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Hook Effect in ELISA<br />

• The antibody coated on the plate and conjugate<br />

antibody have their antigen binding sites occupied<br />

so that the antigen can not be sandwiched between<br />

the two antibodies.<br />

Hook Effect Sandwich Assay:<br />

Bt<br />

Bt<br />

Bt<br />

Bt<br />

Bt<br />

Bt<br />

Bt<br />

Bt<br />

Bt<br />

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Bt<br />

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Bt<br />

Bt<br />

Bt<br />

Bt<br />

Bt<br />

Bt<br />

Bt<br />

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