Proper Pipette Technique Proper Pipette Technique

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Reverse vs. Forward Pipetting Reverse Pipetting: • For high viscosity solutions, small volumes, buffers with detergent, and solutions that foam easily. • Depress push button all the way to the second stop. • Place the tip 1 cm below the surface of the liquid and draw solution slowly up. Withdraw tip touching it against the sides of the reservoir to remove excess liquid. • Deliver solution by depressing the push button to the first stop. A portion of the solution will remain in the pipette tip. This volume should not be delivered. © EnviroLogix 2006
Positive Displacement vs. Air Displacement <strong>Pipette</strong>s Air Displacement: • Precise draining by air pressure (air cushion) • For normal solutions and buffers Positive Displacement <strong>Pipette</strong>s: • For viscous, dense, volatile, radioactive or corrosive samples • Direct contact of samples with piston • Contains an internal mechanism for wiping off pipette barrel © EnviroLogix 2006
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Page 3: Reverse vs. Forward Pipetting Forwa
Page 7 and 8: General Pipette Technique Manufactu
Page 13 and 14: Pipette Calibration and Cleaning Ja
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Proper Pipette Technique Proper Pipette Technique

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