improved facial surface sebum and pore appearance - P&G Beauty ...

Methods
• In Vitro Sebocyte Assay:
– In vitro sebocytes have been used historically to evaluate effects of compounds on sebum
expression, including sebosuppression (6, 7). Lipid synthesis was detected via incorporation of
radiolabeled glucose over a 3 hour period in the presence of test compound in cultured
sebocytes (6, 7).
• Clinical Studies:
– Niacinamide Product Studies. Two 4-week studies were conducted with a niacinamide (2%)
containing facial moisturizer in Caucasian (U.S) and Japanese female panelists. The cosmetic
product was applied twice daily to the face and compared to the moisturizer base vehicle
control in a double-blind, vehicle-controlled split-face randomized (left-right) study design
(Caucasian study), and as well as compared to baseline (both studies). Facial surface sebum
(Sebutape®) and pore appearance (image analysis) were determined at baseline and up to 4
weeks. Self-assessments of improvements in facial skin appearance were obtained also.
Comparison of treatment groups was accomplished by t-test analysis.
– SDA Product Study. A double-blind study design similar to the that used for the niacinamide
studies was used to evaluate a facial moisturizer containing 0.5% SDA in combination with
3.5% niacinamide as compared to the moisturizer base vehicle control moisturizer. The
panelists were Caucasian (U.S.) women in a 6-week vehicle-controlled split-face randomized
(left-right) study design, evaluating sebum (Sebumeter) and pore (image analysis) endpoints.
Self-assessments of improvements in facial skin appearance were obtained also. Comparison
of treatment groups was accomplished by t-test analysis.