CURRICULUM VITAE of GIUSEPPE CHIRICO Born 14th ... - Infn

CURRICULUM VITAE of GIUSEPPE CHIRICO
Born 14th August 1963 in Asmara (Eritrea); Italian citizenship.
email: giuseppe.chirico@mib.infn.it
Affiliation: Department of Physics, University of Milano Bicocca. Piazza della Scienza 3, 20126
Milano (I). tel. -39-0264482440; Lab: -39-0264482520; Fax: -39-0264482447
Education: Degree in Physics: 1986, University of Milano; Doctorate in Biophisics: 1990
University of Milano
Scientific Activity. GC has employed laser spectroscopy and microscopy to investigate the
structure and dynamics of proteins and DNA in vitro and in vivo. To this purpose he is using
currently Fluorescence Correlation Spectroscopy and Fluorescence Lifetime and Polarization
Anisotropy to study single proteins in cells and in inert matrices. The most recent results are on
mutants of Green Fluorescent Protein in silica gels, in polyelectrolyte Nanocapsules and in cells.
His main expertise is in time resolved and correlated fluorescence and confocal and two-photon
microscopy also with application to in-vivo studies.
Positions. 1990 Postdoc at the European Molecular Biology Laboratory (EMBL), Grenoble
Outstation (France); 1991 NATO--CNR Fellowship at the European Molecular Biology
Laboratory (EMBL), Grenoble Outstation (France); 1994 Visiting Researcher at the “Deutches
KrebsForschung Zentrum” (German Cancer research center) of the Heidelberg University; 1991-
99 Researcher in Physics at the University of Milano; 1999 Visiting researcher at the
Laboaratory for Fluorescence Dynamics (LFD) at the University of Illinois at Urbana-
Champaign; 2000- 2004 Associate Professor in Physics at the university of Milano – Bicocca;
2005 – Full professor in Physics at the university of Milano - Bicocca.
Selected Publications (http://fisica.mib.infn.it/media/homepages/biofisica/bicoccahepth/index.html
for a full list).
- Bacchetta R., Santo N., Fascio U., Moschini E., Freddi S., Chirico G., Camatini M. , Mantecca P. Nanotoxicology.
Nano-sized CuO, TiO2 and ZnO affect Xenopus laevis development. doi:10.3109/17435390.2011.579634) 6:
381-398 , 2012.
- Laura Sironi, Stefano Freddi,Michele Caccia,Paolo Pozzi,Leone Rossetti,Piersandro Pallavicini, Alice
Donà,Elisa Cabrini,Maurizio Gualtieri,Ilaria Rivolta,Alice Panariti, Laura D’Alfonso, Maddalena Collini,
Giuseppe Chirico. Gold Branched Nanoparticles for Cellular Treatments. J. Phys. Chem. C: 116 (2012)
18407-18418 .
- Pallavicini P., Chirico, G., Collini,M.; D’Alfonso, L.; Denat, F.; Diaz-Fernandez, Y-A.; Pasotti, L.; Rousselin, Y.
and Sok, N. 2008. Micelles as containers for self-assembled nanodevices: simultaneous evaluation of the
lipophilicity and acidity constants of fatty acids through fluorescence. ChemPhysChem. 3, 1729 – 1737
(2008).
- Freddi, S.; D’Alfonso, L.; Collini, M., Caccia, M., Sironi, L.; Tallarida, G.; Caprioli, S.; and G. Chirico. Excited-
State Lifetime Assay for Protein Detection on Gold Colloids-Fluorophore Complexes. J. Phys. Chem. C, 113
(7), 2722-2730 (2009).
- Sironi, L., Freddi, S., D’Alfonso, L._ Collini, M., Gorletta, T., Soddu, S., and Chirico, G. p53 Detection by
Fluorescence Lifetime on a Hybrid Fluorescein Isothiocyanate Gold Nanosensor. J. Biomed. Nanotech. Vol. 5,
683–691 (2009).
