Proper Pipette Technique Proper Pipette Technique

General Pipette Technique
Manufacturer Recommendations
In order of importance:
1. Wet pipette tip 2-3 times with working solution
before initiating work. Pre-wetting increase the
humidity within the tip and decreases sample
evaporation. Using the same tip without pre-wetting
to deliver multiple samples will result in a lower
volume for the first few samples.
2. Make sure pipettes, tips, and solutions are at the
same temperature. The volume of sample delivered
by air displacement pipettes varies with air
pressure,relative humidity, and vapor pressure.
Working with all components at a constant
temperature minimizes variation.
© EnviroLogix 2006