Proper Pipette Technique Proper Pipette Technique

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Pipette Calibration Frequency of Calibration • It is recommended to calibrated pipettes every 3 months. Single Channel Pipette Calibration Procedure • Use an analytical calibrated balance with an accuracy of 0.0001g. • Use non-aerated distilled or deionized water. • Perform calibration in a draft free room or with an enclosed balance. • Maintain the relative humidity above 55% to decrease evaporation effects. Air humidity should be as high as possible especially for volumes below 50µL. © EnviroLogix 2006
Pipette Calibration Single Channel Pipette Calibration Procedure: • Pipette, water, and air should be the same temperature, between 15-30C. • Test maximum pipette volume and minimum volume or 10% of maximum volume (which ever is greater). • Pre-wet tips. • Test 10 volumes per setting. Multi-channel Pipette Calibration Procedure: • Use single channel procedure for all 8 or 12 channels. © EnviroLogix 2006
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Proper Pipette Technique Proper Pipette Technique

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