Starch Gel Electrophoresis Techniques

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Applications Know seed quality before conditioning and delivering seed to customer Less than 2 week, vs. grow outs Improve inbred uniformity Verify varieties Can test brace roots from field samples to help identify problems
Applications Breeder’s seed Eliminate segregating lines If 2 loci are segregating, could develop 4 new homozygous lines: ACP1: 2/2,2/4,4/4 MDH2: 3/3,3/6,6/6 Type A: ACP1=2/2; MDH2=3/3 Type B: ACP1=2/2; MDH2=6/6 Type C: ACP1=4/4; MDH2=3/3 Type D: ACP1=4/4; MDH2=6/6
Page 1 and 2:
Starch Gel Electrophoresis Techniqu
Page 3 and 4:
What is Starch Gel Isozyme Electrop
Page 5 and 6:
Reference Manual Contents Equi
Page 7 and 8:
Maize Chromosome Map Each gene had
Page 9 and 10:
“B” Gel Ingredients L-histidine
Page 11 and 12:
Gel Mold
Page 13 and 14: Gel Mold—Taping
Page 15 and 16: Pouring the Gel
Page 17 and 18: Harvesting Coleoptile Tissue REPRES
Page 19 and 20: Extraction Buffer Releases protein
Page 21 and 22: Use to crush seedlings Crusher
Page 23 and 24: Paper Wick
Page 25 and 26: Samples From One Seed Lot
Page 27 and 28: Cut off meniscus Leveling Gel Top
Page 29 and 30: Inset wick— section 1 Loading Gel
Page 31 and 32: Gel loading completed; insert marke
Page 33 and 34: Ohm’s Law I=E/R Electric curre
Page 35 and 36: Place gel mold in buffer trays Runn
Page 37 and 38: Set upper limit for desired constan
Page 39 and 40: After Electrophoresis origin Gel ac
Page 41 and 42: Running the Gel Need to keep the g
Page 43 and 44: Slicing the Gel Additional stains.
Page 45 and 46: Removing gel from mold
Page 47 and 48: Draw wire through gel Slicing the G
Page 49 and 50: Draw wire through gel again; repeat
Page 51 and 52: Place gel slice in staining tray Tr
Page 53 and 54: Staining the Gel Reveals zones of e
Page 55 and 56: Stained Gel Slice MDH Malate dehydr
Page 57 and 58: Why Corn? Highly polymorphic (m
Page 59 and 60: Other Crops Canola PHI ACO Sunflo
Page 61 and 62: PROS Quick turn around time
Page 63: Field vs Lab test Variant or offty
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Starch Gel Electrophoresis Techniques

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