- G. Chirico, F. Cannone, A. Bizzarri, S. Cannistraro, Quenching and Blinking of Fluorescence of Single Dye
Molecule Bound to Gold Nanoparticles, J. Phys. Chem. B.; 2006; 110(33); 16491-16498.
- Alberto Diaspro, Silke Krol, Barbara Campanini, Fabio Cannone, Giuseppe Chirico. Enhanced Green Fluorescent
Protein (GFP) fluorescence after polyelectrolyte caging. 16 October 2006 / Vol. 14, No. 21 / OPTICS EXPRESS
9815

- Fabio Cannone; Roberto Milani; Giuseppe chirico; Alberto Diaspro; Silke Krol; Barbara Campanini. Voltage
Regulation of Single Green Fluorescent Protein Mutants. Biophys. Chem. in press.
-Baldini G., Cannone F. and G. Chirico, Pre-Unfolding Resonant Oscillations of Single Green Fluorescent Protein
Molecules, Science , (2005) 309:1096-1100.
- B. Campanini, Bologna S, Cannone F, Chirico G, Mozzarelli A, Bettati S. Unfolding of Green Fluorescent Protein
mut2 in wet nanoporous silica gels, PROTEIN SCIENCE (2005) 14 (5): 1125-1133.
- G. Chirico, A. Diaspro,F. Cannone,M. Collini, S. Bologna, V. Pellegrini and F. Beltram. Selective Fluorescence
Recovery after Bleaching of Single E2GFP Proteins Induced by Two-Photon Excitation. ChemPhysChem (2005), 6,
328 – 335
- G. Chirico, F. Cannone, A. Diaspro, S. Bologna, V. Pellegrini, R. Nifos`ı and F. Beltram, Multi-photon switching
dynamics of single green fluorescent proteins, Phys.Rev.E, 70, 030901(R) (2004).
- G. Chirico, F. Cannone, G. Baldini, A. Diaspro, Two-photon Thermal Bleaching of Single Fluorescent Molecules,
Biophys. J. (2003) 84:588-598.
- G. Chirico, F. Cannone, S. Beretta, A. Diaspro, B. Campanini, S. Bettati, R. Ruotolo and A. Mozzarelli,
"Dynamics of Green Fluorescent Protein mutant2 in solution, on spin-coated glasses and encapsulated in
nanoporous silica gels", Protein Science, (2002) 11:1152–1161.
- G. Chirico, S. Bettati, A. Mozzarelli, Y. Chen, J. D. Müller, E. Gratton, "Molecular Heterogeneity of O-
Acetylserine Sulfhydrylase by Two-Photon Excited Fluorescence Fluctuation Spectroscopy", Biophys. J., (2001),
80: 1973-1985
- G. Chirico, S. Beretta, G.Baldini, " Conformation of interacting lysozyme by polarized and depolarized light
scattering", J.Chem. Phys., (1999) 110:2297-2304.
- G. Chirico, J.Langowski, "Brownian Dynamics Simulations of plasmid DNA with bent sequences", Biophysical
Journal, (1996) 71 955-971.
Selected oral contribution to congresses.
1. SPIE- Photonics West, San Francisco 2013. Structured illumination fluorescence correlation spectroscopy
for velocimetry in Zebrafish embryos.
2. NanoMed 2012, London. Branched Gold Nanoparticles for Thermal Treatments of Cells.
3. NANO2006, Workshop, San Sebastian (E), Voltage Regulation of the fluorescence emission of single
molecules. 09/2006.
4. Medicon 2004, Mediterranean Conference on Medical and Biological Engineering: "Health in the
Information Society", Ischia, 31-07/5-08 2004.
5. Photonics West, San Jose, 2004.
6. Focus on Microscopy: Genova 2003.
7. Single Particle Methodologies in Biophysics and Biotechnology, Workshop, Firenze, 2001.
Referee of Biophys.J.; Biochem.Phys., ChemPhysChem; FEBS Letters; Micr.Res.Techn.; J.Biom.Opt.
Editorial board of Biomedical Optical Engineering (IEEE), Cell Biology and Biophysics, PLOSONE, J. Biophysics.