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Sensors & Transducers Journal, Vol. 92, Issue 5, May 2008, pp. 122-133 The use of DNA biostrip and biochip technologies eliminates the role of PCR. Future biosensors will require the development of new reliable devices or the improvement of the existing ones in order to allow superior transduction, amplification, processing, and conversion of the biological signals. Efficient biosensors will not necessarily function as a stand-alone detector, but will form an integral part of an analytical system. Compact and portable devices will constitute another future area of intensive multidisciplinary sensor research. Further, increase of interest to DNA based sensors can be expected in near future together with a commercial production of these devices and their wide use. However, basic research is still necessary to improve the sensor technologies, sensing strategies as well as analytical instrumentations and procedures. Acknowledgment The author is thankful to Department of Science and Technology, Ministry of Science and Technology, Govt. of India, Delhi for funding project on DNA based biosensor to IGIB. References [1]. R. Eden-Firstenberg, B. J. Schaertel, Biosensor in the food industry: Present and Future, J. of Food Protection, 51, 1988, pp. 811-820. [2]. D. Lindner, Lab as a chip, 1, 2001, pp. 15-19. [3]. F. M. Burkle, Measures of effectiveness in large scale bioterrorism events, Prehosp Disast. Med., 18, 2003, pp. 258-262. [4]. M. Maseini, Affinity electrochemical biosensors for pollution control, Pure Appl. Chem., 73, 2001, pp. 23-30. [5]. B. D. Malhotra, A. Chaube, Biosensors for clinical diagnostics industry, Sensor Actuators B, 91, 2003, pp. 117-126. [6]. B. Annelies, B. Dirk, L. Michel, Clinical and analytical performance of the Accu-chek inform point-of-care glucose water, Poin of Care, 4, 2005, pp. 36-40. [7]. V. Anjum, C. S. Pundir, Biosensors: Future analytical tools, Sensors and Transducers, 76, 2007, pp. 937-944. [8]. R. Blonder, S. Levi, G. Tao, I. Ben-Dov, J. Willner, Development of amperometric and microgravimetric immunosensors and reversible immunosensors using antigen and photoisomerizable antigen monolayer electrodes, J. Am. Chem. Soc., 119, 1997, pp. 467- 478. [9]. R. Blonder, I. BenDov, A. Dagana, I. Willner, E. Zisman, Photochemically activated electrodes: application in design of reversible immunosensors and antibody patterned interfaces, Biosens. Bioelectron., 12, 1997, pp. 627-644. [10]. T. G. Prummond, Electrochemical DNA Sensors, Nature Biotechnol, 21, 2003, pp. 1192-1199. [11]. R. M. Umek, Electronic detection of nucleic acids, J. Mol. Diagnostics, 3, 2001, pp. 74-84. [12]. Z. Junhui, C. Hong, Y. Ruifu, DNA based biosensors, Biotech. Advanc., 15, 1997, pp. 43-58. [13]. K. Arora, S. Chand, B. D. Malhotra, Recent developments in biomolecular electronics techniques for food pathogens, Anal. Chim Acta, 568, 2006, pp. 259-274. [14]. K. Arora, N. Prabhakar, S. Chand, B. D. Malhotra, Ultrasensitive DNA hybridization biosensor based on polyaniline, Biosens. Bioelectronics, 23, 2007, pp. 613-620. [15]. N. Prabhakar, K. Arora, S. P. Singh, M. K. Pandey, H. Singh, B. D. Malhotra, Polypyrrole-polyvinyl sulphonate film based disposable nucleic acid biosensor, Anal. Chim Acta, 589, 2007, pp. 6-13. [16]. D. Berdat, A. Marin, F. Herrera, M. A. M. Gijs, DNA biosensors using fluorescence microscopy and impedance spectroscopy, Sensors Actuators, 118, 2006, pp. 53-59. [17]. S. K. Sharma, N. Sehgal, A. Kumar, Biomolecules for development of biosensors and their application, Curr. Appl. Phys., 3, 2003, pp. 307-316. [18]. K. C. Ho, C. Y. Cheu, H. C. Hsu, L. C. Cheu, S. C. Shiesh, X. Z. Lin, Amperometric detection of morphine at a prussian blue modified indium tin oxide electrode, Biosens. Bioelectron., 20, 2004, pp. 3-8. [19]. J. Wang, D. K Xu, A. N. Kawde, R. Polsky, Metal nanoparticle-based electrochemical stripping potentiometric detection of DNA hybridization, Anal. Chem., 73, 2001, pp. 5576–5581. 130
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