
1960.
4. Kneczke M. J. Chromatogr 1980.
5. USP 30 – NF 25, 905 Pharmacopeial Forum No.30 (3) 950.
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