Autologous Bone Marrow transplantation - Blog Science Connections

688 Peripheral Blood CFU-GM and BFUe
formation was completely inhibited when MO exceeded 7 x 10 5 /ml
(9). Other laboratories have observed the stimulatory effects of T cells
(12) and the inhibitory influence of MO (19-21). This study shows
that the ratio of T cells to monocytes (as well as the absolute
concentrations) strongly affects CFU-GM cloning efficiency.
T cell stimulation was initially demonstrated with LCM as a source
of CSA (9). In this study, comparable stimulation by T cells was seen
with 2 other CSA sources, GCT and 5637CM (Table 1A). T cells
enhanced progenitor growth to similar extents with all 3 CM. In each
case, the CM was used at its optimal concentration. LCM and 5637CM
supported CFU-GM formation better than GCT (p < 0.05), regardless
of whether T cells were present in the culture system. Mixing 2
different CM at their optimal concentrations did not yield as many
CFU-GM as did 1 CM supplemented with T cells (Table IB). The
number of CFU-GM in 2 CM were not different from those cultured
Table 1A. Stimulation of CFU-GM Growth by
Autologous T-Cells in 1 or 2 CM a
B/Null Cells Cultured in a Single CM
LCM 5637CM GCT
B/Null 1.00 0.84±0.08(10) 0.72±0.05 b (9)
+ T cells
B/Null 0.44±0.10 c (12) 0.38±0.07 c (12) 0.22±JJ.07 C (10)
Table IB. Stimulation of CFU-GM Growth by
Autologous T Cells in 1 or 2 CM a
B/Null Cells Cultured with Combinations of 2 CM
- T Cells + T Cells
LCM & GCT
LCM & 5637CM
5637CM & GCT
0.32+0.10 (9)
0.43±0.11 (12)
0.38±0.09 (9)
0.86±0.10 (8)
1.13±0.12 (12)
0.81±0.12 (8)
a
Relative cloning efficiency of CFU-GM grown with T cells to that without T cells is
shown in the table. The number of CFU-GM in LCM with added autologous T cells
was arbitrarily defined as 1.0 for each donor to facilitate comparison. An average of
36.5 + 20.3 (12) CFU-GM were observed in 5 x 10 4 B/Null cells. The number of
independent experiments is given in brackets.
^Significantly different (p < 0.05) from the LCM value with T cells.
c
Significantly different (p < 0.05) from the corresponding values in the same CM
without T cells.