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PHYSICAL EVIDENCE MANUAL - Crime Scene Investigator Network

PHYSICAL EVIDENCE MANUAL - Crime Scene Investigator Network

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Physical Evidence Manual<br />

Safety and Contamination Prevention<br />

Current DNA technology allows for very small amounts of sample to be analyzed.<br />

For example, a blood stain the size of a sharpened pencil point may be enough<br />

to perform DNA analysis on, as would the residue amount of sweat and skin cells<br />

on the inside rim of a ball cap. Because of this, inadvertent contamination of the<br />

evidence is possible if you do not take precautions.<br />

• Wear gloves and, if desired, a mask and eye protection while collecting<br />

biological samples.<br />

• Change gloves frequently or anytime your gloves are contaminated with<br />

biological material.<br />

• Do not touch the tips of cotton swabs with your fingers.<br />

• Avoid contaminating your swabs by getting them dirty, talking over them,<br />

blowing on them to make samples dry faster, etc. Consider purchasing<br />

individually wrapped sterile swabs.<br />

• Do not touch your water dropper bottle tip to any surface or evidence.<br />

• Clean tools (such as scissors or tweezers) that you might use to collect<br />

evidence with a 10% bleach solution and dry thoroughly.<br />

General Collection Guidelines<br />

In general, wet or moist biological evidence should be dried if possible and<br />

packaged into paper containers. Paper packaging prevents the evidence from<br />

degrading, so wrapping the evidence first in plastic then placing that inside paper<br />

(or vice versa) defeats the purpose. Mark the packaging with a “BIOHAZARD”<br />

label.<br />

Rules of thumb for long-term storage of biological evidence is refrigerate wet or<br />

liquid evidence and freeze dry evidence.<br />

UV Light Searches<br />

Many officers chose to use an ultraviolet (UV) light, Woods lamp, or other<br />

alternate light source to assist in the search for biological stains. Such devices<br />

can be helpful in a search, given that many biological stains such as semen and<br />

saliva may fluoresce, or appear bright, when viewed with UV light in a darkened<br />

room.<br />

However, there are three important points to remember when using a UV light to<br />

assist your search for biological stains:<br />

Adopted: May 2002<br />

Revisions: 6<br />

Last Revision: January 31, 2008<br />

6-2

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